Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028269.01.T01 | KEH28124.1 | 95.041 | 121 | 6 | 0 | 1 | 121 | 1 | 121 | 2.68E-79 | 243 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028269.01.T01 | Q8GXL7 | 40.708 | 113 | 42 | 5 | 34 | 122 | 18 | 129 | 1.24E-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028269.01.T01 | A0A072UEB1 | 95.041 | 121 | 6 | 0 | 1 | 121 | 1 | 121 | 1.28e-79 | 243 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580028269.01.T01 | TF | Tify |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028269.01.T01 | MTR_5g066510 | 95.041 | 121 | 6 | 0 | 1 | 121 | 1 | 121 | 3.25e-83 | 243 |
| MsG0580028269.01.T01 | MTR_5g066510 | 95.041 | 121 | 6 | 0 | 1 | 121 | 1 | 121 | 1.01e-81 | 243 |
| MsG0580028269.01.T01 | MTR_1g061610 | 67.647 | 68 | 21 | 1 | 39 | 106 | 42 | 108 | 9.17e-26 | 95.1 |
| MsG0580028269.01.T01 | MTR_3g100050 | 67.213 | 61 | 19 | 1 | 46 | 105 | 42 | 102 | 2.23e-19 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028269.01.T01 | AT3G21175 | 43.750 | 96 | 31 | 4 | 34 | 107 | 18 | 112 | 1.01e-12 | 63.5 |
| MsG0580028269.01.T01 | AT3G21175 | 40.708 | 113 | 42 | 5 | 34 | 122 | 18 | 129 | 1.26e-12 | 63.9 |
| MsG0580028269.01.T01 | AT1G51600 | 40.517 | 116 | 40 | 4 | 34 | 121 | 18 | 132 | 2.50e-12 | 63.2 |
| MsG0580028269.01.T01 | AT1G51600 | 40.517 | 116 | 40 | 4 | 34 | 121 | 18 | 132 | 2.50e-12 | 63.2 |
| MsG0580028269.01.T01 | AT3G21175 | 43.750 | 96 | 31 | 4 | 34 | 107 | 18 | 112 | 2.52e-12 | 63.2 |
Find 52 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAACCTCTACAACAAATT+AGG | 0.141320 | 5:+77553292 | MsG0580028269.01.T01:five_prime_UTR |
| CGCTTCTCAAATTCTCTCTT+TGG | 0.239601 | 5:+77553323 | MsG0580028269.01.T01:five_prime_UTR |
| AAACTTTGAAGTGCAAATTC+AGG | 0.278085 | 5:-77553533 | None:intergenic |
| TCCAACTCCGATGCTGTTTA+CGG | 0.309806 | 5:+77553494 | MsG0580028269.01.T01:CDS |
| AAAACCTCTACAACAAATTA+GGG | 0.317019 | 5:+77553293 | MsG0580028269.01.T01:five_prime_UTR |
| TCGCTTTCGCCGGCGGTGAT+TGG | 0.347965 | 5:-77553398 | None:intergenic |
| GGCGAAAGCGACGACGGTTC+CGG | 0.410307 | 5:+77553410 | MsG0580028269.01.T01:CDS |
| GTCGATGCCGGCGTCGGGAC+CGG | 0.418465 | 5:-77553429 | None:intergenic |
| CAGCTTACGCTGTCGTTTCG+TGG | 0.436824 | 5:+77553569 | MsG0580028269.01.T01:CDS |
| CGTACTGAATTTGATGTTGA+GGG | 0.442697 | 5:-77553451 | None:intergenic |
| TCAATCGGTGTTGTTACTGT+TGG | 0.455881 | 5:+77553850 | MsG0580028269.01.T01:CDS |
| CAATCGGTGTTGTTACTGTT+GGG | 0.455920 | 5:+77553851 | MsG0580028269.01.T01:CDS |
| TGAGGGTCGATGCCGGCGTC+GGG | 0.466145 | 5:-77553434 | None:intergenic |
| AAATTCTCTCTTTGGTGCAA+TGG | 0.471067 | 5:+77553331 | MsG0580028269.01.T01:exon |
| CGGTGCCTCAACAAAGTTTG+AGG | 0.475750 | 5:+77553906 | MsG0580028269.01.T01:CDS |
| ATTGAGAATCGAACCTTAGC+AGG | 0.477071 | 5:-77553620 | None:intergenic |
| GCCGTAAACAGCATCGGAGT+TGG | 0.479472 | 5:-77553495 | None:intergenic |
| TCAGTACGAAACGCACGGTA+TGG | 0.494591 | 5:+77553466 | MsG0580028269.01.T01:CDS |
| TGACTCTGTTACGCCTGCTA+AGG | 0.495608 | 5:+77553607 | MsG0580028269.01.T01:CDS |
| ACCGCATTCACACCAGCATC+AGG | 0.496179 | 5:-77553887 | None:intergenic |
| TTTCATGTTACAGGTTCAAT+CGG | 0.502821 | 5:+77553835 | MsG0580028269.01.T01:intron |
| GGAACCGTCGTCGCTTTCGC+CGG | 0.503198 | 5:-77553408 | None:intergenic |
| TTGAGGGTCGATGCCGGCGT+CGG | 0.505787 | 5:-77553435 | None:intergenic |
| GACGGTTCCGGTCCCGACGC+CGG | 0.509123 | 5:+77553422 | MsG0580028269.01.T01:CDS |
| TCCGATGCTGTTTACGGCAA+CGG | 0.517634 | 5:+77553500 | MsG0580028269.01.T01:CDS |
| TCGTACTGAATTTGATGTTG+AGG | 0.518430 | 5:-77553452 | None:intergenic |
| ATTGGTACTGTGGTGTTCAA+AGG | 0.519474 | 5:-77553380 | None:intergenic |
| GAAACCCTAATTTGTTGTAG+AGG | 0.536285 | 5:-77553297 | None:intergenic |
| GGTTGAGAGTGTTCGTACAT+TGG | 0.539771 | 5:-77553359 | None:intergenic |
| ACCGTCGTCGCTTTCGCCGG+CGG | 0.540258 | 5:-77553405 | None:intergenic |
| TGAGAGTGTTCGTACATTGG+TGG | 0.550890 | 5:-77553356 | None:intergenic |
| GTGATTAATTTGCCGATCTG+TGG | 0.563895 | 5:+77554068 | MsG0580028269.01.T01:CDS |
| TTGATGTTGAGGGTCGATGC+CGG | 0.564041 | 5:-77553441 | None:intergenic |
| GGCTTGCTAAATGCTAGCTT+AGG | 0.567617 | 5:+77554089 | MsG0580028269.01.T01:CDS |
| GGATGTGAACAACCTGATGC+TGG | 0.568670 | 5:+77553875 | MsG0580028269.01.T01:CDS |
| GAAGAGAGTGCAGAAGGTGT+AGG | 0.575675 | 5:-77553231 | None:intergenic |
| GGTGCCTCAACAAAGTTTGA+GGG | 0.580121 | 5:+77553907 | MsG0580028269.01.T01:CDS |
| GCCGTTGCCGTAAACAGCAT+CGG | 0.590158 | 5:-77553501 | None:intergenic |
| GCATTTAGCAAGCCACAGAT+CGG | 0.600509 | 5:-77554080 | None:intergenic |
| TCGGTGTTGTTACTGTTGGG+TGG | 0.618046 | 5:+77553854 | MsG0580028269.01.T01:CDS |
| TTTGTGTTTGCGAAAGCGAG+GGG | 0.626213 | 5:-77553254 | None:intergenic |
| ACCACCCTCAAACTTTGTTG+AGG | 0.626395 | 5:-77553911 | None:intergenic |
| GGGAAGTAAATACTAGTGAG+TGG | 0.631263 | 5:+77554036 | MsG0580028269.01.T01:CDS |
| CTTAGGTCAACAATACCTGT+AGG | 0.631818 | 5:+77554106 | MsG0580028269.01.T01:CDS |
| ACCGCCGGCGAAAGCGACGA+CGG | 0.634534 | 5:+77553404 | MsG0580028269.01.T01:CDS |
| ACCACAGTACCAATCACCGC+CGG | 0.655305 | 5:+77553389 | MsG0580028269.01.T01:CDS |
| GCCTCAACAAAGTTTGAGGG+TGG | 0.655336 | 5:+77553910 | MsG0580028269.01.T01:CDS |
| GATGCTGTTTACGGCAACGG+CGG | 0.661829 | 5:+77553503 | MsG0580028269.01.T01:CDS |
| ACCTGATGCTGGTGTGAATG+CGG | 0.662406 | 5:+77553886 | MsG0580028269.01.T01:CDS |
| GCCGGCGGTGATTGGTACTG+TGG | 0.669157 | 5:-77553390 | None:intergenic |
| CAAATTCAGTACGAAACGCA+CGG | 0.681403 | 5:+77553461 | MsG0580028269.01.T01:CDS |
| TTGTGTTTGCGAAAGCGAGG+GGG | 0.698518 | 5:-77553253 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTGAATTTTTTAGTTATT+TGG | + | Chr5:77553792-77553811 | MsG0580028269.01.T01:intron | 15.0% |
| !!! | GTTGAATTTTTTAGTTATTT+GGG | + | Chr5:77553793-77553812 | MsG0580028269.01.T01:intron | 15.0% |
| !!! | TGTTTATTATTGATTTTTCT+TGG | + | Chr5:77553673-77553692 | MsG0580028269.01.T01:intron | 15.0% |
| !! | ATTTGAATATGCATTAGATT+TGG | + | Chr5:77554015-77554034 | MsG0580028269.01.T01:intron | 20.0% |
| !! | TTTGAATATGCATTAGATTT+GGG | + | Chr5:77554016-77554035 | MsG0580028269.01.T01:intron | 20.0% |
| ! | AAAACCTCTACAACAAATTA+GGG | + | Chr5:77553293-77553312 | MsG0580028269.01.T01:five_prime_UTR | 25.0% |
| !!! | TAGAGGTTTTGAGTTTAATT+GGG | - | Chr5:77553283-77553302 | None:intergenic | 25.0% |
| CAAAACCTCTACAACAAATT+AGG | + | Chr5:77553292-77553311 | MsG0580028269.01.T01:five_prime_UTR | 30.0% | |
| TGGGATTGAAAATTCGTATT+TGG | + | Chr5:77553812-77553831 | MsG0580028269.01.T01:intron | 30.0% | |
| TTTCATGTTACAGGTTCAAT+CGG | + | Chr5:77553835-77553854 | MsG0580028269.01.T01:intron | 30.0% | |
| ! | ATTTTCAACATGTTACAAGC+TGG | - | Chr5:77553990-77554009 | None:intergenic | 30.0% |
| !! | AAACTTTGAAGTGCAAATTC+AGG | - | Chr5:77553536-77553555 | None:intergenic | 30.0% |
| !!! | GTAGAGGTTTTGAGTTTAAT+TGG | - | Chr5:77553284-77553303 | None:intergenic | 30.0% |
| AAGGTTCGATTCTCAATTGT+TGG | + | Chr5:77553626-77553645 | MsG0580028269.01.T01:intron | 35.0% | |
| CGTACTGAATTTGATGTTGA+GGG | - | Chr5:77553454-77553473 | None:intergenic | 35.0% | |
| TCGTACTGAATTTGATGTTG+AGG | - | Chr5:77553455-77553474 | None:intergenic | 35.0% | |
| ! | AAATTCTCTCTTTGGTGCAA+TGG | + | Chr5:77553331-77553350 | MsG0580028269.01.T01:exon | 35.0% |
| ! | CGTATTTGGTTTCATGTTAC+AGG | + | Chr5:77553826-77553845 | MsG0580028269.01.T01:intron | 35.0% |
| !! | GAAACCCTAATTTGTTGTAG+AGG | - | Chr5:77553300-77553319 | None:intergenic | 35.0% |
| ATTGAGAATCGAACCTTAGC+AGG | - | Chr5:77553623-77553642 | None:intergenic | 40.0% | |
| ATTGGTACTGTGGTGTTCAA+AGG | - | Chr5:77553383-77553402 | None:intergenic | 40.0% | |
| CAAATTCAGTACGAAACGCA+CGG | + | Chr5:77553461-77553480 | MsG0580028269.01.T01:CDS | 40.0% | |
| CGCTTCTCAAATTCTCTCTT+TGG | + | Chr5:77553323-77553342 | MsG0580028269.01.T01:five_prime_UTR | 40.0% | |
| CTTAGGTCAACAATACCTGT+AGG | + | Chr5:77554106-77554125 | MsG0580028269.01.T01:CDS | 40.0% | |
| GGGAAGTAAATACTAGTGAG+TGG | + | Chr5:77554036-77554055 | MsG0580028269.01.T01:CDS | 40.0% | |
| ! | TTTTGTGTTTGCGAAAGCGA+GGG | - | Chr5:77553258-77553277 | None:intergenic | 40.0% |
| ! | TTTTTGTGTTTGCGAAAGCG+AGG | - | Chr5:77553259-77553278 | None:intergenic | 40.0% |
| !! | CAATCGGTGTTGTTACTGTT+GGG | + | Chr5:77553851-77553870 | MsG0580028269.01.T01:CDS | 40.0% |
| !! | GTGATTAATTTGCCGATCTG+TGG | + | Chr5:77554068-77554087 | MsG0580028269.01.T01:CDS | 40.0% |
| !! | TCAATCGGTGTTGTTACTGT+TGG | + | Chr5:77553850-77553869 | MsG0580028269.01.T01:CDS | 40.0% |
| ACCACCCTCAAACTTTGTTG+AGG | - | Chr5:77553914-77553933 | None:intergenic | 45.0% | |
| GCATTTAGCAAGCCACAGAT+CGG | - | Chr5:77554083-77554102 | None:intergenic | 45.0% | |
| GGTGCCTCAACAAAGTTTGA+GGG | + | Chr5:77553907-77553926 | MsG0580028269.01.T01:CDS | 45.0% | |
| TTTGTGTTTGCGAAAGCGAG+GGG | - | Chr5:77553257-77553276 | None:intergenic | 45.0% | |
| ! | TCCAACTCCGATGCTGTTTA+CGG | + | Chr5:77553494-77553513 | MsG0580028269.01.T01:CDS | 45.0% |
| !! | GGCTTGCTAAATGCTAGCTT+AGG | + | Chr5:77554089-77554108 | MsG0580028269.01.T01:CDS | 45.0% |
| !! | GGTTGAGAGTGTTCGTACAT+TGG | - | Chr5:77553362-77553381 | None:intergenic | 45.0% |
| !! | TGAGAGTGTTCGTACATTGG+TGG | - | Chr5:77553359-77553378 | None:intergenic | 45.0% |
| ACCTGATGCTGGTGTGAATG+CGG | + | Chr5:77553886-77553905 | MsG0580028269.01.T01:CDS | 50.0% | |
| CGGTGCCTCAACAAAGTTTG+AGG | + | Chr5:77553906-77553925 | MsG0580028269.01.T01:CDS | 50.0% | |
| GCCTCAACAAAGTTTGAGGG+TGG | + | Chr5:77553910-77553929 | MsG0580028269.01.T01:CDS | 50.0% | |
| GGATGTGAACAACCTGATGC+TGG | + | Chr5:77553875-77553894 | MsG0580028269.01.T01:CDS | 50.0% | |
| TCAGTACGAAACGCACGGTA+TGG | + | Chr5:77553466-77553485 | MsG0580028269.01.T01:CDS | 50.0% | |
| TGACTCTGTTACGCCTGCTA+AGG | + | Chr5:77553607-77553626 | MsG0580028269.01.T01:CDS | 50.0% | |
| TTGATGTTGAGGGTCGATGC+CGG | - | Chr5:77553444-77553463 | None:intergenic | 50.0% | |
| TTGTGTTTGCGAAAGCGAGG+GGG | - | Chr5:77553256-77553275 | None:intergenic | 50.0% | |
| ! | GAAGAGAGTGCAGAAGGTGT+AGG | - | Chr5:77553234-77553253 | None:intergenic | 50.0% |
| ! | TCCGATGCTGTTTACGGCAA+CGG | + | Chr5:77553500-77553519 | MsG0580028269.01.T01:CDS | 50.0% |
| !! | TCGGTGTTGTTACTGTTGGG+TGG | + | Chr5:77553854-77553873 | MsG0580028269.01.T01:CDS | 50.0% |
| ACCACAGTACCAATCACCGC+CGG | + | Chr5:77553389-77553408 | MsG0580028269.01.T01:CDS | 55.0% | |
| ACCGCATTCACACCAGCATC+AGG | - | Chr5:77553890-77553909 | None:intergenic | 55.0% | |
| GCCGTTGCCGTAAACAGCAT+CGG | - | Chr5:77553504-77553523 | None:intergenic | 55.0% | |
| ! | CAGCTTACGCTGTCGTTTCG+TGG | + | Chr5:77553569-77553588 | MsG0580028269.01.T01:CDS | 55.0% |
| ! | GATGCTGTTTACGGCAACGG+CGG | + | Chr5:77553503-77553522 | MsG0580028269.01.T01:CDS | 55.0% |
| ! | GCCGTAAACAGCATCGGAGT+TGG | - | Chr5:77553498-77553517 | None:intergenic | 55.0% |
| ! | GAGGGGGAAGAGAGTGCAGA+AGG | - | Chr5:77553240-77553259 | None:intergenic | 60.0% |
| GCCGGCGGTGATTGGTACTG+TGG | - | Chr5:77553393-77553412 | None:intergenic | 65.0% | |
| GGCGAAAGCGACGACGGTTC+CGG | + | Chr5:77553410-77553429 | MsG0580028269.01.T01:CDS | 65.0% | |
| TCGCTTTCGCCGGCGGTGAT+TGG | - | Chr5:77553401-77553420 | None:intergenic | 65.0% | |
| TTGAGGGTCGATGCCGGCGT+CGG | - | Chr5:77553438-77553457 | None:intergenic | 65.0% | |
| ! | GGAACCGTCGTCGCTTTCGC+CGG | - | Chr5:77553411-77553430 | None:intergenic | 65.0% |
| ACCGCCGGCGAAAGCGACGA+CGG | + | Chr5:77553404-77553423 | MsG0580028269.01.T01:CDS | 70.0% | |
| TGAGGGTCGATGCCGGCGTC+GGG | - | Chr5:77553437-77553456 | None:intergenic | 70.0% | |
| ! | ACCGTCGTCGCTTTCGCCGG+CGG | - | Chr5:77553408-77553427 | None:intergenic | 70.0% |
| GACGGTTCCGGTCCCGACGC+CGG | + | Chr5:77553422-77553441 | MsG0580028269.01.T01:CDS | 75.0% | |
| ! | GTCGATGCCGGCGTCGGGAC+CGG | - | Chr5:77553432-77553451 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 77553230 | 77554127 | 77553230 | ID=MsG0580028269.01;Name=MsG0580028269.01 |
| Chr5 | mRNA | 77553230 | 77554127 | 77553230 | ID=MsG0580028269.01.T01;Parent=MsG0580028269.01;Name=MsG0580028269.01.T01;_AED=0.48;_eAED=0.56;_QI=120|0.5|0.66|1|1|1|3|0|152 |
| Chr5 | exon | 77553230 | 77553628 | 77553230 | ID=MsG0580028269.01.T01:exon:24179;Parent=MsG0580028269.01.T01 |
| Chr5 | exon | 77553848 | 77553931 | 77553848 | ID=MsG0580028269.01.T01:exon:24180;Parent=MsG0580028269.01.T01 |
| Chr5 | exon | 77554032 | 77554127 | 77554032 | ID=MsG0580028269.01.T01:exon:24181;Parent=MsG0580028269.01.T01 |
| Chr5 | five_prime_UTR | 77553230 | 77553349 | 77553230 | ID=MsG0580028269.01.T01:five_prime_utr;Parent=MsG0580028269.01.T01 |
| Chr5 | CDS | 77553350 | 77553628 | 77553350 | ID=MsG0580028269.01.T01:cds;Parent=MsG0580028269.01.T01 |
| Chr5 | CDS | 77553848 | 77553931 | 77553848 | ID=MsG0580028269.01.T01:cds;Parent=MsG0580028269.01.T01 |
| Chr5 | CDS | 77554032 | 77554127 | 77554032 | ID=MsG0580028269.01.T01:cds;Parent=MsG0580028269.01.T01 |
| Gene Sequence |
| Protein sequence |
