AlfalfaGEDB | MsG0580028352.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028352.01

KEGG: -
GO: GO:0005515 GO:0005622 GO:0008270
PFAM: PF06203

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028352.01.T01	XP_003615555.1	95.638	298	13	0	1	298	1	298	0	586
MsG0580028352.01.T01	XP_003615555.1	97.368	38	1	0	311	348	373	410	7.78E-13	81.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028352.01.T01	Q9SSE5	45.209	407	127	11	1	347	1	371	9.70E-99	299

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028352.01.T01	G7KE22	95.638	298	13	0	1	298	1	298	0.0	586

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TFs/TRs:

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Gene ID	Type	Classification
MsG0580028352.01.T01	TF	C2C2-CO-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028352.01.T01	MTR_5g069480	95.638	298	13	0	1	298	1	298	0.0	586
MsG0580028352.01.T01	MTR_5g069480	97.368	38	1	0	311	348	373	410	9.42e-17	81.3
MsG0580028352.01.T01	MTR_5g069480	96.269	268	10	0	1	268	1	268	0.0	531
MsG0580028352.01.T01	MTR_3g082630	54.458	415	116	10	1	347	1	410	4.60e-130	378
MsG0580028352.01.T01	MTR_2g088900	33.121	314	170	9	1	296	97	388	4.73e-39	145
MsG0580028352.01.T01	MTR_2g088900	83.333	36	6	0	307	342	482	517	9.32e-13	69.3
MsG0580028352.01.T01	MTR_1g109350	49.138	116	55	2	1	115	1	113	4.02e-30	118
MsG0580028352.01.T01	MTR_7g108150	49.505	101	50	1	5	105	20	119	3.26e-29	116
MsG0580028352.01.T01	MTR_1g013450	27.410	332	198	10	4	332	5	296	2.18e-25	104
MsG0580028352.01.T01	MTR_7g083540	40.909	110	59	1	1	104	1	110	2.38e-21	94.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028352.01.T01	AT3G07650	45.209	407	127	11	1	347	1	371	9.89e-100	299
MsG0580028352.01.T01	AT3G07650	45.209	407	127	11	1	347	1	371	9.89e-100	299
MsG0580028352.01.T01	AT3G07650	45.209	407	127	11	1	347	1	371	9.89e-100	299
MsG0580028352.01.T01	AT3G07650	45.209	407	127	11	1	347	1	371	9.89e-100	299
MsG0580028352.01.T01	AT5G48250	45.098	408	127	12	1	347	1	372	1.17e-96	291
MsG0580028352.01.T01	AT4G15250	54.717	106	46	1	1	106	1	104	1.54e-35	132
MsG0580028352.01.T01	AT4G15250	77.143	35	8	0	307	341	292	326	1.02e-11	65.5
MsG0580028352.01.T01	AT2G33500	49.020	102	52	0	5	106	12	113	2.06e-33	128
MsG0580028352.01.T01	AT2G33500	49.020	102	52	0	5	106	12	113	2.22e-33	128
MsG0580028352.01.T01	AT3G21880	50.000	116	56	1	1	116	5	118	4.66e-33	126

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Find 104 sgRNAs with CRISPR-Local

Find 394 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACATGTCTGCTAGTGC+TGG	0.564402	5:-78937677	MsG0580028352.01.T01:CDS
AAAGGCCGGTTCGTCAAAGC+TGG	0.416312	5:-78933864	MsG0580028352.01.T01:CDS
AAAGTCATATTTCAATGATT+TGG	0.289645	5:-78933639	MsG0580028352.01.T01:three_prime_UTR
AACTCCATCTACATGTATGC+AGG	0.534293	5:+78933328	None:intergenic
AACTGTGATTGGTCAGCTCA+TGG	0.551188	5:-78939216	MsG0580028352.01.T01:CDS
AAGGCCGGTTCGTCAAAGCT+GGG	0.589643	5:-78933863	MsG0580028352.01.T01:CDS
AAGGGAACGCGCATCGGTGA+AGG	0.559094	5:-78933602	MsG0580028352.01.T01:three_prime_UTR
ACAATTCATTGTCAGTCCTT+TGG	0.393417	5:-78933465	MsG0580028352.01.T01:three_prime_UTR
ACGGGGTTTGGCACTTGACT+CGG	0.451656	5:+78938921	None:intergenic
AGAACAGTGACAACAGTGCC+AGG	0.462494	5:-78939035	MsG0580028352.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATTCTTCGTTTCTGCA+AGG	+	Chr5:78936577-78936596	None:intergenic	30.0%
AAACTTGAACTAATTGCTAC+TGG	-	Chr5:78935088-78935107	MsG0580028352.01.T01:intron	30.0%
AAATCATGAAGCTATACCTT+AGG	+	Chr5:78933713-78933732	None:intergenic	30.0%
AACATTAAGTTTCCTTTGGT+AGG	-	Chr5:78935583-78935602	MsG0580028352.01.T01:intron	30.0%
AATTTATTGAACCAGTGACT+CGG	+	Chr5:78937188-78937207	None:intergenic	30.0%
AGAAATACAAAACCTACCAA+AGG	+	Chr5:78935598-78935617	None:intergenic	30.0%
AGAGAACATTAAGTTTCCTT+TGG	-	Chr5:78935579-78935598	MsG0580028352.01.T01:intron	30.0%
AGAGGGATAGAATATTGTAT+TGG	-	Chr5:78938230-78938249	MsG0580028352.01.T01:intron	30.0%
AGCAGATTGAATAGAATTCT+CGG	+	Chr5:78937429-78937448	None:intergenic	30.0%
ATACAGACACGAAACAATAA+AGG	+	Chr5:78934051-78934070	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	78933146	78939445	78933146	ID=MsG0580028352.01;Name=MsG0580028352.01
Chr5	mRNA	78933146	78939445	78933146	ID=MsG0580028352.01.T01;Parent=MsG0580028352.01;Name=MsG0580028352.01.T01;_AED=0.39;_eAED=0.40;_QI=0\|0\|0.25\|1\|1\|1\|4\|672\|348
Chr5	exon	78933146	78933947	78933146	ID=MsG0580028352.01.T01:exon:15818;Parent=MsG0580028352.01.T01
Chr5	exon	78936819	78936931	78936819	ID=MsG0580028352.01.T01:exon:15817;Parent=MsG0580028352.01.T01
Chr5	exon	78937643	78937876	78937643	ID=MsG0580028352.01.T01:exon:15816;Parent=MsG0580028352.01.T01
Chr5	exon	78938876	78939445	78938876	ID=MsG0580028352.01.T01:exon:15815;Parent=MsG0580028352.01.T01
Chr5	CDS	78938876	78939445	78938876	ID=MsG0580028352.01.T01:cds;Parent=MsG0580028352.01.T01
Chr5	CDS	78937643	78937876	78937643	ID=MsG0580028352.01.T01:cds;Parent=MsG0580028352.01.T01
Chr5	CDS	78936819	78936931	78936819	ID=MsG0580028352.01.T01:cds;Parent=MsG0580028352.01.T01
Chr5	CDS	78933818	78933947	78933818	ID=MsG0580028352.01.T01:cds;Parent=MsG0580028352.01.T01
Chr5	three_prime_UTR	78933146	78933817	78933146	ID=MsG0580028352.01.T01:three_prime_utr;Parent=MsG0580028352.01.T01

Gene Sequence

>MsG0580028352.01.T01

ATGGGTTCTTTATGCGATTTTTGCGGAGACCAGAGGTCTTTGGTGTACTGCCGCTCTGATGCTGCTTCCTTATGTTTGTCGTGTGATCGAAATGTTCATTCTGCTAACGAACTTTCTAAGCGTCATTCAAGAACACTTGTATGCGAGAGGTGTAATTTACAACCTGCATATGTGAGGTGTGTTGAAGAGAAAGTTTCACTTTGTCAGAACTGTGATTGGTCAGCTCATGGTACCAATCCATCCTCTTCAACACACAAGAGGCAATCAATCAATTGCTTCTCTGGCTGTCCTTCGGCTTCCGAGCTTTCTTCAATATGGCCCTTTTTCTCAGACATCCCTTCTACGGGTGAAGCATGTGAACATAAACTCGGTTTGATGAGCATTAACGAGAACAGTGACAACAGTGCCAGGGTTCCTCCAGAAAGCAAGAGCGTGTCTGGTTCAGCTCAAGTGGCCGATTTTCCAAGCAAAAACAAATCTGGCGTCGACACATCTTCAATACCCGAGTCAAGTGCCAAACCCCGTATTTTGAACCAGGCGCCCGGATCTTCCAATGAACTTATGCCTAAGTTACTATGTCCCAGCATAAAGGCTCCTGCACTATGTGAAGATGATAAGTTGCTTGGTGATTTCAACATAGATGAGATGGATTTTGAACTTGAGAACTATACGGAACTTTTTGACTTTGCTCTCAACTACTCTGAAGAGTTTTTTGAAAATGGTGGTATTGATAGCTTATTTGAGAGAAAAGACATGTCTGCTAGTGCTGGTGATTCAAATTGTCAAGGCGCTGTTGCTGCTGAGGGGTCATCAGCAAGGTTTGTTAGTGCTATCCAACCAGAATGCAGCAATGCTGCATCTGCAGATTCAATATTAAGTACCAGGCTCGGCCCTTGGCATGTGCAAGGTGAGCTACGGTTTGATAAGAAAGTAAGATATGCTTCTCAGAAGGCGAGGGCTGATGTCAGAAAACGCGTGAAAGGCCGGTTCGTCAAAGCTGGGGAAACATACGATTACGACCCTTTGAGTCAAACCAGAAGCTGCTGA

Protein sequence

>MsG0580028352.01.T01

MGSLCDFCGDQRSLVYCRSDAASLCLSCDRNVHSANELSKRHSRTLVCERCNLQPAYVRCVEEKVSLCQNCDWSAHGTNPSSSTHKRQSINCFSGCPSASELSSIWPFFSDIPSTGEACEHKLGLMSINENSDNSARVPPESKSVSGSAQVADFPSKNKSGVDTSSIPESSAKPRILNQAPGSSNELMPKLLCPSIKAPALCEDDKLLGDFNIDEMDFELENYTELFDFALNYSEEFFENGGIDSLFERKDMSASAGDSNCQGAVAAEGSSARFVSAIQPECSNAASADSILSTRLGPWHVQGELRFDKKVRYASQKARADVRKRVKGRFVKAGETYDYDPLSQTRSC*