AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022291.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022291.01.T01 MTR_7g074790 70.130 77 23 0 1 77 1 77 1.24e-33 116
MsG0480022291.01.T01 MTR_5g039760 56.627 83 36 0 1 83 1 83 9.68e-30 105
MsG0480022291.01.T01 MTR_7g061980 57.831 83 35 0 1 83 1 83 1.51e-29 102
MsG0480022291.01.T01 MTR_4g069880 61.765 68 26 0 1 68 1 68 2.08e-21 80.5
MsG0480022291.01.T01 MTR_4g102720 49.206 63 32 0 7 69 4 66 9.15e-15 67.4
MsG0480022291.01.T01 MTR_0137s0040 52.459 61 28 1 2 61 28 88 9.85e-15 64.7
MsG0480022291.01.T01 MTR_6g017200 45.946 74 36 2 10 79 58 131 3.38e-14 64.3
MsG0480022291.01.T01 MTR_1g057920 42.857 77 40 2 10 82 34 110 2.83e-12 58.9
MsG0480022291.01.T01 MTR_2g070590 43.243 74 38 2 10 79 17 90 5.46e-12 57.8
MsG0480022291.01.T01 MTR_5g015480 49.180 61 30 1 10 69 56 116 6.32e-12 60.1
MsG0480022291.01.T01 MTR_0402s0040 43.243 74 38 2 10 79 26 99 8.64e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGTTACTTTGGATTCTATT+TGG 0.246811 4:+70718727 MsG0480022291.01.T01:CDS
TATAGTTTACGTGCGGGCTT+TGG 0.344757 4:+70718549 MsG0480022291.01.T01:CDS
TGGAATCTTCAAATGTTGAT+TGG 0.360826 4:+70718466 MsG0480022291.01.T01:CDS
TTGGAATGGAATTTGACAAT+AGG 0.381885 4:+70718499 MsG0480022291.01.T01:CDS
ACATGATGAAATAGTTACTT+TGG 0.389119 4:+70718716 MsG0480022291.01.T01:CDS
TGGAATGGAATTTGACAATA+GGG 0.410954 4:+70718500 MsG0480022291.01.T01:CDS
GGAATTTGACAATAGGGATA+AGG 0.423830 4:+70718506 MsG0480022291.01.T01:CDS
TCAAATTCCATTCCAAGTCT+TGG 0.427302 4:-70718492 None:intergenic
ATGTAGGTTTGTTTGTTGTA+AGG 0.436082 4:+70718620 MsG0480022291.01.T01:CDS
AGGTTTGTTTGTTGTAAGGA+AGG 0.474808 4:+70718624 MsG0480022291.01.T01:CDS
GTTGATTGGAAGCCAAGACT+TGG 0.497681 4:+70718480 MsG0480022291.01.T01:CDS
TTGGAAGCCAAGACTTGGAA+TGG 0.513444 4:+70718485 MsG0480022291.01.T01:CDS
GGTTTGTTTGTTGTAAGGAA+GGG 0.530392 4:+70718625 MsG0480022291.01.T01:CDS
ACGGTAGTATATCGTCATGT+AGG 0.554088 4:+70718604 MsG0480022291.01.T01:CDS
AAAGTAACTATTTCATCATG+TGG 0.568702 4:-70718714 None:intergenic
TTTGTTGTAAGGAAGGGCTA+CGG 0.581016 4:+70718631 MsG0480022291.01.T01:CDS
GTTGCTTATAGTTTACGTGC+GGG 0.585073 4:+70718543 MsG0480022291.01.T01:CDS
TGTTGCTTATAGTTTACGTG+CGG 0.599027 4:+70718542 MsG0480022291.01.T01:CDS
AATGCAAACAAGAAGAAAGA+CGG 0.644809 4:+70718585 MsG0480022291.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGTAACTATTTCATCATG+TGG - Chr4:70718717-70718736 None:intergenic 25.0%
! ACATGATGAAATAGTTACTT+TGG + Chr4:70718716-70718735 MsG0480022291.01.T01:CDS 25.0%
!! TAGTTACTTTGGATTCTATT+TGG + Chr4:70718727-70718746 MsG0480022291.01.T01:CDS 25.0%
AATGCAAACAAGAAGAAAGA+CGG + Chr4:70718585-70718604 MsG0480022291.01.T01:CDS 30.0%
CAAGGAAAAATAGTTAGAAC+AGG + Chr4:70718678-70718697 MsG0480022291.01.T01:CDS 30.0%
TGGAATCTTCAAATGTTGAT+TGG + Chr4:70718466-70718485 MsG0480022291.01.T01:CDS 30.0%
TGGAATGGAATTTGACAATA+GGG + Chr4:70718500-70718519 MsG0480022291.01.T01:CDS 30.0%
TTGGAATGGAATTTGACAAT+AGG + Chr4:70718499-70718518 MsG0480022291.01.T01:CDS 30.0%
!! ATGTAGGTTTGTTTGTTGTA+AGG + Chr4:70718620-70718639 MsG0480022291.01.T01:CDS 30.0%
GAGAAAAAAGATGCACATCA+AGG + Chr4:70718660-70718679 MsG0480022291.01.T01:CDS 35.0%
GGAATTTGACAATAGGGATA+AGG + Chr4:70718506-70718525 MsG0480022291.01.T01:CDS 35.0%
TCAAATTCCATTCCAAGTCT+TGG - Chr4:70718495-70718514 None:intergenic 35.0%
! TGTTGCTTATAGTTTACGTG+CGG + Chr4:70718542-70718561 MsG0480022291.01.T01:CDS 35.0%
!! AGGTTTGTTTGTTGTAAGGA+AGG + Chr4:70718624-70718643 MsG0480022291.01.T01:CDS 35.0%
!! GGTTTGTTTGTTGTAAGGAA+GGG + Chr4:70718625-70718644 MsG0480022291.01.T01:CDS 35.0%
ACGGTAGTATATCGTCATGT+AGG + Chr4:70718604-70718623 MsG0480022291.01.T01:CDS 40.0%
GTTGCTTATAGTTTACGTGC+GGG + Chr4:70718543-70718562 MsG0480022291.01.T01:CDS 40.0%
! TTTGTTGTAAGGAAGGGCTA+CGG + Chr4:70718631-70718650 MsG0480022291.01.T01:CDS 40.0%
GTTGATTGGAAGCCAAGACT+TGG + Chr4:70718480-70718499 MsG0480022291.01.T01:CDS 45.0%
TATAGTTTACGTGCGGGCTT+TGG + Chr4:70718549-70718568 MsG0480022291.01.T01:CDS 45.0%
TTGGAAGCCAAGACTTGGAA+TGG + Chr4:70718485-70718504 MsG0480022291.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70718465 70718776 70718465 ID=MsG0480022291.01;Name=MsG0480022291.01
Chr4 mRNA 70718465 70718776 70718465 ID=MsG0480022291.01.T01;Parent=MsG0480022291.01;Name=MsG0480022291.01.T01;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|0|1|0|103
Chr4 exon 70718465 70718776 70718465 ID=MsG0480022291.01.T01:exon:19172;Parent=MsG0480022291.01.T01
Chr4 CDS 70718465 70718776 70718465 ID=MsG0480022291.01.T01:cds;Parent=MsG0480022291.01.T01
Gene Sequence

>MsG0480022291.01.T01

ATGGAATCTTCAAATGTTGATTGGAAGCCAAGACTTGGAATGGAATTTGACAATAGGGATAAGGCTTATCAATTTTATGTTGCTTATAGTTTACGTGCGGGCTTTGGAGTTAGAAAACGTAATGCAAACAAGAAGAAAGACGGTAGTATATCGTCATGTAGGTTTGTTTGTTGTAAGGAAGGGCTACGGAATAAAGAGAAAAAAGATGCACATCAAGGAAAAATAGTTAGAACAGGTGTAAGTAGTGATCCACATGATGAAATAGTTACTTTGGATTCTATTTGGAGTTTCTTTGTAATCTTACTCCTCTAA
Protein sequence

>MsG0480022291.01.T01

MESSNVDWKPRLGMEFDNRDKAYQFYVAYSLRAGFGVRKRNANKKKDGSISSCRFVCCKEGLRNKEKKDAHQGKIVRTGVSSDPHDEIVTLDSIWSFFVILLL*