Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024714.01.T01 | AES94499.1 | 92.994 | 157 | 11 | 0 | 1 | 157 | 1 | 157 | 8.59E-102 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024714.01.T01 | C0Z2L5 | 36.667 | 120 | 75 | 1 | 31 | 149 | 10 | 129 | 4.78E-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024714.01.T01 | G7K2E8 | 92.994 | 157 | 11 | 0 | 1 | 157 | 1 | 157 | 4.10e-102 | 298 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580024714.01.T01 | TF | bZIP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024714.01.T01 | MTR_5g015090 | 92.994 | 157 | 11 | 0 | 1 | 157 | 1 | 157 | 1.04e-105 | 298 |
| MsG0580024714.01.T01 | MTR_4g097440 | 54.598 | 174 | 62 | 6 | 1 | 157 | 1 | 174 | 6.90e-52 | 163 |
| MsG0580024714.01.T01 | MTR_3g103360 | 41.085 | 129 | 60 | 3 | 11 | 136 | 16 | 131 | 1.48e-16 | 72.0 |
| MsG0580024714.01.T01 | MTR_1g017570 | 53.086 | 81 | 38 | 0 | 60 | 140 | 59 | 139 | 3.81e-15 | 68.6 |
| MsG0580024714.01.T01 | MTR_7g029400 | 41.837 | 98 | 46 | 2 | 58 | 153 | 81 | 169 | 4.61e-14 | 66.6 |
| MsG0580024714.01.T01 | MTR_4g070860 | 68.182 | 44 | 14 | 0 | 58 | 101 | 27 | 70 | 6.92e-14 | 65.5 |
| MsG0580024714.01.T01 | MTR_8g015250 | 39.583 | 96 | 51 | 1 | 58 | 153 | 71 | 159 | 4.18e-13 | 63.9 |
| MsG0580024714.01.T01 | MTR_3g117120 | 37.895 | 95 | 51 | 1 | 58 | 144 | 27 | 121 | 5.91e-12 | 60.5 |
| MsG0580024714.01.T01 | MTR_8g086020 | 39.362 | 94 | 49 | 3 | 59 | 148 | 33 | 122 | 9.24e-12 | 60.1 |
| MsG0580024714.01.T01 | MTR_1g087040 | 36.667 | 90 | 42 | 2 | 36 | 125 | 8 | 82 | 1.06e-11 | 59.7 |
| MsG0580024714.01.T01 | MTR_1g022495 | 36.792 | 106 | 63 | 1 | 36 | 137 | 1 | 106 | 1.82e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024714.01.T01 | AT3G49760 | 38.129 | 139 | 85 | 1 | 5 | 142 | 15 | 153 | 1.96e-27 | 100 |
| MsG0580024714.01.T01 | AT4G37730 | 43.609 | 133 | 60 | 3 | 7 | 124 | 135 | 267 | 3.92e-19 | 82.0 |
| MsG0580024714.01.T01 | AT2G22850 | 47.059 | 85 | 45 | 0 | 59 | 143 | 127 | 211 | 3.12e-18 | 78.6 |
| MsG0580024714.01.T01 | AT2G22850 | 47.059 | 85 | 45 | 0 | 59 | 143 | 127 | 211 | 3.12e-18 | 78.6 |
| MsG0580024714.01.T01 | AT3G30530 | 40.909 | 110 | 63 | 1 | 27 | 136 | 49 | 156 | 1.18e-14 | 67.8 |
| MsG0580024714.01.T01 | AT1G75390 | 36.667 | 120 | 75 | 1 | 31 | 149 | 10 | 129 | 4.88e-14 | 66.2 |
| MsG0580024714.01.T01 | AT2G18160 | 35.577 | 104 | 60 | 1 | 52 | 148 | 15 | 118 | 9.41e-13 | 62.8 |
| MsG0580024714.01.T01 | AT4G34590 | 40.449 | 89 | 53 | 0 | 58 | 146 | 24 | 112 | 1.07e-12 | 62.4 |
| MsG0580024714.01.T01 | AT1G75390 | 38.384 | 99 | 60 | 1 | 31 | 128 | 10 | 108 | 2.27e-12 | 60.8 |
| MsG0580024714.01.T01 | AT1G13600 | 45.679 | 81 | 37 | 1 | 58 | 138 | 83 | 156 | 5.16e-12 | 61.2 |
| MsG0580024714.01.T01 | AT4G36730 | 33.628 | 113 | 56 | 2 | 48 | 141 | 194 | 306 | 2.08e-11 | 60.8 |
| MsG0580024714.01.T01 | AT4G36730 | 33.628 | 113 | 56 | 2 | 48 | 141 | 192 | 304 | 2.21e-11 | 60.8 |
| MsG0580024714.01.T01 | AT2G04038 | 50.000 | 70 | 30 | 1 | 36 | 105 | 54 | 118 | 2.28e-11 | 58.9 |
| MsG0580024714.01.T01 | AT5G15830 | 47.945 | 73 | 32 | 1 | 33 | 105 | 52 | 118 | 5.08e-11 | 58.5 |
Find 53 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACATCTAGAGAATTTA+CGG | 0.286559 | 5:-8695769 | MsG0580024714.01.T01:CDS |
| GGATGGCTCGAACCGGAATA+TGG | 0.309967 | 5:-8695858 | MsG0580024714.01.T01:CDS |
| GGGCTGGTGGGTTGGAAGGG+TGG | 0.313437 | 5:+8695932 | None:intergenic |
| ATATCATTCTCCTCCGCTTT+CGG | 0.329484 | 5:+8695831 | None:intergenic |
| GCAACCCGTTGTTCAATTCC+CGG | 0.358900 | 5:+8695717 | None:intergenic |
| CAACCGGAGCCACTCACTTT+CGG | 0.360505 | 5:+8695661 | None:intergenic |
| GTGACCTCCGTGCCGATTCC+CGG | 0.363167 | 5:+8695804 | None:intergenic |
| TTCTTCTAACTCCGTGAAAT+CGG | 0.384933 | 5:+8695988 | None:intergenic |
| GCTTTCGGTCCTCCATATTC+CGG | 0.399400 | 5:+8695846 | None:intergenic |
| CTTAGGGCTGGTGGGTTGGA+AGG | 0.408872 | 5:+8695928 | None:intergenic |
| ACCGGTTTAGAGTTGAAAAC+CGG | 0.409929 | 5:-8695736 | MsG0580024714.01.T01:CDS |
| CCGGTTGGAACGAACCATGC+TGG | 0.411515 | 5:-8695645 | MsG0580024714.01.T01:CDS |
| TCTCTATGCCTCCGATTTCA+CGG | 0.417032 | 5:-8695999 | MsG0580024714.01.T01:CDS |
| CTCACTTCAAGCTCCGGTTC+AGG | 0.426863 | 5:-8695905 | MsG0580024714.01.T01:CDS |
| CCGGAGCTTGAAGTGAGCTT+AGG | 0.429061 | 5:+8695911 | None:intergenic |
| AAAACCGGGAATTGAACAAC+GGG | 0.452979 | 5:-8695721 | MsG0580024714.01.T01:CDS |
| ATTTACGGAACCAGGTGAAC+CGG | 0.455271 | 5:-8695754 | MsG0580024714.01.T01:CDS |
| CCAGCATGGTTCGTTCCAAC+CGG | 0.461195 | 5:+8695645 | None:intergenic |
| TGAAGTGAGCTTAGGGCTGG+TGG | 0.471435 | 5:+8695919 | None:intergenic |
| CAACTCTAAACCGGTTCACC+TGG | 0.478647 | 5:+8695744 | None:intergenic |
| CGGAGCTTGAAGTGAGCTTA+GGG | 0.479442 | 5:+8695912 | None:intergenic |
| TTAGGGCTGGTGGGTTGGAA+GGG | 0.484445 | 5:+8695929 | None:intergenic |
| CGGTCATGGATGGCTCGAAC+CGG | 0.489104 | 5:-8695865 | MsG0580024714.01.T01:CDS |
| CGGTTCGAGCCATCCATGAC+CGG | 0.490515 | 5:+8695866 | None:intergenic |
| GGAGGAGAATGATATCAAAC+CGG | 0.491685 | 5:-8695823 | MsG0580024714.01.T01:CDS |
| TTCAACCTTCCTCATCTGCA+TGG | 0.493496 | 5:-8695586 | MsG0580024714.01.T01:CDS |
| AATGATATCAAACCGGGAAT+CGG | 0.493933 | 5:-8695816 | MsG0580024714.01.T01:CDS |
| GAACCGAAAGTGAGTGGCTC+CGG | 0.506432 | 5:-8695664 | MsG0580024714.01.T01:CDS |
| TGAGCTTAGGGCTGGTGGGT+TGG | 0.515513 | 5:+8695924 | None:intergenic |
| CCTAAGCTCACTTCAAGCTC+CGG | 0.518730 | 5:-8695911 | MsG0580024714.01.T01:CDS |
| CGGTTGGAACGAACCATGCT+GGG | 0.526524 | 5:-8695644 | MsG0580024714.01.T01:CDS |
| GAAGTGAGCTTAGGGCTGGT+GGG | 0.547493 | 5:+8695920 | None:intergenic |
| GGCATAGAGAGAAGAGAGCA+TGG | 0.571054 | 5:+8696012 | None:intergenic |
| GCTTGAAGTGAGCTTAGGGC+TGG | 0.571567 | 5:+8695916 | None:intergenic |
| ATTGCATGGCCATGCAGATG+AGG | 0.577745 | 5:+8695577 | None:intergenic |
| AGAAGAAAGTTTAACGCCGT+GGG | 0.579656 | 5:-8695972 | MsG0580024714.01.T01:CDS |
| CCGGTTTAGAGTTGAAAACC+GGG | 0.582456 | 5:-8695735 | MsG0580024714.01.T01:CDS |
| TCTAGAGAATTTACGGAACC+AGG | 0.588686 | 5:-8695762 | MsG0580024714.01.T01:CDS |
| TAGAAGAAAGTTTAACGCCG+TGG | 0.593173 | 5:-8695973 | MsG0580024714.01.T01:CDS |
| CGAAAGTGAGTGGCTCCGGT+TGG | 0.593366 | 5:-8695660 | MsG0580024714.01.T01:CDS |
| TATCAAACCGGGAATCGGCA+CGG | 0.599707 | 5:-8695811 | MsG0580024714.01.T01:CDS |
| GTCTACGAACCGAAAGTGAG+TGG | 0.602935 | 5:-8695670 | MsG0580024714.01.T01:CDS |
| TTCTAACTCCGTGAAATCGG+AGG | 0.604958 | 5:+8695991 | None:intergenic |
| GGTCAAAAGTTGTCAAACAT+AGG | 0.610074 | 5:-8695623 | MsG0580024714.01.T01:CDS |
| GGAATATGGAGGACCGAAAG+CGG | 0.611264 | 5:-8695844 | MsG0580024714.01.T01:CDS |
| GAGGAGAATGATATCAAACC+GGG | 0.616694 | 5:-8695822 | MsG0580024714.01.T01:CDS |
| CATGGCCATGCAGATGAGGA+AGG | 0.636096 | 5:+8695581 | None:intergenic |
| TCAGCTGTGACAATATTGCA+TGG | 0.636378 | 5:+8695563 | None:intergenic |
| GTCAAACATAGGTCAAAACA+TGG | 0.654401 | 5:-8695612 | MsG0580024714.01.T01:CDS |
| GAAAACCGGGAATTGAACAA+CGG | 0.656607 | 5:-8695722 | MsG0580024714.01.T01:CDS |
| ATATGGAGGACCGAAAGCGG+AGG | 0.670255 | 5:-8695841 | MsG0580024714.01.T01:CDS |
| TGGCTCGAACCGGAATATGG+AGG | 0.676236 | 5:-8695855 | MsG0580024714.01.T01:CDS |
| CAAACCGGGAATCGGCACGG+AGG | 0.765542 | 5:-8695808 | MsG0580024714.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGACATCTAGAGAATTTA+CGG | - | Chr5:8695800-8695819 | MsG0580024714.01.T01:CDS | 25.0% |
| AGAATGTTAGAAAAATCCCA+CGG | + | Chr5:8695616-8695635 | None:intergenic | 30.0% | |
| AATGATATCAAACCGGGAAT+CGG | - | Chr5:8695753-8695772 | MsG0580024714.01.T01:CDS | 35.0% | |
| GGTCAAAAGTTGTCAAACAT+AGG | - | Chr5:8695946-8695965 | MsG0580024714.01.T01:CDS | 35.0% | |
| GTCAAACATAGGTCAAAACA+TGG | - | Chr5:8695957-8695976 | MsG0580024714.01.T01:CDS | 35.0% | |
| TTCTTCTAACTCCGTGAAAT+CGG | + | Chr5:8695584-8695603 | None:intergenic | 35.0% | |
| AAAACCGGGAATTGAACAAC+GGG | - | Chr5:8695848-8695867 | MsG0580024714.01.T01:CDS | 40.0% | |
| AAAGAAAAACCGGTCATGGA+TGG | - | Chr5:8695694-8695713 | MsG0580024714.01.T01:CDS | 40.0% | |
| ACCAAAAGAAAAACCGGTCA+TGG | - | Chr5:8695690-8695709 | MsG0580024714.01.T01:CDS | 40.0% | |
| AGAAGAAAGTTTAACGCCGT+GGG | - | Chr5:8695597-8695616 | MsG0580024714.01.T01:CDS | 40.0% | |
| AGGCGAACCAAAAGAAAAAC+CGG | - | Chr5:8695684-8695703 | MsG0580024714.01.T01:CDS | 40.0% | |
| ATATCATTCTCCTCCGCTTT+CGG | + | Chr5:8695741-8695760 | None:intergenic | 40.0% | |
| GAAAACCGGGAATTGAACAA+CGG | - | Chr5:8695847-8695866 | MsG0580024714.01.T01:CDS | 40.0% | |
| GAGGAGAATGATATCAAACC+GGG | - | Chr5:8695747-8695766 | MsG0580024714.01.T01:CDS | 40.0% | |
| GGAGGAGAATGATATCAAAC+CGG | - | Chr5:8695746-8695765 | MsG0580024714.01.T01:CDS | 40.0% | |
| TAGAAGAAAGTTTAACGCCG+TGG | - | Chr5:8695596-8695615 | MsG0580024714.01.T01:CDS | 40.0% | |
| TCAGCTGTGACAATATTGCA+TGG | + | Chr5:8696009-8696028 | None:intergenic | 40.0% | |
| TCTAGAGAATTTACGGAACC+AGG | - | Chr5:8695807-8695826 | MsG0580024714.01.T01:CDS | 40.0% | |
| ! | ACCGGTTTAGAGTTGAAAAC+CGG | - | Chr5:8695833-8695852 | MsG0580024714.01.T01:CDS | 40.0% |
| !! | TCCATGACCGGTTTTTCTTT+TGG | + | Chr5:8695694-8695713 | None:intergenic | 40.0% |
| ATTTACGGAACCAGGTGAAC+CGG | - | Chr5:8695815-8695834 | MsG0580024714.01.T01:CDS | 45.0% | |
| TCTCTATGCCTCCGATTTCA+CGG | - | Chr5:8695570-8695589 | MsG0580024714.01.T01:CDS | 45.0% | |
| TTCAACCTTCCTCATCTGCA+TGG | - | Chr5:8695983-8696002 | MsG0580024714.01.T01:CDS | 45.0% | |
| TTCTAACTCCGTGAAATCGG+AGG | + | Chr5:8695581-8695600 | None:intergenic | 45.0% | |
| ! | CCCGGTTTTCAACTCTAAAC+CGG | + | Chr5:8695837-8695856 | None:intergenic | 45.0% |
| ! | CCGGTTTAGAGTTGAAAACC+GGG | - | Chr5:8695834-8695853 | MsG0580024714.01.T01:CDS | 45.0% |
| ! | TGACAACTTTTGACCCAGCA+TGG | + | Chr5:8695941-8695960 | None:intergenic | 45.0% |
| !!! | TTCTTTTGGTTCGCCTGAAC+CGG | + | Chr5:8695680-8695699 | None:intergenic | 45.0% |
| ATTGCATGGCCATGCAGATG+AGG | + | Chr5:8695995-8696014 | None:intergenic | 50.0% | |
| CCTAAGCTCACTTCAAGCTC+CGG | - | Chr5:8695658-8695677 | MsG0580024714.01.T01:CDS | 50.0% | |
| CGGAGCTTGAAGTGAGCTTA+GGG | + | Chr5:8695660-8695679 | None:intergenic | 50.0% | |
| GCAACCCGTTGTTCAATTCC+CGG | + | Chr5:8695855-8695874 | None:intergenic | 50.0% | |
| GCTTTCGGTCCTCCATATTC+CGG | + | Chr5:8695726-8695745 | None:intergenic | 50.0% | |
| GGAATATGGAGGACCGAAAG+CGG | - | Chr5:8695725-8695744 | MsG0580024714.01.T01:CDS | 50.0% | |
| GTCTACGAACCGAAAGTGAG+TGG | - | Chr5:8695899-8695918 | MsG0580024714.01.T01:CDS | 50.0% | |
| TATCAAACCGGGAATCGGCA+CGG | - | Chr5:8695758-8695777 | MsG0580024714.01.T01:CDS | 50.0% | |
| ! | CAACTCTAAACCGGTTCACC+TGG | + | Chr5:8695828-8695847 | None:intergenic | 50.0% |
| ATATGGAGGACCGAAAGCGG+AGG | - | Chr5:8695728-8695747 | MsG0580024714.01.T01:CDS | 55.0% | |
| CAACCGGAGCCACTCACTTT+CGG | + | Chr5:8695911-8695930 | None:intergenic | 55.0% | |
| CATGGCCATGCAGATGAGGA+AGG | + | Chr5:8695991-8696010 | None:intergenic | 55.0% | |
| CCAGCATGGTTCGTTCCAAC+CGG | + | Chr5:8695927-8695946 | None:intergenic | 55.0% | |
| CCGGAGCTTGAAGTGAGCTT+AGG | + | Chr5:8695661-8695680 | None:intergenic | 55.0% | |
| CGGTTGGAACGAACCATGCT+GGG | - | Chr5:8695925-8695944 | MsG0580024714.01.T01:CDS | 55.0% | |
| CTCACTTCAAGCTCCGGTTC+AGG | - | Chr5:8695664-8695683 | MsG0580024714.01.T01:CDS | 55.0% | |
| GAACCGAAAGTGAGTGGCTC+CGG | - | Chr5:8695905-8695924 | MsG0580024714.01.T01:CDS | 55.0% | |
| GCTTGAAGTGAGCTTAGGGC+TGG | + | Chr5:8695656-8695675 | None:intergenic | 55.0% | |
| GGATGGCTCGAACCGGAATA+TGG | - | Chr5:8695711-8695730 | MsG0580024714.01.T01:CDS | 55.0% | |
| TGAAGTGAGCTTAGGGCTGG+TGG | + | Chr5:8695653-8695672 | None:intergenic | 55.0% | |
| TGGCTCGAACCGGAATATGG+AGG | - | Chr5:8695714-8695733 | MsG0580024714.01.T01:CDS | 55.0% | |
| ! | GAAGTGAGCTTAGGGCTGGT+GGG | + | Chr5:8695652-8695671 | None:intergenic | 55.0% |
| !! | TTAGGGCTGGTGGGTTGGAA+GGG | + | Chr5:8695643-8695662 | None:intergenic | 55.0% |
| CCGGTTGGAACGAACCATGC+TGG | - | Chr5:8695924-8695943 | MsG0580024714.01.T01:CDS | 60.0% | |
| CGAAAGTGAGTGGCTCCGGT+TGG | - | Chr5:8695909-8695928 | MsG0580024714.01.T01:CDS | 60.0% | |
| CGGTCATGGATGGCTCGAAC+CGG | - | Chr5:8695704-8695723 | MsG0580024714.01.T01:CDS | 60.0% | |
| CGGTTCGAGCCATCCATGAC+CGG | + | Chr5:8695706-8695725 | None:intergenic | 60.0% | |
| ! | TGAGCTTAGGGCTGGTGGGT+TGG | + | Chr5:8695648-8695667 | None:intergenic | 60.0% |
| !! | CTTAGGGCTGGTGGGTTGGA+AGG | + | Chr5:8695644-8695663 | None:intergenic | 60.0% |
| CAAACCGGGAATCGGCACGG+AGG | - | Chr5:8695761-8695780 | MsG0580024714.01.T01:CDS | 65.0% | |
| !! | GTGACCTCCGTGCCGATTCC+CGG | + | Chr5:8695768-8695787 | None:intergenic | 65.0% |
| !! | GGGCTGGTGGGTTGGAAGGG+TGG | + | Chr5:8695640-8695659 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 8695559 | 8696032 | 8695559 | ID=MsG0580024714.01;Name=MsG0580024714.01 |
| Chr5 | mRNA | 8695559 | 8696032 | 8695559 | ID=MsG0580024714.01.T01;Parent=MsG0580024714.01;Name=MsG0580024714.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|157 |
| Chr5 | exon | 8695559 | 8696032 | 8695559 | ID=MsG0580024714.01.T01:exon:19496;Parent=MsG0580024714.01.T01 |
| Chr5 | CDS | 8695559 | 8696032 | 8695559 | ID=MsG0580024714.01.T01:cds;Parent=MsG0580024714.01.T01 |
| Gene Sequence |
| Protein sequence |
