AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026846.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026846.01.T01 MTR_3g091700 66.082 171 51 4 160 324 92 261 9.14e-67 210
MsG0580026846.01.T01 MTR_3g435550 50.467 107 47 1 222 322 64 170 4.32e-28 108
MsG0580026846.01.T01 MTR_5g024070 33.835 133 68 2 205 317 182 314 7.40e-17 80.5
MsG0580026846.01.T01 MTR_4g053370 41.611 149 19 6 8 93 17 160 9.64e-17 80.5
MsG0580026846.01.T01 MTR_3g091720 65.385 52 11 1 273 324 19 63 3.65e-15 69.3
MsG0580026846.01.T01 MTR_0140s0010 42.857 70 31 1 247 307 150 219 8.25e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026846.01.T01 AT5G53040 52.381 105 50 0 214 318 145 249 5.38e-31 117
MsG0580026846.01.T01 AT5G66990 50.450 111 44 1 222 321 164 274 5.14e-30 115
MsG0580026846.01.T01 AT1G18790 48.246 114 49 1 215 318 126 239 1.49e-26 106
MsG0580026846.01.T01 AT1G74480 48.544 103 43 1 222 314 140 242 2.35e-25 103
MsG0580026846.01.T01 AT4G35590 35.507 138 66 3 204 318 169 306 1.34e-14 73.9
MsG0580026846.01.T01 AT4G35590 35.507 138 66 3 204 318 219 356 2.80e-14 73.2

Find 104 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTAGCAGCCACGTTTAT+AGG 0.243887 5:-46324440 None:intergenic
TCAGTGGAATGCCCCATTTC+AGG 0.244696 5:-46324047 None:intergenic
GGTGGTGGTGCGAGGGTTGT+TGG 0.285241 5:-46323810 None:intergenic
GGTTGCAATTTGATGATATA+AGG 0.299267 5:+46324402 MsG0580026846.01.T01:CDS
AACTGCTTTCATCGAGGTTT+GGG 0.299819 5:-46323998 None:intergenic
TCTAGGAATAGTCTCTGATT+TGG 0.307887 5:+46325078 MsG0580026846.01.T01:three_prime_UTR
CTAGGAATAGTCTCTGATTT+GGG 0.327957 5:+46325079 MsG0580026846.01.T01:three_prime_UTR
ACGTGTTGGGCTTGTGGGTC+CGG 0.336693 5:-46323909 None:intergenic
AAGGAAGGAGAGGGTGGTGG+TGG 0.338861 5:-46323828 None:intergenic
ACGGTGGTGGGTGGTGAGTT+TGG 0.340603 5:-46323783 None:intergenic
GGTTGTTGGGCCGACGGTGG+TGG 0.346146 5:-46323796 None:intergenic
CGGGAACGTGTTGGGCTTGT+GGG 0.352995 5:-46323914 None:intergenic
ATTGGACGGTAGGTTGGTGG+TGG 0.364075 5:-46323867 None:intergenic
AAACTGCTTTCATCGAGGTT+TGG 0.367149 5:-46323999 None:intergenic
GAAGGAGAGGGTGGTGGTGG+TGG 0.375077 5:-46323825 None:intergenic
GCTTCCGATAAAACAGGAAA+TGG 0.376610 5:+46324104 MsG0580026846.01.T01:CDS
TTGTGGGTCCGGTGGGATCG+CGG 0.386823 5:-46323898 None:intergenic
TGGTGGTGGGGAGATTTCTT+CGG 0.406147 5:-46323853 None:intergenic
GTGGTGGTGCGAGGGTTGTT+GGG 0.411312 5:-46323809 None:intergenic
TTTGCGGTCGGGAACGTGTT+GGG 0.411944 5:-46323922 None:intergenic
GTTGAGGCTTCCGATAAAAC+AGG 0.414081 5:+46324098 MsG0580026846.01.T01:CDS
GTTAGAGAAATTTCCTAAAA+CGG 0.416699 5:+46324712 MsG0580026846.01.T01:CDS
TCGGGAACGTGTTGGGCTTG+TGG 0.424341 5:-46323915 None:intergenic
TTACCTGTACTCTTTGTATG+AGG 0.429507 5:-46324876 None:intergenic
GCAAAAGAAGTTGAAGTTCA+AGG 0.431814 5:-46324815 None:intergenic
TGTTGTGTGATGCAGGAAAT+GGG 0.433682 5:+46324647 MsG0580026846.01.T01:intron
TTTCATCGGTGGCAAGAATC+TGG 0.434558 5:+46325041 MsG0580026846.01.T01:three_prime_UTR
TGTGGGTCCGGTGGGATCGC+GGG 0.445023 5:-46323897 None:intergenic
AAGTTGAAGTTCAAGGGTTG+AGG 0.447164 5:-46324808 None:intergenic
TGGACGGTAGGTTGGTGGTG+GGG 0.450989 5:-46323865 None:intergenic
CCTCGATGAAAGCAGTTTAG+AGG 0.454156 5:+46324004 MsG0580026846.01.T01:CDS
CTGCTACTTGCCAATGGTAA+TGG 0.457624 5:-46324350 None:intergenic
GGGATCGCGGGAGCGAGGAT+TGG 0.462338 5:-46323885 None:intergenic
GAGAAGGAAGGAGAGGGTGG+TGG 0.466197 5:-46323831 None:intergenic
TCCTTCTCCGATTGGTGGCA+AGG 0.466589 5:-46323954 None:intergenic
ATATCTTTGTATCGATTGAC+TGG 0.469171 5:+46325112 MsG0580026846.01.T01:three_prime_UTR
TGTTGGGCTTGTGGGTCCGG+TGG 0.470152 5:-46323906 None:intergenic
GTTGTTGGGCCGACGGTGGT+GGG 0.472127 5:-46323795 None:intergenic
CGTGAGAATGCTGAGGAAGA+AGG 0.473231 5:-46323708 None:intergenic
CTCCGATCCTTGCCACCAAT+CGG 0.479360 5:+46323947 MsG0580026846.01.T01:CDS
AGTTGAAGTTCAAGGGTTGA+GGG 0.480361 5:-46324807 None:intergenic
TCAATTTGATCCCAATCATA+AGG 0.481581 5:-46324956 None:intergenic
TTGGACGGTAGGTTGGTGGT+GGG 0.486120 5:-46323866 None:intergenic
TTTGTTCCTTCTCCGATTGG+TGG 0.486927 5:-46323959 None:intergenic
CTCCGATTGGTGGCAAGGAT+CGG 0.487689 5:-46323949 None:intergenic
TTGGGATCAAATTGAGTAGC+TGG 0.493112 5:+46324964 MsG0580026846.01.T01:three_prime_UTR
TGCGAGGGTTGTTGGGCCGA+CGG 0.495297 5:-46323802 None:intergenic
TCGATACAAAGATATGTTCA+AGG 0.495811 5:-46325104 None:intergenic
GACAAACGAGGTTGCTATGT+TGG 0.496956 5:+46324673 MsG0580026846.01.T01:CDS
ATGAGCAATGTAAATACTGT+TGG 0.499277 5:+46325182 MsG0580026846.01.T01:three_prime_UTR
TCAAGAATTGCCATTACCAT+TGG 0.504208 5:+46324340 MsG0580026846.01.T01:CDS
GCGAGGATTGGACGGTAGGT+TGG 0.504638 5:-46323873 None:intergenic
GCTTCGGCGAACACTGAAAG+TGG 0.505718 5:+46324281 MsG0580026846.01.T01:CDS
CTGAAGCACACTGCTTTCAT+CGG 0.507113 5:+46325027 MsG0580026846.01.T01:three_prime_UTR
GTTGGGCTTGTGGGTCCGGT+GGG 0.510955 5:-46323905 None:intergenic
GCTTCGGCGAACACTGAAAG+TGG 0.511245 5:+46324218 MsG0580026846.01.T01:CDS
CAAAAGAAGTTGAAGTTCAA+GGG 0.513754 5:-46324814 None:intergenic
GTGCTAGTACTGGTATCAAA+AGG 0.513912 5:+46324375 MsG0580026846.01.T01:CDS
CTTCCGATAAAACAGGAAAT+GGG 0.515746 5:+46324105 MsG0580026846.01.T01:CDS
GTTGGGCCGACGGTGGTGGG+TGG 0.526975 5:-46323792 None:intergenic
GAGTACAGGTAAGGACTCAT+TGG 0.528283 5:+46324887 MsG0580026846.01.T01:three_prime_UTR
AGGATTGGACGGTAGGTTGG+TGG 0.535192 5:-46323870 None:intergenic
AGTAGCAGCAGTGCTAGTAC+TGG 0.539670 5:+46324365 MsG0580026846.01.T01:CDS
GGGAGCGAGGATTGGACGGT+AGG 0.548033 5:-46323877 None:intergenic
TGGGGAGATTTCTTCGGAGA+AGG 0.548037 5:-46323847 None:intergenic
CCTCGCTCCCGCGATCCCAC+CGG 0.548146 5:+46323890 MsG0580026846.01.T01:CDS
CCGGTGGGATCGCGGGAGCG+AGG 0.551828 5:-46323890 None:intergenic
TTCTTCGGAGAAGGAAGGAG+AGG 0.552426 5:-46323838 None:intergenic
AAGCCTCATACAAAGAGTAC+AGG 0.556908 5:+46324873 MsG0580026846.01.T01:three_prime_UTR
TGTGATTCTTCTGACATGGT+TGG 0.562128 5:-46323750 None:intergenic
GAGATTTCTTCGGAGAAGGA+AGG 0.563850 5:-46323843 None:intergenic
ACATTGAGTGCTGCGGAGAG+TGG 0.564968 5:+46324248 MsG0580026846.01.T01:CDS
GCTGCTAAGAAAATGAAAGT+TGG 0.567685 5:+46324452 MsG0580026846.01.T01:CDS
GTGAGAATGCTGAGGAAGAA+GGG 0.575482 5:-46323707 None:intergenic
TCCTTGCCACCAATCGGAGA+AGG 0.578994 5:+46323953 MsG0580026846.01.T01:CDS
ACATCACTAGGTGGCCTCAC+AGG 0.583382 5:+46324510 MsG0580026846.01.T01:CDS
TCGCGGGAGCGAGGATTGGA+CGG 0.584567 5:-46323881 None:intergenic
AACTCACCACCCACCACCGT+CGG 0.589477 5:+46323786 MsG0580026846.01.T01:CDS
GTACTGGTATCAAAAGGAAG+AGG 0.590058 5:+46324381 MsG0580026846.01.T01:CDS
TCATACAAAGAGTACAGGTA+AGG 0.591336 5:+46324878 MsG0580026846.01.T01:three_prime_UTR
TGGGTGGTGAGTTTGGAGTG+CGG 0.593509 5:-46323776 None:intergenic
AAAGATAGTATTGCTGAGAG+TGG 0.600909 5:+46324071 MsG0580026846.01.T01:CDS
ACATTGAGTGCTGCGGAGAG+TGG 0.601225 5:+46324185 MsG0580026846.01.T01:CDS
CTTCGGCGAACACTGAAAGT+GGG 0.607053 5:+46324282 MsG0580026846.01.T01:CDS
GTGGTGTGATTCTTCTGACA+TGG 0.609508 5:-46323754 None:intergenic
GAGGGTTGTTGGGCCGACGG+TGG 0.612177 5:-46323799 None:intergenic
CTTCGGCGAACACTGAAAGT+GGG 0.613874 5:+46324219 MsG0580026846.01.T01:CDS
GCAGGGAGCTCAACATCACT+AGG 0.620032 5:+46324498 MsG0580026846.01.T01:CDS
TCTTCGGAGAAGGAAGGAGA+GGG 0.626899 5:-46323837 None:intergenic
AAGCACACTGCTTTCATCGG+TGG 0.627336 5:+46325030 MsG0580026846.01.T01:three_prime_UTR
TCGGAGAAGGAAGGAGAGGG+TGG 0.628913 5:-46323834 None:intergenic
GCACTGCTGCTACTTGCCAA+TGG 0.635767 5:-46324356 None:intergenic
AGCAATACTATCTTTGTCAG+TGG 0.636464 5:-46324063 None:intergenic
TGTACTCATCAACAACCGTA+AGG 0.637101 5:+46324550 MsG0580026846.01.T01:CDS
GTGGTGAGTTTGGAGTGCGG+TGG 0.646330 5:-46323773 None:intergenic
AGTGAAGCGTGAGAATGCTG+AGG 0.649378 5:-46323715 None:intergenic
GCACTCAATGTCAATGACAA+TGG 0.649619 5:-46324173 None:intergenic
CCTCTAAACTGCTTTCATCG+AGG 0.654147 5:-46324004 None:intergenic
GTCATTGACATTGAGTGCTG+CGG 0.658171 5:+46324178 MsG0580026846.01.T01:CDS
AGAACTATATTCTATTGACT+AGG 0.661218 5:+46324992 MsG0580026846.01.T01:three_prime_UTR
GTTTGTGACATTGAGTGCTG+CGG 0.662193 5:+46324241 MsG0580026846.01.T01:CDS
GGAAATGGGTTTGACAAACG+AGG 0.669583 5:+46324661 MsG0580026846.01.T01:intron
AAGCTGCTCAATTTCCTGTG+AGG 0.686396 5:-46324524 None:intergenic
GGGAGCTCAACATCACTAGG+TGG 0.707852 5:+46324501 MsG0580026846.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATTATTTTTTATCAAC+TGG + Chr5:46324604-46324623 MsG0580026846.01.T01:intron 10.0%
!!! ATTTTAGTCTTCTATTTTTT+TGG + Chr5:46324924-46324943 MsG0580026846.01.T01:three_prime_UTR 15.0%
!!! TTCTATTTTTTTGGTTTTTT+TGG + Chr5:46324933-46324952 MsG0580026846.01.T01:three_prime_UTR 15.0%
!!! TTTTGAATATGTGAAAACAA+TGG + Chr5:46325156-46325175 MsG0580026846.01.T01:three_prime_UTR 20.0%
! ATGAAAAAATCAAGAAGCTT+AGG + Chr5:46324741-46324760 MsG0580026846.01.T01:CDS 25.0%
! CAAAATCAACATATACCTTA+CGG - Chr5:46324568-46324587 None:intergenic 25.0%
! GTTAGAGAAATTTCCTAAAA+CGG + Chr5:46324712-46324731 MsG0580026846.01.T01:CDS 25.0%
!! AGAACTATATTCTATTGACT+AGG + Chr5:46324992-46325011 MsG0580026846.01.T01:three_prime_UTR 25.0%
!! TTTTCATTCAATTCCGTTTT+AGG - Chr5:46324728-46324747 None:intergenic 25.0%
!!! AAGGTATATGTTGATTTTGT+TGG + Chr5:46324569-46324588 MsG0580026846.01.T01:intron 25.0%
!!! ATTTTTTATCAACTGGTCAT+AGG + Chr5:46324611-46324630 MsG0580026846.01.T01:intron 25.0%
!!! TCTTTTGAAAAATAGATGCA+GGG + Chr5:46324481-46324500 MsG0580026846.01.T01:CDS 25.0%
ATGAGCAATGTAAATACTGT+TGG + Chr5:46325182-46325201 MsG0580026846.01.T01:three_prime_UTR 30.0%
CAAAAGAAGTTGAAGTTCAA+GGG - Chr5:46324817-46324836 None:intergenic 30.0%
TCAATTTGATCCCAATCATA+AGG - Chr5:46324959-46324978 None:intergenic 30.0%
TCGATACAAAGATATGTTCA+AGG - Chr5:46325107-46325126 None:intergenic 30.0%
! AAACCCATTTCCTGTTTTAT+CGG - Chr5:46324111-46324130 None:intergenic 30.0%
! GGTTGCAATTTGATGATATA+AGG + Chr5:46324402-46324421 MsG0580026846.01.T01:CDS 30.0%
! TTTTGATGTTCCTATAAACG+TGG + Chr5:46324430-46324449 MsG0580026846.01.T01:CDS 30.0%
!! ATATCTTTGTATCGATTGAC+TGG + Chr5:46325112-46325131 MsG0580026846.01.T01:three_prime_UTR 30.0%
!! ATTATTTTGTTGTGTGATGC+AGG + Chr5:46324640-46324659 MsG0580026846.01.T01:intron 30.0%
!! CTCTTTTGAAAAATAGATGC+AGG + Chr5:46324480-46324499 MsG0580026846.01.T01:CDS 30.0%
!!! AAAGCTTGTTTTACCTGAAA+TGG + Chr5:46324034-46324053 MsG0580026846.01.T01:CDS 30.0%
!!! AAGCTTGTTTTACCTGAAAT+GGG + Chr5:46324035-46324054 MsG0580026846.01.T01:CDS 30.0%
!!! GTTTTTTTGGCCTTATGATT+GGG + Chr5:46324946-46324965 MsG0580026846.01.T01:three_prime_UTR 30.0%
!!! TGGTCTATGATTTTTTCTCT+AGG + Chr5:46325061-46325080 MsG0580026846.01.T01:three_prime_UTR 30.0%
AAAGATAGTATTGCTGAGAG+TGG + Chr5:46324071-46324090 MsG0580026846.01.T01:CDS 35.0%
AGCAATACTATCTTTGTCAG+TGG - Chr5:46324066-46324085 None:intergenic 35.0%
CGGAGAAGGAACAAAAAATA+GGG + Chr5:46323967-46323986 MsG0580026846.01.T01:CDS 35.0%
CTAGGAATAGTCTCTGATTT+GGG + Chr5:46325079-46325098 MsG0580026846.01.T01:three_prime_UTR 35.0%
CTTCCGATAAAACAGGAAAT+GGG + Chr5:46324105-46324124 MsG0580026846.01.T01:CDS 35.0%
GCAAAAGAAGTTGAAGTTCA+AGG - Chr5:46324818-46324837 None:intergenic 35.0%
GCTGCTAAGAAAATGAAAGT+TGG + Chr5:46324452-46324471 MsG0580026846.01.T01:CDS 35.0%
TCAAGAATTGCCATTACCAT+TGG + Chr5:46324340-46324359 MsG0580026846.01.T01:CDS 35.0%
TCGGAGAAGGAACAAAAAAT+AGG + Chr5:46323966-46323985 MsG0580026846.01.T01:CDS 35.0%
TCTAGGAATAGTCTCTGATT+TGG + Chr5:46325078-46325097 MsG0580026846.01.T01:three_prime_UTR 35.0%
TTACCTGTACTCTTTGTATG+AGG - Chr5:46324879-46324898 None:intergenic 35.0%
! ACTTCTTTTGCTTGTAGTGT+TGG + Chr5:46324827-46324846 MsG0580026846.01.T01:three_prime_UTR 35.0%
! TCATACAAAGAGTACAGGTA+AGG + Chr5:46324878-46324897 MsG0580026846.01.T01:three_prime_UTR 35.0%
!!! AGCTTGTTTTACCTGAAATG+GGG + Chr5:46324036-46324055 MsG0580026846.01.T01:CDS 35.0%
!!! GGTTTTTTTGGCCTTATGAT+TGG + Chr5:46324945-46324964 MsG0580026846.01.T01:three_prime_UTR 35.0%
!!! TTTTTTGTTCCTTCTCCGAT+TGG - Chr5:46323965-46323984 None:intergenic 35.0%
AAACTGCTTTCATCGAGGTT+TGG - Chr5:46324002-46324021 None:intergenic 40.0%
AACTGCTTTCATCGAGGTTT+GGG - Chr5:46324001-46324020 None:intergenic 40.0%
AAGTTGAAGTTCAAGGGTTG+AGG - Chr5:46324811-46324830 None:intergenic 40.0%
AGTTGAAGTTCAAGGGTTGA+GGG - Chr5:46324810-46324829 None:intergenic 40.0%
GCACTCAATGTCAATGACAA+TGG - Chr5:46324176-46324195 None:intergenic 40.0%
GCTTCCGATAAAACAGGAAA+TGG + Chr5:46324104-46324123 MsG0580026846.01.T01:CDS 40.0%
TGTACTCATCAACAACCGTA+AGG + Chr5:46324550-46324569 MsG0580026846.01.T01:CDS 40.0%
TGTGATTCTTCTGACATGGT+TGG - Chr5:46323753-46323772 None:intergenic 40.0%
TGTTGTGTGATGCAGGAAAT+GGG + Chr5:46324647-46324666 MsG0580026846.01.T01:intron 40.0%
TTCTTAGCAGCCACGTTTAT+AGG - Chr5:46324443-46324462 None:intergenic 40.0%
TTGGGATCAAATTGAGTAGC+TGG + Chr5:46324964-46324983 MsG0580026846.01.T01:three_prime_UTR 40.0%
TTGTTGTGTGATGCAGGAAA+TGG + Chr5:46324646-46324665 MsG0580026846.01.T01:intron 40.0%
! AAGCCTCATACAAAGAGTAC+AGG + Chr5:46324873-46324892 MsG0580026846.01.T01:three_prime_UTR 40.0%
! GTACTGGTATCAAAAGGAAG+AGG + Chr5:46324381-46324400 MsG0580026846.01.T01:CDS 40.0%
! GTTTTGTGAAGGAGGTTGAA+GGG + Chr5:46324312-46324331 MsG0580026846.01.T01:CDS 40.0%
! TTTTGTGAAGGAGGTTGAAG+GGG + Chr5:46324313-46324332 MsG0580026846.01.T01:CDS 40.0%
!! AGTTTTGTGAAGGAGGTTGA+AGG + Chr5:46324311-46324330 MsG0580026846.01.T01:CDS 40.0%
!! GTGCTAGTACTGGTATCAAA+AGG + Chr5:46324375-46324394 MsG0580026846.01.T01:CDS 40.0%
!! GTTTGTGAGTTTTGTGAAGG+AGG + Chr5:46324304-46324323 MsG0580026846.01.T01:CDS 40.0%
!! TGGGTTTGTGAGTTTTGTGA+AGG + Chr5:46324124-46324143 MsG0580026846.01.T01:CDS 40.0%
!!! CTTCTGACATGGTTGGTTTT+TGG - Chr5:46323746-46323765 None:intergenic 40.0%
!!! GAGTGGTTTTGTCAAAGCTT+CGG + Chr5:46324265-46324284 MsG0580026846.01.T01:CDS 40.0%
AAGCTGCTCAATTTCCTGTG+AGG - Chr5:46324527-46324546 None:intergenic 45.0%
CCTCGATGAAAGCAGTTTAG+AGG + Chr5:46324004-46324023 MsG0580026846.01.T01:CDS 45.0%
CCTCTAAACTGCTTTCATCG+AGG - Chr5:46324007-46324026 None:intergenic 45.0%
CTGAAGCACACTGCTTTCAT+CGG + Chr5:46325027-46325046 MsG0580026846.01.T01:three_prime_UTR 45.0%
GACAAACGAGGTTGCTATGT+TGG + Chr5:46324673-46324692 MsG0580026846.01.T01:CDS 45.0%
GAGATTTCTTCGGAGAAGGA+AGG - Chr5:46323846-46323865 None:intergenic 45.0%
GAGGAAGAAGGGAAACTGAA+AGG - Chr5:46323699-46323718 None:intergenic 45.0%
GAGTACAGGTAAGGACTCAT+TGG + Chr5:46324887-46324906 MsG0580026846.01.T01:three_prime_UTR 45.0%
GTGAGAATGCTGAGGAAGAA+GGG - Chr5:46323710-46323729 None:intergenic 45.0%
GTGGTGTGATTCTTCTGACA+TGG - Chr5:46323757-46323776 None:intergenic 45.0%
GTTGAGGCTTCCGATAAAAC+AGG + Chr5:46324098-46324117 MsG0580026846.01.T01:CDS 45.0%
TTTCATCGGTGGCAAGAATC+TGG + Chr5:46325041-46325060 MsG0580026846.01.T01:three_prime_UTR 45.0%
TTTGTTCCTTCTCCGATTGG+TGG - Chr5:46323962-46323981 None:intergenic 45.0%
! CTGCTACTTGCCAATGGTAA+TGG - Chr5:46324353-46324372 None:intergenic 45.0%
! GGAAATGGGTTTGACAAACG+AGG + Chr5:46324661-46324680 MsG0580026846.01.T01:intron 45.0%
! GTCATTGACATTGAGTGCTG+CGG + Chr5:46324178-46324197 MsG0580026846.01.T01:CDS 45.0%
! GTTTGTGACATTGAGTGCTG+CGG + Chr5:46324241-46324260 MsG0580026846.01.T01:CDS 45.0%
!!! GAGTGGTTTTGTCGAAGCTT+CGG + Chr5:46324202-46324221 MsG0580026846.01.T01:CDS 45.0%
!!! TGCTGAGAGTGGTTTTGTTG+AGG + Chr5:46324082-46324101 MsG0580026846.01.T01:CDS 45.0%
AAGCACACTGCTTTCATCGG+TGG + Chr5:46325030-46325049 MsG0580026846.01.T01:three_prime_UTR 50.0%
AGTAGCAGCAGTGCTAGTAC+TGG + Chr5:46324365-46324384 MsG0580026846.01.T01:CDS 50.0%
AGTGAAGCGTGAGAATGCTG+AGG - Chr5:46323718-46323737 None:intergenic 50.0%
CGTGAGAATGCTGAGGAAGA+AGG - Chr5:46323711-46323730 None:intergenic 50.0%
CTTCGGCGAACACTGAAAGT+GGG + Chr5:46324219-46324238 MsG0580026846.01.T01:CDS 50.0%
TCAGTGGAATGCCCCATTTC+AGG - Chr5:46324050-46324069 None:intergenic 50.0%
TCTTCGGAGAAGGAAGGAGA+GGG - Chr5:46323840-46323859 None:intergenic 50.0%
TGGGGAGATTTCTTCGGAGA+AGG - Chr5:46323850-46323869 None:intergenic 50.0%
TTCTTCGGAGAAGGAAGGAG+AGG - Chr5:46323841-46323860 None:intergenic 50.0%
! AGGATCGGAGATTTTGCGGT+CGG - Chr5:46323937-46323956 None:intergenic 50.0%
! GGCAAGGATCGGAGATTTTG+CGG - Chr5:46323941-46323960 None:intergenic 50.0%
!! TGGTGGTGGGGAGATTTCTT+CGG - Chr5:46323856-46323875 None:intergenic 50.0%
ACATCACTAGGTGGCCTCAC+AGG + Chr5:46324510-46324529 MsG0580026846.01.T01:CDS 55.0%
CTCCGATCCTTGCCACCAAT+CGG + Chr5:46323947-46323966 MsG0580026846.01.T01:CDS 55.0%
GCAGGGAGCTCAACATCACT+AGG + Chr5:46324498-46324517 MsG0580026846.01.T01:CDS 55.0%
GCTTCGGCGAACACTGAAAG+TGG + Chr5:46324218-46324237 MsG0580026846.01.T01:CDS 55.0%
GGGAGCTCAACATCACTAGG+TGG + Chr5:46324501-46324520 MsG0580026846.01.T01:CDS 55.0%
TCCTTGCCACCAATCGGAGA+AGG + Chr5:46323953-46323972 MsG0580026846.01.T01:CDS 55.0%
TTTGCGGTCGGGAACGTGTT+GGG - Chr5:46323925-46323944 None:intergenic 55.0%
! ACATTGAGTGCTGCGGAGAG+TGG + Chr5:46324185-46324204 MsG0580026846.01.T01:CDS 55.0%
! AGGATTGGACGGTAGGTTGG+TGG - Chr5:46323873-46323892 None:intergenic 55.0%
! CTCCGATTGGTGGCAAGGAT+CGG - Chr5:46323952-46323971 None:intergenic 55.0%
! GCACTGCTGCTACTTGCCAA+TGG - Chr5:46324359-46324378 None:intergenic 55.0%
! GGATCGGAGATTTTGCGGTC+GGG - Chr5:46323936-46323955 None:intergenic 55.0%
! TCCTTCTCCGATTGGTGGCA+AGG - Chr5:46323957-46323976 None:intergenic 55.0%
! TGGGTGGTGAGTTTGGAGTG+CGG - Chr5:46323779-46323798 None:intergenic 55.0%
! TTTTGCGGTCGGGAACGTGT+TGG - Chr5:46323926-46323945 None:intergenic 55.0%
!! ATTGGACGGTAGGTTGGTGG+TGG - Chr5:46323870-46323889 None:intergenic 55.0%
!! TTGGACGGTAGGTTGGTGGT+GGG - Chr5:46323869-46323888 None:intergenic 55.0%
AACTCACCACCCACCACCGT+CGG + Chr5:46323786-46323805 MsG0580026846.01.T01:CDS 60.0%
ACGTGTTGGGCTTGTGGGTC+CGG - Chr5:46323912-46323931 None:intergenic 60.0%
CGGGAACGTGTTGGGCTTGT+GGG - Chr5:46323917-46323936 None:intergenic 60.0%
GAGAAGGAAGGAGAGGGTGG+TGG - Chr5:46323834-46323853 None:intergenic 60.0%
GCGAGGATTGGACGGTAGGT+TGG - Chr5:46323876-46323895 None:intergenic 60.0%
TCGGAGAAGGAAGGAGAGGG+TGG - Chr5:46323837-46323856 None:intergenic 60.0%
TCGGGAACGTGTTGGGCTTG+TGG - Chr5:46323918-46323937 None:intergenic 60.0%
! AAGGAAGGAGAGGGTGGTGG+TGG - Chr5:46323831-46323850 None:intergenic 60.0%
! GTGGTGAGTTTGGAGTGCGG+TGG - Chr5:46323776-46323795 None:intergenic 60.0%
! GTGGTGGTGCGAGGGTTGTT+GGG - Chr5:46323812-46323831 None:intergenic 60.0%
!! ACGGTGGTGGGTGGTGAGTT+TGG - Chr5:46323786-46323805 None:intergenic 60.0%
!! TGGACGGTAGGTTGGTGGTG+GGG - Chr5:46323868-46323887 None:intergenic 60.0%
GGGAGCGAGGATTGGACGGT+AGG - Chr5:46323880-46323899 None:intergenic 65.0%
GTTGGGCTTGTGGGTCCGGT+GGG - Chr5:46323908-46323927 None:intergenic 65.0%
GTTGTTGGGCCGACGGTGGT+GGG - Chr5:46323798-46323817 None:intergenic 65.0%
TCGCGGGAGCGAGGATTGGA+CGG - Chr5:46323884-46323903 None:intergenic 65.0%
TGCGAGGGTTGTTGGGCCGA+CGG - Chr5:46323805-46323824 None:intergenic 65.0%
TGTTGGGCTTGTGGGTCCGG+TGG - Chr5:46323909-46323928 None:intergenic 65.0%
TTGTGGGTCCGGTGGGATCG+CGG - Chr5:46323901-46323920 None:intergenic 65.0%
! GGTGGTGGTGCGAGGGTTGT+TGG - Chr5:46323813-46323832 None:intergenic 65.0%
!! GAAGGAGAGGGTGGTGGTGG+TGG - Chr5:46323828-46323847 None:intergenic 65.0%
GAGGGTTGTTGGGCCGACGG+TGG - Chr5:46323802-46323821 None:intergenic 70.0%
GGGATCGCGGGAGCGAGGAT+TGG - Chr5:46323888-46323907 None:intergenic 70.0%
GGTTGTTGGGCCGACGGTGG+TGG - Chr5:46323799-46323818 None:intergenic 70.0%
TGTGGGTCCGGTGGGATCGC+GGG - Chr5:46323900-46323919 None:intergenic 70.0%
!! AGGGTGGTGGTGGTGGTGCG+AGG - Chr5:46323821-46323840 None:intergenic 70.0%
!! GGGTGGTGGTGGTGGTGCGA+GGG - Chr5:46323820-46323839 None:intergenic 70.0%
CCTCGCTCCCGCGATCCCAC+CGG + Chr5:46323890-46323909 MsG0580026846.01.T01:CDS 75.0%
!! GTTGGGCCGACGGTGGTGGG+TGG - Chr5:46323795-46323814 None:intergenic 75.0%
! CCGGTGGGATCGCGGGAGCG+AGG - Chr5:46323893-46323912 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr5 gene 46323697 46325230 46323697 ID=MsG0580026846.01;Name=MsG0580026846.01
Chr5 mRNA 46323697 46325230 46323697 ID=MsG0580026846.01.T01;Parent=MsG0580026846.01;Name=MsG0580026846.01.T01;_AED=0.42;_eAED=0.42;_QI=59|1|1|1|1|1|2|410|324
Chr5 exon 46323697 46324571 46323697 ID=MsG0580026846.01.T01:exon:1373;Parent=MsG0580026846.01.T01
Chr5 exon 46324662 46325230 46324662 ID=MsG0580026846.01.T01:exon:1374;Parent=MsG0580026846.01.T01
Chr5 five_prime_UTR 46323697 46323755 46323697 ID=MsG0580026846.01.T01:five_prime_utr;Parent=MsG0580026846.01.T01
Chr5 CDS 46323756 46324571 46323756 ID=MsG0580026846.01.T01:cds;Parent=MsG0580026846.01.T01
Chr5 CDS 46324662 46324820 46324662 ID=MsG0580026846.01.T01:cds;Parent=MsG0580026846.01.T01
Chr5 three_prime_UTR 46324821 46325230 46324821 ID=MsG0580026846.01.T01:three_prime_utr;Parent=MsG0580026846.01.T01
Gene Sequence

>MsG0580026846.01.T01

ATGTCAGAAGAATCACACCACCGCACTCCAAACTCACCACCCACCACCGTCGGCCCAACAACCCTCGCACCACCACCACCACCCTCTCCTTCCTTCTCCGAAGAAATCTCCCCACCACCAACCTACCGTCCAATCCTCGCTCCCGCGATCCCACCGGACCCACAAGCCCAACACGTTCCCGACCGCAAAATCTCCGATCCTTGCCACCAATCGGAGAAGGAACAAAAAATAGGGCAAAATCTCCCAAACCTCGATGAAAGCAGTTTAGAGGTCGATCAAAAGCTTGTTTTACCTGAAATGGGGCATTCCACTGACAAAGATAGTATTGCTGAGAGTGGTTTTGTTGAGGCTTCCGATAAAACAGGAAATGGGTTTGTGAGTTTTGTGAAGAAAGAAGATAGATGTAAGAACAAAGAGCCATTGTCATTGACATTGAGTGCTGCGGAGAGTGGTTTTGTCGAAGCTTCGGCGAACACTGAAAGTGGGTTTGTGACATTGAGTGCTGCGGAGAGTGGTTTTGTCAAAGCTTCGGCGAACACTGAAAGTGGGTTTGTGAGTTTTGTGAAGGAGGTTGAAGGGGAAGATCAAGAATTGCCATTACCATTGGCAAGTAGCAGCAGTGCTAGTACTGGTATCAAAAGGAAGAGGTTGCAATTTGATGATATAAGGAAACATTTTGATGTTCCTATAAACGTGGCTGCTAAGAAAATGAAAGTTGGCGTGACTCTTTTGAAAAATAGATGCAGGGAGCTCAACATCACTAGGTGGCCTCACAGGAAATTGAGCAGCTTGACTGTACTCATCAACAACCGTAAGGAAATGGGTTTGACAAACGAGGTTGCTATGTTGGAGAAGAAGAAAAAGATGTTAGAGAAATTTCCTAAAACGGAATTGAATGAAAAAATCAAGAAGCTTAGGCAAGCATGTTTTAAAGCTACATACAAGAAGAGAAGATTAGTTACCCTCAACCCTTGA
Protein sequence

>MsG0580026846.01.T01

MSEESHHRTPNSPPTTVGPTTLAPPPPPSPSFSEEISPPPTYRPILAPAIPPDPQAQHVPDRKISDPCHQSEKEQKIGQNLPNLDESSLEVDQKLVLPEMGHSTDKDSIAESGFVEASDKTGNGFVSFVKKEDRCKNKEPLSLTLSAAESGFVEASANTESGFVTLSAAESGFVKASANTESGFVSFVKEVEGEDQELPLPLASSSSASTGIKRKRLQFDDIRKHFDVPINVAAKKMKVGVTLLKNRCRELNITRWPHRKLSSLTVLINNRKEMGLTNEVAMLEKKKKMLEKFPKTELNEKIKKLRQACFKATYKKRRLVTLNP*