AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026193.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026193.01.T01 MTR_5g041420 87.850 214 22 3 1 211 1 213 1.29e-121 348
MsG0580026193.01.T01 MTR_5g041380 70.306 229 50 3 1 225 1 215 1.07e-97 289
MsG0580026193.01.T01 MTR_5g041530 67.290 214 62 2 1 211 1 209 1.07e-90 272
MsG0580026193.01.T01 MTR_5g041400 67.111 225 48 5 1 221 1 203 3.36e-88 265
MsG0580026193.01.T01 MTR_4g082060 42.510 247 77 8 2 216 4 217 1.25e-47 163
MsG0580026193.01.T01 MTR_7g010950 41.600 250 92 9 4 221 6 233 9.38e-45 156
MsG0580026193.01.T01 MTR_6g012450 39.167 240 112 8 6 219 8 239 2.72e-40 144
MsG0580026193.01.T01 MTR_3g435480 37.179 234 116 7 7 215 9 236 5.75e-36 132
MsG0580026193.01.T01 MTR_7g086780 53.279 122 33 3 120 219 84 203 2.47e-35 130
MsG0580026193.01.T01 MTR_6g027460 55.670 97 40 1 97 190 53 149 9.18e-34 124
MsG0580026193.01.T01 MTR_8g044220 72.727 66 18 0 125 190 106 171 4.02e-33 124
MsG0580026193.01.T01 MTR_6g027450 53.125 96 42 1 98 190 47 142 7.46e-31 116
MsG0580026193.01.T01 MTR_2g016030 70.000 60 18 0 131 190 41 100 1.94e-30 110
MsG0580026193.01.T01 MTR_2g093220 63.636 66 23 1 126 190 28 93 3.31e-26 103
MsG0580026193.01.T01 MTR_3g091820 57.971 69 29 0 124 192 30 98 1.61e-25 101
MsG0580026193.01.T01 MTR_8g079060 63.934 61 22 0 129 189 21 81 2.99e-25 99.4
MsG0580026193.01.T01 MTR_8g015840 62.500 64 23 1 126 188 8 71 5.88e-25 98.2
MsG0580026193.01.T01 MTR_2g096740 55.714 70 31 0 121 190 46 115 1.04e-24 99.4
MsG0580026193.01.T01 MTR_2g096740 55.714 70 31 0 121 190 61 130 1.18e-24 99.4
MsG0580026193.01.T01 MTR_4g461080 61.538 65 24 1 126 189 17 81 2.07e-24 98.2
MsG0580026193.01.T01 MTR_4g022370 48.684 76 39 0 115 190 60 135 3.25e-24 99.0
MsG0580026193.01.T01 MTR_8g027295 61.017 59 23 0 132 190 19 77 3.28e-24 97.4
MsG0580026193.01.T01 MTR_3g090430 57.746 71 28 2 122 190 34 104 9.80e-24 97.4
MsG0580026193.01.T01 MTR_8g479350 67.857 56 18 0 135 190 66 121 1.11e-23 97.4
MsG0580026193.01.T01 MTR_4g109980 67.925 53 17 0 135 187 38 90 1.63e-23 96.7
MsG0580026193.01.T01 MTR_1g077600 59.375 64 25 1 127 189 32 95 1.92e-23 95.5
MsG0580026193.01.T01 MTR_1g077600 59.375 64 25 1 127 189 32 95 2.07e-23 95.9
MsG0580026193.01.T01 MTR_4g089095 67.925 53 17 0 139 191 52 104 2.79e-23 95.5
MsG0580026193.01.T01 MTR_7g024670 70.588 51 15 0 139 189 79 129 3.07e-23 96.7
MsG0580026193.01.T01 MTR_1g056810 63.333 60 21 1 132 190 6 65 3.17e-23 95.1
MsG0580026193.01.T01 MTR_4g089095 67.925 53 17 0 139 191 18 70 3.87e-23 94.4
MsG0580026193.01.T01 MTR_8g068210 54.167 72 33 0 119 190 14 85 4.56e-23 95.5
MsG0580026193.01.T01 MTR_4g088580 69.231 52 16 0 139 190 57 108 8.64e-23 95.5
MsG0580026193.01.T01 MTR_4g063780 61.404 57 22 0 133 189 71 127 1.00e-22 94.7
MsG0580026193.01.T01 MTR_2g059540 68.000 50 16 0 136 185 30 79 1.02e-22 92.0
MsG0580026193.01.T01 MTR_2g013370 61.404 57 22 0 135 191 44 100 1.60e-22 93.2
MsG0580026193.01.T01 MTR_5g031440 65.455 55 19 0 133 187 39 93 1.89e-22 94.0
MsG0580026193.01.T01 MTR_7g059400 63.462 52 19 0 139 190 78 129 2.72e-21 90.9
MsG0580026193.01.T01 MTR_2g014170 66.000 50 17 0 136 185 44 93 4.33e-21 89.7
MsG0580026193.01.T01 MTR_2g014060 68.627 51 16 0 139 189 27 77 5.80e-21 89.7
MsG0580026193.01.T01 MTR_3g077750 59.677 62 24 1 127 187 50 111 7.92e-21 89.4
MsG0580026193.01.T02 MTR_5g041420 92.135 89 6 1 1 88 125 213 4.91e-57 179
MsG0580026193.01.T02 MTR_5g041400 77.551 98 19 1 1 98 109 203 5.08e-52 167
MsG0580026193.01.T02 MTR_5g041380 75.490 102 22 1 1 102 117 215 4.71e-51 165
MsG0580026193.01.T02 MTR_5g041530 71.591 88 20 1 1 88 127 209 8.24e-42 141
MsG0580026193.01.T02 MTR_7g010950 56.034 116 27 2 2 98 123 233 2.27e-38 134
MsG0580026193.01.T02 MTR_7g086780 53.448 116 32 2 3 96 88 203 1.23e-34 123
MsG0580026193.01.T02 MTR_4g082060 51.327 113 31 2 1 93 109 217 1.40e-34 123
MsG0580026193.01.T02 MTR_6g012450 53.097 113 32 3 2 93 124 236 9.16e-34 121
MsG0580026193.01.T02 MTR_8g044220 72.059 68 19 0 2 69 106 173 1.92e-32 117
MsG0580026193.01.T02 MTR_6g027460 72.308 65 18 0 3 67 85 149 7.07e-32 115
MsG0580026193.01.T02 MTR_3g435480 61.446 83 27 1 2 84 124 201 1.16e-31 115
MsG0580026193.01.T02 MTR_2g016030 70.000 60 18 0 8 67 41 100 3.72e-30 105
MsG0580026193.01.T02 MTR_6g027450 67.692 65 21 0 3 67 78 142 4.01e-29 107
MsG0580026193.01.T02 MTR_2g093220 63.636 66 23 1 3 67 28 93 1.21e-25 97.4
MsG0580026193.01.T02 MTR_8g079060 63.934 61 22 0 6 66 21 81 3.04e-25 94.7
MsG0580026193.01.T02 MTR_3g091820 57.971 69 29 0 1 69 30 98 3.28e-25 96.3
MsG0580026193.01.T02 MTR_8g015840 62.500 64 23 1 3 65 8 71 1.48e-24 92.8
MsG0580026193.01.T02 MTR_8g027295 61.017 59 23 0 9 67 19 77 5.49e-24 92.4
MsG0580026193.01.T02 MTR_4g461080 61.538 65 24 1 3 66 17 81 5.89e-24 92.4
MsG0580026193.01.T02 MTR_2g096740 58.462 65 27 0 3 67 51 115 7.89e-24 92.4
MsG0580026193.01.T02 MTR_2g096740 58.462 65 27 0 3 67 66 130 9.86e-24 92.4
MsG0580026193.01.T02 MTR_4g022370 60.345 58 23 0 10 67 78 135 2.31e-23 92.4
MsG0580026193.01.T02 MTR_1g077600 59.375 64 25 1 4 66 32 95 4.34e-23 90.1
MsG0580026193.01.T02 MTR_1g077600 59.375 64 25 1 4 66 32 95 4.67e-23 90.5
MsG0580026193.01.T02 MTR_8g479350 67.857 56 18 0 12 67 66 121 5.55e-23 90.9
MsG0580026193.01.T02 MTR_4g109980 67.925 53 17 0 12 64 38 90 7.20e-23 90.5
MsG0580026193.01.T02 MTR_4g089095 67.925 53 17 0 16 68 52 104 7.68e-23 90.1
MsG0580026193.01.T02 MTR_4g089095 67.925 53 17 0 16 68 18 70 9.83e-23 89.0
MsG0580026193.01.T02 MTR_7g024670 70.588 51 15 0 16 66 79 129 1.33e-22 90.1
MsG0580026193.01.T02 MTR_1g056810 63.333 60 21 1 9 67 6 65 2.06e-22 88.6
MsG0580026193.01.T02 MTR_3g090430 63.158 57 20 1 12 67 48 104 2.73e-22 89.0
MsG0580026193.01.T02 MTR_8g068210 67.925 53 17 0 13 65 31 83 3.20e-22 89.0
MsG0580026193.01.T02 MTR_2g013370 61.404 57 22 0 12 68 44 100 3.24e-22 87.8
MsG0580026193.01.T02 MTR_4g088580 69.231 52 16 0 16 67 57 108 3.28e-22 89.4
MsG0580026193.01.T02 MTR_2g059540 68.000 50 16 0 13 62 30 79 3.42e-22 86.3
MsG0580026193.01.T02 MTR_4g063780 61.404 57 22 0 10 66 71 127 4.13e-22 88.6
MsG0580026193.01.T02 MTR_5g031440 65.455 55 19 0 10 64 39 93 8.24e-22 87.8
MsG0580026193.01.T02 MTR_2g014170 66.000 50 17 0 13 62 44 93 7.60e-21 84.7
MsG0580026193.01.T02 MTR_7g059400 63.462 52 19 0 16 67 78 129 1.01e-20 85.1
MsG0580026193.01.T02 MTR_3g077750 64.815 54 19 0 11 64 58 111 1.85e-20 84.0
MsG0580026193.01.T02 MTR_2g014060 70.000 50 15 0 16 65 27 76 3.12e-20 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026193.01.T01 AT5G39660 36.585 246 123 6 2 221 1 239 2.31e-36 133
MsG0580026193.01.T01 AT5G39660 36.585 246 123 6 2 221 1 239 2.31e-36 133
MsG0580026193.01.T01 AT5G39660 36.585 246 123 6 2 221 1 239 2.31e-36 133
MsG0580026193.01.T01 AT5G62430 60.204 98 32 3 122 218 38 129 1.77e-34 125
MsG0580026193.01.T01 AT3G47500 57.447 94 40 0 102 195 75 168 5.78e-33 124
MsG0580026193.01.T01 AT1G29160 65.217 69 21 1 122 190 50 115 1.14e-32 117
MsG0580026193.01.T01 AT1G69570 54.348 92 29 2 131 212 126 214 2.35e-32 121
MsG0580026193.01.T01 AT2G34140 46.018 113 51 3 85 190 2 111 1.19e-31 114
MsG0580026193.01.T01 AT1G26790 71.667 60 17 0 131 190 105 164 5.38e-31 117
MsG0580026193.01.T01 AT1G51700 60.000 65 25 1 125 188 20 84 1.24e-25 99.4
MsG0580026193.01.T01 AT5G60200 61.905 63 24 0 130 192 48 110 1.88e-25 100
MsG0580026193.01.T01 AT2G37590 50.000 74 37 0 117 190 69 142 6.33e-25 100
MsG0580026193.01.T01 AT4G24060 59.091 66 27 0 125 190 41 106 6.49e-25 100
MsG0580026193.01.T01 AT2G28810 44.318 88 47 1 101 188 43 128 1.68e-24 99.4
MsG0580026193.01.T01 AT2G28810 44.318 88 47 1 101 188 60 145 2.47e-24 99.0
MsG0580026193.01.T01 AT3G45610 60.317 63 25 0 130 192 33 95 4.45e-24 96.7
MsG0580026193.01.T01 AT3G55370 40.860 93 54 1 98 190 83 174 7.47e-24 98.2
MsG0580026193.01.T01 AT3G55370 41.053 95 55 1 98 192 38 131 8.79e-24 97.4
MsG0580026193.01.T01 AT5G60850 65.517 58 20 0 135 192 49 106 9.35e-24 97.1
MsG0580026193.01.T01 AT3G55370 41.053 95 55 1 98 192 38 131 1.18e-23 97.4
MsG0580026193.01.T01 AT3G50410 69.231 52 16 0 137 188 30 81 1.19e-23 95.5
MsG0580026193.01.T01 AT3G61850 60.656 61 24 0 132 192 57 117 1.46e-23 95.9
MsG0580026193.01.T01 AT3G61850 60.656 61 24 0 132 192 57 117 1.46e-23 95.9
MsG0580026193.01.T01 AT3G21270 70.588 51 15 0 139 189 31 81 1.76e-23 94.0
MsG0580026193.01.T01 AT3G61850 60.656 61 24 0 132 192 69 129 1.78e-23 95.9
MsG0580026193.01.T01 AT3G61850 60.656 61 24 0 132 192 57 117 1.99e-23 96.3
MsG0580026193.01.T01 AT3G61850 60.656 61 24 0 132 192 69 129 2.51e-23 96.3
MsG0580026193.01.T01 AT1G07640 68.627 51 16 0 139 189 23 73 2.72e-23 95.1
MsG0580026193.01.T01 AT1G07640 68.627 51 16 0 139 189 79 129 3.22e-23 95.9
MsG0580026193.01.T01 AT1G07640 68.627 51 16 0 139 189 87 137 3.91e-23 95.9
MsG0580026193.01.T01 AT5G66940 44.681 94 39 1 95 188 3 83 7.94e-23 92.8
MsG0580026193.01.T01 AT1G64620 56.250 64 27 1 122 185 35 97 1.99e-22 94.0
MsG0580026193.01.T01 AT5G65590 47.872 94 47 1 100 193 6 97 2.36e-22 93.6
MsG0580026193.01.T01 AT1G47655 48.718 78 37 1 108 185 3 77 2.48e-22 91.3
MsG0580026193.01.T01 AT3G52440 65.385 52 18 0 139 190 27 78 3.10e-22 91.7
MsG0580026193.01.T01 AT1G28310 67.308 52 17 0 139 190 29 80 4.11e-22 92.8
MsG0580026193.01.T01 AT2G28510 65.385 52 18 0 139 190 50 101 4.28e-22 92.0
MsG0580026193.01.T01 AT3G52440 61.404 57 21 1 135 190 41 97 4.42e-22 91.7
MsG0580026193.01.T01 AT1G28310 67.308 52 17 0 139 190 53 104 5.56e-22 92.8
MsG0580026193.01.T01 AT4G38000 58.209 67 26 2 125 190 30 95 8.80e-22 90.5
MsG0580026193.01.T01 AT4G00940 57.627 59 25 0 132 190 63 121 1.45e-21 90.9
MsG0580026193.01.T01 AT4G00940 57.627 59 25 0 132 190 63 121 1.45e-21 90.9
MsG0580026193.01.T01 AT4G00940 57.627 59 25 0 132 190 63 121 1.45e-21 90.9
MsG0580026193.01.T01 AT1G21340 66.000 50 17 0 139 188 40 89 1.58e-21 90.1
MsG0580026193.01.T01 AT5G02460 58.621 58 24 0 133 190 91 148 2.58e-21 91.7
MsG0580026193.01.T01 AT2G46590 60.377 53 21 0 132 184 63 115 5.83e-21 90.1
MsG0580026193.01.T01 AT2G46590 60.377 53 21 0 132 184 75 127 6.86e-21 90.1
MsG0580026193.01.T01 AT5G62940 41.228 114 60 3 76 187 15 123 1.23e-20 89.4
MsG0580026193.01.T01 AT4G21050 54.688 64 29 0 125 188 12 75 4.93e-20 85.1
MsG0580026193.01.T01 AT4G21040 63.462 52 19 0 139 190 27 78 2.35e-18 81.3
MsG0580026193.01.T01 AT4G21080 63.462 52 19 0 139 190 27 78 1.00e-17 79.7
MsG0580026193.01.T02 AT5G39660 57.009 107 29 2 2 91 126 232 1.16e-34 124
MsG0580026193.01.T02 AT5G39660 57.009 107 29 2 2 91 126 232 1.16e-34 124
MsG0580026193.01.T02 AT5G39660 57.009 107 29 2 2 91 126 232 1.16e-34 124
MsG0580026193.01.T02 AT5G62430 60.215 93 31 2 3 95 43 129 4.31e-34 119
MsG0580026193.01.T02 AT1G29160 66.667 66 22 0 2 67 50 115 5.52e-32 110
MsG0580026193.01.T02 AT3G47500 70.588 68 20 0 5 72 101 168 8.71e-32 115
MsG0580026193.01.T02 AT1G69570 52.525 99 40 2 8 99 126 224 1.29e-31 114
MsG0580026193.01.T02 AT1G26790 51.546 97 38 2 8 95 105 201 5.75e-31 112
MsG0580026193.01.T02 AT2G34140 66.129 62 21 0 6 67 50 111 4.35e-30 105
MsG0580026193.01.T02 AT1G51700 60.000 65 25 1 2 65 20 84 2.47e-25 94.4
MsG0580026193.01.T02 AT5G60200 61.905 63 24 0 7 69 48 110 6.97e-25 94.7
MsG0580026193.01.T02 AT4G24060 59.091 66 27 0 2 67 41 106 9.65e-25 95.9
MsG0580026193.01.T02 AT3G45610 60.317 63 25 0 7 69 33 95 8.21e-24 91.7
MsG0580026193.01.T02 AT2G37590 54.545 66 30 0 2 67 77 142 1.71e-23 92.0
MsG0580026193.01.T02 AT3G50410 67.925 53 17 0 14 66 30 82 2.69e-23 90.5
MsG0580026193.01.T02 AT3G61850 55.882 68 30 0 2 69 50 117 3.01e-23 90.9
MsG0580026193.01.T02 AT3G61850 55.882 68 30 0 2 69 50 117 3.01e-23 90.9
MsG0580026193.01.T02 AT3G61850 55.882 68 30 0 2 69 62 129 3.46e-23 90.9
MsG0580026193.01.T02 AT3G21270 70.588 51 15 0 16 66 31 81 3.78e-23 89.0
MsG0580026193.01.T02 AT5G60850 65.517 58 20 0 12 69 49 106 4.52e-23 90.9
MsG0580026193.01.T02 AT3G61850 55.882 68 30 0 2 69 50 117 4.59e-23 90.9
MsG0580026193.01.T02 AT3G61850 55.882 68 30 0 2 69 62 129 5.40e-23 90.9
MsG0580026193.01.T02 AT3G55370 60.000 60 24 0 10 69 72 131 5.62e-23 90.9
MsG0580026193.01.T02 AT3G55370 60.345 58 23 0 10 67 117 174 5.71e-23 91.3
MsG0580026193.01.T02 AT3G55370 60.000 60 24 0 10 69 72 131 6.52e-23 90.9
MsG0580026193.01.T02 AT5G66940 60.000 60 24 0 7 66 25 84 1.00e-22 88.2
MsG0580026193.01.T02 AT2G28810 57.377 61 26 0 5 65 68 128 1.63e-22 89.4
MsG0580026193.01.T02 AT1G07640 68.627 51 16 0 16 66 23 73 1.76e-22 88.6
MsG0580026193.01.T02 AT2G28810 57.377 61 26 0 5 65 85 145 2.22e-22 89.4
MsG0580026193.01.T02 AT1G07640 68.627 51 16 0 16 66 79 129 2.45e-22 89.0
MsG0580026193.01.T02 AT1G07640 68.627 51 16 0 16 66 87 137 2.96e-22 89.0
MsG0580026193.01.T02 AT1G47655 60.714 56 22 0 7 62 22 77 6.94e-22 85.9
MsG0580026193.01.T02 AT1G28310 64.815 54 19 0 14 67 27 80 8.42e-22 87.4
MsG0580026193.01.T02 AT1G28310 67.308 52 17 0 16 67 53 104 1.08e-21 87.4
MsG0580026193.01.T02 AT1G64620 62.500 56 20 1 7 62 43 97 1.08e-21 87.8
MsG0580026193.01.T02 AT5G65590 62.712 59 22 0 12 70 39 97 1.29e-21 87.0
MsG0580026193.01.T02 AT3G52440 48.864 88 40 2 12 98 22 105 1.37e-21 85.9
MsG0580026193.01.T02 AT4G00940 57.627 59 25 0 9 67 63 121 1.78e-21 86.3
MsG0580026193.01.T02 AT4G00940 57.627 59 25 0 9 67 63 121 1.78e-21 86.3
MsG0580026193.01.T02 AT4G00940 57.627 59 25 0 9 67 63 121 1.78e-21 86.3
MsG0580026193.01.T02 AT4G38000 58.209 67 26 2 2 67 30 95 1.89e-21 85.5
MsG0580026193.01.T02 AT3G52440 48.864 88 40 2 12 98 41 124 2.10e-21 85.5
MsG0580026193.01.T02 AT2G28510 65.385 52 18 0 16 67 50 101 2.20e-21 85.9
MsG0580026193.01.T02 AT5G02460 58.621 58 24 0 10 67 91 148 4.88e-21 86.3
MsG0580026193.01.T02 AT1G21340 66.000 50 17 0 16 65 40 89 7.83e-21 84.0
MsG0580026193.01.T02 AT2G46590 60.377 53 21 0 9 61 63 115 1.73e-20 84.3
MsG0580026193.01.T02 AT2G46590 60.377 53 21 0 9 61 75 127 1.97e-20 84.3
MsG0580026193.01.T02 AT5G62940 69.388 49 15 0 16 64 75 123 3.47e-20 83.6
MsG0580026193.01.T02 AT4G21050 54.688 64 29 0 2 65 12 75 1.27e-19 80.1
MsG0580026193.01.T02 AT4G21040 63.462 52 19 0 16 67 27 78 2.24e-18 77.0
MsG0580026193.01.T02 AT4G21080 63.462 52 19 0 16 67 27 78 1.36e-17 75.5

Find 27 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACTTGGTCTGGTTTCTT+TGG 0.248772 5:-32082228 None:intergenic
TGTGAAGTTGAAGACATAAT+AGG 0.290971 5:-32082483 None:intergenic
CTCCAGAAGTCCAGTATCTT+TGG 0.293736 5:-32082341 None:intergenic
TGCCAAAGATACTGGACTTC+TGG 0.300675 5:+32082339 MsG0580026193.01.T01:CDS
ATGAGTACTGTCTTGACATT+TGG 0.342898 5:+32082450 MsG0580026193.01.T01:CDS
ACATGATGCAAAGAGATATA+AGG 0.388068 5:+32081374 MsG0580026193.01.T01:CDS
ATTCTGAATCCTCTGTGGTT+TGG 0.395071 5:-32082089 None:intergenic
GGACATGGAACAACTTGGTC+TGG 0.406395 5:-32082237 None:intergenic
ACCACGAGAAATATGCTTAT+AGG 0.437951 5:+32082366 MsG0580026193.01.T01:CDS
CTCAATAAGGCTTGAAGATG+AGG 0.475849 5:-32082115 None:intergenic
GTTGAAGAATTCACTTCAAG+TGG 0.481761 5:-32081348 None:intergenic
AAATCTTCATGAGCAATAGT+AGG 0.488963 5:-32081285 None:intergenic
TACTGTCTTGACATTTGGAT+CGG 0.502009 5:+32082455 MsG0580026193.01.T01:CDS
AAAAGAACTCATCGAGGATG+AGG 0.512018 5:+32082059 MsG0580026193.01.T01:CDS
GACGTCTCTGTTTCAGATGA+GGG 0.514212 5:-32082150 None:intergenic
AGAAATATGCTTATAGGCGC+AGG 0.536173 5:+32082372 MsG0580026193.01.T01:CDS
AGACGTCTCTGTTTCAGATG+AGG 0.571871 5:-32082151 None:intergenic
ATCTTGGACATGGAACAACT+TGG 0.574876 5:-32082242 None:intergenic
GCCTATAAGCATATTTCTCG+TGG 0.581874 5:-32082367 None:intergenic
AATAGTAGGAGTAAATTCTG+AGG 0.595274 5:-32081271 None:intergenic
TCAATAAGGCTTGAAGATGA+GGG 0.621600 5:-32082114 None:intergenic
CAAAGATACTGGACTTCTGG+AGG 0.634659 5:+32082342 MsG0580026193.01.T01:CDS
ATGTCCAAGATGCAAAAGCA+CGG 0.662046 5:+32082254 MsG0580026193.01.T01:CDS
GGGAGATTCTGAATCCTCTG+TGG 0.678050 5:-32082094 None:intergenic
ATCAAGAAAAGAACTCATCG+AGG 0.704142 5:+32082053 MsG0580026193.01.T01:CDS
GGCTTCATTCCAAACCACAG+AGG 0.724092 5:+32082080 MsG0580026193.01.T01:CDS
ACTCATTTGATGATGATGTG+AGG 0.757465 5:-32082433 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATAAGAACAAATAAAT+CGG + Chr5:32081815-32081834 MsG0580026193.01.T01:intron 10.0%
!! AATACATGTAGTTATATTAT+CGG + Chr5:32081904-32081923 MsG0580026193.01.T01:intron 15.0%
!!! TATAAGCCAATAAAATTTTA+TGG + Chr5:32081983-32082002 MsG0580026193.01.T01:intron 15.0%
!!! TTCAAATTTTTCATCTTTTT+TGG + Chr5:32081473-32081492 MsG0580026193.01.T01:intron 15.0%
!!! AAATTTTATGGCTCATATTT+TGG + Chr5:32081995-32082014 MsG0580026193.01.T01:intron 20.0%
!!! TATGAGCCATAAAATTTTAT+TGG - Chr5:32081992-32082011 None:intergenic 20.0%
! AAACAACAGAAAATTCATGT+TGG - Chr5:32081793-32081812 None:intergenic 25.0%
! AACAGCTATATATCAATTTC+AGG + Chr5:32082026-32082045 MsG0580026193.01.T01:intron 25.0%
! TTCTGTTCGAAAAATATACA+CGG + Chr5:32081576-32081595 MsG0580026193.01.T01:intron 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + Chr5:32081198-32081217 MsG0580026193.01.T01:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + Chr5:32081199-32081218 MsG0580026193.01.T01:CDS 25.0%
!!! AGTTTGTTCATCAATTTTAG+TGG - Chr5:32082187-32082206 None:intergenic 25.0%
!!! TATATTTTGATCCTAACATG+AGG - Chr5:32081688-32081707 None:intergenic 25.0%
AAAAAGAGATTCTGTTCTTG+CGG - Chr5:32082401-32082420 None:intergenic 30.0%
AAAATATGTTGCTAGCTAGT+AGG + Chr5:32081445-32081464 MsG0580026193.01.T01:intron 30.0%
AAATCTTCATGAGCAATAGT+AGG - Chr5:32081288-32081307 None:intergenic 30.0%
AATAGTAACAAGAACCTTGA+TGG - Chr5:32081725-32081744 None:intergenic 30.0%
AATAGTAGGAGTAAATTCTG+AGG - Chr5:32081274-32081293 None:intergenic 30.0%
ACATGATGCAAAGAGATATA+AGG + Chr5:32081374-32081393 MsG0580026193.01.T01:CDS 30.0%
GCATCTCTCTTTATATTTGT+TGG - Chr5:32081757-32081776 None:intergenic 30.0%
GTCTTCAACTTCACAAAAAA+GGG + Chr5:32082491-32082510 MsG0580026193.01.T01:CDS 30.0%
TATATGAGAAACCTCATGTT+AGG + Chr5:32081674-32081693 MsG0580026193.01.T01:intron 30.0%
TGTCTTCAACTTCACAAAAA+AGG + Chr5:32082490-32082509 MsG0580026193.01.T01:CDS 30.0%
TGTGAAGTTGAAGACATAAT+AGG - Chr5:32082486-32082505 None:intergenic 30.0%
TTCTTCAAATAGAATCACCA+TGG - Chr5:32081411-32081430 None:intergenic 30.0%
! CTCTCTTTATATTTGTTGGT+AGG - Chr5:32081753-32081772 None:intergenic 30.0%
! GTTGATGAAATTGTTCATCA+AGG + Chr5:32081598-32081617 MsG0580026193.01.T01:intron 30.0%
! TACATGTATTGACATCATGT+TGG - Chr5:32081894-32081913 None:intergenic 30.0%
!! AAGAGAGATGCTTTTTATCA+AGG + Chr5:32081766-32081785 MsG0580026193.01.T01:intron 30.0%
ACCACGAGAAATATGCTTAT+AGG + Chr5:32082366-32082385 MsG0580026193.01.T01:CDS 35.0%
ATCAAGAAAAGAACTCATCG+AGG + Chr5:32082053-32082072 MsG0580026193.01.T01:CDS 35.0%
CTTCACAAAAAAGGGAATGT+TGG + Chr5:32082499-32082518 MsG0580026193.01.T01:CDS 35.0%
GCAAAAATTGCCAAAGATAC+TGG + Chr5:32082331-32082350 MsG0580026193.01.T01:CDS 35.0%
GTTGAAGAATTCACTTCAAG+TGG - Chr5:32081351-32081370 None:intergenic 35.0%
TACTGTCTTGACATTTGGAT+CGG + Chr5:32082455-32082474 MsG0580026193.01.T01:CDS 35.0%
TCAATAAGGCTTGAAGATGA+GGG - Chr5:32082117-32082136 None:intergenic 35.0%
! ATGAGTACTGTCTTGACATT+TGG + Chr5:32082450-32082469 MsG0580026193.01.T01:CDS 35.0%
!! ACTCATTTGATGATGATGTG+AGG - Chr5:32082436-32082455 None:intergenic 35.0%
!!! GAGTTTTCAGGTTCTCAATA+AGG - Chr5:32082131-32082150 None:intergenic 35.0%
AAAAGAACTCATCGAGGATG+AGG + Chr5:32082059-32082078 MsG0580026193.01.T01:CDS 40.0%
AGAAATATGCTTATAGGCGC+AGG + Chr5:32082372-32082391 MsG0580026193.01.T01:CDS 40.0%
ATCTTGGACATGGAACAACT+TGG - Chr5:32082245-32082264 None:intergenic 40.0%
ATGTCCAAGATGCAAAAGCA+CGG + Chr5:32082254-32082273 MsG0580026193.01.T01:CDS 40.0%
ATTCTGAATCCTCTGTGGTT+TGG - Chr5:32082092-32082111 None:intergenic 40.0%
CAAATAGAATCACCATGGCA+TGG - Chr5:32081406-32081425 None:intergenic 40.0%
CGTTCTCCCAAAAAGCTTTA+TGG - Chr5:32081208-32081227 None:intergenic 40.0%
CTCAATAAGGCTTGAAGATG+AGG - Chr5:32082118-32082137 None:intergenic 40.0%
CTCGATGTTTGTGTATGTGT+CGG + Chr5:32081934-32081953 MsG0580026193.01.T01:intron 40.0%
GCCTATAAGCATATTTCTCG+TGG - Chr5:32082370-32082389 None:intergenic 40.0%
TGAAAGAGATCTGTCCATCA+AGG + Chr5:32081708-32081727 MsG0580026193.01.T01:intron 40.0%
TGAGTTTCACTGCGATTTCT+CGG + Chr5:32081537-32081556 MsG0580026193.01.T01:intron 40.0%
! ACAACTTGGTCTGGTTTCTT+TGG - Chr5:32082231-32082250 None:intergenic 40.0%
! TTTCAGATGAGGGAGTTTTC+AGG - Chr5:32082143-32082162 None:intergenic 40.0%
!!! CAATTTTTGCAGAAGTGTCG+AGG - Chr5:32082321-32082340 None:intergenic 40.0%
AGACGTCTCTGTTTCAGATG+AGG - Chr5:32082154-32082173 None:intergenic 45.0%
AGTTTCACTGCGATTTCTCG+GGG + Chr5:32081539-32081558 MsG0580026193.01.T01:intron 45.0%
ATAAGGTATGTGCCATGCCA+TGG + Chr5:32081391-32081410 MsG0580026193.01.T01:intron 45.0%
CAAAGATACTGGACTTCTGG+AGG + Chr5:32082342-32082361 MsG0580026193.01.T01:CDS 45.0%
CTCCAGAAGTCCAGTATCTT+TGG - Chr5:32082344-32082363 None:intergenic 45.0%
GACGTCTCTGTTTCAGATGA+GGG - Chr5:32082153-32082172 None:intergenic 45.0%
GAGTTTCACTGCGATTTCTC+GGG + Chr5:32081538-32081557 MsG0580026193.01.T01:intron 45.0%
TGCCAAAGATACTGGACTTC+TGG + Chr5:32082339-32082358 MsG0580026193.01.T01:CDS 45.0%
! GTGCTTTTGCATCTTGGACA+TGG - Chr5:32082255-32082274 None:intergenic 45.0%
GGACATGGAACAACTTGGTC+TGG - Chr5:32082240-32082259 None:intergenic 50.0%
GGCTTCATTCCAAACCACAG+AGG + Chr5:32082080-32082099 MsG0580026193.01.T01:CDS 50.0%
GGGAGATTCTGAATCCTCTG+TGG - Chr5:32082097-32082116 None:intergenic 50.0%
! GTGTCCGTGCTTTTGCATCT+TGG - Chr5:32082261-32082280 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 32081189 32082530 32081189 ID=MsG0580026193.01;Name=MsG0580026193.01
Chr5 mRNA 32081189 32082530 32081189 ID=MsG0580026193.01.T01;Parent=MsG0580026193.01;Name=MsG0580026193.01.T01;_AED=0.41;_eAED=0.42;_QI=0|0|0|1|0|0|2|0|229
Chr5 exon 32081189 32081395 32081189 ID=MsG0580026193.01.T01:exon:13314;Parent=MsG0580026193.01.T01
Chr5 exon 32082048 32082530 32082048 ID=MsG0580026193.01.T01:exon:13315;Parent=MsG0580026193.01.T01
Chr5 CDS 32081189 32081395 32081189 ID=MsG0580026193.01.T01:cds;Parent=MsG0580026193.01.T01
Chr5 CDS 32082048 32082530 32082048 ID=MsG0580026193.01.T01:cds;Parent=MsG0580026193.01.T01
Chr5 mRNA 32082210 32082530 32082210 ID=MsG0580026193.01.T02;Parent=MsG0580026193.01;Name=MsG0580026193.01.T02;_AED=0.46;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|106
Chr5 exon 32082210 32082530 32082210 ID=MsG0580026193.01.T02:exon:13316;Parent=MsG0580026193.01.T02
Chr5 CDS 32082210 32082530 32082210 ID=MsG0580026193.01.T02:cds;Parent=MsG0580026193.01.T02
Gene Sequence

>MsG0580026193.01.T01

ATGATAAACAATTCAACCATAAAGCTTTTTGGGAGAACGATTTTTCAAACACACAACACTGATGTGTCTATCAATGATTCTTCCTCAGAATTTACTCCTACTATTGCTCATGAAGATTTCTCTGATCATAGTAGTCATTCATCTTTATCTGCATCTTCTCCACTTGAAGTGAATTCTTCAACTGAACATGATGCAAAGAGATATAAGGAAACATCAAGAAAAGAACTCATCGAGGATGAGGCTTCATTCCAAACCACAGAGGATTCAGAATCTCCCTCATCTTCAAGCCTTATTGAGAACCTGAAAACTCCCTCATCTGAAACAGAGACGTCTCAATCAATTTCCACTAAAATTGATGAACAAACTGATATGTCACAAGAAAAATCTCCAAAGAAACCAGACCAAGTTGTTCCATGTCCAAGATGCAAAAGCACGGACACAAAATTTTGCTACTACAACAACTACAACGTCAAACAGCCTCGACACTTCTGCAAAAATTGCCAAAGATACTGGACTTCTGGAGGAACCACGAGAAATATGCTTATAGGCGCAGGTCGCCGCAAGAACAGAATCTCTTTTTTTCATTCTGATACCTCACATCATCATCAAATGAGTACTGTCTTGACATTTGGATCGGATTCTCCTATTATGTCTTCAACTTCACAAAAAAGGGAATGTTGGTTCACTTGA

>MsG0580026193.01.T02

ATGTCACAAGAAAAATCTCCAAAGAAACCAGACCAAGTTGTTCCATGTCCAAGATGCAAAAGCACGGACACAAAATTTTGCTACTACAACAACTACAACGTCAAACAGCCTCGACACTTCTGCAAAAATTGCCAAAGATACTGGACTTCTGGAGGAACCACGAGAAATATGCTTATAGGCGCAGGTCGCCGCAAGAACAGAATCTCTTTTTTTCATTCTGATACCTCACATCATCATCAAATGAGTACTGTCTTGACATTTGGATCGGATTCTCCTATTATGTCTTCAACTTCACAAAAAAGGGAATGTTGGTTCACTTGA
Protein sequence

>MsG0580026193.01.T01

MINNSTIKLFGRTIFQTHNTDVSINDSSSEFTPTIAHEDFSDHSSHSSLSASSPLEVNSSTEHDAKRYKETSRKELIEDEASFQTTEDSESPSSSSLIENLKTPSSETETSQSISTKIDEQTDMSQEKSPKKPDQVVPCPRCKSTDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGTTRNMLIGAGRRKNRISFFHSDTSHHHQMSTVLTFGSDSPIMSSTSQKRECWFT*

>MsG0580026193.01.T02

MSQEKSPKKPDQVVPCPRCKSTDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGTTRNMLIGAGRRKNRISFFHSDTSHHHQMSTVLTFGSDSPIMSSTSQKRECWFT*