AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023477.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023477.01.T01 MTR_8g093920 94.037 218 4 2 1 213 1 214 9.65e-151 417
MsG0480023477.01.T01 MTR_5g095740 77.477 111 25 0 19 129 7 117 1.30e-59 185
MsG0480023477.01.T01 MTR_3g058980 82.828 99 17 0 32 130 24 122 8.97e-59 183
MsG0480023477.01.T01 MTR_2g026710 78.704 108 21 1 34 141 16 121 1.06e-58 182
MsG0480023477.01.T01 MTR_8g091720 75.000 112 28 0 32 143 25 136 2.18e-58 183
MsG0480023477.01.T01 MTR_7g061270 78.947 95 20 0 34 128 25 119 3.27e-56 176
MsG0480023477.01.T01 MTR_7g100650 78.947 95 20 0 34 128 25 119 5.44e-56 176
MsG0480023477.01.T01 MTR_1g072790 65.487 113 39 0 16 128 10 122 7.71e-55 173
MsG0480023477.01.T01 MTR_4g133938 77.660 94 21 0 36 129 20 113 2.79e-53 167
MsG0480023477.01.T01 MTR_1g088860 75.000 96 24 0 34 129 35 130 1.21e-51 165
MsG0480023477.01.T01 MTR_0392s0020 64.545 110 39 0 27 136 10 119 1.36e-51 163
MsG0480023477.01.T01 MTR_4g119500 70.968 93 27 0 34 126 21 113 4.06e-48 154
MsG0480023477.01.T01 MTR_4g133952 61.616 99 38 0 34 132 57 155 3.49e-46 153
MsG0480023477.01.T01 MTR_1g039040 61.616 99 38 0 34 132 5 103 2.82e-44 146
MsG0480023477.01.T01 MTR_1g083070 60.606 99 39 0 33 131 2 100 3.62e-40 134
MsG0480023477.01.T01 MTR_1g029070 51.579 95 46 0 34 128 51 145 8.25e-29 106
MsG0480023477.01.T01 MTR_5g095900 42.222 90 52 0 34 123 15 104 2.82e-19 82.0
MsG0480023477.01.T01 MTR_1g028480 50.725 69 34 0 65 133 19 87 1.20e-18 78.2
MsG0480023477.01.T01 MTR_2g056000 29.197 137 82 2 26 147 23 159 1.12e-16 75.1
MsG0480023477.01.T01 MTR_1g029100 39.474 76 46 0 40 115 7 82 4.42e-16 70.9
MsG0480023477.01.T01 MTR_4g112380 28.409 88 63 0 36 123 12 99 4.42e-13 64.7
MsG0480023477.01.T02 MTR_8g093920 93.333 225 4 3 1 225 1 214 2.53e-153 424
MsG0480023477.01.T02 MTR_3g058980 76.364 110 26 0 33 142 13 122 1.76e-60 188
MsG0480023477.01.T02 MTR_5g095740 73.913 115 30 0 27 141 3 117 3.28e-60 187
MsG0480023477.01.T02 MTR_2g026710 78.704 108 21 1 46 153 16 121 4.37e-59 184
MsG0480023477.01.T02 MTR_8g091720 73.913 115 30 0 41 155 22 136 1.49e-58 184
MsG0480023477.01.T02 MTR_7g061270 71.560 109 30 1 33 140 11 119 6.01e-57 178
MsG0480023477.01.T02 MTR_7g100650 71.560 109 30 1 33 140 11 119 8.57e-57 178
MsG0480023477.01.T02 MTR_1g072790 72.000 100 28 0 41 140 23 122 4.06e-55 174
MsG0480023477.01.T02 MTR_4g133938 77.660 94 21 0 48 141 20 113 1.08e-53 169
MsG0480023477.01.T02 MTR_1g088860 67.857 112 35 1 31 141 19 130 5.33e-52 167
MsG0480023477.01.T02 MTR_0392s0020 66.990 103 34 0 46 148 17 119 1.93e-50 161
MsG0480023477.01.T02 MTR_4g119500 62.162 111 42 0 28 138 3 113 1.84e-49 158
MsG0480023477.01.T02 MTR_4g133952 58.036 112 47 0 33 144 44 155 3.01e-47 156
MsG0480023477.01.T02 MTR_1g039040 61.616 99 38 0 46 144 5 103 2.02e-44 147
MsG0480023477.01.T02 MTR_1g083070 60.606 99 39 0 45 143 2 100 4.31e-40 134
MsG0480023477.01.T02 MTR_1g029070 51.579 95 46 0 46 140 51 145 7.61e-29 107
MsG0480023477.01.T02 MTR_5g095900 41.053 95 56 0 41 135 10 104 1.71e-19 82.8
MsG0480023477.01.T02 MTR_1g028480 50.725 69 34 0 77 145 19 87 1.51e-18 78.2
MsG0480023477.01.T02 MTR_2g056000 28.571 140 91 2 26 159 23 159 3.18e-16 74.3
MsG0480023477.01.T02 MTR_1g029100 39.474 76 46 0 52 127 7 82 6.92e-16 70.9
MsG0480023477.01.T02 MTR_4g112380 32.051 78 53 0 48 125 12 89 5.36e-13 64.7
MsG0480023477.01.T03 MTR_8g093920 95.758 165 3 1 1 165 54 214 2.09e-115 326
MsG0480023477.01.T03 MTR_2g026710 77.419 93 19 1 1 93 31 121 2.72e-48 154
MsG0480023477.01.T03 MTR_3g058980 82.927 82 14 0 1 82 41 122 2.83e-48 155
MsG0480023477.01.T03 MTR_5g095740 84.146 82 13 0 1 82 37 118 3.21e-48 154
MsG0480023477.01.T03 MTR_8g091720 72.632 95 26 0 1 95 42 136 2.47e-47 153
MsG0480023477.01.T03 MTR_7g061270 78.750 80 17 0 1 80 40 119 5.99e-46 148
MsG0480023477.01.T03 MTR_7g100650 78.750 80 17 0 1 80 40 119 8.49e-46 148
MsG0480023477.01.T03 MTR_4g133938 76.543 81 19 0 1 81 33 113 4.14e-44 142
MsG0480023477.01.T03 MTR_1g072790 73.750 80 21 0 1 80 43 122 1.35e-43 142
MsG0480023477.01.T03 MTR_1g088860 71.605 81 23 0 1 81 50 130 9.82e-41 135
MsG0480023477.01.T03 MTR_0392s0020 62.500 88 33 0 1 88 32 119 9.79e-40 131
MsG0480023477.01.T03 MTR_4g119500 66.667 78 26 0 1 78 36 113 3.23e-37 125
MsG0480023477.01.T03 MTR_4g133952 58.333 84 35 0 1 84 72 155 1.08e-36 127
MsG0480023477.01.T03 MTR_1g039040 60.714 84 33 0 1 84 20 103 3.11e-36 124
MsG0480023477.01.T03 MTR_1g083070 57.831 83 35 0 1 83 18 100 1.41e-31 110
MsG0480023477.01.T03 MTR_1g029070 52.500 80 38 0 1 80 66 145 2.44e-24 93.2
MsG0480023477.01.T03 MTR_1g028480 50.725 69 34 0 17 85 19 87 4.33e-19 78.2
MsG0480023477.01.T03 MTR_5g095900 40.000 75 45 0 1 75 30 104 5.75e-14 66.6
MsG0480023477.01.T03 MTR_1g029100 38.806 67 41 0 1 67 16 82 4.61e-13 62.0
MsG0480023477.01.T03 MTR_2g056000 27.619 105 70 1 1 99 55 159 5.00e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023477.01.T01 AT2G13570 67.476 206 57 7 12 211 14 215 1.09e-72 219
MsG0480023477.01.T01 AT4G14540 79.091 110 23 0 27 136 13 122 1.74e-63 194
MsG0480023477.01.T01 AT5G47640 73.276 116 30 1 32 147 24 138 1.61e-59 185
MsG0480023477.01.T01 AT2G37060 78.947 95 20 0 34 128 29 123 5.98e-55 173
MsG0480023477.01.T01 AT2G37060 78.947 95 20 0 34 128 29 123 5.98e-55 173
MsG0480023477.01.T01 AT2G37060 78.947 95 20 0 34 128 29 123 5.98e-55 173
MsG0480023477.01.T01 AT3G53340 57.432 148 58 3 34 178 28 173 6.09e-55 173
MsG0480023477.01.T01 AT3G53340 57.432 148 58 3 34 178 28 173 6.09e-55 173
MsG0480023477.01.T01 AT2G38880 70.588 102 30 0 27 128 13 114 5.80e-54 169
MsG0480023477.01.T01 AT2G38880 64.348 115 41 0 27 141 13 127 7.05e-54 169
MsG0480023477.01.T01 AT2G38880 64.348 115 41 0 27 141 13 127 7.05e-54 169
MsG0480023477.01.T01 AT2G38880 64.348 115 41 0 27 141 13 127 7.05e-54 169
MsG0480023477.01.T01 AT3G53340 77.174 92 21 0 34 125 28 119 6.29e-53 166
MsG0480023477.01.T01 AT3G53340 77.419 93 21 0 34 126 28 120 1.94e-52 166
MsG0480023477.01.T01 AT3G53340 77.419 93 21 0 34 126 28 120 1.94e-52 166
MsG0480023477.01.T01 AT2G38880 70.707 99 29 0 27 125 13 111 3.58e-52 164
MsG0480023477.01.T01 AT2G38880 71.429 98 28 0 27 124 13 110 1.05e-51 164
MsG0480023477.01.T01 AT2G38880 70.707 99 29 0 27 125 13 111 1.06e-51 164
MsG0480023477.01.T01 AT2G38880 70.707 99 29 0 27 125 13 111 1.06e-51 164
MsG0480023477.01.T01 AT2G38880 70.707 99 29 0 27 125 13 111 2.36e-51 163
MsG0480023477.01.T01 AT2G38880 70.707 99 29 0 27 125 13 111 2.36e-51 163
MsG0480023477.01.T01 AT2G38880 70.707 99 29 0 27 125 13 111 2.36e-51 163
MsG0480023477.01.T01 AT2G47810 74.490 98 25 0 34 131 50 147 6.95e-51 162
MsG0480023477.01.T01 AT5G47670 63.542 96 35 0 34 129 28 123 1.97e-46 152
MsG0480023477.01.T01 AT5G47670 63.542 96 35 0 34 129 57 152 3.06e-46 153
MsG0480023477.01.T01 AT5G47670 63.542 96 35 0 34 129 57 152 3.06e-46 153
MsG0480023477.01.T01 AT1G09030 61.458 96 37 0 35 130 3 98 4.21e-42 139
MsG0480023477.01.T01 AT1G21970 60.417 96 38 0 34 129 58 153 1.91e-41 140
MsG0480023477.01.T01 AT5G23090 30.508 118 77 3 36 148 12 129 1.83e-14 68.6
MsG0480023477.01.T01 AT5G23090 30.508 118 77 3 36 148 12 129 1.83e-14 68.6
MsG0480023477.01.T01 AT5G23090 30.508 118 77 3 36 148 12 129 1.83e-14 68.6
MsG0480023477.01.T01 AT5G23090 30.682 88 61 0 36 123 12 99 2.01e-14 68.6
MsG0480023477.01.T01 AT5G08190 33.333 81 54 0 35 115 11 91 2.30e-14 68.6
MsG0480023477.01.T01 AT5G08190 33.333 81 54 0 35 115 11 91 2.78e-14 68.2
MsG0480023477.01.T02 AT2G13570 63.303 218 58 7 12 223 14 215 9.02e-71 215
MsG0480023477.01.T02 AT4G14540 72.358 123 33 1 26 148 1 122 5.93e-64 196
MsG0480023477.01.T02 AT5G47640 73.276 116 30 1 44 159 24 138 6.72e-60 187
MsG0480023477.01.T02 AT2G37060 78.947 95 20 0 46 140 29 123 2.59e-55 174
MsG0480023477.01.T02 AT2G37060 78.947 95 20 0 46 140 29 123 2.59e-55 174
MsG0480023477.01.T02 AT2G37060 78.947 95 20 0 46 140 29 123 2.59e-55 174
MsG0480023477.01.T02 AT3G53340 57.432 148 58 3 46 190 28 173 2.67e-55 174
MsG0480023477.01.T02 AT3G53340 57.432 148 58 3 46 190 28 173 2.67e-55 174
MsG0480023477.01.T02 AT2G38880 68.571 105 33 0 36 140 10 114 1.27e-54 172
MsG0480023477.01.T02 AT2G38880 68.571 105 33 0 36 140 10 114 2.08e-54 171
MsG0480023477.01.T02 AT2G38880 68.571 105 33 0 36 140 10 114 2.08e-54 171
MsG0480023477.01.T02 AT2G38880 68.571 105 33 0 36 140 10 114 2.08e-54 171
MsG0480023477.01.T02 AT3G53340 77.174 92 21 0 46 137 28 119 3.02e-53 167
MsG0480023477.01.T02 AT3G53340 77.419 93 21 0 46 138 28 120 9.30e-53 167
MsG0480023477.01.T02 AT3G53340 77.419 93 21 0 46 138 28 120 9.30e-53 167
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 37 137 11 111 1.24e-52 166
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 36 136 10 110 2.38e-52 166
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 37 137 11 111 4.29e-52 166
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 37 137 11 111 4.29e-52 166
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 37 137 11 111 1.29e-51 164
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 37 137 11 111 1.29e-51 164
MsG0480023477.01.T02 AT2G38880 69.307 101 31 0 37 137 11 111 1.29e-51 164
MsG0480023477.01.T02 AT2G47810 61.240 129 37 1 28 143 19 147 3.64e-51 163
MsG0480023477.01.T02 AT5G47670 57.143 112 48 0 30 141 12 123 2.88e-47 155
MsG0480023477.01.T02 AT5G47670 57.143 112 48 0 30 141 41 152 6.74e-47 155
MsG0480023477.01.T02 AT5G47670 57.143 112 48 0 30 141 41 152 6.74e-47 155
MsG0480023477.01.T02 AT1G09030 61.458 96 37 0 47 142 3 98 7.20e-42 139
MsG0480023477.01.T02 AT1G21970 60.417 96 38 0 46 141 58 153 1.46e-41 141
MsG0480023477.01.T02 AT5G23090 30.508 118 77 3 48 160 12 129 2.08e-14 68.9
MsG0480023477.01.T02 AT5G23090 30.508 118 77 3 48 160 12 129 2.08e-14 68.9
MsG0480023477.01.T02 AT5G23090 30.508 118 77 3 48 160 12 129 2.08e-14 68.9
MsG0480023477.01.T02 AT5G23090 34.615 78 51 0 48 125 12 89 2.61e-14 68.6
MsG0480023477.01.T02 AT5G08190 33.333 81 54 0 47 127 11 91 2.99e-14 68.6
MsG0480023477.01.T02 AT5G08190 33.333 81 54 0 47 127 11 91 3.11e-14 68.6
MsG0480023477.01.T03 AT2G13570 66.272 169 48 6 1 163 50 215 6.84e-61 187
MsG0480023477.01.T03 AT4G14540 81.818 88 16 0 1 88 35 122 3.35e-51 161
MsG0480023477.01.T03 AT5G47640 71.717 99 27 1 1 99 41 138 4.70e-49 157
MsG0480023477.01.T03 AT2G37060 78.750 80 17 0 1 80 44 123 7.99e-45 145
MsG0480023477.01.T03 AT2G37060 78.750 80 17 0 1 80 44 123 7.99e-45 145
MsG0480023477.01.T03 AT2G37060 78.750 80 17 0 1 80 44 123 7.99e-45 145
MsG0480023477.01.T03 AT3G53340 54.887 133 55 3 1 130 43 173 8.45e-45 145
MsG0480023477.01.T03 AT3G53340 54.887 133 55 3 1 130 43 173 8.45e-45 145
MsG0480023477.01.T03 AT2G38880 66.667 93 31 0 1 93 35 127 3.46e-44 143
MsG0480023477.01.T03 AT2G38880 66.667 93 31 0 1 93 35 127 5.00e-44 142
MsG0480023477.01.T03 AT2G38880 66.667 93 31 0 1 93 35 127 5.00e-44 142
MsG0480023477.01.T03 AT2G38880 66.667 93 31 0 1 93 35 127 5.00e-44 142
MsG0480023477.01.T03 AT3G53340 76.623 77 18 0 1 77 43 119 1.10e-42 138
MsG0480023477.01.T03 AT3G53340 76.923 78 18 0 1 78 43 120 2.39e-42 138
MsG0480023477.01.T03 AT3G53340 76.923 78 18 0 1 78 43 120 2.39e-42 138
MsG0480023477.01.T03 AT2G38880 75.325 77 19 0 1 77 35 111 6.15e-42 136
MsG0480023477.01.T03 AT2G38880 66.667 93 29 1 1 93 35 125 1.22e-41 136
MsG0480023477.01.T03 AT2G38880 75.325 77 19 0 1 77 35 111 1.36e-41 137
MsG0480023477.01.T03 AT2G38880 75.325 77 19 0 1 77 35 111 1.36e-41 137
MsG0480023477.01.T03 AT2G38880 75.325 77 19 0 1 77 35 111 3.18e-41 135
MsG0480023477.01.T03 AT2G38880 75.325 77 19 0 1 77 35 111 3.18e-41 135
MsG0480023477.01.T03 AT2G38880 75.325 77 19 0 1 77 35 111 3.18e-41 135
MsG0480023477.01.T03 AT2G47810 71.084 83 24 0 1 83 65 147 3.20e-40 133
MsG0480023477.01.T03 AT5G47670 60.494 81 32 0 1 81 43 123 5.88e-37 126
MsG0480023477.01.T03 AT5G47670 60.494 81 32 0 1 81 72 152 8.92e-37 127
MsG0480023477.01.T03 AT5G47670 60.494 81 32 0 1 81 72 152 8.92e-37 127
MsG0480023477.01.T03 AT1G21970 59.259 81 33 0 1 81 73 153 1.39e-33 119
MsG0480023477.01.T03 AT1G09030 59.756 82 33 0 1 82 17 98 3.58e-33 115
MsG0480023477.01.T03 AT5G23090 33.846 65 43 0 1 65 25 89 1.69e-11 59.7
MsG0480023477.01.T03 AT5G23090 33.846 65 43 0 1 65 25 89 1.69e-11 59.7
MsG0480023477.01.T03 AT5G23090 33.846 65 43 0 1 65 25 89 1.69e-11 59.7
MsG0480023477.01.T03 AT5G23090 33.846 65 43 0 1 65 25 89 1.72e-11 59.3
MsG0480023477.01.T03 AT5G08190 32.836 67 45 0 1 67 25 91 1.78e-11 59.7
MsG0480023477.01.T03 AT5G08190 32.836 67 45 0 1 67 25 91 1.90e-11 59.3

Find 23 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGGTGATGATATTATT+TGG 0.131471 4:+85728355 MsG0480023477.01.T02:CDS
TGTTCAGATCTTTGTTGTTT+TGG 0.178571 4:-85728471 None:intergenic
CAATGGTGATGATATTATTT+GGG 0.366036 4:+85728356 MsG0480023477.01.T02:CDS
ATTCTACCAACATTTGCTAT+AGG 0.386839 4:-85728195 None:intergenic
GGTTGATCAGTAGTCATATA+AGG 0.405322 4:-85728606 None:intergenic
GGAGAGAAAGGTAGTGGAAA+TGG 0.405478 4:-85728627 None:intergenic
AGTCATAGTAATTTGCCAAA+TGG 0.450337 4:+85728051 MsG0480023477.01.T02:CDS
ATGATGATTATGATTATTGT+TGG 0.460661 4:-85728133 None:intergenic
TGAATCGAATTTGGAGAGAA+AGG 0.466213 4:-85728639 None:intergenic
GTTAGGCTTTGAAGATTATG+TGG 0.475398 4:+85728389 MsG0480023477.01.T02:CDS
ACATTTATCCGATGCTTCCC+CGG 0.490805 4:-85728310 None:intergenic
ATTTGGGCTATTACAACGTT+AGG 0.507634 4:+85728372 MsG0480023477.01.T02:CDS
CAAGATCAGATTGATTCCAT+AGG 0.513351 4:+85728678 MsG0480023477.01.T02:CDS
GAATTTGGAGAGAAAGGTAG+TGG 0.535380 4:-85728633 None:intergenic
CTATGACTCTCATCATCCAT+TGG 0.535551 4:-85728036 None:intergenic
TCATTCATACCAATGCCCTA+TGG 0.542150 4:-85728694 None:intergenic
AGATTGATTCCATAGGGCAT+TGG 0.544180 4:+85728685 MsG0480023477.01.T02:CDS
TTTCTTCCTATAGCAAATGT+TGG 0.557368 4:+85728189 MsG0480023477.01.T02:CDS
AAGATCAGATTGATTCCATA+GGG 0.573421 4:+85728679 MsG0480023477.01.T02:CDS
GACAGTTCAAGAATGTGTGT+CGG 0.617021 4:+85728269 MsG0480023477.01.T02:CDS
ATGGAATCAATCTGATCTTG+CGG 0.627874 4:-85728675 None:intergenic
AACAACAAAGATCTGAACAA+AGG 0.635439 4:+85728475 MsG0480023477.01.T02:CDS
TGATCAGTAGTCATATAAGG+AGG 0.682713 4:-85728603 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATGATTATGATTATTGT+TGG - Chr4:85728136-85728155 None:intergenic 20.0%
!! CAAAAATATAGAGATATTGA+AGG + Chr4:85728432-85728451 MsG0480023477.01.T02:CDS 20.0%
!!! AATTGTTTTTGAATCGAATT+TGG - Chr4:85728651-85728670 None:intergenic 20.0%
!!! CAATATCTCTATATTTTTGA+AGG - Chr4:85728431-85728450 None:intergenic 20.0%
!!! TTTTGAAGGTAACATTTTAA+TGG - Chr4:85728417-85728436 None:intergenic 20.0%
!!! TTTTTAAACAATGACTTTCA+GGG - Chr4:85728092-85728111 None:intergenic 20.0%
! AAAAAAGTTATTCCTGCAAA+TGG + Chr4:85728222-85728241 MsG0480023477.01.T02:CDS 25.0%
! GAAAAAAGAAAGACAATCAA+TGG + Chr4:85728339-85728358 MsG0480023477.01.T02:CDS 25.0%
!! CAATGGTGATGATATTATTT+GGG + Chr4:85728356-85728375 MsG0480023477.01.T02:CDS 25.0%
!! GTTTTTAAACAATGACTTTC+AGG - Chr4:85728093-85728112 None:intergenic 25.0%
!! TCAATGGTGATGATATTATT+TGG + Chr4:85728355-85728374 MsG0480023477.01.T02:CDS 25.0%
AACAACAAAGATCTGAACAA+AGG + Chr4:85728475-85728494 MsG0480023477.01.T02:CDS 30.0%
TGCAAATGGAAAAATCTCAA+AGG + Chr4:85728236-85728255 MsG0480023477.01.T02:CDS 30.0%
TTTCTTCCTATAGCAAATGT+TGG + Chr4:85728189-85728208 MsG0480023477.01.T02:CDS 30.0%
! AAGATCAGATTGATTCCATA+GGG + Chr4:85728679-85728698 MsG0480023477.01.T02:CDS 30.0%
! AGTCATAGTAATTTGCCAAA+TGG + Chr4:85728051-85728070 MsG0480023477.01.T02:CDS 30.0%
! TTTTCTGTGTTGAATCCATT+TGG - Chr4:85728069-85728088 None:intergenic 30.0%
!! ATTCTACCAACATTTGCTAT+AGG - Chr4:85728198-85728217 None:intergenic 30.0%
!! TTTGAGATTTTTCCATTTGC+AGG - Chr4:85728237-85728256 None:intergenic 30.0%
!!! TGTTCAGATCTTTGTTGTTT+TGG - Chr4:85728474-85728493 None:intergenic 30.0%
ATGGAATCAATCTGATCTTG+CGG - Chr4:85728678-85728697 None:intergenic 35.0%
ATTTGGGCTATTACAACGTT+AGG + Chr4:85728372-85728391 MsG0480023477.01.T02:CDS 35.0%
GGTTGATCAGTAGTCATATA+AGG - Chr4:85728609-85728628 None:intergenic 35.0%
TGAATCGAATTTGGAGAGAA+AGG - Chr4:85728642-85728661 None:intergenic 35.0%
TGATCAGTAGTCATATAAGG+AGG - Chr4:85728606-85728625 None:intergenic 35.0%
! CAAGATCAGATTGATTCCAT+AGG + Chr4:85728678-85728697 MsG0480023477.01.T02:CDS 35.0%
!! AGTTCATAAGTTTTGTCACC+GGG + Chr4:85728292-85728311 MsG0480023477.01.T02:CDS 35.0%
!! GAGTTCATAAGTTTTGTCAC+CGG + Chr4:85728291-85728310 MsG0480023477.01.T02:CDS 35.0%
!! GTTAGGCTTTGAAGATTATG+TGG + Chr4:85728389-85728408 MsG0480023477.01.T02:CDS 35.0%
GAATTTGGAGAGAAAGGTAG+TGG - Chr4:85728636-85728655 None:intergenic 40.0%
GACAGTTCAAGAATGTGTGT+CGG + Chr4:85728269-85728288 MsG0480023477.01.T02:CDS 40.0%
TCATTCATACCAATGCCCTA+TGG - Chr4:85728697-85728716 None:intergenic 40.0%
! AGATTGATTCCATAGGGCAT+TGG + Chr4:85728685-85728704 MsG0480023477.01.T02:CDS 40.0%
!! GTTCATAAGTTTTGTCACCG+GGG + Chr4:85728293-85728312 MsG0480023477.01.T02:CDS 40.0%
ACATTTATCCGATGCTTCCC+CGG - Chr4:85728313-85728332 None:intergenic 45.0%
!! GGAGAGAAAGGTAGTGGAAA+TGG - Chr4:85728630-85728649 None:intergenic 45.0%
!! TTTTGTCACCGGGGAAGCAT+CGG + Chr4:85728302-85728321 MsG0480023477.01.T02:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 85728039 85728716 85728039 ID=MsG0480023477.01;Name=MsG0480023477.01
Chr4 mRNA 85728039 85728716 85728039 ID=MsG0480023477.01.T01;Parent=MsG0480023477.01;Name=MsG0480023477.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|0|0|2|0|213
Chr4 exon 85728039 85728127 85728039 ID=MsG0480023477.01.T01:exon:5944;Parent=MsG0480023477.01.T01
Chr4 exon 85728164 85728716 85728164 ID=MsG0480023477.01.T01:exon:5945;Parent=MsG0480023477.01.T01
Chr4 CDS 85728039 85728127 85728039 ID=MsG0480023477.01.T01:cds;Parent=MsG0480023477.01.T01
Chr4 CDS 85728164 85728716 85728164 ID=MsG0480023477.01.T01:cds;Parent=MsG0480023477.01.T01
Chr4 mRNA 85728039 85728716 85728039 ID=MsG0480023477.01.T02;Parent=MsG0480023477.01;Name=MsG0480023477.01.T02;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|0|1|0|225
Chr4 exon 85728039 85728716 85728039 ID=MsG0480023477.01.T02:exon:5946;Parent=MsG0480023477.01.T02
Chr4 CDS 85728039 85728716 85728039 ID=MsG0480023477.01.T02:cds;Parent=MsG0480023477.01.T02
Chr4 mRNA 85728219 85728716 85728219 ID=MsG0480023477.01.T03;Parent=MsG0480023477.01;Name=MsG0480023477.01.T03;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|0|1|0|165
Chr4 exon 85728219 85728716 85728219 ID=MsG0480023477.01.T03:exon:5947;Parent=MsG0480023477.01.T03
Chr4 CDS 85728219 85728716 85728219 ID=MsG0480023477.01.T03:cds;Parent=MsG0480023477.01.T03
Gene Sequence

>MsG0480023477.01.T01

ATGGATGATGAGAGTCATAGTAATTTGCCAAATGGATTCAACACAGAAAACCCTGAAAGTCATTGTTTAAAAACAATCAACAACAACAACAATAGCAATAAAGAACAAGATCGTTTTCTTCCTATAGCAAATGTTGGTAGAATAATGAAAAAAGTTATTCCTGCAAATGGAAAAATCTCAAAGGATGCAAAAGAGACAGTTCAAGAATGTGTGTCGGAGTTCATAAGTTTTGTCACCGGGGAAGCATCGGATAAATGTCAAAGAGAAAAAAGAAAGACAATCAATGGTGATGATATTATTTGGGCTATTACAACGTTAGGCTTTGAAGATTATGTGGAACCATTAAAATGTTACCTTCAAAAATATAGAGATATTGAAGGTGAAAAAGTCAATGTTCCAAAACAACAAAGATCTGAACAAAGGCTACATCAACATCAACATCAACATCAACATCAACATAATCAAGATGAATTAAATAATCAACATTTCAACAATAGTGTATATACATCTACAAATCTCATGTCTCAACCTCCTTATATGACTACTGATCAACCATTTCCACTACCTTTCTCTCCAAATTCGATTCAAAAACAATTACGACCGCAAGATCAGATTGATTCCATAGGGCATTGGTATGAATGA

>MsG0480023477.01.T02

ATGGATGATGAGAGTCATAGTAATTTGCCAAATGGATTCAACACAGAAAACCCTGAAAGTCATTGTTTAAAAACAATCAACAACAACAACAACACCAACAATAATCATAATCATCATCACAATAGCAATAGCAATAAAGAACAAGATCGTTTTCTTCCTATAGCAAATGTTGGTAGAATAATGAAAAAAGTTATTCCTGCAAATGGAAAAATCTCAAAGGATGCAAAAGAGACAGTTCAAGAATGTGTGTCGGAGTTCATAAGTTTTGTCACCGGGGAAGCATCGGATAAATGTCAAAGAGAAAAAAGAAAGACAATCAATGGTGATGATATTATTTGGGCTATTACAACGTTAGGCTTTGAAGATTATGTGGAACCATTAAAATGTTACCTTCAAAAATATAGAGATATTGAAGGTGAAAAAGTCAATGTTCCAAAACAACAAAGATCTGAACAAAGGCTACATCAACATCAACATCAACATCAACATCAACATAATCAAGATGAATTAAATAATCAACATTTCAACAATAGTGTATATACATCTACAAATCTCATGTCTCAACCTCCTTATATGACTACTGATCAACCATTTCCACTACCTTTCTCTCCAAATTCGATTCAAAAACAATTACGACCGCAAGATCAGATTGATTCCATAGGGCATTGGTATGAATGA

>MsG0480023477.01.T03

ATGAAAAAAGTTATTCCTGCAAATGGAAAAATCTCAAAGGATGCAAAAGAGACAGTTCAAGAATGTGTGTCGGAGTTCATAAGTTTTGTCACCGGGGAAGCATCGGATAAATGTCAAAGAGAAAAAAGAAAGACAATCAATGGTGATGATATTATTTGGGCTATTACAACGTTAGGCTTTGAAGATTATGTGGAACCATTAAAATGTTACCTTCAAAAATATAGAGATATTGAAGGTGAAAAAGTCAATGTTCCAAAACAACAAAGATCTGAACAAAGGCTACATCAACATCAACATCAACATCAACATCAACATAATCAAGATGAATTAAATAATCAACATTTCAACAATAGTGTATATACATCTACAAATCTCATGTCTCAACCTCCTTATATGACTACTGATCAACCATTTCCACTACCTTTCTCTCCAAATTCGATTCAAAAACAATTACGACCGCAAGATCAGATTGATTCCATAGGGCATTGGTATGAATGA
Protein sequence

>MsG0480023477.01.T01

MDDESHSNLPNGFNTENPESHCLKTINNNNNSNKEQDRFLPIANVGRIMKKVIPANGKISKDAKETVQECVSEFISFVTGEASDKCQREKRKTINGDDIIWAITTLGFEDYVEPLKCYLQKYRDIEGEKVNVPKQQRSEQRLHQHQHQHQHQHNQDELNNQHFNNSVYTSTNLMSQPPYMTTDQPFPLPFSPNSIQKQLRPQDQIDSIGHWYE*

>MsG0480023477.01.T02

MDDESHSNLPNGFNTENPESHCLKTINNNNNTNNNHNHHHNSNSNKEQDRFLPIANVGRIMKKVIPANGKISKDAKETVQECVSEFISFVTGEASDKCQREKRKTINGDDIIWAITTLGFEDYVEPLKCYLQKYRDIEGEKVNVPKQQRSEQRLHQHQHQHQHQHNQDELNNQHFNNSVYTSTNLMSQPPYMTTDQPFPLPFSPNSIQKQLRPQDQIDSIGHWYE*

>MsG0480023477.01.T03

MKKVIPANGKISKDAKETVQECVSEFISFVTGEASDKCQREKRKTINGDDIIWAITTLGFEDYVEPLKCYLQKYRDIEGEKVNVPKQQRSEQRLHQHQHQHQHQHNQDELNNQHFNNSVYTSTNLMSQPPYMTTDQPFPLPFSPNSIQKQLRPQDQIDSIGHWYE*