Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025576.01.T01 | XP_039684333.1 | 63.71 | 248 | 80 | 2 | 1 | 238 | 1 | 248 | 1.72E-107 | 321 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025576.01.T01 | Q9LKR4 | 31.169 | 154 | 102 | 3 | 86 | 236 | 67 | 219 | 1.38E-16 | 82.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025576.01.T01 | A0A392MH04 | 54.772 | 241 | 58 | 2 | 27 | 267 | 12 | 201 | 1.83e-80 | 256 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580025576.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025576.01.T01 | MTR_5g076070 | 46.753 | 231 | 121 | 1 | 37 | 267 | 18 | 246 | 9.27e-73 | 224 |
| MsG0580025576.01.T01 | MTR_1g070355 | 43.231 | 229 | 130 | 0 | 39 | 267 | 39 | 267 | 1.07e-65 | 214 |
| MsG0580025576.01.T01 | MTR_6g022080 | 46.500 | 200 | 107 | 0 | 39 | 238 | 50 | 249 | 6.17e-65 | 203 |
| MsG0580025576.01.T01 | MTR_8g066350 | 51.337 | 187 | 68 | 2 | 1 | 182 | 1 | 169 | 1.06e-56 | 179 |
| MsG0580025576.01.T01 | MTR_2g101460 | 47.692 | 130 | 68 | 0 | 56 | 185 | 37 | 166 | 1.75e-36 | 129 |
| MsG0580025576.01.T01 | MTR_2g101460 | 48.819 | 127 | 65 | 0 | 59 | 185 | 30 | 156 | 6.69e-36 | 127 |
| MsG0580025576.01.T01 | MTR_6g053140 | 43.949 | 157 | 76 | 3 | 25 | 180 | 58 | 203 | 3.85e-34 | 123 |
| MsG0580025576.01.T01 | MTR_1g052260 | 41.985 | 131 | 67 | 2 | 35 | 156 | 28 | 158 | 1.42e-28 | 107 |
| MsG0580025576.01.T01 | MTR_3g448650 | 48.913 | 92 | 47 | 0 | 59 | 150 | 67 | 158 | 8.28e-28 | 105 |
| MsG0580025576.01.T01 | MTR_0060s0090 | 38.400 | 125 | 77 | 0 | 35 | 159 | 17 | 141 | 1.51e-22 | 91.3 |
| MsG0580025576.01.T01 | MTR_7g446520 | 26.293 | 232 | 148 | 4 | 39 | 267 | 54 | 265 | 3.10e-18 | 83.2 |
| MsG0580025576.01.T01 | MTR_4g050510 | 36.054 | 147 | 65 | 4 | 46 | 192 | 65 | 182 | 1.07e-17 | 79.7 |
| MsG0580025576.01.T01 | MTR_6g463250 | 39.394 | 99 | 45 | 2 | 61 | 159 | 75 | 158 | 5.07e-17 | 77.0 |
| MsG0580025576.01.T01 | MTR_0604s0020 | 48.649 | 74 | 38 | 0 | 85 | 158 | 3 | 76 | 1.00e-16 | 73.2 |
| MsG0580025576.01.T01 | MTR_7g028855 | 27.473 | 182 | 130 | 1 | 34 | 215 | 82 | 261 | 5.45e-16 | 77.8 |
| MsG0580025576.01.T01 | MTR_7g446480 | 31.092 | 119 | 80 | 1 | 151 | 267 | 140 | 258 | 8.00e-14 | 70.5 |
| MsG0580025576.01.T01 | MTR_0402s0040 | 28.655 | 171 | 106 | 5 | 84 | 247 | 36 | 197 | 1.44e-13 | 70.5 |
| MsG0580025576.01.T01 | MTR_2g069720 | 34.286 | 105 | 67 | 1 | 4 | 108 | 29 | 131 | 1.53e-13 | 67.4 |
| MsG0580025576.01.T01 | MTR_7g446510 | 31.092 | 119 | 80 | 1 | 151 | 267 | 155 | 273 | 7.63e-13 | 67.8 |
| MsG0580025576.01.T01 | MTR_6g051425 | 24.599 | 187 | 135 | 4 | 86 | 267 | 29 | 214 | 2.82e-12 | 66.2 |
| MsG0580025576.01.T01 | MTR_8g045760 | 38.462 | 65 | 40 | 0 | 203 | 267 | 1 | 65 | 1.19e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025576.01.T01 | AT5G28530 | 31.169 | 154 | 102 | 3 | 86 | 236 | 67 | 219 | 1.19e-17 | 82.8 |
| MsG0580025576.01.T01 | AT5G28530 | 31.169 | 154 | 102 | 3 | 86 | 236 | 67 | 219 | 1.27e-17 | 82.8 |
| MsG0580025576.01.T01 | AT5G28530 | 31.169 | 154 | 102 | 3 | 86 | 236 | 67 | 219 | 1.41e-17 | 82.8 |
| MsG0580025576.01.T01 | AT5G28530 | 31.169 | 154 | 102 | 3 | 86 | 236 | 67 | 219 | 1.53e-17 | 82.4 |
| MsG0580025576.01.T01 | AT5G28530 | 31.169 | 154 | 102 | 3 | 86 | 236 | 67 | 219 | 1.53e-17 | 82.4 |
| MsG0580025576.01.T01 | AT4G38180 | 28.022 | 182 | 127 | 4 | 89 | 267 | 88 | 268 | 5.74e-13 | 68.9 |
Find 42 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGAGTATAGATGATTTA+TGG | 0.151163 | 5:-20774651 | MsG0580025576.01.T01:CDS |
| AGATGACAAATTATGATATT+TGG | 0.232106 | 5:-20774285 | MsG0580025576.01.T01:CDS |
| GCAACCTCTTCTGTTGTTCC+AGG | 0.291028 | 5:+20774754 | None:intergenic |
| AGCAGACCTTTGTATGTTAT+AGG | 0.299097 | 5:-20774510 | MsG0580025576.01.T01:CDS |
| TTGCTTATTATGTAGGATTT+GGG | 0.330556 | 5:-20774820 | MsG0580025576.01.T01:intron |
| ATACAAAGGTCTGCTGAATT+AGG | 0.354056 | 5:+20774518 | None:intergenic |
| AAGATATTCACACGCGGCTT+TGG | 0.358026 | 5:+20774111 | None:intergenic |
| CCGGAAGCATTCCCTTGAAT+TGG | 0.376341 | 5:+20774317 | None:intergenic |
| CCGCACCTCACACGAGCTCT+TGG | 0.404430 | 5:+20774436 | None:intergenic |
| CCAATTCAAGGGAATGCTTC+CGG | 0.427188 | 5:-20774317 | MsG0580025576.01.T01:CDS |
| GGCAACTTTCGTGAATTGCT+TGG | 0.472098 | 5:-20774701 | MsG0580025576.01.T01:CDS |
| GAGGTTGCAGATGGCACTAA+TGG | 0.478378 | 5:-20774739 | MsG0580025576.01.T01:CDS |
| GGTTTGTTTGCTAATGAAGC+TGG | 0.482853 | 5:-20774214 | MsG0580025576.01.T01:CDS |
| AATGTTGGATGACCAATTCA+AGG | 0.489800 | 5:-20774329 | MsG0580025576.01.T01:CDS |
| TTTGTCATCTTCCTATGTGC+CGG | 0.501947 | 5:+20774298 | None:intergenic |
| CCTTTGTATGTTATAGGCAA+GGG | 0.503564 | 5:-20774504 | MsG0580025576.01.T01:CDS |
| GCCGCAAAAGCAAAGTTGTA+AGG | 0.517061 | 5:-20774555 | MsG0580025576.01.T01:CDS |
| ACCTTTGTATGTTATAGGCA+AGG | 0.523646 | 5:-20774505 | MsG0580025576.01.T01:CDS |
| CACTAATGGTAACAAATTTG+AGG | 0.523859 | 5:-20774725 | MsG0580025576.01.T01:CDS |
| AATGAGCATAGTTATGATCC+TGG | 0.524091 | 5:-20774772 | MsG0580025576.01.T01:CDS |
| GGGAAATTTGTTATTGTCTG+AGG | 0.539782 | 5:-20774800 | MsG0580025576.01.T01:CDS |
| TGAACATAATCATTCAATGT+TGG | 0.554622 | 5:-20774344 | MsG0580025576.01.T01:CDS |
| TTGTTTGCTAATGAAGCTGG+TGG | 0.564693 | 5:-20774211 | MsG0580025576.01.T01:CDS |
| ATGAGCAACATGCGACAAGT+TGG | 0.567690 | 5:-20774259 | MsG0580025576.01.T01:CDS |
| GTGCATATTGATGTAAATAG+TGG | 0.570026 | 5:-20774394 | MsG0580025576.01.T01:CDS |
| TCACCCAGTCGATTCACCAC+AGG | 0.578294 | 5:-20774914 | MsG0580025576.01.T01:intron |
| AGGGAATGCTTCCGGCACAT+AGG | 0.589796 | 5:-20774309 | MsG0580025576.01.T01:CDS |
| CAAACCTGTGGTGAATCGAC+TGG | 0.589959 | 5:+20774910 | None:intergenic |
| ATGTTGGATGACCAATTCAA+GGG | 0.598320 | 5:-20774328 | MsG0580025576.01.T01:CDS |
| CCCTTGCCTATAACATACAA+AGG | 0.598990 | 5:+20774504 | None:intergenic |
| GGGATTAGTAAAGAGAAAAG+TGG | 0.608343 | 5:-20774484 | MsG0580025576.01.T01:CDS |
| ACAACAGAAGAGGTTGCAGA+TGG | 0.645232 | 5:-20774748 | MsG0580025576.01.T01:CDS |
| CCAAGAGCTCGTGTGAGGTG+CGG | 0.650655 | 5:-20774436 | MsG0580025576.01.T01:CDS |
| AAACCTGTGGTGAATCGACT+GGG | 0.650947 | 5:+20774911 | None:intergenic |
| TGATCCTGGAACAACAGAAG+AGG | 0.655464 | 5:-20774758 | MsG0580025576.01.T01:CDS |
| ATATGTACAATGAACAAGAG+AGG | 0.660521 | 5:-20774159 | MsG0580025576.01.T01:CDS |
| TAATGGTAACAAATTTGAGG+AGG | 0.661974 | 5:-20774722 | MsG0580025576.01.T01:CDS |
| AAAGTTGTAAGGAACAACAA+AGG | 0.671963 | 5:-20774544 | MsG0580025576.01.T01:CDS |
| TTGATGTAAATAGTGGTCGG+TGG | 0.686103 | 5:-20774387 | MsG0580025576.01.T01:CDS |
| ATATTGATGTAAATAGTGGT+CGG | 0.715441 | 5:-20774390 | MsG0580025576.01.T01:CDS |
| GTGTGCCAAGAGCTCGTGTG+AGG | 0.742198 | 5:-20774441 | MsG0580025576.01.T01:CDS |
| GCTTTCAAGATATTCACACG+CGG | 0.773852 | 5:+20774105 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATACAAAAAACAAATATA+AGG | + | Chr5:20774145-20774164 | None:intergenic | 15.0% |
| !! | AGATGACAAATTATGATATT+TGG | - | Chr5:20774733-20774752 | MsG0580025576.01.T01:CDS | 20.0% |
| !! | ATTTCAATTGCTTATTATGT+AGG | - | Chr5:20774191-20774210 | MsG0580025576.01.T01:CDS | 20.0% |
| !! | TACAAAAAACAAATATAAGG+AGG | + | Chr5:20774142-20774161 | None:intergenic | 20.0% |
| ! | AGTTGAGTATAGATGATTTA+TGG | - | Chr5:20774367-20774386 | MsG0580025576.01.T01:CDS | 25.0% |
| ! | ATATTGATGTAAATAGTGGT+CGG | - | Chr5:20774628-20774647 | MsG0580025576.01.T01:CDS | 25.0% |
| ! | ATTGCTTATTATGTAGGATT+TGG | - | Chr5:20774197-20774216 | MsG0580025576.01.T01:CDS | 25.0% |
| ! | TAGCAAACAAACCAAAAATA+TGG | + | Chr5:20774797-20774816 | None:intergenic | 25.0% |
| ! | TGAACATAATCATTCAATGT+TGG | - | Chr5:20774674-20774693 | MsG0580025576.01.T01:CDS | 25.0% |
| ! | TTGCTTATTATGTAGGATTT+GGG | - | Chr5:20774198-20774217 | MsG0580025576.01.T01:CDS | 25.0% |
| !! | AGATATCATTTTGTAGATGT+TGG | - | Chr5:20774390-20774409 | MsG0580025576.01.T01:CDS | 25.0% |
| !! | ATCAAGACAAACCATATTTT+TGG | - | Chr5:20774783-20774802 | MsG0580025576.01.T01:CDS | 25.0% |
| !!! | GATTTCAAAAAGTTGGTTTT+CGG | - | Chr5:20774829-20774848 | MsG0580025576.01.T01:intron | 25.0% |
| AAAGTTGTAAGGAACAACAA+AGG | - | Chr5:20774474-20774493 | MsG0580025576.01.T01:CDS | 30.0% | |
| ATATGTACAATGAACAAGAG+AGG | - | Chr5:20774859-20774878 | MsG0580025576.01.T01:intron | 30.0% | |
| CACTAATGGTAACAAATTTG+AGG | - | Chr5:20774293-20774312 | MsG0580025576.01.T01:CDS | 30.0% | |
| GTGCATATTGATGTAAATAG+TGG | - | Chr5:20774624-20774643 | MsG0580025576.01.T01:CDS | 30.0% | |
| TAATGGTAACAAATTTGAGG+AGG | - | Chr5:20774296-20774315 | MsG0580025576.01.T01:CDS | 30.0% | |
| ! | TGGCGTTTCTATTTTACAAT+TGG | - | Chr5:20774415-20774434 | MsG0580025576.01.T01:CDS | 30.0% |
| AATGAGCATAGTTATGATCC+TGG | - | Chr5:20774246-20774265 | MsG0580025576.01.T01:CDS | 35.0% | |
| AATGTTGGATGACCAATTCA+AGG | - | Chr5:20774689-20774708 | MsG0580025576.01.T01:CDS | 35.0% | |
| ACCTTTGTATGTTATAGGCA+AGG | - | Chr5:20774513-20774532 | MsG0580025576.01.T01:CDS | 35.0% | |
| AGCAGACCTTTGTATGTTAT+AGG | - | Chr5:20774508-20774527 | MsG0580025576.01.T01:CDS | 35.0% | |
| ATACAAAGGTCTGCTGAATT+AGG | + | Chr5:20774503-20774522 | None:intergenic | 35.0% | |
| ATGTTGGATGACCAATTCAA+GGG | - | Chr5:20774690-20774709 | MsG0580025576.01.T01:CDS | 35.0% | |
| CCTTTGTATGTTATAGGCAA+GGG | - | Chr5:20774514-20774533 | MsG0580025576.01.T01:CDS | 35.0% | |
| ! | AATTGGTATGCGAGTTTTCA+TGG | - | Chr5:20774432-20774451 | MsG0580025576.01.T01:CDS | 35.0% |
| ! | ATTGGTATGCGAGTTTTCAT+GGG | - | Chr5:20774433-20774452 | MsG0580025576.01.T01:CDS | 35.0% |
| ! | GGGATTAGTAAAGAGAAAAG+TGG | - | Chr5:20774534-20774553 | MsG0580025576.01.T01:CDS | 35.0% |
| ! | TCATTTTGTAGATGTTGGTG+TGG | - | Chr5:20774395-20774414 | MsG0580025576.01.T01:CDS | 35.0% |
| !! | GGGAAATTTGTTATTGTCTG+AGG | - | Chr5:20774218-20774237 | MsG0580025576.01.T01:CDS | 35.0% |
| !! | TCCTTACAACTTTGCTTTTG+CGG | + | Chr5:20774467-20774486 | None:intergenic | 35.0% |
| CCCTTGCCTATAACATACAA+AGG | + | Chr5:20774517-20774536 | None:intergenic | 40.0% | |
| GCTTTCAAGATATTCACACG+CGG | + | Chr5:20774916-20774935 | None:intergenic | 40.0% | |
| TTGATGTAAATAGTGGTCGG+TGG | - | Chr5:20774631-20774650 | MsG0580025576.01.T01:CDS | 40.0% | |
| TTTGTCATCTTCCTATGTGC+CGG | + | Chr5:20774723-20774742 | None:intergenic | 40.0% | |
| ! | GGTTTGTTTGCTAATGAAGC+TGG | - | Chr5:20774804-20774823 | MsG0580025576.01.T01:CDS | 40.0% |
| ! | TTGTTTGCTAATGAAGCTGG+TGG | - | Chr5:20774807-20774826 | MsG0580025576.01.T01:CDS | 40.0% |
| !! | GCTGGTGGATTTCAAAAAGT+TGG | - | Chr5:20774822-20774841 | MsG0580025576.01.T01:intron | 40.0% |
| AAACCTGTGGTGAATCGACT+GGG | + | Chr5:20774110-20774129 | None:intergenic | 45.0% | |
| AAGATATTCACACGCGGCTT+TGG | + | Chr5:20774910-20774929 | None:intergenic | 45.0% | |
| ACAACAGAAGAGGTTGCAGA+TGG | - | Chr5:20774270-20774289 | MsG0580025576.01.T01:CDS | 45.0% | |
| ATGAGCAACATGCGACAAGT+TGG | - | Chr5:20774759-20774778 | MsG0580025576.01.T01:CDS | 45.0% | |
| CCAATTCAAGGGAATGCTTC+CGG | - | Chr5:20774701-20774720 | MsG0580025576.01.T01:CDS | 45.0% | |
| GAGGAGACAATACAAACCTG+TGG | + | Chr5:20774123-20774142 | None:intergenic | 45.0% | |
| GCCGCAAAAGCAAAGTTGTA+AGG | - | Chr5:20774463-20774482 | MsG0580025576.01.T01:CDS | 45.0% | |
| GGCAACTTTCGTGAATTGCT+TGG | - | Chr5:20774317-20774336 | MsG0580025576.01.T01:CDS | 45.0% | |
| TGATCCTGGAACAACAGAAG+AGG | - | Chr5:20774260-20774279 | MsG0580025576.01.T01:CDS | 45.0% | |
| CAAACCTGTGGTGAATCGAC+TGG | + | Chr5:20774111-20774130 | None:intergenic | 50.0% | |
| GAGGTTGCAGATGGCACTAA+TGG | - | Chr5:20774279-20774298 | MsG0580025576.01.T01:CDS | 50.0% | |
| GCAACCTCTTCTGTTGTTCC+AGG | + | Chr5:20774267-20774286 | None:intergenic | 50.0% | |
| ! | GAGCTCTTGGCACACGTTTT+CGG | + | Chr5:20774572-20774591 | None:intergenic | 50.0% |
| ! | GCGAGTTTTCATGGGTTTGC+TGG | - | Chr5:20774441-20774460 | MsG0580025576.01.T01:CDS | 50.0% |
| !! | ACACGTTTTCGGCTTGTGGA+TGG | + | Chr5:20774561-20774580 | None:intergenic | 50.0% |
| !! | CACGTTTTCGGCTTGTGGAT+GGG | + | Chr5:20774560-20774579 | None:intergenic | 50.0% |
| !! | CCGGAAGCATTCCCTTGAAT+TGG | + | Chr5:20774704-20774723 | None:intergenic | 50.0% |
| AGGGAATGCTTCCGGCACAT+AGG | - | Chr5:20774709-20774728 | MsG0580025576.01.T01:CDS | 55.0% | |
| ! | TCACCCAGTCGATTCACCAC+AGG | - | Chr5:20774104-20774123 | MsG0580025576.01.T01:CDS | 55.0% |
| !! | TGGCACACGTTTTCGGCTTG+TGG | + | Chr5:20774565-20774584 | None:intergenic | 55.0% |
| CCAAGAGCTCGTGTGAGGTG+CGG | - | Chr5:20774582-20774601 | MsG0580025576.01.T01:CDS | 60.0% | |
| GTGTGCCAAGAGCTCGTGTG+AGG | - | Chr5:20774577-20774596 | MsG0580025576.01.T01:CDS | 60.0% | |
| CCGCACCTCACACGAGCTCT+TGG | + | Chr5:20774585-20774604 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20774075 | 20774965 | 20774075 | ID=MsG0580025576.01;Name=MsG0580025576.01 |
| Chr5 | mRNA | 20774075 | 20774965 | 20774075 | ID=MsG0580025576.01.T01;Parent=MsG0580025576.01;Name=MsG0580025576.01.T01;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|2|0|267 |
| Chr5 | exon | 20774915 | 20774965 | 20774915 | ID=MsG0580025576.01.T01:exon:23354;Parent=MsG0580025576.01.T01 |
| Chr5 | exon | 20774075 | 20774827 | 20774075 | ID=MsG0580025576.01.T01:exon:23353;Parent=MsG0580025576.01.T01 |
| Chr5 | CDS | 20774915 | 20774965 | 20774915 | ID=MsG0580025576.01.T01:cds;Parent=MsG0580025576.01.T01 |
| Chr5 | CDS | 20774075 | 20774827 | 20774075 | ID=MsG0580025576.01.T01:cds;Parent=MsG0580025576.01.T01 |
| Gene Sequence |
| Protein sequence |
