AlfalfaGEDB | MsG0580024850.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024850.01

KEGG: -
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024850.01.T01	XP_013453506.1	95.046	323	16	0	2	324	3	325	0	568

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024850.01.T01	F4JRB0	39.295	369	155	13	6	328	12	357	9.70E-55	186

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024850.01.T01	A0A072UD38	95.046	323	16	0	2	324	3	325	0.0	568

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TFs/TRs:

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Gene ID	Type	Classification
MsG0580024850.01.T01	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024850.01.T01	MTR_5g017980	95.046	323	16	0	2	324	3	325	0.0	568
MsG0580024850.01.T01	MTR_5g017980	95.046	323	15	1	2	324	3	324	0.0	565
MsG0580024850.01.T01	MTR_5g017980	94.753	324	16	1	2	324	3	326	0.0	564
MsG0580024850.01.T01	MTR_4g086835	39.120	409	149	13	5	323	8	406	7.79e-65	211
MsG0580024850.01.T01	MTR_4g086835	39.024	410	149	14	5	323	8	407	5.49e-63	206
MsG0580024850.01.T01	MTR_4g113140	35.505	307	138	10	9	262	6	305	1.57e-38	142
MsG0580024850.01.T01	MTR_5g054300	36.398	261	101	9	39	252	17	259	5.87e-34	128
MsG0580024850.01.T01	MTR_1g093080	33.333	330	142	15	34	292	2	324	7.32e-34	128
MsG0580024850.01.T01	MTR_1g093080	43.787	169	79	8	94	253	112	273	9.78e-25	101
MsG0580024850.01.T01	MTR_3g106220	39.516	124	64	4	191	305	197	318	1.78e-15	77.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024850.01.T01	AT4G37180	40.055	362	155	12	6	328	12	350	2.15e-58	192
MsG0580024850.01.T01	AT4G37180	39.295	369	155	13	6	328	12	357	9.89e-56	186
MsG0580024850.01.T01	AT4G37180	43.985	266	102	9	6	241	12	260	1.51e-49	167
MsG0580024850.01.T01	AT2G03500	38.408	289	127	11	9	249	6	291	9.90e-41	148
MsG0580024850.01.T01	AT1G68670	35.461	282	117	8	31	255	6	279	1.51e-38	140
MsG0580024850.01.T01	AT1G25550	35.636	275	115	8	31	255	10	272	2.88e-38	139
MsG0580024850.01.T01	AT1G49560	56.154	130	36	5	180	289	171	299	3.86e-33	125
MsG0580024850.01.T01	AT1G25550	34.483	261	110	7	31	241	10	259	6.57e-32	120
MsG0580024850.01.T01	AT1G13300	85.000	60	8	1	191	249	180	239	2.27e-27	110
MsG0580024850.01.T01	AT1G49560	70.833	72	11	2	180	241	171	242	2.51e-24	100

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Find 76 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGGGTTGATGAAAA+TGG	0.274484	5:-10770731	MsG0580024850.01.T01:CDS
AAAATTAGTTACCATCATTA+AGG	0.265474	5:+10770866	None:intergenic
AACCTTTGCAGCAGAGTTCC+AGG	0.347695	5:-10770313	MsG0580024850.01.T01:CDS
AAGAGTGGTGATGGTAGTTC+TGG	0.342533	5:-10770383	MsG0580024850.01.T01:CDS
AAGATGAGCAATCAGATTGC+AGG	0.425444	5:-10769279	MsG0580024850.01.T01:CDS
AAGCCATGGAAGAAACAGTA+TGG	0.440126	5:-10769308	MsG0580024850.01.T01:CDS
AAGTTTCTGAGACATGTCGT+TGG	0.646359	5:+10770978	None:intergenic
AATGGTGGAGGGGTATTTAT+GGG	0.317942	5:-10770485	MsG0580024850.01.T01:CDS
AATTAGGGAAAAGATGCAAG+TGG	0.615575	5:-10769681	MsG0580024850.01.T01:CDS
ACAAAGGAGGTGCTGGTCGT+CGG	0.559002	5:-10770288	MsG0580024850.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAATTTGACCGTACAC+AGG	-	Chr5:10770226-10770245	MsG0580024850.01.T01:intron	30.0%
AACTGTTTGATTAAGATTGG+AGG	-	Chr5:10770184-10770203	MsG0580024850.01.T01:intron	30.0%
AATCAATCTTAGTCGTTGTA+TGG	-	Chr5:10769988-10770007	MsG0580024850.01.T01:intron	30.0%
ATAATCGTATTAACCATGGA+TGG	+	Chr5:10770405-10770424	None:intergenic	30.0%
ATGTGAAGAAGATACATCAA+AGG	-	Chr5:10770583-10770602	MsG0580024850.01.T01:intron	30.0%
ATTAGTTACCATCATTAAGG+AGG	+	Chr5:10769124-10769143	None:intergenic	30.0%
ATTGTGTTACAGAAGAGTAA+TGG	-	Chr5:10769487-10769506	MsG0580024850.01.T01:CDS	30.0%
CCACAATTTCAATTCCAAAT+TGG	+	Chr5:10769900-10769919	None:intergenic	30.0%
GCTCTGATTTATTATGGTAT+AGG	-	Chr5:10769825-10769844	MsG0580024850.01.T01:intron	30.0%
TAATGAAAATCTGTTGAAGG+AGG	-	Chr5:10769531-10769550	MsG0580024850.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	10768899	10771113	10768899	ID=MsG0580024850.01;Name=MsG0580024850.01
Chr5	mRNA	10768899	10771113	10768899	ID=MsG0580024850.01.T01;Parent=MsG0580024850.01;Name=MsG0580024850.01.T01;_AED=0.20;_eAED=0.20;_QI=28\|1\|1\|1\|1\|1\|5\|338\|332
Chr5	exon	10768899	10769512	10768899	ID=MsG0580024850.01.T01:exon:26575;Parent=MsG0580024850.01.T01
Chr5	exon	10769643	10769716	10769643	ID=MsG0580024850.01.T01:exon:26574;Parent=MsG0580024850.01.T01
Chr5	exon	10770237	10770549	10770237	ID=MsG0580024850.01.T01:exon:26573;Parent=MsG0580024850.01.T01
Chr5	exon	10770654	10770781	10770654	ID=MsG0580024850.01.T01:exon:26572;Parent=MsG0580024850.01.T01
Chr5	exon	10770878	10771113	10770878	ID=MsG0580024850.01.T01:exon:26571;Parent=MsG0580024850.01.T01
Chr5	five_prime_UTR	10771086	10771113	10771086	ID=MsG0580024850.01.T01:five_prime_utr;Parent=MsG0580024850.01.T01
Chr5	CDS	10770878	10771085	10770878	ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5	CDS	10770654	10770781	10770654	ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5	CDS	10770237	10770549	10770237	ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5	CDS	10769643	10769716	10769643	ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5	CDS	10769237	10769512	10769237	ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5	three_prime_UTR	10768899	10769236	10768899	ID=MsG0580024850.01.T01:three_prime_utr;Parent=MsG0580024850.01.T01

Gene Sequence

>MsG0580024850.01.T01

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCATTTCCTCAACAACGTTGTTCAAACCAACGACATGTCTCAGAAACTTTCAATGCTTGATGACTTAGTTCATAGCTTGGAAGAAGAAATGAAGAAAGTTCTTGCTTTCAAACGTGAACTCCCTCTTTCCATACTCCTCCTTAATGATGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGAAGATTTGAAAGAAAACTGTGATAATAAGAAGAATTGGATGAGTTCAGCTCAGCTATGGACTAATCAAACAAAAACGAACAATGAAGGTGATGATGATAGGATTGTGTTACAGAAGAGTAATGGTGGAGGGGTATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTTAGTTTGGTGTCTGAAGTGAGTCATGGAAATTCAAAGAGTGGTGATGGTAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCGCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCGGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAGCTTGGTGGAGCACATGCCACTCCTAAGCAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCGTCAATTCAGGAGGCTAATAAGTTAGCCTTGTATATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTTTCAAGCCATGGAAGAAACAGTATGGATGCTGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA

Protein sequence

>MsG0580024850.01.T01

MEQQQLNLDLTLASVPKTVSHFLNNVVQTNDMSQKLSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMEDLKENCDNKKNWMSSAQLWTNQTKTNNEGDDDRIVLQKSNGGGVFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGDGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE*