AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025881.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025881.01.T01 MTR_1g035490 60.776 232 74 5 9 230 9 233 2.27e-83 254
MsG0580025881.01.T01 MTR_1g035570 61.009 218 55 4 1 199 91 297 1.01e-72 229
MsG0580025881.01.T01 MTR_1g035640 43.028 251 124 6 1 239 91 334 6.58e-52 177
MsG0580025881.01.T01 MTR_1g109310 81.579 76 14 0 231 306 1 76 3.09e-39 133
MsG0580025881.01.T01 MTR_7g057130 77.500 80 18 0 227 306 71 150 7.56e-37 129
MsG0580025881.01.T01 MTR_5g026700 76.543 81 19 0 226 306 109 189 5.42e-36 128
MsG0580025881.01.T01 MTR_2g093330 76.543 81 19 0 226 306 109 189 1.86e-35 127
MsG0580025881.01.T01 MTR_2g449870 81.818 77 14 0 230 306 518 594 9.51e-35 133
MsG0580025881.01.T01 MTR_1g037100 74.074 81 21 0 226 306 109 189 6.60e-34 123
MsG0580025881.01.T01 MTR_5g089410 75.676 74 18 0 233 306 119 192 7.38e-32 117
MsG0580025881.01.T01 MTR_1g035620 41.304 230 107 12 1 221 95 305 2.08e-30 119
MsG0580025881.01.T01 MTR_1g050845 41.000 200 84 7 1 191 95 269 1.98e-26 109
MsG0580025881.01.T01 MTR_7g007740 76.623 77 13 1 235 306 110 186 7.92e-26 101
MsG0580025881.01.T01 MTR_6g085030 80.392 51 8 1 226 274 50 100 1.75e-19 82.0
MsG0580025881.01.T01 MTR_6g061070 31.624 234 114 7 1 198 103 326 3.02e-18 85.1
MsG0580025881.01.T01 MTR_3g067605 39.860 143 72 4 170 306 9 143 1.78e-17 78.2
MsG0580025881.01.T01 MTR_4g115840 67.925 53 11 1 235 281 3 55 6.24e-15 68.6
MsG0580025881.01.T01 MTR_7g061690 75.000 40 10 0 267 306 50 89 4.86e-13 64.3
MsG0580025881.01.T01 MTR_3g014220 78.378 37 8 0 230 266 165 201 6.48e-12 64.7
MsG0580025881.01.T01 MTR_5g044670 27.556 225 146 8 1 212 104 324 6.47e-11 62.8
MsG0580025881.01.T01 MTR_2g055480 79.412 34 3 1 231 260 1 34 9.51e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCCTGCGGCACCTGCTTT+TGG 0.252053 5:+25878392 None:intergenic
GTAGTGGTTGGAAAATATTC+AGG 0.287827 5:-25879506 MsG0580025881.01.T01:CDS
TAACGCCAATGGCTATAATT+TGG 0.309691 5:-25879732 MsG0580025881.01.T01:intron
ATAAAGGGCCTGCAACAATA+TGG 0.324459 5:-25879596 MsG0580025881.01.T01:CDS
ACATTGGGGAAACTTCTTCT+AGG 0.333835 5:-25879191 MsG0580025881.01.T01:CDS
CTTATATTGTTAGTGATGTT+TGG 0.354932 5:-25880444 None:intergenic
TTACCAACCCATATTGTTGC+AGG 0.356512 5:+25879588 None:intergenic
TCAGAATCAGGGTAACGTTC+AGG 0.362410 5:-25878561 MsG0580025881.01.T01:CDS
GAACACTTGATGGATTATAA+AGG 0.363316 5:-25879612 MsG0580025881.01.T01:CDS
CATGTCTCAGAATCAGAATC+AGG 0.366260 5:-25878573 MsG0580025881.01.T01:CDS
CCTGGTAATTTCTTAGGGCT+TGG 0.374720 5:+25879384 None:intergenic
CACTACTCATAATAGATCTT+TGG 0.378317 5:+25879523 None:intergenic
TCATTAACAGTTCCAGATGT+TGG 0.400715 5:+25879788 None:intergenic
TGTTCTCTTGAAAACTTACT+TGG 0.404641 5:+25879630 None:intergenic
CATTAACAGTTCCAGATGTT+GGG 0.406126 5:+25879789 None:intergenic
ATGGCTGTGGAAGTGAGCTT+GGG 0.424295 5:-25879561 MsG0580025881.01.T01:CDS
CAAACTATTCATAAGTCAAT+CGG 0.427954 5:+25878356 None:intergenic
ACTATTACTCGTGGCAGAAA+TGG 0.435968 5:-25879408 MsG0580025881.01.T01:CDS
AACAAAGCAATCTCAAAGAA+AGG 0.443342 5:-25880295 MsG0580025881.01.T01:CDS
TTGTAAAGAAGAAAGACATT+GGG 0.450727 5:-25879206 MsG0580025881.01.T01:CDS
GGTGCCGCAGGGACTGTAGC+AGG 0.453118 5:-25878383 MsG0580025881.01.T01:CDS
AACACTTGATGGATTATAAA+GGG 0.456345 5:-25879611 MsG0580025881.01.T01:CDS
AATATGGGTTGGTAAGGAAA+TGG 0.458034 5:-25879580 MsG0580025881.01.T01:CDS
CAACCAAAAGCAGGTGCCGC+AGG 0.460798 5:-25878395 MsG0580025881.01.T01:CDS
GCTAGAAAATACCCAACATC+TGG 0.468222 5:-25879800 MsG0580025881.01.T01:CDS
AATGGCTGTGGAAGTGAGCT+TGG 0.471175 5:-25879562 MsG0580025881.01.T01:CDS
AGACGAGGTTGAGGAGGATC+GGG 0.484277 5:-25878439 MsG0580025881.01.T01:CDS
GTGATTCTGTGCCATGTCAT+AGG 0.485204 5:+25878512 None:intergenic
TAAAGGGCCTGCAACAATAT+GGG 0.501208 5:-25879595 MsG0580025881.01.T01:CDS
TGCAACAATATGGGTTGGTA+AGG 0.502211 5:-25879586 MsG0580025881.01.T01:CDS
CCAAGCCCTAAGAAATTACC+AGG 0.507995 5:-25879384 MsG0580025881.01.T01:intron
ATGAAGAGAGTGATGAATTG+AGG 0.511380 5:-25880344 MsG0580025881.01.T01:CDS
AAACTATTCATAAGTCAATC+GGG 0.512752 5:+25878357 None:intergenic
CTTGTAAAGAAGAAAGACAT+TGG 0.513890 5:-25879207 MsG0580025881.01.T01:CDS
TGAAGAGAGTGATGAATTGA+GGG 0.515870 5:-25880343 MsG0580025881.01.T01:CDS
ACAAAGCAATCTCAAAGAAA+GGG 0.516491 5:-25880294 MsG0580025881.01.T01:CDS
ACTTACCAAATTATAGCCAT+TGG 0.516643 5:+25879727 None:intergenic
GATCTATTATGAGTAGTGGT+TGG 0.518269 5:-25879518 MsG0580025881.01.T01:CDS
CAGAATCAGGGTAACGTTCA+GGG 0.519696 5:-25878560 MsG0580025881.01.T01:CDS
CAGACGAGGTTGAGGAGGAT+CGG 0.526215 5:-25878440 MsG0580025881.01.T01:CDS
ACTACCCAACCAAAATTCTC+AGG 0.529908 5:-25880243 MsG0580025881.01.T01:intron
AATATAAAATCAGTTGATGA+AGG 0.529972 5:-25880393 MsG0580025881.01.T01:CDS
GGTTGGTAAGGAAATGGCTG+TGG 0.531845 5:-25879574 MsG0580025881.01.T01:CDS
CTGATTGTACACCGTTACCT+TGG 0.532434 5:+25878460 None:intergenic
GTAAATTTGAGATGATTCAT+CGG 0.551503 5:-25879452 MsG0580025881.01.T01:CDS
TAGTGGTTGGAAAATATTCA+GGG 0.551832 5:-25879505 MsG0580025881.01.T01:CDS
ATAGTAACAGTGAATGATGC+AGG 0.558530 5:+25879426 None:intergenic
ACTCATAATAGATCTTTGGC+AGG 0.560871 5:+25879527 None:intergenic
TGTACAATCAGACGAGGTTG+AGG 0.574442 5:-25878448 MsG0580025881.01.T01:CDS
CAAAGATCTATTATGAGTAG+TGG 0.575571 5:-25879522 MsG0580025881.01.T01:CDS
ATCGGGAGGACATACTACTT+TGG 0.578248 5:-25878422 MsG0580025881.01.T01:CDS
AACCAAAAGCAGGTGCCGCA+GGG 0.581033 5:-25878394 MsG0580025881.01.T01:CDS
TTTGGTCCTCAACCAAAAGC+AGG 0.584671 5:-25878404 MsG0580025881.01.T01:CDS
GGTGCTGAAGACACAGAAGA+AGG 0.585468 5:-25880372 MsG0580025881.01.T01:CDS
AAAGAAGGATTCCAAGGTAA+CGG 0.588911 5:-25878471 MsG0580025881.01.T01:CDS
GGGCCTGCAACAATATGGGT+TGG 0.600063 5:-25879591 MsG0580025881.01.T01:CDS
ATGTCTCAGAATCAGAATCA+GGG 0.611412 5:-25878572 MsG0580025881.01.T01:CDS
TAATAAAATAGCCATGGTGA+AGG 0.612626 5:-25879825 MsG0580025881.01.T01:CDS
TGGCACAGAATCACATGTGA+TGG 0.613628 5:-25878503 MsG0580025881.01.T01:CDS
TAAAATAGCCATGGTGAAGG+CGG 0.617629 5:-25879822 MsG0580025881.01.T01:CDS
CGAGGTTGAGGAGGATCGGG+AGG 0.628914 5:-25878436 MsG0580025881.01.T01:CDS
TTGTCACATCATAACGCCAA+TGG 0.632543 5:-25879743 MsG0580025881.01.T01:CDS
TTCACTGTTACTATTACTCG+TGG 0.635895 5:-25879417 MsG0580025881.01.T01:CDS
AGGTAGTAATAAAATAGCCA+TGG 0.642448 5:-25879831 MsG0580025881.01.T01:CDS
TGGCTGTGGAAGTGAGCTTG+GGG 0.650161 5:-25879560 MsG0580025881.01.T01:CDS
TGTAAAGAAGAAAGACATTG+GGG 0.650633 5:-25879205 MsG0580025881.01.T01:CDS
ACAATCAGACGAGGTTGAGG+AGG 0.658511 5:-25878445 MsG0580025881.01.T01:CDS
GAGGGATGAGAGTTCATACG+AGG 0.738856 5:-25880325 MsG0580025881.01.T01:CDS
TAACGGTGTACAATCAGACG+AGG 0.741001 5:-25878454 MsG0580025881.01.T01:CDS
GTTGCCTGCTACAGTCCCTG+CGG 0.745085 5:+25878379 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATAATTACTTTTTTTTA+AGG - Chr5:25878690-25878709 MsG0580025881.01.T01:intron 10.0%
!! TTAAAATTGTTAGAATTGTT+AGG - Chr5:25879925-25879944 MsG0580025881.01.T01:intron 15.0%
!!! GATTATTTTTAGAAGATTTT+AGG + Chr5:25878899-25878918 None:intergenic 15.0%
!!! GGTTTATGTTTTATAATTTT+AGG - Chr5:25879946-25879965 MsG0580025881.01.T01:intron 15.0%
!!! GTATTTAAAATTAAAGCATA+AGG + Chr5:25879779-25879798 None:intergenic 15.0%
!!! TTATAATTTTAGGATAGTTT+AGG - Chr5:25879956-25879975 MsG0580025881.01.T01:intron 15.0%
!!! TTTTTTTTTCTCTTTTCATT+TGG - Chr5:25879698-25879717 MsG0580025881.01.T01:intron 15.0%
!! AAATTCATAGATACTTTGTA+TGG + Chr5:25879095-25879114 None:intergenic 20.0%
!! CTAAACATAAAACTAAATAC+TGG + Chr5:25879872-25879891 None:intergenic 20.0%
!! GACAATGTAATTTCAAAAAA+GGG + Chr5:25879035-25879054 None:intergenic 20.0%
!! TAGAATTGTTAGAATTGTTT+AGG - Chr5:25880024-25880043 MsG0580025881.01.T01:intron 20.0%
!! TGACAATGTAATTTCAAAAA+AGG + Chr5:25879036-25879055 None:intergenic 20.0%
!! TGTTAGAATTGTTTAGATTT+AGG - Chr5:25880091-25880110 MsG0580025881.01.T01:intron 20.0%
!! TTAGAATTGTTAGAATTGTT+AGG - Chr5:25879979-25879998 MsG0580025881.01.T01:intron 20.0%
!!! AATATAAAATCAGTTGATGA+AGG - Chr5:25878400-25878419 MsG0580025881.01.T01:CDS 20.0%
!!! ATGAATAGTTTATGAGTTTT+TGG - Chr5:25879732-25879751 MsG0580025881.01.T01:intron 20.0%
!!! ATTTCATTGTTGTTTAGTTT+AGG - Chr5:25880168-25880187 MsG0580025881.01.T01:intron 20.0%
!!! CAGTATTTAGTTTTATGTTT+AGG - Chr5:25879870-25879889 MsG0580025881.01.T01:intron 20.0%
!!! TTCATCAACTGATTTTATAT+TGG + Chr5:25878401-25878420 None:intergenic 20.0%
!!! TTTTCTCTACAGATAATTTT+TGG - Chr5:25878811-25878830 MsG0580025881.01.T01:intron 20.0%
! AAAAACCTGGTAATTTCTTA+GGG + Chr5:25879417-25879436 None:intergenic 25.0%
! AAAAGTCATTTCAAATTCAC+TGG + Chr5:25879801-25879820 None:intergenic 25.0%
! AATTGTTTAGATTTAGGTGT+AGG - Chr5:25880097-25880116 MsG0580025881.01.T01:intron 25.0%
! ACTAAATACTGGATAAGTTT+AGG + Chr5:25879861-25879880 None:intergenic 25.0%
! ATTACTCATTAGCTAGATTA+AGG - Chr5:25878623-25878642 MsG0580025881.01.T01:intron 25.0%
! CATGACATGTTATAAAAAAG+GGG - Chr5:25879546-25879565 MsG0580025881.01.T01:CDS 25.0%
! CCATGACATGTTATAAAAAA+GGG - Chr5:25879545-25879564 MsG0580025881.01.T01:CDS 25.0%
! TCCATGACATGTTATAAAAA+AGG - Chr5:25879544-25879563 MsG0580025881.01.T01:CDS 25.0%
! TTGTAAAGAAGAAAGACATT+GGG - Chr5:25879587-25879606 MsG0580025881.01.T01:CDS 25.0%
! TTGTTAGAATTGTTAGGTTT+AGG - Chr5:25879931-25879950 MsG0580025881.01.T01:intron 25.0%
!! CAAGAAAATTTTCTTTTGGA+CGG + Chr5:25879630-25879649 None:intergenic 25.0%
!! CCAAAAGAAAATTTTCTTGA+GGG - Chr5:25879631-25879650 MsG0580025881.01.T01:CDS 25.0%
!! GTAAATTTGAGATGATTCAT+CGG - Chr5:25879341-25879360 MsG0580025881.01.T01:intron 25.0%
!! TAAACTATGATTTGAAACAG+TGG - Chr5:25879137-25879156 MsG0580025881.01.T01:intron 25.0%
!! TCCAAAAGAAAATTTTCTTG+AGG - Chr5:25879630-25879649 MsG0580025881.01.T01:CDS 25.0%
!! TGTTAGAATTGTTTAGGTTT+AGG - Chr5:25880030-25880049 MsG0580025881.01.T01:intron 25.0%
!!! AACACTTGATGGATTATAAA+GGG - Chr5:25879182-25879201 MsG0580025881.01.T01:CDS 25.0%
!!! TTTTGTGATAGTTTAGGTTT+AGG - Chr5:25880121-25880140 MsG0580025881.01.T01:intron 25.0%
AACAAAGCAATCTCAAAGAA+AGG - Chr5:25878498-25878517 MsG0580025881.01.T01:CDS 30.0%
AAGAAAGGGAAAAAGAAAAG+TGG - Chr5:25878513-25878532 MsG0580025881.01.T01:CDS 30.0%
ACAAAGCAATCTCAAAGAAA+GGG - Chr5:25878499-25878518 MsG0580025881.01.T01:CDS 30.0%
ACTTACCAAATTATAGCCAT+TGG + Chr5:25879069-25879088 None:intergenic 30.0%
AGAAAGGGAAAAAGAAAAGT+GGG - Chr5:25878514-25878533 MsG0580025881.01.T01:CDS 30.0%
AGGTAGTAATAAAATAGCCA+TGG - Chr5:25878962-25878981 MsG0580025881.01.T01:intron 30.0%
CAAAGATCTATTATGAGTAG+TGG - Chr5:25879271-25879290 MsG0580025881.01.T01:intron 30.0%
CACTACTCATAATAGATCTT+TGG + Chr5:25879273-25879292 None:intergenic 30.0%
CTTGTAAAGAAGAAAGACAT+TGG - Chr5:25879586-25879605 MsG0580025881.01.T01:CDS 30.0%
GAAAAACCTGGTAATTTCTT+AGG + Chr5:25879418-25879437 None:intergenic 30.0%
GAAAGAAAGAAAGAAAAACC+TGG + Chr5:25879430-25879449 None:intergenic 30.0%
TAATAAAATAGCCATGGTGA+AGG - Chr5:25878968-25878987 MsG0580025881.01.T01:intron 30.0%
TAGTGGTTGGAAAATATTCA+GGG - Chr5:25879288-25879307 MsG0580025881.01.T01:intron 30.0%
TATAAAAAAGGGGTCTCTTA+TGG - Chr5:25879556-25879575 MsG0580025881.01.T01:CDS 30.0%
TATTACTACCTGCAAAGTTT+AGG + Chr5:25878953-25878972 None:intergenic 30.0%
TGTAAAGAAGAAAGACATTG+GGG - Chr5:25879588-25879607 MsG0580025881.01.T01:CDS 30.0%
TGTTCTCTTGAAAACTTACT+TGG + Chr5:25879166-25879185 None:intergenic 30.0%
! AATTGTTTAGGTTTAGGTGT+AGG - Chr5:25880036-25880055 MsG0580025881.01.T01:intron 30.0%
! CCCTCAAGAAAATTTTCTTT+TGG + Chr5:25879634-25879653 None:intergenic 30.0%
! CTAAAACTGCTTCTCATTTT+AGG + Chr5:25878787-25878806 None:intergenic 30.0%
! GAAAAATCACCTGAGAATTT+TGG + Chr5:25878562-25878581 None:intergenic 30.0%
! GATAGTTTAGGTTTAGGTTT+AGG - Chr5:25880127-25880146 MsG0580025881.01.T01:intron 30.0%
! GTAGGTTTTTGTGATAGTTT+AGG - Chr5:25880115-25880134 MsG0580025881.01.T01:intron 30.0%
!! GAACACTTGATGGATTATAA+AGG - Chr5:25879181-25879200 MsG0580025881.01.T01:CDS 30.0%
!! GTAGGTTTTGATGATAGTTT+AGG - Chr5:25880054-25880073 MsG0580025881.01.T01:intron 30.0%
!!! CCCTTTTTTATAACATGTCA+TGG + Chr5:25879548-25879567 None:intergenic 30.0%
AAAGAAGGATTCCAAGGTAA+CGG - Chr5:25880322-25880341 MsG0580025881.01.T01:CDS 35.0%
AAGAAAAATCGCCTATGACA+TGG - Chr5:25880270-25880289 MsG0580025881.01.T01:CDS 35.0%
ACTCATAATAGATCTTTGGC+AGG + Chr5:25879269-25879288 None:intergenic 35.0%
AGTTAATGCCTAAACTTTGC+AGG - Chr5:25878942-25878961 MsG0580025881.01.T01:intron 35.0%
ATAGTAACAGTGAATGATGC+AGG + Chr5:25879370-25879389 None:intergenic 35.0%
ATGAAGAGAGTGATGAATTG+AGG - Chr5:25878449-25878468 MsG0580025881.01.T01:CDS 35.0%
ATGTCTCAGAATCAGAATCA+GGG - Chr5:25880221-25880240 MsG0580025881.01.T01:intron 35.0%
CATTAACAGTTCCAGATGTT+GGG + Chr5:25879007-25879026 None:intergenic 35.0%
GATCTATTATGAGTAGTGGT+TGG - Chr5:25879275-25879294 MsG0580025881.01.T01:intron 35.0%
GTAGTGGTTGGAAAATATTC+AGG - Chr5:25879287-25879306 MsG0580025881.01.T01:intron 35.0%
TAACGCCAATGGCTATAATT+TGG - Chr5:25879061-25879080 MsG0580025881.01.T01:intron 35.0%
TCATTAACAGTTCCAGATGT+TGG + Chr5:25879008-25879027 None:intergenic 35.0%
TGAAGAGAGTGATGAATTGA+GGG - Chr5:25878450-25878469 MsG0580025881.01.T01:CDS 35.0%
TGTCAAAAAGAAGGATTCCA+AGG - Chr5:25880316-25880335 MsG0580025881.01.T01:CDS 35.0%
TTCACTGTTACTATTACTCG+TGG - Chr5:25879376-25879395 MsG0580025881.01.T01:intron 35.0%
! TGATGGTTCTGTCAAAAAGA+AGG - Chr5:25880307-25880326 MsG0580025881.01.T01:CDS 35.0%
!! AATATGGGTTGGTAAGGAAA+TGG - Chr5:25879213-25879232 MsG0580025881.01.T01:CDS 35.0%
!! AATCACCTGAGAATTTTGGT+TGG + Chr5:25878558-25878577 None:intergenic 35.0%
!! GTTTTCAAGAGAACACTTGA+TGG - Chr5:25879171-25879190 MsG0580025881.01.T01:CDS 35.0%
!!! ATCACCTGAGAATTTTGGTT+GGG + Chr5:25878557-25878576 None:intergenic 35.0%
ACATTGGGGAAACTTCTTCT+AGG - Chr5:25879602-25879621 MsG0580025881.01.T01:CDS 40.0%
ACTACCCAACCAAAATTCTC+AGG - Chr5:25878550-25878569 MsG0580025881.01.T01:CDS 40.0%
ACTATTACTCGTGGCAGAAA+TGG - Chr5:25879385-25879404 MsG0580025881.01.T01:CDS 40.0%
ATAAAGGGCCTGCAACAATA+TGG - Chr5:25879197-25879216 MsG0580025881.01.T01:CDS 40.0%
CATGTCTCAGAATCAGAATC+AGG - Chr5:25880220-25880239 MsG0580025881.01.T01:intron 40.0%
GCTAGAAAATACCCAACATC+TGG - Chr5:25878993-25879012 MsG0580025881.01.T01:intron 40.0%
TAAAATAGCCATGGTGAAGG+CGG - Chr5:25878971-25878990 MsG0580025881.01.T01:intron 40.0%
TAAAGGGCCTGCAACAATAT+GGG - Chr5:25879198-25879217 MsG0580025881.01.T01:CDS 40.0%
TTACCAACCCATATTGTTGC+AGG + Chr5:25879208-25879227 None:intergenic 40.0%
TTGTCACATCATAACGCCAA+TGG - Chr5:25879050-25879069 MsG0580025881.01.T01:intron 40.0%
!! TGCAACAATATGGGTTGGTA+AGG - Chr5:25879207-25879226 MsG0580025881.01.T01:CDS 40.0%
ATCGGGAGGACATACTACTT+TGG - Chr5:25880371-25880390 MsG0580025881.01.T01:CDS 45.0%
CAGAATCAGGGTAACGTTCA+GGG - Chr5:25880233-25880252 MsG0580025881.01.T01:intron 45.0%
CCAAGCCCTAAGAAATTACC+AGG - Chr5:25879409-25879428 MsG0580025881.01.T01:CDS 45.0%
CCTGGTAATTTCTTAGGGCT+TGG + Chr5:25879412-25879431 None:intergenic 45.0%
CTGATTGTACACCGTTACCT+TGG + Chr5:25880336-25880355 None:intergenic 45.0%
GTGATTCTGTGCCATGTCAT+AGG + Chr5:25880284-25880303 None:intergenic 45.0%
TAACGGTGTACAATCAGACG+AGG - Chr5:25880339-25880358 MsG0580025881.01.T01:CDS 45.0%
TCAGAATCAGGGTAACGTTC+AGG - Chr5:25880232-25880251 MsG0580025881.01.T01:intron 45.0%
TGGCACAGAATCACATGTGA+TGG - Chr5:25880290-25880309 MsG0580025881.01.T01:CDS 45.0%
TGTACAATCAGACGAGGTTG+AGG - Chr5:25880345-25880364 MsG0580025881.01.T01:CDS 45.0%
TTTGGTCCTCAACCAAAAGC+AGG - Chr5:25880389-25880408 MsG0580025881.01.T01:CDS 45.0%
!!! TATGATAAAAAAATTATTTT+TGG - Chr5:25878869-25878888 MsG0580025881.01.T01:intron 5.0%
AATGGCTGTGGAAGTGAGCT+TGG - Chr5:25879231-25879250 MsG0580025881.01.T01:intron 50.0%
ACAATCAGACGAGGTTGAGG+AGG - Chr5:25880348-25880367 MsG0580025881.01.T01:CDS 50.0%
ATGGCTGTGGAAGTGAGCTT+GGG - Chr5:25879232-25879251 MsG0580025881.01.T01:intron 50.0%
GAGGGATGAGAGTTCATACG+AGG - Chr5:25878468-25878487 MsG0580025881.01.T01:CDS 50.0%
! GGTGCTGAAGACACAGAAGA+AGG - Chr5:25878421-25878440 MsG0580025881.01.T01:CDS 50.0%
!! GGTTGGTAAGGAAATGGCTG+TGG - Chr5:25879219-25879238 MsG0580025881.01.T01:CDS 50.0%
!! TTTTCTAGCCGCCTTCACCA+TGG + Chr5:25878982-25879001 None:intergenic 50.0%
AGACGAGGTTGAGGAGGATC+GGG - Chr5:25880354-25880373 MsG0580025881.01.T01:CDS 55.0%
CAGACGAGGTTGAGGAGGAT+CGG - Chr5:25880353-25880372 MsG0580025881.01.T01:CDS 55.0%
GGGCCTGCAACAATATGGGT+TGG - Chr5:25879202-25879221 MsG0580025881.01.T01:CDS 55.0%
TGGCTGTGGAAGTGAGCTTG+GGG - Chr5:25879233-25879252 MsG0580025881.01.T01:intron 55.0%
! AACCAAAAGCAGGTGCCGCA+GGG - Chr5:25880399-25880418 MsG0580025881.01.T01:CDS 55.0%
GTTGCCTGCTACAGTCCCTG+CGG + Chr5:25880417-25880436 None:intergenic 60.0%
! CAACCAAAAGCAGGTGCCGC+AGG - Chr5:25880398-25880417 MsG0580025881.01.T01:CDS 60.0%
!! GCGGCACCTGCTTTTGGTTG+AGG + Chr5:25880398-25880417 None:intergenic 60.0%
CGAGGTTGAGGAGGATCGGG+AGG - Chr5:25880357-25880376 MsG0580025881.01.T01:CDS 65.0%
! GTCCCTGCGGCACCTGCTTT+TGG + Chr5:25880404-25880423 None:intergenic 65.0%
! GGTGCCGCAGGGACTGTAGC+AGG - Chr5:25880410-25880429 MsG0580025881.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 25878364 25880451 25878364 ID=MsG0580025881.01;Name=MsG0580025881.01
Chr5 mRNA 25878364 25880451 25878364 ID=MsG0580025881.01.T01;Parent=MsG0580025881.01;Name=MsG0580025881.01.T01;_AED=0.37;_eAED=0.54;_QI=0|0|0|1|1|1|5|0|306
Chr5 exon 25880244 25880451 25880244 ID=MsG0580025881.01.T01:exon:5119;Parent=MsG0580025881.01.T01
Chr5 exon 25879733 25879851 25879733 ID=MsG0580025881.01.T01:exon:5118;Parent=MsG0580025881.01.T01
Chr5 exon 25879385 25879658 25879385 ID=MsG0580025881.01.T01:exon:5117;Parent=MsG0580025881.01.T01
Chr5 exon 25879163 25879249 25879163 ID=MsG0580025881.01.T01:exon:5116;Parent=MsG0580025881.01.T01
Chr5 exon 25878364 25878596 25878364 ID=MsG0580025881.01.T01:exon:5115;Parent=MsG0580025881.01.T01
Chr5 CDS 25880244 25880451 25880244 ID=MsG0580025881.01.T01:cds;Parent=MsG0580025881.01.T01
Chr5 CDS 25879733 25879851 25879733 ID=MsG0580025881.01.T01:cds;Parent=MsG0580025881.01.T01
Chr5 CDS 25879385 25879658 25879385 ID=MsG0580025881.01.T01:cds;Parent=MsG0580025881.01.T01
Chr5 CDS 25879163 25879249 25879163 ID=MsG0580025881.01.T01:cds;Parent=MsG0580025881.01.T01
Chr5 CDS 25878364 25878596 25878364 ID=MsG0580025881.01.T01:cds;Parent=MsG0580025881.01.T01
Gene Sequence

>MsG0580025881.01.T01

ATGTTTGGTGTTAATACTTTAGAAATAAATTATTCCAATATAAAATCAGTTGATGAAGGTGCTGAAGACACAGAAGAAGGTGATGATGAAGAGAGTGATGAATTGAGGGATGAGAGTTCATACGAGGATGAAATAACAAAGCAATCTCAAAGAAAGGGAAAAAGAAAAGTGGGTATGCATTTTGACACTACCCAACCAAAATTCTCAGGTAGTAATAAAATAGCCATGGTGAAGGCGGCTAGAAAATACCCAACATCTGGAACTGTTAATGAGAATCCCTTTTTTGAAATTACATTGTCACATCATAACGCCAATGGCTATAATTTGTGGTTACCAAGTAAGTTTTCAAGAGAACACTTGATGGATTATAAAGGGCCTGCAACAATATGGGTTGGTAAGGAAATGGCTGTGGAAGTGAGCTTGGGGTTTTATAATACCTGCCAAAGATCTATTATGAGTAGTGGTTGGAAAATATTCAGGGAAAAATATAATTTGCAAGTTGACGATCATTGTAAATTTGAGATGATTCATCGGAGACCTGCATCATTCACTGTTACTATTACTCGTGGCAGAAATGGACCAAGCCCTAAGAAATTACCAGGGGTCTCTTATGGTAATAATCTTGTAAAGAAGAAAGACATTGGGGAAACTTCTTCTAGGAGCCGTCCAAAAGAAAATTTTCTTGAGGACATGTCTCAGAATCAGAATCAGGGTAACGTTCAGGGTGATGAGAAGAAGAAAGAAAAATCGCCTATGACATGGCACAGAATCACATGTGATGGTTCTGTCAAAAAGAAGGATTCCAAGGTAACGGTGTACAATCAGACGAGGTTGAGGAGGATCGGGAGGACATACTACTTTGGTCCTCAACCAAAAGCAGGTGCCGCAGGGACTGTAGCAGGCAACCCGATTGACTTATGA
Protein sequence

>MsG0580025881.01.T01

MFGVNTLEINYSNIKSVDEGAEDTEEGDDEESDELRDESSYEDEITKQSQRKGKRKVGMHFDTTQPKFSGSNKIAMVKAARKYPTSGTVNENPFFEITLSHHNANGYNLWLPSKFSREHLMDYKGPATIWVGKEMAVEVSLGFYNTCQRSIMSSGWKIFREKYNLQVDDHCKFEMIHRRPASFTVTITRGRNGPSPKKLPGVSYGNNLVKKKDIGETSSRSRPKENFLEDMSQNQNQGNVQGDEKKKEKSPMTWHRITCDGSVKKKDSKVTVYNQTRLRRIGRTYYFGPQPKAGAAGTVAGNPIDL*