AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027543.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027543.01.T01 MTR_5g060780 68.011 372 98 8 1 357 9 374 3.47e-165 476
MsG0580027543.01.T01 MTR_5g061890 68.407 364 95 7 1 349 9 367 8.11e-165 474
MsG0580027543.01.T01 MTR_5g460920 67.742 372 99 8 1 357 9 374 2.18e-164 474
MsG0580027543.01.T01 MTR_5g061220 48.764 445 159 11 1 389 16 447 2.21e-128 386
MsG0580027543.01.T01 MTR_5g060630 53.431 408 126 8 1 345 9 415 1.48e-127 384
MsG0580027543.01.T01 MTR_5g460740 53.431 408 126 8 1 345 9 415 1.48e-127 384
MsG0580027543.01.T01 MTR_1g058210 53.056 360 144 9 1 341 9 362 1.29e-116 353
MsG0580027543.01.T01 MTR_2g024430 49.189 370 159 11 1 351 17 376 1.61e-103 322
MsG0580027543.01.T01 MTR_5g040880 50.904 332 139 9 1 316 8 331 1.05e-96 305
MsG0580027543.01.T01 MTR_5g082140 40.389 411 207 13 1 385 9 407 6.17e-86 275
MsG0580027543.01.T01 MTR_2g006270 44.663 356 171 9 4 341 13 360 4.25e-85 272
MsG0580027543.01.T01 MTR_2g006380 43.840 349 167 10 4 333 20 358 7.96e-82 263
MsG0580027543.01.T01 MTR_5g040740 49.367 316 132 9 1 297 9 315 1.70e-81 261
MsG0580027543.01.T01 MTR_5g060770 50.485 309 94 8 53 354 7 263 8.63e-74 239
MsG0580027543.01.T01 MTR_3g073420 33.843 523 246 17 1 455 16 506 7.99e-66 225
MsG0580027543.01.T01 MTR_5g062970 53.846 208 74 7 3 193 37 239 1.14e-62 206
MsG0580027543.01.T01 MTR_1g064430 33.259 451 214 13 1 373 37 478 2.00e-57 204
MsG0580027543.01.T01 MTR_1g064430 33.259 451 214 13 1 373 24 465 2.37e-57 204
MsG0580027543.01.T01 MTR_2g094570 34.896 384 178 13 1 322 8 381 7.41e-55 197
MsG0580027543.01.T01 MTR_2g094570 34.375 384 180 12 1 322 8 381 1.12e-54 196
MsG0580027543.01.T01 MTR_2g094570 34.896 384 178 13 1 322 64 437 1.72e-54 197
MsG0580027543.01.T01 MTR_5g460910 43.034 323 96 10 43 354 13 258 2.54e-54 187
MsG0580027543.01.T01 MTR_1g094960 33.010 412 191 14 1 347 16 407 6.55e-53 191
MsG0580027543.01.T01 MTR_4g058930 34.805 385 173 12 1 317 9 383 1.52e-50 185
MsG0580027543.01.T01 MTR_4g058930 34.805 385 173 12 1 317 9 383 1.98e-50 185
MsG0580027543.01.T01 MTR_7g101280 29.130 460 224 14 1 373 28 472 1.18e-47 176
MsG0580027543.01.T01 MTR_0041s0110 29.130 460 224 14 1 373 28 472 1.18e-47 176
MsG0580027543.01.T01 MTR_2g005240 32.029 409 208 15 4 377 23 396 1.44e-42 162
MsG0580027543.01.T01 MTR_2g018690 33.973 365 179 18 1 320 20 367 1.48e-39 155
MsG0580027543.01.T01 MTR_7g101275 31.250 320 173 10 14 301 2 306 1.35e-38 146
MsG0580027543.01.T01 MTR_8g079492 32.687 361 188 15 1 320 20 366 2.00e-38 151
MsG0580027543.01.T01 MTR_8g079492 32.687 361 188 15 1 320 20 366 2.49e-38 151
MsG0580027543.01.T01 MTR_8g100050 32.344 337 200 11 4 316 59 391 2.55e-38 151
MsG0580027543.01.T01 MTR_8g100050 32.344 337 200 11 4 316 59 391 2.89e-38 150
MsG0580027543.01.T01 MTR_5g076270 32.782 363 186 16 1 320 21 368 2.13e-37 148
MsG0580027543.01.T01 MTR_1g024025 31.564 358 204 12 1 324 40 390 1.24e-36 146
MsG0580027543.01.T01 MTR_2g043250 31.686 344 199 12 1 316 20 355 4.02e-36 144
MsG0580027543.01.T01 MTR_2g014770 30.056 356 203 13 4 325 53 396 6.60e-36 142
MsG0580027543.01.T01 MTR_2g093740 32.153 367 207 13 4 335 56 415 7.18e-36 143
MsG0580027543.01.T01 MTR_4g021580 30.000 370 216 14 2 343 17 371 7.93e-36 142
MsG0580027543.01.T01 MTR_2g093740 32.153 367 207 13 4 335 56 415 8.16e-36 142
MsG0580027543.01.T01 MTR_2g093740 32.153 367 207 13 4 335 56 415 8.83e-36 143
MsG0580027543.01.T01 MTR_4g021580 30.000 370 216 14 2 343 17 371 9.40e-36 142
MsG0580027543.01.T01 MTR_2g014770 30.056 356 203 13 4 325 53 396 1.07e-35 142
MsG0580027543.01.T01 MTR_3g064050 31.492 362 192 15 1 320 23 370 1.10e-35 143
MsG0580027543.01.T01 MTR_5g062960 44.262 183 85 5 211 390 1 169 1.11e-35 132
MsG0580027543.01.T01 MTR_4g124900 32.174 345 196 14 1 316 20 355 8.06e-35 140
MsG0580027543.01.T01 MTR_4g124900 32.174 345 196 14 1 316 20 355 9.20e-35 140
MsG0580027543.01.T01 MTR_7g062540 28.455 369 212 13 4 336 14 366 1.66e-34 139
MsG0580027543.01.T01 MTR_4g088210 31.034 377 208 15 4 342 38 400 2.23e-34 138
MsG0580027543.01.T01 MTR_8g027440 31.034 348 215 12 4 331 29 371 3.17e-34 138
MsG0580027543.01.T01 MTR_4g088210 30.585 376 211 14 4 342 38 400 3.21e-34 138
MsG0580027543.01.T01 MTR_2g093740 31.053 380 207 13 4 335 56 428 5.47e-34 137
MsG0580027543.01.T01 MTR_2g093740 31.053 380 207 13 4 335 56 428 6.71e-34 137
MsG0580027543.01.T01 MTR_2g093740 31.053 380 207 13 4 335 56 428 7.35e-34 137
MsG0580027543.01.T01 MTR_0041s0120 30.030 333 172 12 14 301 2 318 6.96e-32 128
MsG0580027543.01.T01 MTR_8g101360 30.878 353 192 11 1 316 28 365 1.03e-29 125
MsG0580027543.01.T01 MTR_4g060460 29.950 404 231 14 4 373 54 439 9.72e-29 122
MsG0580027543.01.T01 MTR_4g060460 30.108 372 214 12 4 343 54 411 1.02e-28 121
MsG0580027543.01.T01 MTR_8g079492 31.081 296 155 13 62 320 1 284 2.74e-24 108
MsG0580027543.01.T01 MTR_2g093740 30.597 268 159 9 93 335 20 285 5.15e-20 94.4
MsG0580027543.01.T01 MTR_2g093740 30.597 268 159 9 93 335 20 285 6.73e-20 94.0
MsG0580027543.01.T01 MTR_8g446900 27.645 293 167 13 53 316 12 288 2.53e-18 87.4
MsG0580027543.01.T01 MTR_2g093740 29.181 281 159 9 93 335 20 298 2.90e-18 89.0
MsG0580027543.01.T01 MTR_2g093740 29.181 281 159 9 93 335 20 298 3.68e-18 88.6
MsG0580027543.01.T01 MTR_5g046120 34.711 121 69 4 42 153 12 131 7.40e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027543.01.T01 AT1G77850 34.163 442 211 15 1 373 16 446 1.15e-66 227
MsG0580027543.01.T01 AT1G77850 34.163 442 211 15 1 373 16 446 1.58e-66 227
MsG0580027543.01.T01 AT4G30080 32.755 461 222 14 1 386 16 463 1.02e-54 196
MsG0580027543.01.T01 AT2G28350 33.165 395 167 15 1 317 7 382 9.18e-47 174
MsG0580027543.01.T01 AT2G28350 33.165 395 167 15 1 317 7 382 9.23e-47 174
MsG0580027543.01.T01 AT3G61830 33.679 386 207 16 3 359 24 389 5.71e-45 168
MsG0580027543.01.T01 AT2G46530 32.973 370 200 15 3 343 20 370 1.48e-42 161
MsG0580027543.01.T01 AT2G46530 32.973 370 200 15 3 343 41 391 1.70e-42 161
MsG0580027543.01.T01 AT5G37020 32.240 366 184 17 1 320 19 366 9.24e-41 158
MsG0580027543.01.T01 AT5G37020 32.240 366 184 17 1 320 19 366 1.63e-40 157
MsG0580027543.01.T01 AT1G30330 32.222 360 192 14 1 320 22 369 7.56e-39 152
MsG0580027543.01.T01 AT1G30330 32.222 360 192 14 1 320 22 369 7.56e-39 152
MsG0580027543.01.T01 AT1G30330 32.222 360 192 14 1 320 20 367 7.61e-39 152
MsG0580027543.01.T01 AT1G59750 32.938 337 197 11 4 316 22 353 1.41e-38 150
MsG0580027543.01.T01 AT1G59750 32.938 337 197 11 4 316 22 353 1.44e-38 150
MsG0580027543.01.T01 AT1G59750 32.938 337 197 11 4 316 22 353 1.71e-38 150
MsG0580027543.01.T01 AT1G59750 32.938 337 197 11 4 316 22 353 1.71e-38 150
MsG0580027543.01.T01 AT2G33860 32.796 372 196 16 4 338 54 408 1.28e-37 147
MsG0580027543.01.T01 AT1G19850 31.768 362 197 13 1 323 40 390 2.72e-37 148
MsG0580027543.01.T01 AT1G19850 31.768 362 197 13 1 323 51 401 3.20e-37 148
MsG0580027543.01.T01 AT5G62000 30.612 343 198 10 4 316 61 393 1.29e-35 143
MsG0580027543.01.T01 AT5G62000 30.612 343 198 10 4 316 61 393 1.31e-35 143
MsG0580027543.01.T01 AT5G62000 30.612 343 198 10 4 316 61 393 1.31e-35 143
MsG0580027543.01.T01 AT5G62000 30.612 343 198 10 4 316 61 393 1.31e-35 143
MsG0580027543.01.T01 AT5G62000 30.612 343 198 10 4 316 61 393 1.31e-35 143
MsG0580027543.01.T01 AT1G34390 31.778 343 191 12 1 316 24 350 2.99e-34 137
MsG0580027543.01.T01 AT1G19220 31.609 348 194 13 1 316 20 355 5.18e-34 138
MsG0580027543.01.T01 AT1G35520 31.884 345 190 13 1 316 24 352 6.90e-34 136
MsG0580027543.01.T01 AT5G20730 31.304 345 200 13 1 316 21 357 1.42e-33 137
MsG0580027543.01.T01 AT5G20730 31.304 345 200 13 1 316 21 357 1.42e-33 137
MsG0580027543.01.T01 AT4G23980 28.877 374 213 13 4 345 12 364 1.52e-33 135
MsG0580027543.01.T01 AT1G34410 31.304 345 192 13 1 316 24 352 1.64e-33 135
MsG0580027543.01.T01 AT4G23980 28.877 374 213 13 4 345 12 364 1.76e-33 135
MsG0580027543.01.T01 AT1G35240 32.174 345 189 14 1 316 24 352 1.96e-33 135
MsG0580027543.01.T01 AT5G20730 31.594 345 198 14 1 316 21 356 2.00e-33 136
MsG0580027543.01.T01 AT1G35540 30.548 383 222 14 1 355 24 390 2.86e-33 134
MsG0580027543.01.T01 AT5G60450 30.601 366 214 12 3 335 64 422 3.88e-33 135
MsG0580027543.01.T01 AT5G60450 30.601 366 214 12 3 335 40 398 4.49e-33 135
MsG0580027543.01.T01 AT1G34310 32.164 342 187 13 4 316 27 352 1.70e-32 132
MsG0580027543.01.T01 AT3G61830 32.659 346 188 15 39 359 13 338 3.06e-31 128
MsG0580027543.01.T01 AT1G34170 28.851 409 232 17 1 369 22 411 2.75e-29 122
MsG0580027543.01.T01 AT1G34170 28.070 399 222 15 1 369 22 385 1.06e-23 105
MsG0580027543.01.T01 AT1G34170 28.070 399 222 15 1 369 22 385 1.43e-23 105
MsG0580027543.01.T01 AT5G37020 31.071 280 143 14 77 320 6 271 1.40e-22 102
MsG0580027543.01.T01 AT2G46530 32.319 263 141 12 103 343 36 283 2.37e-22 101
MsG0580027543.01.T01 AT2G33860 32.061 262 139 12 104 338 2 251 3.35e-22 100
MsG0580027543.01.T01 AT1G43950 33.333 207 114 7 1 191 24 222 6.40e-22 95.1

Find 159 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTTATCATAGGTTAAA+TGG 0.116737 5:-63042483 MsG0580027543.01.T01:intron
TGTTTCCATCGTCGTCTTCT+TGG 0.207983 5:+63043529 None:intergenic
ATAGTTAAATTTGAAGATTC+AGG 0.238302 5:+63043012 None:intergenic
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5:+63043756 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5:-63042954 MsG0580027543.01.T01:CDS
GGTGGTTTCTGCGGGATATT+AGG 0.279342 5:+63042454 None:intergenic
TCTTGAGACTTCTGCAACTC+TGG 0.293011 5:+63043420 None:intergenic
ATGGTGATTGTGGCAGCATT+AGG 0.295879 5:+63043693 None:intergenic
CGCAGAGTCGACTGATACTT+TGG 0.299747 5:-63042092 MsG0580027543.01.T01:CDS
TCACTTGGTGCTTGGCTCAT+TGG 0.308376 5:+63041667 None:intergenic
ACCTTAAGCATGCGCCAATT+AGG 0.312806 5:+63042940 None:intergenic
CTTGGTCTGTTACGGGAGTT+AGG 0.314595 5:+63043577 None:intergenic
CTACTTCCCACAAGGTCATT+TGG 0.318755 5:-63043733 MsG0580027543.01.T01:CDS
CTCGACTCTGCGCCCAAGTC+AGG 0.332469 5:+63042034 None:intergenic
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5:-63043063 MsG0580027543.01.T01:CDS
CAGTTCTAAAGGTGCGGACT+TGG 0.342634 5:-63041771 MsG0580027543.01.T01:CDS
CAGTTCTAAAGGTGCGGACT+TGG 0.342634 5:-63041909 MsG0580027543.01.T01:CDS
CAGTTCTAAAGGTGCGGACT+TGG 0.342634 5:-63041978 MsG0580027543.01.T01:CDS
CTCTTTATCATAGGTTAAAT+GGG 0.347475 5:-63042482 MsG0580027543.01.T01:intron
TCTTCAAATTTAACTATAAG+AGG 0.347983 5:-63043006 MsG0580027543.01.T01:CDS
CCAAGCTTTAGCCTTGTATC+AGG 0.353230 5:+63042127 None:intergenic
TGGTGATTGTGGCAGCATTA+GGG 0.354813 5:+63043694 None:intergenic
AGCGCATTTCTGCCATAGTT+TGG 0.354882 5:+63043797 None:intergenic
GATGAAAACAACGGAATCTT+CGG 0.357948 5:+63043290 None:intergenic
TTCTCACCTCTTTCAGCTGC+AGG 0.358959 5:+63043216 None:intergenic
CCCTAATTGGCGCATGCTTA+AGG 0.360706 5:-63042941 MsG0580027543.01.T01:intron
GAGGGCAAAGCTTCGTTTGA+TGG 0.363032 5:-63043037 MsG0580027543.01.T01:CDS
AGTGGTACTTTGCTTGATAC+AGG 0.364586 5:-63042208 MsG0580027543.01.T01:CDS
CCACAGGAGGAGGAGGTTCT+TGG 0.364923 5:+63043559 None:intergenic
GCCACAATCACCATTCTTGA+TGG 0.366631 5:-63043684 MsG0580027543.01.T01:CDS
CCTTAAGCATGCGCCAATTA+GGG 0.368474 5:+63042941 None:intergenic
ATTAACTGAGAGTGCGGTCT+TGG 0.368633 5:-63043193 MsG0580027543.01.T01:CDS
GTCTCTGTCACTGATCTTCC+AGG 0.369479 5:-63043396 MsG0580027543.01.T01:CDS
AGGAGGTTCTTGGTCTGTTA+CGG 0.370719 5:+63043569 None:intergenic
CAGTTCTAAAGGTGCGGACT+TGG 0.371782 5:-63041840 MsG0580027543.01.T01:CDS
GAAGAACTCTGAGATAGTTT+AGG 0.377530 5:+63042355 None:intergenic
TTTATCCCACAGCTGGGAAT+TGG 0.381505 5:-63043125 MsG0580027543.01.T01:CDS
AGATTTCCAACCAATCTTCA+TGG 0.383662 5:+63043065 None:intergenic
AGTGATACTTTGGCTGATAC+AGG 0.384060 5:-63041875 MsG0580027543.01.T01:CDS
AGTGATACTTTGGCTGATAC+AGG 0.384060 5:-63041944 MsG0580027543.01.T01:CDS
AGTGATACTTTGGCTGATAC+AGG 0.384060 5:-63042013 MsG0580027543.01.T01:CDS
ATGAAAACAACGGAATCTTC+GGG 0.384466 5:+63043291 None:intergenic
AGTGATACTTTGGCTGATAC+AGG 0.388252 5:-63041806 MsG0580027543.01.T01:CDS
ACACAAACTGATACTAAATC+TGG 0.395790 5:-63043480 MsG0580027543.01.T01:CDS
GGAGGTTCTTGGTCTGTTAC+GGG 0.397553 5:+63043570 None:intergenic
GGTCTTGGGTGCAGTGGATT+TGG 0.411620 5:-63043178 MsG0580027543.01.T01:CDS
GTGTTGAGTTGTTGCCTCAT+AGG 0.419590 5:-63041691 MsG0580027543.01.T01:CDS
CTCCTATGGACTCCAAACTA+TGG 0.424679 5:-63043809 None:intergenic
TGACCAGGGGTGGTTTCTGC+GGG 0.425488 5:+63042446 None:intergenic
CGCAGAGGCGAGTGATACTT+TGG 0.437352 5:-63041885 MsG0580027543.01.T01:CDS
TACGGGAGTTAGGAGCAGTT+TGG 0.451525 5:+63043587 None:intergenic
GGTAATGGTGTCAGTGTACT+TGG 0.452357 5:+63042304 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.453371 5:+63043771 None:intergenic
CGCAGAGTCGAGTGATACTT+TGG 0.455775 5:-63041747 MsG0580027543.01.T01:CDS
CGCAGAGTCGAGTGATACTT+TGG 0.455775 5:-63041954 MsG0580027543.01.T01:CDS
CGCAGAGTCGAGTGATACTT+TGG 0.455775 5:-63042023 MsG0580027543.01.T01:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5:-63041851 MsG0580027543.01.T01:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5:-63041920 MsG0580027543.01.T01:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5:-63041989 MsG0580027543.01.T01:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.461797 5:-63041713 MsG0580027543.01.T01:CDS
ATAAACAGTGGAGGCGGTAA+TGG 0.462686 5:+63042289 None:intergenic
CTAAAGCTTGGCAGTTCTAA+AGG 0.463200 5:-63042115 MsG0580027543.01.T01:CDS
CACAGAGTCGAGTGATACTT+TGG 0.463899 5:-63041816 MsG0580027543.01.T01:CDS
CATCTGGCATGCAAGGAGTC+AGG 0.465776 5:-63042261 MsG0580027543.01.T01:CDS
TTAACTGAGAGTGCGGTCTT+GGG 0.478339 5:-63043192 MsG0580027543.01.T01:CDS
ACAGTGGAGGAGGAAGTTGA+TGG 0.480871 5:+63042400 None:intergenic
AACTTCCCTGCAGCTGAAAG+AGG 0.482726 5:-63043222 MsG0580027543.01.T01:CDS
ATAAAGCTTGGCAGTTCTAA+AGG 0.485741 5:-63041782 MsG0580027543.01.T01:CDS
GTATAGGTGTAGACTTTGCC+TGG 0.494583 5:+63043378 None:intergenic
GTAGATGATGCCATGAAGAT+TGG 0.497274 5:-63043075 MsG0580027543.01.T01:CDS
CATGGCATCATCTACAACCT+TGG 0.499201 5:+63043083 None:intergenic
AGTTCTAAAGGTGCGGACTT+GGG 0.500873 5:-63041770 MsG0580027543.01.T01:CDS
AGTTCTAAAGGTGCGGACTT+GGG 0.500873 5:-63041908 MsG0580027543.01.T01:CDS
AGTTCTAAAGGTGCGGACTT+GGG 0.500873 5:-63041977 MsG0580027543.01.T01:CDS
TTGACCAGGGGTGGTTTCTG+CGG 0.502646 5:+63042445 None:intergenic
TCGACTCTGCGCCCAAGTCA+GGG 0.505174 5:+63042035 None:intergenic
AACCACCCCTGGTCAATCCA+TGG 0.506086 5:-63042438 MsG0580027543.01.T01:CDS
AAGATACCTCACTTGGTGCT+TGG 0.507669 5:+63041659 None:intergenic
CTTGATACAGGGCTAAAGCT+TGG 0.508063 5:-63042196 MsG0580027543.01.T01:CDS
TCTTCTTGGCCGTGAACCAC+AGG 0.514079 5:+63043543 None:intergenic
CCTCCTCCTCCTGTGGTTCA+CGG 0.519022 5:-63043552 MsG0580027543.01.T01:CDS
GAAGGTGCTGACTTGTTCGC+AGG 0.520378 5:-63042235 MsG0580027543.01.T01:CDS
CAACCTCCCATGGATTGACC+AGG 0.523330 5:+63042431 None:intergenic
GGAGATGTTGACAGTGGAGG+AGG 0.528074 5:+63042390 None:intergenic
GCTGATACAGTGCTAAAGCT+TGG 0.530738 5:-63041725 MsG0580027543.01.T01:CDS
GCTGATACAGGGATAAAGCT+TGG 0.532500 5:-63041794 MsG0580027543.01.T01:CDS
GCTGATACAGGGCTAAAGCT+TGG 0.532624 5:-63041863 MsG0580027543.01.T01:CDS
GCTGATACAGGGCTAAAGCT+TGG 0.532624 5:-63041932 MsG0580027543.01.T01:CDS
GCTGATACAGGGCTAAAGCT+TGG 0.532624 5:-63042001 MsG0580027543.01.T01:CDS
GCTGATACAGAGCTAAAGCT+TGG 0.537434 5:-63042070 MsG0580027543.01.T01:CDS
TGGCCGTGAACCACAGGAGG+AGG 0.540091 5:+63043549 None:intergenic
CCATGCATCGTCTCTACTGT+TGG 0.543464 5:-63043645 MsG0580027543.01.T01:CDS
AAATATATGTCGGAATTCGC+AGG 0.543716 5:-63043251 MsG0580027543.01.T01:CDS
CCTGATACAAGGCTAAAGCT+TGG 0.543990 5:-63042127 MsG0580027543.01.T01:CDS
GAGACGATGCATGGAATGGA+TGG 0.546365 5:+63043654 None:intergenic
AGTTCTAAAGGTGCGGACTT+GGG 0.546645 5:-63041839 MsG0580027543.01.T01:CDS
TTGGTTGGAAATCTGGAATG+AGG 0.547760 5:-63043056 MsG0580027543.01.T01:CDS
CTTGGCTCATTGGACCTATG+AGG 0.550878 5:+63041677 None:intergenic
TCCCATGGATTGACCAGGGG+TGG 0.554649 5:+63042436 None:intergenic
ATGATGCCATGAAGATTGGT+TGG 0.558711 5:-63043071 MsG0580027543.01.T01:CDS
CCGTGAACCACAGGAGGAGG+AGG 0.561880 5:+63043552 None:intergenic
GGGTAAACTTCAACCTCCCA+TGG 0.562049 5:+63042421 None:intergenic
GTGGTACTTTGCCTGATACA+AGG 0.562508 5:-63042138 MsG0580027543.01.T01:CDS
GAGTGCGGTCTTGGGTGCAG+TGG 0.564030 5:-63043184 MsG0580027543.01.T01:CDS
ATGCAAGGAGTCAGGCAAGA+AGG 0.567091 5:-63042253 MsG0580027543.01.T01:CDS
AAACTATGGCAGAAATGCGC+TGG 0.567239 5:-63043795 MsG0580027543.01.T01:CDS
AGTAGAGACGATGCATGGAA+TGG 0.572932 5:+63043650 None:intergenic
GACTTGTTCGCAGGGTCGAG+TGG 0.575371 5:-63042226 MsG0580027543.01.T01:CDS
AAACAGAGTAGACTAACTAA+AGG 0.575699 5:-63043348 MsG0580027543.01.T01:CDS
TGTTGTGGAGATGTTGACAG+TGG 0.578376 5:+63042384 None:intergenic
TCTCACCTCTTTCAGCTGCA+GGG 0.578496 5:+63043217 None:intergenic
CTGACTCCTTGCATGCCAGA+TGG 0.584361 5:+63042262 None:intergenic
CATGTTCCAAATGACCTTGT+GGG 0.588081 5:+63043727 None:intergenic
GGTGATTGTGGCAGCATTAG+GGG 0.588227 5:+63043695 None:intergenic
TGGTTGGAAATCTGGAATGA+GGG 0.589287 5:-63043055 MsG0580027543.01.T01:CDS
TGGACGGTAACCATCAAGAA+TGG 0.589526 5:+63043674 None:intergenic
ACCATCAAGAATGGTGATTG+TGG 0.590838 5:+63043683 None:intergenic
GTGGTACTTTGCTTGATACA+GGG 0.591580 5:-63042207 MsG0580027543.01.T01:CDS
ATTACACCAATTCCCAGCTG+TGG 0.592913 5:+63043119 None:intergenic
CCAAGAACCTCCTCCTCCTG+TGG 0.595254 5:-63043559 MsG0580027543.01.T01:CDS
CAGTGGAGGAGGAAGTTGAT+GGG 0.598002 5:+63042401 None:intergenic
GTGATACTTTGGCTGATACA+GGG 0.598282 5:-63041943 MsG0580027543.01.T01:CDS
GTGATACTTTGGCTGATACA+GGG 0.598282 5:-63042012 MsG0580027543.01.T01:CDS
TTGTGTGAGAGTCTTGACAT+AGG 0.600208 5:+63043497 None:intergenic
TGCCATAGTTTGGAGTCCAT+AGG 0.605278 5:+63043807 None:intergenic
ATCAAGTCTGTGCATTGACG+AGG 0.606145 5:+63043447 None:intergenic
GCCAATGAAGATACCTCACT+TGG 0.608165 5:+63041652 None:intergenic
GTGATACTTTGGCTGATACA+GGG 0.608249 5:-63041874 MsG0580027543.01.T01:CDS
GCAAAGACCTCGTCGGTGTG+AGG 0.608714 5:+63043609 None:intergenic
GTGATACTTTGGCTGATACA+GGG 0.609258 5:-63041805 MsG0580027543.01.T01:CDS
TTACACCAATTCCCAGCTGT+GGG 0.610036 5:+63043120 None:intergenic
AGGTGCGGACTTGGGCGCAG+AGG 0.611587 5:-63041900 MsG0580027543.01.T01:CDS
ACCACCCCTGGTCAATCCAT+GGG 0.614274 5:-63042437 MsG0580027543.01.T01:CDS
CGATGCATGGAATGGATGGA+CGG 0.620709 5:+63043658 None:intergenic
TGTGGAGATGTTGACAGTGG+AGG 0.625945 5:+63042387 None:intergenic
AGAGTAGACTAACTAAAGGA+TGG 0.627717 5:-63043344 MsG0580027543.01.T01:CDS
AACCTCCCATGGATTGACCA+GGG 0.633534 5:+63042432 None:intergenic
GCATGTTCCAAATGACCTTG+TGG 0.633704 5:+63043726 None:intergenic
AATCTTTGGAATCTTGACGT+CGG 0.641188 5:+63043770 None:intergenic
AAGGTGCTGACTTGTTCGCA+GGG 0.642119 5:-63042234 MsG0580027543.01.T01:CDS
CCAACAGTAGAGACGATGCA+TGG 0.649094 5:+63043645 None:intergenic
TCTTGGCCGTGAACCACAGG+AGG 0.650102 5:+63043546 None:intergenic
ACCTCCCATGGATTGACCAG+GGG 0.653907 5:+63042433 None:intergenic
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5:-63041777 MsG0580027543.01.T01:CDS
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5:-63041846 MsG0580027543.01.T01:CDS
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5:-63041915 MsG0580027543.01.T01:CDS
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5:-63041984 MsG0580027543.01.T01:CDS
AATGAGCCAAGCACCAAGTG+AGG 0.655123 5:-63041665 None:intergenic
TGTTGATCCTCACACCGACG+AGG 0.655379 5:-63043616 MsG0580027543.01.T01:CDS
TATCCCGCAGAAACCACCCC+TGG 0.657644 5:-63042449 MsG0580027543.01.T01:CDS
GGATTTGGCTAACAAGAACA+TGG 0.659311 5:-63043163 MsG0580027543.01.T01:CDS
ACCCCTGGTCAATCCATGGG+AGG 0.661573 5:-63042434 MsG0580027543.01.T01:CDS
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5:+63043602 None:intergenic
GACTTGTTTGCAGAGTCGAG+TGG 0.669905 5:-63042157 MsG0580027543.01.T01:CDS
TCTGCCGGGAAAATATATGT+CGG 0.676335 5:-63043261 MsG0580027543.01.T01:CDS
AAAGTTTACTACTTCCCACA+AGG 0.678948 5:-63043741 MsG0580027543.01.T01:CDS
TGAGAAATTAACTGAGAGTG+CGG 0.683957 5:-63043199 MsG0580027543.01.T01:CDS
TGAAAACAACGGAATCTTCG+GGG 0.698750 5:+63043292 None:intergenic
CACGGCCAAGAAGACGACGA+TGG 0.706758 5:-63043534 MsG0580027543.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGATAAAGAGATAAAAAA+TGG + Chr5:63042981-63043000 None:intergenic 15.0%
!!! ATTTTTTATCTCTTTATCAT+AGG - Chr5:63042980-63042999 MsG0580027543.01.T01:CDS 15.0%
!!! TAAATCAATCTTGTTTATTA+TGG + Chr5:63042618-63042637 None:intergenic 15.0%
!!! TTAAAGAATTTTGAATTTGT+CGG + Chr5:63043145-63043164 None:intergenic 15.0%
!! ATAGTTAAATTTGAAGATTC+AGG + Chr5:63042462-63042481 None:intergenic 20.0%
!! TCTTCAAATTTAACTATAAG+AGG - Chr5:63042465-63042484 MsG0580027543.01.T01:CDS 20.0%
!!! ATAAAATTTTGAGACTTAGT+AGG - Chr5:63042679-63042698 MsG0580027543.01.T01:intron 20.0%
!!! TAAAGAATTTTGAATTTGTC+GGG + Chr5:63043144-63043163 None:intergenic 20.0%
! ACGTAATCTACTTACATTAA+CGG + Chr5:63042814-63042833 None:intergenic 25.0%
! CTCTTTATCATAGGTTAAAT+GGG - Chr5:63042989-63043008 MsG0580027543.01.T01:CDS 25.0%
! GGAAATAATAATATAGCAGA+TGG + Chr5:63042770-63042789 None:intergenic 25.0%
! TCTCTTTATCATAGGTTAAA+TGG - Chr5:63042988-63043007 MsG0580027543.01.T01:CDS 25.0%
!! AAAGAATTTTGAATTTGTCG+GGG + Chr5:63043143-63043162 None:intergenic 25.0%
!! AGAGTTTTTGATGAAAACAA+CGG + Chr5:63042193-63042212 None:intergenic 25.0%
!!! GCTTTCTTATTTTGTTCTAA+TGG - Chr5:63042560-63042579 MsG0580027543.01.T01:intron 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + Chr5:63041718-63041737 None:intergenic 25.0%
AAACAGAGTAGACTAACTAA+AGG - Chr5:63042123-63042142 MsG0580027543.01.T01:CDS 30.0%
ACACAAACTGATACTAAATC+TGG - Chr5:63041991-63042010 MsG0580027543.01.T01:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - Chr5:63042517-63042536 MsG0580027543.01.T01:intron 30.0%
CCAAAATAGAGATTCTACTT+AGG + Chr5:63042862-63042881 None:intergenic 30.0%
! AATTCCGACATATATTTTCC+CGG + Chr5:63042217-63042236 None:intergenic 30.0%
! CCTAAGTAGAATCTCTATTT+TGG - Chr5:63042859-63042878 MsG0580027543.01.T01:intron 30.0%
! TAACTATAAGAGGTTTTCAG+CGG - Chr5:63042475-63042494 MsG0580027543.01.T01:intron 30.0%
!! TAGTTTAGGACTTTTTGTTG+TGG + Chr5:63043105-63043124 None:intergenic 30.0%
AAAGTTTACTACTTCCCACA+AGG - Chr5:63041730-63041749 MsG0580027543.01.T01:CDS 35.0%
AAATATATGTCGGAATTCGC+AGG - Chr5:63042220-63042239 MsG0580027543.01.T01:CDS 35.0%
AGAGTAGACTAACTAAAGGA+TGG - Chr5:63042127-63042146 MsG0580027543.01.T01:CDS 35.0%
AGATTTCCAACCAATCTTCA+TGG + Chr5:63042409-63042428 None:intergenic 35.0%
ATGAAAACAACGGAATCTTC+GGG + Chr5:63042183-63042202 None:intergenic 35.0%
ATTAACGGTCATGAGATATC+AGG + Chr5:63042799-63042818 None:intergenic 35.0%
CCAGATGGAAAAATAAACAG+TGG + Chr5:63043197-63043216 None:intergenic 35.0%
GATGAAAACAACGGAATCTT+CGG + Chr5:63042184-63042203 None:intergenic 35.0%
GATGGAAAAATAAACAGTGG+AGG + Chr5:63043194-63043213 None:intergenic 35.0%
TCATGAGATATCAGGAACAA+TGG + Chr5:63042791-63042810 None:intergenic 35.0%
TGAGAAATTAACTGAGAGTG+CGG - Chr5:63042272-63042291 MsG0580027543.01.T01:CDS 35.0%
! AACTATAAGAGGTTTTCAGC+GGG - Chr5:63042476-63042495 MsG0580027543.01.T01:intron 35.0%
! ATAAAGCTTGGCAGTTCTAA+AGG - Chr5:63043689-63043708 MsG0580027543.01.T01:CDS 35.0%
! ATGAAGATTGGTTGGAAATC+TGG - Chr5:63042408-63042427 MsG0580027543.01.T01:CDS 35.0%
! GAATTGGTGTAATTTTGTGG+TGG - Chr5:63042362-63042381 MsG0580027543.01.T01:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+CGG - Chr5:63042195-63042214 MsG0580027543.01.T01:CDS 35.0%
! TGGGAATTGGTGTAATTTTG+TGG - Chr5:63042359-63042378 MsG0580027543.01.T01:CDS 35.0%
! TTTTCATCAAAAACTCTGCC+GGG - Chr5:63042196-63042215 MsG0580027543.01.T01:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG + Chr5:63041704-63041723 None:intergenic 35.0%
!! ACTCTGTTTTGAGTAGGTAT+AGG + Chr5:63042112-63042131 None:intergenic 35.0%
!! CCACTGTTTATTTTTCCATC+TGG - Chr5:63043194-63043213 MsG0580027543.01.T01:CDS 35.0%
!! CTAAAGCTTGGCAGTTTTAA+AGG - Chr5:63043413-63043432 MsG0580027543.01.T01:CDS 35.0%
!! GAAGAACTCTGAGATAGTTT+AGG + Chr5:63043119-63043138 None:intergenic 35.0%
!! TAGTCTACTCTGTTTTGAGT+AGG + Chr5:63042118-63042137 None:intergenic 35.0%
ACCATCAAGAATGGTGATTG+TGG + Chr5:63041791-63041810 None:intergenic 40.0%
AGTGATACTTTGGCTGATAC+AGG - Chr5:63043458-63043477 MsG0580027543.01.T01:CDS 40.0%
AGTGGTACTTTGCTTGATAC+AGG - Chr5:63043263-63043282 MsG0580027543.01.T01:CDS 40.0%
ATGATGCCATGAAGATTGGT+TGG - Chr5:63042400-63042419 MsG0580027543.01.T01:CDS 40.0%
CATGTTCCAAATGACCTTGT+GGG + Chr5:63041747-63041766 None:intergenic 40.0%
GGAAAAATAAACAGTGGAGG+CGG + Chr5:63043191-63043210 None:intergenic 40.0%
GGATTTGGCTAACAAGAACA+TGG - Chr5:63042308-63042327 MsG0580027543.01.T01:CDS 40.0%
GTAGATGATGCCATGAAGAT+TGG - Chr5:63042396-63042415 MsG0580027543.01.T01:CDS 40.0%
GTGATACTTTGGCTGATACA+GGG - Chr5:63043459-63043478 MsG0580027543.01.T01:CDS 40.0%
GTGGTACTTTGCTTGATACA+GGG - Chr5:63043264-63043283 MsG0580027543.01.T01:CDS 40.0%
TCTGCCGGGAAAATATATGT+CGG - Chr5:63042210-63042229 MsG0580027543.01.T01:CDS 40.0%
TGAAAACAACGGAATCTTCG+GGG + Chr5:63042182-63042201 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG + Chr5:63041977-63041996 None:intergenic 40.0%
! ATTTTTCCATCTGGCATGCA+AGG - Chr5:63043203-63043222 MsG0580027543.01.T01:CDS 40.0%
! CTAAAGCTTGGCAGTTCTAA+AGG - Chr5:63043287-63043306 MsG0580027543.01.T01:CDS 40.0%
! TGGTTGGAAATCTGGAATGA+GGG - Chr5:63042416-63042435 MsG0580027543.01.T01:CDS 40.0%
! TTGGTTGGAAATCTGGAATG+AGG - Chr5:63042415-63042434 MsG0580027543.01.T01:CDS 40.0%
! TTGTGTTTTATCCCACAGCT+GGG - Chr5:63042340-63042359 MsG0580027543.01.T01:CDS 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG + Chr5:63041703-63041722 None:intergenic 40.0%
!!! AGTTTTAAAGGCCCTGACTT+GGG - Chr5:63043425-63043444 MsG0580027543.01.T01:CDS 40.0%
!!! TAATTTTGTGGTGGATGCCA+AGG - Chr5:63042371-63042390 MsG0580027543.01.T01:CDS 40.0%
AAACTATGGCAGAAATGCGC+TGG - Chr5:63041676-63041695 MsG0580027543.01.T01:CDS 45.0%
ACCTTAAGCATGCGCCAATT+AGG + Chr5:63042534-63042553 None:intergenic 45.0%
AGCGCATTTCTGCCATAGTT+TGG + Chr5:63041677-63041696 None:intergenic 45.0%
AGGAGGTTCTTGGTCTGTTA+CGG + Chr5:63041905-63041924 None:intergenic 45.0%
AGTAGAGACGATGCATGGAA+TGG + Chr5:63041824-63041843 None:intergenic 45.0%
ATAAACAGTGGAGGCGGTAA+TGG + Chr5:63043185-63043204 None:intergenic 45.0%
ATGGTGATTGTGGCAGCATT+AGG + Chr5:63041781-63041800 None:intergenic 45.0%
ATTACACCAATTCCCAGCTG+TGG + Chr5:63042355-63042374 None:intergenic 45.0%
CACAGAGTCGAGTGATACTT+TGG - Chr5:63043655-63043674 MsG0580027543.01.T01:CDS 45.0%
CATGGCATCATCTACAACCT+TGG + Chr5:63042391-63042410 None:intergenic 45.0%
CCTGATACAAGGCTAAAGCT+TGG - Chr5:63043344-63043363 MsG0580027543.01.T01:CDS 45.0%
CCTTAAGCATGCGCCAATTA+GGG + Chr5:63042533-63042552 None:intergenic 45.0%
CTACTTCCCACAAGGTCATT+TGG - Chr5:63041738-63041757 MsG0580027543.01.T01:CDS 45.0%
CTTGATACAGGGCTAAAGCT+TGG - Chr5:63043275-63043294 MsG0580027543.01.T01:CDS 45.0%
GCATGTTCCAAATGACCTTG+TGG + Chr5:63041748-63041767 None:intergenic 45.0%
GCCACAATCACCATTCTTGA+TGG - Chr5:63041787-63041806 MsG0580027543.01.T01:CDS 45.0%
GCTGATACAGAGCTAAAGCT+TGG - Chr5:63043401-63043420 MsG0580027543.01.T01:CDS 45.0%
GCTGATACAGGGATAAAGCT+TGG - Chr5:63043677-63043696 MsG0580027543.01.T01:CDS 45.0%
GCTGATACAGTGCTAAAGCT+TGG - Chr5:63043746-63043765 MsG0580027543.01.T01:CDS 45.0%
GTATAGGTGTAGACTTTGCC+TGG + Chr5:63042096-63042115 None:intergenic 45.0%
GTGGTACTTTGCCTGATACA+AGG - Chr5:63043333-63043352 MsG0580027543.01.T01:CDS 45.0%
TCTTGAGACTTCTGCAACTC+TGG + Chr5:63042054-63042073 None:intergenic 45.0%
TGGACGGTAACCATCAAGAA+TGG + Chr5:63041800-63041819 None:intergenic 45.0%
TGGTGATTGTGGCAGCATTA+GGG + Chr5:63041780-63041799 None:intergenic 45.0%
TGTTGTGGAGATGTTGACAG+TGG + Chr5:63043090-63043109 None:intergenic 45.0%
TGTTTCCATCGTCGTCTTCT+TGG + Chr5:63041945-63041964 None:intergenic 45.0%
TTACACCAATTCCCAGCTGT+GGG + Chr5:63042354-63042373 None:intergenic 45.0%
TTTATCCCACAGCTGGGAAT+TGG - Chr5:63042346-63042365 MsG0580027543.01.T01:CDS 45.0%
! ATAAGAGGTTTTCAGCGGGA+GGG - Chr5:63042480-63042499 MsG0580027543.01.T01:intron 45.0%
! ATCAAGTCTGTGCATTGACG+AGG + Chr5:63042027-63042046 None:intergenic 45.0%
! ATTAACTGAGAGTGCGGTCT+TGG - Chr5:63042278-63042297 MsG0580027543.01.T01:CDS 45.0%
! GGTAATGGTGTCAGTGTACT+TGG + Chr5:63043170-63043189 None:intergenic 45.0%
! GTGTTGAGTTGTTGCCTCAT+AGG - Chr5:63043780-63043799 MsG0580027543.01.T01:CDS 45.0%
! GTTGTGTTTTATCCCACAGC+TGG - Chr5:63042339-63042358 MsG0580027543.01.T01:CDS 45.0%
! TATAAGAGGTTTTCAGCGGG+AGG - Chr5:63042479-63042498 MsG0580027543.01.T01:intron 45.0%
! TTAACTGAGAGTGCGGTCTT+GGG - Chr5:63042279-63042298 MsG0580027543.01.T01:CDS 45.0%
!! AGTTCTAAAGGTGCGGACTT+GGG - Chr5:63043494-63043513 MsG0580027543.01.T01:CDS 45.0%
!! CCAAGCTTTAGCCTTGTATC+AGG + Chr5:63043347-63043366 None:intergenic 45.0%
!!! CAGTTTTAAAGGCCCTGACT+TGG - Chr5:63043424-63043443 MsG0580027543.01.T01:CDS 45.0%
AACTTCCCTGCAGCTGAAAG+AGG - Chr5:63042249-63042268 MsG0580027543.01.T01:CDS 50.0%
ACAGTGGAGGAGGAAGTTGA+TGG + Chr5:63043074-63043093 None:intergenic 50.0%
ATGCAAGGAGTCAGGCAAGA+AGG - Chr5:63043218-63043237 MsG0580027543.01.T01:CDS 50.0%
CAGTTTGGCAAAGACCTCGT+CGG + Chr5:63041872-63041891 None:intergenic 50.0%
CCAACAGTAGAGACGATGCA+TGG + Chr5:63041829-63041848 None:intergenic 50.0%
CCATGCATCGTCTCTACTGT+TGG - Chr5:63041826-63041845 MsG0580027543.01.T01:CDS 50.0%
CCCTAATTGGCGCATGCTTA+AGG - Chr5:63042530-63042549 MsG0580027543.01.T01:intron 50.0%
CGATGCATGGAATGGATGGA+CGG + Chr5:63041816-63041835 None:intergenic 50.0%
CGCAGAGTCGACTGATACTT+TGG - Chr5:63043379-63043398 MsG0580027543.01.T01:CDS 50.0%
CGCAGAGTCGAGTGATACTT+TGG - Chr5:63043448-63043467 MsG0580027543.01.T01:CDS 50.0%
CTTGGCTCATTGGACCTATG+AGG + Chr5:63043797-63043816 None:intergenic 50.0%
CTTGGTCTGTTACGGGAGTT+AGG + Chr5:63041897-63041916 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + Chr5:63041820-63041839 None:intergenic 50.0%
GAGGGCAAAGCTTCGTTTGA+TGG - Chr5:63042434-63042453 MsG0580027543.01.T01:CDS 50.0%
GCTGATACAGGGCTAAAGCT+TGG - Chr5:63043470-63043489 MsG0580027543.01.T01:CDS 50.0%
GGAGGTTCTTGGTCTGTTAC+GGG + Chr5:63041904-63041923 None:intergenic 50.0%
GGGTAAACTTCAACCTCCCA+TGG + Chr5:63043053-63043072 None:intergenic 50.0%
GGTGATTGTGGCAGCATTAG+GGG + Chr5:63041779-63041798 None:intergenic 50.0%
GGTGGTTTCTGCGGGATATT+AGG + Chr5:63043020-63043039 None:intergenic 50.0%
GTCTCTGTCACTGATCTTCC+AGG - Chr5:63042075-63042094 MsG0580027543.01.T01:CDS 50.0%
TACGGGAGTTAGGAGCAGTT+TGG + Chr5:63041887-63041906 None:intergenic 50.0%
TCTCACCTCTTTCAGCTGCA+GGG + Chr5:63042257-63042276 None:intergenic 50.0%
TGTGGAGATGTTGACAGTGG+AGG + Chr5:63043087-63043106 None:intergenic 50.0%
TTCTCACCTCTTTCAGCTGC+AGG + Chr5:63042258-63042277 None:intergenic 50.0%
! AACCTCCCATGGATTGACCA+GGG + Chr5:63043042-63043061 None:intergenic 50.0%
! CAGTGGAGGAGGAAGTTGAT+GGG + Chr5:63043073-63043092 None:intergenic 50.0%
! GACTTGTTTGCAGAGTCGAG+TGG - Chr5:63043314-63043333 MsG0580027543.01.T01:CDS 50.0%
! TAAGAGGTTTTCAGCGGGAG+GGG - Chr5:63042481-63042500 MsG0580027543.01.T01:intron 50.0%
!! AAGGTGCTGACTTGTTCGCA+GGG - Chr5:63043237-63043256 MsG0580027543.01.T01:CDS 50.0%
!! CAGTTCTAAAGGTGCGGACT+TGG - Chr5:63043493-63043512 MsG0580027543.01.T01:CDS 50.0%
!! GCTTGGCAGTTCTAAAGGTG+CGG - Chr5:63043487-63043506 MsG0580027543.01.T01:CDS 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG + Chr5:63043807-63043826 None:intergenic 50.0%
AACCACCCCTGGTCAATCCA+TGG - Chr5:63043033-63043052 MsG0580027543.01.T01:CDS 55.0%
ACCACCCCTGGTCAATCCAT+GGG - Chr5:63043034-63043053 MsG0580027543.01.T01:CDS 55.0%
CATCTGGCATGCAAGGAGTC+AGG - Chr5:63043210-63043229 MsG0580027543.01.T01:CDS 55.0%
CGCAGAGGCGAGTGATACTT+TGG - Chr5:63043586-63043605 MsG0580027543.01.T01:CDS 55.0%
CTGACTCCTTGCATGCCAGA+TGG + Chr5:63043212-63043231 None:intergenic 55.0%
GGAGATGTTGACAGTGGAGG+AGG + Chr5:63043084-63043103 None:intergenic 55.0%
TCTTCTTGGCCGTGAACCAC+AGG + Chr5:63041931-63041950 None:intergenic 55.0%
TGTTGATCCTCACACCGACG+AGG - Chr5:63041855-63041874 MsG0580027543.01.T01:CDS 55.0%
TTGACCAGGGGTGGTTTCTG+CGG + Chr5:63043029-63043048 None:intergenic 55.0%
! ACCTCCCATGGATTGACCAG+GGG + Chr5:63043041-63043060 None:intergenic 55.0%
! CAACCTCCCATGGATTGACC+AGG + Chr5:63043043-63043062 None:intergenic 55.0%
! GGTCTTGGGTGCAGTGGATT+TGG - Chr5:63042293-63042312 MsG0580027543.01.T01:CDS 55.0%
!! GAAGGTGCTGACTTGTTCGC+AGG - Chr5:63043236-63043255 MsG0580027543.01.T01:CDS 55.0%
ACCCCTGGTCAATCCATGGG+AGG - Chr5:63043037-63043056 MsG0580027543.01.T01:CDS 60.0%
CACGGCCAAGAAGACGACGA+TGG - Chr5:63041937-63041956 MsG0580027543.01.T01:CDS 60.0%
CCAAGAACCTCCTCCTCCTG+TGG - Chr5:63041912-63041931 MsG0580027543.01.T01:CDS 60.0%
CCACAGGAGGAGGAGGTTCT+TGG + Chr5:63041915-63041934 None:intergenic 60.0%
CCTCCTCCTCCTGTGGTTCA+CGG - Chr5:63041919-63041938 MsG0580027543.01.T01:CDS 60.0%
GCAAAGACCTCGTCGGTGTG+AGG + Chr5:63041865-63041884 None:intergenic 60.0%
TATCCCGCAGAAACCACCCC+TGG - Chr5:63043022-63043041 MsG0580027543.01.T01:CDS 60.0%
TCGACTCTGCGCCCAAGTCA+GGG + Chr5:63043439-63043458 None:intergenic 60.0%
TCTTGGCCGTGAACCACAGG+AGG + Chr5:63041928-63041947 None:intergenic 60.0%
TGACCAGGGGTGGTTTCTGC+GGG + Chr5:63043028-63043047 None:intergenic 60.0%
! GACTTGTTCGCAGGGTCGAG+TGG - Chr5:63043245-63043264 MsG0580027543.01.T01:CDS 60.0%
! TCCCATGGATTGACCAGGGG+TGG + Chr5:63043038-63043057 None:intergenic 60.0%
CCGTGAACCACAGGAGGAGG+AGG + Chr5:63041922-63041941 None:intergenic 65.0%
CTCGACTCTGCGCCCAAGTC+AGG + Chr5:63043440-63043459 None:intergenic 65.0%
GAGTGCGGTCTTGGGTGCAG+TGG - Chr5:63042287-63042306 MsG0580027543.01.T01:CDS 65.0%
TGGCCGTGAACCACAGGAGG+AGG + Chr5:63041925-63041944 None:intergenic 65.0%
AGGTGCGGACTTGGGCGCAG+AGG - Chr5:63043502-63043521 MsG0580027543.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 63041667 63043826 63041667 ID=MsG0580027543.01;Name=MsG0580027543.01
Chr5 mRNA 63041667 63043826 63041667 ID=MsG0580027543.01.T01;Parent=MsG0580027543.01;Name=MsG0580027543.01.T01;_AED=0.53;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|569
Chr5 exon 63042942 63043826 63042942 ID=MsG0580027543.01.T01:exon:26507;Parent=MsG0580027543.01.T01
Chr5 exon 63041667 63042491 63041667 ID=MsG0580027543.01.T01:exon:26508;Parent=MsG0580027543.01.T01
Chr5 CDS 63042942 63043826 63042942 ID=MsG0580027543.01.T01:cds;Parent=MsG0580027543.01.T01
Chr5 CDS 63041667 63042491 63041667 ID=MsG0580027543.01.T01:cds;Parent=MsG0580027543.01.T01
Gene Sequence

>MsG0580027543.01.T01

ATGGACTCCAAACTATGGCAGAAATGCGCTGGTCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACAAGGTCATTTGGAACATGCATGTTCTTCCCCTAATGCTGCCACAATCACCATTCTTGATGGTTACCGTCCATCCATTCCATGCATCGTCTCTACTGTTGGTCTCTTTGTTGATCCTCACACCGACGAGGTCTTTGCCAAACTGCTCCTAACTCCCGTAACAGACCAAGAACCTCCTCCTCCTGTGGTTCACGGCCAAGAAGACGACGATGGAAACAATCTTGTTTCCTATGTCAAGACTCTCACACAAACTGATACTAAATCTGGATTCAATATGCCTCGTCAATGCACAGACTTGATCTTTCCAGAGTTGCAGAAGTCTCAAGAAGTCTCTGTCACTGATCTTCCAGGCAAAGTCTACACCTATACCTACTCAAAACAGAGTAGACTAACTAAAGGATGGTTTAATTTTATAAGTGAGAAGAAACTCGTCCCCGAAGATTCCGTTGTTTTCATCAAAAACTCTGCCGGGAAAATATATGTCGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAAGAGGTGAGAAATTAACTGAGAGTGCGGTCTTGGGTGCAGTGGATTTGGCTAACAAGAACATGGCGTTTGAAGTTGTGTTTTATCCCACAGCTGGGAATTGGTGTAATTTTGTGGTGGATGCCAAGGTTGTAGATGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGGGCAAAGCTTCGTTTGATGGAACCTGAATCTTCAAATTTAACTATAAGAGGTTTTCAGCGGGAGGGGACAATCTCTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTAAGGTTAAATGGGATGAACCTAATATCCCGCAGAAACCACCCCTGGTCAATCCATGGGAGGTTGAAGTTTACCCATCAACTTCCTCCTCCACTGTCAACATCTCCACAACAAAAAGTCCTAAACTATCTCAGAGTTCTTCTCCCCGACAAATTCAAAATTCTTTAATGCCAAGTACACTGACACCATTACCGCCTCCACTGTTTATTTTTCCATCTGGCATGCAAGGAGTCAGGCAAGAAGGTGCTGACTTGTTCGCAGGGTCGAGTGGTACTTTGCTTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGATGCTGACTTGTTTGCAGAGTCGAGTGGTACTTTGCCTGATACAAGGCTAAAGCTTGGCAGTTCTAAAGGCGCAGAGTCGACTGATACTTTGGCTGATACAGAGCTAAAGCTTGGCAGTTTTAAAGGCCCTGACTTGGGCGCAGAGTCGAGTGATACTTTGGCTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCGCAGAGTCGAGTGATACTTTGGCTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCGCAGAGGCGAGTGATACTTTGGCTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCACAGAGTCGAGTGATACTTTGGCTGATACAGGGATAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCGCAGAGTCGAGTGATACTTTGGCTGATACAGTGCTAAAGCTTGGCAGTTCTAAAGGTGTTGAGTTGTTGCCTCATAGGTCCAATGAGCCAAGCACCAAGTGA
Protein sequence

>MsG0580027543.01.T01

MDSKLWQKCAGPDVKIPKIHSKVYYFPQGHLEHACSSPNAATITILDGYRPSIPCIVSTVGLFVDPHTDEVFAKLLLTPVTDQEPPPPVVHGQEDDDGNNLVSYVKTLTQTDTKSGFNMPRQCTDLIFPELQKSQEVSVTDLPGKVYTYTYSKQSRLTKGWFNFISEKKLVPEDSVVFIKNSAGKIYVGIRRNTNFPAAERGEKLTESAVLGAVDLANKNMAFEVVFYPTAGNWCNFVVDAKVVDDAMKIGWKSGMRAKLRLMEPESSNLTIRGFQREGTISNDSSNVPNWRMLKVKWDEPNIPQKPPLVNPWEVEVYPSTSSSTVNISTTKSPKLSQSSSPRQIQNSLMPSTLTPLPPPLFIFPSGMQGVRQEGADLFAGSSGTLLDTGLKLGSSKDADLFAESSGTLPDTRLKLGSSKGAESTDTLADTELKLGSFKGPDLGAESSDTLADTGLKLGSSKGADLGAESSDTLADTGLKLGSSKGADLGAEASDTLADTGLKLGSSKGADLGTESSDTLADTGIKLGSSKGADLGAESSDTLADTVLKLGSSKGVELLPHRSNEPSTK*