AlfalfaGEDB | MsG0580027729.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027729.01

KEGG: -
GO: GO:0000982 GO:0000987 GO:0003677 GO:0045944 GO:0046983
PFAM: PF00319

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027729.01.T01	XP_003605525.1	75.217	230	57	0	1	230	1	230	3.73E-125	364

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027729.01.T01	Q9FJK3	52.903	155	72	1	1	155	1	154	2.35E-45	156

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027729.01.T01	G7JN85	75.217	230	57	0	1	230	1	230	1.78e-125	364

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TFs/TRs:

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Gene ID	Type	Classification
MsG0580027729.01.T01	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027729.01.T01	MTR_4g032620	75.217	230	57	0	1	230	1	230	4.51e-129	364
MsG0580027729.01.T01	MTR_8g036130	61.382	246	79	3	1	230	1	246	7.83e-96	280
MsG0580027729.01.T01	MTR_4g031910	52.743	237	96	2	1	230	1	228	1.47e-74	226
MsG0580027729.01.T01	MTR_4g032290	53.498	243	87	5	1	230	1	230	7.23e-71	216
MsG0580027729.01.T01	MTR_3g065100	50.417	240	105	5	1	228	1	238	1.25e-64	201
MsG0580027729.01.T01	MTR_3g466980	44.068	236	108	7	1	227	1	221	2.86e-57	181
MsG0580027729.01.T01	MTR_4g032260	63.057	157	58	0	1	157	1	157	3.74e-57	179
MsG0580027729.01.T01	MTR_2g035610	70.248	121	36	0	1	121	1	121	5.51e-50	159
MsG0580027729.01.T01	MTR_3g466830	44.785	163	82	3	3	160	2	161	6.22e-44	145
MsG0580027729.01.T01	MTR_5g047580	44.231	156	85	2	1	156	1	154	1.28e-42	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027729.01.T01	AT5G48670	52.903	155	72	1	1	155	1	154	2.40e-46	156
MsG0580027729.01.T01	AT1G65300	41.243	177	97	2	3	173	2	177	7.28e-39	136
MsG0580027729.01.T01	AT5G26630	40.284	211	124	2	1	210	1	210	1.28e-37	131
MsG0580027729.01.T01	AT1G65330	39.247	186	108	2	3	184	2	186	2.86e-36	129
MsG0580027729.01.T01	AT3G05860	42.000	150	86	1	1	150	1	149	6.30e-36	126
MsG0580027729.01.T01	AT5G27960	42.484	153	87	1	4	156	2	153	7.24e-36	129
MsG0580027729.01.T01	AT5G26650	43.137	153	86	1	4	156	2	153	8.09e-36	130
MsG0580027729.01.T01	AT3G05860	39.752	161	96	1	1	161	1	160	3.57e-35	125
MsG0580027729.01.T01	AT3G05860	42.000	150	86	1	1	150	1	149	4.06e-35	125
MsG0580027729.01.T01	AT5G27810	50.538	93	46	0	29	121	1	93	5.87e-30	108

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Find 51 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGCAATGATTGGTCA+TGG	0.627083	5:-67533859	MsG0580027729.01.T01:CDS
AACCTACAATAAAAGGCAGA+AGG	0.564320	5:-67534337	MsG0580027729.01.T01:CDS
AAGAGAAACTGAAGACACAA+AGG	0.673063	5:-67534101	MsG0580027729.01.T01:CDS
AAGAGACTTATATGATACAA+AGG	0.581969	5:-67534137	MsG0580027729.01.T01:CDS
AATTCAGAACAATGTCTAAG+TGG	0.549030	5:-67534185	MsG0580027729.01.T01:CDS
AATTGAAGGACATTAGTAGA+AGG	0.455635	5:-67533957	MsG0580027729.01.T01:CDS
ACATCCAATTCAATTCCAAA+TGG	0.514429	5:-67533908	MsG0580027729.01.T01:CDS
ACCTACAATAAAAGGCAGAA+GGG	0.621576	5:-67534336	MsG0580027729.01.T01:CDS
AGAGACTTATATGATACAAA+GGG	0.576379	5:-67534136	MsG0580027729.01.T01:CDS
AGCACTGTATGGACTCCCCG+TGG	0.777500	5:+67534213	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTCAGAACAATGTCTAAG+TGG	-	Chr5:67533928-67533947	MsG0580027729.01.T01:CDS	30.0%
AATTGAAGGACATTAGTAGA+AGG	-	Chr5:67534156-67534175	MsG0580027729.01.T01:CDS	30.0%
ACATCCAATTCAATTCCAAA+TGG	-	Chr5:67534205-67534224	MsG0580027729.01.T01:CDS	30.0%
ATGTTTCAATGTCTTTGTGA+AGG	-	Chr5:67534068-67534087	MsG0580027729.01.T01:CDS	30.0%
ATTCAGAACAATGTCTAAGT+GGG	-	Chr5:67533929-67533948	MsG0580027729.01.T01:CDS	30.0%
GATGAAAAAATGGCAATGAT+TGG	-	Chr5:67534248-67534267	MsG0580027729.01.T01:CDS	30.0%
AACCTACAATAAAAGGCAGA+AGG	-	Chr5:67533776-67533795	MsG0580027729.01.T01:CDS	35.0%
AAGAGAAACTGAAGACACAA+AGG	-	Chr5:67534012-67534031	MsG0580027729.01.T01:CDS	35.0%
ACCTACAATAAAAGGCAGAA+GGG	-	Chr5:67533777-67533796	MsG0580027729.01.T01:CDS	35.0%
AGTAGATTGGGCAAAAATCT+AGG	+	Chr5:67534389-67534408	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	67533720	67534415	67533720	ID=MsG0580027729.01;Name=MsG0580027729.01
Chr5	mRNA	67533720	67534415	67533720	ID=MsG0580027729.01.T01;Parent=MsG0580027729.01;Name=MsG0580027729.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|231
Chr5	exon	67533720	67534415	67533720	ID=MsG0580027729.01.T01:exon:44189;Parent=MsG0580027729.01.T01
Chr5	CDS	67533720	67534415	67533720	ID=MsG0580027729.01.T01:cds;Parent=MsG0580027729.01.T01

Gene Sequence

>MsG0580027729.01.T01

ATGACTCGGAAAAAGGTGAAACTTGCTTTCATTGTCAATGATGCTTCTAGAAAAACAACCTACAATAAAAGGCAGAAGGGTCTATTGAAGAAGGTTTATGAACTAAGCACTCTATGTGGAGTTGAAGCTTGTGCCATTGTCTATGGACCATATGAGCCTCAACTGGAGATCTGGCCATCACCACGGGGAGTCCATACAGTGCTCTCAAAATTCAGAACAATGTCTAAGTGGGAGAAGAGCCAGAAAATGGTGAACCAAGAGACTTATATGATACAAAGGGTTCTGAATGCCAAAGAGAAACTGAAGACACAAAGGCATGACAACTATGAGAAAGAGATGGTTATGCTCATGTTTCAATGTCTTTGTGAAGGCAAATTAGTGCAGAATAATATCTCCTTGGTTGATTCAAAACATCTTTGTTGGTTGATTTACCAAAAATTGAAGGACATTAGTAGAAGGTTGGAAGCAGGGGTCAGTAATAGTAAACATCCAATTCAATTCCAAATGGCACCACCATTGACATCCAAGGATGAAAAAATGGCAATGATTGGTCATGGCTATGTTGGGATGACTAGGAACAATGGTGACATCATGGAAAGTCAAATTTTCATGGGATTGATGATGAATGATAAAGGAGACGAGACAGTACCCTTTGGCGAGGTCGATCCTAGATTTTTGCCCAATCTACTTCTTTGA

Protein sequence

>MsG0580027729.01.T01

MTRKKVKLAFIVNDASRKTTYNKRQKGLLKKVYELSTLCGVEACAIVYGPYEPQLEIWPSPRGVHTVLSKFRTMSKWEKSQKMVNQETYMIQRVLNAKEKLKTQRHDNYEKEMVMLMFQCLCEGKLVQNNISLVDSKHLCWLIYQKLKDISRRLEAGVSNSKHPIQFQMAPPLTSKDEKMAMIGHGYVGMTRNNGDIMESQIFMGLMMNDKGDETVPFGEVDPRFLPNLLL*