AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022828.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022828.01.T01 MTR_4g094762 75.649 501 89 8 1 487 1 482 0.0 673
MsG0480022828.01.T01 MTR_3g498695 27.801 241 139 6 263 475 243 476 3.88e-17 84.3
MsG0480022828.01.T01 MTR_3g498695 27.686 242 139 7 263 475 243 477 1.03e-15 79.7
MsG0480022828.01.T01 MTR_0194s0050 25.641 195 128 3 298 477 186 378 5.50e-14 73.9
MsG0480022828.01.T01 MTR_1g056700 70.213 47 13 1 275 320 8 54 3.56e-13 65.5
MsG0480022828.01.T01 MTR_1g072320 31.613 155 97 4 290 438 458 609 3.84e-12 68.9
MsG0480022828.01.T01 MTR_8g067280 29.677 155 104 2 291 442 456 608 5.15e-11 65.5
MsG0480022828.01.T01 MTR_5g030430 28.571 196 124 5 291 476 487 676 8.11e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022828.01.T01 AT2G31220 35.203 517 218 15 16 488 2 445 2.80e-73 239
MsG0480022828.01.T01 AT2G31220 35.135 518 219 15 15 488 12 456 4.16e-73 238
MsG0480022828.01.T01 AT2G31210 33.728 507 224 15 11 487 1 425 4.84e-71 232
MsG0480022828.01.T01 AT1G06170 40.278 360 152 9 144 488 100 411 9.95e-71 231
MsG0480022828.01.T01 AT1G06170 40.278 360 152 9 144 488 100 411 9.95e-71 231
MsG0480022828.01.T01 AT1G32640 29.814 161 86 5 291 442 446 588 5.50e-14 75.1
MsG0480022828.01.T01 AT5G46760 32.903 155 92 4 291 442 409 554 2.45e-12 69.7
MsG0480022828.01.T01 AT4G17880 30.921 152 95 2 291 442 410 551 5.24e-12 68.6
MsG0480022828.01.T01 AT3G24140 25.373 201 133 4 298 482 199 398 5.90e-12 67.8
MsG0480022828.01.T01 AT3G24140 25.373 201 133 4 298 482 199 398 7.22e-12 67.4
MsG0480022828.01.T01 AT1G12860 25.882 170 108 3 296 458 266 424 1.66e-11 66.6
MsG0480022828.01.T01 AT5G65640 29.480 173 104 4 290 462 171 325 2.00e-11 65.9
MsG0480022828.01.T01 AT1G12860 25.253 198 127 5 296 485 266 450 4.79e-11 65.1
MsG0480022828.01.T01 AT3G26744 25.616 203 124 6 296 485 306 494 5.99e-11 65.1
MsG0480022828.01.T01 AT3G26744 25.616 203 124 6 296 485 306 494 5.99e-11 65.1
MsG0480022828.01.T01 AT3G26744 25.616 203 124 6 296 485 306 494 5.99e-11 65.1
MsG0480022828.01.T01 AT2G31215 50.725 69 33 1 289 357 16 83 6.51e-11 60.5

Find 130 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTACTTTGGTGGAATT+TGG 0.152678 4:+77219982 MsG0480022829.01.T01:intron
CAGTTTGCTCATGCATATTT+TGG 0.176939 4:+77220919 MsG0480022829.01.T01:intron
AGGGATTGTAGCTTTGTTTA+TGG 0.188979 4:+77220496 MsG0480022829.01.T01:intron
CACTTGATTTGGCATGGTTT+GGG 0.215941 4:+77220833 MsG0480022829.01.T01:intron
CAACAACATGGATAACATTT+TGG 0.230373 4:-77220607 MsG0480022828.01.T01:CDS
GAACCTTCCTGCACAGTTTC+CGG 0.273029 4:-77220247 MsG0480022828.01.T01:CDS
CCACTTGATTTGGCATGGTT+TGG 0.275899 4:+77220832 MsG0480022829.01.T01:intron
TGTGTAGTTGGTGGTATTCT+TGG 0.280012 4:+77220520 MsG0480022829.01.T01:intron
GCTGTTTGTTTCCAAGGTTT+TGG 0.282123 4:-77219352 MsG0480022828.01.T01:CDS
AGTTATAGTGATACTTTCAT+AGG 0.284954 4:-77218977 MsG0480022828.01.T01:three_prime_UTR
ATAGCATTGAAGAGAATTTC+AGG 0.287093 4:-77220791 MsG0480022828.01.T01:CDS
TCAAGGAATGGTTCTCGTTT+TGG 0.293055 4:-77220171 MsG0480022828.01.T01:CDS
AGACCAATGAGTTCAGTTTC+AGG 0.294029 4:-77220948 None:intergenic
TATTTACTTTGGTGGAATTT+GGG 0.298058 4:+77219983 MsG0480022829.01.T01:intron
CTCATCGAGTAATGGAAATT+AGG 0.322097 4:+77220345 MsG0480022829.01.T01:intron
TTACCTGCAACCACTTGATT+TGG 0.325403 4:+77220822 MsG0480022829.01.T01:intron
TGGGGAGAGTTATTATCTTC+TGG 0.329534 4:+77220852 MsG0480022829.01.T01:intron
TTTGTTTATGGTTGTGTAGT+TGG 0.331934 4:+77220508 MsG0480022829.01.T01:intron
CAGCAGTTGTCCCACATAAT+AGG 0.332222 4:-77219082 MsG0480022828.01.T01:CDS
GAGATTGAGGCAATTCTCTA+AGG 0.339121 4:+77220223 MsG0480022829.01.T01:intron
TGTAGGTTGTAGTCAGTATT+AGG 0.349173 4:-77219058 MsG0480022828.01.T01:exon
AGCTGTCACATGAAGCCTTT+TGG 0.351110 4:-77219501 MsG0480022828.01.T01:CDS
AATGTCAGTACTAATTAATC+TGG 0.351847 4:+77219033 MsG0480022829.01.T01:intron
CTTGAGTTGTCTGAGCCTAT+TGG 0.353217 4:+77220306 MsG0480022829.01.T01:intron
AACATTGCACAACAAAATCT+TGG 0.361426 4:-77220654 MsG0480022828.01.T01:CDS
GGACCCTAGTGCTCAGGATA+TGG 0.374887 4:-77220556 MsG0480022828.01.T01:CDS
TTGGAACTTAACCATGTTGC+AGG 0.378869 4:-77219318 MsG0480022828.01.T01:CDS
TTGCTTGCTGTTTGTTTCCA+AGG 0.384323 4:-77219358 MsG0480022828.01.T01:CDS
TATCAACCTTGCTATTGAAC+AGG 0.396384 4:+77219268 MsG0480022829.01.T01:intron
TTGGTGGTATTCTTGGTGAT+TGG 0.402907 4:+77220527 MsG0480022829.01.T01:intron
ATGAGGGTCTTGAATGGTTG+AGG 0.403582 4:+77220476 MsG0480022829.01.T01:intron
TTTATTGTTATTTACTTTGG+TGG 0.406527 4:+77219975 MsG0480022829.01.T01:intron
CAAAATATGCATGAGCAAAC+TGG 0.407375 4:-77220918 MsG0480022828.01.T01:CDS
CAAAATCTTGGTTTGAACAT+AGG 0.414981 4:-77220642 MsG0480022828.01.T01:CDS
TGGTGAAGATTTAGTACATC+TGG 0.421373 4:+77220444 MsG0480022829.01.T01:intron
ATGGAGGGAGATGGAGGTAT+GGG 0.422492 4:-77220135 MsG0480022828.01.T01:CDS
GGGGATGAGATGGAGGGAGA+TGG 0.422522 4:-77220144 MsG0480022828.01.T01:CDS
ATGCAAGTGCTATAGCAAAC+AGG 0.424664 4:-77219133 MsG0480022828.01.T01:CDS
ATAGATGAAGCTGAGGATCT+AGG 0.426468 4:+77220417 MsG0480022829.01.T01:intron
GATGGAGGGAGATGGAGGTA+TGG 0.426821 4:-77220136 MsG0480022828.01.T01:CDS
ATGCCAAATCAAGTGGTTGC+AGG 0.427884 4:-77220825 MsG0480022828.01.T01:CDS
TTTCTTGGAGACCTTCCAAT+AGG 0.433519 4:-77220321 MsG0480022828.01.T01:CDS
AAAATCTTGGTTTGAACATA+GGG 0.440034 4:-77220641 MsG0480022828.01.T01:CDS
AAACAGGGTGATTGATGTTC+TGG 0.444364 4:-77219117 MsG0480022828.01.T01:CDS
TGGTGGAGGGGATGAGATGG+AGG 0.452096 4:-77220151 MsG0480022828.01.T01:CDS
GATGTCTCCACTTATACAAA+TGG 0.460153 4:-77220696 MsG0480022828.01.T01:CDS
GAGGTCCTTATGCTTCCATC+TGG 0.463883 4:+77219474 MsG0480022829.01.T01:intron
AGTGAATCCATTTGTATAAG+TGG 0.466255 4:+77220689 MsG0480022829.01.T01:intron
TCCGATGCAAAATTGTCGTC+GGG 0.468275 4:-77218858 MsG0480022828.01.T01:three_prime_UTR
AGACATCTTCAAGGTGTTGT+GGG 0.477092 4:+77220712 MsG0480022829.01.T01:intron
ACAACCTACATCCTATTATG+TGG 0.478099 4:+77219071 MsG0480022829.01.T01:intron
GATGAGATGGAGGGAGATGG+AGG 0.485707 4:-77220141 MsG0480022828.01.T01:CDS
CAAACTGTTTCAGAGAAAGA+AGG 0.486584 4:-77219385 MsG0480022828.01.T01:CDS
AACTGTGCAGGAAGGTTCAT+AGG 0.487200 4:+77220252 MsG0480022829.01.T01:intron
AGTTGGGGTTGTGATGCAAA+TGG 0.488283 4:+77220377 MsG0480022829.01.T01:intron
ACAAAGAAGAGAGCAATTGA+AGG 0.497369 4:-77220034 MsG0480022828.01.T01:CDS
CTAAGGGCCGGAAACTGTGC+AGG 0.501150 4:+77220240 MsG0480022829.01.T01:intron
TGAGGGTCTTGAATGGTTGA+GGG 0.501208 4:+77220477 MsG0480022829.01.T01:intron
CAAGAAAACTCATCGAGTAA+TGG 0.504530 4:+77220337 MsG0480022829.01.T01:intron
TGCAACCACTTGATTTGGCA+TGG 0.510199 4:+77220827 MsG0480022829.01.T01:intron
CTTCCATCTGGATCACCAAA+AGG 0.510562 4:+77219486 MsG0480022829.01.T01:intron
AGATGGAGGTATGGGAGTGT+CGG 0.512294 4:-77220127 MsG0480022828.01.T01:CDS
GGAAGGTTCATAGGAAATAG+AGG 0.514568 4:+77220261 MsG0480022829.01.T01:intron
GAGACATCTTCAAGGTGTTG+TGG 0.514897 4:+77220711 MsG0480022829.01.T01:intron
CTGAAGTTCCTCCAAAACCT+TGG 0.519653 4:+77219341 MsG0480022829.01.T01:CDS
TAGTTTCCTGTTCAATAGCA+AGG 0.522557 4:-77219274 MsG0480022828.01.T01:intron
ACGAGAACCATTCCTTGAGT+TGG 0.523595 4:+77220176 MsG0480022829.01.T01:CDS
TCTCCTGAAACTGAACTCAT+TGG 0.525396 4:+77220945 MsG0480022829.01.T01:intron
AGTGAATGAGCTGAAACTAT+TGG 0.528670 4:-77219589 MsG0480022828.01.T01:CDS
GGTGTATCAGAATTCGAGTT+GGG 0.534079 4:-77220577 MsG0480022828.01.T01:CDS
TAGTCCATATCCTGAGCACT+AGG 0.537172 4:+77220552 MsG0480022829.01.T01:intron
GTTTATGGTTGTGTAGTTGG+TGG 0.542610 4:+77220511 MsG0480022829.01.T01:intron
AGAGAATTTCAGGCCTTCTG+TGG 0.547008 4:-77220781 MsG0480022828.01.T01:CDS
TAGACGTGAGGGAGAGATTG+AGG 0.548170 4:+77220210 MsG0480022829.01.T01:intron
GTTGTGATGCAAATGGTGGA+TGG 0.549380 4:+77220384 MsG0480022829.01.T01:intron
GTTATAGTGATACTTTCATA+GGG 0.550492 4:-77218976 MsG0480022828.01.T01:three_prime_UTR
TATAAGTGGAGACATCTTCA+AGG 0.550747 4:+77220703 MsG0480022829.01.T01:intron
AAGACAGTGAGGTTGATGTT+CGG 0.551962 4:-77219431 MsG0480022828.01.T01:CDS
GAGTTGGGACCCTAGTGCTC+AGG 0.552970 4:-77220562 MsG0480022828.01.T01:CDS
AACACCTTCTGCCATTGTGT+CGG 0.553819 4:+77220884 MsG0480022829.01.T01:intron
GTGATCCAGATGGAAGCATA+AGG 0.554959 4:-77219479 MsG0480022828.01.T01:CDS
ACATCTGGTGAAGGATAATG+AGG 0.556346 4:+77220459 MsG0480022829.01.T01:intron
GGTGGAGGGGATGAGATGGA+GGG 0.559231 4:-77220150 MsG0480022828.01.T01:CDS
GGAGAAGAAAAGACACGAGA+TGG 0.559458 4:-77219565 MsG0480022828.01.T01:CDS
TGAGGCAATTCTCTAAGGGC+CGG 0.565775 4:+77220228 MsG0480022829.01.T01:intron
ACTTGATTTGGCATGGTTTG+GGG 0.567633 4:+77220834 MsG0480022829.01.T01:intron
GAACCATTCCTTGAGTTGGT+AGG 0.569140 4:+77220180 MsG0480022829.01.T01:intron
ATGGAAGCATAAGGACCTCA+TGG 0.569401 4:-77219470 MsG0480022828.01.T01:CDS
TCACTTATGCAGGTAACTGA+AGG 0.570501 4:-77219167 MsG0480022828.01.T01:intron
GAGAATTTCAGGCCTTCTGT+GGG 0.572554 4:-77220780 MsG0480022828.01.T01:CDS
TCAAAATCTCTTCCCACAGA+AGG 0.573360 4:+77220768 MsG0480022829.01.T01:intron
TTCCGATGCAAAATTGTCGT+CGG 0.575752 4:-77218859 MsG0480022828.01.T01:three_prime_UTR
CCAAACCATGCCAAATCAAG+TGG 0.578899 4:-77220832 MsG0480022828.01.T01:CDS
TGTCCCACATAATAGGATGT+AGG 0.580034 4:-77219075 MsG0480022828.01.T01:exon
TACTTTCATAGGGAACATCT+AGG 0.581729 4:-77218966 MsG0480022828.01.T01:three_prime_UTR
AGGATAATGAGGGTCTTGAA+TGG 0.581908 4:+77220470 MsG0480022829.01.T01:intron
TGGGGTTGTGATGCAAATGG+TGG 0.587727 4:+77220380 MsG0480022829.01.T01:intron
CAACCTACATCCTATTATGT+GGG 0.595003 4:+77219072 MsG0480022829.01.T01:intron
GATTCCGACACAATGGCAGA+AGG 0.597510 4:-77220888 MsG0480022828.01.T01:CDS
ATGCCTACCAACTCAAGGAA+TGG 0.597546 4:-77220183 MsG0480022828.01.T01:CDS
CAAAGAAGAGAGCAATTGAA+GGG 0.598165 4:-77220033 MsG0480022828.01.T01:CDS
TAAGGACCTCATGGCTGCAG+AGG 0.598266 4:-77219461 MsG0480022828.01.T01:CDS
TGATTTCCTCTGCAGCCATG+AGG 0.604351 4:+77219455 MsG0480022829.01.T01:intron
AAGCTGAGGATCTAGGAAAG+TGG 0.606657 4:+77220424 MsG0480022829.01.T01:intron
GAACTTAACCATGTTGCAGG+TGG 0.608004 4:-77219315 MsG0480022828.01.T01:CDS
AGATTGAGGCAATTCTCTAA+GGG 0.609395 4:+77220224 MsG0480022829.01.T01:intron
AGTTGTCTGAGCCTATTGGA+AGG 0.611422 4:+77220310 MsG0480022829.01.T01:intron
ACAGGATGACAGAGCATCAG+TGG 0.612086 4:-77219652 MsG0480022828.01.T01:intron
CAACATGTCCACCTGCAACA+TGG 0.612887 4:+77219307 MsG0480022829.01.T01:CDS
CATGTTGCAGGTGGACATGT+TGG 0.614636 4:-77219306 MsG0480022828.01.T01:CDS
TGCAAGTGCTATAGCAAACA+GGG 0.617014 4:-77219132 MsG0480022828.01.T01:CDS
ATAGCATGCCTACCAACTCA+AGG 0.617097 4:-77220188 MsG0480022828.01.T01:CDS
TGTGAGAATAGATGAAGCTG+AGG 0.619586 4:+77220410 MsG0480022829.01.T01:intron
AGATTTAGTACATCTGGTGA+AGG 0.621346 4:+77220450 MsG0480022829.01.T01:intron
GAATGAGCTGAAACTATTGG+TGG 0.621989 4:-77219586 MsG0480022828.01.T01:CDS
AGGTGTATCAGAATTCGAGT+TGG 0.627894 4:-77220578 MsG0480022828.01.T01:CDS
AGGGAAGATACAAAATCTTG+AGG 0.628748 4:-77220014 MsG0480022828.01.T01:CDS
ATAGGCTCAGACAACTCAAG+TGG 0.630249 4:-77220303 MsG0480022828.01.T01:CDS
ATCGAGTAATGGAAATTAGG+TGG 0.631613 4:+77220348 MsG0480022829.01.T01:intron
GATGACAGAGCATCAGTGGT+CGG 0.636237 4:-77219648 MsG0480022828.01.T01:CDS
GGGCCGGAAACTGTGCAGGA+AGG 0.638113 4:+77220244 MsG0480022829.01.T01:intron
AGTCCATATCCTGAGCACTA+GGG 0.638599 4:+77220553 MsG0480022829.01.T01:intron
GGGAGTGTCGGAATTCAACA+AGG 0.647586 4:-77220115 MsG0480022828.01.T01:CDS
TAGGCATGCTATAGACGTGA+GGG 0.648613 4:+77220199 MsG0480022829.01.T01:intron
AGCTACAGAACATTTGACAA+CGG 0.658358 4:-77220061 MsG0480022828.01.T01:CDS
GTAGGCATGCTATAGACGTG+AGG 0.659082 4:+77220198 MsG0480022829.01.T01:intron
TTGCTTTGATTCCGACACAA+TGG 0.659636 4:-77220895 MsG0480022828.01.T01:CDS
CATCTGGTGAAGGATAATGA+GGG 0.694690 4:+77220460 MsG0480022829.01.T01:intron
GAACTACTACTACAACAACA+TGG 0.699767 4:-77220619 MsG0480022828.01.T01:CDS
GAGGAAATCAAAAGACAGTG+AGG 0.707672 4:-77219442 MsG0480022828.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATAAAGTATTTACT+TGG + Chr4:77220500-77220519 MsG0480022829.01.T01:intron 10.0%
!!! TTATTTATTGTTATTTACTT+TGG + Chr4:77219770-77219789 MsG0480022829.01.T01:intron 10.0%
!! ACCATGATTTAAATAATTAA+CGG - Chr4:77220048-77220067 MsG0480022828.01.T01:CDS 15.0%
!! ATAAGATTCAAATCATTATT+TGG - Chr4:77219990-77220009 MsG0480022828.01.T01:CDS 15.0%
!! GTTAAAAATGACATTATTAT+TGG - Chr4:77219946-77219965 MsG0480022828.01.T01:intron 15.0%
!! TAGATTTATTACATTTGATT+TGG - Chr4:77220526-77220545 MsG0480022828.01.T01:CDS 15.0%
!! TTCATAGATATAATTAGATA+TGG + Chr4:77219812-77219831 MsG0480022829.01.T01:intron 15.0%
!!! TTAAATCTTGAATTATTTTG+AGG - Chr4:77219873-77219892 MsG0480022828.01.T01:intron 15.0%
!! CCATGATTTAAATAATTAAC+GGG - Chr4:77220049-77220068 MsG0480022828.01.T01:CDS 20.0%
!! TTGGTTATATATTAACTATG+AGG - Chr4:77220545-77220564 MsG0480022828.01.T01:CDS 20.0%
!!! TTTATTGTTATTTACTTTGG+TGG + Chr4:77219767-77219786 MsG0480022829.01.T01:intron 20.0%
! AATGTCAGTACTAATTAATC+TGG + Chr4:77220709-77220728 MsG0480022829.01.T01:intron 25.0%
! AGTTATAGTGATACTTTCAT+AGG - Chr4:77220762-77220781 MsG0480022828.01.T01:CDS 25.0%
! CCCGTTAATTATTTAAATCA+TGG + Chr4:77220052-77220071 MsG0480022829.01.T01:intron 25.0%
! GTTATAGTGATACTTTCATA+GGG - Chr4:77220763-77220782 MsG0480022828.01.T01:CDS 25.0%
! TATTTACTTTGGTGGAATTT+GGG + Chr4:77219759-77219778 MsG0480022829.01.T01:intron 25.0%
! TTATTTACTTTGGTGGAATT+TGG + Chr4:77219760-77219779 MsG0480022829.01.T01:intron 25.0%
!! AAAATCTTGGTTTGAACATA+GGG - Chr4:77219098-77219117 MsG0480022828.01.T01:CDS 25.0%
!!! TTCTTTTTTATGGTACTTTG+TGG - Chr4:77220836-77220855 MsG0480022828.01.T01:CDS 25.0%
AACATTGCACAACAAAATCT+TGG - Chr4:77219085-77219104 MsG0480022828.01.T01:CDS 30.0%
AGTGAATCCATTTGTATAAG+TGG + Chr4:77219053-77219072 MsG0480022829.01.T01:intron 30.0%
ATCTAATGACAAAAGCAAAG+TGG + Chr4:77219846-77219865 MsG0480022829.01.T01:intron 30.0%
TTTGTTTATGGTTGTGTAGT+TGG + Chr4:77219234-77219253 MsG0480022829.01.T01:intron 30.0%
! ATAGCATTGAAGAGAATTTC+AGG - Chr4:77218948-77218967 MsG0480022828.01.T01:three_prime_UTR 30.0%
! CAAAATCTTGGTTTGAACAT+AGG - Chr4:77219097-77219116 MsG0480022828.01.T01:CDS 30.0%
! CAACAACATGGATAACATTT+TGG - Chr4:77219132-77219151 MsG0480022828.01.T01:CDS 30.0%
! TTGTTATGTCACTTGTAGTT+TGG - Chr4:77219924-77219943 MsG0480022828.01.T01:intron 30.0%
!!! CTTTGCGTCTTTCTTTTTTA+TGG - Chr4:77220826-77220845 MsG0480022828.01.T01:CDS 30.0%
ACAAAGAAGAGAGCAATTGA+AGG - Chr4:77219705-77219724 MsG0480022828.01.T01:intron 35.0%
ACAACCTACATCCTATTATG+TGG + Chr4:77220671-77220690 MsG0480022829.01.T01:intron 35.0%
AGATTGAGGCAATTCTCTAA+GGG + Chr4:77219518-77219537 MsG0480022829.01.T01:intron 35.0%
AGATTTAGTACATCTGGTGA+AGG + Chr4:77219292-77219311 MsG0480022829.01.T01:CDS 35.0%
AGCTACAGAACATTTGACAA+CGG - Chr4:77219678-77219697 MsG0480022828.01.T01:intron 35.0%
AGGGAAGATACAAAATCTTG+AGG - Chr4:77219725-77219744 MsG0480022828.01.T01:intron 35.0%
AGTGAATGAGCTGAAACTAT+TGG - Chr4:77220150-77220169 MsG0480022828.01.T01:CDS 35.0%
CAAAATATGCATGAGCAAAC+TGG - Chr4:77218821-77218840 MsG0480022828.01.T01:three_prime_UTR 35.0%
CAAACTGTTTCAGAGAAAGA+AGG - Chr4:77220354-77220373 MsG0480022828.01.T01:CDS 35.0%
CAAAGAAGAGAGCAATTGAA+GGG - Chr4:77219706-77219725 MsG0480022828.01.T01:intron 35.0%
CAACCTACATCCTATTATGT+GGG + Chr4:77220670-77220689 MsG0480022829.01.T01:intron 35.0%
CAAGAAAACTCATCGAGTAA+TGG + Chr4:77219405-77219424 MsG0480022829.01.T01:CDS 35.0%
GAACTACTACTACAACAACA+TGG - Chr4:77219120-77219139 MsG0480022828.01.T01:CDS 35.0%
GATGTCTCCACTTATACAAA+TGG - Chr4:77219043-77219062 MsG0480022828.01.T01:three_prime_UTR 35.0%
TACTTTCATAGGGAACATCT+AGG - Chr4:77220773-77220792 MsG0480022828.01.T01:CDS 35.0%
TAGTTTCCTGTTCAATAGCA+AGG - Chr4:77220465-77220484 MsG0480022828.01.T01:CDS 35.0%
TATAAGTGGAGACATCTTCA+AGG + Chr4:77219039-77219058 MsG0480022829.01.T01:intron 35.0%
TGGTGAAGATTTAGTACATC+TGG + Chr4:77219298-77219317 MsG0480022829.01.T01:CDS 35.0%
TGTAGGTTGTAGTCAGTATT+AGG - Chr4:77220681-77220700 MsG0480022828.01.T01:CDS 35.0%
! ATCGAGTAATGGAAATTAGG+TGG + Chr4:77219394-77219413 MsG0480022829.01.T01:CDS 35.0%
! CATTACTCGATGAGTTTTCT+TGG - Chr4:77219403-77219422 MsG0480022828.01.T01:CDS 35.0%
! CTCATCGAGTAATGGAAATT+AGG + Chr4:77219397-77219416 MsG0480022829.01.T01:CDS 35.0%
! TGTGCAATGTTTTCTGATGA+GGG + Chr4:77219076-77219095 MsG0480022829.01.T01:intron 35.0%
! TTGTGCAATGTTTTCTGATG+AGG + Chr4:77219077-77219096 MsG0480022829.01.T01:intron 35.0%
!! AAATTAGGTGGCTTTTGAGT+TGG + Chr4:77219382-77219401 MsG0480022829.01.T01:CDS 35.0%
!! AGGGATTGTAGCTTTGTTTA+TGG + Chr4:77219246-77219265 MsG0480022829.01.T01:intron 35.0%
!! CAGTTTGCTCATGCATATTT+TGG + Chr4:77218823-77218842 MsG0480022829.01.T01:intron 35.0%
!! TATCAACCTTGCTATTGAAC+AGG + Chr4:77220474-77220493 MsG0480022829.01.T01:intron 35.0%
!! TGTCGTCGGGATTTTTAATT+TGG - Chr4:77220894-77220913 MsG0480022828.01.T01:CDS 35.0%
!! TGTGGGAAGAGATTTTGATA+AGG - Chr4:77218976-77218995 MsG0480022828.01.T01:three_prime_UTR 35.0%
!!! AATTAGGTGGCTTTTGAGTT+GGG + Chr4:77219381-77219400 MsG0480022829.01.T01:CDS 35.0%
!!! GTCGTCGGGATTTTTAATTT+GGG - Chr4:77220895-77220914 MsG0480022828.01.T01:CDS 35.0%
AAACAGGGTGATTGATGTTC+TGG - Chr4:77220622-77220641 MsG0480022828.01.T01:CDS 40.0%
ACATCTGGTGAAGGATAATG+AGG + Chr4:77219283-77219302 MsG0480022829.01.T01:intron 40.0%
AGGATAATGAGGGTCTTGAA+TGG + Chr4:77219272-77219291 MsG0480022829.01.T01:intron 40.0%
AGGTGTATCAGAATTCGAGT+TGG - Chr4:77219161-77219180 MsG0480022828.01.T01:intron 40.0%
ATAGATGAAGCTGAGGATCT+AGG + Chr4:77219325-77219344 MsG0480022829.01.T01:CDS 40.0%
ATGCAAGTGCTATAGCAAAC+AGG - Chr4:77220606-77220625 MsG0480022828.01.T01:CDS 40.0%
CATCTGGTGAAGGATAATGA+GGG + Chr4:77219282-77219301 MsG0480022829.01.T01:intron 40.0%
GAATGAGCTGAAACTATTGG+TGG - Chr4:77220153-77220172 MsG0480022828.01.T01:CDS 40.0%
GAGATTGAGGCAATTCTCTA+AGG + Chr4:77219519-77219538 MsG0480022829.01.T01:intron 40.0%
GAGGAAATCAAAAGACAGTG+AGG - Chr4:77220297-77220316 MsG0480022828.01.T01:CDS 40.0%
GGAAGGTTCATAGGAAATAG+AGG + Chr4:77219481-77219500 MsG0480022829.01.T01:intron 40.0%
GTTTATGGTTGTGTAGTTGG+TGG + Chr4:77219231-77219250 MsG0480022829.01.T01:intron 40.0%
TCAAAATCTCTTCCCACAGA+AGG + Chr4:77218974-77218993 MsG0480022829.01.T01:intron 40.0%
TCACTTATGCAGGTAACTGA+AGG - Chr4:77220572-77220591 MsG0480022828.01.T01:CDS 40.0%
TGCAAGTGCTATAGCAAACA+GGG - Chr4:77220607-77220626 MsG0480022828.01.T01:CDS 40.0%
TGGGGAGAGTTATTATCTTC+TGG + Chr4:77218890-77218909 MsG0480022829.01.T01:intron 40.0%
TGTCCCACATAATAGGATGT+AGG - Chr4:77220664-77220683 MsG0480022828.01.T01:CDS 40.0%
TGTGAGAATAGATGAAGCTG+AGG + Chr4:77219332-77219351 MsG0480022829.01.T01:CDS 40.0%
TTACCTGCAACCACTTGATT+TGG + Chr4:77218920-77218939 MsG0480022829.01.T01:intron 40.0%
TTCCGATGCAAAATTGTCGT+CGG - Chr4:77220880-77220899 MsG0480022828.01.T01:CDS 40.0%
TTGCTTTGATTCCGACACAA+TGG - Chr4:77218844-77218863 MsG0480022828.01.T01:three_prime_UTR 40.0%
TTGGAACTTAACCATGTTGC+AGG - Chr4:77220421-77220440 MsG0480022828.01.T01:CDS 40.0%
TTTCTTGGAGACCTTCCAAT+AGG - Chr4:77219418-77219437 MsG0480022828.01.T01:CDS 40.0%
! ATGAGGTGTTTCACTTATGC+AGG - Chr4:77220562-77220581 MsG0480022828.01.T01:CDS 40.0%
! GCTGTTTGTTTCCAAGGTTT+TGG - Chr4:77220387-77220406 MsG0480022828.01.T01:CDS 40.0%
! GCTTTTGTTGGCAAAGGAAA+AGG - Chr4:77219652-77219671 MsG0480022828.01.T01:intron 40.0%
! GGTGTATCAGAATTCGAGTT+GGG - Chr4:77219162-77219181 MsG0480022828.01.T01:intron 40.0%
! TCAAGGAATGGTTCTCGTTT+TGG - Chr4:77219568-77219587 MsG0480022828.01.T01:CDS 40.0%
! TTGGTGGTATTCTTGGTGAT+TGG + Chr4:77219215-77219234 MsG0480022829.01.T01:intron 40.0%
!! AACAAGGTCACTGCTTTTGT+TGG - Chr4:77219640-77219659 MsG0480022828.01.T01:CDS 40.0%
!! AAGACAGTGAGGTTGATGTT+CGG - Chr4:77220308-77220327 MsG0480022828.01.T01:CDS 40.0%
!! ACTTGATTTGGCATGGTTTG+GGG + Chr4:77218908-77218927 MsG0480022829.01.T01:intron 40.0%
!! AGACATCTTCAAGGTGTTGT+GGG + Chr4:77219030-77219049 MsG0480022829.01.T01:intron 40.0%
!! CACTTGATTTGGCATGGTTT+GGG + Chr4:77218909-77218928 MsG0480022829.01.T01:intron 40.0%
!! GGGAAGAGATTTTGATAAGG+AGG - Chr4:77218979-77218998 MsG0480022828.01.T01:three_prime_UTR 40.0%
!! GGGCAGTTTTGATCTAATAC+AGG - Chr4:77220069-77220088 MsG0480022828.01.T01:CDS 40.0%
!! GTTTGTTTCCAAGGTTTTGG+AGG - Chr4:77220390-77220409 MsG0480022828.01.T01:CDS 40.0%
!! TGTGTAGTTGGTGGTATTCT+TGG + Chr4:77219222-77219241 MsG0480022829.01.T01:intron 40.0%
!! TTGCTTGCTGTTTGTTTCCA+AGG - Chr4:77220381-77220400 MsG0480022828.01.T01:CDS 40.0%
!!! ATTAGGTGGCTTTTGAGTTG+GGG + Chr4:77219380-77219399 MsG0480022829.01.T01:CDS 40.0%
!!! GGATAACATTTTGGAGCAAG+AGG - Chr4:77219141-77219160 MsG0480022828.01.T01:CDS 40.0%
AACACCTTCTGCCATTGTGT+CGG + Chr4:77218858-77218877 MsG0480022829.01.T01:intron 45.0%
AACTGTGCAGGAAGGTTCAT+AGG + Chr4:77219490-77219509 MsG0480022829.01.T01:intron 45.0%
AAGCTGAGGATCTAGGAAAG+TGG + Chr4:77219318-77219337 MsG0480022829.01.T01:CDS 45.0%
ACGAGAACCATTCCTTGAGT+TGG + Chr4:77219566-77219585 MsG0480022829.01.T01:CDS 45.0%
AGAGAATTTCAGGCCTTCTG+TGG - Chr4:77218958-77218977 MsG0480022828.01.T01:three_prime_UTR 45.0%
AGTCCATATCCTGAGCACTA+GGG + Chr4:77219189-77219208 MsG0480022829.01.T01:intron 45.0%
AGTTGGGGTTGTGATGCAAA+TGG + Chr4:77219365-77219384 MsG0480022829.01.T01:CDS 45.0%
AGTTGTCTGAGCCTATTGGA+AGG + Chr4:77219432-77219451 MsG0480022829.01.T01:intron 45.0%
ATAGCATGCCTACCAACTCA+AGG - Chr4:77219551-77219570 MsG0480022828.01.T01:CDS 45.0%
ATAGGCTCAGACAACTCAAG+TGG - Chr4:77219436-77219455 MsG0480022828.01.T01:CDS 45.0%
ATGAGGGTCTTGAATGGTTG+AGG + Chr4:77219266-77219285 MsG0480022829.01.T01:intron 45.0%
ATGCCAAATCAAGTGGTTGC+AGG - Chr4:77218914-77218933 MsG0480022828.01.T01:three_prime_UTR 45.0%
ATGCCTACCAACTCAAGGAA+TGG - Chr4:77219556-77219575 MsG0480022828.01.T01:CDS 45.0%
CAGCAGTTGTCCCACATAAT+AGG - Chr4:77220657-77220676 MsG0480022828.01.T01:CDS 45.0%
CCAAACCATGCCAAATCAAG+TGG - Chr4:77218907-77218926 MsG0480022828.01.T01:three_prime_UTR 45.0%
CTGAAGTTCCTCCAAAACCT+TGG + Chr4:77220401-77220420 MsG0480022829.01.T01:intron 45.0%
CTTCCATCTGGATCACCAAA+AGG + Chr4:77220256-77220275 MsG0480022829.01.T01:intron 45.0%
GAACTTAACCATGTTGCAGG+TGG - Chr4:77220424-77220443 MsG0480022828.01.T01:CDS 45.0%
GAGAATTTCAGGCCTTCTGT+GGG - Chr4:77218959-77218978 MsG0480022828.01.T01:three_prime_UTR 45.0%
GGAGAAGAAAAGACACGAGA+TGG - Chr4:77220174-77220193 MsG0480022828.01.T01:CDS 45.0%
GTCTCCTGAAACTGAACTCA+TGG + Chr4:77218798-77218817 MsG0480022829.01.T01:intron 45.0%
TAGGCATGCTATAGACGTGA+GGG + Chr4:77219543-77219562 MsG0480022829.01.T01:intron 45.0%
TAGTCCATATCCTGAGCACT+AGG + Chr4:77219190-77219209 MsG0480022829.01.T01:intron 45.0%
TCCGATGCAAAATTGTCGTC+GGG - Chr4:77220881-77220900 MsG0480022828.01.T01:CDS 45.0%
TGAGGGTCTTGAATGGTTGA+GGG + Chr4:77219265-77219284 MsG0480022829.01.T01:intron 45.0%
! AGCTGTCACATGAAGCCTTT+TGG - Chr4:77220238-77220257 MsG0480022828.01.T01:CDS 45.0%
! AGGAATGGTTCTCGTTTTGG+TGG - Chr4:77219571-77219590 MsG0480022828.01.T01:CDS 45.0%
! ATGGAAGCATAAGGACCTCA+TGG - Chr4:77220269-77220288 MsG0480022828.01.T01:CDS 45.0%
! CTTGAGTTGTCTGAGCCTAT+TGG + Chr4:77219436-77219455 MsG0480022829.01.T01:intron 45.0%
! GTGATCCAGATGGAAGCATA+AGG - Chr4:77220260-77220279 MsG0480022828.01.T01:CDS 45.0%
! GTTGTGATGCAAATGGTGGA+TGG + Chr4:77219358-77219377 MsG0480022829.01.T01:CDS 45.0%
! TCCCGACGACAATTTTGCAT+CGG + Chr4:77220885-77220904 MsG0480022829.01.T01:intron 45.0%
!! AAGCCTTTTGGTGATCCAGA+TGG - Chr4:77220250-77220269 MsG0480022828.01.T01:CDS 45.0%
!! AATGGTTCTCGTTTTGGTGG+AGG - Chr4:77219574-77219593 MsG0480022828.01.T01:CDS 45.0%
!! ATGGTTCTCGTTTTGGTGGA+GGG - Chr4:77219575-77219594 MsG0480022828.01.T01:CDS 45.0%
!! CCACTTGATTTGGCATGGTT+TGG + Chr4:77218910-77218929 MsG0480022829.01.T01:intron 45.0%
!! GAACCATTCCTTGAGTTGGT+AGG + Chr4:77219562-77219581 MsG0480022829.01.T01:CDS 45.0%
!! GAGACATCTTCAAGGTGTTG+TGG + Chr4:77219031-77219050 MsG0480022829.01.T01:intron 45.0%
!! GTCACTGCTTTTGTTGGCAA+AGG - Chr4:77219646-77219665 MsG0480022828.01.T01:CDS 45.0%
!! TGCAACCACTTGATTTGGCA+TGG + Chr4:77218915-77218934 MsG0480022829.01.T01:intron 45.0%
!!! GGTTTTGGAGGAACTTCAGT+TGG - Chr4:77220402-77220421 MsG0480022828.01.T01:CDS 45.0%
CAACATGTCCACCTGCAACA+TGG + Chr4:77220435-77220454 MsG0480022829.01.T01:intron 50.0%
CATGTTGCAGGTGGACATGT+TGG - Chr4:77220433-77220452 MsG0480022828.01.T01:CDS 50.0%
GAACCTTCCTGCACAGTTTC+CGG - Chr4:77219492-77219511 MsG0480022828.01.T01:CDS 50.0%
GAGGTCCTTATGCTTCCATC+TGG + Chr4:77220268-77220287 MsG0480022829.01.T01:intron 50.0%
GATTCCGACACAATGGCAGA+AGG - Chr4:77218851-77218870 MsG0480022828.01.T01:three_prime_UTR 50.0%
GTAGGCATGCTATAGACGTG+AGG + Chr4:77219544-77219563 MsG0480022829.01.T01:intron 50.0%
TAGACGTGAGGGAGAGATTG+AGG + Chr4:77219532-77219551 MsG0480022829.01.T01:intron 50.0%
TGATTTCCTCTGCAGCCATG+AGG + Chr4:77220287-77220306 MsG0480022829.01.T01:intron 50.0%
TGGGGTTGTGATGCAAATGG+TGG + Chr4:77219362-77219381 MsG0480022829.01.T01:CDS 50.0%
! ACAGGATGACAGAGCATCAG+TGG - Chr4:77220087-77220106 MsG0480022828.01.T01:CDS 50.0%
! AGATGGAGGTATGGGAGTGT+CGG - Chr4:77219612-77219631 MsG0480022828.01.T01:CDS 50.0%
! ATGGAGGGAGATGGAGGTAT+GGG - Chr4:77219604-77219623 MsG0480022828.01.T01:CDS 50.0%
! GATGACAGAGCATCAGTGGT+CGG - Chr4:77220091-77220110 MsG0480022828.01.T01:CDS 50.0%
! TGAGGCAATTCTCTAAGGGC+CGG + Chr4:77219514-77219533 MsG0480022829.01.T01:intron 50.0%
! TTTTGGTGGAGGGGATGAGA+TGG - Chr4:77219585-77219604 MsG0480022828.01.T01:CDS 50.0%
!! GGGAGTGTCGGAATTCAACA+AGG - Chr4:77219624-77219643 MsG0480022828.01.T01:CDS 50.0%
!! TGGTTCTCGTTTTGGTGGAG+GGG - Chr4:77219576-77219595 MsG0480022828.01.T01:CDS 50.0%
GATGAGATGGAGGGAGATGG+AGG - Chr4:77219598-77219617 MsG0480022828.01.T01:CDS 55.0%
GATGGAGGGAGATGGAGGTA+TGG - Chr4:77219603-77219622 MsG0480022828.01.T01:CDS 55.0%
GGACCCTAGTGCTCAGGATA+TGG - Chr4:77219183-77219202 MsG0480022828.01.T01:intron 55.0%
TAAGGACCTCATGGCTGCAG+AGG - Chr4:77220278-77220297 MsG0480022828.01.T01:CDS 55.0%
CTAAGGGCCGGAAACTGTGC+AGG + Chr4:77219502-77219521 MsG0480022829.01.T01:intron 60.0%
GAGTTGGGACCCTAGTGCTC+AGG - Chr4:77219177-77219196 MsG0480022828.01.T01:intron 60.0%
GGGGATGAGATGGAGGGAGA+TGG - Chr4:77219595-77219614 MsG0480022828.01.T01:CDS 60.0%
GGTGGAGGGGATGAGATGGA+GGG - Chr4:77219589-77219608 MsG0480022828.01.T01:CDS 60.0%
TGGTGGAGGGGATGAGATGG+AGG - Chr4:77219588-77219607 MsG0480022828.01.T01:CDS 60.0%
GGGCCGGAAACTGTGCAGGA+AGG + Chr4:77219498-77219517 MsG0480022829.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 77218797 77220964 77218797 ID=MsG0480022828.01;Name=MsG0480022828.01
Chr4 mRNA 77218797 77220964 77218797 ID=MsG0480022828.01.T01;Parent=MsG0480022828.01;Name=MsG0480022828.01.T01;_AED=0.04;_eAED=0.04;_QI=0|0.5|0.33|1|1|1|3|279|490
Chr4 exon 77218797 77219177 77218797 ID=MsG0480022828.01.T01:exon:4782;Parent=MsG0480022828.01.T01
Chr4 exon 77219275 77219670 77219275 ID=MsG0480022828.01.T01:exon:4781;Parent=MsG0480022828.01.T01
Chr4 exon 77219990 77220964 77219990 ID=MsG0480022828.01.T01:exon:4780;Parent=MsG0480022828.01.T01
Chr4 CDS 77219990 77220964 77219990 ID=MsG0480022828.01.T01:cds;Parent=MsG0480022828.01.T01
Chr4 CDS 77219275 77219670 77219275 ID=MsG0480022828.01.T01:cds;Parent=MsG0480022828.01.T01
Chr4 CDS 77219076 77219177 77219076 ID=MsG0480022828.01.T01:cds;Parent=MsG0480022828.01.T01
Chr4 three_prime_UTR 77218797 77219075 77218797 ID=MsG0480022828.01.T01:three_prime_utr;Parent=MsG0480022828.01.T01
Gene Sequence

>MsG0480022828.01.T01

ATGAGTTCAGTTTCAGGAGACAACCAAAATATGCATGAGCAAACTGGTTGCTTTGATTCCGACACAATGGCAGAAGGTGTTGAAAACTCTCCAGAAGATAATAACTCTCCCCAAACCATGCCAAATCAAGTGGTTGCAGGTAACAGCAACAATAGCATTGAAGAGAATTTCAGGCCTTCTGTGGGAAGAGATTTTGATAAGGAGGAATTCTCTTATCACAATCATCACAACCCACAACACCTTGAAGATGTCTCCACTTATACAAATGGATTCACTCCCTCATCAGAAAACATTGCACAACAAAATCTTGGTTTGAACATAGGGAACTACTACTACAACAACATGGATAACATTTTGGAGCAAGAGGTGTATCAGAATTCGAGTTGGGACCCTAGTGCTCAGGATATGGACTATGCCAATCACCAAGAATACCACCAACTACACAACCATAAACAAAGCTACAATCCCTCAACCATTCAAGACCCTCATTATCCTTCACCAGATGTACTAAATCTTCACCACTTTCCTAGATCCTCAGCTTCATCTATTCTCACAAATCCATCCACCATTTGCATCACAACCCCAACTCAAAAGCCACCTAATTTCCATTACTCGATGAGTTTTCTTGGAGACCTTCCAATAGGCTCAGACAACTCAAGTGGATCATCAGTTTCATATGATCCTCTATTTCCTATGAACCTTCCTGCACAGTTTCCGGCCCTTAGAGAATTGCCTCAATCTCTCCCTCACGTCTATAGCATGCCTACCAACTCAAGGAATGGTTCTCGTTTTGGTGGAGGGGATGAGATGGAGGGAGATGGAGGTATGGGAGTGTCGGAATTCAACAAGGTCACTGCTTTTGTTGGCAAAGGAAAAGGAAAAGCTACAGAACATTTGACAACGGAGAAACAAAGAAGAGAGCAATTGAAGGGAAGATACAAAATCTTGAGGAGTCTCATCCCAAATTCCACCAAAGATGACAGAGCATCAGTGGTCGGAGATGCTATTGAGTATTTAAGAGAGCTGATTAGAACAGTGAATGAGCTGAAACTATTGGTGGAGAAGAAAAGACACGAGATGGAGATCTGCAAGAGACGTAAAACTGAAGACTATGCTGCAGAAAGCTGTCACATGAAGCCTTTTGGTGATCCAGATGGAAGCATAAGGACCTCATGGCTGCAGAGGAAATCAAAAGACAGTGAGGTTGATGTTCGGATCATTGATGATGATGTTACGATCAAACTGTTTCAGAGAAAGAAGGTTAATTGCTTGCTGTTTGTTTCCAAGGTTTTGGAGGAACTTCAGTTGGAACTTAACCATGTTGCAGGTGGACATGTTGGTGAATATTGTAGTTTCCTGTTCAATAGCAAGGTAACTGAAGGTTCTTCAGTGCATGCAAGTGCTATAGCAAACAGGGTGATTGATGTTCTGGACACACAATATGCAGCAGTTGTCCCACATAATAGGATGTAG
Protein sequence

>MsG0480022828.01.T01

MSSVSGDNQNMHEQTGCFDSDTMAEGVENSPEDNNSPQTMPNQVVAGNSNNSIEENFRPSVGRDFDKEEFSYHNHHNPQHLEDVSTYTNGFTPSSENIAQQNLGLNIGNYYYNNMDNILEQEVYQNSSWDPSAQDMDYANHQEYHQLHNHKQSYNPSTIQDPHYPSPDVLNLHHFPRSSASSILTNPSTICITTPTQKPPNFHYSMSFLGDLPIGSDNSSGSSVSYDPLFPMNLPAQFPALRELPQSLPHVYSMPTNSRNGSRFGGGDEMEGDGGMGVSEFNKVTAFVGKGKGKATEHLTTEKQRREQLKGRYKILRSLIPNSTKDDRASVVGDAIEYLRELIRTVNELKLLVEKKRHEMEICKRRKTEDYAAESCHMKPFGDPDGSIRTSWLQRKSKDSEVDVRIIDDDVTIKLFQRKKVNCLLFVSKVLEELQLELNHVAGGHVGEYCSFLFNSKVTEGSSVHASAIANRVIDVLDTQYAAVVPHNRM*