Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028994.01.T01 | XP_024640742.1 | 94.22 | 173 | 4 | 2 | 1 | 173 | 1 | 167 | 3.61E-111 | 324 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028994.01.T01 | Q3E9B4 | 53.03 | 66 | 31 | 0 | 97 | 162 | 154 | 219 | 8.46E-20 | 85.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028994.01.T01 | A0A396HWM6 | 94.220 | 173 | 4 | 2 | 1 | 173 | 1 | 167 | 1.72e-111 | 324 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580028994.01.T01 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028994.01.T01 | MTR_5g079710 | 94.152 | 171 | 4 | 2 | 1 | 171 | 1 | 165 | 8.01e-113 | 322 |
| MsG0580028994.01.T01 | MTR_4g052040 | 47.619 | 168 | 78 | 4 | 6 | 166 | 4 | 168 | 9.71e-40 | 133 |
| MsG0580028994.01.T01 | MTR_4g052060 | 41.071 | 168 | 92 | 4 | 3 | 167 | 1 | 164 | 1.35e-32 | 115 |
| MsG0580028994.01.T01 | MTR_7g105790 | 37.714 | 175 | 86 | 5 | 6 | 161 | 4 | 174 | 2.31e-24 | 94.7 |
| MsG0580028994.01.T01 | MTR_0907s0020 | 59.420 | 69 | 28 | 0 | 97 | 165 | 102 | 170 | 1.18e-23 | 92.4 |
| MsG0580028994.01.T01 | MTR_8g103520 | 40.230 | 87 | 52 | 0 | 80 | 166 | 207 | 293 | 3.72e-18 | 80.1 |
| MsG0580028994.01.T01 | MTR_3g111780 | 50.725 | 69 | 34 | 0 | 94 | 162 | 195 | 263 | 1.36e-12 | 64.7 |
| MsG0580028994.01.T01 | MTR_5g075210 | 43.284 | 67 | 38 | 0 | 88 | 154 | 271 | 337 | 1.89e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028994.01.T01 | AT3G52525 | 60.317 | 63 | 25 | 0 | 98 | 160 | 67 | 129 | 3.72e-21 | 85.5 |
| MsG0580028994.01.T01 | AT5G19650 | 53.030 | 66 | 31 | 0 | 97 | 162 | 154 | 219 | 8.63e-21 | 85.9 |
| MsG0580028994.01.T01 | AT2G36026 | 60.656 | 61 | 24 | 0 | 102 | 162 | 91 | 151 | 6.79e-20 | 82.8 |
| MsG0580028994.01.T01 | AT2G18500 | 48.387 | 62 | 32 | 0 | 95 | 156 | 224 | 285 | 1.29e-16 | 76.3 |
| MsG0580028994.01.T01 | AT5G22240 | 43.023 | 86 | 45 | 1 | 91 | 176 | 90 | 171 | 6.69e-16 | 72.4 |
| MsG0580028994.01.T01 | AT1G06920 | 40.580 | 69 | 41 | 0 | 94 | 162 | 240 | 308 | 5.99e-13 | 66.2 |
Find 33 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGAAGAAGTGAGGGTATT+AGG | 0.192110 | 5:+90064752 | None:intergenic |
| CCATCACCACAAAGCTTCTT+AGG | 0.222603 | 5:+90064407 | None:intergenic |
| GGGTATTAGGGTTTGTGTTT+TGG | 0.265400 | 5:+90064765 | None:intergenic |
| CTCTATACTTCAAATGATAT+TGG | 0.278958 | 5:-90064552 | MsG0580028994.01.T01:CDS |
| TGTCATTGTTGAAGCGTTCA+TGG | 0.333768 | 5:-90064450 | MsG0580028994.01.T01:CDS |
| GCAACAATAATAATAATTAT+AGG | 0.334536 | 5:-90064655 | MsG0580028994.01.T01:CDS |
| TGGAAGAAGTGAGGGTATTA+GGG | 0.337424 | 5:+90064753 | None:intergenic |
| AGAGGGTTCAAGTACGTTAG+AGG | 0.399454 | 5:+90064811 | None:intergenic |
| ATTGGAGAGAGAGATTTATT+CGG | 0.406374 | 5:-90064534 | MsG0580028994.01.T01:CDS |
| CCTAAGAAGCTTTGTGGTGA+TGG | 0.449802 | 5:-90064407 | MsG0580028994.01.T01:CDS |
| ATATTTCCTAAGAAGCTTTG+TGG | 0.453226 | 5:-90064413 | MsG0580028994.01.T01:CDS |
| TTTGTCCTCGGTGTTGGCAT+CGG | 0.463745 | 5:+90064715 | None:intergenic |
| GAAATCTTTGTCCTCGGTGT+TGG | 0.500764 | 5:+90064709 | None:intergenic |
| GAGAAGTGGTGGAAGAAGTG+AGG | 0.515271 | 5:+90064744 | None:intergenic |
| TTCTCATCTAACAAAAGTTG+TGG | 0.528866 | 5:-90064851 | MsG0580028994.01.T01:CDS |
| AGAAGTGGTGGAAGAAGTGA+GGG | 0.543939 | 5:+90064745 | None:intergenic |
| GAGTTTCGAAAATCTTCATA+CGG | 0.557400 | 5:+90064572 | None:intergenic |
| AGATAGCATTGCCGTTGAGA+AGG | 0.559747 | 5:-90064606 | MsG0580028994.01.T01:CDS |
| GAGGGTTCAAGTACGTTAGA+GGG | 0.563551 | 5:+90064812 | None:intergenic |
| GCTGCTGTTGTCGTTCGAGA+TGG | 0.564449 | 5:+90064676 | None:intergenic |
| ATCGGCGTCGTGAGAAGTGG+TGG | 0.581160 | 5:+90064733 | None:intergenic |
| GTTGTCGTTCGAGATGGTGG+TGG | 0.585183 | 5:+90064682 | None:intergenic |
| TGCCGTTGAGAAGGAATCGA+AGG | 0.586299 | 5:-90064597 | MsG0580028994.01.T01:CDS |
| GCTGTTGTCGTTCGAGATGG+TGG | 0.595976 | 5:+90064679 | None:intergenic |
| GGCATCGGCGTCGTGAGAAG+TGG | 0.597618 | 5:+90064730 | None:intergenic |
| GGAAGTGAAATCTTTGTCCT+CGG | 0.630018 | 5:+90064703 | None:intergenic |
| GAAGGCATTGAAGTCACACA+TGG | 0.632312 | 5:-90064375 | MsG0580028994.01.T01:CDS |
| CTCCTTCGATTCCTTCTCAA+CGG | 0.636372 | 5:+90064595 | None:intergenic |
| TGGTGATGGTAGACGTGTGA+AGG | 0.636762 | 5:-90064393 | MsG0580028994.01.T01:CDS |
| ACGCTTCAACAATGACATGA+TGG | 0.645246 | 5:+90064456 | None:intergenic |
| ATGGGAATTAATGCTGCATG+AGG | 0.647920 | 5:-90064356 | None:intergenic |
| AAGGCATTGAAGTCACACAT+GGG | 0.724296 | 5:-90064374 | MsG0580028994.01.T01:CDS |
| CGACGCCGATGCCAACACCG+AGG | 0.750443 | 5:-90064720 | MsG0580028994.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCAACAATAATAATAATTAT+AGG | - | Chr5:90064596-90064615 | MsG0580028994.01.T01:CDS | 15.0% |
| !!! | TAATTATAGGATTTTAAAGC+CGG | - | Chr5:90064609-90064628 | MsG0580028994.01.T01:CDS | 20.0% |
| ! | CTCTATACTTCAAATGATAT+TGG | - | Chr5:90064699-90064718 | MsG0580028994.01.T01:CDS | 25.0% |
| !! | AACTTTTGTTAGATGAGAAA+AGG | + | Chr5:90064399-90064418 | None:intergenic | 25.0% |
| !! | ACTTTTGTTAGATGAGAAAA+GGG | + | Chr5:90064398-90064417 | None:intergenic | 25.0% |
| !! | TTTTGGTAAAAGGAGATTTT+AGG | + | Chr5:90064472-90064491 | None:intergenic | 25.0% |
| !!! | AATTATAGGATTTTAAAGCC+GGG | - | Chr5:90064610-90064629 | MsG0580028994.01.T01:CDS | 25.0% |
| ATATTTCCTAAGAAGCTTTG+TGG | - | Chr5:90064838-90064857 | MsG0580028994.01.T01:CDS | 30.0% | |
| ATTGGAGAGAGAGATTTATT+CGG | - | Chr5:90064717-90064736 | MsG0580028994.01.T01:CDS | 30.0% | |
| GAGTTTCGAAAATCTTCATA+CGG | + | Chr5:90064682-90064701 | None:intergenic | 30.0% | |
| TTCTCATCTAACAAAAGTTG+TGG | - | Chr5:90064400-90064419 | MsG0580028994.01.T01:CDS | 30.0% | |
| !!! | GAGATTTTAGGTTTTTGAGA+GGG | + | Chr5:90064460-90064479 | None:intergenic | 30.0% |
| !! | CTATCTATGAGTTTTGTACC+CGG | + | Chr5:90064631-90064650 | None:intergenic | 35.0% |
| !!! | AGGGTTTGTGTTTTGGTAAA+AGG | + | Chr5:90064482-90064501 | None:intergenic | 35.0% |
| !!! | GGAGATTTTAGGTTTTTGAG+AGG | + | Chr5:90064461-90064480 | None:intergenic | 35.0% |
| ACGCTTCAACAATGACATGA+TGG | + | Chr5:90064798-90064817 | None:intergenic | 40.0% | |
| GGAAGTGAAATCTTTGTCCT+CGG | + | Chr5:90064551-90064570 | None:intergenic | 40.0% | |
| ! | GGGTATTAGGGTTTGTGTTT+TGG | + | Chr5:90064489-90064508 | None:intergenic | 40.0% |
| ! | TGTCATTGTTGAAGCGTTCA+TGG | - | Chr5:90064801-90064820 | MsG0580028994.01.T01:CDS | 40.0% |
| !! | AAGGCATTGAAGTCACACAT+GGG | - | Chr5:90064877-90064896 | MsG0580028994.01.T01:CDS | 40.0% |
| !! | ATGAGAAAAGGGTTTTGACG+AGG | + | Chr5:90064387-90064406 | None:intergenic | 40.0% |
| !! | TGGAAGAAGTGAGGGTATTA+GGG | + | Chr5:90064501-90064520 | None:intergenic | 40.0% |
| AGAGGGTTCAAGTACGTTAG+AGG | + | Chr5:90064443-90064462 | None:intergenic | 45.0% | |
| AGATAGCATTGCCGTTGAGA+AGG | - | Chr5:90064645-90064664 | MsG0580028994.01.T01:CDS | 45.0% | |
| CCATCACCACAAAGCTTCTT+AGG | + | Chr5:90064847-90064866 | None:intergenic | 45.0% | |
| CTCCTTCGATTCCTTCTCAA+CGG | + | Chr5:90064659-90064678 | None:intergenic | 45.0% | |
| GAGGGTTCAAGTACGTTAGA+GGG | + | Chr5:90064442-90064461 | None:intergenic | 45.0% | |
| ! | AGAAGTGGTGGAAGAAGTGA+GGG | + | Chr5:90064509-90064528 | None:intergenic | 45.0% |
| ! | CCTAAGAAGCTTTGTGGTGA+TGG | - | Chr5:90064844-90064863 | MsG0580028994.01.T01:CDS | 45.0% |
| ! | GAAATCTTTGTCCTCGGTGT+TGG | + | Chr5:90064545-90064564 | None:intergenic | 45.0% |
| !! | GAAGGCATTGAAGTCACACA+TGG | - | Chr5:90064876-90064895 | MsG0580028994.01.T01:CDS | 45.0% |
| !! | GTGGAAGAAGTGAGGGTATT+AGG | + | Chr5:90064502-90064521 | None:intergenic | 45.0% |
| TGCCGTTGAGAAGGAATCGA+AGG | - | Chr5:90064654-90064673 | MsG0580028994.01.T01:CDS | 50.0% | |
| ! | GAGAAGTGGTGGAAGAAGTG+AGG | + | Chr5:90064510-90064529 | None:intergenic | 50.0% |
| !! | TGGTGATGGTAGACGTGTGA+AGG | - | Chr5:90064858-90064877 | MsG0580028994.01.T01:CDS | 50.0% |
| !! | TTTGTCCTCGGTGTTGGCAT+CGG | + | Chr5:90064539-90064558 | None:intergenic | 50.0% |
| GCTGCTGTTGTCGTTCGAGA+TGG | + | Chr5:90064578-90064597 | None:intergenic | 55.0% | |
| GCTGTTGTCGTTCGAGATGG+TGG | + | Chr5:90064575-90064594 | None:intergenic | 55.0% | |
| ! | GTTGTCGTTCGAGATGGTGG+TGG | + | Chr5:90064572-90064591 | None:intergenic | 55.0% |
| ATCGGCGTCGTGAGAAGTGG+TGG | + | Chr5:90064521-90064540 | None:intergenic | 60.0% | |
| !! | GGCATCGGCGTCGTGAGAAG+TGG | + | Chr5:90064524-90064543 | None:intergenic | 65.0% |
| CGACGCCGATGCCAACACCG+AGG | - | Chr5:90064531-90064550 | MsG0580028994.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 90064358 | 90064915 | 90064358 | ID=MsG0580028994.01;Name=MsG0580028994.01 |
| Chr5 | mRNA | 90064358 | 90064915 | 90064358 | ID=MsG0580028994.01.T01;Parent=MsG0580028994.01;Name=MsG0580028994.01.T01;_AED=0.49;_eAED=0.53;_QI=0|-1|0|1|-1|1|1|0|185 |
| Chr5 | exon | 90064358 | 90064915 | 90064358 | ID=MsG0580028994.01.T01:exon:26091;Parent=MsG0580028994.01.T01 |
| Chr5 | CDS | 90064358 | 90064915 | 90064358 | ID=MsG0580028994.01.T01:cds;Parent=MsG0580028994.01.T01 |
| Gene Sequence |
| Protein sequence |
