Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025254.01.T01 | MCH92394.1 | 56.944 | 72 | 29 | 2 | 1 | 70 | 47 | 118 | 1.73E-19 | 85.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025254.01.T01 | Q9M8J3 | 50 | 50 | 25 | 0 | 1 | 50 | 32 | 81 | 4.50E-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025254.01.T01 | A0A392N1M6 | 56.944 | 72 | 29 | 2 | 1 | 70 | 47 | 118 | 8.25e-20 | 85.9 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580025254.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025254.01.T01 | MTR_3g005220 | 63.462 | 52 | 16 | 1 | 1 | 52 | 79 | 127 | 2.69e-18 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025254.01.T01 | AT3G06250 | 50.000 | 50 | 25 | 0 | 1 | 50 | 32 | 81 | 4.59e-12 | 59.7 |
| MsG0580025254.01.T01 | AT3G06250 | 50.000 | 50 | 25 | 0 | 1 | 50 | 32 | 81 | 4.59e-12 | 59.7 |
| MsG0580025254.01.T01 | AT3G06250 | 50.000 | 50 | 25 | 0 | 1 | 50 | 32 | 81 | 4.59e-12 | 59.7 |
| MsG0580025254.01.T01 | AT5G18960 | 50.000 | 50 | 25 | 0 | 1 | 50 | 47 | 96 | 1.51e-11 | 58.2 |
| MsG0580025254.01.T01 | AT5G18960 | 50.000 | 50 | 25 | 0 | 1 | 50 | 47 | 96 | 1.51e-11 | 58.2 |
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTTCAAAGGAGGGTTTC+GGG | 0.294231 | 5:+15923288 | MsG0580025254.01.T01:CDS |
| CTTTGTTCAAAGGAGGGTTT+CGG | 0.296294 | 5:+15923287 | MsG0580025254.01.T01:CDS |
| AGAACAGACGGAATGATAAT+TGG | 0.340924 | 5:+15923254 | MsG0580025254.01.T01:CDS |
| AGGCAAGGAATCACTACAAA+AGG | 0.478706 | 5:+15923180 | MsG0580025254.01.T01:CDS |
| TAAAGAGTTTCTTTGTTCAA+AGG | 0.503228 | 5:+15923277 | MsG0580025254.01.T01:CDS |
| GTGATTCCTTGCCTCTTCGA+TGG | 0.520594 | 5:-15923171 | None:intergenic |
| GTGTCAGACATGATGAAACT+AGG | 0.525439 | 5:+15923344 | MsG0580025254.01.T01:CDS |
| AGAGTTTCTTTGTTCAAAGG+AGG | 0.537508 | 5:+15923280 | MsG0580025254.01.T01:CDS |
| GAGTTTCTTTGTTCAAAGGA+GGG | 0.542797 | 5:+15923281 | MsG0580025254.01.T01:CDS |
| CTGTTCTTGATTTAGTAACA+CGG | 0.577898 | 5:-15923238 | None:intergenic |
| AGTAACACGGCCCATGCGAA+AGG | 0.583261 | 5:-15923225 | None:intergenic |
| TGTCAGACATGATGAAACTA+GGG | 0.602315 | 5:+15923345 | MsG0580025254.01.T01:CDS |
| GTTACTAAATCAAGAACAGA+CGG | 0.612323 | 5:+15923242 | MsG0580025254.01.T01:CDS |
| TCACATCCATCGAAGAGGCA+AGG | 0.624553 | 5:+15923165 | MsG0580025254.01.T01:CDS |
| AAAAGGTACGCTAAGAACAA+AGG | 0.638535 | 5:+15923197 | MsG0580025254.01.T01:CDS |
| AGACATGATGAAACTAGGGT+AGG | 0.651192 | 5:+15923349 | MsG0580025254.01.T01:CDS |
| GGAGTTCACATCCATCGAAG+AGG | 0.673976 | 5:+15923160 | MsG0580025254.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAGAGTTTCTTTGTTCAA+AGG | + | Chr5:15923277-15923296 | MsG0580025254.01.T01:CDS | 25.0% |
| CTGTTCTTGATTTAGTAACA+CGG | - | Chr5:15923241-15923260 | None:intergenic | 30.0% | |
| GTTACTAAATCAAGAACAGA+CGG | + | Chr5:15923242-15923261 | MsG0580025254.01.T01:CDS | 30.0% | |
| AGAACAGACGGAATGATAAT+TGG | + | Chr5:15923254-15923273 | MsG0580025254.01.T01:CDS | 35.0% | |
| AGAGTTTCTTTGTTCAAAGG+AGG | + | Chr5:15923280-15923299 | MsG0580025254.01.T01:CDS | 35.0% | |
| GAGTTTCTTTGTTCAAAGGA+GGG | + | Chr5:15923281-15923300 | MsG0580025254.01.T01:CDS | 35.0% | |
| TGTCAGACATGATGAAACTA+GGG | + | Chr5:15923345-15923364 | MsG0580025254.01.T01:CDS | 35.0% | |
| ! | AAAAGGTACGCTAAGAACAA+AGG | + | Chr5:15923197-15923216 | MsG0580025254.01.T01:CDS | 35.0% |
| ! | AAAGGTTTTACCTTTCGCAT+GGG | + | Chr5:15923215-15923234 | MsG0580025254.01.T01:CDS | 35.0% |
| AGGCAAGGAATCACTACAAA+AGG | + | Chr5:15923180-15923199 | MsG0580025254.01.T01:CDS | 40.0% | |
| CTTTGTTCAAAGGAGGGTTT+CGG | + | Chr5:15923287-15923306 | MsG0580025254.01.T01:CDS | 40.0% | |
| GTGTCAGACATGATGAAACT+AGG | + | Chr5:15923344-15923363 | MsG0580025254.01.T01:CDS | 40.0% | |
| TTTGTTCAAAGGAGGGTTTC+GGG | + | Chr5:15923288-15923307 | MsG0580025254.01.T01:CDS | 40.0% | |
| ! | CAAAGGTTTTACCTTTCGCA+TGG | + | Chr5:15923214-15923233 | MsG0580025254.01.T01:CDS | 40.0% |
| GGAGTTCACATCCATCGAAG+AGG | + | Chr5:15923160-15923179 | MsG0580025254.01.T01:CDS | 50.0% | |
| GTGATTCCTTGCCTCTTCGA+TGG | - | Chr5:15923174-15923193 | None:intergenic | 50.0% | |
| TCACATCCATCGAAGAGGCA+AGG | + | Chr5:15923165-15923184 | MsG0580025254.01.T01:CDS | 50.0% | |
| AGTAACACGGCCCATGCGAA+AGG | - | Chr5:15923228-15923247 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 15923158 | 15923370 | 15923158 | ID=MsG0580025254.01;Name=MsG0580025254.01 |
| Chr5 | mRNA | 15923158 | 15923370 | 15923158 | ID=MsG0580025254.01.T01;Parent=MsG0580025254.01;Name=MsG0580025254.01.T01;_AED=0.46;_eAED=0.60;_QI=0|-1|0|1|-1|0|1|0|70 |
| Chr5 | exon | 15923158 | 15923370 | 15923158 | ID=MsG0580025254.01.T01:exon:1797;Parent=MsG0580025254.01.T01 |
| Chr5 | CDS | 15923158 | 15923370 | 15923158 | ID=MsG0580025254.01.T01:cds;Parent=MsG0580025254.01.T01 |
| Gene Sequence |
| Protein sequence |
