AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027485.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027485.01.T01 MTR_5g061890 91.029 379 26 2 1 376 1 374 0.0 702
MsG0580027485.01.T01 MTR_5g060780 89.710 379 31 2 1 376 1 374 0.0 689
MsG0580027485.01.T01 MTR_5g460920 89.710 379 31 2 1 376 1 374 0.0 687
MsG0580027485.01.T01 MTR_5g060630 54.916 417 135 8 1 367 1 414 1.90e-133 392
MsG0580027485.01.T01 MTR_5g460740 54.916 417 135 8 1 367 1 414 1.90e-133 392
MsG0580027485.01.T01 MTR_5g061220 53.924 395 141 9 1 364 8 392 4.15e-129 381
MsG0580027485.01.T01 MTR_1g058210 53.543 381 141 10 1 364 1 362 1.22e-119 354
MsG0580027485.01.T01 MTR_2g024430 52.381 357 136 16 1 341 9 347 1.32e-103 315
MsG0580027485.01.T01 MTR_5g040880 49.558 339 146 9 8 335 7 331 1.95e-95 295
MsG0580027485.01.T01 MTR_5g040740 51.506 332 126 11 1 315 1 314 8.02e-90 276
MsG0580027485.01.T01 MTR_2g006270 45.052 384 180 14 6 374 7 374 1.04e-87 273
MsG0580027485.01.T01 MTR_5g082140 45.954 346 158 10 6 336 6 337 2.53e-87 271
MsG0580027485.01.T01 MTR_5g060770 53.094 307 88 7 61 365 7 259 1.60e-84 261
MsG0580027485.01.T01 MTR_2g006380 42.520 381 187 14 6 374 14 374 1.25e-83 262
MsG0580027485.01.T01 MTR_5g460910 47.003 317 89 10 52 365 14 254 7.50e-65 210
MsG0580027485.01.T01 MTR_5g062970 57.143 196 68 7 12 195 38 229 1.47e-62 201
MsG0580027485.01.T01 MTR_3g073420 33.571 420 201 11 9 378 16 407 5.09e-61 206
MsG0580027485.01.T01 MTR_1g064430 36.253 411 191 14 9 360 24 422 1.19e-57 199
MsG0580027485.01.T01 MTR_1g064430 36.253 411 191 14 9 360 37 435 1.43e-57 199
MsG0580027485.01.T01 MTR_1g094960 35.468 406 190 16 9 359 16 404 2.61e-57 197
MsG0580027485.01.T01 MTR_2g094570 34.828 379 186 12 9 336 8 376 5.07e-55 192
MsG0580027485.01.T01 MTR_2g094570 34.828 379 186 12 9 336 8 376 6.53e-55 191
MsG0580027485.01.T01 MTR_2g094570 34.828 379 186 12 9 336 64 432 1.07e-54 192
MsG0580027485.01.T01 MTR_4g058930 35.641 390 183 13 9 340 9 388 2.53e-53 187
MsG0580027485.01.T01 MTR_4g058930 36.154 390 181 13 9 340 9 388 3.56e-53 187
MsG0580027485.01.T01 MTR_7g101280 30.024 423 209 13 9 361 28 433 3.35e-48 173
MsG0580027485.01.T01 MTR_0041s0110 30.024 423 209 13 9 361 28 433 3.35e-48 173
MsG0580027485.01.T01 MTR_7g101275 32.381 315 175 11 22 314 2 300 5.10e-40 146
MsG0580027485.01.T01 MTR_5g062960 63.393 112 40 1 227 338 1 111 1.09e-39 140
MsG0580027485.01.T01 MTR_2g014770 29.381 388 230 15 4 364 43 413 1.19e-38 146
MsG0580027485.01.T01 MTR_2g014770 29.381 388 230 15 4 364 43 413 1.66e-38 146
MsG0580027485.01.T01 MTR_4g021580 28.691 359 236 8 12 358 19 369 2.42e-37 142
MsG0580027485.01.T01 MTR_4g021580 28.691 359 236 8 12 358 19 369 3.98e-37 142
MsG0580027485.01.T01 MTR_2g005240 28.238 386 232 10 12 371 23 389 6.36e-37 142
MsG0580027485.01.T01 MTR_0041s0120 31.173 324 179 11 22 314 2 312 7.31e-36 135
MsG0580027485.01.T01 MTR_7g062540 28.333 360 239 8 12 359 14 366 7.82e-36 139
MsG0580027485.01.T01 MTR_8g100050 28.448 348 210 9 12 335 59 391 3.02e-35 137
MsG0580027485.01.T01 MTR_8g100050 28.448 348 210 9 12 335 59 391 8.42e-35 136
MsG0580027485.01.T01 MTR_2g018690 29.651 344 219 10 9 335 20 357 1.94e-34 135
MsG0580027485.01.T01 MTR_4g088210 29.620 368 212 15 12 351 38 386 4.54e-34 134
MsG0580027485.01.T01 MTR_4g088210 29.620 368 212 15 12 351 38 386 5.96e-34 133
MsG0580027485.01.T01 MTR_8g027440 29.067 375 244 11 12 373 29 394 7.58e-34 133
MsG0580027485.01.T01 MTR_4g124900 27.763 389 236 12 9 361 20 399 8.44e-32 127
MsG0580027485.01.T01 MTR_4g124900 27.806 392 232 12 9 361 20 399 9.97e-32 127
MsG0580027485.01.T01 MTR_5g076270 29.326 341 218 10 12 335 24 358 3.06e-31 125
MsG0580027485.01.T01 MTR_2g043250 29.070 344 219 11 9 335 20 355 3.88e-31 125
MsG0580027485.01.T01 MTR_8g079492 28.249 354 210 12 9 335 20 356 4.27e-31 125
MsG0580027485.01.T01 MTR_8g079492 28.249 354 210 12 9 335 20 356 4.36e-31 125
MsG0580027485.01.T01 MTR_1g024025 29.912 341 215 11 12 335 43 376 1.15e-30 124
MsG0580027485.01.T01 MTR_3g064050 29.032 341 219 10 12 335 26 360 4.04e-30 122
MsG0580027485.01.T01 MTR_2g093740 28.715 397 237 16 12 376 56 438 3.71e-28 116
MsG0580027485.01.T01 MTR_2g093740 28.715 397 237 16 12 376 56 438 4.08e-28 116
MsG0580027485.01.T01 MTR_2g093740 28.715 397 237 16 12 376 56 438 5.63e-28 116
MsG0580027485.01.T01 MTR_4g060460 28.354 395 240 15 12 376 54 435 2.24e-27 114
MsG0580027485.01.T01 MTR_8g101360 28.409 352 213 11 9 335 28 365 8.83e-27 112
MsG0580027485.01.T01 MTR_4g060460 28.205 390 237 15 12 371 54 430 3.26e-26 110
MsG0580027485.01.T01 MTR_2g093740 27.805 410 237 17 12 376 56 451 3.90e-25 107
MsG0580027485.01.T01 MTR_2g093740 27.805 410 237 17 12 376 56 451 4.58e-25 107
MsG0580027485.01.T01 MTR_2g093740 27.805 410 237 17 12 376 56 451 6.14e-25 107
MsG0580027485.01.T01 MTR_8g079492 27.178 287 175 9 70 335 1 274 2.64e-20 93.2
MsG0580027485.01.T01 MTR_8g446900 27.181 298 173 12 61 335 12 288 2.07e-19 89.4
MsG0580027485.01.T01 MTR_2g093740 28.114 281 176 11 112 376 38 308 2.93e-15 77.8
MsG0580027485.01.T01 MTR_2g093740 28.114 281 176 11 112 376 38 308 3.52e-15 77.4
MsG0580027485.01.T01 MTR_5g046120 36.752 117 68 3 50 160 12 128 1.46e-14 70.5
MsG0580027485.01.T01 MTR_2g093740 26.871 294 176 12 112 376 38 321 2.47e-12 68.6
MsG0580027485.01.T01 MTR_2g093740 26.871 294 176 12 112 376 38 321 2.81e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027485.01.T01 AT1G77850 35.027 374 188 13 8 336 15 378 8.35e-62 209
MsG0580027485.01.T01 AT1G77850 35.027 374 188 13 8 336 15 378 9.91e-62 208
MsG0580027485.01.T01 AT4G30080 35.550 391 191 14 9 347 16 397 1.12e-52 186
MsG0580027485.01.T01 AT2G28350 33.756 394 186 14 9 340 7 387 3.50e-52 184
MsG0580027485.01.T01 AT2G28350 33.756 394 186 14 9 340 7 387 4.74e-52 184
MsG0580027485.01.T01 AT2G46530 30.579 363 225 9 12 358 42 393 3.25e-42 156
MsG0580027485.01.T01 AT2G46530 30.579 363 225 9 12 358 21 372 3.84e-42 155
MsG0580027485.01.T01 AT3G61830 30.145 345 219 8 12 342 25 361 1.56e-41 154
MsG0580027485.01.T01 AT1G34390 30.769 377 214 14 12 364 27 380 7.56e-39 146
MsG0580027485.01.T01 AT1G59750 31.414 382 231 13 12 374 22 391 2.09e-38 146
MsG0580027485.01.T01 AT1G59750 31.398 379 229 13 12 371 22 388 5.72e-38 145
MsG0580027485.01.T01 AT1G59750 31.398 379 229 13 12 371 22 388 5.72e-38 145
MsG0580027485.01.T01 AT1G35520 31.525 387 200 17 12 364 27 382 6.20e-38 144
MsG0580027485.01.T01 AT1G59750 31.694 366 219 13 12 358 22 375 7.36e-38 144
MsG0580027485.01.T01 AT1G34410 30.184 381 213 15 12 364 27 382 1.25e-36 140
MsG0580027485.01.T01 AT1G34310 30.729 384 213 15 9 364 24 382 5.72e-36 138
MsG0580027485.01.T01 AT5G62000 28.818 347 210 10 12 335 61 393 4.78e-35 137
MsG0580027485.01.T01 AT1G35540 30.829 386 204 19 12 364 27 382 5.13e-35 136
MsG0580027485.01.T01 AT5G62000 28.818 347 210 10 12 335 61 393 5.28e-35 137
MsG0580027485.01.T01 AT5G62000 28.818 347 210 10 12 335 61 393 5.28e-35 137
MsG0580027485.01.T01 AT5G62000 28.818 347 210 10 12 335 61 393 5.28e-35 137
MsG0580027485.01.T01 AT5G62000 28.818 347 210 10 12 335 61 393 5.28e-35 137
MsG0580027485.01.T01 AT4G23980 28.530 347 223 9 12 340 12 351 1.91e-34 134
MsG0580027485.01.T01 AT4G23980 28.530 347 223 9 12 340 12 351 2.21e-34 134
MsG0580027485.01.T01 AT1G35240 31.063 367 189 17 12 343 27 364 5.64e-34 133
MsG0580027485.01.T01 AT5G37020 30.409 342 213 12 12 335 22 356 3.45e-33 131
MsG0580027485.01.T01 AT1G30330 29.412 340 219 9 12 335 23 357 3.98e-33 131
MsG0580027485.01.T01 AT1G30330 29.412 340 219 9 12 335 25 359 4.14e-33 131
MsG0580027485.01.T01 AT1G30330 29.412 340 219 9 12 335 25 359 4.14e-33 131
MsG0580027485.01.T01 AT5G37020 30.409 342 213 12 12 335 22 356 4.75e-33 131
MsG0580027485.01.T01 AT1G34170 30.000 360 211 14 12 349 25 365 2.70e-32 127
MsG0580027485.01.T01 AT2G33860 28.792 389 230 13 12 378 54 417 6.19e-32 127
MsG0580027485.01.T01 AT1G19850 30.347 346 218 10 7 335 38 377 4.34e-31 125
MsG0580027485.01.T01 AT1G19850 30.347 346 218 10 7 335 49 388 4.88e-31 125
MsG0580027485.01.T01 AT1G19220 28.161 348 217 10 9 335 20 355 1.92e-30 123
MsG0580027485.01.T01 AT3G61830 30.272 294 188 6 59 342 24 310 4.48e-30 121
MsG0580027485.01.T01 AT5G20730 28.736 348 216 11 9 335 21 357 1.84e-29 120
MsG0580027485.01.T01 AT5G20730 28.736 348 216 11 9 335 21 357 2.05e-29 120
MsG0580027485.01.T01 AT5G20730 28.242 347 218 10 9 335 21 356 4.92e-29 119
MsG0580027485.01.T01 AT5G60450 28.354 395 239 16 12 376 65 445 8.83e-28 115
MsG0580027485.01.T01 AT5G60450 28.354 395 239 16 12 376 41 421 9.15e-28 115
MsG0580027485.01.T01 AT1G43950 32.353 204 118 6 12 203 27 222 3.19e-26 105
MsG0580027485.01.T01 AT1G34170 28.011 357 196 13 12 349 25 339 6.15e-26 108
MsG0580027485.01.T01 AT1G34170 28.011 357 196 13 12 349 25 339 8.06e-26 109
MsG0580027485.01.T01 AT2G46530 30.233 258 162 7 111 358 36 285 1.23e-23 102
MsG0580027485.01.T01 AT2G33860 28.315 279 168 10 112 378 2 260 1.36e-19 90.5
MsG0580027485.01.T01 AT5G37020 29.545 264 165 10 85 335 6 261 1.98e-17 84.7

Find 98 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGCATATCTGCCATATTT+TGG 0.143600 5:-61934286 None:intergenic
AACTGATGAGATTAAGGTTT+TGG 0.252605 5:+61934914 MsG0580027485.01.T01:CDS
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5:-61934327 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5:+61935162 MsG0580027485.01.T01:CDS
TGTCTCCAACATCGTCTTCT+TGG 0.287447 5:-61934554 None:intergenic
GATGACAATTTCTCAGCTTA+AGG 0.289847 5:+61935115 MsG0580027485.01.T01:CDS
GAATTTAACTGATGAGATTA+AGG 0.298587 5:+61934908 MsG0580027485.01.T01:CDS
CGACCCTGCCAAGGTTTGTT+GGG 0.299743 5:-61935795 None:intergenic
TGCCAAGGATTGACTAGGTT+TGG 0.313165 5:-61935677 None:intergenic
GATCTTTCCCAAGCTTGATT+TGG 0.314401 5:+61934656 MsG0580027485.01.T01:CDS
ACCTCAAGCATGCGCCAATT+AGG 0.318991 5:-61935176 None:intergenic
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5:+61935044 MsG0580027485.01.T01:CDS
CTACTTCGCACGAGGTCATC+TGG 0.355762 5:+61934350 MsG0580027485.01.T01:CDS
TCGACCCTGCCAAGGTTTGT+TGG 0.360193 5:-61935796 None:intergenic
GAACCATTCTTGATCGTTAC+CGG 0.364840 5:+61934406 MsG0580027485.01.T01:CDS
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5:-61935175 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5:-61934312 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5:-61935042 None:intergenic
TCCAACATCGTCTTCTTGGC+CGG 0.392440 5:-61934550 None:intergenic
ATGACAATTTCTCAGCTTAA+GGG 0.392754 5:+61935116 MsG0580027485.01.T01:CDS
CTCGTGTGGAACCCAAAATA+TGG 0.402726 5:+61934274 MsG0580027485.01.T01:CDS
GCCGGGAACTACAGGAGGAG+GGG 0.417463 5:-61934532 None:intergenic
TACGGGAGTCAGAAGCAGTT+TGG 0.422184 5:-61934496 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.424216 5:-61934871 None:intergenic
AATTCCGACAGATATCTTTC+CGG 0.425831 5:-61934838 None:intergenic
TGGCCGGGAACTACAGGAGG+AGG 0.428083 5:-61934534 None:intergenic
CGACCTTAAAAGCCGAGAAT+GGG 0.441124 5:+61934704 MsG0580027485.01.T01:CDS
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5:-61934307 None:intergenic
CCAAGGTTTGGCTATGTGTC+AGG 0.447799 5:-61935830 None:intergenic
GACTCGTCCAAATCAAGCTT+GGG 0.456946 5:-61934663 None:intergenic
GTGTATTATCCGACAGCTAT+TGG 0.461034 5:+61934978 MsG0580027485.01.T01:CDS
TCTTCTTGGCCGGGAACTAC+AGG 0.462528 5:-61934540 None:intergenic
CCGGGAACTACAGGAGGAGG+GGG 0.475602 5:-61934531 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5:+61935032 MsG0580027485.01.T01:CDS
GATGAAAACAACAGAATCGT+TGG 0.490573 5:-61934805 None:intergenic
AGACTCGTCCAAATCAAGCT+TGG 0.490804 5:-61934664 None:intergenic
GAAGATTCATATTTCTTCAA+AGG 0.492904 5:-61935083 None:intergenic
ATGGTTCTTGTGGCAGCAGT+AGG 0.493323 5:-61934390 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.493859 5:+61935635 MsG0580027485.01.T01:intron
CCGACCTTAAAAGCCGAGAA+TGG 0.494002 5:+61934703 MsG0580027485.01.T01:CDS
GACCCTGCCAAGGTTTGTTG+GGG 0.496074 5:-61935794 None:intergenic
GGCGGAGTTGACGGTGGAGG+AGG 0.498559 5:-61935735 None:intergenic
TGCGGCGGAGTTGACGGTGG+AGG 0.500479 5:-61935738 None:intergenic
ATCCAAACCTAGTCAATCCT+TGG 0.508187 5:+61935675 MsG0580027485.01.T01:CDS
CAACCTGCCAAGGATTGACT+AGG 0.513736 5:-61935682 None:intergenic
TCATCCTCTGCCACCTCGTG+TGG 0.517268 5:+61934260 MsG0580027485.01.T01:CDS
CCAACATCGTCTTCTTGGCC+GGG 0.517848 5:-61934549 None:intergenic
AATCTCAGTAGATTACATAC+TGG 0.519388 5:+61934747 MsG0580027485.01.T01:CDS
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5:+61935175 MsG0580027485.01.T01:CDS
GGTTCCACACGAGGTGGCAG+AGG 0.527487 5:-61934264 None:intergenic
GAGACGATGCATGGAATGGA+TGG 0.527501 5:-61934429 None:intergenic
GGAAGAGACGATGCATGGAA+TGG 0.530637 5:-61934433 None:intergenic
TGGTTCTTGTGGCAGCAGTA+GGG 0.533159 5:-61934389 None:intergenic
TGACTAGGTTTGGATTCTGC+GGG 0.536979 5:-61935667 None:intergenic
GAGGAGGAAGTCGAAGGTGC+AGG 0.538875 5:-61935719 None:intergenic
AGATATCTGTCGGAATTCGC+AGG 0.543135 5:+61934844 MsG0580027485.01.T01:CDS
ACTACAGATGAAGCGGATGA+AGG 0.543135 5:+61934879 MsG0580027485.01.T01:CDS
CCCTGCCAAGGTTTGTTGGG+GGG 0.543970 5:-61935792 None:intergenic
TTTGGATGCAGTGGAATTGG+CGG 0.544683 5:+61934932 MsG0580027485.01.T01:CDS
TTGTAAACTTCAACCTGCCA+AGG 0.544687 5:-61935692 None:intergenic
AAACCTAGTCAATCCTTGGC+AGG 0.546100 5:+61935679 MsG0580027485.01.T01:CDS
GATGAACTGTCAGCAAGTCT+AGG 0.552115 5:-61935770 None:intergenic
GCAAAGACCTCGTCGGTGTG+TGG 0.555611 5:-61934474 None:intergenic
ACGATGCCATGAAGATTGGT+TGG 0.558168 5:+61935036 MsG0580027485.01.T01:CDS
ACCCTGCCAAGGTTTGTTGG+GGG 0.559662 5:-61935793 None:intergenic
TCAGTAGATTACATACTGGC+TGG 0.560711 5:+61934751 MsG0580027485.01.T01:CDS
CGATGCATGGAATGGATGGC+CGG 0.561229 5:-61934425 None:intergenic
GGTTCTTGTGGCAGCAGTAG+GGG 0.568653 5:-61934388 None:intergenic
ATTATCCGACAGCTATTGGT+TGG 0.574808 5:+61934982 MsG0580027485.01.T01:CDS
GGCCGGGAACTACAGGAGGA+GGG 0.579671 5:-61934533 None:intergenic
TTGACTAGGTTTGGATTCTG+CGG 0.581555 5:-61935668 None:intergenic
TGGCCGGTAACGATCAAGAA+TGG 0.583189 5:-61934409 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.584809 5:+61934872 MsG0580027485.01.T01:CDS
AAAATATGGCAGATATGCGC+CGG 0.589425 5:+61934288 MsG0580027485.01.T01:CDS
CCCGGCCAAGAAGACGATGT+TGG 0.598087 5:+61934549 MsG0580027485.01.T01:CDS
TGACAATTTCTCAGCTTAAG+GGG 0.599563 5:+61935117 MsG0580027485.01.T01:CDS
TAATCTACTGAGATTTGCAT+AGG 0.604127 5:-61934739 None:intergenic
TCAACGGAAGAGACGATGCA+TGG 0.604313 5:-61934438 None:intergenic
CAAACCTTGGCAGGGTCGAG+TGG 0.615213 5:+61935800 MsG0580027485.01.T01:CDS
GACCTTAAAAGCCGAGAATG+GGG 0.616896 5:+61934705 MsG0580027485.01.T01:CDS
ACAGATGAAGCGGATGAAGG+AGG 0.630115 5:+61934882 MsG0580027485.01.T01:CDS
AATCTTTGGAATCTTGACGT+CGG 0.630358 5:-61934313 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.631537 5:-61934400 None:intergenic
TCACGCCAACCAATAGCTGT+CGG 0.635734 5:-61934987 None:intergenic
AGTACCACTCGACCCTGCCA+AGG 0.642496 5:-61935804 None:intergenic
TCTGCCGGAAAGATATCTGT+CGG 0.642648 5:+61934834 MsG0580027485.01.T01:CDS
TTGCGTGAGAGTCTTGACAT+AGG 0.647637 5:-61934586 None:intergenic
TCTTGCGGCGGAGTTGACGG+TGG 0.648657 5:-61935741 None:intergenic
TGGATCAACAAAGAGATCAA+CGG 0.649094 5:-61934454 None:intergenic
ACCTTAAAAGCCGAGAATGG+GGG 0.652954 5:+61934706 MsG0580027485.01.T01:CDS
TCTTGGCCGGGAACTACAGG+AGG 0.657068 5:-61934537 None:intergenic
AGGTGGCAGAGGATGAGACA+TGG 0.663852 5:-61934253 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5:-61934311 None:intergenic
CAGTTTGGCAAAGACCTCGT+CGG 0.665831 5:-61934481 None:intergenic
AAAGTTTACTACTTCGCACG+AGG 0.667496 5:+61934342 MsG0580027485.01.T01:CDS
ATCAAGTTAGAGCATTCGAT+AGG 0.670484 5:-61934636 None:intergenic
TGTTGATCCACACACCGACG+AGG 0.698520 5:+61934467 MsG0580027485.01.T01:CDS
ACGGTGGAGGAGGAAGTCGA+AGG 0.704506 5:-61935725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTATCTTTTTATCAT+AGG + Chr5:61935622-61935641 MsG0580027485.01.T01:intron 15.0%
!! TCAATCAAATAATCTTATCA+TGG - Chr5:61935255-61935274 None:intergenic 20.0%
! AAAAGTTTCTACTTAGCATT+AGG - Chr5:61935533-61935552 None:intergenic 25.0%
! GAAGATTCATATTTCTTCAA+AGG - Chr5:61935086-61935105 None:intergenic 25.0%
! GAATTTAACTGATGAGATTA+AGG + Chr5:61934908-61934927 MsG0580027485.01.T01:CDS 25.0%
! GTACATATGCTTTCTTAATT+TGG + Chr5:61935197-61935216 MsG0580027485.01.T01:intron 25.0%
!! CTTTTTATCATAGGTTAACT+GGG + Chr5:61935631-61935650 MsG0580027485.01.T01:intron 25.0%
!! TCTTTTTATCATAGGTTAAC+TGG + Chr5:61935630-61935649 MsG0580027485.01.T01:intron 25.0%
!!! CTATTTTTAGTGAATATCAC+TGG - Chr5:61935465-61935484 None:intergenic 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG - Chr5:61934330-61934349 None:intergenic 25.0%
AATCTCAGTAGATTACATAC+TGG + Chr5:61934747-61934766 MsG0580027485.01.T01:CDS 30.0%
ATGACAATTTCTCAGCTTAA+GGG + Chr5:61935116-61935135 MsG0580027485.01.T01:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG + Chr5:61935162-61935181 MsG0580027485.01.T01:CDS 30.0%
TAATCTACTGAGATTTGCAT+AGG - Chr5:61934742-61934761 None:intergenic 30.0%
! AACTGATGAGATTAAGGTTT+TGG + Chr5:61934914-61934933 MsG0580027485.01.T01:CDS 30.0%
!! AGGAGTATTTACAATGAGAA+AGG - Chr5:61935513-61935532 None:intergenic 30.0%
AATTCCGACAGATATCTTTC+CGG - Chr5:61934841-61934860 None:intergenic 35.0%
AGATTTCCAACCAATCTTCA+TGG - Chr5:61935045-61935064 None:intergenic 35.0%
CTATCAAGTGCATGAACAAT+GGG - Chr5:61935384-61935403 None:intergenic 35.0%
GATGAAAACAACAGAATCGT+TGG - Chr5:61934808-61934827 None:intergenic 35.0%
GATGACAATTTCTCAGCTTA+AGG + Chr5:61935115-61935134 MsG0580027485.01.T01:CDS 35.0%
TACAATGAGAAAGGCTTAGA+AGG - Chr5:61935504-61935523 None:intergenic 35.0%
TCTATCAAGTGCATGAACAA+TGG - Chr5:61935385-61935404 None:intergenic 35.0%
TGACAATTTCTCAGCTTAAG+GGG + Chr5:61935117-61935136 MsG0580027485.01.T01:CDS 35.0%
TGGATCAACAAAGAGATCAA+CGG - Chr5:61934457-61934476 None:intergenic 35.0%
TTATCATAGGTTAACTGGGA+TGG + Chr5:61935635-61935654 MsG0580027485.01.T01:intron 35.0%
! AAGTCTAGGAGTTTTTCTTG+CGG - Chr5:61935759-61935778 None:intergenic 35.0%
! ATCAAGTTAGAGCATTCGAT+AGG - Chr5:61934639-61934658 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG + Chr5:61935044-61935063 MsG0580027485.01.T01:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+CGG + Chr5:61934819-61934838 MsG0580027485.01.T01:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG - Chr5:61934316-61934335 None:intergenic 35.0%
AAAATATGGCAGATATGCGC+CGG + Chr5:61934288-61934307 MsG0580027485.01.T01:CDS 40.0%
AAAGTTTACTACTTCGCACG+AGG + Chr5:61934342-61934361 MsG0580027485.01.T01:CDS 40.0%
ACGATCAAGAATGGTTCTTG+TGG - Chr5:61934403-61934422 None:intergenic 40.0%
ATCCAAACCTAGTCAATCCT+TGG + Chr5:61935675-61935694 MsG0580027485.01.T01:CDS 40.0%
ATTATCCGACAGCTATTGGT+TGG + Chr5:61934982-61935001 MsG0580027485.01.T01:CDS 40.0%
CAAGTTCACTACAGATGAAG+CGG + Chr5:61934872-61934891 MsG0580027485.01.T01:CDS 40.0%
GAACCATTCTTGATCGTTAC+CGG + Chr5:61934406-61934425 MsG0580027485.01.T01:CDS 40.0%
GATCTTTCCCAAGCTTGATT+TGG + Chr5:61934656-61934675 MsG0580027485.01.T01:CDS 40.0%
GTGTATTATCCGACAGCTAT+TGG + Chr5:61934978-61934997 MsG0580027485.01.T01:CDS 40.0%
TATATGTGTACCCCCATTCT+CGG - Chr5:61934719-61934738 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG + Chr5:61934751-61934770 MsG0580027485.01.T01:CDS 40.0%
TTGACTAGGTTTGGATTCTG+CGG - Chr5:61935671-61935690 None:intergenic 40.0%
TTGTAAACTTCAACCTGCCA+AGG - Chr5:61935695-61935714 None:intergenic 40.0%
! GCGCATATCTGCCATATTTT+GGG - Chr5:61934288-61934307 None:intergenic 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG - Chr5:61934315-61934334 None:intergenic 40.0%
!! TATTGGTTGGCGTGATTTTG+TGG + Chr5:61934995-61935014 MsG0580027485.01.T01:CDS 40.0%
!!! GATTAAGGTTTTGGATGCAG+TGG + Chr5:61934923-61934942 MsG0580027485.01.T01:CDS 40.0%
AAACCTAGTCAATCCTTGGC+AGG + Chr5:61935679-61935698 MsG0580027485.01.T01:CDS 45.0%
ACCTTAAAAGCCGAGAATGG+GGG + Chr5:61934706-61934725 MsG0580027485.01.T01:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG + Chr5:61935036-61935055 MsG0580027485.01.T01:CDS 45.0%
ACTACAGATGAAGCGGATGA+AGG + Chr5:61934879-61934898 MsG0580027485.01.T01:CDS 45.0%
AGACTCGTCCAAATCAAGCT+TGG - Chr5:61934667-61934686 None:intergenic 45.0%
AGATATCTGTCGGAATTCGC+AGG + Chr5:61934844-61934863 MsG0580027485.01.T01:CDS 45.0%
CGACCTTAAAAGCCGAGAAT+GGG + Chr5:61934704-61934723 MsG0580027485.01.T01:CDS 45.0%
CGCTTCATCTGTAGTGAACT+TGG - Chr5:61934874-61934893 None:intergenic 45.0%
CTCGTGTGGAACCCAAAATA+TGG + Chr5:61934274-61934293 MsG0580027485.01.T01:CDS 45.0%
GACCTTAAAAGCCGAGAATG+GGG + Chr5:61934705-61934724 MsG0580027485.01.T01:CDS 45.0%
GACTCGTCCAAATCAAGCTT+GGG - Chr5:61934666-61934685 None:intergenic 45.0%
GATGAACTGTCAGCAAGTCT+AGG - Chr5:61935773-61935792 None:intergenic 45.0%
GTTGACGATGCCATGAAGAT+TGG + Chr5:61935032-61935051 MsG0580027485.01.T01:CDS 45.0%
TCTGCCGGAAAGATATCTGT+CGG + Chr5:61934834-61934853 MsG0580027485.01.T01:CDS 45.0%
TGACTAGGTTTGGATTCTGC+GGG - Chr5:61935670-61935689 None:intergenic 45.0%
TGCCAAGGATTGACTAGGTT+TGG - Chr5:61935680-61935699 None:intergenic 45.0%
TGTCTCCAACATCGTCTTCT+TGG - Chr5:61934557-61934576 None:intergenic 45.0%
TTGCGTGAGAGTCTTGACAT+AGG - Chr5:61934589-61934608 None:intergenic 45.0%
TTTGGATGCAGTGGAATTGG+CGG + Chr5:61934932-61934951 MsG0580027485.01.T01:CDS 45.0%
! GGCGCATATCTGCCATATTT+TGG - Chr5:61934289-61934308 None:intergenic 45.0%
! TCTAGGAGTTTTTCTTGCGG+CGG - Chr5:61935756-61935775 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG - Chr5:61934314-61934333 None:intergenic 45.0%
!!! CATATTTTGGGTTCCACACG+AGG - Chr5:61934276-61934295 None:intergenic 45.0%
!!! CCATTCTCGGCTTTTAAGGT+CGG - Chr5:61934706-61934725 None:intergenic 45.0%
!!! GGTTTTGGATGCAGTGGAAT+TGG + Chr5:61934929-61934948 MsG0580027485.01.T01:CDS 45.0%
ACAGATGAAGCGGATGAAGG+AGG + Chr5:61934882-61934901 MsG0580027485.01.T01:CDS 50.0%
ACCTCAAGCATGCGCCAATT+AGG - Chr5:61935179-61935198 None:intergenic 50.0%
AGGGGGTTCTTGGTCAGTTA+CGG - Chr5:61934517-61934536 None:intergenic 50.0%
ATGGTTCTTGTGGCAGCAGT+AGG - Chr5:61934393-61934412 None:intergenic 50.0%
CAACCTGCCAAGGATTGACT+AGG - Chr5:61935685-61935704 None:intergenic 50.0%
CAGTTTGGCAAAGACCTCGT+CGG - Chr5:61934484-61934503 None:intergenic 50.0%
CCGACCTTAAAAGCCGAGAA+TGG + Chr5:61934703-61934722 MsG0580027485.01.T01:CDS 50.0%
CCTCAAGCATGCGCCAATTA+GGG - Chr5:61935178-61935197 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG - Chr5:61934432-61934451 None:intergenic 50.0%
GGAAGAGACGATGCATGGAA+TGG - Chr5:61934436-61934455 None:intergenic 50.0%
TACGGGAGTCAGAAGCAGTT+TGG - Chr5:61934499-61934518 None:intergenic 50.0%
TCAACGGAAGAGACGATGCA+TGG - Chr5:61934441-61934460 None:intergenic 50.0%
TCACGCCAACCAATAGCTGT+CGG - Chr5:61934990-61935009 None:intergenic 50.0%
TCCAACATCGTCTTCTTGGC+CGG - Chr5:61934553-61934572 None:intergenic 50.0%
TGGCCGGTAACGATCAAGAA+TGG - Chr5:61934412-61934431 None:intergenic 50.0%
TGGTTCTTGTGGCAGCAGTA+GGG - Chr5:61934392-61934411 None:intergenic 50.0%
TTCATCCCCCCAACAAACCT+TGG + Chr5:61935787-61935806 MsG0580027485.01.T01:CDS 50.0%
! ACCCCCATTCTCGGCTTTTA+AGG - Chr5:61934710-61934729 None:intergenic 50.0%
! TGGTTGGCGTGATTTTGTGG+TGG + Chr5:61934998-61935017 MsG0580027485.01.T01:CDS 50.0%
! TTTTCTTGCGGCGGAGTTGA+CGG - Chr5:61935747-61935766 None:intergenic 50.0%
!!! ATTTTGGGTTCCACACGAGG+TGG - Chr5:61934273-61934292 None:intergenic 50.0%
CCAACATCGTCTTCTTGGCC+GGG - Chr5:61934552-61934571 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTG+AGG + Chr5:61935175-61935194 MsG0580027485.01.T01:CDS 55.0%
CGATGCATGGAATGGATGGC+CGG - Chr5:61934428-61934447 None:intergenic 55.0%
CTACTTCGCACGAGGTCATC+TGG + Chr5:61934350-61934369 MsG0580027485.01.T01:CDS 55.0%
GGGGGTTCTTGGTCAGTTAC+GGG - Chr5:61934516-61934535 None:intergenic 55.0%
GGTTCTTGTGGCAGCAGTAG+GGG - Chr5:61934391-61934410 None:intergenic 55.0%
TCGACCCTGCCAAGGTTTGT+TGG - Chr5:61935799-61935818 None:intergenic 55.0%
TCTTCTTGGCCGGGAACTAC+AGG - Chr5:61934543-61934562 None:intergenic 55.0%
TGTTGATCCACACACCGACG+AGG + Chr5:61934467-61934486 MsG0580027485.01.T01:CDS 55.0%
! CGACCCTGCCAAGGTTTGTT+GGG - Chr5:61935798-61935817 None:intergenic 55.0%
!! ACCCTGCCAAGGTTTGTTGG+GGG - Chr5:61935796-61935815 None:intergenic 55.0%
!! GACCCTGCCAAGGTTTGTTG+GGG - Chr5:61935797-61935816 None:intergenic 55.0%
ACGGTGGAGGAGGAAGTCGA+AGG - Chr5:61935728-61935747 None:intergenic 60.0%
AGTACCACTCGACCCTGCCA+AGG - Chr5:61935807-61935826 None:intergenic 60.0%
CAAACCTTGGCAGGGTCGAG+TGG + Chr5:61935800-61935819 MsG0580027485.01.T01:CDS 60.0%
CCCCCCAACAAACCTTGGCA+GGG + Chr5:61935792-61935811 MsG0580027485.01.T01:CDS 60.0%
CCCGGCCAAGAAGACGATGT+TGG + Chr5:61934549-61934568 MsG0580027485.01.T01:CDS 60.0%
CTACAGGAGGAGGGGGTTCT+TGG - Chr5:61934527-61934546 None:intergenic 60.0%
GCAAAGACCTCGTCGGTGTG+TGG - Chr5:61934477-61934496 None:intergenic 60.0%
TCATCCTCTGCCACCTCGTG+TGG + Chr5:61934260-61934279 MsG0580027485.01.T01:CDS 60.0%
TCCCCCCAACAAACCTTGGC+AGG + Chr5:61935791-61935810 MsG0580027485.01.T01:CDS 60.0%
TCTTGGCCGGGAACTACAGG+AGG - Chr5:61934540-61934559 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG - Chr5:61934310-61934329 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG - Chr5:61935722-61935741 None:intergenic 60.0%
!! CCCTGCCAAGGTTTGTTGGG+GGG - Chr5:61935795-61935814 None:intergenic 60.0%
CCCCCTCCTCCTGTAGTTCC+CGG + Chr5:61934531-61934550 MsG0580027485.01.T01:CDS 65.0%
CCGGGAACTACAGGAGGAGG+GGG - Chr5:61934534-61934553 None:intergenic 65.0%
GCCGGGAACTACAGGAGGAG+GGG - Chr5:61934535-61934554 None:intergenic 65.0%
GGCCGGGAACTACAGGAGGA+GGG - Chr5:61934536-61934555 None:intergenic 65.0%
GGTTCCACACGAGGTGGCAG+AGG - Chr5:61934267-61934286 None:intergenic 65.0%
TCTTGCGGCGGAGTTGACGG+TGG - Chr5:61935744-61935763 None:intergenic 65.0%
TGGCCGGGAACTACAGGAGG+AGG - Chr5:61934537-61934556 None:intergenic 65.0%
GGCGGAGTTGACGGTGGAGG+AGG - Chr5:61935738-61935757 None:intergenic 70.0%
TGCGGCGGAGTTGACGGTGG+AGG - Chr5:61935741-61935760 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 61934255 61935841 61934255 ID=MsG0580027485.01;Name=MsG0580027485.01
Chr5 mRNA 61934255 61935841 61934255 ID=MsG0580027485.01.T01;Parent=MsG0580027485.01;Name=MsG0580027485.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|2|0|379
Chr5 exon 61934255 61935196 61934255 ID=MsG0580027485.01.T01:exon:13609;Parent=MsG0580027485.01.T01
Chr5 exon 61935644 61935841 61935644 ID=MsG0580027485.01.T01:exon:13610;Parent=MsG0580027485.01.T01
Chr5 CDS 61934255 61935196 61934255 ID=MsG0580027485.01.T01:cds;Parent=MsG0580027485.01.T01
Chr5 CDS 61935644 61935841 61935644 ID=MsG0580027485.01.T01:cds;Parent=MsG0580027485.01.T01
Gene Sequence

>MsG0580027485.01.T01

ATGTCTCATCCTCTGCCACCTCGTGTGGAACCCAAAATATGGCAGATATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCGCACGAGGTCATCTGGAACATGCATGTTCTTCCCCTACTGCTGCCACAAGAACCATTCTTGATCGTTACCGGCCATCCATTCCATGCATCGTCTCTTCCGTTGATCTCTTTGTTGATCCACACACCGACGAGGTCTTTGCCAAACTGCTTCTGACTCCCGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGGCCAAGAAGACGATGTTGGAGACAATCTTGTTTCCTATGTCAAGACTCTCACGCAATCTGATTGTGCAAGAGTATTATGTGTACCTATCGAATGCTCTAACTTGATCTTTCCCAAGCTTGATTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAAGCCGAGAATGGGGGTACACATATACCTATGCAAATCTCAGTAGATTACATACTGGCTGGTTAAATTTTGTACGAGAGAAGAAACTCGTCGCCAACGATTCTGTTGTTTTCATCAAAAACTCTGCCGGAAAGATATCTGTCGGAATTCGCAGGAACACCAAGTTCACTACAGATGAAGCGGATGAAGGAGGCGAGAATTTAACTGATGAGATTAAGGTTTTGGATGCAGTGGAATTGGCGGAGAAGAACACAGCATTTGATGTTGTGTATTATCCGACAGCTATTGGTTGGCGTGATTTTGTGGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAACTTCCTTTGAAGAAATATGAATCTTCAAGTTCAAAGATGACAATTTCTCAGCTTAAGGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTTAACTGGGATGGACTTGATTTCCCGCAGAATCCAAACCTAGTCAATCCTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTCGACTTCCTCCTCCACCGTCAACTCCGCCGCAAGAAAAACTCCTAGACTTGCTGACAGTTCATCCCCCCAACAAACCTTGGCAGGGTCGAGTGGTACTTTGCCTGACACATAG
Protein sequence

>MsG0580027485.01.T01

MSHPLPPRVEPKIWQICAGPDVKIPKIHSKVYYFARGHLEHACSSPTAATRTILDRYRPSIPCIVSSVDLFVDPHTDEVFAKLLLTPVTDQEPPPPVVPGQEDDVGDNLVSYVKTLTQSDCARVLCVPIECSNLIFPKLDLDESQSITVTDLKSREWGYTYTYANLSRLHTGWLNFVREKKLVANDSVVFIKNSAGKISVGIRRNTKFTTDEADEGGENLTDEIKVLDAVELAEKNTAFDVVYYPTAIGWRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKYESSSSKMTISQLKGTISFVFNDSSNVPNWRMLEVNWDGLDFPQNPNLVNPWQVEVYNIPAPSTSSSTVNSAARKTPRLADSSSPQQTLAGSSGTLPDT*