Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023409.01.T01 | XP_019414701.1 | 72.222 | 90 | 25 | 0 | 1 | 90 | 174 | 263 | 2.60E-34 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023409.01.T01 | Q9S840 | 72.222 | 72 | 20 | 0 | 1 | 72 | 174 | 245 | 1.35E-29 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023409.01.T01 | A0A2Z6MD95 | 75.581 | 86 | 20 | 1 | 1 | 85 | 149 | 234 | 1.29e-34 | 132 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0480023409.01.T01 | TF | SBP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023409.01.T01 | MTR_8g080680 | 74.118 | 85 | 22 | 0 | 1 | 85 | 137 | 221 | 3.21e-36 | 126 |
| MsG0480023409.01.T01 | MTR_3g085180 | 69.412 | 85 | 26 | 0 | 1 | 85 | 170 | 254 | 3.68e-35 | 124 |
| MsG0480023409.01.T01 | MTR_8g080690 | 72.973 | 74 | 20 | 0 | 1 | 74 | 156 | 229 | 4.96e-32 | 115 |
| MsG0480023409.01.T01 | MTR_8g463140 | 60.000 | 80 | 32 | 0 | 1 | 80 | 70 | 149 | 3.07e-28 | 100 |
| MsG0480023409.01.T01 | MTR_8g080670 | 68.571 | 70 | 22 | 0 | 1 | 70 | 167 | 236 | 1.07e-26 | 101 |
| MsG0480023409.01.T01 | MTR_4g088555 | 63.768 | 69 | 25 | 0 | 1 | 69 | 68 | 136 | 4.18e-25 | 92.0 |
| MsG0480023409.01.T01 | MTR_1g053715 | 54.348 | 92 | 41 | 1 | 1 | 91 | 79 | 170 | 5.29e-25 | 96.3 |
| MsG0480023409.01.T01 | MTR_2g078770 | 62.319 | 69 | 26 | 0 | 1 | 69 | 63 | 131 | 6.60e-25 | 92.4 |
| MsG0480023409.01.T01 | MTR_7g092930 | 53.846 | 91 | 41 | 1 | 1 | 90 | 73 | 163 | 8.15e-25 | 95.5 |
| MsG0480023409.01.T01 | MTR_5g046670 | 47.959 | 98 | 51 | 0 | 1 | 98 | 249 | 346 | 1.12e-24 | 96.7 |
| MsG0480023409.01.T01 | MTR_3g099080 | 57.333 | 75 | 32 | 0 | 1 | 75 | 103 | 177 | 2.40e-24 | 94.7 |
| MsG0480023409.01.T01 | MTR_7g444860 | 52.083 | 96 | 40 | 1 | 1 | 96 | 154 | 243 | 3.70e-24 | 93.6 |
| MsG0480023409.01.T01 | MTR_1g035010 | 50.000 | 100 | 49 | 1 | 1 | 99 | 109 | 208 | 4.04e-24 | 95.1 |
| MsG0480023409.01.T01 | MTR_8g096780 | 55.952 | 84 | 37 | 0 | 1 | 84 | 104 | 187 | 9.18e-24 | 93.2 |
| MsG0480023409.01.T01 | MTR_2g046550 | 48.980 | 98 | 48 | 1 | 1 | 98 | 144 | 239 | 1.06e-23 | 94.0 |
| MsG0480023409.01.T01 | MTR_4g109770 | 50.000 | 92 | 44 | 1 | 1 | 92 | 128 | 217 | 1.22e-23 | 93.6 |
| MsG0480023409.01.T01 | MTR_7g028740 | 54.667 | 75 | 34 | 0 | 1 | 75 | 82 | 156 | 2.08e-22 | 89.4 |
| MsG0480023409.01.T01 | MTR_2g461920 | 59.420 | 69 | 28 | 0 | 1 | 69 | 176 | 244 | 3.02e-22 | 89.7 |
| MsG0480023409.01.T01 | MTR_8g005960 | 53.333 | 90 | 39 | 2 | 1 | 87 | 192 | 281 | 5.74e-22 | 87.8 |
| MsG0480023409.01.T01 | MTR_1g086250 | 56.000 | 75 | 33 | 0 | 1 | 75 | 152 | 226 | 8.90e-22 | 88.6 |
| MsG0480023409.01.T01 | MTR_2g014200 | 56.522 | 69 | 30 | 0 | 1 | 69 | 69 | 137 | 1.03e-21 | 83.2 |
| MsG0480023409.01.T01 | MTR_7g110320 | 53.086 | 81 | 38 | 0 | 1 | 81 | 150 | 230 | 1.33e-21 | 88.2 |
| MsG0480023409.01.T01 | MTR_7g110320 | 53.750 | 80 | 37 | 0 | 1 | 80 | 150 | 229 | 1.55e-21 | 87.8 |
| MsG0480023409.01.T01 | MTR_2g020620 | 41.758 | 91 | 53 | 0 | 3 | 93 | 97 | 187 | 4.13e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023409.01.T01 | AT5G43270 | 72.222 | 72 | 20 | 0 | 1 | 72 | 174 | 245 | 1.38e-30 | 112 |
| MsG0480023409.01.T01 | AT5G43270 | 72.222 | 72 | 20 | 0 | 1 | 72 | 174 | 245 | 1.38e-30 | 112 |
| MsG0480023409.01.T01 | AT5G43270 | 72.222 | 72 | 20 | 0 | 1 | 72 | 174 | 245 | 1.38e-30 | 112 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 2.51e-28 | 105 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 5.51e-28 | 104 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 5.51e-28 | 104 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 5.51e-28 | 104 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 5.51e-28 | 104 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 5.51e-28 | 104 |
| MsG0480023409.01.T01 | AT1G27370 | 66.667 | 75 | 23 | 1 | 1 | 75 | 181 | 253 | 5.51e-28 | 104 |
| MsG0480023409.01.T01 | AT1G27360 | 64.000 | 75 | 25 | 1 | 1 | 75 | 180 | 252 | 7.00e-26 | 99.4 |
| MsG0480023409.01.T01 | AT1G27360 | 64.000 | 75 | 25 | 1 | 1 | 75 | 180 | 252 | 7.00e-26 | 99.4 |
| MsG0480023409.01.T01 | AT1G27360 | 64.000 | 75 | 25 | 1 | 1 | 75 | 180 | 252 | 7.00e-26 | 99.4 |
| MsG0480023409.01.T01 | AT1G27360 | 64.000 | 75 | 25 | 1 | 1 | 75 | 180 | 252 | 7.00e-26 | 99.4 |
| MsG0480023409.01.T01 | AT1G76580 | 48.000 | 100 | 50 | 1 | 1 | 98 | 119 | 218 | 7.93e-25 | 97.4 |
| MsG0480023409.01.T01 | AT1G76580 | 48.000 | 100 | 50 | 1 | 1 | 98 | 119 | 218 | 8.49e-25 | 97.1 |
| MsG0480023409.01.T01 | AT2G42200 | 58.667 | 75 | 31 | 0 | 1 | 75 | 79 | 153 | 8.92e-25 | 95.9 |
| MsG0480023409.01.T01 | AT1G76580 | 48.000 | 100 | 50 | 1 | 1 | 98 | 119 | 218 | 9.03e-25 | 97.1 |
| MsG0480023409.01.T01 | AT3G60030 | 46.939 | 98 | 52 | 0 | 1 | 98 | 132 | 229 | 2.31e-24 | 95.9 |
| MsG0480023409.01.T01 | AT2G47070 | 58.904 | 73 | 30 | 0 | 1 | 73 | 111 | 183 | 3.82e-23 | 92.4 |
| MsG0480023409.01.T01 | AT1G53160 | 53.086 | 81 | 38 | 0 | 1 | 81 | 59 | 139 | 5.17e-23 | 87.4 |
| MsG0480023409.01.T01 | AT1G53160 | 53.086 | 81 | 38 | 0 | 1 | 81 | 59 | 139 | 6.74e-23 | 87.4 |
| MsG0480023409.01.T01 | AT1G53160 | 53.086 | 81 | 38 | 0 | 1 | 81 | 59 | 139 | 6.74e-23 | 87.4 |
| MsG0480023409.01.T01 | AT2G33810 | 57.971 | 69 | 29 | 0 | 1 | 69 | 59 | 127 | 1.13e-22 | 85.5 |
| MsG0480023409.01.T01 | AT3G15270 | 55.405 | 74 | 33 | 0 | 1 | 74 | 68 | 141 | 1.70e-22 | 86.3 |
| MsG0480023409.01.T01 | AT1G69170 | 60.870 | 69 | 27 | 0 | 1 | 69 | 129 | 197 | 4.03e-22 | 89.0 |
| MsG0480023409.01.T01 | AT5G50670 | 54.667 | 75 | 34 | 0 | 1 | 75 | 106 | 180 | 1.56e-21 | 87.0 |
| MsG0480023409.01.T01 | AT5G50670 | 54.667 | 75 | 34 | 0 | 1 | 75 | 106 | 180 | 1.56e-21 | 87.0 |
| MsG0480023409.01.T01 | AT5G50570 | 54.667 | 75 | 34 | 0 | 1 | 75 | 106 | 180 | 1.56e-21 | 87.0 |
| MsG0480023409.01.T01 | AT5G50570 | 54.667 | 75 | 34 | 0 | 1 | 75 | 106 | 180 | 1.56e-21 | 87.0 |
| MsG0480023409.01.T01 | AT1G20980 | 46.602 | 103 | 50 | 2 | 1 | 98 | 125 | 227 | 1.59e-21 | 87.8 |
| MsG0480023409.01.T01 | AT1G02065 | 52.747 | 91 | 38 | 1 | 1 | 91 | 193 | 278 | 2.58e-21 | 86.3 |
| MsG0480023409.01.T01 | AT3G57920 | 45.098 | 102 | 53 | 2 | 1 | 99 | 64 | 165 | 8.35e-20 | 82.4 |
| MsG0480023409.01.T01 | AT2G47070 | 58.491 | 53 | 22 | 0 | 21 | 73 | 2 | 54 | 8.98e-15 | 68.6 |
| MsG0480023409.01.T01 | AT5G18830 | 47.826 | 69 | 36 | 0 | 1 | 69 | 143 | 211 | 1.06e-14 | 68.6 |
| MsG0480023409.01.T01 | AT5G18830 | 47.826 | 69 | 36 | 0 | 1 | 69 | 143 | 211 | 1.10e-14 | 68.6 |
| MsG0480023409.01.T01 | AT5G18830 | 47.826 | 69 | 36 | 0 | 1 | 69 | 143 | 211 | 1.12e-14 | 68.6 |
Find 17 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGCTTGTTATCTATTAT+TGG | 0.124259 | 4:-84821135 | MsG0480023409.01.T01:CDS |
| TGACTTGATTGGACAGATTC+AGG | 0.307609 | 4:+84821195 | None:intergenic |
| CAGATTCAGGATGATATTTA+CGG | 0.380220 | 4:+84821208 | None:intergenic |
| ACAAAGCTGATTGACTTGAT+TGG | 0.380359 | 4:+84821184 | None:intergenic |
| AATAGATAACAAGCAGAAAC+TGG | 0.401337 | 4:+84821140 | None:intergenic |
| CATCGAACTCAAACAGATCA+TGG | 0.413681 | 4:+84821280 | None:intergenic |
| ACACCAGCTATAACCACCTT+TGG | 0.433788 | 4:+84821513 | None:intergenic |
| ACGTATGCGATGATAATCTT+TGG | 0.442176 | 4:+84821563 | None:intergenic |
| GTGTGACCTTGACCTTTCAT+TGG | 0.491318 | 4:-84821586 | MsG0480023409.01.T01:CDS |
| TATCCAAAGGTGGTTATAGC+TGG | 0.492634 | 4:-84821516 | MsG0480023409.01.T01:CDS |
| GCTTTGTCTTCTTCATCCTG+TGG | 0.538137 | 4:-84821168 | MsG0480023409.01.T01:CDS |
| GATAATCTTTGGCCAATGAA+AGG | 0.562156 | 4:+84821574 | None:intergenic |
| AAAGGTGGTTATAGCTGGTG+TGG | 0.575359 | 4:-84821511 | MsG0480023409.01.T01:CDS |
| GCAGAAACTGGTATTACCAC+AGG | 0.602886 | 4:+84821152 | None:intergenic |
| CTTTGGCCAATGAAAGGTCA+AGG | 0.612836 | 4:+84821580 | None:intergenic |
| AAGTCATTCAAAATATCCAA+AGG | 0.642743 | 4:-84821529 | MsG0480023409.01.T01:CDS |
| TCATTCAAAATATCCAAAGG+TGG | 0.681356 | 4:-84821526 | MsG0480023409.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATAGTATTTTTTTTATGA+TGG | - | Chr4:84821305-84821324 | MsG0480023409.01.T01:intron | 10.0% |
| !! | TGTTTAGATATAATTGATAT+AGG | - | Chr4:84821279-84821298 | MsG0480023409.01.T01:CDS | 15.0% |
| ! | AAGTCATTCAAAATATCCAA+AGG | - | Chr4:84821184-84821203 | MsG0480023409.01.T01:CDS | 25.0% |
| !!! | TTCTGCTTGTTATCTATTAT+TGG | - | Chr4:84821578-84821597 | MsG0480023409.01.T01:CDS | 25.0% |
| AATAGATAACAAGCAGAAAC+TGG | + | Chr4:84821576-84821595 | None:intergenic | 30.0% | |
| CAGATTCAGGATGATATTTA+CGG | + | Chr4:84821508-84821527 | None:intergenic | 30.0% | |
| TCATTCAAAATATCCAAAGG+TGG | - | Chr4:84821187-84821206 | MsG0480023409.01.T01:CDS | 30.0% | |
| ACGTATGCGATGATAATCTT+TGG | + | Chr4:84821153-84821172 | None:intergenic | 35.0% | |
| GATAATCTTTGGCCAATGAA+AGG | + | Chr4:84821142-84821161 | None:intergenic | 35.0% | |
| ! | AGCTTTACCTGCTACATTTT+TGG | + | Chr4:84821241-84821260 | None:intergenic | 35.0% |
| ! | GATTTTGCCAAAAATGTAGC+AGG | - | Chr4:84821231-84821250 | MsG0480023409.01.T01:CDS | 35.0% |
| !! | ACAAAGCTGATTGACTTGAT+TGG | + | Chr4:84821532-84821551 | None:intergenic | 35.0% |
| !!! | AACAAACTTGCTGTCTTTTC+TGG | - | Chr4:84821374-84821393 | MsG0480023409.01.T01:intron | 35.0% |
| CATCGAACTCAAACAGATCA+TGG | + | Chr4:84821436-84821455 | None:intergenic | 40.0% | |
| TATCCAAAGGTGGTTATAGC+TGG | - | Chr4:84821197-84821216 | MsG0480023409.01.T01:CDS | 40.0% | |
| TGACTTGATTGGACAGATTC+AGG | + | Chr4:84821521-84821540 | None:intergenic | 40.0% | |
| !!! | AACTTGCTGTCTTTTCTGGA+TGG | - | Chr4:84821378-84821397 | MsG0480023409.01.T01:intron | 40.0% |
| AAAGGTGGTTATAGCTGGTG+TGG | - | Chr4:84821202-84821221 | MsG0480023409.01.T01:CDS | 45.0% | |
| ACACCAGCTATAACCACCTT+TGG | + | Chr4:84821203-84821222 | None:intergenic | 45.0% | |
| CTTTGGCCAATGAAAGGTCA+AGG | + | Chr4:84821136-84821155 | None:intergenic | 45.0% | |
| GCAGAAACTGGTATTACCAC+AGG | + | Chr4:84821564-84821583 | None:intergenic | 45.0% | |
| GCTTTGTCTTCTTCATCCTG+TGG | - | Chr4:84821545-84821564 | MsG0480023409.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 84821128 | 84821607 | 84821128 | ID=MsG0480023409.01;Name=MsG0480023409.01 |
| Chr4 | mRNA | 84821128 | 84821607 | 84821128 | ID=MsG0480023409.01.T01;Parent=MsG0480023409.01;Name=MsG0480023409.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0|0|2|0|100 |
| Chr4 | exon | 84821128 | 84821305 | 84821128 | ID=MsG0480023409.01.T01:exon:5731;Parent=MsG0480023409.01.T01 |
| Chr4 | exon | 84821483 | 84821607 | 84821483 | ID=MsG0480023409.01.T01:exon:5730;Parent=MsG0480023409.01.T01 |
| Chr4 | CDS | 84821483 | 84821607 | 84821483 | ID=MsG0480023409.01.T01:cds;Parent=MsG0480023409.01.T01 |
| Chr4 | CDS | 84821128 | 84821305 | 84821128 | ID=MsG0480023409.01.T01:cds;Parent=MsG0480023409.01.T01 |
| Gene Sequence |
| Protein sequence |
