AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029249.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029249.01.T01 MTR_5g083230 98.204 167 2 1 1 166 1 167 5.01e-122 340
MsG0580029249.01.T01 MTR_3g071590 73.171 164 35 4 1 160 1 159 2.03e-80 235
MsG0580029249.01.T01 MTR_3g031660 65.909 176 44 5 1 160 1 176 9.92e-73 216
MsG0580029249.01.T01 MTR_4g083680 56.098 164 61 1 2 165 11 163 1.63e-58 180
MsG0580029249.01.T01 MTR_2g068760 76.364 110 26 0 2 111 9 118 1.91e-58 179
MsG0580029249.01.T01 MTR_2g093310 62.832 113 42 0 5 117 32 144 9.34e-51 161
MsG0580029249.01.T01 MTR_7g033800 53.020 149 66 1 4 148 9 157 2.03e-50 160
MsG0580029249.01.T01 MTR_4g105170 52.532 158 58 1 4 144 9 166 2.24e-50 160
MsG0580029249.01.T01 MTR_3g077240 71.154 104 30 0 4 107 8 111 4.31e-50 159
MsG0580029249.01.T01 MTR_4g060950 64.035 114 40 1 5 117 23 136 1.79e-49 158
MsG0580029249.01.T01 MTR_5g083960 60.504 119 47 0 1 119 34 152 1.07e-48 156
MsG0580029249.01.T01 MTR_6g005070 57.377 122 52 0 4 125 7 128 1.56e-48 154
MsG0580029249.01.T01 MTR_1g095850 64.865 111 39 0 7 117 73 183 5.51e-48 155
MsG0580029249.01.T01 MTR_3g094690 69.903 103 31 0 5 107 46 148 1.31e-47 154
MsG0580029249.01.T01 MTR_6g005080 49.315 146 63 1 4 149 8 142 2.56e-47 150
MsG0580029249.01.T01 MTR_3g073690 61.207 116 44 1 5 120 8 122 1.99e-46 150
MsG0580029249.01.T01 MTR_3g094690 68.932 103 31 1 5 107 46 147 5.01e-45 148
MsG0580029249.01.T01 MTR_5g017950 59.200 125 50 1 4 127 6 130 6.41e-45 150
MsG0580029249.01.T01 MTR_3g071420 67.000 100 33 0 4 103 12 111 6.30e-44 144
MsG0580029249.01.T01 MTR_3g452660 62.136 103 39 0 4 106 5 107 6.97e-44 147
MsG0580029249.01.T01 MTR_5g075020 68.932 103 32 0 5 107 42 144 2.27e-43 144
MsG0580029249.01.T01 MTR_5g075020 68.932 103 32 0 5 107 46 148 4.07e-43 143
MsG0580029249.01.T01 MTR_5g083010 63.366 101 37 0 3 103 8 108 6.23e-42 138
MsG0580029249.01.T01 MTR_8g040900 63.551 107 39 0 4 110 5 111 1.67e-41 139
MsG0580029249.01.T01 MTR_5g093010 52.294 109 52 0 7 115 6 114 9.67e-39 129
MsG0580029249.01.T01 MTR_7g096530 56.604 106 45 1 2 106 10 115 1.93e-38 130
MsG0580029249.01.T01 MTR_1g070220 59.406 101 40 1 6 105 15 115 1.10e-37 128
MsG0580029249.01.T01 MTR_8g036105 52.846 123 54 2 3 121 15 137 1.64e-37 128
MsG0580029249.01.T01 MTR_7g028905 50.450 111 55 0 5 115 4 114 4.80e-37 124
MsG0580029249.01.T01 MTR_7g075230 45.455 165 78 5 3 164 9 164 9.45e-37 125
MsG0580029249.01.T01 MTR_8g036085 55.882 102 44 1 6 106 23 124 8.32e-36 124
MsG0580029249.01.T01 MTR_7g074990 57.843 102 42 1 6 106 20 121 9.44e-36 124
MsG0580029249.01.T01 MTR_7g096610 47.143 140 62 4 2 130 4 142 1.21e-34 121
MsG0580029249.01.T01 MTR_5g080470 42.177 147 72 3 2 140 3 144 2.61e-33 117
MsG0580029249.01.T01 MTR_1g070205 56.701 97 39 2 6 100 12 107 6.06e-33 117
MsG0580029249.01.T01 MTR_6g027710 49.038 104 52 1 5 107 39 142 4.68e-32 114
MsG0580029249.01.T01 MTR_8g079660 48.515 101 52 0 6 106 8 108 1.67e-30 111
MsG0580029249.01.T01 MTR_8g079580 48.544 103 52 1 6 107 8 110 6.96e-29 106
MsG0580029249.01.T01 MTR_8g079620 45.545 101 55 0 6 106 8 108 1.60e-28 105
MsG0580029249.01.T01 MTR_6g027700 46.535 101 53 1 6 105 7 107 2.04e-28 104
MsG0580029249.01.T01 MTR_6g011250 40.351 114 67 1 1 113 4 117 4.21e-27 100
MsG0580029249.01.T01 MTR_4g107450 43.220 118 66 1 2 118 6 123 2.62e-24 96.3
MsG0580029249.01.T01 MTR_6g011230 41.935 93 54 0 7 99 12 104 3.07e-24 94.0
MsG0580029249.01.T01 MTR_6g011200 44.578 83 45 1 4 85 7 89 5.11e-23 92.0
MsG0580029249.01.T01 MTR_2g100020 35.238 105 68 0 2 106 15 119 5.02e-21 87.0
MsG0580029249.01.T01 MTR_4g099230 40.426 94 51 2 7 95 3 96 9.34e-21 83.6
MsG0580029249.01.T01 MTR_8g017090 31.159 138 95 0 7 144 13 150 9.56e-20 84.0
MsG0580029249.01.T01 MTR_4g088035 39.080 87 53 0 7 93 7 93 3.07e-19 82.0
MsG0580029249.01.T01 MTR_5g085390 39.759 83 49 1 4 85 7 89 6.95e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029249.01.T01 AT2G30130 78.947 133 24 1 3 131 5 137 8.76e-74 219
MsG0580029249.01.T01 AT1G31320 70.940 117 34 0 2 118 9 125 3.26e-52 164
MsG0580029249.01.T01 AT2G28500 55.714 140 61 1 5 144 54 192 7.97e-52 165
MsG0580029249.01.T01 AT1G07900 59.091 132 53 1 5 136 32 162 2.67e-51 162
MsG0580029249.01.T01 AT1G16530 70.093 107 31 1 5 110 13 119 1.52e-50 160
MsG0580029249.01.T01 AT5G63090 70.874 103 30 0 4 106 9 111 1.42e-49 158
MsG0580029249.01.T01 AT5G63090 70.874 103 30 0 4 106 9 111 1.42e-49 158
MsG0580029249.01.T01 AT5G63090 70.874 103 30 0 4 106 9 111 1.42e-49 158
MsG0580029249.01.T01 AT5G63090 70.874 103 30 0 4 106 9 111 1.42e-49 158
MsG0580029249.01.T01 AT3G27650 67.308 104 34 0 4 107 37 140 1.17e-48 155
MsG0580029249.01.T01 AT2G30340 60.504 119 47 0 5 123 51 169 3.91e-47 154
MsG0580029249.01.T01 AT2G30340 60.504 119 47 0 5 123 52 170 4.77e-47 154
MsG0580029249.01.T01 AT2G23660 57.265 117 49 1 4 120 3 118 1.94e-45 151
MsG0580029249.01.T01 AT2G23660 57.265 117 49 1 4 120 3 118 1.94e-45 151
MsG0580029249.01.T01 AT2G23660 57.265 117 49 1 4 120 3 118 1.94e-45 151
MsG0580029249.01.T01 AT2G40470 70.874 103 30 0 5 107 19 121 3.64e-45 147
MsG0580029249.01.T01 AT2G40470 70.874 103 30 0 5 107 44 146 5.55e-45 147
MsG0580029249.01.T01 AT5G66870 64.078 103 37 0 4 106 5 107 7.05e-44 147
MsG0580029249.01.T01 AT3G26660 59.091 110 45 0 7 116 6 115 1.61e-43 140
MsG0580029249.01.T01 AT3G26620 59.091 110 45 0 7 116 6 115 2.05e-43 140
MsG0580029249.01.T01 AT3G11090 58.621 116 47 1 4 118 9 124 1.26e-41 137
MsG0580029249.01.T01 AT1G65620 66.019 103 35 0 4 106 7 109 3.80e-41 137
MsG0580029249.01.T01 AT1G65620 66.019 103 35 0 4 106 7 109 3.80e-41 137
MsG0580029249.01.T01 AT1G65620 66.019 103 35 0 4 106 7 109 3.80e-41 137
MsG0580029249.01.T01 AT1G65620 66.019 103 35 0 4 106 7 109 3.80e-41 137
MsG0580029249.01.T01 AT1G65620 66.019 103 35 0 4 106 7 109 3.80e-41 137
MsG0580029249.01.T01 AT4G00210 45.283 159 70 3 2 159 7 149 2.28e-37 128
MsG0580029249.01.T01 AT2G42430 56.604 106 45 1 2 106 11 116 5.92e-37 127
MsG0580029249.01.T01 AT2G45410 50.388 129 60 2 9 133 19 147 1.91e-36 125
MsG0580029249.01.T01 AT3G03760 54.808 104 46 1 5 107 50 153 3.16e-36 126
MsG0580029249.01.T01 AT2G42440 47.143 140 64 4 2 139 3 134 4.81e-35 122
MsG0580029249.01.T01 AT2G31310 45.455 143 66 4 1 132 1 142 7.87e-35 120
MsG0580029249.01.T01 AT2G45420 52.885 104 48 1 9 111 40 143 7.25e-34 120
MsG0580029249.01.T01 AT4G00220 53.846 104 47 1 9 111 20 123 1.43e-33 118
MsG0580029249.01.T01 AT3G58190 48.031 127 58 3 6 130 11 131 2.66e-33 117
MsG0580029249.01.T01 AT5G06080 52.727 110 46 2 5 113 6 110 9.98e-32 112
MsG0580029249.01.T01 AT3G50510 47.573 103 54 0 4 106 10 112 2.74e-30 109
MsG0580029249.01.T01 AT3G50510 47.573 103 54 0 4 106 10 112 2.74e-30 109
MsG0580029249.01.T01 AT5G35900 42.718 103 59 0 7 109 6 108 2.03e-25 97.1
MsG0580029249.01.T01 AT1G72980 39.130 115 69 1 2 115 9 123 1.84e-23 92.4
MsG0580029249.01.T01 AT3G47870 36.190 105 67 0 2 106 32 136 3.85e-23 93.6
MsG0580029249.01.T01 AT3G13850 38.947 95 58 0 7 101 37 131 9.18e-22 89.0
MsG0580029249.01.T01 AT1G06280 45.455 77 42 0 7 83 25 101 2.01e-19 81.6
MsG0580029249.01.T01 AT2G19820 37.079 89 55 1 5 92 11 99 1.33e-16 72.0
MsG0580029249.01.T01 AT4G22700 36.634 101 62 2 7 106 6 105 8.90e-16 71.6
MsG0580029249.01.T01 AT5G15060 43.243 74 38 2 7 77 18 90 5.29e-14 65.9
MsG0580029249.01.T01 AT1G36000 29.348 92 65 0 1 92 4 95 8.19e-13 62.0
MsG0580029249.01.T01 AT2G19510 27.174 92 67 0 1 92 4 95 7.75e-11 57.0

Find 21 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCTGCTCTTTGGTGAAT+TGG 0.247897 5:-94266953 None:intergenic
CTCACTCAATACCTTAATTA+TGG 0.252400 5:+94267193 MsG0580029249.01.T01:CDS
TGTGAACTATGGCAAACTTT+TGG 0.274301 5:-94266108 None:intergenic
CATCTGCTCTTTGGTGAATT+GGG 0.326240 5:-94266952 None:intergenic
TAGAAGAAGAACCATAATTA+AGG 0.338211 5:-94267204 None:intergenic
TGTTCACTGCATCTGCTCTT+TGG 0.348086 5:-94266961 None:intergenic
TATAGTAAGATTTCTGATCT+TGG 0.371111 5:-94267159 None:intergenic
GCTCCAACACAGCCATAAAC+TGG 0.374722 5:-94267019 None:intergenic
TAATATGTATGGACATGATA+TGG 0.378652 5:+94267261 MsG0580029249.01.T01:CDS
AGAGTGAGGGATCCAGTTTA+TGG 0.403662 5:+94267007 MsG0580029249.01.T01:CDS
TCTTGGTTGATTTGTGGTGT+TGG 0.412347 5:-94267142 None:intergenic
GGTTCATGAGAAGGAAAATA+AGG 0.413213 5:-94266085 None:intergenic
CAATGTTAGCAAAATGTTGC+AGG 0.415314 5:+94266147 MsG0580029249.01.T01:CDS
CTTAAGAGAGATAATATGTA+TGG 0.442326 5:+94267250 MsG0580029249.01.T01:CDS
TTCTGATCTTGGTTGATTTG+TGG 0.443495 5:-94267148 None:intergenic
GATCCAGTTTATGGCTGTGT+TGG 0.485932 5:+94267016 MsG0580029249.01.T01:CDS
AAAGTTTGCCATAGTTCACA+AGG 0.602163 5:+94266111 MsG0580029249.01.T01:CDS
AGAAGGAAAATAAGGAGCAA+AGG 0.607624 5:-94266077 None:intergenic
ATGAAGCAAATGCAAGAGTG+AGG 0.619290 5:+94266993 MsG0580029249.01.T01:CDS
AGAAGCTTGCATGAAGCACA+TGG 0.628381 5:-94266028 None:intergenic
TGAAGCAAATGCAAGAGTGA+GGG 0.632506 5:+94266994 MsG0580029249.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATACTAATCATTAGAA+AGG - Chr5:94266643-94266662 None:intergenic 15.0%
!! AGTTTGTGAATAAAATATTT+GGG + Chr5:94266331-94266350 MsG0580029249.01.T01:intron 15.0%
!!! ATAAATTTTTTGTTTGAATC+AGG + Chr5:94266925-94266944 MsG0580029249.01.T01:intron 15.0%
!!! TAGTTTGTGAATAAAATATT+TGG + Chr5:94266330-94266349 MsG0580029249.01.T01:intron 15.0%
!!! TTTTTTATGTTTCTTACTTT+AGG - Chr5:94266431-94266450 None:intergenic 15.0%
!! AAACACTTAAAAAAAGTAAG+AGG - Chr5:94266175-94266194 None:intergenic 20.0%
!! TAAGTGTTTATTATTGTTCA+AGG + Chr5:94266685-94266704 MsG0580029249.01.T01:intron 20.0%
!! TAATAGTAAACAGCTAAAAA+AGG - Chr5:94266489-94266508 None:intergenic 20.0%
!! TGTTTACTATTATAGTTATG+AGG + Chr5:94266497-94266516 MsG0580029249.01.T01:intron 20.0%
!!! TACTTTCTTTGATGATTAAA+GGG + Chr5:94266199-94266218 MsG0580029249.01.T01:intron 20.0%
!!! TTACTTTCTTTGATGATTAA+AGG + Chr5:94266198-94266217 MsG0580029249.01.T01:intron 20.0%
!!! TTATCTTTTGTACTTATGTT+TGG + Chr5:94266289-94266308 MsG0580029249.01.T01:intron 20.0%
! ACAAAAAACACAATCAAATG+TGG - Chr5:94266574-94266593 None:intergenic 25.0%
! CTTAAGAGAGATAATATGTA+TGG + Chr5:94267250-94267269 MsG0580029249.01.T01:CDS 25.0%
! TAATATGTATGGACATGATA+TGG + Chr5:94267261-94267280 MsG0580029249.01.T01:CDS 25.0%
! TAGAAGAAGAACCATAATTA+AGG - Chr5:94267207-94267226 None:intergenic 25.0%
! TATAGTAAGATTTCTGATCT+TGG - Chr5:94267162-94267181 None:intergenic 25.0%
!!! TTCTTACTTTAGGATGTTTT+AGG - Chr5:94266421-94266440 None:intergenic 25.0%
!!! TTTTTTTATGCGGACAATTA+GGG - Chr5:94266763-94266782 None:intergenic 25.0%
!!! TTTTTTTTATGCGGACAATT+AGG - Chr5:94266764-94266783 None:intergenic 25.0%
AAGAACACAACAATTGATCA+CGG - Chr5:94266828-94266847 None:intergenic 30.0%
ATTATAGTTATGAGGAGTTG+AGG + Chr5:94266505-94266524 MsG0580029249.01.T01:intron 30.0%
CTCACTCAATACCTTAATTA+TGG + Chr5:94267193-94267212 MsG0580029249.01.T01:CDS 30.0%
CTCTATTGTTGAAGACATAT+TGG + Chr5:94266795-94266814 MsG0580029249.01.T01:intron 30.0%
!! GTAGAATCAGACTTGTTATT+CGG + Chr5:94266866-94266885 MsG0580029249.01.T01:intron 30.0%
AAAGTTTGCCATAGTTCACA+AGG + Chr5:94266111-94266130 MsG0580029249.01.T01:CDS 35.0%
ACCAAAAACCTTGTGAACTA+TGG - Chr5:94266122-94266141 None:intergenic 35.0%
AGAAGGAAAATAAGGAGCAA+AGG - Chr5:94266080-94266099 None:intergenic 35.0%
CAATGTTAGCAAAATGTTGC+AGG + Chr5:94266147-94266166 MsG0580029249.01.T01:CDS 35.0%
CACAAACTATTCAACCTACA+AGG - Chr5:94266319-94266338 None:intergenic 35.0%
GGTTCATGAGAAGGAAAATA+AGG - Chr5:94266088-94266107 None:intergenic 35.0%
GTTAATGATTGCAATACGAG+AGG + Chr5:94266603-94266622 MsG0580029249.01.T01:intron 35.0%
! GTGAACTATGGCAAACTTTT+GGG - Chr5:94266110-94266129 None:intergenic 35.0%
! TGAACTATGGCAAACTTTTG+GGG - Chr5:94266109-94266128 None:intergenic 35.0%
! TGTGAACTATGGCAAACTTT+TGG - Chr5:94266111-94266130 None:intergenic 35.0%
! TTATGTTTGGTTCACCTTGT+AGG + Chr5:94266302-94266321 MsG0580029249.01.T01:intron 35.0%
!! TTCTGATCTTGGTTGATTTG+TGG - Chr5:94267151-94267170 None:intergenic 35.0%
ACAACAATTGATCACGGAGT+TGG - Chr5:94266822-94266841 None:intergenic 40.0%
ATAGTTATGAGGAGTTGAGG+AGG + Chr5:94266508-94266527 MsG0580029249.01.T01:intron 40.0%
ATGAAGCAAATGCAAGAGTG+AGG + Chr5:94266993-94267012 MsG0580029249.01.T01:CDS 40.0%
CAACAATTGATCACGGAGTT+GGG - Chr5:94266821-94266840 None:intergenic 40.0%
CATCTGCTCTTTGGTGAATT+GGG - Chr5:94266955-94266974 None:intergenic 40.0%
TGAAGCAAATGCAAGAGTGA+GGG + Chr5:94266994-94267013 MsG0580029249.01.T01:CDS 40.0%
! CATTTTGCATCTGGATGCAT+AGG - Chr5:94267111-94267130 None:intergenic 40.0%
! GCCATAGTTCACAAGGTTTT+TGG + Chr5:94266118-94266137 MsG0580029249.01.T01:CDS 40.0%
! GGGTTAGAGAGTACAAAACT+TGG - Chr5:94266743-94266762 None:intergenic 40.0%
! TCACTTGCTCATTTTGCATC+TGG - Chr5:94267120-94267139 None:intergenic 40.0%
!! TCTTGGTTGATTTGTGGTGT+TGG - Chr5:94267145-94267164 None:intergenic 40.0%
!!! AACTTTTGGGGTTCATGAGA+AGG - Chr5:94266097-94266116 None:intergenic 40.0%
AGAAGCTTGCATGAAGCACA+TGG - Chr5:94266031-94266050 None:intergenic 45.0%
AGAGTGAGGGATCCAGTTTA+TGG + Chr5:94267007-94267026 MsG0580029249.01.T01:CDS 45.0%
GATCCAGTTTATGGCTGTGT+TGG + Chr5:94267016-94267035 MsG0580029249.01.T01:CDS 45.0%
GCATCTGCTCTTTGGTGAAT+TGG - Chr5:94266956-94266975 None:intergenic 45.0%
TGTTCACTGCATCTGCTCTT+TGG - Chr5:94266964-94266983 None:intergenic 45.0%
TTGCGACACAATCACGATTG+AGG + Chr5:94266539-94266558 MsG0580029249.01.T01:intron 45.0%
!!! ATTTTTTTTTTTTTTTTATG+CGG - Chr5:94266773-94266792 None:intergenic 5.0%
GCTCCAACACAGCCATAAAC+TGG - Chr5:94267022-94267041 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 94266013 94267291 94266013 ID=MsG0580029249.01;Name=MsG0580029249.01
Chr5 mRNA 94266013 94267291 94266013 ID=MsG0580029249.01.T01;Parent=MsG0580029249.01;Name=MsG0580029249.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|166
Chr5 exon 94266013 94266168 94266013 ID=MsG0580029249.01.T01:exon:26719;Parent=MsG0580029249.01.T01
Chr5 exon 94266947 94267291 94266947 ID=MsG0580029249.01.T01:exon:26720;Parent=MsG0580029249.01.T01
Chr5 CDS 94266013 94266168 94266013 ID=MsG0580029249.01.T01:cds;Parent=MsG0580029249.01.T01
Chr5 CDS 94266947 94267291 94266947 ID=MsG0580029249.01.T01:cds;Parent=MsG0580029249.01.T01
Gene Sequence

>MsG0580029249.01.T01

ATGGGTGGAAATTCACCATGTGCTTCATGCAAGCTTCTGAGACGAAGATGCACAAAAGATTGCACCTTTGCTCCTTATTTTCCTTCTCATGAACCCCAAAAGTTTGCCATAGTTCACAAGGTTTTTGGTGCTAGCAATGTTAGCAAAATGTTGCAGGAGCTCCCAATTCACCAAAGAGCAGATGCAGTGAACAGTTTAGTCTATGAAGCAAATGCAAGAGTGAGGGATCCAGTTTATGGCTGTGTTGGAGCAATATCTTACTTGCAAAATCAAGTTTCTGAGCTTCAAATGCAACTTGCAGTTGCTCAAGCAGAGATCCTATGCATCCAGATGCAAAATGAGCAAGTGATTCCAACACCACAAATCAACCAAGATCAGAAATCTTACTATATTCAAGATGAACTCACTCAATACCTTAATTATGGTTCTTCTTCTAGTAATAATGTTATTCATGATTCTCTTAAGAGAGATAATATGTATGGACATGATATGGTTTCTTGA

Protein sequence

>MsG0580029249.01.T01

MGGNSPCASCKLLRRRCTKDCTFAPYFPSHEPQKFAIVHKVFGASNVSKMLQELPIHQRADAVNSLVYEANARVRDPVYGCVGAISYLQNQVSELQMQLAVAQAEILCIQMQNEQVIPTPQINQDQKSYYIQDELTQYLNYGSSSSNNVIHDSLKRDNMYGHDMVS*