AlfalfaGEDB | MsG0480023456.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023456.01

KEGG: -
GO: GO:0046983
PFAM: PF00010

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023456.01.T01	XP_024627548.1	86.932	176	5	1	1	158	90	265	6.54E-105	311
MsG0480023456.01.T02	XP_024627548.1	94.34	265	13	1	1	263	1	265	7.81E-161	457
MsG0480023456.01.T03	XP_024627548.1	93.878	245	13	1	1	243	21	265	1.14E-144	415
MsG0480023456.01.T04	XP_024627548.1	96.698	212	5	1	1	210	54	265	5.73E-148	422
MsG0480023456.01.T05	PNX76884.1	86.726	113	15	0	1	113	120	232	1.30E-65	208

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023456.01.T01	O81313	81.818	88	16	0	1	88	88	175	5.81E-46	151
MsG0480023456.01.T02	Q9LXD8	81.651	109	13	1	85	186	92	200	2.19E-45	155
MsG0480023456.01.T03	Q9LXD8	81.651	109	13	1	65	166	92	200	1.22E-45	155
MsG0480023456.01.T04	Q9LXD8	81.651	109	13	1	32	133	92	200	4.21E-46	155

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023456.01.T01	A0A396GSG3	86.932	176	5	1	1	158	90	265	3.12e-105	311
MsG0480023456.01.T02	A0A396GSG3	94.340	265	13	1	1	263	1	265	3.73e-161	457
MsG0480023456.01.T03	A0A396GSG3	93.878	245	13	1	1	243	21	265	5.43e-145	415
MsG0480023456.01.T04	A0A396GSG3	96.698	212	5	1	1	210	54	265	2.74e-148	422
MsG0480023456.01.T05	A0A2K3LEF1	86.726	113	15	0	1	113	120	232	6.19e-66	208

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TFs/TRs:

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Gene ID	Type	Classification
MsG0480023456.01.T01	TF	bHLH
MsG0480023456.01.T04	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023456.01.T01	MTR_8g093500	86.628	172	5	1	1	154	90	261	1.26e-105	303
MsG0480023456.01.T01	MTR_3g101810	78.409	88	19	0	1	88	147	234	3.66e-46	152
MsG0480023456.01.T01	MTR_5g017210	67.647	102	33	0	1	102	113	214	7.29e-37	127
MsG0480023456.01.T01	MTR_8g015450	65.957	94	26	2	1	89	300	392	6.45e-29	110
MsG0480023456.01.T01	MTR_2g100490	64.444	90	31	1	1	89	265	354	6.95e-27	104
MsG0480023456.01.T01	MTR_7g069590	67.857	56	18	0	31	86	32	87	1.91e-21	85.1
MsG0480023456.01.T01	MTR_5g079500	53.165	79	35	2	11	87	202	280	3.41e-20	85.1
MsG0480023456.01.T01	MTR_1g018090	51.250	80	23	1	34	113	283	346	7.58e-20	84.7
MsG0480023456.01.T01	MTR_5g005110	66.071	56	19	0	34	89	233	288	1.20e-19	84.0
MsG0480023456.01.T01	MTR_1g106460	55.072	69	31	0	21	89	234	302	1.40e-19	83.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023456.01.T01	AT4G00120	81.818	88	16	0	1	88	88	175	5.93e-47	151
MsG0480023456.01.T01	AT5G09750	81.132	106	13	1	1	99	95	200	1.67e-45	149
MsG0480023456.01.T01	AT3G21330	67.416	89	29	0	1	89	245	333	6.48e-37	130
MsG0480023456.01.T01	AT5G67060	77.273	88	20	0	1	88	86	173	2.78e-36	125
MsG0480023456.01.T01	AT5G67060	77.273	88	20	0	1	88	98	185	5.37e-36	125
MsG0480023456.01.T01	AT3G50330	76.136	88	21	0	1	88	95	182	1.11e-35	124
MsG0480023456.01.T01	AT5G01305	64.179	67	24	0	23	89	35	101	6.52e-22	87.0
MsG0480023456.01.T01	AT5G43175	40.385	104	46	1	10	113	117	204	5.35e-20	83.2
MsG0480023456.01.T01	AT2G14760	52.500	80	22	1	34	113	246	309	6.03e-20	84.7
MsG0480023456.01.T01	AT4G33880	52.500	80	22	1	34	113	275	338	6.04e-20	85.1

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Find 54 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATTCATAGGAACTTGG+TGG	0.592411	4:-85421475	None:intergenic
ACCGGAATTCAATTTGACGC+CGG	0.643580	4:+85422070	MsG0480023456.01.T01:CDS
ACTCACTAATTACCTCATGA+TGG	0.592814	4:-85422138	None:intergenic
AGAAGAGTTAGGAGCTATGA+AGG	0.405429	4:+85421616	MsG0480023456.01.T02:CDS
AGTAGTAAATTTGGTGCAAA+AGG	0.442540	4:-85422013	None:intergenic
AGTGAACCTGATGAAGGTGG+TGG	0.679836	4:-85421545	None:intergenic
ATCAAGCTTTAACCATCATG+AGG	0.658321	4:+85422126	MsG0480023456.01.T01:CDS
ATCATGCTGTTGTAGCTATT+TGG	0.316175	4:-85421512	None:intergenic
ATCCTTCAAAGACTTGTTCC+TGG	0.399593	4:+85421788	MsG0480023456.01.T01:CDS
ATGAACATGTGAATGTCCTC+CGG	0.556908	4:-85422089	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAATTCCAGAAGAATTCAT+AGG	-	Chr4:85421488-85421507	None:intergenic	30.0%
CAAGAACTTAACATAACGAA+TGG	-	Chr4:85421852-85421871	None:intergenic	30.0%
CACTTGGAATCTTGAAAAAA+TGG	+	Chr4:85421412-85421431	MsG0480023456.01.T02:CDS	30.0%
GATCTTGTTGGAGAATAATA+CGG	-	Chr4:85421454-85421473	None:intergenic	30.0%
TTAATGCATTGTGATGAAAG+TGG	-	Chr4:85421938-85421957	None:intergenic	30.0%
AAGAATTCATAGGAACTTGG+TGG	-	Chr4:85421478-85421497	None:intergenic	35.0%
ACTCACTAATTACCTCATGA+TGG	-	Chr4:85422141-85422160	None:intergenic	35.0%
ATCATGCTGTTGTAGCTATT+TGG	-	Chr4:85421515-85421534	None:intergenic	35.0%
CAAGTTCCTATGAATTCTTC+TGG	+	Chr4:85421479-85421498	MsG0480023456.01.T02:CDS	35.0%
CAGAAGAATTCATAGGAACT+TGG	-	Chr4:85421481-85421500	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	85421371	85422162	85421371	ID=MsG0480023456.01;Name=MsG0480023456.01
Chr4	mRNA	85421632	85422162	85421632	ID=MsG0480023456.01.T01;Parent=MsG0480023456.01;Name=MsG0480023456.01.T01;_AED=0.20;_eAED=0.20;_QI=0\|0\|0\|1\|0\|0\|2\|0\|158
Chr4	exon	85421632	85421950	85421632	ID=MsG0480023456.01.T01:exon:5838;Parent=MsG0480023456.01.T01
Chr4	exon	85422005	85422162	85422005	ID=MsG0480023456.01.T01:exon:5839;Parent=MsG0480023456.01.T01
Chr4	CDS	85421632	85421950	85421632	ID=MsG0480023456.01.T01:cds;Parent=MsG0480023456.01.T01
Chr4	CDS	85422005	85422162	85422005	ID=MsG0480023456.01.T01:cds;Parent=MsG0480023456.01.T01
Chr4	mRNA	85421371	85422162	85421371	ID=MsG0480023456.01.T02;Parent=MsG0480023456.01;Name=MsG0480023456.01.T02;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|263
Chr4	exon	85421371	85422162	85421371	ID=MsG0480023456.01.T02:exon:5840;Parent=MsG0480023456.01.T02
Chr4	CDS	85421371	85422162	85421371	ID=MsG0480023456.01.T02:cds;Parent=MsG0480023456.01.T02
Chr4	mRNA	85421431	85422162	85421431	ID=MsG0480023456.01.T03;Parent=MsG0480023456.01;Name=MsG0480023456.01.T03;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|243
Chr4	exon	85421431	85422162	85421431	ID=MsG0480023456.01.T03:exon:5841;Parent=MsG0480023456.01.T03
Chr4	CDS	85421431	85422162	85421431	ID=MsG0480023456.01.T03:cds;Parent=MsG0480023456.01.T03
Chr4	mRNA	85421530	85422162	85421530	ID=MsG0480023456.01.T04;Parent=MsG0480023456.01;Name=MsG0480023456.01.T04;_AED=0.10;_eAED=0.10;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|210
Chr4	exon	85421530	85422162	85421530	ID=MsG0480023456.01.T04:exon:5842;Parent=MsG0480023456.01.T04
Chr4	CDS	85421530	85422162	85421530	ID=MsG0480023456.01.T04:cds;Parent=MsG0480023456.01.T04
Chr4	mRNA	85421821	85422162	85421821	ID=MsG0480023456.01.T05;Parent=MsG0480023456.01;Name=MsG0480023456.01.T05;_AED=0.29;_eAED=0.29;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|113
Chr4	exon	85421821	85422162	85421821	ID=MsG0480023456.01.T05:exon:5843;Parent=MsG0480023456.01.T05
Chr4	CDS	85421821	85422162	85421821	ID=MsG0480023456.01.T05:cds;Parent=MsG0480023456.01.T05

Gene Sequence

>MsG0480023456.01.T03

ATGGAAACAAATGAACAAAACCGTATTATTCTCCAACAAGATCACCACCAAGTTCCTATGAATTCTTCTGGAATTTGGCCACCAAATAGCTACAACAGCATGATCATGGCACCACCACCACCTTCATCAGGTTCACTCAGTGACATATTAGGGATCCACCGAATCGAAGATGTAGAAGAACAAGAAGAAGAGTTAGGAGCTATGAAGGAAATGATGTACAAGATTGCAGCTATGCAACCGGTGGATATCGACCCAGCAACGATTCGAAAACCAAAGCGAAGAAACGTTCGAATCAGCGACGATCCACAAAGTGTTGCAGCTAGACATAGAAGAGAAAGAATCAGTGAGAAGATTCGTATCCTTCAAAGACTTGTTCCTGGTGGAACTAAAATGGATACAGCTTCTATGCTTGATGAAGCCATTCGTTATGTTAAGTTCTTGAAGAGACAAATCAAGTTGCTTCAATCCACTCCTCAAAATCAACAACAACAACACATTCAATTGCCACTTTCATCACAATGCATTAATTCTACTCCTCCTAGTGCTTTGTTATTGTCTCCTTCTTCTGGTTGTGACGTCTGGCCTTTTGCACCAAATTTACTACTCACTTCCACAACAGCTGCCACAGTGGACTTACCCACCGGAATTCAATTTGACGCCGGAGGACATTCACATGTTCATGCTTGTGAAGGCTCATCAAGCTTTAACCATCATGAGGTAATTAGTGAGTGA

>MsG0480023456.01.T02

ATGGAAAATAACCAAAATAATACTAGTAGTGCATTCACAAGCACTTGGAATCTTGAAAAAATGGAAACAAATGAACAAAACCGTATTATTCTCCAACAAGATCACCACCAAGTTCCTATGAATTCTTCTGGAATTTGGCCACCAAATAGCTACAACAGCATGATCATGGCACCACCACCACCTTCATCAGGTTCACTCAGTGACATATTAGGGATCCACCGAATCGAAGATGTAGAAGAACAAGAAGAAGAGTTAGGAGCTATGAAGGAAATGATGTACAAGATTGCAGCTATGCAACCGGTGGATATCGACCCAGCAACGATTCGAAAACCAAAGCGAAGAAACGTTCGAATCAGCGACGATCCACAAAGTGTTGCAGCTAGACATAGAAGAGAAAGAATCAGTGAGAAGATTCGTATCCTTCAAAGACTTGTTCCTGGTGGAACTAAAATGGATACAGCTTCTATGCTTGATGAAGCCATTCGTTATGTTAAGTTCTTGAAGAGACAAATCAAGTTGCTTCAATCCACTCCTCAAAATCAACAACAACAACACATTCAATTGCCACTTTCATCACAATGCATTAATTCTACTCCTCCTAGTGCTTTGTTATTGTCTCCTTCTTCTGGTTGTGACGTCTGGCCTTTTGCACCAAATTTACTACTCACTTCCACAACAGCTGCCACAGTGGACTTACCCACCGGAATTCAATTTGACGCCGGAGGACATTCACATGTTCATGCTTGTGAAGGCTCATCAAGCTTTAACCATCATGAGGTAATTAGTGAGTGA

>MsG0480023456.01.T05

ATGGATACAGCTTCTATGCTTGATGAAGCCATTCGTTATGTTAAGTTCTTGAAGAGACAAATCAAGTTGCTTCAATCCACTCCTCAAAATCAACAACAACAACACATTCAATTGCCACTTTCATCACAATGCATTAATTCTACTCCTCCTAGTGCTTTGTTATTGTCTCCTTCTTCTGGTTGTGACGTCTGGCCTTTTGCACCAAATTTACTACTCACTTCCACAACAGCTGCCACAGTGGACTTACCCACCGGAATTCAATTTGACGCCGGAGGACATTCACATGTTCATGCTTGTGAAGGCTCATCAAGCTTTAACCATCATGAGGTAATTAGTGAGTGA

>MsG0480023456.01.T04

ATGATCATGGCACCACCACCACCTTCATCAGGTTCACTCAGTGACATATTAGGGATCCACCGAATCGAAGATGTAGAAGAACAAGAAGAAGAGTTAGGAGCTATGAAGGAAATGATGTACAAGATTGCAGCTATGCAACCGGTGGATATCGACCCAGCAACGATTCGAAAACCAAAGCGAAGAAACGTTCGAATCAGCGACGATCCACAAAGTGTTGCAGCTAGACATAGAAGAGAAAGAATCAGTGAGAAGATTCGTATCCTTCAAAGACTTGTTCCTGGTGGAACTAAAATGGATACAGCTTCTATGCTTGATGAAGCCATTCGTTATGTTAAGTTCTTGAAGAGACAAATCAAGTTGCTTCAATCCACTCCTCAAAATCAACAACAACAACACATTCAATTGCCACTTTCATCACAATGCATTAATTCTACTCCTCCTAGTGCTTTGTTATTGTCTCCTTCTTCTGGTTGTGACGTCTGGCCTTTTGCACCAAATTTACTACTCACTTCCACAACAGCTGCCACAGTGGACTTACCCACCGGAATTCAATTTGACGCCGGAGGACATTCACATGTTCATGCTTGTGAAGGCTCATCAAGCTTTAACCATCATGAGGTAATTAGTGAGTGA

>MsG0480023456.01.T01

ATGAAGGAAATGATGTACAAGATTGCAGCTATGCAACCGGTGGATATCGACCCAGCAACGATTCGAAAACCAAAGCGAAGAAACGTTCGAATCAGCGACGATCCACAAAGTGTTGCAGCTAGACATAGAAGAGAAAGAATCAGTGAGAAGATTCGTATCCTTCAAAGACTTGTTCCTGGTGGAACTAAAATGGATACAGCTTCTATGCTTGATGAAGCCATTCGTTATGTTAAGTTCTTGAAGAGACAAATCAAGTTGCTTCAATCCACTCCTCAAAATCAACAACAACAACACATTCAATTGCCACTTTCATCACAATACGTCTGGCCTTTTGCACCAAATTTACTACTCACTTCCACAACAGCTGCCACAGTGGACTTACCCACCGGAATTCAATTTGACGCCGGAGGACATTCACATGTTCATGCTTGTGAAGGCTCATCAAGCTTTAACCATCATGAGGTAATTAGTGAGTGA

Protein sequence

>MsG0480023456.01.T03

METNEQNRIILQQDHHQVPMNSSGIWPPNSYNSMIMAPPPPSSGSLSDILGIHRIEDVEEQEEELGAMKEMMYKIAAMQPVDIDPATIRKPKRRNVRISDDPQSVAARHRRERISEKIRILQRLVPGGTKMDTASMLDEAIRYVKFLKRQIKLLQSTPQNQQQQHIQLPLSSQCINSTPPSALLLSPSSGCDVWPFAPNLLLTSTTAATVDLPTGIQFDAGGHSHVHACEGSSSFNHHEVISE*

>MsG0480023456.01.T02

MENNQNNTSSAFTSTWNLEKMETNEQNRIILQQDHHQVPMNSSGIWPPNSYNSMIMAPPPPSSGSLSDILGIHRIEDVEEQEEELGAMKEMMYKIAAMQPVDIDPATIRKPKRRNVRISDDPQSVAARHRRERISEKIRILQRLVPGGTKMDTASMLDEAIRYVKFLKRQIKLLQSTPQNQQQQHIQLPLSSQCINSTPPSALLLSPSSGCDVWPFAPNLLLTSTTAATVDLPTGIQFDAGGHSHVHACEGSSSFNHHEVISE*

>MsG0480023456.01.T05

MDTASMLDEAIRYVKFLKRQIKLLQSTPQNQQQQHIQLPLSSQCINSTPPSALLLSPSSGCDVWPFAPNLLLTSTTAATVDLPTGIQFDAGGHSHVHACEGSSSFNHHEVISE*

>MsG0480023456.01.T04

MIMAPPPPSSGSLSDILGIHRIEDVEEQEEELGAMKEMMYKIAAMQPVDIDPATIRKPKRRNVRISDDPQSVAARHRRERISEKIRILQRLVPGGTKMDTASMLDEAIRYVKFLKRQIKLLQSTPQNQQQQHIQLPLSSQCINSTPPSALLLSPSSGCDVWPFAPNLLLTSTTAATVDLPTGIQFDAGGHSHVHACEGSSSFNHHEVISE*

>MsG0480023456.01.T01

MKEMMYKIAAMQPVDIDPATIRKPKRRNVRISDDPQSVAARHRRERISEKIRILQRLVPGGTKMDTASMLDEAIRYVKFLKRQIKLLQSTPQNQQQQHIQLPLSSQYVWPFAPNLLLTSTTAATVDLPTGIQFDAGGHSHVHACEGSSSFNHHEVISE*