AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024071.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024071.01.T01 MTR_6g051425 74.900 251 59 1 1 247 1 251 2.27e-142 402
MsG0580024071.01.T01 MTR_2g039370 83.784 74 12 0 49 122 98 171 4.52e-38 135
MsG0580024071.01.T01 MTR_3g465760 72.464 69 15 1 41 105 2 70 6.40e-28 102
MsG0580024071.01.T01 MTR_0558s0020 28.033 239 153 4 28 247 47 285 8.35e-28 108
MsG0580024071.01.T01 MTR_1g070355 29.204 226 150 6 27 247 84 304 1.38e-25 105
MsG0580024071.01.T01 MTR_8g446020 28.922 204 133 2 56 247 42 245 4.79e-25 99.8
MsG0580024071.01.T01 MTR_7g446520 30.120 249 148 4 2 247 77 302 5.00e-25 101
MsG0580024071.01.T01 MTR_4g035820 30.290 241 150 7 18 247 76 309 1.84e-24 102
MsG0580024071.01.T01 MTR_7g446480 34.932 146 95 0 101 246 149 294 3.72e-23 95.9
MsG0580024071.01.T01 MTR_7g446510 32.778 180 120 1 67 246 131 309 7.68e-23 95.5
MsG0580024071.01.T01 MTR_6g090505 29.000 200 126 3 64 247 21 220 3.86e-22 94.0
MsG0580024071.01.T01 MTR_1g013060 27.536 207 140 4 19 218 26 229 1.00e-21 90.5
MsG0580024071.01.T01 MTR_5g076070 27.232 224 151 5 29 246 65 282 4.63e-21 89.7
MsG0580024071.01.T01 MTR_1g083640 26.201 229 146 6 22 229 56 282 1.34e-20 88.6
MsG0580024071.01.T01 MTR_3g450850 25.620 242 166 6 17 246 47 286 4.96e-17 79.7
MsG0580024071.01.T01 MTR_0402s0040 30.409 171 103 6 29 191 38 200 4.13e-16 77.4
MsG0580024071.01.T01 MTR_0337s0040 28.472 144 102 1 83 225 81 224 1.23e-15 73.9
MsG0580024071.01.T01 MTR_5g039760 26.263 198 112 5 19 211 12 180 2.67e-13 67.0
MsG0580024071.01.T01 MTR_2g098880 31.405 121 73 3 7 121 51 167 3.68e-13 67.4
MsG0580024071.01.T01 MTR_2g098880 31.405 121 73 3 7 121 63 179 3.88e-13 67.8
MsG0580024071.01.T01 MTR_2g098920 30.097 103 71 1 18 120 81 182 1.97e-12 65.5
MsG0580024071.01.T01 MTR_1g008290 26.316 114 83 1 101 214 32 144 4.89e-12 62.4
MsG0580024071.01.T01 MTR_2g014100 24.873 197 129 4 15 192 42 238 7.28e-12 63.9
MsG0580024071.01.T01 MTR_4g067290 27.928 111 79 1 14 124 53 162 6.10e-11 60.8
MsG0580024071.01.T01 MTR_4g067290 27.928 111 79 1 14 124 73 182 6.55e-11 61.2
MsG0580024071.01.T01 MTR_4g067290 27.928 111 79 1 14 124 53 162 6.87e-11 60.8
MsG0580024071.01.T01 MTR_4g067290 27.928 111 79 1 14 124 73 182 7.02e-11 61.2
MsG0580024071.01.T01 MTR_4g067290 27.928 111 79 1 14 124 71 180 7.10e-11 61.2
MsG0580024071.01.T01 MTR_4g067290 27.928 111 79 1 14 124 113 222 7.24e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024071.01.T01 AT4G38180 36.207 232 142 4 20 247 76 305 2.34e-38 142
MsG0580024071.01.T01 AT4G15090 30.901 233 143 5 24 247 58 281 1.94e-26 107
MsG0580024071.01.T01 AT4G15090 30.901 233 143 5 24 247 58 281 1.94e-26 107
MsG0580024071.01.T01 AT4G15090 30.901 233 143 5 24 247 58 281 1.94e-26 107
MsG0580024071.01.T01 AT4G15090 30.901 233 143 5 24 247 58 281 1.94e-26 107
MsG0580024071.01.T01 AT4G15090 30.901 233 143 5 24 247 58 281 1.94e-26 107
MsG0580024071.01.T01 AT1G10240 30.085 236 158 4 18 247 49 283 3.57e-23 98.6
MsG0580024071.01.T01 AT1G10240 30.085 236 158 4 18 247 49 283 3.57e-23 98.6
MsG0580024071.01.T01 AT1G76320 30.942 223 141 5 27 247 7 218 1.17e-22 97.1
MsG0580024071.01.T01 AT1G76320 30.942 223 141 5 27 247 7 218 1.19e-22 97.1
MsG0580024071.01.T01 AT1G76320 30.942 223 141 5 27 247 7 218 1.20e-22 97.1
MsG0580024071.01.T01 AT1G76320 30.942 223 141 5 27 247 7 218 1.20e-22 97.1
MsG0580024071.01.T01 AT5G28530 28.571 245 158 4 18 247 56 298 2.69e-21 92.8
MsG0580024071.01.T01 AT5G28530 28.571 245 158 4 18 247 56 298 2.69e-21 92.8
MsG0580024071.01.T01 AT5G28530 28.571 245 158 4 18 247 56 298 2.72e-21 92.8
MsG0580024071.01.T01 AT2G27110 28.205 234 141 5 13 246 45 251 6.20e-21 92.0
MsG0580024071.01.T01 AT2G27110 28.205 234 141 5 13 246 45 251 6.20e-21 92.0
MsG0580024071.01.T01 AT3G06250 28.017 232 128 4 16 247 189 381 1.32e-20 90.9
MsG0580024071.01.T01 AT3G06250 28.017 232 128 4 16 247 189 381 1.32e-20 90.9
MsG0580024071.01.T01 AT3G06250 28.017 232 128 4 16 247 189 381 1.32e-20 90.9
MsG0580024071.01.T01 AT4G19990 26.394 269 135 9 1 247 1 228 3.81e-20 89.7
MsG0580024071.01.T01 AT4G19990 26.394 269 135 9 1 247 1 228 3.81e-20 89.7
MsG0580024071.01.T01 AT4G19990 26.394 269 135 9 1 247 1 228 4.28e-20 89.4
MsG0580024071.01.T01 AT4G19990 26.394 269 135 9 1 247 1 228 4.28e-20 89.4
MsG0580024071.01.T01 AT4G19990 26.394 269 135 9 1 247 1 228 4.61e-20 89.4
MsG0580024071.01.T01 AT1G52520 30.000 230 147 6 19 247 87 303 6.79e-20 89.0
MsG0580024071.01.T01 AT4G19990 26.415 265 132 9 5 247 19 242 9.72e-20 88.6
MsG0580024071.01.T01 AT4G19990 30.488 164 78 4 84 247 44 171 5.30e-17 80.5
MsG0580024071.01.T01 AT1G80010 27.426 237 156 6 20 247 98 327 6.07e-17 80.1
MsG0580024071.01.T01 AT3G22170 27.542 236 129 7 28 247 81 290 6.93e-16 77.0
MsG0580024071.01.T01 AT3G22170 27.542 236 129 7 28 247 81 290 6.93e-16 77.0
MsG0580024071.01.T01 AT5G28530 29.282 181 119 4 18 192 56 233 6.57e-13 68.2
MsG0580024071.01.T01 AT5G28530 29.282 181 119 4 18 192 56 233 6.65e-13 68.2
MsG0580024071.01.T01 AT3G59470 33.010 103 68 1 18 120 71 172 7.38e-13 66.6
MsG0580024071.01.T01 AT3G59470 33.010 103 68 1 18 120 71 172 7.38e-13 66.6
MsG0580024071.01.T01 AT3G59470 33.010 103 68 1 18 120 83 184 8.56e-13 66.6
MsG0580024071.01.T01 AT2G27110 46.429 56 30 0 191 246 51 106 4.04e-11 63.2

Find 45 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATTTGGAATTATTT+AGG 0.090273 5:+465880 MsG0580024071.01.T01:CDS
GTATACGAGTTACAAATATT+TGG 0.221426 5:+465869 MsG0580024071.01.T01:CDS
CAAATATTTGGAATTATTTA+GGG 0.245589 5:+465881 MsG0580024071.01.T01:CDS
CTCGTTTGACTAATGTCTTT+TGG 0.253639 5:+466082 MsG0580024071.01.T01:CDS
ATTATTCTTAAACCCTTCTT+TGG 0.257903 5:-465626 None:intergenic
AAGTTGATTATGATTGCTTT+GGG 0.313658 5:+466121 MsG0580024071.01.T01:CDS
AAAGTTGATTATGATTGCTT+TGG 0.341170 5:+466120 MsG0580024071.01.T01:CDS
CATGCCTTCAAATCAGGATT+TGG 0.352479 5:+465532 MsG0580024071.01.T01:CDS
ATGTTAGCAAGGAAGGAGTT+TGG 0.356508 5:+465719 MsG0580024071.01.T01:CDS
ACGAAACCTCATAAAGTCTA+TGG 0.362525 5:+465837 MsG0580024071.01.T01:CDS
TACACCAAATCCTGATTTGA+AGG 0.362762 5:-465536 None:intergenic
GGGTTTAAGAATAATGAATA+TGG 0.371754 5:+465634 MsG0580024071.01.T01:CDS
CAAGCACATGCCTTCAAATC+AGG 0.383509 5:+465526 MsG0580024071.01.T01:CDS
AACAGTAAAACTCTTTAAAA+CGG 0.389800 5:-465603 None:intergenic
AATTCACCATAGACTTTATG+AGG 0.389972 5:-465843 None:intergenic
TCAGGATTTGGTGTAAGAAA+AGG 0.407060 5:+465544 MsG0580024071.01.T01:CDS
AAGTTGAAATTGATTGAAGC+TGG 0.434164 5:+465973 MsG0580024071.01.T01:CDS
TGTCAATCAGTTGAAATTGA+TGG 0.438803 5:+465442 MsG0580024071.01.T01:CDS
AGGTTTAATGTTAGCAAGGA+AGG 0.442161 5:+465712 MsG0580024071.01.T01:CDS
ATAAAGTCTATGGTGAATTC+TGG 0.445432 5:+465847 MsG0580024071.01.T01:CDS
ACAGTAAAACTCTTTAAAAC+GGG 0.452807 5:-465602 None:intergenic
GACATTTGACATATTTCTCA+TGG 0.454279 5:-465812 None:intergenic
AGTTGAAATTGATTGAAGCT+GGG 0.466350 5:+465974 MsG0580024071.01.T01:CDS
GTCGGTGGATATAACAATCT+TGG 0.477476 5:+465910 MsG0580024071.01.T01:CDS
CAGGATTTGGTGTAAGAAAA+GGG 0.479534 5:+465545 MsG0580024071.01.T01:CDS
CTTATGAAAGAGCTGATTCA+AGG 0.480001 5:+465668 MsG0580024071.01.T01:CDS
CGCAACAAATGTCTTTGCTC+AGG 0.482031 5:-465787 None:intergenic
TTGGAATTATTTAGGGGAAG+AGG 0.485109 5:+465888 MsG0580024071.01.T01:CDS
TTACAATGTACAGCTAGATC+AGG 0.508809 5:+466056 MsG0580024071.01.T01:CDS
AAGGACAAATTGCAAAGCTA+TGG 0.528165 5:+465687 MsG0580024071.01.T01:CDS
ATTATGATCTAATACAAACT+TGG 0.563904 5:-465749 None:intergenic
TAATGAATATGGAGGTGAAG+TGG 0.572461 5:+465645 MsG0580024071.01.T01:CDS
GGTGAGGTTTAATGTTAGCA+AGG 0.579640 5:+465708 MsG0580024071.01.T01:CDS
TGTACATTGTAATAGAACAT+AGG 0.583397 5:-466045 None:intergenic
TATTTAGGGGAAGAGGTCGG+TGG 0.586341 5:+465895 MsG0580024071.01.T01:CDS
AATTATTTAGGGGAAGAGGT+CGG 0.592715 5:+465892 MsG0580024071.01.T01:CDS
GTTGAAATTGATTGAAGCTG+GGG 0.593382 5:+465975 MsG0580024071.01.T01:CDS
TTTAAGAATAATGAATATGG+AGG 0.604513 5:+465637 MsG0580024071.01.T01:CDS
CTGAGCAAAGACATTTGTTG+CGG 0.611498 5:+465788 MsG0580024071.01.T01:CDS
ATTTGGTGTAAGAAAAGGGA+GGG 0.620094 5:+465549 MsG0580024071.01.T01:CDS
CAAATTGCAAAGCTATGGTG+AGG 0.629755 5:+465692 MsG0580024071.01.T01:CDS
AAATATTTGGAATTATTTAG+GGG 0.632966 5:+465882 MsG0580024071.01.T01:CDS
AACAAATGTCTTTGCTCAGG+AGG 0.641459 5:-465784 None:intergenic
GATTTGGTGTAAGAAAAGGG+AGG 0.664580 5:+465548 MsG0580024071.01.T01:CDS
AGTTGATTATGATTGCTTTG+GGG 0.716100 5:+466122 MsG0580024071.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTTGGAATTATTTAG+GGG + Chr5:465882-465901 MsG0580024071.01.T01:CDS 15.0%
!!! ACAAATATTTGGAATTATTT+AGG + Chr5:465880-465899 MsG0580024071.01.T01:CDS 15.0%
!!! CAAATATTTGGAATTATTTA+GGG + Chr5:465881-465900 MsG0580024071.01.T01:CDS 15.0%
!! ATTATGATCTAATACAAACT+TGG - Chr5:465752-465771 None:intergenic 20.0%
!! TTTAAGAATAATGAATATGG+AGG + Chr5:465637-465656 MsG0580024071.01.T01:CDS 20.0%
!!! AACAGTAAAACTCTTTAAAA+CGG - Chr5:465606-465625 None:intergenic 20.0%
! ATTATTCTTAAACCCTTCTT+TGG - Chr5:465629-465648 None:intergenic 25.0%
! GGGTTTAAGAATAATGAATA+TGG + Chr5:465634-465653 MsG0580024071.01.T01:CDS 25.0%
! GTATACGAGTTACAAATATT+TGG + Chr5:465869-465888 MsG0580024071.01.T01:CDS 25.0%
! TGTACATTGTAATAGAACAT+AGG - Chr5:466048-466067 None:intergenic 25.0%
!! AAAGTTGATTATGATTGCTT+TGG + Chr5:466120-466139 MsG0580024071.01.T01:CDS 25.0%
!! AAGTTGATTATGATTGCTTT+GGG + Chr5:466121-466140 MsG0580024071.01.T01:CDS 25.0%
!!! ACAGTAAAACTCTTTAAAAC+GGG - Chr5:465605-465624 None:intergenic 25.0%
AAGTTGAAATTGATTGAAGC+TGG + Chr5:465973-465992 MsG0580024071.01.T01:CDS 30.0%
AATTCACCATAGACTTTATG+AGG - Chr5:465846-465865 None:intergenic 30.0%
AGTTGAAATTGATTGAAGCT+GGG + Chr5:465974-465993 MsG0580024071.01.T01:CDS 30.0%
AGTTGATTATGATTGCTTTG+GGG + Chr5:466122-466141 MsG0580024071.01.T01:CDS 30.0%
ATAAAGTCTATGGTGAATTC+TGG + Chr5:465847-465866 MsG0580024071.01.T01:CDS 30.0%
GACATTTGACATATTTCTCA+TGG - Chr5:465815-465834 None:intergenic 30.0%
TGTCAATCAGTTGAAATTGA+TGG + Chr5:465442-465461 MsG0580024071.01.T01:CDS 30.0%
! AGTTTTACTGTTCCAAAGAA+GGG + Chr5:465614-465633 MsG0580024071.01.T01:CDS 30.0%
! GGATGCAAAGATATTTTTCT+TGG + Chr5:465463-465482 MsG0580024071.01.T01:CDS 30.0%
AAGGACAAATTGCAAAGCTA+TGG + Chr5:465687-465706 MsG0580024071.01.T01:CDS 35.0%
AATTATTTAGGGGAAGAGGT+CGG + Chr5:465892-465911 MsG0580024071.01.T01:CDS 35.0%
ACGAAACCTCATAAAGTCTA+TGG + Chr5:465837-465856 MsG0580024071.01.T01:CDS 35.0%
AGGTTTAATGTTAGCAAGGA+AGG + Chr5:465712-465731 MsG0580024071.01.T01:CDS 35.0%
GTTGAAATTGATTGAAGCTG+GGG + Chr5:465975-465994 MsG0580024071.01.T01:CDS 35.0%
TAATGAATATGGAGGTGAAG+TGG + Chr5:465645-465664 MsG0580024071.01.T01:CDS 35.0%
TTACAATGTACAGCTAGATC+AGG + Chr5:466056-466075 MsG0580024071.01.T01:CDS 35.0%
TTGGAATTATTTAGGGGAAG+AGG + Chr5:465888-465907 MsG0580024071.01.T01:CDS 35.0%
! CTCGTTTGACTAATGTCTTT+TGG + Chr5:466082-466101 MsG0580024071.01.T01:CDS 35.0%
! CTTATGAAAGAGCTGATTCA+AGG + Chr5:465668-465687 MsG0580024071.01.T01:CDS 35.0%
! GAGTTTTACTGTTCCAAAGA+AGG + Chr5:465613-465632 MsG0580024071.01.T01:CDS 35.0%
! TACACCAAATCCTGATTTGA+AGG - Chr5:465539-465558 None:intergenic 35.0%
!! ACTAATGTCTTTTGGAGAGA+TGG + Chr5:466090-466109 MsG0580024071.01.T01:CDS 35.0%
!! ATTTGGTGTAAGAAAAGGGA+GGG + Chr5:465549-465568 MsG0580024071.01.T01:CDS 35.0%
!! CAGGATTTGGTGTAAGAAAA+GGG + Chr5:465545-465564 MsG0580024071.01.T01:CDS 35.0%
!! TCAGGATTTGGTGTAAGAAA+AGG + Chr5:465544-465563 MsG0580024071.01.T01:CDS 35.0%
AACAAATGTCTTTGCTCAGG+AGG - Chr5:465787-465806 None:intergenic 40.0%
ATGTTAGCAAGGAAGGAGTT+TGG + Chr5:465719-465738 MsG0580024071.01.T01:CDS 40.0%
CAAATTGCAAAGCTATGGTG+AGG + Chr5:465692-465711 MsG0580024071.01.T01:CDS 40.0%
CATGCCTTCAAATCAGGATT+TGG + Chr5:465532-465551 MsG0580024071.01.T01:CDS 40.0%
GGTGAGGTTTAATGTTAGCA+AGG + Chr5:465708-465727 MsG0580024071.01.T01:CDS 40.0%
GTCGGTGGATATAACAATCT+TGG + Chr5:465910-465929 MsG0580024071.01.T01:CDS 40.0%
! CTGAGCAAAGACATTTGTTG+CGG + Chr5:465788-465807 MsG0580024071.01.T01:CDS 40.0%
!! GATTTGGTGTAAGAAAAGGG+AGG + Chr5:465548-465567 MsG0580024071.01.T01:CDS 40.0%
CAAGCACATGCCTTCAAATC+AGG + Chr5:465526-465545 MsG0580024071.01.T01:CDS 45.0%
CGCAACAAATGTCTTTGCTC+AGG - Chr5:465790-465809 None:intergenic 45.0%
TATTTAGGGGAAGAGGTCGG+TGG + Chr5:465895-465914 MsG0580024071.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 465427 466170 465427 ID=MsG0580024071.01;Name=MsG0580024071.01
Chr5 mRNA 465427 466170 465427 ID=MsG0580024071.01.T01;Parent=MsG0580024071.01;Name=MsG0580024071.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|247
Chr5 exon 465427 466170 465427 ID=MsG0580024071.01.T01:exon:23063;Parent=MsG0580024071.01.T01
Chr5 CDS 465427 466170 465427 ID=MsG0580024071.01.T01:cds;Parent=MsG0580024071.01.T01
Gene Sequence

>MsG0580024071.01.T01

ATGGAGATAAATGAATGTCAATCAGTTGAAATTGATGGATGCAAAGATATTTTTCTTGGTCAAGTTGTGAATAACAAAGATGAAGCTTATAACTTATATCAAGCACATGCCTTCAAATCAGGATTTGGTGTAAGAAAAGGGAGGGAGTTATATTATGATAATGAGAGAAAAATCACCCGTTTTAAAGAGTTTTACTGTTCCAAAGAAGGGTTTAAGAATAATGAATATGGAGGTGAAGTGGCTTATGAAAGAGCTGATTCAAGGACAAATTGCAAAGCTATGGTGAGGTTTAATGTTAGCAAGGAAGGAGTTTGGAAAGTTACCAAGTTTGTATTAGATCATAATCATCAACTTGTACCTCCTGAGCAAAGACATTTGTTGCGGTCCATGAGAAATATGTCAAATGTCAAACGAAACCTCATAAAGTCTATGGTGAATTCTGGTATACGAGTTACAAATATTTGGAATTATTTAGGGGAAGAGGTCGGTGGATATAACAATCTTGGTATAACATTAAAAGATATGCATAATTATGTCTACACTGAAAAGTTGAAATTGATTGAAGCTGGGGATGCTCAATCTTTAGTAAATAACTTACAAAATAGACAAGCACAAGATCCTATGTTCTATTACAATGTACAGCTAGATCAGGAATCTCGTTTGACTAATGTCTTTTGGAGAGATGGTAAATCTAAAGTTGATTATGATTGCTTTGGGGATGTTGTGATATTTGACACTACTTAA

Protein sequence

>MsG0580024071.01.T01

MEINECQSVEIDGCKDIFLGQVVNNKDEAYNLYQAHAFKSGFGVRKGRELYYDNERKITRFKEFYCSKEGFKNNEYGGEVAYERADSRTNCKAMVRFNVSKEGVWKVTKFVLDHNHQLVPPEQRHLLRSMRNMSNVKRNLIKSMVNSGIRVTNIWNYLGEEVGGYNNLGITLKDMHNYVYTEKLKLIEAGDAQSLVNNLQNRQAQDPMFYYNVQLDQESRLTNVFWRDGKSKVDYDCFGDVVIFDTT*