AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029167.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029167.01.T01 MTR_5g082140 90.217 460 43 1 27 484 1 460 0.0 821
MsG0580029167.01.T01 MTR_2g024430 53.254 507 184 8 25 482 7 509 3.92e-175 502
MsG0580029167.01.T01 MTR_5g040880 52.353 510 179 10 34 484 7 511 5.43e-166 479
MsG0580029167.01.T01 MTR_5g040740 54.409 465 142 7 27 484 1 402 3.22e-158 455
MsG0580029167.01.T01 MTR_5g061220 50.192 520 183 10 32 484 13 523 2.09e-157 457
MsG0580029167.01.T01 MTR_1g058210 47.072 461 206 11 27 474 1 436 1.90e-131 389
MsG0580029167.01.T01 MTR_2g006270 44.754 467 219 14 30 482 5 446 3.18e-111 337
MsG0580029167.01.T01 MTR_3g073420 40.693 462 216 8 26 437 7 460 2.69e-99 310
MsG0580029167.01.T01 MTR_2g006380 42.026 464 225 17 32 482 14 446 2.97e-98 303
MsG0580029167.01.T01 MTR_5g060630 39.692 519 219 16 31 462 5 516 3.52e-98 305
MsG0580029167.01.T01 MTR_5g460740 39.692 519 219 16 31 462 5 516 3.52e-98 305
MsG0580029167.01.T01 MTR_5g061890 47.813 343 154 7 32 361 6 336 3.07e-94 291
MsG0580029167.01.T01 MTR_5g060780 47.143 350 159 9 32 367 6 343 5.32e-94 290
MsG0580029167.01.T01 MTR_5g460920 47.143 350 159 9 32 367 6 343 2.67e-93 289
MsG0580029167.01.T01 MTR_2g094570 36.673 499 232 13 5 437 35 515 1.04e-89 290
MsG0580029167.01.T01 MTR_2g094570 37.953 469 208 12 35 437 8 459 1.07e-89 287
MsG0580029167.01.T01 MTR_2g094570 37.953 469 208 12 35 437 8 459 1.09e-89 288
MsG0580029167.01.T01 MTR_1g064430 37.757 437 197 12 35 408 24 448 1.67e-81 266
MsG0580029167.01.T01 MTR_4g058930 37.330 442 204 11 27 407 1 430 1.89e-81 266
MsG0580029167.01.T01 MTR_1g064430 37.757 437 197 12 35 408 37 461 3.07e-81 266
MsG0580029167.01.T01 MTR_4g058930 37.330 442 204 11 27 407 1 430 3.21e-81 266
MsG0580029167.01.T01 MTR_1g094960 39.012 405 176 11 35 382 16 406 1.29e-79 260
MsG0580029167.01.T01 MTR_7g101280 36.986 438 198 13 35 406 28 453 3.15e-77 254
MsG0580029167.01.T01 MTR_0041s0110 36.986 438 198 13 35 406 28 453 3.15e-77 254
MsG0580029167.01.T01 MTR_7g101275 40.064 312 154 9 48 339 2 300 1.83e-63 211
MsG0580029167.01.T01 MTR_0041s0120 38.182 330 157 10 48 344 2 317 9.87e-59 199
MsG0580029167.01.T01 MTR_2g018690 34.646 381 212 12 17 373 3 370 2.68e-58 207
MsG0580029167.01.T01 MTR_2g043250 35.429 350 192 10 35 362 20 357 3.94e-58 207
MsG0580029167.01.T01 MTR_5g062970 47.085 223 111 4 10 228 9 228 1.31e-57 191
MsG0580029167.01.T01 MTR_5g076270 34.605 367 196 12 35 373 21 371 1.78e-57 204
MsG0580029167.01.T01 MTR_4g124900 36.103 349 191 10 35 362 20 357 2.21e-57 206
MsG0580029167.01.T01 MTR_4g124900 36.103 349 191 10 35 362 20 357 2.30e-57 205
MsG0580029167.01.T01 MTR_3g064050 33.875 369 196 11 35 373 23 373 4.12e-56 200
MsG0580029167.01.T01 MTR_8g079492 34.121 381 213 12 17 373 3 369 8.47e-56 200
MsG0580029167.01.T01 MTR_8g079492 34.121 381 213 12 17 373 3 369 9.74e-56 200
MsG0580029167.01.T01 MTR_4g021580 30.047 426 254 10 36 440 17 419 3.27e-55 195
MsG0580029167.01.T01 MTR_2g014770 33.243 367 207 11 18 360 35 387 3.74e-55 194
MsG0580029167.01.T01 MTR_4g021580 30.047 426 254 10 36 440 17 419 6.61e-55 195
MsG0580029167.01.T01 MTR_2g014770 33.243 367 207 11 18 360 35 387 1.04e-54 194
MsG0580029167.01.T01 MTR_2g093740 34.140 372 210 12 38 381 56 420 1.85e-53 191
MsG0580029167.01.T01 MTR_8g100050 33.889 360 215 9 37 379 58 411 3.03e-53 191
MsG0580029167.01.T01 MTR_2g093740 34.140 372 210 12 38 381 56 420 3.46e-53 191
MsG0580029167.01.T01 MTR_1g024025 33.333 378 210 12 35 393 40 394 3.78e-53 193
MsG0580029167.01.T01 MTR_2g093740 34.140 372 210 12 38 381 56 420 6.67e-53 191
MsG0580029167.01.T01 MTR_8g100050 33.889 360 215 9 37 379 58 411 1.21e-52 191
MsG0580029167.01.T01 MTR_8g101360 33.241 361 213 8 23 363 16 368 2.10e-51 188
MsG0580029167.01.T01 MTR_7g062540 32.948 346 209 8 38 367 14 352 3.42e-51 185
MsG0580029167.01.T01 MTR_2g093740 32.987 385 210 13 38 381 56 433 8.05e-50 181
MsG0580029167.01.T01 MTR_2g093740 32.987 385 210 13 38 381 56 433 1.23e-49 181
MsG0580029167.01.T01 MTR_2g093740 32.987 385 210 13 38 381 56 433 1.74e-49 181
MsG0580029167.01.T01 MTR_4g060460 34.426 366 208 9 38 377 54 413 7.28e-49 179
MsG0580029167.01.T01 MTR_4g060460 34.426 366 208 9 38 377 54 413 8.64e-49 179
MsG0580029167.01.T01 MTR_8g027440 32.853 347 208 9 38 368 29 366 1.25e-48 177
MsG0580029167.01.T01 MTR_4g088210 32.194 351 198 11 38 360 38 376 2.40e-47 174
MsG0580029167.01.T01 MTR_4g088210 32.194 351 198 11 38 360 38 376 2.41e-47 174
MsG0580029167.01.T01 MTR_2g005240 31.755 359 221 8 37 379 22 372 2.96e-46 171
MsG0580029167.01.T01 MTR_5g060770 33.426 359 173 10 86 428 6 314 3.50e-45 162
MsG0580029167.01.T01 MTR_8g079492 34.899 298 159 10 98 373 3 287 5.91e-44 166
MsG0580029167.01.T01 MTR_2g093740 36.187 257 143 7 140 381 40 290 1.62e-39 151
MsG0580029167.01.T01 MTR_2g093740 36.187 257 143 7 140 381 40 290 3.69e-39 150
MsG0580029167.01.T01 MTR_2g093740 34.444 270 143 8 140 381 40 303 3.64e-36 142
MsG0580029167.01.T01 MTR_2g093740 34.444 270 143 8 140 381 40 303 6.62e-36 141
MsG0580029167.01.T01 MTR_5g062960 43.814 194 78 5 257 441 1 172 2.72e-35 130
MsG0580029167.01.T01 MTR_5g460910 30.278 360 162 12 77 419 13 300 2.32e-30 120
MsG0580029167.01.T01 MTR_8g446900 27.469 324 199 11 87 389 12 320 2.97e-26 110
MsG0580029167.01.T01 MTR_5g046120 38.333 120 65 3 70 184 6 121 2.60e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029167.01.T01 AT1G77850 35.753 551 240 13 35 484 16 553 5.31e-95 299
MsG0580029167.01.T01 AT1G77850 35.753 551 240 13 35 484 16 553 5.95e-95 299
MsG0580029167.01.T01 AT4G30080 38.128 438 190 11 23 396 4 424 1.39e-84 274
MsG0580029167.01.T01 AT2G28350 38.971 408 179 10 35 382 7 404 2.10e-79 260
MsG0580029167.01.T01 AT2G28350 38.971 408 179 10 35 382 7 404 3.69e-79 260
MsG0580029167.01.T01 AT5G37020 34.795 365 198 12 35 373 19 369 5.98e-60 210
MsG0580029167.01.T01 AT5G37020 35.068 365 197 12 35 373 19 369 1.32e-59 210
MsG0580029167.01.T01 AT1G30330 34.286 385 208 11 17 373 3 370 9.69e-58 206
MsG0580029167.01.T01 AT1G30330 34.115 384 212 10 17 373 3 372 1.29e-57 205
MsG0580029167.01.T01 AT1G30330 34.115 384 212 10 17 373 3 372 1.29e-57 205
MsG0580029167.01.T01 AT2G33860 33.162 389 213 12 38 399 54 422 4.26e-55 194
MsG0580029167.01.T01 AT1G19220 34.426 366 205 11 19 362 5 357 1.05e-54 197
MsG0580029167.01.T01 AT5G20730 34.060 367 208 10 18 362 5 359 1.70e-54 197
MsG0580029167.01.T01 AT5G20730 34.060 367 208 10 18 362 5 359 1.82e-54 197
MsG0580029167.01.T01 AT5G20730 34.332 367 206 11 18 362 5 358 2.61e-54 197
MsG0580029167.01.T01 AT4G23980 31.903 373 224 10 38 390 12 374 1.76e-53 190
MsG0580029167.01.T01 AT1G19850 35.262 363 204 11 30 373 36 386 6.36e-53 192
MsG0580029167.01.T01 AT4G23980 31.978 369 221 10 38 386 12 370 6.51e-53 189
MsG0580029167.01.T01 AT1G19850 35.262 363 204 11 30 373 47 397 7.87e-53 192
MsG0580029167.01.T01 AT5G60450 34.828 379 205 13 33 377 28 398 2.65e-52 189
MsG0580029167.01.T01 AT5G60450 35.246 366 203 12 38 377 65 422 7.06e-52 188
MsG0580029167.01.T01 AT5G62000 32.143 392 241 9 37 409 60 445 8.52e-52 188
MsG0580029167.01.T01 AT5G62000 32.143 392 241 9 37 409 60 445 9.01e-52 188
MsG0580029167.01.T01 AT5G62000 32.143 392 241 9 37 409 60 445 9.01e-52 188
MsG0580029167.01.T01 AT5G62000 32.143 392 241 9 37 409 60 445 9.01e-52 188
MsG0580029167.01.T01 AT5G62000 32.143 392 241 9 37 409 60 445 9.01e-52 188
MsG0580029167.01.T01 AT2G46530 32.312 359 212 9 38 377 21 367 1.35e-51 184
MsG0580029167.01.T01 AT2G46530 32.479 351 206 9 38 369 42 380 2.90e-51 184
MsG0580029167.01.T01 AT3G61830 32.967 364 209 12 38 379 25 375 3.18e-51 184
MsG0580029167.01.T01 AT1G35520 33.151 365 202 12 38 379 27 372 2.64e-47 172
MsG0580029167.01.T01 AT1G34310 29.935 461 275 13 35 466 24 465 7.13e-47 171
MsG0580029167.01.T01 AT1G59750 30.978 368 214 10 37 379 21 373 8.09e-47 172
MsG0580029167.01.T01 AT1G59750 30.978 368 214 10 37 379 21 373 8.20e-47 172
MsG0580029167.01.T01 AT1G59750 30.978 368 214 10 37 379 21 373 8.20e-47 172
MsG0580029167.01.T01 AT1G59750 30.978 368 214 10 37 379 21 373 9.40e-47 172
MsG0580029167.01.T01 AT1G34410 32.764 351 197 11 38 368 27 358 2.26e-46 170
MsG0580029167.01.T01 AT1G35240 29.556 450 273 13 38 467 27 452 3.01e-46 170
MsG0580029167.01.T01 AT1G35540 31.287 342 200 8 38 360 27 352 9.12e-45 166
MsG0580029167.01.T01 AT1G34170 32.486 354 192 12 33 360 20 352 1.45e-44 164
MsG0580029167.01.T01 AT1G34390 31.781 365 205 12 38 379 27 370 4.15e-44 164
MsG0580029167.01.T01 AT5G37020 36.220 254 138 8 140 377 33 278 5.87e-44 165
MsG0580029167.01.T01 AT2G33860 33.574 277 152 9 140 399 4 265 8.56e-41 152
MsG0580029167.01.T01 AT3G61830 32.907 313 180 10 85 379 24 324 1.97e-40 153
MsG0580029167.01.T01 AT1G34170 31.988 347 177 12 33 360 20 326 2.20e-38 146
MsG0580029167.01.T01 AT1G34170 31.988 347 177 12 33 360 20 326 6.49e-38 146
MsG0580029167.01.T01 AT2G46530 32.922 243 141 6 140 369 39 272 5.20e-36 140
MsG0580029167.01.T01 AT1G43950 33.333 213 116 8 38 241 27 222 6.45e-24 100

Find 110 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTTCTATTAAGCTATT+TGG 0.141873 5:+93135661 MsG0580029167.01.T01:CDS
TCTTAATATCATAGGTTAAC+TGG 0.217841 5:+93135302 MsG0580029167.01.T01:intron
GTTGACACAGAGAAAATTAT+TGG 0.300455 5:+93133789 MsG0580029167.01.T01:CDS
AGGGAGAGTGTCTCGGTGTT+AGG 0.314359 5:-93133372 None:intergenic
AGAAAGCAATGCAAATTAAT+TGG 0.320693 5:+93134030 MsG0580029167.01.T01:CDS
GAAGTACCTGAGACGGCATT+TGG 0.330889 5:-93133671 None:intergenic
TCATCCATGGCGCATGCTTC+AGG 0.334788 5:+93134154 MsG0580029167.01.T01:CDS
CGTTATGGAGGCAGCAGAAT+TGG 0.366432 5:+93133923 MsG0580029167.01.T01:CDS
AAAACTTGAGAATTTAGTTT+CGG 0.371328 5:-93133309 None:intergenic
ATGCAAATTAATTGGCAATC+TGG 0.372298 5:+93134038 MsG0580029167.01.T01:CDS
GAGCGCCAGAAAATATGATT+AGG 0.373290 5:-93133168 None:intergenic
GGCAGCAGAATTGGCGGATA+AGG 0.384504 5:+93133932 MsG0580029167.01.T01:CDS
TACTCCAGTTAGTGGTGATT+AGG 0.404311 5:-93133761 None:intergenic
GACCATAGAGGTTGTTGAAA+AGG 0.408752 5:+93135687 MsG0580029167.01.T01:CDS
TCTCTCTATGTTAGTTGATA+AGG 0.411301 5:-93135452 None:intergenic
GGGAGAGTGTCTCGGTGTTA+GGG 0.417483 5:-93133371 None:intergenic
AGCGCCAGAAAATATGATTA+GGG 0.422758 5:-93133167 None:intergenic
AAGAAATTGAACTCTATCTC+TGG 0.424200 5:-93135484 None:intergenic
GACACCTAATCACCACTAAC+TGG 0.430030 5:+93133757 MsG0580029167.01.T01:CDS
GTCATTATCATTCAAAGAGT+TGG 0.431834 5:-93135590 None:intergenic
ATTATCCGACGGCTAGTCAT+TGG 0.433407 5:+93133976 MsG0580029167.01.T01:CDS
TGCCAAGGACTTACTTGACT+TGG 0.438785 5:-93135349 None:intergenic
GTGGGTTTCTGAGGATCCAT+GGG 0.447035 5:-93133234 None:intergenic
GAGACGGCATTTGGGATTGT+AGG 0.450973 5:-93133662 None:intergenic
GGTGGGTTTCTGAGGATCCA+TGG 0.451620 5:-93133235 None:intergenic
AAAATCTGGCAGTGCTTGGC+CGG 0.457026 5:+93133270 MsG0580029167.01.T01:CDS
GGGAATTGTAGAGGCAGTGC+TGG 0.458275 5:-93135394 None:intergenic
AGTGGTGATTAGGTGTCGCT+TGG 0.461123 5:-93133751 None:intergenic
AACGAACTTACTCCAGTTAG+TGG 0.466764 5:-93133769 None:intergenic
AATGCCGTCTCAGGTACTTC+CGG 0.466944 5:+93133674 MsG0580029167.01.T01:CDS
GCAGTGCTTGGCCGGACCAT+CGG 0.467814 5:+93133278 MsG0580029167.01.T01:CDS
GAAGACGAACAAGGTGGTGA+TGG 0.472136 5:+93133537 MsG0580029167.01.T01:CDS
GGAGACGACGCAGGGAATGA+TGG 0.473784 5:-93133412 None:intergenic
TTACTTTCCACTAGGTCACT+TGG 0.487944 5:+93133332 MsG0580029167.01.T01:CDS
AAGTACCTGAGACGGCATTT+GGG 0.489716 5:-93133670 None:intergenic
TGGGACGGTAACCATTGATA+AGG 0.490688 5:-93133392 None:intergenic
TTGCATGAGTCTCGTATCAA+TGG 0.490704 5:+93135712 MsG0580029167.01.T01:CDS
ATCCAAGTCAAGTAAGTCCT+TGG 0.492391 5:+93135347 MsG0580029167.01.T01:CDS
ACAATCCCAAATGCCGTCTC+AGG 0.502462 5:+93133665 MsG0580029167.01.T01:CDS
CAAAGAGTTGGAAGAAGTTG+AGG 0.511344 5:-93135578 None:intergenic
ATCAATTTCGCGCATTCAGA+AGG 0.518250 5:-93133624 None:intergenic
GATAAAGATGAGATCTTCAT+CGG 0.522227 5:+93133852 MsG0580029167.01.T01:CDS
CTTAATATCATAGGTTAACT+GGG 0.524753 5:+93135303 MsG0580029167.01.T01:intron
CATCATAATTCAACGACGAT+GGG 0.525431 5:-93135510 None:intergenic
AACTTTCGCCACACCTACCG+TGG 0.527055 5:+93133723 MsG0580029167.01.T01:CDS
GTCTCAGGTACTTCCGGTCA+AGG 0.528972 5:+93133680 MsG0580029167.01.T01:CDS
AACTCTATCTCTGGAATGGA+GGG 0.529089 5:-93135475 None:intergenic
GTGGTGATTAGGTGTCGCTT+GGG 0.529316 5:-93133750 None:intergenic
TTCGTGAAAACCATTGGTGA+TGG 0.529499 5:-93133496 None:intergenic
AGTGTCTCGGTGTTAGGGGA+AGG 0.530063 5:-93133366 None:intergenic
ATTGAACTCTATCTCTGGAA+TGG 0.532356 5:-93135479 None:intergenic
GGGACGGTAACCATTGATAA+GGG 0.533931 5:-93133391 None:intergenic
CACCGTTATTAGCATCAGAC+GGG 0.539853 5:-93133587 None:intergenic
TGGGTTTCTGAGGATCCATG+GGG 0.544883 5:-93133233 None:intergenic
AGGAGCTTCGTGAAAACCAT+TGG 0.550219 5:-93133502 None:intergenic
GCTATTTGGTAAGACCATAG+AGG 0.550878 5:+93135675 MsG0580029167.01.T01:CDS
TATCAATGGAGAGGATGGTT+CGG 0.552538 5:+93135726 MsG0580029167.01.T01:CDS
CTGATCCTTCTCATCATCCA+TGG 0.552744 5:+93134141 MsG0580029167.01.T01:CDS
ATCAACGTGGGAGACGACGC+AGG 0.552853 5:-93133421 None:intergenic
GAGACGACGCAGGGAATGAT+GGG 0.554533 5:-93133411 None:intergenic
CAATTCTGCTGCCTCCATAA+CGG 0.556885 5:-93133922 None:intergenic
AAGTGCTGCTGCTAAGATAA+CGG 0.557189 5:+93133893 MsG0580029167.01.T01:CDS
TATGGAGGCAGCAGAATTGG+CGG 0.563383 5:+93133926 MsG0580029167.01.T01:CDS
AAGACGAACAAGGTGGTGAT+GGG 0.566040 5:+93133538 MsG0580029167.01.T01:CDS
GAACTCTATCTCTGGAATGG+AGG 0.568555 5:-93135476 None:intergenic
TGAAGTTATTTATTATCCGA+CGG 0.573203 5:+93133965 MsG0580029167.01.T01:CDS
ACCCCGTCTGATGCTAATAA+CGG 0.573286 5:+93133585 MsG0580029167.01.T01:CDS
TCGTGAAAACCATTGGTGAT+GGG 0.574083 5:-93133495 None:intergenic
GCATCATAATTCAACGACGA+TGG 0.574168 5:-93135511 None:intergenic
AAATTGTGGATGTCCTTGAC+CGG 0.574466 5:-93133693 None:intergenic
GAGACACTCTCCCTTATCAA+TGG 0.574718 5:+93133381 MsG0580029167.01.T01:CDS
CTTCTAACTCCCATCACCAA+TGG 0.575065 5:+93133486 MsG0580029167.01.T01:CDS
GGGTTTCTGAGGATCCATGG+GGG 0.578685 5:-93133232 None:intergenic
TCAACACGGGTGGGTTTCTG+AGG 0.579497 5:-93133243 None:intergenic
GGGATTGTAGGTCAAGTGGC+GGG 0.582541 5:-93133650 None:intergenic
TCTCGTATCAATGGAGAGGA+TGG 0.584544 5:+93135721 MsG0580029167.01.T01:CDS
ATTTGGGATTGTAGGTCAAG+TGG 0.584928 5:-93133654 None:intergenic
AGATCTTCATCGGAATCCGC+AGG 0.586965 5:+93133862 MsG0580029167.01.T01:CDS
GTGTCTCGGTGTTAGGGGAA+GGG 0.590737 5:-93133365 None:intergenic
TGGGAGACCCACGGTAGGTG+TGG 0.592829 5:-93133731 None:intergenic
TTTCGCGCATTCAGAAGGTA+CGG 0.595810 5:-93133619 None:intergenic
TCGTCTTCATGAACCTCCTG+AGG 0.595929 5:-93133522 None:intergenic
ATGCGCCATGGATGATGAGA+AGG 0.597068 5:-93134146 None:intergenic
GCATGTTCCAAGTGACCTAG+TGG 0.597744 5:-93133339 None:intergenic
TCTTTGAATGATAATGACAA+TGG 0.598013 5:+93135595 MsG0580029167.01.T01:CDS
CCACCGTTATTAGCATCAGA+CGG 0.605856 5:-93133588 None:intergenic
CAGCACTTAACTTGTGCCTG+CGG 0.605893 5:-93133878 None:intergenic
CATACCTGAAGCATGCGCCA+TGG 0.607260 5:-93134158 None:intergenic
ATTGATAAGGGAGAGTGTCT+CGG 0.607906 5:-93133379 None:intergenic
GACACAGAGAAAATTATTGG+CGG 0.610636 5:+93133792 MsG0580029167.01.T01:CDS
TGGGATTGTAGGTCAAGTGG+CGG 0.613260 5:-93133651 None:intergenic
GAAAGTTCCAAACAAAATTG+TGG 0.613552 5:-93133707 None:intergenic
GAAATAAGTTCAATCTGCCA+AGG 0.619391 5:-93135364 None:intergenic
GGAGAGTGTCTCGGTGTTAG+GGG 0.624404 5:-93133370 None:intergenic
TAGAGGCAGTGCTGGAGTGT+GGG 0.633339 5:-93135386 None:intergenic
ACTTTCGCCACACCTACCGT+GGG 0.642314 5:+93133724 MsG0580029167.01.T01:CDS
CGACGCAGGGAATGATGGGA+CGG 0.650343 5:-93133407 None:intergenic
GAGGTTCATGAAGACGAACA+AGG 0.654030 5:+93133528 MsG0580029167.01.T01:CDS
TGAGTCTCGTATCAATGGAG+AGG 0.655210 5:+93135717 MsG0580029167.01.T01:CDS
TCGCTTGGGAGACCCACGGT+AGG 0.655585 5:-93133736 None:intergenic
ATCAGCTAAGAGATCAACGT+GGG 0.664891 5:-93133433 None:intergenic
GTAGAGGCAGTGCTGGAGTG+TGG 0.665724 5:-93135387 None:intergenic
TCAACGTGGGAGACGACGCA+GGG 0.667679 5:-93133420 None:intergenic
TTGACCGGAAGTACCTGAGA+CGG 0.673143 5:-93133678 None:intergenic
TTACACCAATGACTAGCCGT+CGG 0.681240 5:-93133981 None:intergenic
GGTGTCGCTTGGGAGACCCA+CGG 0.696022 5:-93133740 None:intergenic
CCGTCTGATGCTAATAACGG+TGG 0.711537 5:+93133588 MsG0580029167.01.T01:CDS
ACCGTTATTAGCATCAGACG+GGG 0.747888 5:-93133586 None:intergenic
GTTCATGAAGACGAACAAGG+TGG 0.759394 5:+93133531 MsG0580029167.01.T01:CDS
GATCAGCTAAGAGATCAACG+TGG 0.824076 5:-93133434 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATTTATGTGCATAAT+AGG + Chr5:93135055-93135074 MsG0580029167.01.T01:intron 15.0%
!! TATTTGTAAAATTATGACTA+GGG + Chr5:93134364-93134383 MsG0580029167.01.T01:intron 15.0%
!! TTATTTGTAAAATTATGACT+AGG + Chr5:93134363-93134382 MsG0580029167.01.T01:intron 15.0%
!!! AGAATTTTTTATTAAAACGA+GGG - Chr5:93133823-93133842 None:intergenic 15.0%
!!! TAGAATTTTTTATTAAAACG+AGG - Chr5:93133824-93133843 None:intergenic 15.0%
!! AGATAATAGGTGTAAATTAA+AGG - Chr5:93134669-93134688 None:intergenic 20.0%
!! GATAATAGGTGTAAATTAAA+GGG - Chr5:93134668-93134687 None:intergenic 20.0%
!! TAAATATAAGAATGTTGTAG+TGG - Chr5:93135197-93135216 None:intergenic 20.0%
!! TACAAATAATGTTATCACAT+TGG - Chr5:93134352-93134371 None:intergenic 20.0%
!! TATGATATTAAGATGAAAAG+TGG - Chr5:93135295-93135314 None:intergenic 20.0%
!! TTCATCTGTAATAACATTTA+TGG - Chr5:93134250-93134269 None:intergenic 20.0%
!!! AAAACTTGAGAATTTAGTTT+CGG - Chr5:93133312-93133331 None:intergenic 20.0%
!!! ACTTTTCATCTTAATATCAT+AGG + Chr5:93135294-93135313 MsG0580029167.01.T01:intron 20.0%
!!! ATCGTTCATTTCTAATTTTT+TGG - Chr5:93134582-93134601 None:intergenic 20.0%
!!! CTCTAAAAAATTGTTTAGAT+GGG - Chr5:93134488-93134507 None:intergenic 20.0%
! AACACCAAAAAAGTTAATCA+TGG + Chr5:93135643-93135662 MsG0580029167.01.T01:CDS 25.0%
! AACTATAAAGTTGTACACAT+TGG + Chr5:93134752-93134771 MsG0580029167.01.T01:intron 25.0%
! AGAAAGCAATGCAAATTAAT+TGG + Chr5:93134030-93134049 MsG0580029167.01.T01:CDS 25.0%
! CTTAATATCATAGGTTAACT+GGG + Chr5:93135303-93135322 MsG0580029167.01.T01:intron 25.0%
! TATTAAGATGAAAAGTGGTA+AGG - Chr5:93135290-93135309 None:intergenic 25.0%
! TCTTAATATCATAGGTTAAC+TGG + Chr5:93135302-93135321 MsG0580029167.01.T01:intron 25.0%
! TGAAGTTATTTATTATCCGA+CGG + Chr5:93133965-93133984 MsG0580029167.01.T01:CDS 25.0%
!! AAAACCCTAATCATATTTTC+TGG + Chr5:93133163-93133182 MsG0580029167.01.T01:CDS 25.0%
!! CCTCTAAAAAATTGTTTAGA+TGG - Chr5:93134489-93134508 None:intergenic 25.0%
!! GTGATTTATACTCCAATTTT+AGG - Chr5:93135006-93135025 None:intergenic 25.0%
!! TCTTTGAATGATAATGACAA+TGG + Chr5:93135595-93135614 MsG0580029167.01.T01:CDS 25.0%
!!! AATTCTAAGCTTTTTTTGTG+GGG - Chr5:93135419-93135438 None:intergenic 25.0%
!!! AGAACCATGATTAACTTTTT+TGG - Chr5:93135650-93135669 None:intergenic 25.0%
!!! CAATTCTAAGCTTTTTTTGT+GGG - Chr5:93135420-93135439 None:intergenic 25.0%
!!! CCATCTAAACAATTTTTTAG+AGG + Chr5:93134486-93134505 MsG0580029167.01.T01:intron 25.0%
AAAAAAGCTTAGAATTGCTC+AGG + Chr5:93135423-93135442 MsG0580029167.01.T01:CDS 30.0%
AAAAAGCTTAGAATTGCTCA+GGG + Chr5:93135424-93135443 MsG0580029167.01.T01:CDS 30.0%
AAGAAATTGAACTCTATCTC+TGG - Chr5:93135487-93135506 None:intergenic 30.0%
AAGATAATTTCACACCTACA+TGG + Chr5:93134977-93134996 MsG0580029167.01.T01:intron 30.0%
ACCTTTGATGTCAATAGAAA+AGG - Chr5:93134835-93134854 None:intergenic 30.0%
ATCTTCAACCCCTTAAATAT+AGG + Chr5:93134701-93134720 MsG0580029167.01.T01:intron 30.0%
ATGCAAATTAATTGGCAATC+TGG + Chr5:93134038-93134057 MsG0580029167.01.T01:CDS 30.0%
GAAAGTTCCAAACAAAATTG+TGG - Chr5:93133710-93133729 None:intergenic 30.0%
GATAAAGATGAGATCTTCAT+CGG + Chr5:93133852-93133871 MsG0580029167.01.T01:CDS 30.0%
GTCATTATCATTCAAAGAGT+TGG - Chr5:93135593-93135612 None:intergenic 30.0%
GTTGACACAGAGAAAATTAT+TGG + Chr5:93133789-93133808 MsG0580029167.01.T01:CDS 30.0%
TATATTTAAGGGGTTGAAGA+TGG - Chr5:93134702-93134721 None:intergenic 30.0%
TCTTCAACCCCTTAAATATA+GGG + Chr5:93134702-93134721 MsG0580029167.01.T01:intron 30.0%
TGGAAGAAACACAAGATAAT+AGG - Chr5:93134682-93134701 None:intergenic 30.0%
TGTATACATAAGACTAGAGT+CGG + Chr5:93134603-93134622 MsG0580029167.01.T01:intron 30.0%
TTGCTTGCTTATATGTACTA+AGG + Chr5:93134878-93134897 MsG0580029167.01.T01:intron 30.0%
! AAGAGAAGCTCAATTTTTCA+AGG + Chr5:93134101-93134120 MsG0580029167.01.T01:CDS 30.0%
! ACCTTTTCTATTGACATCAA+AGG + Chr5:93134831-93134850 MsG0580029167.01.T01:intron 30.0%
! CATGGTTCTATTAAGCTATT+TGG + Chr5:93135661-93135680 MsG0580029167.01.T01:CDS 30.0%
! TAGTCATTGGTGTAATTTTG+TGG + Chr5:93133989-93134008 MsG0580029167.01.T01:CDS 30.0%
! TCTCTCTATGTTAGTTGATA+AGG - Chr5:93135455-93135474 None:intergenic 30.0%
!! AGAGAAGCTCAATTTTTCAA+GGG + Chr5:93134102-93134121 MsG0580029167.01.T01:CDS 30.0%
!! TCAGGTATGATTTCTTGTTT+TGG + Chr5:93134172-93134191 MsG0580029167.01.T01:intron 30.0%
!!! AATGACAATGGTTTTGTTGA+AGG + Chr5:93135607-93135626 MsG0580029167.01.T01:CDS 30.0%
!!! ATTCTAAGCTTTTTTTGTGG+GGG - Chr5:93135418-93135437 None:intergenic 30.0%
!!! CAAGTTTTTTACTTTCCACT+AGG + Chr5:93133324-93133343 MsG0580029167.01.T01:CDS 30.0%
!!! GATTAACTTTTTTGGTGTTG+AGG - Chr5:93135642-93135661 None:intergenic 30.0%
!!! GCAATTCTAAGCTTTTTTTG+TGG - Chr5:93135421-93135440 None:intergenic 30.0%
AACCATGCATCGTCTAATTA+TGG - Chr5:93134289-93134308 None:intergenic 35.0%
AGCGCCAGAAAATATGATTA+GGG - Chr5:93133170-93133189 None:intergenic 35.0%
ATATAAGCAAGCAAAAAGCC+AGG - Chr5:93134872-93134891 None:intergenic 35.0%
CATCATAATTCAACGACGAT+GGG - Chr5:93135513-93135532 None:intergenic 35.0%
CCTAAAACTTAGTACTCGAT+CGG + Chr5:93134926-93134945 MsG0580029167.01.T01:intron 35.0%
GAAATAAGTTCAATCTGCCA+AGG - Chr5:93135367-93135386 None:intergenic 35.0%
GACACAGAGAAAATTATTGG+CGG + Chr5:93133792-93133811 MsG0580029167.01.T01:CDS 35.0%
GGCAGAAAAAAGATTAGGTA+TGG - Chr5:93135560-93135579 None:intergenic 35.0%
GGTACAAGCCCTATATTTAA+GGG - Chr5:93134713-93134732 None:intergenic 35.0%
GTACAAGCCCTATATTTAAG+GGG - Chr5:93134712-93134731 None:intergenic 35.0%
GTTGAGGCAGAAAAAAGATT+AGG - Chr5:93135565-93135584 None:intergenic 35.0%
TGGTACAAGCCCTATATTTA+AGG - Chr5:93134714-93134733 None:intergenic 35.0%
TTACGTACAGATGAAAGAGA+TGG - Chr5:93134734-93134753 None:intergenic 35.0%
TTCCATAATTAGACGATGCA+TGG + Chr5:93134284-93134303 MsG0580029167.01.T01:intron 35.0%
! AGGACATCCACAATTTTGTT+TGG + Chr5:93133700-93133719 MsG0580029167.01.T01:CDS 35.0%
! AGGTTTTTCATAAACCTACC+TGG + Chr5:93134851-93134870 MsG0580029167.01.T01:intron 35.0%
! AGTAGCTTTGCAACTAGTAT+AGG + Chr5:93134319-93134338 MsG0580029167.01.T01:intron 35.0%
! ATCCTTTTCAACAACCTCTA+TGG - Chr5:93135692-93135711 None:intergenic 35.0%
! ATTGAACTCTATCTCTGGAA+TGG - Chr5:93135482-93135501 None:intergenic 35.0%
! CAGTTTTGCAAAGACTTCAT+CGG - Chr5:93133466-93133485 None:intergenic 35.0%
! CGGAATCGACTTATATCTAA+GGG + Chr5:93134623-93134642 MsG0580029167.01.T01:intron 35.0%
! TCATTGGTGTAATTTTGTGG+TGG + Chr5:93133992-93134011 MsG0580029167.01.T01:CDS 35.0%
! TCGGAATCGACTTATATCTA+AGG + Chr5:93134622-93134641 MsG0580029167.01.T01:intron 35.0%
!! GATAACGGAAAAATCCGTTA+TGG + Chr5:93133908-93133927 MsG0580029167.01.T01:CDS 35.0%
!! GCTTTTCTTGATAGCATGAA+GGG + Chr5:93135532-93135551 MsG0580029167.01.T01:CDS 35.0%
!! TGCTTTTCTTGATAGCATGA+AGG + Chr5:93135531-93135550 MsG0580029167.01.T01:CDS 35.0%
!!! TTCTAAGCTTTTTTTGTGGG+GGG - Chr5:93135417-93135436 None:intergenic 35.0%
!!! TTTTTTGGTGTTGAGGTTGT+GGG - Chr5:93135635-93135654 None:intergenic 35.0%
AAATTGTGGATGTCCTTGAC+CGG - Chr5:93133696-93133715 None:intergenic 40.0%
AACGAACTTACTCCAGTTAG+TGG - Chr5:93133772-93133791 None:intergenic 40.0%
AACGGAAAAATCCGTTATGG+AGG + Chr5:93133911-93133930 MsG0580029167.01.T01:CDS 40.0%
AATCAATGACTCTCTCACAG+TGG + Chr5:93134423-93134442 MsG0580029167.01.T01:intron 40.0%
AGGTGTAAATTAAAGGGTCG+AGG - Chr5:93134662-93134681 None:intergenic 40.0%
ATCAATGACTCTCTCACAGT+GGG + Chr5:93134424-93134443 MsG0580029167.01.T01:intron 40.0%
ATCAATTTCGCGCATTCAGA+AGG - Chr5:93133627-93133646 None:intergenic 40.0%
ATCAGCTAAGAGATCAACGT+GGG - Chr5:93133436-93133455 None:intergenic 40.0%
ATCCAAGTCAAGTAAGTCCT+TGG + Chr5:93135347-93135366 MsG0580029167.01.T01:CDS 40.0%
CAAAGAGTTGGAAGAAGTTG+AGG - Chr5:93135581-93135600 None:intergenic 40.0%
CCTACATGGTGTCCTAAAAT+TGG + Chr5:93134991-93135010 MsG0580029167.01.T01:intron 40.0%
GACCATAGAGGTTGTTGAAA+AGG + Chr5:93135687-93135706 MsG0580029167.01.T01:CDS 40.0%
GAGCGCCAGAAAATATGATT+AGG - Chr5:93133171-93133190 None:intergenic 40.0%
GCATCATAATTCAACGACGA+TGG - Chr5:93135514-93135533 None:intergenic 40.0%
GCTATTTGGTAAGACCATAG+AGG + Chr5:93135675-93135694 MsG0580029167.01.T01:CDS 40.0%
GGTGTAAATTAAAGGGTCGA+GGG - Chr5:93134661-93134680 None:intergenic 40.0%
GGTTAATGTCTCTGAGTCAA+TGG - Chr5:93134814-93134833 None:intergenic 40.0%
GTGTAAATTAAAGGGTCGAG+GGG - Chr5:93134660-93134679 None:intergenic 40.0%
TACTCCAGTTAGTGGTGATT+AGG - Chr5:93133764-93133783 None:intergenic 40.0%
TATCAATGGAGAGGATGGTT+CGG + Chr5:93135726-93135745 MsG0580029167.01.T01:CDS 40.0%
TTACTTTCCACTAGGTCACT+TGG + Chr5:93133332-93133351 MsG0580029167.01.T01:CDS 40.0%
TTCGTGAAAACCATTGGTGA+TGG - Chr5:93133499-93133518 None:intergenic 40.0%
TTGCATGAGTCTCGTATCAA+TGG + Chr5:93135712-93135731 MsG0580029167.01.T01:CDS 40.0%
! AAGTGCTGCTGCTAAGATAA+CGG + Chr5:93133893-93133912 MsG0580029167.01.T01:CDS 40.0%
! ATTTGGGATTGTAGGTCAAG+TGG - Chr5:93133657-93133676 None:intergenic 40.0%
! CCGATCGAGTACTAAGTTTT+AGG - Chr5:93134929-93134948 None:intergenic 40.0%
! TCGTGAAAACCATTGGTGAT+GGG - Chr5:93133498-93133517 None:intergenic 40.0%
! TGTGCAGTATGCCATCTTTT+TGG - Chr5:93134788-93134807 None:intergenic 40.0%
!! AACTCTATCTCTGGAATGGA+GGG - Chr5:93135478-93135497 None:intergenic 40.0%
!! AGTTTCGGAATTTTGACCGA+TGG - Chr5:93133297-93133316 None:intergenic 40.0%
!! ATTGATAAGGGAGAGTGTCT+CGG - Chr5:93133382-93133401 None:intergenic 40.0%
!! ATTTTTCGATCAACACGGGT+GGG - Chr5:93133255-93133274 None:intergenic 40.0%
!! CCAGATTTTTCGATCAACAC+GGG - Chr5:93133259-93133278 None:intergenic 40.0%
!! GCCAGATTTTTCGATCAACA+CGG - Chr5:93133260-93133279 None:intergenic 40.0%
!! TACTCGATCGGTCTTATTGT+AGG + Chr5:93134938-93134957 MsG0580029167.01.T01:intron 40.0%
!!! CCAATTTTAGGACACCATGT+AGG - Chr5:93134994-93135013 None:intergenic 40.0%
!!! CTTTTTTGGTGTTGAGGTTG+TGG - Chr5:93135636-93135655 None:intergenic 40.0%
AAGACGAACAAGGTGGTGAT+GGG + Chr5:93133538-93133557 MsG0580029167.01.T01:CDS 45.0%
AAGCAAGCAAAAAGCCAGGT+AGG - Chr5:93134868-93134887 None:intergenic 45.0%
AAGTACCTGAGACGGCATTT+GGG - Chr5:93133673-93133692 None:intergenic 45.0%
ACCCCGTCTGATGCTAATAA+CGG + Chr5:93133585-93133604 MsG0580029167.01.T01:CDS 45.0%
ACCGTTATTAGCATCAGACG+GGG - Chr5:93133589-93133608 None:intergenic 45.0%
AGGAGCTTCGTGAAAACCAT+TGG - Chr5:93133505-93133524 None:intergenic 45.0%
CAATTCTGCTGCCTCCATAA+CGG - Chr5:93133925-93133944 None:intergenic 45.0%
CACCGTTATTAGCATCAGAC+GGG - Chr5:93133590-93133609 None:intergenic 45.0%
CCACCGTTATTAGCATCAGA+CGG - Chr5:93133591-93133610 None:intergenic 45.0%
CTGATCCTTCTCATCATCCA+TGG + Chr5:93134141-93134160 MsG0580029167.01.T01:CDS 45.0%
CTTCTAACTCCCATCACCAA+TGG + Chr5:93133486-93133505 MsG0580029167.01.T01:CDS 45.0%
GACACCTAATCACCACTAAC+TGG + Chr5:93133757-93133776 MsG0580029167.01.T01:CDS 45.0%
GAGACACTCTCCCTTATCAA+TGG + Chr5:93133381-93133400 MsG0580029167.01.T01:CDS 45.0%
GAGCATGCTGTCCAAAAAGA+TGG + Chr5:93134774-93134793 MsG0580029167.01.T01:intron 45.0%
GAGGTTCATGAAGACGAACA+AGG + Chr5:93133528-93133547 MsG0580029167.01.T01:CDS 45.0%
GATCAGCTAAGAGATCAACG+TGG - Chr5:93133437-93133456 None:intergenic 45.0%
GGGACGGTAACCATTGATAA+GGG - Chr5:93133394-93133413 None:intergenic 45.0%
GTTCATGAAGACGAACAAGG+TGG + Chr5:93133531-93133550 MsG0580029167.01.T01:CDS 45.0%
TCGAAAAATCTGGCAGTGCT+TGG + Chr5:93133266-93133285 MsG0580029167.01.T01:CDS 45.0%
TCTCGTATCAATGGAGAGGA+TGG + Chr5:93135721-93135740 MsG0580029167.01.T01:CDS 45.0%
TGAGTCTCGTATCAATGGAG+AGG + Chr5:93135717-93135736 MsG0580029167.01.T01:CDS 45.0%
TGCCAAGGACTTACTTGACT+TGG - Chr5:93135352-93135371 None:intergenic 45.0%
TGGGACGGTAACCATTGATA+AGG - Chr5:93133395-93133414 None:intergenic 45.0%
TTTCGCGCATTCAGAAGGTA+CGG - Chr5:93133622-93133641 None:intergenic 45.0%
! ATTATCCGACGGCTAGTCAT+TGG + Chr5:93133976-93133995 MsG0580029167.01.T01:CDS 45.0%
! CCCGTGTTGATCGAAAAATC+TGG + Chr5:93133256-93133275 MsG0580029167.01.T01:CDS 45.0%
! TTACACCAATGACTAGCCGT+CGG - Chr5:93133984-93134003 None:intergenic 45.0%
! TTTTGTGGGGGGAATTGTAG+AGG - Chr5:93135406-93135425 None:intergenic 45.0%
!! GAACTCTATCTCTGGAATGG+AGG - Chr5:93135479-93135498 None:intergenic 45.0%
!! GATTTTTCGATCAACACGGG+TGG - Chr5:93133256-93133275 None:intergenic 45.0%
!!! GGTGATGGGCTTGTTTTTTC+TGG + Chr5:93133552-93133571 MsG0580029167.01.T01:CDS 45.0%
!! AAAATTGAAAAAATAATATA+AGG - Chr5:93135236-93135255 None:intergenic 5.0%
!! TTTATTAGATATTATTATTA+AGG + Chr5:93134532-93134551 MsG0580029167.01.T01:intron 5.0%
!!! TTTAAAATGTTTAAATTTAT+TGG + Chr5:93134457-93134476 MsG0580029167.01.T01:intron 5.0%
AAAATCTGGCAGTGCTTGGC+CGG + Chr5:93133270-93133289 MsG0580029167.01.T01:CDS 50.0%
AATGCCGTCTCAGGTACTTC+CGG + Chr5:93133674-93133693 MsG0580029167.01.T01:CDS 50.0%
ACAATCCCAAATGCCGTCTC+AGG + Chr5:93133665-93133684 MsG0580029167.01.T01:CDS 50.0%
AGATCTTCATCGGAATCCGC+AGG + Chr5:93133862-93133881 MsG0580029167.01.T01:CDS 50.0%
ATGCGCCATGGATGATGAGA+AGG - Chr5:93134149-93134168 None:intergenic 50.0%
CCGTCTGATGCTAATAACGG+TGG + Chr5:93133588-93133607 MsG0580029167.01.T01:CDS 50.0%
CGTTATGGAGGCAGCAGAAT+TGG + Chr5:93133923-93133942 MsG0580029167.01.T01:CDS 50.0%
GAAGACGAACAAGGTGGTGA+TGG + Chr5:93133537-93133556 MsG0580029167.01.T01:CDS 50.0%
GCATGTTCCAAGTGACCTAG+TGG - Chr5:93133342-93133361 None:intergenic 50.0%
GGGGGAAGCGATAAAGCTAT+CGG - Chr5:93133217-93133236 None:intergenic 50.0%
TATGGAGGCAGCAGAATTGG+CGG + Chr5:93133926-93133945 MsG0580029167.01.T01:CDS 50.0%
TCGTCTTCATGAACCTCCTG+AGG - Chr5:93133525-93133544 None:intergenic 50.0%
TGGGATTGTAGGTCAAGTGG+CGG - Chr5:93133654-93133673 None:intergenic 50.0%
! GAAGTACCTGAGACGGCATT+TGG - Chr5:93133674-93133693 None:intergenic 50.0%
! GAGACGGCATTTGGGATTGT+AGG - Chr5:93133665-93133684 None:intergenic 50.0%
! GTGGGTTTCTGAGGATCCAT+GGG - Chr5:93133237-93133256 None:intergenic 50.0%
! TGGGTTTCTGAGGATCCATG+GGG - Chr5:93133236-93133255 None:intergenic 50.0%
! TGGTTTTCACGAAGCTCCTC+AGG + Chr5:93133506-93133525 MsG0580029167.01.T01:CDS 50.0%
! TTTTCACGAAGCTCCTCAGG+AGG + Chr5:93133509-93133528 MsG0580029167.01.T01:CDS 50.0%
!! AGTGGTGATTAGGTGTCGCT+TGG - Chr5:93133754-93133773 None:intergenic 50.0%
!! CAGCACTTAACTTGTGCCTG+CGG - Chr5:93133881-93133900 None:intergenic 50.0%
!! CGGAATTTTGACCGATGGTC+CGG - Chr5:93133292-93133311 None:intergenic 50.0%
!! GTGGTGATTAGGTGTCGCTT+GGG - Chr5:93133753-93133772 None:intergenic 50.0%
!! TAGCTTTATCGCTTCCCCCA+TGG + Chr5:93133218-93133237 MsG0580029167.01.T01:CDS 50.0%
!! TTGACCGGAAGTACCTGAGA+CGG - Chr5:93133681-93133700 None:intergenic 50.0%
AACTTTCGCCACACCTACCG+TGG + Chr5:93133723-93133742 MsG0580029167.01.T01:CDS 55.0%
ACTTTCGCCACACCTACCGT+GGG + Chr5:93133724-93133743 MsG0580029167.01.T01:CDS 55.0%
CATACCTGAAGCATGCGCCA+TGG - Chr5:93134161-93134180 None:intergenic 55.0%
GAGACGACGCAGGGAATGAT+GGG - Chr5:93133414-93133433 None:intergenic 55.0%
GGGAATTGTAGAGGCAGTGC+TGG - Chr5:93135397-93135416 None:intergenic 55.0%
GGGATTGTAGGTCAAGTGGC+GGG - Chr5:93133653-93133672 None:intergenic 55.0%
GTCTCAGGTACTTCCGGTCA+AGG + Chr5:93133680-93133699 MsG0580029167.01.T01:CDS 55.0%
TCATCCATGGCGCATGCTTC+AGG + Chr5:93134154-93134173 MsG0580029167.01.T01:CDS 55.0%
! GGCAGCAGAATTGGCGGATA+AGG + Chr5:93133932-93133951 MsG0580029167.01.T01:CDS 55.0%
! GGGTTTCTGAGGATCCATGG+GGG - Chr5:93133235-93133254 None:intergenic 55.0%
! GGTGGGTTTCTGAGGATCCA+TGG - Chr5:93133238-93133257 None:intergenic 55.0%
! TAGAGGCAGTGCTGGAGTGT+GGG - Chr5:93135389-93135408 None:intergenic 55.0%
! TCAACACGGGTGGGTTTCTG+AGG - Chr5:93133246-93133265 None:intergenic 55.0%
!! AGGGAGAGTGTCTCGGTGTT+AGG - Chr5:93133375-93133394 None:intergenic 55.0%
!! AGTGTCTCGGTGTTAGGGGA+AGG - Chr5:93133369-93133388 None:intergenic 55.0%
!! GGAGAGTGTCTCGGTGTTAG+GGG - Chr5:93133373-93133392 None:intergenic 55.0%
!! GGGAGAGTGTCTCGGTGTTA+GGG - Chr5:93133374-93133393 None:intergenic 55.0%
!! GTGTCTCGGTGTTAGGGGAA+GGG - Chr5:93133368-93133387 None:intergenic 55.0%
ATCAACGTGGGAGACGACGC+AGG - Chr5:93133424-93133443 None:intergenic 60.0%
GGAGACGACGCAGGGAATGA+TGG - Chr5:93133415-93133434 None:intergenic 60.0%
TCAACGTGGGAGACGACGCA+GGG - Chr5:93133423-93133442 None:intergenic 60.0%
! CGACGCAGGGAATGATGGGA+CGG - Chr5:93133410-93133429 None:intergenic 60.0%
! GTAGAGGCAGTGCTGGAGTG+TGG - Chr5:93135390-93135409 None:intergenic 60.0%
GCAGTGCTTGGCCGGACCAT+CGG + Chr5:93133278-93133297 MsG0580029167.01.T01:CDS 65.0%
TCGCTTGGGAGACCCACGGT+AGG - Chr5:93133739-93133758 None:intergenic 65.0%
TGGGAGACCCACGGTAGGTG+TGG - Chr5:93133734-93133753 None:intergenic 65.0%
!! GGTGTCGCTTGGGAGACCCA+CGG - Chr5:93133743-93133762 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 93133159 93135753 93133159 ID=MsG0580029167.01;Name=MsG0580029167.01
Chr5 mRNA 93133159 93135753 93133159 ID=MsG0580029167.01.T01;Parent=MsG0580029167.01;Name=MsG0580029167.01.T01;_AED=0.19;_eAED=0.19;_QI=0|0|0|1|1|1|2|0|484
Chr5 exon 93133159 93134175 93133159 ID=MsG0580029167.01.T01:exon:17961;Parent=MsG0580029167.01.T01
Chr5 exon 93135316 93135753 93135316 ID=MsG0580029167.01.T01:exon:17962;Parent=MsG0580029167.01.T01
Chr5 CDS 93133159 93134175 93133159 ID=MsG0580029167.01.T01:cds;Parent=MsG0580029167.01.T01
Chr5 CDS 93135316 93135753 93135316 ID=MsG0580029167.01.T01:cds;Parent=MsG0580029167.01.T01
Gene Sequence

>MsG0580029167.01.T01

TTGAAAAACCCTAATCATATTTTCTGGCGCTCTCTAACAAATTCTTTCTTCTCTTCCGATAGCTTTATCGCTTCCCCCATGGATCCTCAGAAACCCACCCGTGTTGATCGAAAAATCTGGCAGTGCTTGGCCGGACCATCGGTCAAAATTCCGAAACTAAATTCTCAAGTTTTTTACTTTCCACTAGGTCACTTGGAACATGCATGCCCTTCCCCTAACACCGAGACACTCTCCCTTATCAATGGTTACCGTCCCATCATTCCCTGCGTCGTCTCCCACGTTGATCTCTTAGCTGATCTTCAAACCGATGAAGTCTTTGCAAAACTGCTTCTAACTCCCATCACCAATGGTTTTCACGAAGCTCCTCAGGAGGTTCATGAAGACGAACAAGGTGGTGATGGGCTTGTTTTTTCTGGCAAGACTCTCACCCCGTCTGATGCTAATAACGGTGGTGCATTCTCCGTACCTTCTGAATGCGCGAAATTGATATTCCCGCCACTTGACCTACAATCCCAAATGCCGTCTCAGGTACTTCCGGTCAAGGACATCCACAATTTTGTTTGGAACTTTCGCCACACCTACCGTGGGTCTCCCAAGCGACACCTAATCACCACTAACTGGAGTAAGTTCGTTGACACAGAGAAAATTATTGGCGGCGATTCCCTCGTTTTAATAAAAAATTCTAAAGATAAAGATAAAGATGAGATCTTCATCGGAATCCGCAGGCACAAGTTAAGTGCTGCTGCTAAGATAACGGAAAAATCCGTTATGGAGGCAGCAGAATTGGCGGATAAGGACATGACTTTTGAAGTTATTTATTATCCGACGGCTAGTCATTGGTGTAATTTTGTGGTGGAAGCTGAAGTTGTAAAGAAAGCAATGCAAATTAATTGGCAATCTGGAATGAGAGTTAAACTTTGTTTGAAGACTGATGAATCTTCAAAGAGAAGCTCAATTTTTCAAGGGACAGTATCTGCTTTATCTGATCCTTCTCATCATCCATGGCGCATGCTTCAGGTTAACTGGGATGAATCTGAAGTCTCTCAAAATCCAAGTCAAGTAAGTCCTTGGCAGATTGAACTTATTTCCCACACTCCAGCACTGCCTCTACAATTCCCCCCACAAAAAAAGCTTAGAATTGCTCAGGGTTCTGCCTTATCAACTAACATAGAGAGACCCTCCATTCCAGAGATAGAGTTCAATTTCTTAAACCCATCGTCGTTGAATTATGATGCTTTTCTTGATAGCATGAAGGGAACCATACCTAATCTTTTTTCTGCCTCAACTTCTTCCAACTCTTTGAATGATAATGACAATGGTTTTGTTGAAGGTACCCACAACCTCAACACCAAAAAAGTTAATCATGGTTCTATTAAGCTATTTGGTAAGACCATAGAGGTTGTTGAAAAGGATTTGCATGAGTCTCGTATCAATGGAGAGGATGGTTCGGAATAA
Protein sequence

>MsG0580029167.01.T01

LKNPNHIFWRSLTNSFFSSDSFIASPMDPQKPTRVDRKIWQCLAGPSVKIPKLNSQVFYFPLGHLEHACPSPNTETLSLINGYRPIIPCVVSHVDLLADLQTDEVFAKLLLTPITNGFHEAPQEVHEDEQGGDGLVFSGKTLTPSDANNGGAFSVPSECAKLIFPPLDLQSQMPSQVLPVKDIHNFVWNFRHTYRGSPKRHLITTNWSKFVDTEKIIGGDSLVLIKNSKDKDKDEIFIGIRRHKLSAAAKITEKSVMEAAELADKDMTFEVIYYPTASHWCNFVVEAEVVKKAMQINWQSGMRVKLCLKTDESSKRSSIFQGTVSALSDPSHHPWRMLQVNWDESEVSQNPSQVSPWQIELISHTPALPLQFPPQKKLRIAQGSALSTNIERPSIPEIEFNFLNPSSLNYDAFLDSMKGTIPNLFSASTSSNSLNDNDNGFVEGTHNLNTKKVNHGSIKLFGKTIEVVEKDLHESRINGEDGSE*