AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023446.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023446.01.T01 MTR_8g093070 97.082 377 11 0 1 377 131 507 0.0 759
MsG0480023446.01.T01 MTR_3g072710 38.095 399 194 12 6 366 117 500 2.66e-67 221
MsG0480023446.01.T01 MTR_5g058860 38.619 391 201 14 6 366 116 497 2.06e-65 216
MsG0480023446.01.T01 MTR_3g065980 32.905 389 214 15 6 365 169 539 1.94e-40 150
MsG0480023446.01.T01 MTR_7g027190 32.242 397 211 16 6 365 297 672 3.00e-39 148
MsG0480023446.01.T01 MTR_4g076020 31.081 370 215 11 14 369 89 432 1.80e-37 140
MsG0480023446.01.T01 MTR_4g102790 29.592 392 233 15 6 369 289 665 3.47e-37 142
MsG0480023446.01.T01 MTR_7g074650 27.614 373 229 14 14 367 443 793 1.96e-33 132
MsG0480023446.01.T01 MTR_4g077760 32.353 374 214 17 14 365 198 554 1.42e-31 125
MsG0480023446.01.T01 MTR_1g096030 30.633 395 226 18 6 365 77 458 1.92e-27 113
MsG0480023446.01.T01 MTR_2g082090 27.604 384 240 16 6 365 209 578 5.04e-26 110
MsG0480023446.01.T01 MTR_1g069725 26.302 384 236 13 5 366 105 463 9.14e-26 108
MsG0480023446.01.T01 MTR_4g133660 27.747 364 221 15 22 366 203 543 1.58e-25 108
MsG0480023446.01.T01 MTR_0092s0100 26.542 373 253 9 3 365 366 727 1.78e-25 108
MsG0480023446.01.T01 MTR_0092s0100 26.542 373 253 9 3 365 369 730 1.87e-25 108
MsG0480023446.01.T01 MTR_8g442410 26.823 384 246 13 6 365 152 524 3.47e-25 107
MsG0480023446.01.T01 MTR_2g034260 26.396 394 247 14 2 365 203 583 1.53e-24 105
MsG0480023446.01.T01 MTR_5g094450 28.435 313 197 12 71 365 228 531 2.55e-24 104
MsG0480023446.01.T01 MTR_4g064150 25.935 401 244 17 2 368 354 735 3.83e-24 104
MsG0480023446.01.T01 MTR_5g015490 26.684 386 250 16 8 367 113 491 4.44e-24 103
MsG0480023446.01.T01 MTR_2g034250 27.318 399 238 16 2 365 203 584 5.08e-24 103
MsG0480023446.01.T01 MTR_3g089055 26.649 379 244 12 9 365 198 564 1.21e-23 102
MsG0480023446.01.T01 MTR_3g056110 29.032 310 194 11 73 365 241 541 1.30e-23 102
MsG0480023446.01.T01 MTR_3g089055 26.649 379 244 12 9 365 203 569 1.41e-23 102
MsG0480023446.01.T01 MTR_5g097480 30.065 306 180 12 81 365 251 543 1.67e-23 102
MsG0480023446.01.T01 MTR_1g029420 27.368 380 240 14 9 365 225 591 2.08e-23 102
MsG0480023446.01.T01 MTR_4g064200 25.648 386 244 15 9 368 284 652 3.48e-23 101
MsG0480023446.01.T01 MTR_2g026250 26.257 358 220 15 38 365 252 595 9.09e-23 100
MsG0480023446.01.T01 MTR_7g062120 25.316 395 250 16 2 368 365 742 1.03e-22 100
MsG0480023446.01.T01 MTR_2g097473 24.339 378 248 15 14 368 294 656 1.22e-22 100
MsG0480023446.01.T01 MTR_2g097410 24.682 393 257 16 2 368 364 743 1.49e-22 100
MsG0480023446.01.T01 MTR_2g034280 27.136 398 237 17 2 365 196 574 1.90e-22 99.4
MsG0480023446.01.T01 MTR_2g097467 24.468 376 248 16 14 367 294 655 2.88e-22 99.0
MsG0480023446.01.T01 MTR_4g097080 26.166 386 246 14 8 367 132 504 3.03e-22 98.6
MsG0480023446.01.T01 MTR_4g104020 27.577 388 239 14 6 365 146 519 8.97e-22 97.1
MsG0480023446.01.T01 MTR_2g097463 24.227 388 259 16 4 368 282 657 1.73e-21 96.7
MsG0480023446.01.T01 MTR_2g089100 23.892 406 253 14 9 369 52 446 1.12e-19 90.5
MsG0480023446.01.T01 MTR_4g064120 24.495 396 248 16 6 368 251 628 1.73e-19 90.5
MsG0480023446.01.T01 MTR_4g026485 26.702 382 240 16 3 365 263 623 1.00e-18 88.2
MsG0480023446.01.T01 MTR_4g064180 25.076 331 205 13 6 310 251 564 1.14e-17 85.1
MsG0480023446.01.T01 MTR_4g064160 25.381 394 245 17 5 368 312 686 1.68e-17 84.7
MsG0480023446.01.T01 MTR_8g077940 27.078 373 246 13 7 365 180 540 2.05e-17 84.0
MsG0480023446.01.T01 MTR_2g097310 23.834 386 265 15 2 368 265 640 4.82e-17 83.2
MsG0480023446.01.T01 MTR_2g097350 23.514 387 265 13 2 368 267 642 5.10e-17 83.2
MsG0480023446.01.T01 MTR_2g097390 23.057 386 268 14 2 368 268 643 1.40e-15 78.6
MsG0480023446.01.T01 MTR_7g109580 28.523 298 174 14 89 365 274 553 1.93e-15 78.2
MsG0480023446.01.T01 MTR_5g015950 22.877 424 253 12 9 368 35 448 2.01e-15 77.8
MsG0480023446.01.T01 MTR_3g053270 22.877 424 253 12 9 368 35 448 2.01e-15 77.8
MsG0480023446.01.T01 MTR_2g099110 24.444 360 216 14 47 367 110 452 2.12e-14 74.7
MsG0480023446.01.T01 MTR_5g009080 29.834 181 116 7 6 180 53 228 4.11e-14 73.9
MsG0480023446.01.T01 MTR_1g086970 24.937 397 247 17 2 365 99 477 4.45e-14 73.9
MsG0480023446.01.T01 MTR_4g095500 22.565 421 254 14 9 367 59 469 3.87e-13 70.9
MsG0480023446.01.T01 MTR_4g076140 28.155 206 134 7 6 202 45 245 1.26e-12 69.3
MsG0480023446.01.T01 MTR_2g097380 23.301 309 203 11 73 368 276 563 1.79e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023446.01.T01 AT4G08250 32.905 389 228 14 6 371 104 482 1.76e-48 171
MsG0480023446.01.T01 AT1G55580 35.238 315 162 12 79 365 144 444 1.29e-43 157
MsG0480023446.01.T01 AT5G41920 32.275 378 220 14 6 365 40 399 1.29e-41 151
MsG0480023446.01.T01 AT3G03450 29.708 377 236 13 6 365 180 544 6.20e-38 143
MsG0480023446.01.T01 AT1G66350 29.443 377 226 14 6 365 152 505 2.39e-36 138
MsG0480023446.01.T01 AT5G17490 30.130 385 230 14 1 365 150 515 6.07e-35 135
MsG0480023446.01.T01 AT2G01570 29.351 385 222 16 6 365 221 580 2.17e-33 131
MsG0480023446.01.T01 AT1G14920 28.389 391 218 17 6 365 169 528 3.35e-32 127
MsG0480023446.01.T01 AT3G54220 27.513 378 226 14 14 367 298 651 1.31e-31 126
MsG0480023446.01.T01 AT5G66770 29.738 343 203 12 47 365 255 583 1.03e-30 123
MsG0480023446.01.T01 AT1G63100 25.564 399 241 16 6 366 249 629 1.79e-30 123
MsG0480023446.01.T01 AT1G63100 25.823 395 245 15 6 366 274 654 2.46e-30 122
MsG0480023446.01.T01 AT5G59450 27.561 410 242 19 3 376 218 608 6.92e-30 121
MsG0480023446.01.T01 AT3G50650 29.661 354 211 14 37 365 201 541 2.03e-29 119
MsG0480023446.01.T01 AT4G17230 29.041 365 220 14 27 368 179 527 5.70e-29 118
MsG0480023446.01.T01 AT4G17230 29.041 365 220 14 27 368 179 527 5.70e-29 118
MsG0480023446.01.T01 AT2G04890 28.307 378 232 16 9 365 53 412 1.01e-27 113
MsG0480023446.01.T01 AT3G46600 28.205 390 234 15 6 367 1 372 1.27e-27 112
MsG0480023446.01.T01 AT3G46600 27.665 394 239 15 2 367 173 548 5.52e-27 112
MsG0480023446.01.T01 AT3G46600 27.949 390 235 15 6 367 209 580 9.23e-27 112
MsG0480023446.01.T01 AT1G50600 26.415 371 245 13 14 365 239 600 2.38e-26 110
MsG0480023446.01.T01 AT1G50600 26.415 371 245 13 14 365 164 525 2.45e-26 110
MsG0480023446.01.T01 AT1G50600 26.415 371 245 13 14 365 235 596 3.30e-26 110
MsG0480023446.01.T01 AT5G48150 28.535 396 235 16 1 365 111 489 3.87e-26 109
MsG0480023446.01.T01 AT5G48150 28.535 396 235 16 1 365 111 489 3.87e-26 109
MsG0480023446.01.T01 AT5G48150 28.535 396 235 16 1 365 111 489 3.87e-26 109
MsG0480023446.01.T01 AT5G48150 28.535 396 235 16 1 365 111 489 3.87e-26 109
MsG0480023446.01.T01 AT1G07530 27.318 399 238 17 2 368 389 767 5.26e-25 107
MsG0480023446.01.T01 AT4G37650 26.786 392 249 14 9 368 146 531 8.98e-25 106
MsG0480023446.01.T01 AT1G21450 29.565 345 204 15 47 365 261 592 4.24e-24 104
MsG0480023446.01.T01 AT2G29060 26.750 400 239 19 2 367 314 693 9.66e-23 100
MsG0480023446.01.T01 AT2G29060 26.500 400 240 19 2 367 277 656 1.07e-22 100
MsG0480023446.01.T01 AT2G37650 24.548 387 259 12 2 367 328 702 2.40e-21 96.3
MsG0480023446.01.T01 AT2G37650 24.548 387 259 12 2 367 340 714 2.73e-21 96.3
MsG0480023446.01.T01 AT4G00150 27.851 377 229 17 5 365 204 553 1.01e-20 94.4
MsG0480023446.01.T01 AT1G07520 23.907 389 256 14 9 368 154 531 2.99e-18 86.7
MsG0480023446.01.T01 AT1G07520 23.907 389 256 14 9 368 154 531 2.99e-18 86.7
MsG0480023446.01.T01 AT1G07520 23.907 389 256 14 9 368 319 696 4.16e-18 86.7
MsG0480023446.01.T01 AT1G07520 23.907 389 256 14 9 368 318 695 4.39e-18 86.7
MsG0480023446.01.T01 AT1G50420 23.733 434 248 16 6 365 54 478 8.21e-18 85.1
MsG0480023446.01.T01 AT4G36710 27.100 369 249 14 8 365 125 484 1.17e-17 84.7
MsG0480023446.01.T01 AT5G52510 26.984 315 190 11 82 365 334 639 2.17e-17 84.3
MsG0480023446.01.T01 AT2G29065 25.309 324 198 13 73 368 323 630 5.42e-17 83.2
MsG0480023446.01.T01 AT3G49950 23.846 390 256 12 9 367 30 409 3.90e-16 79.7
MsG0480023446.01.T01 AT3G60630 24.451 319 184 12 79 365 328 621 1.03e-13 73.2

Find 50 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACCTCTTTCTCTATA+AGG 0.158083 4:-85232810 None:intergenic
CTAAACAATGCCTCGTCTTT+AGG 0.252519 4:+85232070 MsG0480023446.01.T01:CDS
CTGTGATTGCTGTTACTCTA+AGG 0.272598 4:-85232396 None:intergenic
GCTTGTTTGGTAAAGGGTAT+TGG 0.274576 4:+85232980 MsG0480023446.01.T01:CDS
AAGCTATTGGTTAGCTTGTT+TGG 0.278423 4:+85232967 MsG0480023446.01.T01:CDS
AACAAGCCTTGGTGATGATT+TGG 0.290948 4:-85232641 None:intergenic
CTTTGACTTCTGTTTCAATC+TGG 0.297827 4:+85233049 MsG0480023446.01.T01:CDS
CTTGTTTGGTAAAGGGTATT+GGG 0.313644 4:+85232981 MsG0480023446.01.T01:CDS
CAATACCTCTTTCTCTATAA+GGG 0.331359 4:-85232809 None:intergenic
TATTTCACAGGCTAAGCTAT+TGG 0.357597 4:+85232954 MsG0480023446.01.T01:CDS
TGGTTAGCTTGTTTGGTAAA+GGG 0.385802 4:+85232974 MsG0480023446.01.T01:CDS
TAAGGGCTAACTCCATCTTA+TGG 0.393772 4:-85232792 None:intergenic
AAGGCGATAGTTTATTGAAC+AGG 0.393824 4:+85232095 MsG0480023446.01.T01:CDS
CCTTATAGAGAAAGAGGTAT+TGG 0.394224 4:+85232810 MsG0480023446.01.T01:CDS
AATCCAATGGCCACCGTTGA+TGG 0.402840 4:+85232342 MsG0480023446.01.T01:CDS
TTGGTTAGCTTGTTTGGTAA+AGG 0.407587 4:+85232973 MsG0480023446.01.T01:CDS
ATCAATGAGAAACTCCTGTT+TGG 0.411022 4:-85232595 None:intergenic
ACTCAAATCCATGTCCTTTG+TGG 0.411956 4:+85232699 MsG0480023446.01.T01:CDS
TTTGACTTCTGTTTCAATCT+GGG 0.412837 4:+85233050 MsG0480023446.01.T01:CDS
TACATCAGTGGATGCCAAAC+AGG 0.416877 4:+85232581 MsG0480023446.01.T01:CDS
AGCTTCGAAAATCGCTTGAT+TGG 0.418613 4:-85232269 None:intergenic
CTTATAGAGAAAGAGGTATT+GGG 0.435957 4:+85232811 MsG0480023446.01.T01:CDS
CAACCATCAACGGTGGCCAT+TGG 0.475147 4:-85232345 None:intergenic
GTTAGCCCTTATAGAGAAAG+AGG 0.487244 4:+85232804 MsG0480023446.01.T01:CDS
AATGCCTCGTCTTTAGGAAA+AGG 0.493064 4:+85232076 MsG0480023446.01.T01:CDS
TCGACTAAAACAACAAGCCT+TGG 0.505560 4:-85232652 None:intergenic
ACATGTTCTGAATGGAACTT+AGG 0.509744 4:-85232166 None:intergenic
GCAATATGAGAACTGTAAGT+TGG 0.510443 4:+85233005 MsG0480023446.01.T01:CDS
AAAAGAGGAGAGAATATTGT+GGG 0.510893 4:+85232873 MsG0480023446.01.T01:CDS
GAAGCCTCAGTTCAACAAAC+AGG 0.521298 4:+85232433 MsG0480023446.01.T01:CDS
TTACTCATATTATAATCTGT+TGG 0.522232 4:-85233075 None:intergenic
ACTGTAAGTTGGCTTTGTGT+TGG 0.525441 4:+85233016 MsG0480023446.01.T01:CDS
GAATTCAAAGAAATTGAACT+TGG 0.527275 4:+85232532 MsG0480023446.01.T01:CDS
CACGTTCGATACAGTAATGA+TGG 0.531854 4:+85232498 MsG0480023446.01.T01:CDS
GTGTAGAAACATGTTCTGAA+TGG 0.541579 4:-85232174 None:intergenic
TCTGTGGAGTAGCCATAAGA+TGG 0.563300 4:+85232780 MsG0480023446.01.T01:CDS
TTGAAGGGGTATAGACGTGT+TGG 0.563890 4:+85232916 MsG0480023446.01.T01:CDS
TCTGCCTGTTTGTTGAACTG+AGG 0.584240 4:-85232437 None:intergenic
TGTGATTGCTGTTACTCTAA+GGG 0.585072 4:-85232395 None:intergenic
GAGTACATGCAATATTTCAC+AGG 0.587078 4:+85232942 MsG0480023446.01.T01:CDS
ATTACTGTATCGAACGTGAA+CGG 0.593883 4:-85232493 None:intergenic
CAGTTCAACAAACAGGCAGA+AGG 0.599250 4:+85232440 MsG0480023446.01.T01:CDS
ATTGCAAAGTCAACCATCAA+CGG 0.607337 4:-85232355 None:intergenic
CAGATTGAAACAGAAGTCAA+AGG 0.620209 4:-85233048 None:intergenic
ATATCATGGAGGGAATCCAA+TGG 0.669550 4:+85232329 MsG0480023446.01.T01:CDS
ATTCACTTGTAAGTACTCTG+TGG 0.679195 4:+85232764 MsG0480023446.01.T01:CDS
TCAATTGTATGATACATCAG+TGG 0.691913 4:+85232569 MsG0480023446.01.T01:CDS
GCAAAGTCAACCATCAACGG+TGG 0.711871 4:-85232352 None:intergenic
ACGTAACCAAATCATCACCA+AGG 0.735440 4:+85232635 MsG0480023446.01.T01:CDS
TAGAGTAACAGCAATCACAG+TGG 0.741841 4:+85232399 MsG0480023446.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTTTTATTCATTGA+AGG + Chr4:85232900-85232919 MsG0480023446.01.T01:CDS 15.0%
!!! AGATTTTTTTATTCATTGAA+GGG + Chr4:85232901-85232920 MsG0480023446.01.T01:CDS 15.0%
!! TTACTCATATTATAATCTGT+TGG - Chr4:85233078-85233097 None:intergenic 20.0%
!!! AAAATGAGCAAATTTTACAT+AGG - Chr4:85232245-85232264 None:intergenic 20.0%
!!! AAATGAGCAAATTTTACATA+GGG - Chr4:85232244-85232263 None:intergenic 20.0%
!!! GATTTTTTTATTCATTGAAG+GGG + Chr4:85232902-85232921 MsG0480023446.01.T01:CDS 20.0%
! GAATTCAAAGAAATTGAACT+TGG + Chr4:85232532-85232551 MsG0480023446.01.T01:CDS 25.0%
!!! ATTGATTTTGATATCATGGA+GGG + Chr4:85232319-85232338 MsG0480023446.01.T01:CDS 25.0%
!!! TGTCATTGATTTTGATATCA+TGG + Chr4:85232315-85232334 MsG0480023446.01.T01:CDS 25.0%
AAAAAGAGGAGAGAATATTG+TGG + Chr4:85232872-85232891 MsG0480023446.01.T01:CDS 30.0%
AAAAGAGGAGAGAATATTGT+GGG + Chr4:85232873-85232892 MsG0480023446.01.T01:CDS 30.0%
CAATACCTCTTTCTCTATAA+GGG - Chr4:85232812-85232831 None:intergenic 30.0%
TCAATTGTATGATACATCAG+TGG + Chr4:85232569-85232588 MsG0480023446.01.T01:CDS 30.0%
! TTAGTTGATCTTTTGCTGAT+AGG + Chr4:85231992-85232011 MsG0480023446.01.T01:CDS 30.0%
! TTTGACTTCTGTTTCAATCT+GGG + Chr4:85233050-85233069 MsG0480023446.01.T01:CDS 30.0%
!! CTTATAGAGAAAGAGGTATT+GGG + Chr4:85232811-85232830 MsG0480023446.01.T01:CDS 30.0%
!!! CATTGATTTTGATATCATGG+AGG + Chr4:85232318-85232337 MsG0480023446.01.T01:CDS 30.0%
AAGGCGATAGTTTATTGAAC+AGG + Chr4:85232095-85232114 MsG0480023446.01.T01:CDS 35.0%
ACAGTTTCCTTGTGAAAAAG+AGG + Chr4:85232858-85232877 MsG0480023446.01.T01:CDS 35.0%
ATCAATGAGAAACTCCTGTT+TGG - Chr4:85232598-85232617 None:intergenic 35.0%
ATTACTGTATCGAACGTGAA+CGG - Chr4:85232496-85232515 None:intergenic 35.0%
ATTCACTTGTAAGTACTCTG+TGG + Chr4:85232764-85232783 MsG0480023446.01.T01:CDS 35.0%
ATTGCAAAGTCAACCATCAA+CGG - Chr4:85232358-85232377 None:intergenic 35.0%
CAGATTGAAACAGAAGTCAA+AGG - Chr4:85233051-85233070 None:intergenic 35.0%
CCAATACCTCTTTCTCTATA+AGG - Chr4:85232813-85232832 None:intergenic 35.0%
GAGTACATGCAATATTTCAC+AGG + Chr4:85232942-85232961 MsG0480023446.01.T01:CDS 35.0%
GCAATATGAGAACTGTAAGT+TGG + Chr4:85233005-85233024 MsG0480023446.01.T01:CDS 35.0%
TATTTCACAGGCTAAGCTAT+TGG + Chr4:85232954-85232973 MsG0480023446.01.T01:CDS 35.0%
! ACATGTTCTGAATGGAACTT+AGG - Chr4:85232169-85232188 None:intergenic 35.0%
! CCTTATAGAGAAAGAGGTAT+TGG + Chr4:85232810-85232829 MsG0480023446.01.T01:CDS 35.0%
! CTTTGACTTCTGTTTCAATC+TGG + Chr4:85233049-85233068 MsG0480023446.01.T01:CDS 35.0%
! GTGTAGAAACATGTTCTGAA+TGG - Chr4:85232177-85232196 None:intergenic 35.0%
! TGTGATTGCTGTTACTCTAA+GGG - Chr4:85232398-85232417 None:intergenic 35.0%
! TTGTGTCTTTTCTTTACGCA+AGG + Chr4:85232118-85232137 MsG0480023446.01.T01:CDS 35.0%
!! AAGCTATTGGTTAGCTTGTT+TGG + Chr4:85232967-85232986 MsG0480023446.01.T01:CDS 35.0%
!! ATTCTCTCCTCTTTTTCACA+AGG - Chr4:85232868-85232887 None:intergenic 35.0%
!! CTTGTTTGGTAAAGGGTATT+GGG + Chr4:85232981-85233000 MsG0480023446.01.T01:CDS 35.0%
!! TGGTTAGCTTGTTTGGTAAA+GGG + Chr4:85232974-85232993 MsG0480023446.01.T01:CDS 35.0%
!! TTGGTTAGCTTGTTTGGTAA+AGG + Chr4:85232973-85232992 MsG0480023446.01.T01:CDS 35.0%
ACGTAACCAAATCATCACCA+AGG + Chr4:85232635-85232654 MsG0480023446.01.T01:CDS 40.0%
ACTCAAATCCATGTCCTTTG+TGG + Chr4:85232699-85232718 MsG0480023446.01.T01:CDS 40.0%
AGCTTCGAAAATCGCTTGAT+TGG - Chr4:85232272-85232291 None:intergenic 40.0%
ATATCATGGAGGGAATCCAA+TGG + Chr4:85232329-85232348 MsG0480023446.01.T01:CDS 40.0%
CACGTTCGATACAGTAATGA+TGG + Chr4:85232498-85232517 MsG0480023446.01.T01:CDS 40.0%
CTAAACAATGCCTCGTCTTT+AGG + Chr4:85232070-85232089 MsG0480023446.01.T01:CDS 40.0%
CTCACAGAAAAACTCCACAA+AGG - Chr4:85232716-85232735 None:intergenic 40.0%
GAAAAACTCCACAAAGGACA+TGG - Chr4:85232710-85232729 None:intergenic 40.0%
GTTAGCCCTTATAGAGAAAG+AGG + Chr4:85232804-85232823 MsG0480023446.01.T01:CDS 40.0%
TAAGGGCTAACTCCATCTTA+TGG - Chr4:85232795-85232814 None:intergenic 40.0%
TAGAGTAACAGCAATCACAG+TGG + Chr4:85232399-85232418 MsG0480023446.01.T01:CDS 40.0%
TCGACTAAAACAACAAGCCT+TGG - Chr4:85232655-85232674 None:intergenic 40.0%
! AATGCCTCGTCTTTAGGAAA+AGG + Chr4:85232076-85232095 MsG0480023446.01.T01:CDS 40.0%
! CTGTGATTGCTGTTACTCTA+AGG - Chr4:85232399-85232418 None:intergenic 40.0%
! CTTTGTGGAGTTTTTCTGTG+AGG + Chr4:85232714-85232733 MsG0480023446.01.T01:CDS 40.0%
! GCTTGTTTGGTAAAGGGTAT+TGG + Chr4:85232980-85232999 MsG0480023446.01.T01:CDS 40.0%
!! AACAAGCCTTGGTGATGATT+TGG - Chr4:85232644-85232663 None:intergenic 40.0%
!! ACTGTAAGTTGGCTTTGTGT+TGG + Chr4:85233016-85233035 MsG0480023446.01.T01:CDS 40.0%
CAGTTCAACAAACAGGCAGA+AGG + Chr4:85232440-85232459 MsG0480023446.01.T01:CDS 45.0%
GAAGCCTCAGTTCAACAAAC+AGG + Chr4:85232433-85232452 MsG0480023446.01.T01:CDS 45.0%
TACATCAGTGGATGCCAAAC+AGG + Chr4:85232581-85232600 MsG0480023446.01.T01:CDS 45.0%
TCTGTGGAGTAGCCATAAGA+TGG + Chr4:85232780-85232799 MsG0480023446.01.T01:CDS 45.0%
! ATCGCCTTTTCCTAAAGACG+AGG - Chr4:85232083-85232102 None:intergenic 45.0%
! TCTGCCTGTTTGTTGAACTG+AGG - Chr4:85232440-85232459 None:intergenic 45.0%
! TTGAAGGGGTATAGACGTGT+TGG + Chr4:85232916-85232935 MsG0480023446.01.T01:CDS 45.0%
AATCCAATGGCCACCGTTGA+TGG + Chr4:85232342-85232361 MsG0480023446.01.T01:CDS 50.0%
GCAAAGTCAACCATCAACGG+TGG - Chr4:85232355-85232374 None:intergenic 50.0%
! GCGATTTTCGAAGCTACAGC+TGG + Chr4:85232277-85232296 MsG0480023446.01.T01:CDS 50.0%
CAACCATCAACGGTGGCCAT+TGG - Chr4:85232348-85232367 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 85231977 85233110 85231977 ID=MsG0480023446.01;Name=MsG0480023446.01
Chr4 mRNA 85231977 85233110 85231977 ID=MsG0480023446.01.T01;Parent=MsG0480023446.01;Name=MsG0480023446.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|377
Chr4 exon 85231977 85233110 85231977 ID=MsG0480023446.01.T01:exon:1436;Parent=MsG0480023446.01.T01
Chr4 CDS 85231977 85233110 85231977 ID=MsG0480023446.01.T01:cds;Parent=MsG0480023446.01.T01
Gene Sequence

>MsG0480023446.01.T01

ATGGAAGAGACAAGTTTAGTTGATCTTTTGCTGATAGGAGCTGAAGCTGCTGAATCACAAAACATGACTCTTGCTTCTGACATAATCGAAAAACTAAACAATGCCTCGTCTTTAGGAAAAGGCGATAGTTTATTGAACAGGTTGTGTCTTTTCTTTACGCAAGGATTGTATTATAAAACTACAAATGCTCCTAAGTTCCATTCAGAACATGTTTCTACACAGACAAGCACATTCTGTGTGTTTCAGATACTTCAAGAACTCTCTCCCTATGTAAAATTTGCTCATTTTACTGCCAATCAAGCGATTTTCGAAGCTACAGCTGGTGTCGAAGATGTTCATGTCATTGATTTTGATATCATGGAGGGAATCCAATGGCCACCGTTGATGGTTGACTTTGCAATGAGAAAGAAGACTACTTCCCTTAGAGTAACAGCAATCACAGTGGATCAGCAAAGTGAAGCCTCAGTTCAACAAACAGGCAGAAGGCTCAAAGAGTTCGCTGATTCAATCAATTTTCCGTTCACGTTCGATACAGTAATGATGGTGAGTGAAGAAGAATTCAAAGAAATTGAACTTGGTGAAACATTTATTGTCAATTGTATGATACATCAGTGGATGCCAAACAGGAGTTTCTCATTGATCAAAGCTTTTCTCGATCACGTAACCAAATCATCACCAAGGCTTGTTGTTTTAGTCGAAGAAGAACTGTTTAATTTTTCTAGACTCAAATCCATGTCCTTTGTGGAGTTTTTCTGTGAGGCTTTGCATCACTATATTGCAGTTTCTGATTCACTTGTAAGTACTCTGTGGAGTAGCCATAAGATGGAGTTAGCCCTTATAGAGAAAGAGGTATTGGGAAATAGAATCTTAGACAGTGTAAGACAGTTTCCTTGTGAAAAAGAGGAGAGAATATTGTGGGAAGAAAGATTTTTTTATTCATTGAAGGGGTATAGACGTGTTGGTATGAGTACATGCAATATTTCACAGGCTAAGCTATTGGTTAGCTTGTTTGGTAAAGGGTATTGGGTGCAATATGAGAACTGTAAGTTGGCTTTGTGTTGGAAGTCAAGACCTTTGACTTCTGTTTCAATCTGGGTACCAACAGATTATAATATGAGTAACAAAGTCAAATAG
Protein sequence

>MsG0480023446.01.T01

MEETSLVDLLLIGAEAAESQNMTLASDIIEKLNNASSLGKGDSLLNRLCLFFTQGLYYKTTNAPKFHSEHVSTQTSTFCVFQILQELSPYVKFAHFTANQAIFEATAGVEDVHVIDFDIMEGIQWPPLMVDFAMRKKTTSLRVTAITVDQQSEASVQQTGRRLKEFADSINFPFTFDTVMMVSEEEFKEIELGETFIVNCMIHQWMPNRSFSLIKAFLDHVTKSSPRLVVLVEEELFNFSRLKSMSFVEFFCEALHHYIAVSDSLVSTLWSSHKMELALIEKEVLGNRILDSVRQFPCEKEERILWEERFFYSLKGYRRVGMSTCNISQAKLLVSLFGKGYWVQYENCKLALCWKSRPLTSVSIWVPTDYNMSNKVK*