Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028042.01.T01 | XP_003615578.1 | 95.714 | 140 | 6 | 0 | 1 | 140 | 1 | 140 | 6.79E-97 | 291 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028042.01.T01 | Q8S9H7 | 63.83 | 141 | 45 | 3 | 5 | 140 | 1 | 140 | 1.62E-54 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028042.01.T01 | G7K698 | 95.714 | 140 | 6 | 0 | 1 | 140 | 1 | 140 | 3.24e-97 | 291 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580028042.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028042.01.T01 | MTR_5g069710 | 95.714 | 140 | 6 | 0 | 1 | 140 | 1 | 140 | 8.22e-101 | 291 |
| MsG0580028042.01.T01 | MTR_3g462790 | 70.323 | 155 | 31 | 4 | 1 | 140 | 1 | 155 | 1.38e-72 | 220 |
| MsG0580028042.01.T01 | MTR_7g067080 | 67.361 | 144 | 41 | 2 | 1 | 140 | 7 | 148 | 1.54e-64 | 199 |
| MsG0580028042.01.T01 | MTR_5g081860 | 52.555 | 137 | 60 | 2 | 4 | 140 | 2 | 133 | 1.59e-45 | 149 |
| MsG0580028042.01.T01 | MTR_8g063600 | 37.815 | 119 | 67 | 2 | 22 | 140 | 3 | 114 | 1.27e-22 | 90.5 |
| MsG0580028042.01.T01 | MTR_1g033600 | 37.795 | 127 | 62 | 3 | 26 | 140 | 9 | 130 | 9.29e-22 | 85.1 |
| MsG0580028042.01.T01 | MTR_1g111830 | 39.316 | 117 | 62 | 2 | 27 | 140 | 22 | 132 | 1.39e-21 | 87.0 |
| MsG0580028042.01.T01 | MTR_1g022290 | 49.296 | 71 | 36 | 0 | 26 | 96 | 4 | 74 | 4.89e-21 | 81.6 |
| MsG0580028042.01.T01 | MTR_5g020170 | 44.000 | 75 | 42 | 0 | 17 | 91 | 7 | 81 | 9.37e-21 | 81.3 |
| MsG0580028042.01.T01 | MTR_8g077380 | 51.389 | 72 | 34 | 1 | 25 | 96 | 1 | 71 | 1.31e-20 | 80.1 |
| MsG0580028042.01.T01 | MTR_8g077390 | 51.389 | 72 | 34 | 1 | 25 | 96 | 1 | 71 | 1.31e-20 | 80.1 |
| MsG0580028042.01.T01 | MTR_8g077360 | 51.389 | 72 | 34 | 1 | 25 | 96 | 1 | 71 | 1.43e-20 | 80.1 |
| MsG0580028042.01.T01 | MTR_3g116720 | 52.308 | 65 | 31 | 0 | 29 | 93 | 16 | 80 | 2.29e-20 | 79.7 |
| MsG0580028042.01.T01 | MTR_3g111880 | 49.351 | 77 | 38 | 1 | 26 | 101 | 4 | 80 | 6.27e-20 | 79.0 |
| MsG0580028042.01.T01 | MTR_6g004250 | 47.222 | 72 | 38 | 0 | 26 | 97 | 7 | 78 | 5.58e-19 | 75.9 |
| MsG0580028042.01.T01 | MTR_5g020540 | 49.206 | 63 | 31 | 1 | 27 | 89 | 2 | 63 | 3.97e-17 | 70.9 |
| MsG0580028042.01.T01 | MTR_8g063870 | 43.662 | 71 | 40 | 0 | 23 | 93 | 2 | 72 | 5.09e-17 | 75.5 |
| MsG0580028042.01.T01 | MTR_8g077420 | 49.231 | 65 | 32 | 1 | 25 | 89 | 1 | 64 | 5.30e-17 | 70.9 |
| MsG0580028042.01.T01 | MTR_7g089210 | 53.333 | 60 | 28 | 0 | 32 | 91 | 15 | 74 | 1.05e-16 | 70.5 |
| MsG0580028042.01.T01 | MTR_3g013440 | 50.746 | 67 | 32 | 1 | 26 | 91 | 7 | 73 | 2.81e-16 | 69.3 |
| MsG0580028042.01.T01 | MTR_4g111975 | 34.028 | 144 | 58 | 4 | 29 | 140 | 8 | 146 | 2.98e-16 | 73.6 |
| MsG0580028042.01.T01 | MTR_3g111920 | 44.444 | 63 | 35 | 0 | 29 | 91 | 3 | 65 | 3.58e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028042.01.T01 | AT2G38090 | 56.494 | 154 | 48 | 3 | 1 | 140 | 1 | 149 | 2.13e-55 | 175 |
| MsG0580028042.01.T01 | AT5G58900 | 56.000 | 150 | 51 | 5 | 5 | 140 | 1 | 149 | 2.44e-46 | 152 |
| MsG0580028042.01.T01 | AT5G05790 | 52.672 | 131 | 54 | 4 | 14 | 140 | 13 | 139 | 2.05e-38 | 131 |
| MsG0580028042.01.T01 | AT5G05790 | 52.672 | 131 | 54 | 4 | 14 | 140 | 13 | 139 | 2.05e-38 | 131 |
| MsG0580028042.01.T01 | AT3G11280 | 58.036 | 112 | 38 | 2 | 29 | 140 | 33 | 135 | 5.58e-38 | 130 |
| MsG0580028042.01.T01 | AT3G11280 | 58.036 | 112 | 38 | 2 | 29 | 140 | 33 | 135 | 5.58e-38 | 130 |
| MsG0580028042.01.T01 | AT5G01200 | 53.957 | 139 | 47 | 6 | 16 | 139 | 17 | 153 | 3.40e-37 | 128 |
| MsG0580028042.01.T01 | AT1G75250 | 58.462 | 65 | 27 | 0 | 29 | 93 | 12 | 76 | 8.57e-23 | 87.0 |
| MsG0580028042.01.T01 | AT1G75250 | 58.462 | 65 | 27 | 0 | 29 | 93 | 12 | 76 | 9.53e-23 | 86.3 |
| MsG0580028042.01.T01 | AT2G21650 | 53.731 | 67 | 31 | 0 | 29 | 95 | 14 | 80 | 4.16e-19 | 77.0 |
| MsG0580028042.01.T01 | AT4G39250 | 49.231 | 65 | 33 | 0 | 29 | 93 | 14 | 78 | 4.77e-18 | 74.3 |
| MsG0580028042.01.T01 | AT1G19510 | 46.914 | 81 | 36 | 2 | 32 | 111 | 15 | 89 | 3.06e-17 | 72.4 |
| MsG0580028042.01.T01 | AT5G08520 | 35.345 | 116 | 70 | 3 | 29 | 140 | 12 | 126 | 3.81e-17 | 76.3 |
| MsG0580028042.01.T01 | AT1G49010 | 37.762 | 143 | 62 | 5 | 23 | 140 | 2 | 142 | 7.49e-17 | 75.5 |
| MsG0580028042.01.T01 | AT5G23650 | 28.986 | 138 | 72 | 3 | 18 | 140 | 2 | 128 | 2.91e-14 | 68.6 |
| MsG0580028042.01.T01 | AT2G18328 | 52.542 | 59 | 28 | 0 | 33 | 91 | 15 | 73 | 4.12e-14 | 63.5 |
| MsG0580028042.01.T01 | AT5G04760 | 34.783 | 115 | 65 | 2 | 26 | 140 | 3 | 107 | 1.05e-13 | 65.9 |
| MsG0580028042.01.T01 | AT4G36570 | 58.140 | 43 | 18 | 0 | 29 | 71 | 12 | 54 | 8.85e-13 | 59.7 |
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGATTGGGTGAGCAGTTC+TGG | 0.273897 | 5:-73664102 | MsG0580028042.01.T01:CDS |
| CCTTTCTTCCTTTCCTGCTC+AGG | 0.322349 | 5:+73664030 | None:intergenic |
| CTCACCCAATCTAAGGTAAA+AGG | 0.326033 | 5:+73664111 | None:intergenic |
| TCTGGTTATGATGAATTTAG+AGG | 0.373350 | 5:-73664084 | MsG0580028042.01.T01:CDS |
| GCTGGGTTGATTCCAGTTCC+TGG | 0.382171 | 5:-73664153 | MsG0580028042.01.T01:CDS |
| ACATCACCGACCGTCTTTCC+TGG | 0.388704 | 5:+73664222 | None:intergenic |
| AGAACTGCTCACCCAATCTA+AGG | 0.399431 | 5:+73664104 | None:intergenic |
| TGATAAGATGAAGTGGGAAA+TGG | 0.405855 | 5:-73664406 | None:intergenic |
| TATGATGAATTTAGAGGAAG+TGG | 0.409816 | 5:-73664078 | MsG0580028042.01.T01:CDS |
| TGATGTGATGAGACAATATA+AGG | 0.419767 | 5:-73664205 | MsG0580028042.01.T01:CDS |
| GTTATCTTCCATACACCAAT+TGG | 0.440080 | 5:+73664350 | None:intergenic |
| GATGTCTGTAATATAGAAGC+TGG | 0.449562 | 5:-73664171 | MsG0580028042.01.T01:CDS |
| TTGTGTTCTTCTTCAGTCCA+TGG | 0.457416 | 5:+73664003 | None:intergenic |
| CATACACACAAAACTCCAAT+TGG | 0.463612 | 5:-73664365 | MsG0580028042.01.T01:CDS |
| GGAGTATTATAACCAGGAAC+TGG | 0.465713 | 5:+73664141 | None:intergenic |
| ACAAAACTCCAATTGGTGTA+TGG | 0.484625 | 5:-73664358 | MsG0580028042.01.T01:CDS |
| TTAGAGCTCCTGAGCAGGAA+AGG | 0.515723 | 5:-73664038 | MsG0580028042.01.T01:CDS |
| TCTAAGGTAAAAGGTGAAGT+AGG | 0.517348 | 5:+73664120 | None:intergenic |
| CCTGAGCAGGAAAGGAAGAA+AGG | 0.521387 | 5:-73664030 | MsG0580028042.01.T01:CDS |
| TTTAGTTAGAGCTCCTGAGC+AGG | 0.523673 | 5:-73664043 | MsG0580028042.01.T01:CDS |
| GAAAACTTGATAAGATGAAG+TGG | 0.527680 | 5:-73664413 | None:intergenic |
| GCTACTTTATGCCATCGATC+CGG | 0.541949 | 5:+73664255 | None:intergenic |
| ATAAAGATACGCCGGATCGA+TGG | 0.544701 | 5:-73664266 | MsG0580028042.01.T01:CDS |
| CTCTTCTGGAGTCCATTGTG+TGG | 0.588727 | 5:+73664323 | None:intergenic |
| AAAGTAGCTGAAATGATACC+AGG | 0.595280 | 5:-73664240 | MsG0580028042.01.T01:CDS |
| AAAACTTGATAAGATGAAGT+GGG | 0.612600 | 5:-73664412 | None:intergenic |
| TTTGTGTGTATGATGCAGGA+GGG | 0.617317 | 5:+73664376 | None:intergenic |
| AAAGGAAGAAAGGAGTGCCA+TGG | 0.621552 | 5:-73664020 | MsG0580028042.01.T01:CDS |
| TGAAATGATACCAGGAAAGA+CGG | 0.622184 | 5:-73664232 | MsG0580028042.01.T01:CDS |
| ATGATACCAGGAAAGACGGT+CGG | 0.635685 | 5:-73664228 | MsG0580028042.01.T01:CDS |
| ATGTCTGTAATATAGAAGCT+GGG | 0.666658 | 5:-73664170 | MsG0580028042.01.T01:CDS |
| GAAGTAGGAGTATTATAACC+AGG | 0.672487 | 5:+73664135 | None:intergenic |
| AAGATAACATGACCACACAA+TGG | 0.675526 | 5:-73664335 | MsG0580028042.01.T01:CDS |
| AGTTCATGATAAAGATACGC+CGG | 0.676724 | 5:-73664274 | MsG0580028042.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAATATTTTGTTCTCTTC+TGG | + | Chr5:73664092-73664111 | None:intergenic | 25.0% |
| ATGTCTGTAATATAGAAGCT+GGG | - | Chr5:73664228-73664247 | MsG0580028042.01.T01:CDS | 30.0% | |
| TATGATGAATTTAGAGGAAG+TGG | - | Chr5:73664320-73664339 | MsG0580028042.01.T01:CDS | 30.0% | |
| TCTGGTTATGATGAATTTAG+AGG | - | Chr5:73664314-73664333 | MsG0580028042.01.T01:CDS | 30.0% | |
| TGATGTGATGAGACAATATA+AGG | - | Chr5:73664193-73664212 | MsG0580028042.01.T01:CDS | 30.0% | |
| ! | TTCACCTTTTACCTTAGATT+GGG | - | Chr5:73664283-73664302 | MsG0580028042.01.T01:CDS | 30.0% |
| AAAGTAGCTGAAATGATACC+AGG | - | Chr5:73664158-73664177 | MsG0580028042.01.T01:CDS | 35.0% | |
| AAGATAACATGACCACACAA+TGG | - | Chr5:73664063-73664082 | MsG0580028042.01.T01:CDS | 35.0% | |
| ACAAAACTCCAATTGGTGTA+TGG | - | Chr5:73664040-73664059 | MsG0580028042.01.T01:CDS | 35.0% | |
| AGTTCATGATAAAGATACGC+CGG | - | Chr5:73664124-73664143 | MsG0580028042.01.T01:CDS | 35.0% | |
| CATACACACAAAACTCCAAT+TGG | - | Chr5:73664033-73664052 | MsG0580028042.01.T01:CDS | 35.0% | |
| GATGTCTGTAATATAGAAGC+TGG | - | Chr5:73664227-73664246 | MsG0580028042.01.T01:CDS | 35.0% | |
| GTTATCTTCCATACACCAAT+TGG | + | Chr5:73664051-73664070 | None:intergenic | 35.0% | |
| TGAAATGATACCAGGAAAGA+CGG | - | Chr5:73664166-73664185 | MsG0580028042.01.T01:CDS | 35.0% | |
| ! | CTTCACCTTTTACCTTAGAT+TGG | - | Chr5:73664282-73664301 | MsG0580028042.01.T01:CDS | 35.0% |
| ! | TCTAAGGTAAAAGGTGAAGT+AGG | + | Chr5:73664281-73664300 | None:intergenic | 35.0% |
| !! | GAAGTAGGAGTATTATAACC+AGG | + | Chr5:73664266-73664285 | None:intergenic | 35.0% |
| TTGTGTTCTTCTTCAGTCCA+TGG | + | Chr5:73664398-73664417 | None:intergenic | 40.0% | |
| TTTGTGTGTATGATGCAGGA+GGG | + | Chr5:73664025-73664044 | None:intergenic | 40.0% | |
| ! | CTCACCCAATCTAAGGTAAA+AGG | + | Chr5:73664290-73664309 | None:intergenic | 40.0% |
| ! | GGAGTATTATAACCAGGAAC+TGG | + | Chr5:73664260-73664279 | None:intergenic | 40.0% |
| ! | TTTTGTGTGTATGATGCAGG+AGG | + | Chr5:73664026-73664045 | None:intergenic | 40.0% |
| !! | GAGTTTTGTGTGTATGATGC+AGG | + | Chr5:73664029-73664048 | None:intergenic | 40.0% |
| AGAACTGCTCACCCAATCTA+AGG | + | Chr5:73664297-73664316 | None:intergenic | 45.0% | |
| ATAAAGATACGCCGGATCGA+TGG | - | Chr5:73664132-73664151 | MsG0580028042.01.T01:CDS | 45.0% | |
| ATGATACCAGGAAAGACGGT+CGG | - | Chr5:73664170-73664189 | MsG0580028042.01.T01:CDS | 45.0% | |
| TTAGATTGGGTGAGCAGTTC+TGG | - | Chr5:73664296-73664315 | MsG0580028042.01.T01:CDS | 45.0% | |
| TTTAGTTAGAGCTCCTGAGC+AGG | - | Chr5:73664355-73664374 | MsG0580028042.01.T01:CDS | 45.0% | |
| ! | GCTACTTTATGCCATCGATC+CGG | + | Chr5:73664146-73664165 | None:intergenic | 45.0% |
| !! | AAAGGAAGAAAGGAGTGCCA+TGG | - | Chr5:73664378-73664397 | MsG0580028042.01.T01:CDS | 45.0% |
| CCTGAGCAGGAAAGGAAGAA+AGG | - | Chr5:73664368-73664387 | MsG0580028042.01.T01:CDS | 50.0% | |
| CCTTTCTTCCTTTCCTGCTC+AGG | + | Chr5:73664371-73664390 | None:intergenic | 50.0% | |
| TTAGAGCTCCTGAGCAGGAA+AGG | - | Chr5:73664360-73664379 | MsG0580028042.01.T01:CDS | 50.0% | |
| !! | CTCTTCTGGAGTCCATTGTG+TGG | + | Chr5:73664078-73664097 | None:intergenic | 50.0% |
| ACATCACCGACCGTCTTTCC+TGG | + | Chr5:73664179-73664198 | None:intergenic | 55.0% | |
| !! | GCTGGGTTGATTCCAGTTCC+TGG | - | Chr5:73664245-73664264 | MsG0580028042.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 73663999 | 73664421 | 73663999 | ID=MsG0580028042.01;Name=MsG0580028042.01 |
| Chr5 | mRNA | 73663999 | 73664421 | 73663999 | ID=MsG0580028042.01.T01;Parent=MsG0580028042.01;Name=MsG0580028042.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|140 |
| Chr5 | exon | 73663999 | 73664421 | 73663999 | ID=MsG0580028042.01.T01:exon:45478;Parent=MsG0580028042.01.T01 |
| Chr5 | CDS | 73663999 | 73664421 | 73663999 | ID=MsG0580028042.01.T01:cds;Parent=MsG0580028042.01.T01 |
| Gene Sequence |
| Protein sequence |
