Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023852.01.T01 | MCI33018.1 | 80.488 | 41 | 8 | 0 | 1 | 41 | 1 | 41 | 1.61E-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023852.01.T01 | A0A392R8Q9 | 80.488 | 41 | 8 | 0 | 1 | 41 | 1 | 41 | 7.67e-14 | 71.2 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0480023852.01.T01 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023852.01.T01 | MTR_8g103520 | 82.927 | 41 | 7 | 0 | 1 | 41 | 213 | 253 | 7.40e-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGTTTACACAGAGATT+TGG | 0.279666 | 4:+90481143 | MsG0480023852.01.T01:CDS |
| CACCACCGCGAAAGTGTCTT+TGG | 0.330689 | 4:-90480774 | None:intergenic |
| TGAAGTTTACACAGAGATTT+GGG | 0.390881 | 4:+90481144 | MsG0480023852.01.T01:CDS |
| GGGAAGCTTTATTCTCTGAC+TGG | 0.460429 | 4:+90481164 | MsG0480023852.01.T01:CDS |
| ATGGGTGTTCTTCCGTTGAA+CGG | 0.516099 | 4:+90480745 | MsG0480023852.01.T01:CDS |
| GAAGTTCTAAAATCATTGTA+CGG | 0.539349 | 4:-90480811 | None:intergenic |
| AAAAGATTCTCCAACTCACT+TGG | 0.551265 | 4:-90480873 | None:intergenic |
| AAAGTCCAAAGACACTTTCG+CGG | 0.641247 | 4:+90480769 | MsG0480023852.01.T01:CDS |
| GTCCAAAGACACTTTCGCGG+TGG | 0.682705 | 4:+90480772 | MsG0480023852.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAACAACAAAAAAAAAAT+AGG | + | Chr4:90481191-90481210 | MsG0480023852.01.T01:CDS | 15.0% |
| !! | ACAACAACAAAAAAAAAATA+GGG | + | Chr4:90481192-90481211 | MsG0480023852.01.T01:CDS | 15.0% |
| !! | TAACAACAACAAAAAAAATA+GGG | + | Chr4:90480988-90481007 | MsG0480023852.01.T01:intron | 15.0% |
| !! | TTAACAACAACAAAAAAAAT+AGG | + | Chr4:90480987-90481006 | MsG0480023852.01.T01:intron | 15.0% |
| !!! | TCTGTTATTATTATTAGTTT+TGG | + | Chr4:90481053-90481072 | MsG0480023852.01.T01:intron | 15.0% |
| !!! | CAATGATTTTAGAACTTCAA+TGG | + | Chr4:90480816-90480835 | MsG0480023852.01.T01:CDS | 25.0% |
| !!! | GAAGTTCTAAAATCATTGTA+CGG | - | Chr4:90480814-90480833 | None:intergenic | 25.0% |
| TGAAGTTTACACAGAGATTT+GGG | + | Chr4:90480943-90480962 | MsG0480023852.01.T01:intron | 30.0% | |
| TTGAAGTTTACACAGAGATT+TGG | + | Chr4:90480942-90480961 | MsG0480023852.01.T01:intron | 30.0% | |
| !!! | TATTTTTTCACCAAGTGAGT+TGG | + | Chr4:90480863-90480882 | MsG0480023852.01.T01:CDS | 30.0% |
| !!! | TGATTTTAGAACTTCAATGG+TGG | + | Chr4:90480819-90480838 | MsG0480023852.01.T01:CDS | 30.0% |
| AAAAGATTCTCCAACTCACT+TGG | - | Chr4:90480876-90480895 | None:intergenic | 35.0% | |
| AAAGTCCAAAGACACTTTCG+CGG | + | Chr4:90480769-90480788 | MsG0480023852.01.T01:CDS | 40.0% | |
| ! | CTTTGGACTTTTCCGTTCAA+CGG | - | Chr4:90480760-90480779 | None:intergenic | 40.0% |
| ATGGGTGTTCTTCCGTTGAA+CGG | + | Chr4:90480745-90480764 | MsG0480023852.01.T01:CDS | 45.0% | |
| !! | GGGAAGCTTTATTCTCTGAC+TGG | + | Chr4:90480963-90480982 | MsG0480023852.01.T01:intron | 45.0% |
| GTCCAAAGACACTTTCGCGG+TGG | + | Chr4:90480772-90480791 | MsG0480023852.01.T01:CDS | 55.0% | |
| !! | CACCACCGCGAAAGTGTCTT+TGG | - | Chr4:90480777-90480796 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 90480745 | 90481295 | 90480745 | ID=MsG0480023852.01;Name=MsG0480023852.01 |
| Chr4 | mRNA | 90480745 | 90481295 | 90480745 | ID=MsG0480023852.01.T01;Parent=MsG0480023852.01;Name=MsG0480023852.01.T01;_AED=0.36;_eAED=0.73;_QI=0|0|0|1|0|0|2|0|116 |
| Chr4 | exon | 90480745 | 90480899 | 90480745 | ID=MsG0480023852.01.T01:exon:15233;Parent=MsG0480023852.01.T01 |
| Chr4 | exon | 90481100 | 90481295 | 90481100 | ID=MsG0480023852.01.T01:exon:15234;Parent=MsG0480023852.01.T01 |
| Chr4 | CDS | 90480745 | 90480899 | 90480745 | ID=MsG0480023852.01.T01:cds;Parent=MsG0480023852.01.T01 |
| Chr4 | CDS | 90481100 | 90481295 | 90481100 | ID=MsG0480023852.01.T01:cds;Parent=MsG0480023852.01.T01 |
| Gene Sequence |
| Protein sequence |
