AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027342.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027342.01.T01 MTR_5g055100 92.135 89 7 0 1 89 1 89 3.45e-56 173
MsG0580027342.01.T01 MTR_5g045560 91.011 89 8 0 1 89 1 89 8.90e-56 172
MsG0580027342.01.T01 MTR_1g077360 88.764 89 10 0 1 89 1 89 1.69e-54 169
MsG0580027342.01.T01 MTR_5g053390 87.640 89 11 0 1 89 1 89 1.10e-53 167
MsG0580027342.01.T01 MTR_1g075600 86.517 89 12 0 1 89 1 89 7.26e-53 165
MsG0580027342.01.T01 MTR_2g085250 86.517 89 12 0 1 89 1 89 1.11e-52 164
MsG0580027342.01.T01 MTR_2g085280 86.517 89 12 0 1 89 1 89 1.19e-52 164
MsG0580027342.01.T01 MTR_3g052870 82.022 89 16 0 1 89 1 89 5.53e-52 162
MsG0580027342.01.T01 MTR_1g105920 82.022 89 16 0 1 89 1 89 1.12e-51 162
MsG0580027342.01.T01 MTR_2g030740 80.899 89 17 0 1 89 1 89 5.37e-51 160
MsG0580027342.01.T01 MTR_1g105910 80.899 89 17 0 1 89 1 89 2.44e-50 158
MsG0580027342.01.T01 MTR_1g106070 77.528 89 20 0 1 89 1 89 2.09e-49 154
MsG0580027342.01.T01 MTR_1g012570 80.899 89 17 0 1 89 1 89 4.61e-49 155
MsG0580027342.01.T01 MTR_1g108510 80.899 89 17 0 1 89 1 89 5.97e-49 154
MsG0580027342.01.T01 MTR_3g030770 80.899 89 17 0 1 89 1 89 6.10e-49 154
MsG0580027342.01.T01 MTR_8g043650 78.652 89 19 0 1 89 1 89 1.51e-48 154
MsG0580027342.01.T01 MTR_3g052920 78.652 89 19 0 1 89 1 89 1.69e-48 153
MsG0580027342.01.T01 MTR_2g093190 77.528 89 20 0 1 89 1 89 3.33e-46 147
MsG0580027342.01.T01 MTR_3g030780 71.910 89 25 0 1 89 1 89 1.71e-45 142
MsG0580027342.01.T01 MTR_1g108580 73.034 89 24 0 1 89 1 89 5.06e-45 144
MsG0580027342.01.T01 MTR_0121s0100 73.034 89 24 0 1 89 1 89 1.61e-43 137
MsG0580027342.01.T01 MTR_0121s0080 71.910 89 25 0 1 89 1 89 4.27e-43 140
MsG0580027342.01.T01 MTR_1g105905 80.556 72 14 0 18 89 1 72 5.00e-39 129
MsG0580027342.01.T01 MTR_3g080940 72.000 75 21 0 9 83 9 83 2.29e-35 119
MsG0580027342.01.T01 MTR_3g067910 78.261 69 15 0 21 89 2 70 4.02e-35 115
MsG0580027342.01.T01 MTR_3g067875 87.500 56 7 0 18 73 1 56 6.07e-32 106
MsG0580027342.01.T01 MTR_7g062350 56.818 88 37 1 3 89 14 101 5.10e-30 107
MsG0580027342.01.T01 MTR_8g086290 59.459 74 30 0 5 78 8 81 1.48e-28 101
MsG0580027342.01.T01 MTR_3g067870 73.016 63 17 0 16 78 83 145 7.43e-27 96.3
MsG0580027342.01.T01 MTR_2g105290 53.425 73 34 0 5 77 8 80 1.18e-25 94.0
MsG0580027342.01.T01 MTR_7g028448 56.790 81 35 0 5 85 8 88 1.22e-25 93.6
MsG0580027342.01.T01 MTR_4g036915 56.790 81 35 0 5 85 8 88 1.22e-25 93.6
MsG0580027342.01.T01 MTR_1g047550 50.649 77 38 0 9 85 2 78 3.00e-25 92.8
MsG0580027342.01.T01 MTR_8g079502 53.521 71 33 0 9 79 2 72 4.95e-25 92.4
MsG0580027342.01.T01 MTR_4g131030 49.367 79 40 0 9 87 2 80 9.48e-25 92.4
MsG0580027342.01.T01 MTR_1g108500 80.000 50 10 0 22 71 110 159 2.68e-24 93.6
MsG0580027342.01.T01 MTR_1g075570 63.889 72 12 1 18 89 1 58 7.66e-24 89.0
MsG0580027342.01.T01 MTR_8g051580 49.367 79 40 0 9 87 2 80 2.53e-23 88.2
MsG0580027342.01.T01 MTR_8g046350 51.948 77 37 0 9 85 2 78 3.37e-23 87.4
MsG0580027342.01.T01 MTR_1g054265 46.835 79 42 0 9 87 2 80 4.53e-23 87.0
MsG0580027342.01.T01 MTR_1g063160 49.315 73 37 0 9 81 2 74 9.51e-23 86.7
MsG0580027342.01.T01 MTR_4g127140 57.534 73 31 0 5 77 8 80 7.25e-21 81.6
MsG0580027342.01.T01 MTR_4g094638 52.174 69 33 0 9 77 2 70 3.13e-20 80.1
MsG0580027342.01.T01 MTR_3g005530 51.471 68 33 0 9 76 2 69 6.47e-20 80.1
MsG0580027342.01.T01 MTR_4g094632 52.174 69 33 0 9 77 2 70 6.78e-20 78.6
MsG0580027342.01.T01 MTR_4g093970 56.061 66 29 0 10 75 29 94 1.35e-19 79.7
MsG0580027342.01.T01 MTR_4g102530 50.000 72 36 0 10 81 3 74 4.41e-19 77.8
MsG0580027342.01.T01 MTR_3g452380 47.297 74 39 0 3 76 11 84 6.20e-19 78.2
MsG0580027342.01.T01 MTR_8g033270 50.633 79 37 1 9 85 2 80 6.39e-19 78.2
MsG0580027342.01.T01 MTR_4g109830 52.174 69 32 1 9 76 2 70 1.64e-18 76.6
MsG0580027342.01.T01 MTR_1g053070 54.412 68 31 0 9 76 2 69 1.70e-18 77.0
MsG0580027342.01.T01 MTR_1g101970 52.941 68 32 0 9 76 2 69 5.87e-18 74.3
MsG0580027342.01.T01 MTR_5g066180 49.296 71 36 0 9 79 2 72 5.92e-18 75.1
MsG0580027342.01.T01 MTR_8g087860 50.000 68 34 0 9 76 18 85 6.07e-18 75.5
MsG0580027342.01.T01 MTR_7g075870 48.148 81 38 1 10 86 3 83 6.48e-18 74.7
MsG0580027342.01.T01 MTR_8g033220 46.667 75 40 0 10 84 3 77 8.97e-18 74.7
MsG0580027342.01.T01 MTR_8g033220 46.667 75 40 0 10 84 3 77 9.27e-18 74.7
MsG0580027342.01.T01 MTR_0003s0590 52.941 68 32 0 9 76 2 69 1.14e-17 74.7
MsG0580027342.01.T01 MTR_6g464720 52.174 69 32 1 9 76 2 70 1.29e-17 74.7
MsG0580027342.01.T01 MTR_2g017865 50.000 68 34 0 9 76 17 84 1.47e-17 74.7
MsG0580027342.01.T01 MTR_6g015975 50.725 69 33 1 9 76 2 70 4.57e-17 73.2
MsG0580027342.01.T01 MTR_5g032150 50.000 68 34 0 9 76 2 69 4.65e-17 72.8
MsG0580027342.01.T01 MTR_5g032520 50.000 68 34 0 9 76 2 69 5.14e-17 72.8
MsG0580027342.01.T01 MTR_2g009890 43.421 76 43 0 9 84 2 77 5.16e-17 73.6
MsG0580027342.01.T01 MTR_7g016600 50.725 69 33 1 9 76 2 70 6.64e-17 72.8
MsG0580027342.01.T01 MTR_7g016630 45.833 72 38 1 9 79 2 73 7.64e-17 72.8
MsG0580027342.01.T01 MTR_8g066260 47.826 69 35 1 9 76 2 70 9.73e-17 72.0
MsG0580027342.01.T01 MTR_7g075870 47.561 82 38 2 10 86 3 84 1.02e-16 71.6
MsG0580027342.01.T01 MTR_3g102570 49.296 71 35 1 9 78 2 72 1.03e-16 73.2
MsG0580027342.01.T01 MTR_5g021270 51.429 70 32 1 9 76 2 71 1.15e-16 71.6
MsG0580027342.01.T01 MTR_5g021270 51.429 70 32 1 9 76 2 71 1.20e-16 71.6
MsG0580027342.01.T01 MTR_5g031000 45.588 68 37 0 9 76 2 69 1.78e-16 71.2
MsG0580027342.01.T01 MTR_5g031000 45.588 68 37 0 9 76 2 69 2.00e-16 71.2
MsG0580027342.01.T01 MTR_4g084740 48.101 79 39 2 9 86 2 79 2.40e-16 72.0
MsG0580027342.01.T01 MTR_5g046790 47.826 69 35 1 9 76 2 70 2.74e-16 70.5
MsG0580027342.01.T01 MTR_2g461710 53.571 56 26 0 9 64 2 57 3.24e-16 66.6
MsG0580027342.01.T01 MTR_3g088615 45.714 70 36 1 9 76 2 71 4.77e-16 69.3
MsG0580027342.01.T01 MTR_7g075850 46.341 82 41 2 9 87 2 83 4.97e-16 70.5
MsG0580027342.01.T01 MTR_5g046870 49.275 69 34 1 9 76 2 70 5.86e-16 67.4
MsG0580027342.01.T01 MTR_1g038300 46.429 84 39 2 9 86 2 85 6.12e-16 70.1
MsG0580027342.01.T01 MTR_1g029670 48.485 66 33 1 9 74 2 66 1.16e-15 68.2
MsG0580027342.01.T01 MTR_5g066960 49.123 57 29 0 9 65 2 58 2.44e-15 64.3
MsG0580027342.01.T01 MTR_4g109810 53.571 56 26 0 9 64 2 57 4.10e-15 67.8
MsG0580027342.01.T01 MTR_8g097090 55.357 56 25 0 9 64 2 57 4.45e-15 67.8
MsG0580027342.01.T01 MTR_3g084980 53.571 56 26 0 9 64 2 57 4.85e-15 67.8
MsG0580027342.01.T01 MTR_3g113030 45.714 70 36 1 9 76 2 71 5.51e-15 67.4
MsG0580027342.01.T01 MTR_4g093030 46.774 62 33 0 9 70 2 63 1.43e-13 62.4
MsG0580027342.01.T01 MTR_4g051538 38.202 89 55 0 1 89 1 89 1.59e-13 63.5
MsG0580027342.01.T01 MTR_3g067915 50.877 57 28 0 33 89 3 59 2.89e-13 60.1
MsG0580027342.01.T01 MTR_4g036050 41.429 70 40 1 9 77 2 71 4.67e-13 61.2
MsG0580027342.01.T01 MTR_4g036050 41.429 70 40 1 9 77 2 71 4.98e-13 61.6
MsG0580027342.01.T01 MTR_4g036050 41.429 70 40 1 9 77 2 71 5.25e-13 61.6
MsG0580027342.01.T01 MTR_4g036050 41.429 70 40 1 9 77 2 71 5.61e-13 61.6
MsG0580027342.01.T01 MTR_8g022970 42.857 70 40 0 10 79 19 88 2.57e-12 59.7
MsG0580027342.01.T01 MTR_4g108720 41.558 77 43 1 9 83 2 78 6.97e-12 59.7
MsG0580027342.01.T01 MTR_3g437790 40.278 72 35 2 26 89 30 101 1.68e-11 57.4
MsG0580027342.01.T01 MTR_1g041615 56.522 46 20 0 20 65 17 62 1.97e-11 56.2
MsG0580027342.01.T02 MTR_5g045560 92.000 75 6 0 1 75 15 89 7.38e-47 149
MsG0580027342.01.T02 MTR_5g055100 92.000 75 6 0 1 75 15 89 3.57e-46 147
MsG0580027342.01.T02 MTR_1g077360 89.333 75 8 0 1 75 15 89 4.08e-45 144
MsG0580027342.01.T02 MTR_2g085250 89.333 75 8 0 1 75 15 89 5.13e-45 144
MsG0580027342.01.T02 MTR_2g085280 89.333 75 8 0 1 75 15 89 5.60e-45 144
MsG0580027342.01.T02 MTR_5g053390 88.000 75 9 0 1 75 15 89 2.27e-44 142
MsG0580027342.01.T02 MTR_1g075600 86.667 75 10 0 1 75 15 89 7.68e-44 141
MsG0580027342.01.T02 MTR_3g052870 84.000 75 12 0 1 75 15 89 1.04e-43 140
MsG0580027342.01.T02 MTR_1g105920 84.000 75 12 0 1 75 15 89 2.28e-43 140
MsG0580027342.01.T02 MTR_1g105910 82.667 75 13 0 1 75 15 89 3.71e-42 137
MsG0580027342.01.T02 MTR_2g030740 81.333 75 14 0 1 75 15 89 7.04e-42 136
MsG0580027342.01.T02 MTR_8g043650 81.333 75 14 0 1 75 15 89 8.20e-42 136
MsG0580027342.01.T02 MTR_1g106070 78.667 75 16 0 1 75 15 89 2.98e-41 133
MsG0580027342.01.T02 MTR_1g012570 82.667 75 13 0 1 75 15 89 8.27e-41 133
MsG0580027342.01.T02 MTR_1g108510 81.333 75 14 0 1 75 15 89 1.12e-40 133
MsG0580027342.01.T02 MTR_3g030770 81.333 75 14 0 1 75 15 89 1.17e-40 132
MsG0580027342.01.T02 MTR_3g052920 80.000 75 15 0 1 75 15 89 3.81e-40 132
MsG0580027342.01.T02 MTR_1g105905 80.556 72 14 0 4 75 1 72 3.90e-39 129
MsG0580027342.01.T02 MTR_2g093190 78.667 75 16 0 1 75 15 89 7.42e-39 128
MsG0580027342.01.T02 MTR_1g108580 74.667 75 19 0 1 75 15 89 1.85e-38 127
MsG0580027342.01.T02 MTR_0121s0080 74.667 75 19 0 1 75 15 89 1.18e-37 125
MsG0580027342.01.T02 MTR_3g030780 72.000 75 21 0 1 75 15 89 3.69e-37 121
MsG0580027342.01.T02 MTR_0121s0100 72.000 75 21 0 1 75 15 89 3.24e-35 116
MsG0580027342.01.T02 MTR_3g067910 78.261 69 15 0 7 75 2 70 5.17e-35 115
MsG0580027342.01.T02 MTR_3g080940 68.000 75 24 0 1 75 15 89 1.92e-32 111
MsG0580027342.01.T02 MTR_3g067875 87.500 56 7 0 4 59 1 56 5.18e-32 106
MsG0580027342.01.T02 MTR_7g062350 62.667 75 27 1 2 75 27 101 1.84e-29 104
MsG0580027342.01.T02 MTR_3g067870 73.016 63 17 0 2 64 83 145 5.46e-27 96.3
MsG0580027342.01.T02 MTR_7g028448 61.017 59 23 0 8 66 25 83 2.79e-25 92.0
MsG0580027342.01.T02 MTR_4g036915 61.017 59 23 0 8 66 25 83 2.79e-25 92.0
MsG0580027342.01.T02 MTR_1g108500 80.000 50 10 0 8 57 110 159 2.84e-24 92.8
MsG0580027342.01.T02 MTR_1g075570 63.889 72 12 1 4 75 1 58 9.64e-24 88.2
MsG0580027342.01.T02 MTR_8g086290 57.812 64 27 0 1 64 18 81 2.16e-23 87.4
MsG0580027342.01.T02 MTR_1g047550 55.738 61 27 0 11 71 18 78 8.25e-22 83.6
MsG0580027342.01.T02 MTR_8g079502 60.000 55 22 0 11 65 18 72 2.53e-21 82.4
MsG0580027342.01.T02 MTR_4g131030 53.968 63 29 0 11 73 18 80 3.04e-21 82.8
MsG0580027342.01.T02 MTR_8g051580 55.556 63 28 0 11 73 18 80 3.25e-21 82.0
MsG0580027342.01.T02 MTR_8g046350 57.377 61 26 0 11 71 18 78 1.45e-20 79.7
MsG0580027342.01.T02 MTR_2g105290 50.794 63 31 0 1 63 18 80 1.55e-20 80.1
MsG0580027342.01.T02 MTR_1g054265 45.205 73 40 0 1 73 8 80 7.53e-20 78.2
MsG0580027342.01.T02 MTR_4g127140 55.357 56 25 0 8 63 25 80 1.59e-19 77.8
MsG0580027342.01.T02 MTR_1g063160 47.761 67 35 0 1 67 8 74 2.21e-19 77.4
MsG0580027342.01.T02 MTR_4g102530 50.746 67 33 0 1 67 8 74 2.19e-18 75.1
MsG0580027342.01.T02 MTR_4g093970 54.098 61 28 0 1 61 34 94 6.05e-17 72.0
MsG0580027342.01.T02 MTR_7g075870 48.684 76 35 1 1 72 8 83 7.15e-17 71.2
MsG0580027342.01.T02 MTR_8g033220 47.143 70 37 0 1 70 8 77 1.06e-16 71.2
MsG0580027342.01.T02 MTR_8g033220 47.143 70 37 0 1 70 8 77 1.16e-16 71.2
MsG0580027342.01.T02 MTR_3g005530 48.387 62 32 0 1 62 8 69 1.44e-16 70.9
MsG0580027342.01.T02 MTR_4g109830 53.968 63 28 1 1 62 8 70 2.36e-16 70.5
MsG0580027342.01.T02 MTR_1g101970 53.226 62 29 0 1 62 8 69 3.35e-16 69.3
MsG0580027342.01.T02 MTR_8g033270 50.685 73 34 1 1 71 8 80 3.52e-16 70.1
MsG0580027342.01.T02 MTR_5g066180 49.231 65 33 0 1 65 8 72 4.81e-16 69.3
MsG0580027342.01.T02 MTR_4g094638 47.619 63 33 0 1 63 8 70 7.33e-16 68.2
MsG0580027342.01.T02 MTR_4g094632 50.000 60 30 0 4 63 11 70 7.63e-16 67.8
MsG0580027342.01.T02 MTR_7g075870 48.052 77 35 2 1 72 8 84 9.58e-16 68.6
MsG0580027342.01.T02 MTR_6g464720 53.968 63 28 1 1 62 8 70 1.98e-15 68.2
MsG0580027342.01.T02 MTR_1g053070 51.613 62 30 0 1 62 8 69 2.70e-15 67.8
MsG0580027342.01.T02 MTR_2g009890 41.429 70 41 0 1 70 8 77 5.13e-15 67.4
MsG0580027342.01.T02 MTR_5g032150 50.000 62 31 0 1 62 8 69 5.89e-15 66.6
MsG0580027342.01.T02 MTR_5g032520 50.000 62 31 0 1 62 8 69 7.55e-15 66.2
MsG0580027342.01.T02 MTR_3g452380 45.161 62 34 0 1 62 23 84 1.06e-14 66.2
MsG0580027342.01.T02 MTR_7g016630 46.970 66 34 1 1 65 8 73 1.06e-14 66.2
MsG0580027342.01.T02 MTR_0003s0590 50.000 62 31 0 1 62 8 69 1.34e-14 65.9
MsG0580027342.01.T02 MTR_8g087860 46.774 62 33 0 1 62 24 85 1.45e-14 65.9
MsG0580027342.01.T02 MTR_3g102570 49.231 65 32 1 1 64 8 72 1.50e-14 66.6
MsG0580027342.01.T02 MTR_5g031000 43.548 62 35 0 1 62 8 69 1.63e-14 65.5
MsG0580027342.01.T02 MTR_5g031000 43.548 62 35 0 1 62 8 69 1.81e-14 65.5
MsG0580027342.01.T02 MTR_6g015975 50.794 63 30 1 1 62 8 70 2.95e-14 65.1
MsG0580027342.01.T02 MTR_2g017865 46.774 62 33 0 1 62 23 84 3.87e-14 64.7
MsG0580027342.01.T02 MTR_7g016600 50.794 63 30 1 1 62 8 70 4.29e-14 64.7
MsG0580027342.01.T02 MTR_2g461710 56.000 50 22 0 1 50 8 57 5.06e-14 60.5
MsG0580027342.01.T02 MTR_4g084740 46.575 73 37 2 1 72 8 79 9.31e-14 64.3
MsG0580027342.01.T02 MTR_5g046790 47.619 63 32 1 1 62 8 70 1.04e-13 63.2
MsG0580027342.01.T02 MTR_8g066260 49.206 63 31 1 1 62 8 70 1.08e-13 63.5
MsG0580027342.01.T02 MTR_3g067915 50.877 57 28 0 19 75 3 59 1.28e-13 60.5
MsG0580027342.01.T02 MTR_7g075850 46.053 76 38 2 1 73 8 83 2.10e-13 62.8
MsG0580027342.01.T02 MTR_4g051538 40.299 67 40 0 9 75 23 89 3.00e-13 62.0
MsG0580027342.01.T02 MTR_5g021270 48.438 64 31 1 1 62 8 71 3.47e-13 62.0
MsG0580027342.01.T02 MTR_5g021270 48.438 64 31 1 1 62 8 71 3.62e-13 62.0
MsG0580027342.01.T02 MTR_3g113030 45.312 64 33 1 1 62 8 71 4.10e-13 61.6
MsG0580027342.01.T02 MTR_1g038300 43.590 78 38 2 1 72 8 85 4.41e-13 61.6
MsG0580027342.01.T02 MTR_5g046870 47.619 63 32 1 1 62 8 70 5.88e-13 59.3
MsG0580027342.01.T02 MTR_3g088615 42.188 64 35 1 1 62 8 71 8.64e-13 60.1
MsG0580027342.01.T02 MTR_1g029670 45.000 60 32 1 1 60 8 66 1.78e-12 59.3
MsG0580027342.01.T02 MTR_5g066960 48.000 50 26 0 1 50 8 57 1.85e-12 56.6
MsG0580027342.01.T02 MTR_4g109810 54.000 50 23 0 1 50 8 57 2.43e-12 59.7
MsG0580027342.01.T02 MTR_8g097090 56.000 50 22 0 1 50 8 57 2.66e-12 59.7
MsG0580027342.01.T02 MTR_3g084980 54.000 50 23 0 1 50 8 57 8.71e-12 58.2
MsG0580027342.01.T02 MTR_1g041615 56.522 46 20 0 6 51 17 62 1.01e-11 56.6
MsG0580027342.01.T02 MTR_8g022970 49.057 53 27 0 13 65 36 88 1.32e-11 57.0
MsG0580027342.01.T02 MTR_4g036050 42.188 64 36 1 1 63 8 71 1.55e-11 56.6
MsG0580027342.01.T02 MTR_3g437790 40.278 72 35 2 12 75 30 101 1.55e-11 57.0
MsG0580027342.01.T02 MTR_4g036050 42.188 64 36 1 1 63 8 71 1.65e-11 57.0
MsG0580027342.01.T02 MTR_4g036050 42.188 64 36 1 1 63 8 71 1.91e-11 56.6
MsG0580027342.01.T02 MTR_4g093030 44.643 56 31 0 1 56 8 63 1.92e-11 56.2
MsG0580027342.01.T02 MTR_3g109930 47.170 53 28 0 13 65 20 72 2.35e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027342.01.T01 AT5G60440 74.026 77 20 0 5 81 3 79 8.14e-39 130
MsG0580027342.01.T01 AT1G65360 65.432 81 28 0 5 85 3 83 2.23e-34 117
MsG0580027342.01.T01 AT1G01530 64.634 82 29 0 4 85 2 83 5.17e-34 117
MsG0580027342.01.T01 AT4G36590 67.059 85 26 1 5 89 3 85 6.70e-34 117
MsG0580027342.01.T01 AT2G24840 70.423 71 21 0 9 79 63 133 5.25e-32 112
MsG0580027342.01.T01 AT2G34440 50.704 71 35 0 9 79 2 72 3.45e-23 87.4
MsG0580027342.01.T01 AT1G72350 57.534 73 29 1 9 81 43 113 3.89e-23 88.6
MsG0580027342.01.T01 AT3G66656 55.882 68 30 0 9 76 2 69 5.86e-23 87.0
MsG0580027342.01.T01 AT3G04100 42.045 88 48 1 2 86 7 94 1.53e-21 84.0
MsG0580027342.01.T01 AT2G45660 54.412 68 31 0 10 77 3 70 1.31e-20 80.5
MsG0580027342.01.T01 AT2G45650 55.882 68 30 0 9 76 2 69 1.37e-20 82.4
MsG0580027342.01.T01 AT5G51870 54.167 72 33 0 5 76 21 92 3.58e-20 80.5
MsG0580027342.01.T01 AT2G45660 54.412 68 31 0 10 77 3 70 3.60e-20 80.9
MsG0580027342.01.T01 AT4G09960 50.000 68 34 0 9 76 2 69 1.23e-19 79.3
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MsG0580027342.01.T01 AT5G65070 49.123 57 29 0 9 65 2 58 1.32e-13 63.9
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MsG0580027342.01.T01 AT1G18750 37.662 77 46 1 9 83 2 78 9.20e-13 62.4
MsG0580027342.01.T01 AT1G18750 37.662 77 46 1 9 83 2 78 9.36e-13 62.4
MsG0580027342.01.T01 AT1G18750 37.662 77 46 1 9 83 2 78 1.01e-12 62.4
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MsG0580027342.01.T01 AT2G26320 41.892 74 41 1 5 76 14 87 1.56e-12 58.5
MsG0580027342.01.T01 AT5G49490 38.636 88 47 2 5 85 41 128 2.63e-12 60.8
MsG0580027342.01.T01 AT5G49420 36.782 87 50 2 5 86 3 89 5.65e-12 60.1
MsG0580027342.01.T01 AT1G46408 41.892 74 37 2 10 78 5 77 9.97e-12 58.9
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MsG0580027342.01.T01 AT1G77080 46.377 69 36 1 9 76 2 70 2.20e-11 57.0
MsG0580027342.01.T01 AT1G77080 46.377 69 36 1 9 76 2 70 3.95e-11 56.6
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MsG0580027342.01.T02 AT1G65360 66.667 69 23 0 3 71 15 83 5.91e-29 103
MsG0580027342.01.T02 AT4G36590 68.000 75 22 1 1 75 13 85 6.98e-29 103
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MsG0580027342.01.T02 AT2G45650 56.452 62 27 0 1 62 8 69 6.10e-18 75.1
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MsG0580027342.01.T02 AT4G22950 48.485 66 34 0 1 66 8 73 2.64e-17 72.8
MsG0580027342.01.T02 AT4G22950 48.485 66 34 0 1 66 8 73 2.64e-17 72.8
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MsG0580027342.01.T02 AT5G51870 53.226 62 29 0 1 62 8 69 4.33e-17 72.0
MsG0580027342.01.T02 AT5G51870 53.226 62 29 0 1 62 31 92 6.73e-17 71.2
MsG0580027342.01.T02 AT5G51870 53.226 62 29 0 1 62 8 69 6.77e-17 71.6
MsG0580027342.01.T02 AT5G51870 53.226 62 29 0 1 62 18 79 9.21e-17 71.6
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MsG0580027342.01.T02 AT1G17310 54.237 59 25 1 11 69 75 131 1.65e-16 70.9
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MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 1.91e-16 69.3
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 1.91e-16 69.3
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 1.91e-16 69.3
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 2.65e-16 68.9
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 2.65e-16 68.9
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 2.65e-16 68.9
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 2.65e-16 68.9
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 2.65e-16 68.9
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MsG0580027342.01.T02 AT4G09960 46.774 62 33 0 1 62 9 70 3.75e-16 69.7
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MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 6.26e-16 68.6
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MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 6.55e-16 68.9
MsG0580027342.01.T02 AT5G62165 50.000 62 31 0 1 62 8 69 6.55e-16 68.9
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MsG0580027342.01.T02 AT4G37940 46.774 62 33 0 1 62 8 69 1.64e-15 67.0
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MsG0580027342.01.T02 AT5G51860 50.000 62 31 0 1 62 8 69 2.34e-15 67.4
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MsG0580027342.01.T02 AT3G57230 45.161 62 34 0 1 62 8 69 2.34e-15 67.8
MsG0580027342.01.T02 AT2G22540 50.000 62 31 0 1 62 8 69 2.37e-15 67.8
MsG0580027342.01.T02 AT2G22540 50.000 62 31 0 1 62 8 69 2.37e-15 67.8
MsG0580027342.01.T02 AT4G11880 47.761 67 34 1 1 66 8 74 2.82e-15 66.6
MsG0580027342.01.T02 AT4G11880 47.761 67 34 1 1 66 8 74 2.82e-15 66.6
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MsG0580027342.01.T02 AT4G11880 47.761 67 34 1 1 66 8 74 4.51e-15 67.0
MsG0580027342.01.T02 AT4G11880 47.761 67 34 1 1 66 8 74 4.51e-15 67.0
MsG0580027342.01.T02 AT5G60910 50.794 63 30 1 1 62 8 70 4.92e-15 67.0
MsG0580027342.01.T02 AT3G57390 53.226 62 29 0 1 62 8 69 5.17e-15 67.4
MsG0580027342.01.T02 AT2G42830 46.774 62 33 0 1 62 23 84 1.05e-14 66.2
MsG0580027342.01.T02 AT2G42830 46.774 62 33 0 1 62 23 84 1.07e-14 66.2
MsG0580027342.01.T02 AT3G54340 48.438 64 31 1 1 62 8 71 1.12e-14 65.9
MsG0580027342.01.T02 AT3G30260 45.833 72 38 1 1 71 8 79 1.19e-14 66.2
MsG0580027342.01.T02 AT5G10140 46.970 66 34 1 1 65 8 73 1.20e-14 64.7
MsG0580027342.01.T02 AT3G58780 46.774 62 33 0 1 62 23 84 1.50e-14 65.9
MsG0580027342.01.T02 AT4G18960 46.774 62 33 0 1 62 24 85 1.52e-14 65.9
MsG0580027342.01.T02 AT2G14210 47.619 63 32 1 1 62 8 70 1.60e-14 65.5
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MsG0580027342.01.T02 AT3G58780 46.774 62 33 0 1 62 29 90 1.68e-14 65.9
MsG0580027342.01.T02 AT2G22630 46.774 62 33 0 1 62 8 69 1.84e-14 65.5
MsG0580027342.01.T02 AT2G22630 46.774 62 33 0 1 62 8 69 1.84e-14 65.5
MsG0580027342.01.T02 AT2G14210 47.619 63 32 1 1 62 8 70 1.91e-14 65.5
MsG0580027342.01.T02 AT5G10140 46.970 66 34 1 1 65 8 73 1.93e-14 64.7
MsG0580027342.01.T02 AT1G69120 47.619 63 32 1 1 62 8 70 2.03e-14 65.5
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MsG0580027342.01.T02 AT5G13790 46.774 62 33 0 1 62 8 69 2.59e-14 65.5
MsG0580027342.01.T02 AT1G77980 47.692 65 33 1 1 64 8 72 2.59e-14 65.9
MsG0580027342.01.T02 AT5G13790 46.774 62 33 0 1 62 8 69 2.74e-14 65.5
MsG0580027342.01.T02 AT4G09960 46.774 62 33 0 1 62 103 164 3.46e-14 65.5
MsG0580027342.01.T02 AT1G69120 47.619 63 32 1 1 62 8 70 4.24e-14 64.7
MsG0580027342.01.T02 AT1G22130 47.143 70 35 2 1 69 8 76 4.97e-14 65.1
MsG0580027342.01.T02 AT1G26310 46.032 63 33 1 1 62 8 70 5.73e-14 64.3
MsG0580027342.01.T02 AT5G23260 44.444 81 39 2 1 75 8 88 7.61e-14 63.9
MsG0580027342.01.T02 AT1G77950 42.857 70 38 2 1 69 8 76 8.07e-14 63.9
MsG0580027342.01.T02 AT1G77950 42.857 70 38 2 1 69 8 76 8.07e-14 63.9
MsG0580027342.01.T02 AT1G77950 42.857 70 38 2 1 69 8 76 8.07e-14 63.9
MsG0580027342.01.T02 AT1G77950 42.857 70 38 2 1 69 8 76 8.07e-14 63.9
MsG0580027342.01.T02 AT1G71692 46.875 64 33 1 1 63 8 71 1.06e-13 63.2
MsG0580027342.01.T02 AT5G23260 44.444 81 39 2 1 75 8 88 1.34e-13 63.5
MsG0580027342.01.T02 AT5G23260 44.444 81 39 2 1 75 24 104 1.41e-13 63.5
MsG0580027342.01.T02 AT5G23260 44.444 81 39 2 1 75 24 104 1.63e-13 63.2
MsG0580027342.01.T02 AT3G61120 50.000 62 31 0 1 62 8 69 2.21e-13 62.8
MsG0580027342.01.T02 AT2G22540 48.387 62 27 1 1 62 8 64 2.96e-13 62.4
MsG0580027342.01.T02 AT5G15800 50.794 63 30 1 1 62 8 70 4.89e-13 62.0
MsG0580027342.01.T02 AT5G15800 50.794 63 30 1 1 62 8 70 5.13e-13 62.0
MsG0580027342.01.T02 AT2G03710 56.000 50 22 0 1 50 8 57 7.37e-13 60.5
MsG0580027342.01.T02 AT5G65050 46.032 63 33 1 1 62 8 70 8.82e-13 60.1
MsG0580027342.01.T02 AT5G65050 46.032 63 33 1 1 62 8 70 1.59e-12 59.7
MsG0580027342.01.T02 AT3G02310 46.032 63 33 1 1 62 8 70 1.59e-12 59.7
MsG0580027342.01.T02 AT5G65050 46.032 63 33 1 1 62 8 70 1.64e-12 59.7
MsG0580027342.01.T02 AT5G65050 46.032 63 33 1 1 62 8 70 1.66e-12 59.7
MsG0580027342.01.T02 AT2G03710 56.000 50 22 0 1 50 8 57 2.26e-12 60.1
MsG0580027342.01.T02 AT2G03710 56.000 50 22 0 1 50 8 57 2.33e-12 60.1
MsG0580027342.01.T02 AT1G29962 39.062 64 39 0 1 64 14 77 3.67e-12 58.5
MsG0580027342.01.T02 AT3G02310 46.032 63 33 1 1 62 8 70 4.05e-12 59.3
MsG0580027342.01.T02 AT5G20240 43.750 64 34 1 1 62 8 71 4.33e-12 57.8
MsG0580027342.01.T02 AT5G65060 46.032 63 33 1 1 62 8 70 5.25e-12 58.2
MsG0580027342.01.T02 AT5G65060 46.032 63 33 1 1 62 8 70 5.96e-12 58.2
MsG0580027342.01.T02 AT5G65060 46.032 63 33 1 1 62 8 70 6.10e-12 58.2
MsG0580027342.01.T02 AT5G20240 43.750 64 34 1 1 62 8 71 6.57e-12 58.2
MsG0580027342.01.T02 AT1G24260 56.000 50 22 0 1 50 8 57 7.93e-12 58.5
MsG0580027342.01.T02 AT3G58780 48.077 52 27 0 1 52 23 74 8.55e-12 58.5
MsG0580027342.01.T02 AT1G24260 56.000 50 22 0 1 50 8 57 9.32e-12 58.5
MsG0580027342.01.T02 AT1G24260 56.000 50 22 0 1 50 8 57 9.74e-12 58.2
MsG0580027342.01.T02 AT3G58780 48.077 52 27 0 1 52 23 74 1.12e-11 58.2
MsG0580027342.01.T02 AT1G28460 39.062 64 39 0 1 64 14 77 1.75e-11 57.0
MsG0580027342.01.T02 AT5G65070 40.541 74 40 2 1 73 8 78 1.76e-11 56.2
MsG0580027342.01.T02 AT5G65070 40.541 74 40 2 1 73 8 78 4.62e-11 55.8
MsG0580027342.01.T02 AT5G65070 40.541 74 40 2 1 73 8 78 6.08e-11 55.8
MsG0580027342.01.T02 AT5G65070 40.541 74 40 2 1 73 8 78 7.14e-11 55.8
MsG0580027342.01.T02 AT5G49490 43.478 69 32 2 10 71 60 128 7.31e-11 56.2
MsG0580027342.01.T02 AT1G18750 36.620 71 43 1 1 69 8 78 7.53e-11 56.2
MsG0580027342.01.T02 AT1G18750 36.620 71 43 1 1 69 8 78 8.13e-11 56.2
MsG0580027342.01.T02 AT1G47760 41.176 51 30 0 11 61 18 68 8.50e-11 55.1
MsG0580027342.01.T02 AT1G18750 36.620 71 43 1 1 69 8 78 8.74e-11 56.2

Find 11 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCATGATGTCATCATTT+TGG 0.109779 5:+58707662 None:intergenic
TCTATGATCGTTTCAACATT+TGG 0.289536 5:+58707709 None:intergenic
AGCGAGCTTTGCACCCTTTG+TGG 0.386397 5:-58707790 MsG0580027342.01.T01:CDS
CGCAATATATGCACCACAAA+GGG 0.465418 5:+58707777 None:intergenic
AATGAAAATACTTTCTCAGC+AGG 0.517037 5:+58707739 None:intergenic
CTTTCTCGAAGCGCCGTAGT+GGG 0.553366 5:-58707828 MsG0580027342.01.T01:CDS
ACTTTCTCGAAGCGCCGTAG+TGG 0.571963 5:-58707829 MsG0580027342.01.T01:CDS
TGTGCTTTAGCCATGTCAAG+CGG 0.619256 5:-58707919 None:intergenic
TCAAGCGGAAAGAAAACTCA+AGG 0.621333 5:-58707904 MsG0580027342.01.T01:CDS
CTTTCTTGAAGAGCCCACTA+CGG 0.627906 5:+58707815 None:intergenic
ACGCAATATATGCACCACAA+AGG 0.628806 5:+58707776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATGAAAATACTTTCTCAGC+AGG + Chr5:58707831-58707850 None:intergenic 30.0%
TCTATGATCGTTTCAACATT+TGG + Chr5:58707861-58707880 None:intergenic 30.0%
! ATTGCATGATGTCATCATTT+TGG + Chr5:58707908-58707927 None:intergenic 30.0%
! TTGCATGATGTCATCATTTT+GGG + Chr5:58707907-58707926 None:intergenic 30.0%
!!! GCTGAGAAAGTATTTTCATT+TGG - Chr5:58707831-58707850 MsG0580027342.01.T01:CDS 30.0%
ACGCAATATATGCACCACAA+AGG + Chr5:58707794-58707813 None:intergenic 40.0%
CGCAATATATGCACCACAAA+GGG + Chr5:58707793-58707812 None:intergenic 40.0%
TCAAGCGGAAAGAAAACTCA+AGG - Chr5:58707663-58707682 MsG0580027342.01.T01:CDS 40.0%
! ATGATGTCATCATTTTGGGC+TGG + Chr5:58707903-58707922 None:intergenic 40.0%
CTTTCTTGAAGAGCCCACTA+CGG + Chr5:58707755-58707774 None:intergenic 45.0%
AGCGAGCTTTGCACCCTTTG+TGG - Chr5:58707777-58707796 MsG0580027342.01.T01:CDS 55.0%
!! ACTTTCTCGAAGCGCCGTAG+TGG - Chr5:58707738-58707757 MsG0580027342.01.T01:CDS 55.0%
!! CTTTCTCGAAGCGCCGTAGT+GGG - Chr5:58707739-58707758 MsG0580027342.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 58707660 58707929 58707660 ID=MsG0580027342.01;Name=MsG0580027342.01
Chr5 mRNA 58707660 58707929 58707660 ID=MsG0580027342.01.T01;Parent=MsG0580027342.01;Name=MsG0580027342.01.T01;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|89
Chr5 exon 58707660 58707929 58707660 ID=MsG0580027342.01.T01:exon:13202;Parent=MsG0580027342.01.T01
Chr5 CDS 58707660 58707929 58707660 ID=MsG0580027342.01.T01:cds;Parent=MsG0580027342.01.T01
Chr5 mRNA 58707660 58707887 58707660 ID=MsG0580027342.01.T02;Parent=MsG0580027342.01;Name=MsG0580027342.01.T02;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|0|1|0|75
Chr5 exon 58707660 58707887 58707660 ID=MsG0580027342.01.T02:exon:13203;Parent=MsG0580027342.01.T02
Chr5 CDS 58707660 58707887 58707660 ID=MsG0580027342.01.T02:cds;Parent=MsG0580027342.01.T02
Gene Sequence

>MsG0580027342.01.T01

ATGTCAAGCGGAAAGAAAACTCAAGGTCGTAAAAAGATTGAGATGAAAAAGATGAGCAATGAAAGTAACTTGCAAGTGACTTTCTCGAAGCGCCGTAGTGGGCTCTTCAAGAAAGCTAGCGAGCTTTGCACCCTTTGTGGTGCATATATTGCGTTAATCATATTCTCGCCTGCTGAGAAAGTATTTTCATTTGGTCATCCAAATGTTGAAACGATCATAGATCGTTATCTCTCTCAAGTTCCAGCCCAAAATGATGACATCATGCAATAA

>MsG0580027342.01.T02

ATGAAAAAGATGAGCAATGAAAGTAACTTGCAAGTGACTTTCTCGAAGCGCCGTAGTGGGCTCTTCAAGAAAGCTAGCGAGCTTTGCACCCTTTGTGGTGCATATATTGCGTTAATCATATTCTCGCCTGCTGAGAAAGTATTTTCATTTGGTCATCCAAATGTTGAAACGATCATAGATCGTTATCTCTCTCAAGTTCCAGCCCAAAATGATGACATCATGCAATAA

Protein sequence

>MsG0580027342.01.T01

MSSGKKTQGRKKIEMKKMSNESNLQVTFSKRRSGLFKKASELCTLCGAYIALIIFSPAEKVFSFGHPNVETIIDRYLSQVPAQNDDIMQ*

>MsG0580027342.01.T02

MKKMSNESNLQVTFSKRRSGLFKKASELCTLCGAYIALIIFSPAEKVFSFGHPNVETIIDRYLSQVPAQNDDIMQ*