AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024817.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024817.01.T01 MTR_5g017140 77.397 146 26 4 1 143 133 274 3.97e-70 213
MsG0580024817.01.T01 MTR_5g017110 59.459 148 51 4 1 143 134 277 1.23e-45 152
MsG0580024817.01.T01 MTR_0189s0010 57.042 142 52 4 1 138 151 287 3.98e-42 143
MsG0580024817.01.T01 MTR_0154s0020 55.634 142 54 4 1 138 131 267 1.22e-40 139
MsG0580024817.01.T01 MTR_0154s0040 55.319 141 54 4 1 137 131 266 6.23e-40 137
MsG0580024817.01.T01 MTR_8g098425 39.695 131 73 3 3 131 538 664 4.70e-22 91.3
MsG0580024817.01.T01 MTR_1g100250 38.168 131 75 3 3 131 541 667 3.17e-21 89.0
MsG0580024817.01.T01 MTR_3g096230 36.090 133 79 3 3 133 374 502 4.10e-21 88.6
MsG0580024817.01.T01 MTR_3g096230 36.090 133 79 3 3 133 374 502 6.21e-21 88.2
MsG0580024817.01.T01 MTR_2g023890 41.176 136 70 6 3 136 132 259 8.68e-21 86.7
MsG0580024817.01.T01 MTR_2g005290 30.667 150 83 3 3 131 139 288 1.00e-18 81.3
MsG0580024817.01.T01 MTR_2g088020 41.985 131 66 5 3 131 134 256 1.13e-18 81.3
MsG0580024817.01.T01 MTR_8g093730 34.586 133 81 3 3 133 429 557 2.09e-18 80.9
MsG0580024817.01.T01 MTR_7g113490 34.586 133 83 3 3 132 201 332 3.19e-18 80.5
MsG0580024817.01.T01 MTR_3g073250 37.121 132 74 3 3 131 398 523 3.77e-18 80.1
MsG0580024817.01.T01 MTR_4g049730 38.060 134 74 5 3 134 126 252 4.54e-18 79.7
MsG0580024817.01.T01 MTR_5g059880 37.879 132 73 4 3 131 320 445 7.69e-18 79.0
MsG0580024817.01.T01 MTR_5g059940 36.090 133 76 3 3 132 253 379 3.16e-17 77.0
MsG0580024817.01.T01 MTR_3g101210 31.757 148 83 5 3 140 438 577 5.08e-17 77.0
MsG0580024817.01.T01 MTR_3g101210 31.757 148 83 5 3 140 438 577 5.42e-17 76.6
MsG0580024817.01.T01 MTR_5g060350 36.364 132 75 3 3 131 449 574 5.50e-17 76.6
MsG0580024817.01.T01 MTR_0685s0020 37.405 131 73 3 3 130 119 243 5.60e-17 75.5
MsG0580024817.01.T01 MTR_5g017890 35.211 142 78 5 3 136 503 638 7.05e-17 76.6
MsG0580024817.01.T01 MTR_4g094415 37.121 132 74 4 3 131 292 417 1.99e-16 75.1
MsG0580024817.01.T01 MTR_1g105290 35.338 133 82 3 3 132 188 319 2.02e-16 75.1
MsG0580024817.01.T01 MTR_1g105290 35.338 133 82 3 3 132 188 319 2.11e-16 75.1
MsG0580024817.01.T01 MTR_1g105290 35.338 133 82 3 3 132 188 319 3.22e-16 74.7
MsG0580024817.01.T01 MTR_5g071560 41.667 120 63 4 13 131 150 263 6.06e-14 67.8
MsG0580024817.01.T01 MTR_4g074875 33.088 136 86 3 3 137 149 280 1.19e-12 64.3
MsG0580024817.01.T01 MTR_4g074875 33.088 136 86 3 3 137 149 280 1.26e-12 64.3
MsG0580024817.01.T01 MTR_5g059910 33.835 133 79 3 3 132 259 385 3.31e-12 62.8
MsG0580024817.01.T01 MTR_4g075410 32.576 132 84 3 3 133 149 276 3.79e-12 63.2
MsG0580024817.01.T01 MTR_4g075410 32.576 132 84 3 3 133 149 276 3.86e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024817.01.T01 AT3G50310 48.322 149 56 6 1 136 134 274 3.18e-34 122
MsG0580024817.01.T01 AT5G67080 47.020 151 66 6 1 142 136 281 1.05e-33 120
MsG0580024817.01.T01 AT4G36950 47.826 138 61 4 1 132 134 266 2.08e-31 114
MsG0580024817.01.T01 AT1G05100 42.336 137 70 4 3 135 136 267 3.05e-23 93.2
MsG0580024817.01.T01 AT1G07150 38.686 137 78 4 3 138 141 272 1.32e-21 89.7
MsG0580024817.01.T01 AT1G07150 38.686 137 78 4 3 138 155 286 1.36e-21 89.7
MsG0580024817.01.T01 AT4G26890 40.000 135 71 4 3 135 131 257 1.91e-21 89.4
MsG0580024817.01.T01 AT5G55090 42.748 131 68 4 13 141 141 266 1.92e-21 89.4
MsG0580024817.01.T01 AT5G55090 42.748 131 68 4 13 141 203 328 2.99e-21 89.0
MsG0580024817.01.T01 AT2G32510 38.621 145 76 5 3 139 131 270 2.91e-20 85.5
MsG0580024817.01.T01 AT2G30040 40.690 145 77 6 3 144 145 283 4.68e-20 85.5
MsG0580024817.01.T01 AT1G63700 36.567 134 79 3 3 134 530 659 6.98e-20 85.1
MsG0580024817.01.T01 AT1G63700 36.567 134 79 3 3 134 530 659 6.98e-20 85.1
MsG0580024817.01.T01 AT1G54960 34.028 144 92 3 3 144 201 343 2.27e-18 80.9
MsG0580024817.01.T01 AT1G54960 34.028 144 92 3 3 144 201 343 2.27e-18 80.9
MsG0580024817.01.T01 AT1G53570 33.333 138 86 3 3 138 344 477 2.50e-18 80.9
MsG0580024817.01.T01 AT1G53570 33.333 138 86 3 3 138 344 477 2.50e-18 80.9
MsG0580024817.01.T01 AT1G53570 33.333 138 86 3 3 138 344 477 2.50e-18 80.5
MsG0580024817.01.T01 AT1G53570 33.333 138 86 3 3 138 344 477 2.52e-18 80.9
MsG0580024817.01.T01 AT1G53570 33.333 138 86 3 3 138 344 477 2.52e-18 80.9
MsG0580024817.01.T01 AT1G53570 33.333 138 86 3 3 138 344 477 2.58e-18 80.5
MsG0580024817.01.T01 AT1G09000 35.664 143 89 3 3 143 202 343 9.25e-18 79.0
MsG0580024817.01.T01 AT1G09000 35.664 143 89 3 3 143 202 343 1.07e-17 79.0
MsG0580024817.01.T01 AT3G46140 40.650 123 68 4 13 131 248 369 2.15e-17 77.8
MsG0580024817.01.T01 AT3G06030 34.091 132 84 3 3 132 201 331 4.82e-17 77.0
MsG0580024817.01.T01 AT4G12020 34.815 135 73 3 3 132 1679 1803 1.45e-16 75.9
MsG0580024817.01.T01 AT4G12020 34.815 135 73 3 3 132 1754 1878 1.45e-16 75.9
MsG0580024817.01.T01 AT4G12020 34.815 135 73 3 3 132 1746 1870 1.48e-16 75.9
MsG0580024817.01.T01 AT3G46160 37.313 134 76 5 4 131 201 332 7.94e-16 73.2
MsG0580024817.01.T01 AT4G12020 34.615 130 64 3 3 132 1754 1862 1.87e-15 72.4
MsG0580024817.01.T01 AT4G12020 34.615 130 64 3 3 132 1746 1854 1.95e-15 72.4
MsG0580024817.01.T01 AT5G66850 31.206 141 79 5 3 133 477 609 1.33e-14 70.1
MsG0580024817.01.T01 AT5G66850 31.206 141 79 5 3 133 477 609 1.55e-14 69.7
MsG0580024817.01.T01 AT4G08500 36.090 133 75 4 3 131 461 587 1.82e-14 69.7
MsG0580024817.01.T01 AT1G53570 32.653 98 64 1 43 138 354 451 9.51e-14 67.4
MsG0580024817.01.T01 AT3G45670 35.338 133 75 4 13 138 244 372 1.74e-13 66.6
MsG0580024817.01.T01 AT4G04632 39.796 98 54 3 43 135 1 98 4.09e-13 62.0
MsG0580024817.01.T01 AT4G08480 32.143 140 69 4 4 131 630 755 1.24e-12 64.3
MsG0580024817.01.T01 AT4G08470 30.714 140 73 4 3 131 349 475 2.26e-12 63.5
MsG0580024817.01.T01 AT4G08470 30.714 140 73 4 3 131 431 557 2.93e-12 63.2
MsG0580024817.01.T01 AT3G13530 32.576 132 84 3 3 133 149 276 4.54e-12 62.8
MsG0580024817.01.T01 AT3G07980 31.818 132 85 4 3 133 149 276 1.04e-11 62.0
MsG0580024817.01.T01 AT3G07980 31.818 132 85 4 3 133 149 276 1.19e-11 61.6
MsG0580024817.01.T01 AT2G42550 34.307 137 77 6 3 131 143 274 7.07e-11 58.9
MsG0580024817.01.T01 AT2G41930 33.577 137 67 6 12 136 157 281 8.62e-11 58.9

Find 36 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATCCATATCACCGCTTC+TGG 0.337412 5:+10173382 None:intergenic
TGTCACCAGAAGCGGTGATA+TGG 0.351676 5:-10173387 MsG0580024817.01.T01:CDS
GTGTGTGGAAGACGGATATA+TGG 0.363357 5:-10173360 MsG0580024817.01.T01:CDS
CCTTCTCGTAATAATTCATC+TGG 0.379686 5:+10173226 None:intergenic
TCTGGTGACATATCATTATC+TGG 0.395860 5:+10173400 None:intergenic
TTAAGGATCCTTTGAGAAGA+TGG 0.405417 5:-10173177 MsG0580024817.01.T01:CDS
TGATGTTGACGATTCGAATT+GGG 0.421148 5:-10173273 MsG0580024817.01.T01:CDS
TCTTGAAAAGTGTTTCGTTA+AGG 0.422240 5:-10173194 MsG0580024817.01.T01:CDS
TTGATGTTGACGATTCGAAT+TGG 0.426586 5:-10173274 MsG0580024817.01.T01:CDS
TTCTCGAATACGAGAATGTT+GGG 0.438025 5:+10173511 None:intergenic
GCTAGTGTGGAAATGGTTAC+TGG 0.449239 5:-10173325 MsG0580024817.01.T01:CDS
TGACATATCATTATCTGGTC+CGG 0.454822 5:+10173405 None:intergenic
TTGACGATTCGAATTGGGGT+TGG 0.467699 5:-10173268 MsG0580024817.01.T01:CDS
TGGTTGCGCTAGTGTGGAAA+TGG 0.477249 5:-10173332 MsG0580024817.01.T01:CDS
AACATTCTCGTATTCGAGAA+TGG 0.492631 5:-10173508 MsG0580024817.01.T01:CDS
CAGATGAATTATTACGAGAA+GGG 0.520167 5:-10173225 MsG0580024817.01.T01:CDS
GACATATCATTATCTGGTCC+GGG 0.525671 5:+10173406 None:intergenic
CCAGATGAATTATTACGAGA+AGG 0.525954 5:-10173226 MsG0580024817.01.T01:CDS
AAGACGGATATATGGGCTCT+TGG 0.538128 5:-10173352 MsG0580024817.01.T01:CDS
ATTCTCGAATACGAGAATGT+TGG 0.543098 5:+10173510 None:intergenic
GGCTCTTGGTTGCGCTAGTG+TGG 0.543527 5:-10173338 MsG0580024817.01.T01:CDS
TGGCGATCAAGAGGAACTCC+CGG 0.556693 5:-10173424 MsG0580024817.01.T01:CDS
CGGTGATATGGATGTGTGTG+TGG 0.571269 5:-10173375 MsG0580024817.01.T01:CDS
GGTGAAAAGTGGCGATCAAG+AGG 0.575193 5:-10173433 MsG0580024817.01.T01:CDS
TAATGATATGTCACCAGAAG+CGG 0.578864 5:-10173395 MsG0580024817.01.T01:CDS
TGTGTGGAAGACGGATATAT+GGG 0.584811 5:-10173359 MsG0580024817.01.T01:CDS
GTTGTAACAGAGTTAAGTTG+AGG 0.584853 5:-10173100 None:intergenic
ATGGATGTGTGTGTGGAAGA+CGG 0.590089 5:-10173368 MsG0580024817.01.T01:CDS
GGGTTGAACAAGGTGAAAAG+TGG 0.592877 5:-10173444 MsG0580024817.01.T01:CDS
TCAGTCTTGCGAAAGAGACA+AGG 0.595495 5:-10173465 MsG0580024817.01.T01:CDS
GATGTTGACGATTCGAATTG+GGG 0.607028 5:-10173272 MsG0580024817.01.T01:CDS
TCTATCGTCCATCTTCTCAA+AGG 0.609523 5:+10173169 None:intergenic
CAGTCTTGCGAAAGAGACAA+GGG 0.612424 5:-10173464 MsG0580024817.01.T01:CDS
TGGTTACTGGAGAATCCGCA+TGG 0.636768 5:-10173312 MsG0580024817.01.T01:CDS
AAAGAGACAAGGGTTGAACA+AGG 0.656891 5:-10173454 MsG0580024817.01.T01:CDS
CATATCTGACACATTCCATG+CGG 0.745519 5:+10173297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATTCATCTGGAATTTT+TGG + Chr5:10173384-10173403 None:intergenic 20.0%
!! AACTGAAACATTTTCAACAT+CGG + Chr5:10173499-10173518 None:intergenic 25.0%
CAGATGAATTATTACGAGAA+GGG - Chr5:10173391-10173410 MsG0580024817.01.T01:CDS 30.0%
! TTTTCAACATCGGAGATAAA+CGG + Chr5:10173489-10173508 None:intergenic 30.0%
!! TCTTGAAAAGTGTTTCGTTA+AGG - Chr5:10173422-10173441 MsG0580024817.01.T01:CDS 30.0%
AACATTCTCGTATTCGAGAA+TGG - Chr5:10173108-10173127 MsG0580024817.01.T01:CDS 35.0%
ATTCTCGAATACGAGAATGT+TGG + Chr5:10173109-10173128 None:intergenic 35.0%
CCAGATGAATTATTACGAGA+AGG - Chr5:10173390-10173409 MsG0580024817.01.T01:CDS 35.0%
CCTTCTCGTAATAATTCATC+TGG + Chr5:10173393-10173412 None:intergenic 35.0%
TAATGATATGTCACCAGAAG+CGG - Chr5:10173221-10173240 MsG0580024817.01.T01:CDS 35.0%
TCTGGTGACATATCATTATC+TGG + Chr5:10173219-10173238 None:intergenic 35.0%
TGACATATCATTATCTGGTC+CGG + Chr5:10173214-10173233 None:intergenic 35.0%
TGATGTTGACGATTCGAATT+GGG - Chr5:10173343-10173362 MsG0580024817.01.T01:CDS 35.0%
TTCTCGAATACGAGAATGTT+GGG + Chr5:10173108-10173127 None:intergenic 35.0%
TTGATGTTGACGATTCGAAT+TGG - Chr5:10173342-10173361 MsG0580024817.01.T01:CDS 35.0%
! TTAAGGATCCTTTGAGAAGA+TGG - Chr5:10173439-10173458 MsG0580024817.01.T01:CDS 35.0%
AAAGAGACAAGGGTTGAACA+AGG - Chr5:10173162-10173181 MsG0580024817.01.T01:CDS 40.0%
CATATCTGACACATTCCATG+CGG + Chr5:10173322-10173341 None:intergenic 40.0%
GACATATCATTATCTGGTCC+GGG + Chr5:10173213-10173232 None:intergenic 40.0%
GATGTTGACGATTCGAATTG+GGG - Chr5:10173344-10173363 MsG0580024817.01.T01:CDS 40.0%
TCTATCGTCCATCTTCTCAA+AGG + Chr5:10173450-10173469 None:intergenic 40.0%
TGTGTGGAAGACGGATATAT+GGG - Chr5:10173257-10173276 MsG0580024817.01.T01:CDS 40.0%
AAGACGGATATATGGGCTCT+TGG - Chr5:10173264-10173283 MsG0580024817.01.T01:CDS 45.0%
ATGGATGTGTGTGTGGAAGA+CGG - Chr5:10173248-10173267 MsG0580024817.01.T01:CDS 45.0%
CAGTCTTGCGAAAGAGACAA+GGG - Chr5:10173152-10173171 MsG0580024817.01.T01:CDS 45.0%
GCTAGTGTGGAAATGGTTAC+TGG - Chr5:10173291-10173310 MsG0580024817.01.T01:CDS 45.0%
GGGTTGAACAAGGTGAAAAG+TGG - Chr5:10173172-10173191 MsG0580024817.01.T01:CDS 45.0%
GTGTGTGGAAGACGGATATA+TGG - Chr5:10173256-10173275 MsG0580024817.01.T01:CDS 45.0%
TCAGTCTTGCGAAAGAGACA+AGG - Chr5:10173151-10173170 MsG0580024817.01.T01:CDS 45.0%
TTGACGATTCGAATTGGGGT+TGG - Chr5:10173348-10173367 MsG0580024817.01.T01:CDS 45.0%
CACATCCATATCACCGCTTC+TGG + Chr5:10173237-10173256 None:intergenic 50.0%
CGGTGATATGGATGTGTGTG+TGG - Chr5:10173241-10173260 MsG0580024817.01.T01:CDS 50.0%
GGTGAAAAGTGGCGATCAAG+AGG - Chr5:10173183-10173202 MsG0580024817.01.T01:CDS 50.0%
TGGTTACTGGAGAATCCGCA+TGG - Chr5:10173304-10173323 MsG0580024817.01.T01:CDS 50.0%
TGGTTGCGCTAGTGTGGAAA+TGG - Chr5:10173284-10173303 MsG0580024817.01.T01:CDS 50.0%
TGTCACCAGAAGCGGTGATA+TGG - Chr5:10173229-10173248 MsG0580024817.01.T01:CDS 50.0%
TGGCGATCAAGAGGAACTCC+CGG - Chr5:10173192-10173211 MsG0580024817.01.T01:CDS 55.0%
GGCTCTTGGTTGCGCTAGTG+TGG - Chr5:10173278-10173297 MsG0580024817.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 10173102 10173536 10173102 ID=MsG0580024817.01;Name=MsG0580024817.01
Chr5 mRNA 10173102 10173536 10173102 ID=MsG0580024817.01.T01;Parent=MsG0580024817.01;Name=MsG0580024817.01.T01;_AED=0.43;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|144
Chr5 exon 10173102 10173536 10173102 ID=MsG0580024817.01.T01:exon:26030;Parent=MsG0580024817.01.T01
Chr5 CDS 10173102 10173536 10173102 ID=MsG0580024817.01.T01:cds;Parent=MsG0580024817.01.T01
Gene Sequence

>MsG0580024817.01.T01

TTGCCCAACATTCTCGTATTCGAGAATGGAAACATTAAAATTTCAGATTTCAGTCTTGCGAAAGAGACAAGGGTTGAACAAGGTGAAAAGTGGCGATCAAGAGGAACTCCCGGACCAGATAATGATATGTCACCAGAAGCGGTGATATGGATGTGTGTGTGGAAGACGGATATATGGGCTCTTGGTTGCGCTAGTGTGGAAATGGTTACTGGAGAATCCGCATGGAATGTGTCAGATATGTTGATGTTGACGATTCGAATTGGGGTTGGAGAAGAATTACCAAAAATTCCAGATGAATTATTACGAGAAGGGATAGATTTTCTTGAAAAGTGTTTCGTTAAGGATCCTTTGAGAAGATGGACGATAGATCTGCTTTTGAAACATCCGTTTATCTCCGATGTTGAAAATGTTTCAGTTGTAACAGAGTTAAGTTGA

Protein sequence

>MsG0580024817.01.T01

LPNILVFENGNIKISDFSLAKETRVEQGEKWRSRGTPGPDNDMSPEAVIWMCVWKTDIWALGCASVEMVTGESAWNVSDMLMLTIRIGVGEELPKIPDELLREGIDFLEKCFVKDPLRRWTIDLLLKHPFISDVENVSVVTELS*