Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026297.01.T01 | GAU47019.1 | 60.87 | 92 | 29 | 3 | 1 | 87 | 1 | 90 | 7.36E-23 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026297.01.T01 | A0A2Z6PKX5 | 60.870 | 92 | 29 | 3 | 1 | 87 | 1 | 90 | 3.51e-23 | 100 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580026297.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026297.01.T01 | MTR_2g049530 | 55.789 | 95 | 35 | 1 | 1 | 88 | 1 | 95 | 9.80e-27 | 97.1 |
| MsG0580026297.01.T01 | MTR_2g049630 | 60.000 | 95 | 29 | 3 | 1 | 87 | 1 | 94 | 1.02e-25 | 97.8 |
| MsG0580026297.01.T01 | MTR_2g049610 | 54.839 | 93 | 34 | 3 | 1 | 88 | 1 | 90 | 6.06e-24 | 90.9 |
| MsG0580026297.01.T01 | MTR_4g045977 | 46.237 | 93 | 43 | 3 | 1 | 87 | 1 | 92 | 5.61e-19 | 79.7 |
| MsG0580026297.01.T01 | MTR_4g107170 | 46.237 | 93 | 43 | 3 | 1 | 87 | 1 | 92 | 1.06e-18 | 77.4 |
| MsG0580026297.01.T01 | MTR_1g115290 | 51.724 | 58 | 24 | 2 | 33 | 87 | 2 | 58 | 1.80e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCACATCAAGTTCCTT+TGG | 0.332239 | 5:-34319678 | None:intergenic |
| GCTTTCGTTCAGATAAAGAA+TGG | 0.439264 | 5:+34319489 | MsG0580026297.01.T01:CDS |
| ATACAAGCAACAAAAGATTG+AGG | 0.523829 | 5:+34319647 | MsG0580026297.01.T01:CDS |
| TCCAAAGGAACTTGATGTGA+AGG | 0.531441 | 5:+34319677 | MsG0580026297.01.T01:CDS |
| TACAAGCAACAAAAGATTGA+GGG | 0.532621 | 5:+34319648 | MsG0580026297.01.T01:CDS |
| GAGCATGGACCGTACAATTG+TGG | 0.544256 | 5:-34319619 | None:intergenic |
| AACAAGACTCCACAATTGTA+CGG | 0.574036 | 5:+34319610 | MsG0580026297.01.T01:CDS |
| GATTGAGGGTGCAACTCCAA+AGG | 0.606789 | 5:+34319662 | MsG0580026297.01.T01:CDS |
| ATGATAGTAGATCAATGACT+TGG | 0.624411 | 5:+34319586 | MsG0580026297.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTAAATTTTCTAGCAAGTT+TGG | + | Chr5:34319528-34319547 | MsG0580026297.01.T01:CDS | 25.0% |
| ATACAAGCAACAAAAGATTG+AGG | + | Chr5:34319647-34319666 | MsG0580026297.01.T01:CDS | 30.0% | |
| ATGATAGTAGATCAATGACT+TGG | + | Chr5:34319586-34319605 | MsG0580026297.01.T01:CDS | 30.0% | |
| TACAAGCAACAAAAGATTGA+GGG | + | Chr5:34319648-34319667 | MsG0580026297.01.T01:CDS | 30.0% | |
| AACAAGACTCCACAATTGTA+CGG | + | Chr5:34319610-34319629 | MsG0580026297.01.T01:CDS | 35.0% | |
| GCTTTCGTTCAGATAAAGAA+TGG | + | Chr5:34319489-34319508 | MsG0580026297.01.T01:CDS | 35.0% | |
| !!! | TTGCTTGTATTTTTCGAGCA+TGG | - | Chr5:34319637-34319656 | None:intergenic | 35.0% |
| TCCAAAGGAACTTGATGTGA+AGG | + | Chr5:34319677-34319696 | MsG0580026297.01.T01:CDS | 40.0% | |
| TCCTTCACATCAAGTTCCTT+TGG | - | Chr5:34319681-34319700 | None:intergenic | 40.0% | |
| GAGCATGGACCGTACAATTG+TGG | - | Chr5:34319622-34319641 | None:intergenic | 50.0% | |
| ! | GATTGAGGGTGCAACTCCAA+AGG | + | Chr5:34319662-34319681 | MsG0580026297.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 34319447 | 34319716 | 34319447 | ID=MsG0580026297.01;Name=MsG0580026297.01 |
| Chr5 | mRNA | 34319447 | 34319716 | 34319447 | ID=MsG0580026297.01.T01;Parent=MsG0580026297.01;Name=MsG0580026297.01.T01;_AED=0.29;_eAED=0.31;_QI=0|-1|0|1|-1|0|1|0|89 |
| Chr5 | exon | 34319447 | 34319716 | 34319447 | ID=MsG0580026297.01.T01:exon:25470;Parent=MsG0580026297.01.T01 |
| Chr5 | CDS | 34319447 | 34319716 | 34319447 | ID=MsG0580026297.01.T01:cds;Parent=MsG0580026297.01.T01 |
| Gene Sequence |
| Protein sequence |
