Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026293.01.T01 | GAU43339.1 | 59.036 | 83 | 34 | 0 | 1 | 83 | 67 | 149 | 4.70E-24 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026293.01.T01 | A0A2Z6PEJ1 | 59.036 | 83 | 34 | 0 | 1 | 83 | 67 | 149 | 2.25e-24 | 104 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580026293.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026293.01.T01 | MTR_0060s0090 | 53.521 | 71 | 33 | 0 | 12 | 82 | 59 | 129 | 3.42e-22 | 85.1 |
| MsG0580026293.01.T01 | MTR_1g070355 | 51.389 | 72 | 35 | 0 | 12 | 83 | 77 | 148 | 1.84e-21 | 87.8 |
| MsG0580026293.01.T01 | MTR_3g448650 | 43.373 | 83 | 47 | 0 | 1 | 83 | 74 | 156 | 1.01e-20 | 81.6 |
| MsG0580026293.01.T01 | MTR_2g101460 | 42.169 | 83 | 48 | 0 | 1 | 83 | 37 | 119 | 9.20e-17 | 72.0 |
| MsG0580026293.01.T01 | MTR_2g101460 | 42.169 | 83 | 48 | 0 | 1 | 83 | 47 | 129 | 1.59e-16 | 71.6 |
| MsG0580026293.01.T01 | MTR_1g052260 | 40.964 | 83 | 49 | 0 | 5 | 87 | 72 | 154 | 6.49e-16 | 68.9 |
| MsG0580026293.01.T01 | MTR_5g076070 | 48.571 | 70 | 36 | 0 | 14 | 83 | 58 | 127 | 1.46e-15 | 70.1 |
| MsG0580026293.01.T01 | MTR_6g463250 | 42.169 | 83 | 33 | 2 | 1 | 83 | 80 | 147 | 5.18e-15 | 67.0 |
| MsG0580026293.01.T01 | MTR_6g053140 | 46.667 | 75 | 33 | 2 | 8 | 82 | 102 | 169 | 2.06e-13 | 63.5 |
| MsG0580026293.01.T01 | MTR_6g022080 | 36.842 | 76 | 48 | 0 | 8 | 83 | 84 | 159 | 8.06e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTTCTAATGAGGGTTAC+AGG | 0.288446 | 5:+34238938 | MsG0580026293.01.T01:CDS |
| TGACGTCTTGGTTCACGCTT+TGG | 0.373066 | 5:-34238988 | None:intergenic |
| AATATTAGCATTGAAGATGT+TGG | 0.397161 | 5:+34238784 | MsG0580026293.01.T01:CDS |
| AACATTTGTTTGTTCTAATG+AGG | 0.413090 | 5:+34238930 | MsG0580026293.01.T01:CDS |
| TCCGCAAAAGCAAGCTTGTT+AGG | 0.420307 | 5:+34238881 | MsG0580026293.01.T01:CDS |
| CTGCATATCAATTCTATAGT+TGG | 0.443998 | 5:+34238833 | MsG0580026293.01.T01:CDS |
| CTTGGTTCACGCTTTGGAGT+TGG | 0.445770 | 5:-34238982 | None:intergenic |
| AGTTGGTATTCAAGATTGAA+TGG | 0.446191 | 5:+34238850 | MsG0580026293.01.T01:CDS |
| CCACACCTACTTTGACGTCT+TGG | 0.457690 | 5:-34239000 | None:intergenic |
| ATATTAGCATTGAAGATGTT+GGG | 0.464592 | 5:+34238785 | MsG0580026293.01.T01:CDS |
| CAAGCTTGTTAGGAATGTAA+AGG | 0.494002 | 5:+34238891 | MsG0580026293.01.T01:CDS |
| AAGCTTGTTAGGAATGTAAA+GGG | 0.507228 | 5:+34238892 | MsG0580026293.01.T01:CDS |
| ACATTTGTTTGTTCTAATGA+GGG | 0.540461 | 5:+34238931 | MsG0580026293.01.T01:CDS |
| TTGTTCTAATGAGGGTTACA+GGG | 0.548367 | 5:+34238939 | MsG0580026293.01.T01:CDS |
| TTAATATTCATACAGAAAGA+TGG | 0.561841 | 5:+34239048 | MsG0580026293.01.T01:CDS |
| GTGAACCAAGACGTCAAAGT+AGG | 0.607924 | 5:+34238995 | MsG0580026293.01.T01:CDS |
| CCAAGACGTCAAAGTAGGTG+TGG | 0.628380 | 5:+34239000 | MsG0580026293.01.T01:CDS |
| TTAGCATTGAAGATGTTGGG+AGG | 0.679576 | 5:+34238788 | MsG0580026293.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATATTCATACAGAAAGA+TGG | + | Chr5:34239048-34239067 | MsG0580026293.01.T01:CDS | 20.0% |
| ! | AACATTTGTTTGTTCTAATG+AGG | + | Chr5:34238930-34238949 | MsG0580026293.01.T01:CDS | 25.0% |
| ! | ACATTTGTTTGTTCTAATGA+GGG | + | Chr5:34238931-34238950 | MsG0580026293.01.T01:CDS | 25.0% |
| !! | AATATTAGCATTGAAGATGT+TGG | + | Chr5:34238784-34238803 | MsG0580026293.01.T01:CDS | 25.0% |
| !! | ATATTAGCATTGAAGATGTT+GGG | + | Chr5:34238785-34238804 | MsG0580026293.01.T01:CDS | 25.0% |
| CTGCATATCAATTCTATAGT+TGG | + | Chr5:34238833-34238852 | MsG0580026293.01.T01:CDS | 30.0% | |
| ! | AGTTGGTATTCAAGATTGAA+TGG | + | Chr5:34238850-34238869 | MsG0580026293.01.T01:CDS | 30.0% |
| !! | AAGCTTGTTAGGAATGTAAA+GGG | + | Chr5:34238892-34238911 | MsG0580026293.01.T01:CDS | 30.0% |
| TTGTTCTAATGAGGGTTACA+GGG | + | Chr5:34238939-34238958 | MsG0580026293.01.T01:CDS | 35.0% | |
| TTTGTTCTAATGAGGGTTAC+AGG | + | Chr5:34238938-34238957 | MsG0580026293.01.T01:CDS | 35.0% | |
| !! | CAAGCTTGTTAGGAATGTAA+AGG | + | Chr5:34238891-34238910 | MsG0580026293.01.T01:CDS | 35.0% |
| ! | GTCAAAGTAGGTGTGGTTTT+TGG | + | Chr5:34239007-34239026 | MsG0580026293.01.T01:CDS | 40.0% |
| ! | TTAGCATTGAAGATGTTGGG+AGG | + | Chr5:34238788-34238807 | MsG0580026293.01.T01:CDS | 40.0% |
| !! | TCCTAACAAGCTTGCTTTTG+CGG | - | Chr5:34238885-34238904 | None:intergenic | 40.0% |
| GTGAACCAAGACGTCAAAGT+AGG | + | Chr5:34238995-34239014 | MsG0580026293.01.T01:CDS | 45.0% | |
| ! | TCCGCAAAAGCAAGCTTGTT+AGG | + | Chr5:34238881-34238900 | MsG0580026293.01.T01:CDS | 45.0% |
| CCAAGACGTCAAAGTAGGTG+TGG | + | Chr5:34239000-34239019 | MsG0580026293.01.T01:CDS | 50.0% | |
| CCACACCTACTTTGACGTCT+TGG | - | Chr5:34239003-34239022 | None:intergenic | 50.0% | |
| TGACGTCTTGGTTCACGCTT+TGG | - | Chr5:34238991-34239010 | None:intergenic | 50.0% | |
| !! | CTTGGTTCACGCTTTGGAGT+TGG | - | Chr5:34238985-34239004 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 34238775 | 34239077 | 34238775 | ID=MsG0580026293.01;Name=MsG0580026293.01 |
| Chr5 | mRNA | 34238775 | 34239077 | 34238775 | ID=MsG0580026293.01.T01;Parent=MsG0580026293.01;Name=MsG0580026293.01.T01;_AED=0.43;_eAED=0.53;_QI=0|-1|0|1|-1|1|1|0|100 |
| Chr5 | exon | 34238775 | 34239077 | 34238775 | ID=MsG0580026293.01.T01:exon:13856;Parent=MsG0580026293.01.T01 |
| Chr5 | CDS | 34238775 | 34239077 | 34238775 | ID=MsG0580026293.01.T01:cds;Parent=MsG0580026293.01.T01 |
| Gene Sequence |
| Protein sequence |
