Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026079.01.T01 | PNX78669.1 | 98.925 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 3.18E-57 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026079.01.T01 | Q8GW32 | 83.784 | 74 | 12 | 0 | 19 | 92 | 19 | 92 | 3.20E-38 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026079.01.T01 | A0A2K3LJK3 | 98.925 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 1.52e-57 | 181 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580026079.01.T01 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026079.01.T01 | MTR_5g039180 | 98.925 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 1.01e-60 | 180 |
| MsG0580026079.01.T01 | MTR_7g010120 | 72.826 | 92 | 24 | 1 | 1 | 92 | 1 | 91 | 1.05e-40 | 129 |
| MsG0580026079.01.T01 | MTR_6g011670 | 69.737 | 76 | 23 | 0 | 17 | 92 | 15 | 90 | 4.85e-34 | 112 |
| MsG0580026079.01.T01 | MTR_3g438070 | 74.026 | 77 | 18 | 1 | 17 | 93 | 17 | 91 | 5.01e-34 | 112 |
| MsG0580026079.01.T01 | MTR_7g059260 | 45.946 | 74 | 40 | 0 | 8 | 81 | 3 | 76 | 1.30e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026079.01.T01 | AT1G26945 | 83.784 | 74 | 12 | 0 | 19 | 92 | 19 | 92 | 3.26e-39 | 125 |
| MsG0580026079.01.T01 | AT3G28857 | 64.894 | 94 | 30 | 2 | 1 | 93 | 1 | 92 | 3.87e-36 | 118 |
| MsG0580026079.01.T01 | AT3G47710 | 69.149 | 94 | 25 | 3 | 1 | 93 | 1 | 91 | 3.62e-35 | 115 |
| MsG0580026079.01.T01 | AT5G15160 | 73.333 | 75 | 19 | 1 | 19 | 92 | 18 | 92 | 3.39e-32 | 108 |
| MsG0580026079.01.T01 | AT1G74500 | 72.727 | 77 | 18 | 2 | 17 | 92 | 17 | 91 | 3.07e-31 | 105 |
| MsG0580026079.01.T01 | AT5G39860 | 65.385 | 78 | 26 | 1 | 17 | 93 | 15 | 92 | 1.80e-30 | 103 |
Find 48 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGATAATAAAATTAAATT+TGG | 0.194346 | 5:+30294712 | None:intergenic |
| GCTAGACATTGTTATAATTA+AGG | 0.199410 | 5:+30294468 | None:intergenic |
| TATCTGTGTAGGGTTTAATT+TGG | 0.245749 | 5:-30293657 | MsG0580026079.01.T01:three_prime_UTR |
| TGACCGATTGTCACAACTTT+TGG | 0.275530 | 5:-30293828 | MsG0580026079.01.T01:CDS |
| GAGCGGCTAGCGCGAAGTTC+GGG | 0.276939 | 5:+30294355 | None:intergenic |
| GTGCTCAAGCAGCTATTATT+AGG | 0.330670 | 5:-30293787 | MsG0580026079.01.T01:CDS |
| GGAGCGGCTAGCGCGAAGTT+CGG | 0.362273 | 5:+30294354 | None:intergenic |
| GAAGTTATGTGAAATGAGTA+TGG | 0.366690 | 5:+30294617 | None:intergenic |
| GTTATTCTCTTATCTGTGTA+GGG | 0.379197 | 5:-30293667 | MsG0580026079.01.T01:three_prime_UTR |
| CTTATAGGTTTCAGCTACTA+AGG | 0.382941 | 5:-30293909 | MsG0580026079.01.T01:intron |
| TGTTATTCTCTTATCTGTGT+AGG | 0.387894 | 5:-30293668 | MsG0580026079.01.T01:three_prime_UTR |
| AGAGGAACCACCGGATTGTC+TGG | 0.389390 | 5:+30294432 | None:intergenic |
| GGGGATAAGTTGTTGTAACT+TGG | 0.418381 | 5:+30294375 | None:intergenic |
| GGAGGAGAGTGAAGAGGAGT+GGG | 0.429020 | 5:+30294738 | None:intergenic |
| GGTTTGTAGACCAAGGCAAA+AGG | 0.461588 | 5:-30293636 | MsG0580026079.01.T01:three_prime_UTR |
| AGAGAGGAAATGAGGTTGGT+TGG | 0.463003 | 5:+30294565 | None:intergenic |
| AAATAAACAAAGAGAAAGAG+AGG | 0.468720 | 5:+30294549 | None:intergenic |
| GATAATAAAATTAAATTTGG+AGG | 0.476768 | 5:+30294715 | None:intergenic |
| AGAAAGAGAGGAAATGAGGT+TGG | 0.479603 | 5:+30294561 | None:intergenic |
| GGTTGGTTGGTTGTCAGAGA+AGG | 0.483101 | 5:+30294578 | None:intergenic |
| ATAATAAAATTAAATTTGGA+GGG | 0.484167 | 5:+30294716 | None:intergenic |
| AAAGAGAAAGAGAGGAAATG+AGG | 0.488212 | 5:+30294557 | None:intergenic |
| AAGAGAGAAATAAATAAAAG+TGG | 0.494133 | 5:+30294661 | None:intergenic |
| AATAGCTGCTTGAGCACTGT+TGG | 0.496664 | 5:+30293793 | None:intergenic |
| TGTTATAATTAAGGAGGTAG+TGG | 0.505098 | 5:+30294477 | None:intergenic |
| TAAAATTAAATTTGGAGGGG+AGG | 0.505190 | 5:+30294720 | None:intergenic |
| TGTGAAATGAGTATGGAGTA+AGG | 0.505231 | 5:+30294624 | None:intergenic |
| AAGCCAAAAGTTGTGACAAT+CGG | 0.524888 | 5:+30293825 | None:intergenic |
| GTTTGTAGACCAAGGCAAAA+GGG | 0.528021 | 5:-30293635 | MsG0580026079.01.T01:three_prime_UTR |
| AAAATTAAACTAATTAATCA+AGG | 0.530033 | 5:+30293587 | None:intergenic |
| AGATCTCGTTCCAGACAATC+CGG | 0.532260 | 5:-30294442 | MsG0580026079.01.T01:CDS |
| GGGAGGAGAGTGAAGAGGAG+TGG | 0.537209 | 5:+30294737 | None:intergenic |
| TGGAGGGGAGGAGAGTGAAG+AGG | 0.555226 | 5:+30294732 | None:intergenic |
| GTGAAATGAGTATGGAGTAA+GGG | 0.559416 | 5:+30294625 | None:intergenic |
| AATTGAAGAGGACAAGTGCA+TGG | 0.569802 | 5:+30294772 | None:intergenic |
| GTTGAATTGTTGAATTGAAG+AGG | 0.578890 | 5:+30294760 | None:intergenic |
| TTAATTTGGTTTGTAGACCA+AGG | 0.581266 | 5:-30293643 | MsG0580026079.01.T01:three_prime_UTR |
| TAATAAAATTAAATTTGGAG+GGG | 0.593264 | 5:+30294717 | None:intergenic |
| AAATAAATACCTTGTTGGAG+CGG | 0.605762 | 5:+30294338 | None:intergenic |
| ATTGAAGAGGACAAGTGCAT+GGG | 0.605864 | 5:+30294773 | None:intergenic |
| AGACATTGTTATAATTAAGG+AGG | 0.612299 | 5:+30294471 | None:intergenic |
| TCGCGCTAGCCGCTCCAACA+AGG | 0.613710 | 5:-30294347 | MsG0580026079.01.T01:intron |
| AGTGATCTCAGAGGAACCAC+CGG | 0.628272 | 5:+30294423 | None:intergenic |
| AGCGGCTAGCGCGAAGTTCG+GGG | 0.650397 | 5:+30294356 | None:intergenic |
| AATTAATCAAGGACTAACAA+AGG | 0.658512 | 5:+30293598 | None:intergenic |
| AATAACAATAAAGTGAGACA+AGG | 0.708908 | 5:+30293684 | None:intergenic |
| TCTCGTTCCAGACAATCCGG+TGG | 0.728257 | 5:-30294439 | MsG0580026079.01.T01:CDS |
| TTGAGCATCAGTGATCTCAG+AGG | 0.728981 | 5:+30294414 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTAAACTAATTAATCA+AGG | + | Chr5:30294680-30294699 | None:intergenic | 10.0% |
| !! | ATAATAAAATTAAATTTGGA+GGG | + | Chr5:30293551-30293570 | None:intergenic | 10.0% |
| !! | GATAATAAAATTAAATTTGG+AGG | + | Chr5:30293552-30293571 | None:intergenic | 15.0% |
| !! | TAATAAAATTAAATTTGGAG+GGG | + | Chr5:30293550-30293569 | None:intergenic | 15.0% |
| !!! | ATTTTTTTTTATGGTCTTAT+AGG | - | Chr5:30294340-30294359 | MsG0580026079.01.T01:intron | 15.0% |
| !! | AAGAGAGAAATAAATAAAAG+TGG | + | Chr5:30293606-30293625 | None:intergenic | 20.0% |
| !! | TAGAAAAATAAATACCTTGT+TGG | + | Chr5:30293934-30293953 | None:intergenic | 20.0% |
| ! | AAATAAACAAAGAGAAAGAG+AGG | + | Chr5:30293718-30293737 | None:intergenic | 25.0% |
| ! | AATAACAATAAAGTGAGACA+AGG | + | Chr5:30294583-30294602 | None:intergenic | 25.0% |
| ! | AATTAATCAAGGACTAACAA+AGG | + | Chr5:30294669-30294688 | None:intergenic | 25.0% |
| ! | AGACATTGTTATAATTAAGG+AGG | + | Chr5:30293796-30293815 | None:intergenic | 25.0% |
| ! | GCTAGACATTGTTATAATTA+AGG | + | Chr5:30293799-30293818 | None:intergenic | 25.0% |
| !!! | TGTTTTTTGTAATGTTGCTA+AGG | + | Chr5:30293578-30293597 | None:intergenic | 25.0% |
| AAATAAATACCTTGTTGGAG+CGG | + | Chr5:30293929-30293948 | None:intergenic | 30.0% | |
| ACAATACAATACACAAGTTG+TGG | + | Chr5:30294191-30294210 | None:intergenic | 30.0% | |
| CATAAAAAACTTGCATAGAG+AGG | - | Chr5:30294400-30294419 | MsG0580026079.01.T01:CDS | 30.0% | |
| GAAGTTATGTGAAATGAGTA+TGG | + | Chr5:30293650-30293669 | None:intergenic | 30.0% | |
| GTTATTCTCTTATCTGTGTA+GGG | - | Chr5:30294597-30294616 | MsG0580026079.01.T01:five_prime_UTR | 30.0% | |
| GTTGAATTGTTGAATTGAAG+AGG | + | Chr5:30293507-30293526 | None:intergenic | 30.0% | |
| TAAAATTAAATTTGGAGGGG+AGG | + | Chr5:30293547-30293566 | None:intergenic | 30.0% | |
| TATCTGTGTAGGGTTTAATT+TGG | - | Chr5:30294607-30294626 | MsG0580026079.01.T01:five_prime_UTR | 30.0% | |
| TGTTATAATTAAGGAGGTAG+TGG | + | Chr5:30293790-30293809 | None:intergenic | 30.0% | |
| TGTTATTCTCTTATCTGTGT+AGG | - | Chr5:30294596-30294615 | MsG0580026079.01.T01:five_prime_UTR | 30.0% | |
| !! | TTAATTTGGTTTGTAGACCA+AGG | - | Chr5:30294621-30294640 | MsG0580026079.01.T01:five_prime_UTR | 30.0% |
| AAAGAGAAAGAGAGGAAATG+AGG | + | Chr5:30293710-30293729 | None:intergenic | 35.0% | |
| AAGCCAAAAGTTGTGACAAT+CGG | + | Chr5:30294442-30294461 | None:intergenic | 35.0% | |
| CTTATAGGTTTCAGCTACTA+AGG | - | Chr5:30294355-30294374 | MsG0580026079.01.T01:CDS | 35.0% | |
| GATACAATGCACATATTGTG+TGG | - | Chr5:30294108-30294127 | MsG0580026079.01.T01:intron | 35.0% | |
| GTGAAATGAGTATGGAGTAA+GGG | + | Chr5:30293642-30293661 | None:intergenic | 35.0% | |
| TGTGAAATGAGTATGGAGTA+AGG | + | Chr5:30293643-30293662 | None:intergenic | 35.0% | |
| ! | ATTTGTGAGAGTGTGTATGA+AGG | + | Chr5:30294053-30294072 | None:intergenic | 35.0% |
| !! | TGTGTGGACATTGTTAGTTT+TGG | - | Chr5:30294124-30294143 | MsG0580026079.01.T01:intron | 35.0% |
| AGAAAGAGAGGAAATGAGGT+TGG | + | Chr5:30293706-30293725 | None:intergenic | 40.0% | |
| CAAGTTGTGGCAAAGAATTG+AGG | + | Chr5:30294178-30294197 | None:intergenic | 40.0% | |
| GGGGATAAGTTGTTGTAACT+TGG | + | Chr5:30293892-30293911 | None:intergenic | 40.0% | |
| GTGCATGTGAATGATGACTT+GGG | + | Chr5:30294077-30294096 | None:intergenic | 40.0% | |
| GTGCTCAAGCAGCTATTATT+AGG | - | Chr5:30294477-30294496 | MsG0580026079.01.T01:five_prime_UTR | 40.0% | |
| GTTTGTAGACCAAGGCAAAA+GGG | - | Chr5:30294629-30294648 | MsG0580026079.01.T01:five_prime_UTR | 40.0% | |
| TGTGCATGTGAATGATGACT+TGG | + | Chr5:30294078-30294097 | None:intergenic | 40.0% | |
| ! | TATAGTAAGCCCTTTTGCCT+TGG | + | Chr5:30294641-30294660 | None:intergenic | 40.0% |
| ! | TGACCGATTGTCACAACTTT+TGG | - | Chr5:30294436-30294455 | MsG0580026079.01.T01:CDS | 40.0% |
| AGATCTCGTTCCAGACAATC+CGG | - | Chr5:30293822-30293841 | MsG0580026079.01.T01:CDS | 45.0% | |
| AGCTAGTTGCAACATGCAAG+TGG | - | Chr5:30294252-30294271 | MsG0580026079.01.T01:intron | 45.0% | |
| GCTAGTTGCAACATGCAAGT+GGG | - | Chr5:30294253-30294272 | MsG0580026079.01.T01:intron | 45.0% | |
| GGTTTGTAGACCAAGGCAAA+AGG | - | Chr5:30294628-30294647 | MsG0580026079.01.T01:five_prime_UTR | 45.0% | |
| TTGAGCATCAGTGATCTCAG+AGG | + | Chr5:30293853-30293872 | None:intergenic | 45.0% | |
| ! | AATAGCTGCTTGAGCACTGT+TGG | + | Chr5:30294474-30294493 | None:intergenic | 45.0% |
| ! | AGAGAGGAAATGAGGTTGGT+TGG | + | Chr5:30293702-30293721 | None:intergenic | 45.0% |
| !! | TATGATAATAAAATTAAATT+TGG | + | Chr5:30293555-30293574 | None:intergenic | 5.0% |
| !!! | TTAATTTGTATTTTTTTTTA+TGG | - | Chr5:30294331-30294350 | MsG0580026079.01.T01:intron | 5.0% |
| AGTGATCTCAGAGGAACCAC+CGG | + | Chr5:30293844-30293863 | None:intergenic | 50.0% | |
| AGTTGCAACATGCAAGTGGG+TGG | - | Chr5:30294256-30294275 | MsG0580026079.01.T01:intron | 50.0% | |
| ! | GGTTGGTTGGTTGTCAGAGA+AGG | + | Chr5:30293689-30293708 | None:intergenic | 50.0% |
| AGAGGAACCACCGGATTGTC+TGG | + | Chr5:30293835-30293854 | None:intergenic | 55.0% | |
| GGAGGAGAGTGAAGAGGAGT+GGG | + | Chr5:30293529-30293548 | None:intergenic | 55.0% | |
| TCTCGTTCCAGACAATCCGG+TGG | - | Chr5:30293825-30293844 | MsG0580026079.01.T01:CDS | 55.0% | |
| GGGAGGAGAGTGAAGAGGAG+TGG | + | Chr5:30293530-30293549 | None:intergenic | 60.0% | |
| TGGAGGGGAGGAGAGTGAAG+AGG | + | Chr5:30293535-30293554 | None:intergenic | 60.0% | |
| AGCGGCTAGCGCGAAGTTCG+GGG | + | Chr5:30293911-30293930 | None:intergenic | 65.0% | |
| GAGCGGCTAGCGCGAAGTTC+GGG | + | Chr5:30293912-30293931 | None:intergenic | 65.0% | |
| GGAGCGGCTAGCGCGAAGTT+CGG | + | Chr5:30293913-30293932 | None:intergenic | 65.0% | |
| TCGCGCTAGCCGCTCCAACA+AGG | - | Chr5:30293917-30293936 | MsG0580026079.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 30293494 | 30294792 | 30293494 | ID=MsG0580026079.01;Name=MsG0580026079.01 |
| Chr5 | mRNA | 30293494 | 30294792 | 30293494 | ID=MsG0580026079.01.T01;Parent=MsG0580026079.01;Name=MsG0580026079.01.T01;_AED=0.29;_eAED=0.29;_QI=316|1|1|1|1|1|2|278|93 |
| Chr5 | exon | 30293494 | 30293924 | 30293494 | ID=MsG0580026079.01.T01:exon:7297;Parent=MsG0580026079.01.T01 |
| Chr5 | exon | 30294348 | 30294792 | 30294348 | ID=MsG0580026079.01.T01:exon:7296;Parent=MsG0580026079.01.T01 |
| Chr5 | five_prime_UTR | 30294477 | 30294792 | 30294477 | ID=MsG0580026079.01.T01:five_prime_utr;Parent=MsG0580026079.01.T01 |
| Chr5 | CDS | 30294348 | 30294476 | 30294348 | ID=MsG0580026079.01.T01:cds;Parent=MsG0580026079.01.T01 |
| Chr5 | CDS | 30293772 | 30293924 | 30293772 | ID=MsG0580026079.01.T01:cds;Parent=MsG0580026079.01.T01 |
| Chr5 | three_prime_UTR | 30293494 | 30293771 | 30293494 | ID=MsG0580026079.01.T01:three_prime_utr;Parent=MsG0580026079.01.T01 |
| Gene Sequence |
| Protein sequence |
