Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029156.01.T01 | XP_003616581.1 | 98.378 | 185 | 1 | 2 | 1 | 184 | 1 | 184 | 5.72E-131 | 375 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029156.01.T01 | Q8H1D2 | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 4.04E-60 | 188 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029156.01.T01 | G7KBY3 | 98.378 | 185 | 1 | 2 | 1 | 184 | 1 | 184 | 2.73e-131 | 375 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580029156.01.T01 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029156.01.T01 | MTR_5g081990 | 98.378 | 185 | 1 | 2 | 1 | 184 | 1 | 184 | 6.93e-135 | 375 |
| MsG0580029156.01.T01 | MTR_7g060630 | 69.841 | 63 | 19 | 0 | 33 | 95 | 8 | 70 | 1.07e-27 | 103 |
| MsG0580029156.01.T01 | MTR_4g084550 | 61.765 | 68 | 26 | 0 | 33 | 100 | 64 | 131 | 9.35e-27 | 100 |
| MsG0580029156.01.T01 | MTR_8g107210 | 71.429 | 63 | 18 | 0 | 33 | 95 | 96 | 158 | 2.37e-26 | 103 |
| MsG0580029156.01.T01 | MTR_1g019130 | 65.152 | 66 | 23 | 0 | 33 | 98 | 90 | 155 | 7.69e-26 | 99.4 |
| MsG0580029156.01.T01 | MTR_1g019130 | 65.152 | 66 | 23 | 0 | 33 | 98 | 90 | 155 | 9.63e-26 | 99.4 |
| MsG0580029156.01.T01 | MTR_5g021930 | 59.459 | 74 | 26 | 2 | 33 | 105 | 30 | 100 | 1.72e-24 | 97.4 |
| MsG0580029156.01.T01 | MTR_4g063735 | 57.576 | 66 | 28 | 0 | 33 | 98 | 25 | 90 | 1.33e-23 | 94.4 |
| MsG0580029156.01.T01 | MTR_8g095580 | 51.163 | 86 | 40 | 1 | 15 | 98 | 62 | 147 | 4.11e-20 | 82.8 |
| MsG0580029156.01.T01 | MTR_3g088485 | 67.273 | 55 | 18 | 0 | 44 | 98 | 91 | 145 | 1.04e-19 | 81.6 |
| MsG0580029156.01.T01 | MTR_2g015000 | 45.312 | 64 | 35 | 0 | 33 | 96 | 55 | 118 | 2.64e-15 | 73.2 |
| MsG0580029156.01.T01 | MTR_7g025010 | 43.750 | 64 | 36 | 0 | 33 | 96 | 47 | 110 | 2.93e-15 | 73.2 |
| MsG0580029156.01.T01 | MTR_4g088070 | 40.984 | 61 | 36 | 0 | 33 | 93 | 29 | 89 | 3.13e-13 | 67.4 |
| MsG0580029156.01.T01 | MTR_1g115315 | 43.333 | 60 | 34 | 0 | 33 | 92 | 85 | 144 | 1.04e-11 | 62.0 |
| MsG0580029156.01.T01 | MTR_1g115315 | 43.333 | 60 | 34 | 0 | 33 | 92 | 85 | 144 | 1.26e-11 | 62.0 |
| MsG0580029156.01.T01 | MTR_4g088080 | 39.706 | 68 | 39 | 1 | 33 | 100 | 40 | 105 | 4.86e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029156.01.T01 | AT3G11260 | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 4.12e-61 | 188 |
| MsG0580029156.01.T01 | AT5G05770 | 65.487 | 113 | 31 | 3 | 4 | 110 | 2 | 112 | 1.05e-40 | 134 |
| MsG0580029156.01.T01 | AT3G18010 | 71.429 | 63 | 18 | 0 | 33 | 95 | 76 | 138 | 7.69e-27 | 104 |
| MsG0580029156.01.T01 | AT2G28610 | 65.079 | 63 | 22 | 0 | 33 | 95 | 8 | 70 | 6.12e-26 | 100 |
| MsG0580029156.01.T01 | AT5G59340 | 57.333 | 75 | 27 | 1 | 21 | 95 | 7 | 76 | 2.84e-25 | 98.6 |
| MsG0580029156.01.T01 | AT1G46480 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 1.18e-24 | 95.5 |
| MsG0580029156.01.T01 | AT1G46480 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 6.53e-24 | 94.7 |
| MsG0580029156.01.T01 | AT2G17950 | 66.667 | 63 | 20 | 1 | 33 | 94 | 38 | 100 | 9.69e-24 | 95.1 |
| MsG0580029156.01.T01 | AT2G01500 | 67.273 | 55 | 18 | 0 | 33 | 87 | 61 | 115 | 1.16e-21 | 89.4 |
| MsG0580029156.01.T01 | AT2G33880 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 5.88e-15 | 72.0 |
| MsG0580029156.01.T01 | AT2G33880 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 7.03e-15 | 72.0 |
| MsG0580029156.01.T01 | AT5G45980 | 46.774 | 62 | 33 | 0 | 33 | 94 | 55 | 116 | 1.79e-14 | 70.5 |
| MsG0580029156.01.T01 | AT4G35550 | 42.623 | 61 | 35 | 0 | 33 | 93 | 99 | 159 | 5.72e-12 | 63.2 |
| MsG0580029156.01.T01 | AT1G20700 | 35.556 | 90 | 55 | 2 | 19 | 106 | 20 | 108 | 6.25e-12 | 60.8 |
| MsG0580029156.01.T01 | AT1G20700 | 35.556 | 90 | 55 | 2 | 19 | 106 | 82 | 170 | 1.24e-11 | 61.2 |
| MsG0580029156.01.T01 | AT1G20700 | 38.667 | 75 | 45 | 1 | 19 | 93 | 82 | 155 | 1.25e-11 | 61.2 |
Find 25 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCAAGAATGTGTTCTAT+TGG | 0.384217 | 5:-93004081 | MsG0580029156.01.T01:CDS |
| GATTCACCAAATGAGTTCAA+TGG | 0.392997 | 5:+93003626 | None:intergenic |
| AGTTTAACTTGTTCTGTTGT+GGG | 0.394220 | 5:+93004196 | None:intergenic |
| AGAGTCTCAATCACTCTATC+AGG | 0.412077 | 5:+93003659 | None:intergenic |
| ATGTACAATGGGAGAACAAA+TGG | 0.428304 | 5:-93003540 | MsG0580029156.01.T01:CDS |
| AACACCCACCATTGGACTTA+CGG | 0.439714 | 5:-93003517 | MsG0580029156.01.T01:CDS |
| GGTTTCAGAATCACAAAGCT+AGG | 0.453724 | 5:-93004060 | MsG0580029156.01.T01:CDS |
| AAGTTTAACTTGTTCTGTTG+TGG | 0.462988 | 5:+93004195 | None:intergenic |
| TTCTGAATTTGATCAGTGCT+TGG | 0.470346 | 5:+93004139 | None:intergenic |
| AGTGGAAGCAGCAGCAGCAC+TGG | 0.493876 | 5:-93004241 | MsG0580029156.01.T01:CDS |
| AATATGGAAGAGAGCATGTC+AGG | 0.497698 | 5:-93004304 | MsG0580029156.01.T01:CDS |
| TTTGCATACACATGTACAAT+GGG | 0.499345 | 5:-93003551 | MsG0580029156.01.T01:CDS |
| AGTAGTTTCCATGGTAAAAG+TGG | 0.503916 | 5:-93004259 | MsG0580029156.01.T01:CDS |
| ACAAATGGAACACCCACCAT+TGG | 0.543359 | 5:-93003525 | MsG0580029156.01.T01:CDS |
| TCATCTGGAAGTAGTTTCCA+TGG | 0.556114 | 5:-93004268 | MsG0580029156.01.T01:CDS |
| GTTTCAGAATCACAAAGCTA+GGG | 0.556495 | 5:-93004059 | MsG0580029156.01.T01:CDS |
| GTATGCATGCAAATGAATGC+AGG | 0.569996 | 5:-93003586 | MsG0580029156.01.T01:CDS |
| AGCAGCACTGGCACAAAGTG+TGG | 0.575447 | 5:-93004229 | MsG0580029156.01.T01:CDS |
| CTCAACCGTAAGTCCAATGG+TGG | 0.576156 | 5:+93003512 | None:intergenic |
| GTGAATCAGAATCAGAGAAG+TGG | 0.590084 | 5:-93003610 | MsG0580029156.01.T01:CDS |
| CTGGCACAAAGTGTGGTCGT+TGG | 0.591246 | 5:-93004222 | MsG0580029156.01.T01:CDS |
| AAACTCAACCGTAAGTCCAA+TGG | 0.615710 | 5:+93003509 | None:intergenic |
| GTTTGCATACACATGTACAA+TGG | 0.618786 | 5:-93003552 | MsG0580029156.01.T01:CDS |
| TATGCATGCAAATGAATGCA+GGG | 0.627141 | 5:-93003585 | MsG0580029156.01.T01:CDS |
| TCAACCGTAAGTCCAATGGT+GGG | 0.711399 | 5:+93003513 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | Chr5:93003979-93003998 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | Chr5:93004024-93004043 | MsG0580029156.01.T01:CDS | 20.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | - | Chr5:93003691-93003710 | MsG0580029156.01.T01:intron | 25.0% |
| AATGAATAAATGAGTTGCGT+CGG | + | Chr5:93003885-93003904 | None:intergenic | 30.0% | |
| AGTATCAGATCAAAGTTTCA+AGG | + | Chr5:93003925-93003944 | None:intergenic | 30.0% | |
| TTTGCATACACATGTACAAT+GGG | - | Chr5:93004252-93004271 | MsG0580029156.01.T01:CDS | 30.0% | |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | Chr5:93004171-93004190 | MsG0580029156.01.T01:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | Chr5:93004100-93004119 | MsG0580029156.01.T01:CDS | 30.0% |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | Chr5:93004099-93004118 | MsG0580029156.01.T01:CDS | 30.0% |
| !! | AAGTTTAACTTGTTCTGTTG+TGG | + | Chr5:93003611-93003630 | None:intergenic | 30.0% |
| !! | AGTTTAACTTGTTCTGTTGT+GGG | + | Chr5:93003610-93003629 | None:intergenic | 30.0% |
| !! | GCCATAAAAACTAAGTTGAT+TGG | + | Chr5:93003695-93003714 | None:intergenic | 30.0% |
| AGAGCAAGAATGTGTTCTAT+TGG | - | Chr5:93003722-93003741 | MsG0580029156.01.T01:intron | 35.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | Chr5:93003544-93003563 | MsG0580029156.01.T01:CDS | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | Chr5:93004072-93004091 | None:intergenic | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | Chr5:93004263-93004282 | MsG0580029156.01.T01:CDS | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | Chr5:93004180-93004199 | None:intergenic | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | Chr5:93003744-93003763 | MsG0580029156.01.T01:intron | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | Chr5:93004251-93004270 | MsG0580029156.01.T01:CDS | 35.0% | |
| TACATGTTACATCATCGTCA+GGG | + | Chr5:93004071-93004090 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | Chr5:93004218-93004237 | MsG0580029156.01.T01:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | Chr5:93003667-93003686 | None:intergenic | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | Chr5:93003786-93003805 | MsG0580029156.01.T01:intron | 35.0% |
| AAACTCAACCGTAAGTCCAA+TGG | + | Chr5:93004297-93004316 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | Chr5:93004147-93004166 | None:intergenic | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | Chr5:93004109-93004128 | None:intergenic | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | Chr5:93004040-93004059 | MsG0580029156.01.T01:CDS | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | Chr5:93003765-93003784 | MsG0580029156.01.T01:intron | 40.0% | |
| GGTTTCAGAATCACAAAGCT+AGG | - | Chr5:93003743-93003762 | MsG0580029156.01.T01:intron | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | Chr5:93004217-93004236 | MsG0580029156.01.T01:CDS | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | Chr5:93004193-93004212 | MsG0580029156.01.T01:CDS | 40.0% | |
| TCATCTGGAAGTAGTTTCCA+TGG | - | Chr5:93003535-93003554 | MsG0580029156.01.T01:CDS | 40.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | Chr5:93003520-93003539 | MsG0580029156.01.T01:CDS | 40.0% |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | Chr5:93003632-93003651 | MsG0580029156.01.T01:CDS | 40.0% |
| AACACCCACCATTGGACTTA+CGG | - | Chr5:93004286-93004305 | MsG0580029156.01.T01:CDS | 45.0% | |
| ACAAATGGAACACCCACCAT+TGG | - | Chr5:93004278-93004297 | MsG0580029156.01.T01:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | Chr5:93004293-93004312 | None:intergenic | 45.0% | |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | Chr5:93003631-93003650 | MsG0580029156.01.T01:CDS | 45.0% |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | Chr5:93003555-93003574 | None:intergenic | 45.0% |
| !!! | GTTAAAAAAATTATTTTAAA+AGG | - | Chr5:93004000-93004019 | MsG0580029156.01.T01:CDS | 5.0% |
| CTCAACCGTAAGTCCAATGG+TGG | + | Chr5:93004294-93004313 | None:intergenic | 50.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | - | Chr5:93004045-93004064 | MsG0580029156.01.T01:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | Chr5:93004067-93004086 | None:intergenic | 50.0% | |
| CTGGCACAAAGTGTGGTCGT+TGG | - | Chr5:93003581-93003600 | MsG0580029156.01.T01:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | Chr5:93004050-93004069 | None:intergenic | 55.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | Chr5:93003574-93003593 | MsG0580029156.01.T01:CDS | 55.0% |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | Chr5:93003562-93003581 | MsG0580029156.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 93003502 | 93004323 | 93003502 | ID=MsG0580029156.01;Name=MsG0580029156.01 |
| Chr5 | mRNA | 93003502 | 93004323 | 93003502 | ID=MsG0580029156.01.T01;Parent=MsG0580029156.01;Name=MsG0580029156.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|184 |
| Chr5 | exon | 93003972 | 93004323 | 93003972 | ID=MsG0580029156.01.T01:exon:49601;Parent=MsG0580029156.01.T01 |
| Chr5 | exon | 93003502 | 93003704 | 93003502 | ID=MsG0580029156.01.T01:exon:49600;Parent=MsG0580029156.01.T01 |
| Chr5 | CDS | 93003972 | 93004323 | 93003972 | ID=MsG0580029156.01.T01:cds;Parent=MsG0580029156.01.T01 |
| Chr5 | CDS | 93003502 | 93003704 | 93003502 | ID=MsG0580029156.01.T01:cds;Parent=MsG0580029156.01.T01 |
| Gene Sequence |
| Protein sequence |
