AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024850.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024850.01.T01 MTR_5g017980 95.046 323 16 0 2 324 3 325 0.0 568
MsG0580024850.01.T01 MTR_5g017980 95.046 323 15 1 2 324 3 324 0.0 565
MsG0580024850.01.T01 MTR_5g017980 94.753 324 16 1 2 324 3 326 0.0 564
MsG0580024850.01.T01 MTR_4g086835 39.120 409 149 13 5 323 8 406 7.79e-65 211
MsG0580024850.01.T01 MTR_4g086835 39.024 410 149 14 5 323 8 407 5.49e-63 206
MsG0580024850.01.T01 MTR_4g113140 35.505 307 138 10 9 262 6 305 1.57e-38 142
MsG0580024850.01.T01 MTR_5g054300 36.398 261 101 9 39 252 17 259 5.87e-34 128
MsG0580024850.01.T01 MTR_1g093080 33.333 330 142 15 34 292 2 324 7.32e-34 128
MsG0580024850.01.T01 MTR_1g093080 43.787 169 79 8 94 253 112 273 9.78e-25 101
MsG0580024850.01.T01 MTR_3g106220 39.516 124 64 4 191 305 197 318 1.78e-15 77.8
MsG0580024850.01.T01 MTR_4g121020 58.333 60 25 0 191 250 214 273 4.86e-15 76.3
MsG0580024850.01.T01 MTR_4g121020 58.333 60 25 0 191 250 214 273 4.91e-15 76.3
MsG0580024850.01.T01 MTR_4g121020 58.333 60 25 0 191 250 110 169 5.53e-15 76.3
MsG0580024850.01.T01 MTR_7g089010 52.542 59 28 0 191 249 90 148 1.49e-14 73.6
MsG0580024850.01.T01 MTR_1g032570 49.315 73 37 0 189 261 128 200 2.25e-14 74.3
MsG0580024850.01.T01 MTR_5g014040 42.697 89 46 2 163 250 285 369 2.27e-14 74.3
MsG0580024850.01.T01 MTR_1g032570 49.315 73 37 0 189 261 197 269 2.41e-14 74.3
MsG0580024850.01.T01 MTR_2g034960 50.704 71 35 0 189 259 208 278 3.09e-14 73.9
MsG0580024850.01.T01 MTR_1g013170 51.562 64 31 0 187 250 186 249 3.78e-14 73.6
MsG0580024850.01.T01 MTR_4g064730 46.032 63 34 0 188 250 127 189 2.33e-13 70.1
MsG0580024850.01.T01 MTR_2g450070 49.180 61 31 0 189 249 210 270 2.43e-13 71.2
MsG0580024850.01.T01 MTR_2g450070 49.180 61 31 0 189 249 210 270 2.43e-13 71.2
MsG0580024850.01.T01 MTR_2g027860 55.714 70 29 2 192 260 49 117 3.97e-13 69.7
MsG0580024850.01.T01 MTR_5g041350 60.000 55 21 1 192 245 15 69 1.22e-12 68.2
MsG0580024850.01.T01 MTR_4g098870 44.615 65 36 0 186 250 304 368 1.57e-12 68.6
MsG0580024850.01.T01 MTR_7g117705 46.377 69 37 0 191 259 190 258 1.58e-12 68.6
MsG0580024850.01.T01 MTR_4g098870 44.615 65 36 0 186 250 324 388 1.65e-12 68.6
MsG0580024850.01.T01 MTR_4g098870 44.615 65 36 0 186 250 343 407 1.69e-12 68.6
MsG0580024850.01.T01 MTR_4g098870 44.615 65 36 0 186 250 304 368 2.03e-12 68.2
MsG0580024850.01.T01 MTR_4g098870 44.615 65 36 0 186 250 324 388 2.15e-12 68.2
MsG0580024850.01.T01 MTR_5g014040 40.426 94 46 3 163 250 285 374 2.34e-12 68.2
MsG0580024850.01.T01 MTR_4g131580 50.847 59 29 0 191 249 199 257 2.53e-12 68.2
MsG0580024850.01.T01 MTR_5g014040 40.426 94 46 3 163 250 245 334 2.55e-12 67.8
MsG0580024850.01.T01 MTR_1g090670 58.621 58 23 1 189 245 41 98 3.36e-12 66.6
MsG0580024850.01.T01 MTR_4g078810 52.542 59 28 0 188 246 154 212 5.52e-12 66.6
MsG0580024850.01.T01 MTR_2g027800 60.000 55 21 1 192 245 39 93 1.07e-11 65.1
MsG0580024850.01.T01 MTR_4g021790 47.619 63 33 0 187 249 163 225 1.22e-11 64.7
MsG0580024850.01.T01 MTR_1g053830 50.877 57 27 1 190 245 16 72 1.26e-11 60.8
MsG0580024850.01.T01 MTR_3g102600 48.148 54 28 0 196 249 87 140 1.29e-11 62.8
MsG0580024850.01.T01 MTR_2g027800 60.000 55 21 1 192 245 39 93 1.34e-11 65.1
MsG0580024850.01.T01 MTR_4g131570 46.032 63 34 0 187 249 198 260 1.36e-11 65.9
MsG0580024850.01.T01 MTR_4g131600 46.552 58 31 0 191 248 200 257 1.46e-11 65.9
MsG0580024850.01.T01 MTR_1g080330 53.731 67 29 2 189 254 172 237 1.69e-11 65.1
MsG0580024850.01.T01 MTR_1g080330 53.731 67 29 2 189 254 172 237 1.70e-11 64.3
MsG0580024850.01.T01 MTR_3g102600 48.148 54 28 0 196 249 136 189 2.66e-11 63.2
MsG0580024850.01.T01 MTR_2g090675 53.571 56 26 0 191 246 160 215 2.92e-11 64.3
MsG0580024850.01.T01 MTR_7g098250 52.239 67 30 2 189 254 259 324 3.09e-11 64.7
MsG0580024850.01.T01 MTR_7g115530 59.259 54 21 1 192 244 218 271 4.42e-11 63.2
MsG0580024850.01.T01 MTR_1g013180 47.458 59 31 0 191 249 341 399 4.49e-11 64.3
MsG0580024850.01.T01 MTR_7g115530 59.259 54 21 1 192 244 218 271 7.14e-11 62.8
MsG0580024850.01.T01 MTR_7g115530 59.259 54 21 1 192 244 218 271 7.84e-11 62.8
MsG0580024850.01.T01 MTR_3g086100 46.774 62 33 0 189 250 125 186 8.02e-11 63.2
MsG0580024850.01.T01 MTR_2g086450 38.679 106 49 3 141 245 9 99 9.35e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024850.01.T01 AT4G37180 40.055 362 155 12 6 328 12 350 2.15e-58 192
MsG0580024850.01.T01 AT4G37180 39.295 369 155 13 6 328 12 357 9.89e-56 186
MsG0580024850.01.T01 AT4G37180 43.985 266 102 9 6 241 12 260 1.51e-49 167
MsG0580024850.01.T01 AT2G03500 38.408 289 127 11 9 249 6 291 9.90e-41 148
MsG0580024850.01.T01 AT1G68670 35.461 282 117 8 31 255 6 279 1.51e-38 140
MsG0580024850.01.T01 AT1G25550 35.636 275 115 8 31 255 10 272 2.88e-38 139
MsG0580024850.01.T01 AT1G49560 56.154 130 36 5 180 289 171 299 3.86e-33 125
MsG0580024850.01.T01 AT1G25550 34.483 261 110 7 31 241 10 259 6.57e-32 120
MsG0580024850.01.T01 AT1G13300 85.000 60 8 1 191 249 180 239 2.27e-27 110
MsG0580024850.01.T01 AT1G49560 70.833 72 11 2 180 241 171 242 2.51e-24 100
MsG0580024850.01.T01 AT3G25790 81.818 55 9 1 196 249 200 254 9.96e-24 100
MsG0580024850.01.T01 AT3G25790 80.851 47 8 1 196 241 200 246 1.29e-18 84.3
MsG0580024850.01.T01 AT5G05090 52.542 59 28 0 191 249 80 138 5.21e-15 74.3
MsG0580024850.01.T01 AT3G10760 52.542 59 28 0 191 249 104 162 8.98e-15 74.7
MsG0580024850.01.T01 AT5G59570 47.619 63 33 0 188 250 137 199 1.47e-14 73.6
MsG0580024850.01.T01 AT5G59570 47.619 63 33 0 188 250 137 199 1.47e-14 73.6
MsG0580024850.01.T01 AT2G40970 52.542 59 28 0 191 249 104 162 2.00e-14 72.4
MsG0580024850.01.T01 AT3G16857 55.000 60 27 0 191 250 166 225 9.45e-14 72.4
MsG0580024850.01.T01 AT3G16857 55.000 60 27 0 191 250 236 295 1.14e-13 72.4
MsG0580024850.01.T01 AT5G58080 50.000 62 31 0 189 250 191 252 1.15e-13 72.4
MsG0580024850.01.T01 AT3G16857 55.000 60 27 0 191 250 236 295 1.27e-13 72.0
MsG0580024850.01.T01 AT3G46640 48.333 60 31 0 191 250 143 202 2.34e-13 70.1
MsG0580024850.01.T01 AT3G46640 48.333 60 31 0 191 250 143 202 2.34e-13 70.1
MsG0580024850.01.T01 AT3G46640 48.333 60 31 0 191 250 143 202 2.80e-13 70.1
MsG0580024850.01.T01 AT2G01060 54.930 71 30 2 185 254 8 77 3.86e-13 69.3
MsG0580024850.01.T01 AT2G20400 44.828 87 46 2 192 277 231 316 7.04e-13 69.3
MsG0580024850.01.T01 AT2G25180 47.059 68 36 0 191 258 194 261 1.07e-12 69.3
MsG0580024850.01.T01 AT2G01760 50.820 61 30 0 190 250 226 286 1.13e-12 68.9
MsG0580024850.01.T01 AT2G01760 50.820 61 30 0 190 250 133 193 1.15e-12 68.2
MsG0580024850.01.T01 AT2G01760 50.820 61 30 0 190 250 198 258 1.20e-12 68.6
MsG0580024850.01.T01 AT4G31920 41.333 75 44 0 189 263 180 254 1.64e-12 68.6
MsG0580024850.01.T01 AT2G20400 46.512 86 42 3 192 276 231 313 2.80e-12 67.4
MsG0580024850.01.T01 AT2G06020 50.000 64 31 1 183 245 77 140 3.66e-12 66.6
MsG0580024850.01.T01 AT3G24120 60.000 55 21 1 192 245 41 95 6.89e-12 65.9
MsG0580024850.01.T01 AT3G24120 60.000 55 21 1 192 245 41 95 6.92e-12 65.5
MsG0580024850.01.T01 AT3G04030 55.556 54 24 0 192 245 45 98 7.10e-12 66.2
MsG0580024850.01.T01 AT3G04030 56.364 55 23 1 192 245 45 99 4.98e-11 63.5
MsG0580024850.01.T01 AT3G04030 56.364 55 23 1 192 245 45 99 5.50e-11 63.5
MsG0580024850.01.T01 AT2G20570 45.833 72 39 0 188 259 149 220 5.57e-11 63.5
MsG0580024850.01.T01 AT5G06800 54.545 55 24 1 192 245 192 246 6.29e-11 62.8

Find 76 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGGTGGAGGGGTATTTA+TGG 0.172244 5:-10770486 MsG0580024850.01.T01:CDS
CAGCCACTCCTAAGCAAATT+AGG 0.199099 5:-10769697 MsG0580024850.01.T01:CDS
AAAATTAGTTACCATCATTA+AGG 0.265474 5:+10770866 None:intergenic
CCAGCACCTCCTTTGTTTCC+TGG 0.269810 5:+10770295 None:intergenic
AAAAGTTGGGTTGATGAAAA+TGG 0.274484 5:-10770731 MsG0580024850.01.T01:CDS
ATTCCCAGTTTCGTCAATTC+AGG 0.284606 5:-10769467 MsG0580024850.01.T01:CDS
CAGCACTCAGCAAAATTTAG+AGG 0.286569 5:+10769204 None:intergenic
AGCAATCAGATTGCAGGAAT+TGG 0.302511 5:-10769273 MsG0580024850.01.T01:CDS
CTCCTTGGTGGATCTGTTCA+AGG 0.312173 5:-10769337 MsG0580024850.01.T01:CDS
GCCTCCTGAATTGACGAAAC+TGG 0.313674 5:+10769463 None:intergenic
AATGGTGGAGGGGTATTTAT+GGG 0.317942 5:-10770485 MsG0580024850.01.T01:CDS
AAGAGTGGTGATGGTAGTTC+TGG 0.342533 5:-10770383 MsG0580024850.01.T01:CDS
AACCTTTGCAGCAGAGTTCC+AGG 0.347695 5:-10770313 MsG0580024850.01.T01:CDS
ACAAGCTAAGAGCTTTAGTT+TGG 0.377820 5:-10770432 MsG0580024850.01.T01:CDS
ACTGTGATAATAAGAAGAAT+TGG 0.383175 5:-10770693 MsG0580024850.01.T01:CDS
AGCCACTCCTAAGCAAATTA+GGG 0.392450 5:-10769696 MsG0580024850.01.T01:CDS
CTCAGAGTCTGTTTCTCCAC+AGG 0.393005 5:-10769377 MsG0580024850.01.T01:CDS
TGCAAAGGTTGCGGCGGCTG+AGG 0.403383 5:+10770326 None:intergenic
CATCCATACTGTTTCTTCCA+TGG 0.409764 5:+10769305 None:intergenic
TTGTGATTATGCAGAAGTAT+AGG 0.413901 5:-10769504 MsG0580024850.01.T01:intron
CCTCTTACTCCTCTTCTCCT+TGG 0.417068 5:-10769352 MsG0580024850.01.T01:CDS
TCTTTCATATCTGATATTGA+TGG 0.420948 5:-10768995 MsG0580024850.01.T01:three_prime_UTR
AAGATGAGCAATCAGATTGC+AGG 0.425444 5:-10769279 MsG0580024850.01.T01:CDS
TAAGGAGGAGTATGGAAAGA+GGG 0.427844 5:+10770884 None:intergenic
TAATAAGTTAGCCTTGTATA+TGG 0.434530 5:-10769440 MsG0580024850.01.T01:CDS
ACCATCATTAAGGAGGAGTA+TGG 0.434982 5:+10770876 None:intergenic
AAGCCATGGAAGAAACAGTA+TGG 0.440126 5:-10769308 MsG0580024850.01.T01:CDS
CTTACTCCTCTTCTCCTTGG+TGG 0.445651 5:-10769349 MsG0580024850.01.T01:CDS
ACAATGAAGGTGATGATGAT+AGG 0.445952 5:-10770526 MsG0580024850.01.T01:CDS
TATTGTCACTCACTTGCAAA+TGG 0.459590 5:+10769630 None:intergenic
TCCATACTCCTCCTTAATGA+TGG 0.469852 5:-10770877 MsG0580024850.01.T01:intron
CCAAGGAGAAGAGGAGTAAG+AGG 0.473664 5:+10769352 None:intergenic
TTCCCTAATTTGCTTAGGAG+TGG 0.487933 5:+10769694 None:intergenic
TTAAGGAGGAGTATGGAAAG+AGG 0.491286 5:+10770883 None:intergenic
CAGGAATTGGAAAAGTGATC+AGG 0.500799 5:-10769260 MsG0580024850.01.T01:CDS
ATTTAATGAAAATCTGTTGA+AGG 0.503957 5:-10770462 MsG0580024850.01.T01:CDS
TTCCTGGAACTCTGCTGCAA+AGG 0.504800 5:+10770311 None:intergenic
GTTGAAGTGCATCAACAAAT+CGG 0.508453 5:+10770255 None:intergenic
TGATGACTTAGTTCATAGCT+TGG 0.508826 5:-10770947 MsG0580024850.01.T01:CDS
CCTCCTGAATTGACGAAACT+GGG 0.520520 5:+10769464 None:intergenic
ACAGTTACATGCAACTGTAC+TGG 0.524248 5:+10768957 None:intergenic
CCAGGAAACAAAGGAGGTGC+TGG 0.526510 5:-10770295 MsG0580024850.01.T01:CDS
GTTCAAGGTCTTTCAAGCCA+TGG 0.531430 5:-10769322 MsG0580024850.01.T01:CDS
GAACTCTGCTGCAAAGGTTG+CGG 0.536482 5:+10770317 None:intergenic
AGGGAAAAGATGCAAGTGGA+TGG 0.538779 5:-10769677 MsG0580024850.01.T01:CDS
TCAGAGTCTGTTTCTCCACA+GGG 0.539092 5:-10769376 MsG0580024850.01.T01:CDS
GGATGAGTTCAGCTCAGCTA+TGG 0.541177 5:-10770672 MsG0580024850.01.T01:CDS
GGAAATTCAAAGAGTGGTGA+TGG 0.543852 5:-10770392 MsG0580024850.01.T01:CDS
GTTGTTGAGGAAATGAGAAA+CGG 0.545414 5:+10771011 None:intergenic
TAATGAAAATCTGTTGAAGG+AGG 0.547757 5:-10770459 MsG0580024850.01.T01:CDS
CTCTGCTGCAAAGGTTGCGG+CGG 0.547761 5:+10770320 None:intergenic
TGGTTTGAACAACGTTGTTG+AGG 0.552246 5:+10770998 None:intergenic
TGTGAAGAAGATACATCAAA+GGG 0.552624 5:-10769406 MsG0580024850.01.T01:CDS
TTCATATCTGATATTGATGG+AGG 0.558322 5:-10768992 MsG0580024850.01.T01:three_prime_UTR
CCCAGTTTCGTCAATTCAGG+AGG 0.558843 5:-10769464 MsG0580024850.01.T01:CDS
ACAAAGGAGGTGCTGGTCGT+CGG 0.559002 5:-10770288 MsG0580024850.01.T01:CDS
CAACAGCTTGGTGGAGCACA+TGG 0.565013 5:-10770236 MsG0580024850.01.T01:intron
GTTGATGCACTTCAACAGCT+TGG 0.565906 5:-10770248 MsG0580024850.01.T01:CDS
ACAGAAGAGTAATGGTGGAG+GGG 0.566775 5:-10770495 MsG0580024850.01.T01:CDS
ACAGATCCACCAAGGAGAAG+AGG 0.569987 5:+10769343 None:intergenic
GTGTTACAGAAGAGTAATGG+TGG 0.573520 5:-10770500 MsG0580024850.01.T01:CDS
ATGTGAAGAAGATACATCAA+AGG 0.582691 5:-10769407 MsG0580024850.01.T01:CDS
GATGCACTTCAACAGCTTGG+TGG 0.583631 5:-10770245 MsG0580024850.01.T01:CDS
ATTAGTTACCATCATTAAGG+AGG 0.585189 5:+10770869 None:intergenic
AGCAGAGTTCCAGGAAACAA+AGG 0.588997 5:-10770304 MsG0580024850.01.T01:CDS
ATTGTGTTACAGAAGAGTAA+TGG 0.592672 5:-10770503 MsG0580024850.01.T01:CDS
TTACAGAAGAGTAATGGTGG+AGG 0.599891 5:-10770497 MsG0580024850.01.T01:CDS
TACAGAAGAGTAATGGTGGA+GGG 0.606656 5:-10770496 MsG0580024850.01.T01:CDS
AATTAGGGAAAAGATGCAAG+TGG 0.615575 5:-10769681 MsG0580024850.01.T01:CDS
TTGATCTTGAGCCATATACA+AGG 0.616538 5:+10769429 None:intergenic
AGTCATGGAAATTCAAAGAG+TGG 0.617489 5:-10770398 MsG0580024850.01.T01:CDS
TTGGTGTCTGAAGTGAGTCA+TGG 0.626831 5:-10770413 MsG0580024850.01.T01:CDS
AAGTTTCTGAGACATGTCGT+TGG 0.646359 5:+10770978 None:intergenic
AGAGGAGTAAGAGGACCCTG+TGG 0.650364 5:+10769361 None:intergenic
AGAGTTCCAGGAAACAAAGG+AGG 0.672803 5:-10770301 MsG0580024850.01.T01:CDS
GACCTTGAACAGATCCACCA+AGG 0.687479 5:+10769335 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATCACAATTAAAAAAA+AGG + Chr5:10769396-10769415 None:intergenic 10.0%
!! TAAAAATTAACAAAAAGAAA+AGG + Chr5:10769190-10769209 None:intergenic 10.0%
!!! AATTTTCTAATTAATTTTTG+TGG - Chr5:10769141-10769160 MsG0580024850.01.T01:three_prime_UTR 10.0%
!!! GATAAAAAAAATGTTTTTTT+TGG - Chr5:10769437-10769456 MsG0580024850.01.T01:CDS 10.0%
!! AAAAAGAAAAAACATCATCA+TGG - Chr5:10768908-10768927 MsG0580024850.01.T01:three_prime_UTR 20.0%
!! AAAATTAGTTACCATCATTA+AGG + Chr5:10769127-10769146 None:intergenic 20.0%
!! AATGATGAAAAAGAAAAAGT+TGG - Chr5:10769245-10769264 MsG0580024850.01.T01:CDS 20.0%
!! ATGATGAAAAAGAAAAAGTT+GGG - Chr5:10769246-10769265 MsG0580024850.01.T01:CDS 20.0%
!! ATTTAATGAAAATCTGTTGA+AGG - Chr5:10769528-10769547 MsG0580024850.01.T01:intron 20.0%
!! TACAAAAAAAAAAACCAGTT+AGG + Chr5:10770272-10770291 None:intergenic 20.0%
!! TATGAAAAACAAAATCACTA+TGG + Chr5:10770917-10770936 None:intergenic 20.0%
!!! AGTTCTAAGTTATAACAAAT+AGG + Chr5:10770168-10770187 None:intergenic 20.0%
!!! TGTTTTTCATAATTATGCTA+TGG - Chr5:10770926-10770945 MsG0580024850.01.T01:CDS 20.0%
! ACTAATAATCGTATTAACCA+TGG + Chr5:10770409-10770428 None:intergenic 25.0%
! ACTGTGATAATAAGAAGAAT+TGG - Chr5:10769297-10769316 MsG0580024850.01.T01:CDS 25.0%
! AGATTACATGACATGTTATT+AGG - Chr5:10770128-10770147 MsG0580024850.01.T01:intron 25.0%
! GATTACATGACATGTTATTA+GGG - Chr5:10770129-10770148 MsG0580024850.01.T01:intron 25.0%
! TAGAACTGTTTGATTAAGAT+TGG - Chr5:10770181-10770200 MsG0580024850.01.T01:intron 25.0%
! TCTTTCATATCTGATATTGA+TGG - Chr5:10770995-10771014 MsG0580024850.01.T01:CDS 25.0%
!! ATGGACTAATCAAACAAAAA+CGG - Chr5:10769337-10769356 MsG0580024850.01.T01:CDS 25.0%
!! ATGTTATTAGGGATTAGTAT+AGG - Chr5:10770140-10770159 MsG0580024850.01.T01:intron 25.0%
!! CAATTTGGAATTGAAATTGT+GGG - Chr5:10769898-10769917 MsG0580024850.01.T01:intron 25.0%
!! TCTTAGTGTCTATTTTGTAT+TGG - Chr5:10769852-10769871 MsG0580024850.01.T01:intron 25.0%
!! TGTCTATTTTGTATTGGATT+TGG - Chr5:10769858-10769877 MsG0580024850.01.T01:intron 25.0%
!!! TAATAAGTTAGCCTTGTATA+TGG - Chr5:10770550-10770569 MsG0580024850.01.T01:intron 25.0%
!!! TTGTGGTATTTCTTGTTTTT+TGG - Chr5:10769158-10769177 MsG0580024850.01.T01:three_prime_UTR 25.0%
!!! TTTTTTTGGTAGAACAATGA+AGG - Chr5:10769451-10769470 MsG0580024850.01.T01:CDS 25.0%
AAAAAAATTTGACCGTACAC+AGG - Chr5:10770226-10770245 MsG0580024850.01.T01:intron 30.0%
AACTGTTTGATTAAGATTGG+AGG - Chr5:10770184-10770203 MsG0580024850.01.T01:intron 30.0%
AATCAATCTTAGTCGTTGTA+TGG - Chr5:10769988-10770007 MsG0580024850.01.T01:intron 30.0%
ATAATCGTATTAACCATGGA+TGG + Chr5:10770405-10770424 None:intergenic 30.0%
ATGTGAAGAAGATACATCAA+AGG - Chr5:10770583-10770602 MsG0580024850.01.T01:intron 30.0%
ATTAGTTACCATCATTAAGG+AGG + Chr5:10769124-10769143 None:intergenic 30.0%
ATTGTGTTACAGAAGAGTAA+TGG - Chr5:10769487-10769506 MsG0580024850.01.T01:CDS 30.0%
CCACAATTTCAATTCCAAAT+TGG + Chr5:10769900-10769919 None:intergenic 30.0%
GCTCTGATTTATTATGGTAT+AGG - Chr5:10769825-10769844 MsG0580024850.01.T01:intron 30.0%
TAATGAAAATCTGTTGAAGG+AGG - Chr5:10769531-10769550 MsG0580024850.01.T01:intron 30.0%
TGTGAAGAAGATACATCAAA+GGG - Chr5:10770584-10770603 MsG0580024850.01.T01:intron 30.0%
TTCATATCTGATATTGATGG+AGG - Chr5:10770998-10771017 MsG0580024850.01.T01:CDS 30.0%
! AGTGTTGCTCTGATTTATTA+TGG - Chr5:10769819-10769838 MsG0580024850.01.T01:intron 30.0%
! ATAGAGTTGATTCTCCAATT+TGG - Chr5:10769883-10769902 MsG0580024850.01.T01:intron 30.0%
! CCAATTTGGAATTGAAATTG+TGG - Chr5:10769897-10769916 MsG0580024850.01.T01:intron 30.0%
! TTGTGATTATGCAGAAGTAT+AGG - Chr5:10770486-10770505 MsG0580024850.01.T01:CDS 30.0%
!! AAAAGTTGGGTTGATGAAAA+TGG - Chr5:10769259-10769278 MsG0580024850.01.T01:CDS 30.0%
!!! ATCTTTTCCCTAATTTGCTT+AGG + Chr5:10770304-10770323 None:intergenic 30.0%
!!! ATGGTTATTTTAGACTGTTG+TGG - Chr5:10770945-10770964 MsG0580024850.01.T01:CDS 30.0%
!!! TTTTTTTGCAATCAGCAAGA+TGG + Chr5:10770213-10770232 None:intergenic 30.0%
AATTAGGGAAAAGATGCAAG+TGG - Chr5:10770309-10770328 MsG0580024850.01.T01:CDS 35.0%
AGTCATGGAAATTCAAAGAG+TGG - Chr5:10769592-10769611 MsG0580024850.01.T01:intron 35.0%
GTTGAAGTGCATCAACAAAT+CGG + Chr5:10769738-10769757 None:intergenic 35.0%
GTTGTTGAGGAAATGAGAAA+CGG + Chr5:10768982-10769001 None:intergenic 35.0%
TATTGTCACTCACTTGCAAA+TGG + Chr5:10770363-10770382 None:intergenic 35.0%
TGATGACTTAGTTCATAGCT+TGG - Chr5:10769043-10769062 MsG0580024850.01.T01:three_prime_UTR 35.0%
TTGATCTTGAGCCATATACA+AGG + Chr5:10770564-10770583 None:intergenic 35.0%
! ACAAGCTAAGAGCTTTAGTT+TGG - Chr5:10769558-10769577 MsG0580024850.01.T01:intron 35.0%
! ACAATGAAGGTGATGATGAT+AGG - Chr5:10769464-10769483 MsG0580024850.01.T01:CDS 35.0%
! AGGAAATGAGAAACGGTTTT+TGG + Chr5:10768975-10768994 None:intergenic 35.0%
! TGAAATTGTGGGTCTTGTAT+GGG - Chr5:10769909-10769928 MsG0580024850.01.T01:intron 35.0%
! TTGAAATTGTGGGTCTTGTA+TGG - Chr5:10769908-10769927 MsG0580024850.01.T01:intron 35.0%
AAGATGAGCAATCAGATTGC+AGG - Chr5:10770711-10770730 MsG0580024850.01.T01:CDS 40.0%
AAGCCATGGAAGAAACAGTA+TGG - Chr5:10770682-10770701 MsG0580024850.01.T01:CDS 40.0%
AATCTGGATTACCTGCAGTT+AGG - Chr5:10769942-10769961 MsG0580024850.01.T01:intron 40.0%
ACAGTTACATGCAACTGTAC+TGG + Chr5:10771036-10771055 None:intergenic 40.0%
ACCATCATTAAGGAGGAGTA+TGG + Chr5:10769117-10769136 None:intergenic 40.0%
ACCTGCAGTTAGGAAATATG+TGG - Chr5:10769952-10769971 MsG0580024850.01.T01:intron 40.0%
AGCAATCAGATTGCAGGAAT+TGG - Chr5:10770717-10770736 MsG0580024850.01.T01:CDS 40.0%
AGCCACTCCTAAGCAAATTA+GGG - Chr5:10770294-10770313 MsG0580024850.01.T01:CDS 40.0%
ATTCCCAGTTTCGTCAATTC+AGG - Chr5:10770523-10770542 MsG0580024850.01.T01:CDS 40.0%
CAGCACTCAGCAAAATTTAG+AGG + Chr5:10770789-10770808 None:intergenic 40.0%
CAGGAATTGGAAAAGTGATC+AGG - Chr5:10770730-10770749 MsG0580024850.01.T01:CDS 40.0%
CGTATTAACCATGGATGGTT+TGG + Chr5:10770400-10770419 None:intergenic 40.0%
GAATCCCACTCTTGTGTATA+GGG - Chr5:10770101-10770120 MsG0580024850.01.T01:intron 40.0%
GGAAATTCAAAGAGTGGTGA+TGG - Chr5:10769598-10769617 MsG0580024850.01.T01:intron 40.0%
GTGTTACAGAAGAGTAATGG+TGG - Chr5:10769490-10769509 MsG0580024850.01.T01:CDS 40.0%
GTTGTAAGATGCAGATCAGA+TGG + Chr5:10770064-10770083 None:intergenic 40.0%
TCCACATATTTCCTAACTGC+AGG + Chr5:10769956-10769975 None:intergenic 40.0%
TCCATACTCCTCCTTAATGA+TGG - Chr5:10769113-10769132 MsG0580024850.01.T01:three_prime_UTR 40.0%
TGATCCCTATACACAAGAGT+GGG + Chr5:10770108-10770127 None:intergenic 40.0%
TGGTTTGAACAACGTTGTTG+AGG + Chr5:10768995-10769014 None:intergenic 40.0%
TTACAGAAGAGTAATGGTGG+AGG - Chr5:10769493-10769512 MsG0580024850.01.T01:CDS 40.0%
TTGATCCCTATACACAAGAG+TGG + Chr5:10770109-10770128 None:intergenic 40.0%
! AAATTGTGGGTCTTGTATGG+GGG - Chr5:10769911-10769930 MsG0580024850.01.T01:intron 40.0%
! CATCCATACTGTTTCTTCCA+TGG + Chr5:10770688-10770707 None:intergenic 40.0%
! GAAATTGTGGGTCTTGTATG+GGG - Chr5:10769910-10769929 MsG0580024850.01.T01:intron 40.0%
! TAAGGAGGAGTATGGAAAGA+GGG + Chr5:10769109-10769128 None:intergenic 40.0%
! TACAGAAGAGTAATGGTGGA+GGG - Chr5:10769494-10769513 MsG0580024850.01.T01:intron 40.0%
! TTAAGGAGGAGTATGGAAAG+AGG + Chr5:10769110-10769129 None:intergenic 40.0%
!! AAGTTTCTGAGACATGTCGT+TGG + Chr5:10769015-10769034 None:intergenic 40.0%
!! AATGGTGGAGGGGTATTTAT+GGG - Chr5:10769505-10769524 MsG0580024850.01.T01:intron 40.0%
!! TAATGGTGGAGGGGTATTTA+TGG - Chr5:10769504-10769523 MsG0580024850.01.T01:intron 40.0%
!! TTCCCTAATTTGCTTAGGAG+TGG + Chr5:10770299-10770318 None:intergenic 40.0%
!!! AGAAACGGTTTTTGGAACAG+AGG + Chr5:10768967-10768986 None:intergenic 40.0%
AAGATGCAGATCAGATGGCT+TGG + Chr5:10770059-10770078 None:intergenic 45.0%
ACAACTGAGACAACCACTAC+AGG - Chr5:10770079-10770098 MsG0580024850.01.T01:intron 45.0%
AGAGTTCCAGGAAACAAAGG+AGG - Chr5:10769689-10769708 MsG0580024850.01.T01:CDS 45.0%
AGCAGAGTTCCAGGAAACAA+AGG - Chr5:10769686-10769705 MsG0580024850.01.T01:CDS 45.0%
AGGGAAAAGATGCAAGTGGA+TGG - Chr5:10770313-10770332 MsG0580024850.01.T01:CDS 45.0%
CAGCCACTCCTAAGCAAATT+AGG - Chr5:10770293-10770312 MsG0580024850.01.T01:CDS 45.0%
CCTCCTGAATTGACGAAACT+GGG + Chr5:10770529-10770548 None:intergenic 45.0%
CTCAAACTCCAAACCATCCA+TGG - Chr5:10770389-10770408 MsG0580024850.01.T01:CDS 45.0%
GGAATCCCACTCTTGTGTAT+AGG - Chr5:10770100-10770119 MsG0580024850.01.T01:intron 45.0%
GTTCAAGGTCTTTCAAGCCA+TGG - Chr5:10770668-10770687 MsG0580024850.01.T01:CDS 45.0%
GTTGATGCACTTCAACAGCT+TGG - Chr5:10769742-10769761 MsG0580024850.01.T01:intron 45.0%
! AATTGTGGGTCTTGTATGGG+GGG - Chr5:10769912-10769931 MsG0580024850.01.T01:intron 45.0%
! ACAGAAGAGTAATGGTGGAG+GGG - Chr5:10769495-10769514 MsG0580024850.01.T01:intron 45.0%
! TCAGAGTCTGTTTCTCCACA+GGG - Chr5:10770614-10770633 MsG0580024850.01.T01:intron 45.0%
! TTGGTGTCTGAAGTGAGTCA+TGG - Chr5:10769577-10769596 MsG0580024850.01.T01:intron 45.0%
!! AAGAGTGGTGATGGTAGTTC+TGG - Chr5:10769607-10769626 MsG0580024850.01.T01:intron 45.0%
AACCTTTGCAGCAGAGTTCC+AGG - Chr5:10769677-10769696 MsG0580024850.01.T01:CDS 50.0%
ACAGATCCACCAAGGAGAAG+AGG + Chr5:10770650-10770669 None:intergenic 50.0%
AGGGTGTGTATGAGCCTAAC+TGG - Chr5:10770255-10770274 MsG0580024850.01.T01:CDS 50.0%
CAAGAGTGGGATTCCTGTAG+TGG + Chr5:10770095-10770114 None:intergenic 50.0%
CACCCTACATAGCCTGTGTA+CGG + Chr5:10770241-10770260 None:intergenic 50.0%
CCAAGGAGAAGAGGAGTAAG+AGG + Chr5:10770641-10770660 None:intergenic 50.0%
CCCAGTTTCGTCAATTCAGG+AGG - Chr5:10770526-10770545 MsG0580024850.01.T01:CDS 50.0%
CCTCTTACTCCTCTTCTCCT+TGG - Chr5:10770638-10770657 MsG0580024850.01.T01:intron 50.0%
CTTACTCCTCTTCTCCTTGG+TGG - Chr5:10770641-10770660 MsG0580024850.01.T01:intron 50.0%
GAACTCTGCTGCAAAGGTTG+CGG + Chr5:10769676-10769695 None:intergenic 50.0%
GACCGTACACAGGCTATGTA+GGG - Chr5:10770236-10770255 MsG0580024850.01.T01:intron 50.0%
GACCTTGAACAGATCCACCA+AGG + Chr5:10770658-10770677 None:intergenic 50.0%
GATGCACTTCAACAGCTTGG+TGG - Chr5:10769745-10769764 MsG0580024850.01.T01:intron 50.0%
GCCTCCTGAATTGACGAAAC+TGG + Chr5:10770530-10770549 None:intergenic 50.0%
GGATGAGTTCAGCTCAGCTA+TGG - Chr5:10769318-10769337 MsG0580024850.01.T01:CDS 50.0%
TGACCGTACACAGGCTATGT+AGG - Chr5:10770235-10770254 MsG0580024850.01.T01:intron 50.0%
TTCCTGGAACTCTGCTGCAA+AGG + Chr5:10769682-10769701 None:intergenic 50.0%
! ATTGTGGGTCTTGTATGGGG+GGG - Chr5:10769913-10769932 MsG0580024850.01.T01:intron 50.0%
! CTCAGAGTCTGTTTCTCCAC+AGG - Chr5:10770613-10770632 MsG0580024850.01.T01:intron 50.0%
! CTCCTTGGTGGATCTGTTCA+AGG - Chr5:10770653-10770672 MsG0580024850.01.T01:intron 50.0%
AGAGGAGTAAGAGGACCCTG+TGG + Chr5:10770632-10770651 None:intergenic 55.0%
! CAACAGCTTGGTGGAGCACA+TGG - Chr5:10769754-10769773 MsG0580024850.01.T01:intron 55.0%
! CCAGCACCTCCTTTGTTTCC+TGG + Chr5:10769698-10769717 None:intergenic 55.0%
! CCAGGAAACAAAGGAGGTGC+TGG - Chr5:10769695-10769714 MsG0580024850.01.T01:CDS 55.0%
! TTGTGGGTCTTGTATGGGGG+GGG - Chr5:10769914-10769933 MsG0580024850.01.T01:intron 55.0%
!! ACAAAGGAGGTGCTGGTCGT+CGG - Chr5:10769702-10769721 MsG0580024850.01.T01:intron 55.0%
CTCTGCTGCAAAGGTTGCGG+CGG + Chr5:10769673-10769692 None:intergenic 60.0%
! TGTGGGTCTTGTATGGGGGG+GGG - Chr5:10769915-10769934 MsG0580024850.01.T01:intron 60.0%
TATGGGGGGGGGGAGCAATC+TGG - Chr5:10769926-10769945 MsG0580024850.01.T01:intron 65.0%
TGCAAAGGTTGCGGCGGCTG+AGG + Chr5:10769667-10769686 None:intergenic 65.0%
! GTGGGTCTTGTATGGGGGGG+GGG - Chr5:10769916-10769935 MsG0580024850.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 10768899 10771113 10768899 ID=MsG0580024850.01;Name=MsG0580024850.01
Chr5 mRNA 10768899 10771113 10768899 ID=MsG0580024850.01.T01;Parent=MsG0580024850.01;Name=MsG0580024850.01.T01;_AED=0.20;_eAED=0.20;_QI=28|1|1|1|1|1|5|338|332
Chr5 exon 10768899 10769512 10768899 ID=MsG0580024850.01.T01:exon:26575;Parent=MsG0580024850.01.T01
Chr5 exon 10769643 10769716 10769643 ID=MsG0580024850.01.T01:exon:26574;Parent=MsG0580024850.01.T01
Chr5 exon 10770237 10770549 10770237 ID=MsG0580024850.01.T01:exon:26573;Parent=MsG0580024850.01.T01
Chr5 exon 10770654 10770781 10770654 ID=MsG0580024850.01.T01:exon:26572;Parent=MsG0580024850.01.T01
Chr5 exon 10770878 10771113 10770878 ID=MsG0580024850.01.T01:exon:26571;Parent=MsG0580024850.01.T01
Chr5 five_prime_UTR 10771086 10771113 10771086 ID=MsG0580024850.01.T01:five_prime_utr;Parent=MsG0580024850.01.T01
Chr5 CDS 10770878 10771085 10770878 ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5 CDS 10770654 10770781 10770654 ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5 CDS 10770237 10770549 10770237 ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5 CDS 10769643 10769716 10769643 ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5 CDS 10769237 10769512 10769237 ID=MsG0580024850.01.T01:cds;Parent=MsG0580024850.01.T01
Chr5 three_prime_UTR 10768899 10769236 10768899 ID=MsG0580024850.01.T01:three_prime_utr;Parent=MsG0580024850.01.T01
Gene Sequence

>MsG0580024850.01.T01

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCATTTCCTCAACAACGTTGTTCAAACCAACGACATGTCTCAGAAACTTTCAATGCTTGATGACTTAGTTCATAGCTTGGAAGAAGAAATGAAGAAAGTTCTTGCTTTCAAACGTGAACTCCCTCTTTCCATACTCCTCCTTAATGATGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGAAGATTTGAAAGAAAACTGTGATAATAAGAAGAATTGGATGAGTTCAGCTCAGCTATGGACTAATCAAACAAAAACGAACAATGAAGGTGATGATGATAGGATTGTGTTACAGAAGAGTAATGGTGGAGGGGTATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTTAGTTTGGTGTCTGAAGTGAGTCATGGAAATTCAAAGAGTGGTGATGGTAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCGCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCGGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAGCTTGGTGGAGCACATGCCACTCCTAAGCAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCGTCAATTCAGGAGGCTAATAAGTTAGCCTTGTATATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTTTCAAGCCATGGAAGAAACAGTATGGATGCTGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA
Protein sequence

>MsG0580024850.01.T01

MEQQQLNLDLTLASVPKTVSHFLNNVVQTNDMSQKLSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMEDLKENCDNKKNWMSSAQLWTNQTKTNNEGDDDRIVLQKSNGGGVFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGDGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE*