Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023574.01.T01 | XP_003630555.2 | 99.231 | 130 | 1 | 0 | 1 | 130 | 183 | 312 | 3.85E-94 | 284 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023574.01.T01 | Q8S8C6 | 76.154 | 130 | 31 | 0 | 1 | 130 | 167 | 296 | 5.64E-75 | 227 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023574.01.T01 | G7LI34 | 99.231 | 130 | 1 | 0 | 1 | 130 | 183 | 312 | 1.84e-94 | 284 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0480023574.01.T01 | TF | BBR-BPC |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023574.01.T01 | MTR_8g099780 | 99.231 | 130 | 1 | 0 | 1 | 130 | 183 | 312 | 4.67e-98 | 284 |
| MsG0480023574.01.T01 | MTR_1g023660 | 71.930 | 114 | 32 | 0 | 17 | 130 | 227 | 340 | 9.57e-64 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023574.01.T01 | AT2G21240 | 76.154 | 130 | 31 | 0 | 1 | 130 | 167 | 296 | 5.75e-76 | 227 |
| MsG0480023574.01.T01 | AT2G21240 | 76.154 | 130 | 31 | 0 | 1 | 130 | 167 | 296 | 5.75e-76 | 227 |
| MsG0480023574.01.T01 | AT4G38910 | 73.846 | 130 | 31 | 1 | 1 | 130 | 132 | 258 | 1.94e-71 | 214 |
| MsG0480023574.01.T01 | AT4G38910 | 73.846 | 130 | 31 | 1 | 1 | 130 | 157 | 283 | 4.57e-71 | 214 |
| MsG0480023574.01.T01 | AT5G42520 | 76.724 | 116 | 25 | 2 | 17 | 130 | 167 | 282 | 5.68e-64 | 196 |
| MsG0480023574.01.T01 | AT5G42520 | 76.724 | 116 | 25 | 2 | 17 | 130 | 223 | 338 | 7.32e-64 | 197 |
| MsG0480023574.01.T01 | AT5G42520 | 76.724 | 116 | 25 | 2 | 17 | 130 | 227 | 342 | 1.25e-63 | 197 |
| MsG0480023574.01.T01 | AT1G14685 | 52.137 | 117 | 56 | 0 | 14 | 130 | 163 | 279 | 6.41e-45 | 147 |
| MsG0480023574.01.T01 | AT1G14685 | 52.137 | 117 | 56 | 0 | 14 | 130 | 163 | 279 | 6.41e-45 | 147 |
| MsG0480023574.01.T01 | AT1G14685 | 52.137 | 117 | 56 | 0 | 14 | 130 | 163 | 279 | 6.41e-45 | 147 |
| MsG0480023574.01.T01 | AT1G14685 | 52.137 | 117 | 56 | 0 | 14 | 130 | 163 | 279 | 6.41e-45 | 147 |
| MsG0480023574.01.T01 | AT2G35550 | 54.310 | 116 | 53 | 0 | 15 | 130 | 111 | 226 | 1.18e-44 | 145 |
| MsG0480023574.01.T01 | AT2G35550 | 54.310 | 116 | 53 | 0 | 15 | 130 | 111 | 226 | 1.18e-44 | 145 |
| MsG0480023574.01.T01 | AT2G35550 | 54.310 | 116 | 53 | 0 | 15 | 130 | 111 | 226 | 1.18e-44 | 145 |
| MsG0480023574.01.T01 | AT2G35550 | 54.310 | 116 | 53 | 0 | 15 | 130 | 156 | 271 | 2.82e-44 | 145 |
| MsG0480023574.01.T01 | AT1G68120 | 55.769 | 104 | 46 | 0 | 27 | 130 | 166 | 269 | 3.18e-42 | 140 |
| MsG0480023574.01.T01 | AT2G01930 | 46.400 | 125 | 67 | 0 | 6 | 130 | 159 | 283 | 2.01e-41 | 139 |
| MsG0480023574.01.T01 | AT2G01930 | 46.400 | 125 | 67 | 0 | 6 | 130 | 159 | 283 | 2.01e-41 | 139 |
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTATACCATTGGATCTTA+AGG | 0.242442 | 4:+87120996 | MsG0480023574.01.T01:CDS |
| CATTGGATCTTAAGGATTAC+TGG | 0.283657 | 4:+87121004 | MsG0480023574.01.T01:CDS |
| ACTCCAGTACACGTGCAAAC+TGG | 0.357093 | 4:-87120790 | None:intergenic |
| AAATGGGCGAGGATTTAAAT+AGG | 0.362648 | 4:+87120671 | None:intergenic |
| GTAACGATTTGTTCCATGTC+TGG | 0.409057 | 4:-87121028 | None:intergenic |
| GTCCCAGTTTGCACGTGTAC+TGG | 0.410401 | 4:+87120787 | MsG0480023574.01.T01:CDS |
| ATGCGAGCATGGCGCTTGTT+CGG | 0.428637 | 4:-87120901 | None:intergenic |
| ACCACGACAGTGCTACAAAT+GGG | 0.437083 | 4:+87120813 | MsG0480023574.01.T01:CDS |
| AAGCGCCATGCTCGCATTGG+AGG | 0.439433 | 4:+87120907 | MsG0480023574.01.T01:CDS |
| CTCCAGTACACGTGCAAACT+GGG | 0.457013 | 4:-87120789 | None:intergenic |
| GACAAAGATCAAATCTGAAT+GGG | 0.471460 | 4:+87120711 | MsG0480023574.01.T01:CDS |
| CAAATCTGAATGGGATAGGC+AGG | 0.475987 | 4:+87120720 | MsG0480023574.01.T01:CDS |
| CCATGATTTATCTATACCAT+TGG | 0.476445 | 4:+87120987 | MsG0480023574.01.T01:CDS |
| GGACAAAGATCAAATCTGAA+TGG | 0.478623 | 4:+87120710 | MsG0480023574.01.T01:CDS |
| AACAAGCGCCATGCTCGCAT+TGG | 0.488361 | 4:+87120904 | MsG0480023574.01.T01:CDS |
| TCCGCCCTCCAATGCGAGCA+TGG | 0.489587 | 4:-87120912 | None:intergenic |
| TACCACGACAGTGCTACAAA+TGG | 0.496139 | 4:+87120812 | MsG0480023574.01.T01:CDS |
| AATAGGAAGGCTTATTCTGA+AGG | 0.504268 | 4:+87120688 | MsG0480023574.01.T01:CDS |
| CTCAGTAGGTTTGCATCAGA+AGG | 0.511971 | 4:+87120964 | MsG0480023574.01.T01:CDS |
| AGCGCCATGCTCGCATTGGA+GGG | 0.517752 | 4:+87120908 | MsG0480023574.01.T01:CDS |
| AGATCAAATCTGAATGGGAT+AGG | 0.533258 | 4:+87120716 | MsG0480023574.01.T01:CDS |
| TTGTTCGGAAGCTGTGGTAG+TGG | 0.541295 | 4:-87120886 | None:intergenic |
| CAAAATAAAGAAAATGGGCG+AGG | 0.545395 | 4:+87120660 | None:intergenic |
| CCAATGGTATAGATAAATCA+TGG | 0.547636 | 4:-87120987 | None:intergenic |
| CCCCATTTGTAGCACTGTCG+TGG | 0.555932 | 4:-87120814 | None:intergenic |
| GGGCGAGGATTTAAATAGGA+AGG | 0.570874 | 4:+87120675 | MsG0480023574.01.T01:CDS |
| AAGGATTACTGGGCCAGACA+TGG | 0.576589 | 4:+87121015 | MsG0480023574.01.T01:CDS |
| GCCATGCTCGCATTGGAGGG+CGG | 0.577730 | 4:+87120911 | MsG0480023574.01.T01:CDS |
| GTACACGTGCAAACTGGGAC+AGG | 0.585669 | 4:-87120784 | None:intergenic |
| ATAGGAAGGCTTATTCTGAA+GGG | 0.587900 | 4:+87120689 | MsG0480023574.01.T01:CDS |
| ATTGGATCTTAAGGATTACT+GGG | 0.588784 | 4:+87121005 | MsG0480023574.01.T01:CDS |
| GAATGGGATAGGCAGGATGT+TGG | 0.593838 | 4:+87120727 | MsG0480023574.01.T01:CDS |
| CAGTAATCCTTAAGATCCAA+TGG | 0.595456 | 4:-87121003 | None:intergenic |
| TGGCGCTTGTTCGGAAGCTG+TGG | 0.609294 | 4:-87120892 | None:intergenic |
| CCACGACAGTGCTACAAATG+GGG | 0.618535 | 4:+87120814 | MsG0480023574.01.T01:CDS |
| ATTGGAGGGCGGAAAATGAG+CGG | 0.633747 | 4:+87120922 | MsG0480023574.01.T01:CDS |
| CACGACAGTGCTACAAATGG+GGG | 0.651085 | 4:+87120815 | MsG0480023574.01.T01:CDS |
| TGGATGCATTGACAATGTAG+TGG | 0.702687 | 4:-87120866 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCTATACCATTGGATCTTA+AGG | + | Chr4:87120996-87121015 | MsG0480023574.01.T01:CDS | 30.0% | |
| ATTGGATCTTAAGGATTACT+GGG | + | Chr4:87121005-87121024 | MsG0480023574.01.T01:CDS | 30.0% | |
| CCAATGGTATAGATAAATCA+TGG | - | Chr4:87120990-87121009 | None:intergenic | 30.0% | |
| CCATGATTTATCTATACCAT+TGG | + | Chr4:87120987-87121006 | MsG0480023574.01.T01:CDS | 30.0% | |
| GACAAAGATCAAATCTGAAT+GGG | + | Chr4:87120711-87120730 | MsG0480023574.01.T01:CDS | 30.0% | |
| AATAGGAAGGCTTATTCTGA+AGG | + | Chr4:87120688-87120707 | MsG0480023574.01.T01:CDS | 35.0% | |
| AGATCAAATCTGAATGGGAT+AGG | + | Chr4:87120716-87120735 | MsG0480023574.01.T01:CDS | 35.0% | |
| ATAGGAAGGCTTATTCTGAA+GGG | + | Chr4:87120689-87120708 | MsG0480023574.01.T01:CDS | 35.0% | |
| CAGTAATCCTTAAGATCCAA+TGG | - | Chr4:87121006-87121025 | None:intergenic | 35.0% | |
| CATTGGATCTTAAGGATTAC+TGG | + | Chr4:87121004-87121023 | MsG0480023574.01.T01:CDS | 35.0% | |
| GGACAAAGATCAAATCTGAA+TGG | + | Chr4:87120710-87120729 | MsG0480023574.01.T01:CDS | 35.0% | |
| ! | TTTTTAGACGACTTCTCAGT+AGG | + | Chr4:87120950-87120969 | MsG0480023574.01.T01:CDS | 35.0% |
| ! | GTAACGATTTGTTCCATGTC+TGG | - | Chr4:87121031-87121050 | None:intergenic | 40.0% |
| ! | TGGATGCATTGACAATGTAG+TGG | - | Chr4:87120869-87120888 | None:intergenic | 40.0% |
| ACCACGACAGTGCTACAAAT+GGG | + | Chr4:87120813-87120832 | MsG0480023574.01.T01:CDS | 45.0% | |
| CAAATCTGAATGGGATAGGC+AGG | + | Chr4:87120720-87120739 | MsG0480023574.01.T01:CDS | 45.0% | |
| CTCAGTAGGTTTGCATCAGA+AGG | + | Chr4:87120964-87120983 | MsG0480023574.01.T01:CDS | 45.0% | |
| GGGCGAGGATTTAAATAGGA+AGG | + | Chr4:87120675-87120694 | MsG0480023574.01.T01:CDS | 45.0% | |
| TACCACGACAGTGCTACAAA+TGG | + | Chr4:87120812-87120831 | MsG0480023574.01.T01:CDS | 45.0% | |
| AAGGATTACTGGGCCAGACA+TGG | + | Chr4:87121015-87121034 | MsG0480023574.01.T01:CDS | 50.0% | |
| ACTCCAGTACACGTGCAAAC+TGG | - | Chr4:87120793-87120812 | None:intergenic | 50.0% | |
| ATTGGAGGGCGGAAAATGAG+CGG | + | Chr4:87120922-87120941 | MsG0480023574.01.T01:CDS | 50.0% | |
| CACGACAGTGCTACAAATGG+GGG | + | Chr4:87120815-87120834 | MsG0480023574.01.T01:CDS | 50.0% | |
| CAGTGCTACAAATGGGGGAA+TGG | + | Chr4:87120820-87120839 | MsG0480023574.01.T01:CDS | 50.0% | |
| CCACGACAGTGCTACAAATG+GGG | + | Chr4:87120814-87120833 | MsG0480023574.01.T01:CDS | 50.0% | |
| CTCCAGTACACGTGCAAACT+GGG | - | Chr4:87120792-87120811 | None:intergenic | 50.0% | |
| GAATGGGATAGGCAGGATGT+TGG | + | Chr4:87120727-87120746 | MsG0480023574.01.T01:CDS | 50.0% | |
| TGCTACAAATGGGGGAATGG+TGG | + | Chr4:87120823-87120842 | MsG0480023574.01.T01:CDS | 50.0% | |
| TTGTTCGGAAGCTGTGGTAG+TGG | - | Chr4:87120889-87120908 | None:intergenic | 50.0% | |
| ! | ACAAATGGGGGAATGGTGGA+TGG | + | Chr4:87120827-87120846 | MsG0480023574.01.T01:CDS | 50.0% |
| AACAAGCGCCATGCTCGCAT+TGG | + | Chr4:87120904-87120923 | MsG0480023574.01.T01:CDS | 55.0% | |
| ATGCGAGCATGGCGCTTGTT+CGG | - | Chr4:87120904-87120923 | None:intergenic | 55.0% | |
| CCCCATTTGTAGCACTGTCG+TGG | - | Chr4:87120817-87120836 | None:intergenic | 55.0% | |
| GTACACGTGCAAACTGGGAC+AGG | - | Chr4:87120787-87120806 | None:intergenic | 55.0% | |
| GTCCCAGTTTGCACGTGTAC+TGG | + | Chr4:87120787-87120806 | MsG0480023574.01.T01:CDS | 55.0% | |
| AAGCGCCATGCTCGCATTGG+AGG | + | Chr4:87120907-87120926 | MsG0480023574.01.T01:CDS | 60.0% | |
| AGCGCCATGCTCGCATTGGA+GGG | + | Chr4:87120908-87120927 | MsG0480023574.01.T01:CDS | 60.0% | |
| TGGCGCTTGTTCGGAAGCTG+TGG | - | Chr4:87120895-87120914 | None:intergenic | 60.0% | |
| GCCATGCTCGCATTGGAGGG+CGG | + | Chr4:87120911-87120930 | MsG0480023574.01.T01:CDS | 65.0% | |
| TCCGCCCTCCAATGCGAGCA+TGG | - | Chr4:87120915-87120934 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 87120673 | 87121065 | 87120673 | ID=MsG0480023574.01;Name=MsG0480023574.01 |
| Chr4 | mRNA | 87120673 | 87121065 | 87120673 | ID=MsG0480023574.01.T01;Parent=MsG0480023574.01;Name=MsG0480023574.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|130 |
| Chr4 | exon | 87120673 | 87121065 | 87120673 | ID=MsG0480023574.01.T01:exon:2997;Parent=MsG0480023574.01.T01 |
| Chr4 | CDS | 87120673 | 87121065 | 87120673 | ID=MsG0480023574.01.T01:cds;Parent=MsG0480023574.01.T01 |
| Gene Sequence |
| Protein sequence |
