Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029253.01.T01 | AES99655.2 | 96.873 | 1343 | 40 | 2 | 1 | 1342 | 1 | 1342 | 0 | 2708 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029253.01.T01 | MTR_5g083300 | 96.873 | 1343 | 40 | 2 | 1 | 1342 | 1 | 1342 | 0.0 | 2708 |
| MsG0580029253.01.T01 | MTR_5g083300 | 97.088 | 1305 | 37 | 1 | 39 | 1342 | 1 | 1305 | 0.0 | 2639 |
| MsG0580029253.01.T01 | MTR_3g071860 | 55.838 | 1336 | 479 | 26 | 42 | 1340 | 5 | 1266 | 0.0 | 1382 |
| MsG0580029253.01.T01 | MTR_7g026650 | 62.743 | 875 | 292 | 15 | 477 | 1341 | 303 | 1153 | 0.0 | 1073 |
| MsG0580029253.01.T01 | MTR_7g026650 | 46.178 | 314 | 140 | 7 | 57 | 358 | 11 | 307 | 6.77e-72 | 263 |
| MsG0580029253.01.T01 | MTR_1g081750 | 58.653 | 861 | 321 | 16 | 486 | 1340 | 284 | 1115 | 0.0 | 962 |
| MsG0580029253.01.T01 | MTR_1g081750 | 46.125 | 271 | 123 | 7 | 38 | 295 | 1 | 261 | 2.04e-58 | 221 |
| MsG0580029253.01.T01 | MTR_7g026620 | 45.492 | 721 | 241 | 18 | 528 | 1244 | 72 | 644 | 7.28e-177 | 539 |
| MsG0580029253.01.T01 | MTR_7g450860 | 34.450 | 746 | 398 | 20 | 622 | 1329 | 567 | 1259 | 4.41e-109 | 375 |
| MsG0580029253.01.T01 | MTR_7g450820 | 35.745 | 691 | 367 | 19 | 664 | 1321 | 567 | 1213 | 1.43e-108 | 372 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029253.01.T01 | AT5G20420 | 52.844 | 1336 | 517 | 23 | 41 | 1340 | 2 | 1260 | 0.0 | 1277 |
| MsG0580029253.01.T01 | AT3G42670 | 65.990 | 788 | 250 | 8 | 561 | 1340 | 477 | 1254 | 0.0 | 1038 |
| MsG0580029253.01.T01 | AT3G42670 | 42.006 | 319 | 169 | 6 | 39 | 357 | 1 | 303 | 3.47e-62 | 234 |
| MsG0580029253.01.T01 | AT3G42670 | 65.693 | 787 | 256 | 6 | 561 | 1340 | 477 | 1256 | 0.0 | 1038 |
| MsG0580029253.01.T01 | AT3G42670 | 42.006 | 319 | 169 | 6 | 39 | 357 | 1 | 303 | 3.28e-62 | 234 |
| MsG0580029253.01.T01 | AT3G42670 | 65.990 | 788 | 250 | 8 | 561 | 1340 | 477 | 1254 | 0.0 | 1038 |
| MsG0580029253.01.T01 | AT3G42670 | 42.006 | 319 | 169 | 6 | 39 | 357 | 1 | 303 | 3.47e-62 | 234 |
| MsG0580029253.01.T01 | AT3G42670 | 65.990 | 788 | 250 | 8 | 561 | 1340 | 477 | 1254 | 0.0 | 1038 |
| MsG0580029253.01.T01 | AT3G42670 | 42.006 | 319 | 169 | 6 | 39 | 357 | 1 | 303 | 3.47e-62 | 234 |
| MsG0580029253.01.T01 | AT3G24340 | 32.192 | 730 | 426 | 17 | 622 | 1339 | 453 | 1125 | 4.98e-98 | 340 |
Find 334 sgRNAs with CRISPR-Local
Find 508 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGATTCTGACATGTT+TGG | 0.505618 | 5:-94305715 | MsG0580029253.01.T01:three_prime_UTR |
| AAACAATGATGAACAGAAAG+AGG | 0.499384 | 5:-94311801 | MsG0580029253.01.T01:CDS |
| AAACGGAACAAGAATGATAA+CGG | 0.591966 | 5:+94310135 | None:intergenic |
| AAACGGTGTTGGACAAAATG+TGG | 0.570352 | 5:-94309437 | MsG0580029253.01.T01:CDS |
| AAATACAAGAGGAAAAGGAA+AGG | 0.649041 | 5:-94307848 | MsG0580029253.01.T01:CDS |
| AAATGATCCGGCCGGTCCAA+GGG | 0.540267 | 5:-94312256 | MsG0580029253.01.T01:five_prime_UTR |
| AAATTATGAATAAAATGCTC+TGG | 0.541536 | 5:+94312394 | None:intergenic |
| AAATTCAGTGTGGCGCCAAG+AGG | 0.625626 | 5:-94308734 | MsG0580029253.01.T01:CDS |
| AACACTGCCTTTGATAATCT+AGG | 0.528465 | 5:-94312699 | MsG0580029253.01.T01:five_prime_UTR |
| AACACTTCGTGAGGGAATTT+CGG | 0.305259 | 5:+94307887 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAATGATGAACAGAAAG+AGG | - | Chr5:94307105-94307124 | MsG0580029253.01.T01:CDS | 30.0% | |
| AAACGGAACAAGAATGATAA+CGG | + | Chr5:94308774-94308793 | None:intergenic | 30.0% | |
| AAATACAAGAGGAAAAGGAA+AGG | - | Chr5:94311058-94311077 | MsG0580029253.01.T01:CDS | 30.0% | |
| AAGATCAGTAGAAAACAAGA+TGG | - | Chr5:94308407-94308426 | MsG0580029253.01.T01:CDS | 30.0% | |
| AAGGAACTAAGGAAAAACAA+TGG | + | Chr5:94309616-94309635 | None:intergenic | 30.0% | |
| AAGTTGCTCATATACAACAT+TGG | - | Chr5:94307075-94307094 | MsG0580029253.01.T01:CDS | 30.0% | |
| AATTCAAATGTACACATCTG+GGG | + | Chr5:94312697-94312716 | None:intergenic | 30.0% | |
| AATTGAGCCTAGATTATCAA+AGG | + | Chr5:94306217-94306236 | None:intergenic | 30.0% | |
| AGAAATGAGAACTCTTTGTA+AGG | - | Chr5:94308185-94308204 | MsG0580029253.01.T01:CDS | 30.0% | |
| AGAATGTGATTGTATGATCA+TGG | + | Chr5:94307549-94307568 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 94305706 | 94313222 | 94305706 | ID=MsG0580029253.01;Name=MsG0580029253.01 |
| Chr5 | mRNA | 94305706 | 94313222 | 94305706 | ID=MsG0580029253.01.T01;Parent=MsG0580029253.01;Name=MsG0580029253.01.T01;_AED=0.18;_eAED=0.18;_QI=1293|1|1|1|0.8|0.66|6|386|1342 |
| Chr5 | exon | 94305706 | 94306088 | 94305706 | ID=MsG0580029253.01.T01:exon:26747;Parent=MsG0580029253.01.T01 |
| Chr5 | exon | 94306815 | 94308761 | 94306815 | ID=MsG0580029253.01.T01:exon:26746;Parent=MsG0580029253.01.T01 |
| Chr5 | exon | 94309094 | 94309240 | 94309094 | ID=MsG0580029253.01.T01:exon:26745;Parent=MsG0580029253.01.T01 |
| Chr5 | exon | 94309330 | 94310850 | 94309330 | ID=MsG0580029253.01.T01:exon:26744;Parent=MsG0580029253.01.T01 |
| Chr5 | exon | 94311052 | 94311302 | 94311052 | ID=MsG0580029253.01.T01:exon:26743;Parent=MsG0580029253.01.T01 |
| Chr5 | exon | 94311764 | 94313222 | 94311764 | ID=MsG0580029253.01.T01:exon:26742;Parent=MsG0580029253.01.T01 |
| Chr5 | five_prime_UTR | 94311930 | 94313222 | 94311930 | ID=MsG0580029253.01.T01:five_prime_utr;Parent=MsG0580029253.01.T01 |
| Chr5 | CDS | 94311764 | 94311929 | 94311764 | ID=MsG0580029253.01.T01:cds;Parent=MsG0580029253.01.T01 |
| Chr5 | CDS | 94311052 | 94311302 | 94311052 | ID=MsG0580029253.01.T01:cds;Parent=MsG0580029253.01.T01 |
| Chr5 | CDS | 94309330 | 94310850 | 94309330 | ID=MsG0580029253.01.T01:cds;Parent=MsG0580029253.01.T01 |
| Chr5 | CDS | 94309094 | 94309240 | 94309094 | ID=MsG0580029253.01.T01:cds;Parent=MsG0580029253.01.T01 |
| Chr5 | CDS | 94306818 | 94308761 | 94306818 | ID=MsG0580029253.01.T01:cds;Parent=MsG0580029253.01.T01 |
| Chr5 | three_prime_UTR | 94306815 | 94306817 | 94306815 | ID=MsG0580029253.01.T01:three_prime_utr;Parent=MsG0580029253.01.T01 |
| Chr5 | three_prime_UTR | 94305706 | 94306088 | 94305706 | ID=MsG0580029253.01.T01:three_prime_utr;Parent=MsG0580029253.01.T01 |
| Gene Sequence |
>MsG0580029253.01.T01 ATGATTGATAAGCAATTTATGTGTGCTGATGAAGCCTTGACTGTAAGTGGAAATTTCTGTGCCAAATCAGCTGCTGAAGTTGCTCATATACAACATTGGCAATCACAAACAATGATGAACAGAAAGAGGCGTCTAAATGAATCAAAGAATTCATTCAATTCTGACCCATTTGAAGCAGTTATCTTTGGCTCATGGCATCCTGTGGAGTTCATAAAAGTTAAATCAGGATCTATGAGCATACATTTCACAGATAATCACCATATGGTTATGGACAAAGGTGCTTTATCAGACATTCGTATAAGGTCAAGGAAAGCTACTGTGTCAGATTGCTCCTGTTTCTTACGTCCAGGAATCGACATATGTGTGCTATCACCTCCTCAACGAGCCGGTGATTCAGTTGGTTTAAATCTCGAGCCTGTTTGGGCTGATGCTAGAATAAGTTCTATACAGAGAAAGCCCCATGGCTCAGAGTGCTCGTGTCAGTTTTTCGTCAACTTCTATATTGAACAAGGTTCACTTGGTTTAGAAATGAGAACTCTTTGTAAGGATGTCAAAGTATTCGGACTCAATCAGATCTCCATCCTCCAAAAAATCGAACACAGTCCATGTGAAAATCAGCCCCATCGGTGGTCTTCATCCGAGGATTGCTCTTCATTACCAAACACCAAATTGCTTTTGGGAAAGTTCTTAATCGATCTTTCATGGTTAGTTGTTACATCGGTTGTCAAGAATGTTTCGTTCTGTGCAAGATCAGTAGAAAACAAGATGGTGTATCAAATTTTGGGAGGTGATAGTAGTAATAGCTCATCGTCGAACACCGAGTCTCATATTGATGTCATCGGATTCAAAACCGACGATGGCATGTTAGTACCGATTGTTTCTCAAGTTGCTATAACTAACACCAAGAGAGCTGATCATGCACGTGAATCGCGTGCAGATGAAGCGTCATCATCTTATAATGTTGACGGCTTACGACGTTCCAAACGCAGGCATGTACAGCCAGAGCGTTACGTTGGCTGCGAAGTTAAAGAGTTGGATGTTGGTACCTTTAGAAACATGCCACCTGTCAGGATAGAAACATCCAAAGCAGTAGTAGATGAAATGTCGTTGCCGTTATCATTCTTGTTCCGTTTGCCGCAGAGCAGTCCGGAAAAGGGTGCCATCAAATGTCAGAAGGCCAGCAAACCGAATGCTTGTCGGGAGCTTCTGGTGTACAATAGGAGGGCTAAAACCCAAAAGGGGAAGAAGACTTGTGGCGGTGTTGATCAAAAGGAACATAAAAATCCACTTGCTATTATTCCTCTTCCTGATCAAGATGCTGATCCAATAGCCGTTGAGCATTACGATCCAAACGGCAACGTTGCTAGAAGTCATGAACATCAATCACGTGATATTACTTCGCAGTATAGTCATTTGGTTAATAATCCTAAACCAAGGAAAAACATCAATTTGTTAGATGATCCTGCAAAAGCAGATGATGCTGAGAAGAACGACCATGTTTCTTCACGATGTCAATTTTTTGGCTCTACTAAGTTACAAAGGAAGAGCACAGGTAGTTTGGATGATATGGATCTTGGAAATAGATGGGAAGGAATAAAAAGAAAATCGAAGACAGGATTTCATGAGGGAACATATCGCTCAACTCATTTAAGAAATAACGGCGAAGGAAGAAGTCACAATTATAAAGACAGAACTTTAAATGCGGCCGCATACAAGAGTTTGATCCATTCGTATTTACAGAATATTAATACAATACCTGTCATAGAAGAACCTCCTATCACTGACCAGTGGAAGAAATGTAACACAACAAACGGTGTTGGACAAAATGTGGAAACCGAGATTTCTCATGGAGAGGATGATGTAGAAAAAGCTGAAATGGATATGTTGTGGAAAGAACTTGAAGTGTCACTGGCATCGAGTTATTTTGATGACTCAGAGGTTTCAAATGCAATTGTTTTGGCCGAGCCTGAGGAAAACTTAGAAGAAGTTTGTGAACATGATAATAGATTGGATGAAGAAATTGGAATATACTGCTGTAGATGTGGCTTTGTGACCACCCATATCAGAGATGTTAATCCAATTTTTGTTGAAAATTCAGTGTGGCGCCAAGAGGAGAAGCAGTGTGATGGAGAAGATAAAGAAGAGCCCACCAAGGATGATGAAGATGATGACTTCCATTTTTTCCCGACCGACACTTCTCGTGATGAACCGATATCGGAAGAAAACCAAAGCGTTTGGTCATTAATTCCTGAACTCAGAGAAAAATTGCATGTGCACCAAAAGAAAGCTTTTGAGTTTCTTTGGCGAAACGTTGCAGGGTCTACAAATCCAGGGCTAATTGAAGCCGAATCCAAAAAAAGAGGTGGCTGTGTGATATCTCATACTCCTGGAGCCGGAAAAACTTTTCTCATCATCGCTTTTCTTGTTAGCTACCTGAAGTTATTCCCGGGGAAGAGACCTCTAGTTCTTGCTCCAAAAACTACGCTCTATACTTGGTATAAAGAATTTATCAAGTGGGAGATTCCTATACCGGTGTACTTGATTCATGGTCGTAGAACCTATAGGGTGTTCAAGCAGAATACAGTTGCTACTCTTCCTGGAGTTCCCAAGCCTACCGATGATGTCAAGCATGTTTTGGATTGCCTTGAGAAGATCCAAAAATGGCATTCACATCCGAGTGTTCTAATCATGGGCTACACTTCGTTTTTAACATTGATGCGAGAGGATTCAAAGTTTGCACACCGAAAGTTTATGGCTCAAGTGTTGAGGGAAAGTCCTGGACTCTTGGTACTTGATGAAGGTCACAACCCTAGAAGTACCAAATCAAGGTTGCGAAAATGTTTGATGAAAGTGCAAACCGAATTGAGAATATTGCTTTCCGGTACTTTGTTTCAAAATAATTTCTGCGAATACTTCAACACTCTATGCTTGGCAAGACCGAAATTCCCTCACGAAGTGTTGAAAGCATTAGACCCTAAATACAAGAGGAAAAGGAAAGGAGTAGGCCAAAAAGGAGCAGAAAAGGCGCAATATTTGATCGAGTCACGGGCAAGAAAATTCTTCTTAGATACAATCGCTAGGAAAATTGACTCTAATGTTGGAGAGGAAAGAATTCAAGGATTAAACATGTTGAGAAATGTGACAAATGGTTTTATCGATGTTTATGAAGCAGGAAGTTCTGATGGTTTACCAGGTTTACAAATTTATACACTGCTAATGAATACAACCGATATACAGCATGAGATTCTGCAGAAACTTCACAGCGACATGTTTAAGTGCAGTGGATACCCTCTCGAGTTAGAGCTTTTGATAACACTTGGATCAATACATCCTTGGCTGGTAAAAACTGCGGTGTGTTCTCAGAAATTTTTAACAAAGGAGCAATTGAGTGATCTTGATAAGTACAAGTTTGATTTGAAAATAGGTTCAAAAGTAAGATTTGTTTTGAGCCTTATCTACCGTGTTGTCAAGAACGAAAAGGTTCTTATCTTTTGCCACAACATTGCGCCTGTTCGCTTATTTCAAGAATATTTCGAGAAGTACTTTGGATGGCAAAAAGGCCGTGAAGTTTTAGTACTAACCGGGGAACTCGAGCTCTTTGAACGTGGGAAAATAATGGATAAATTTGAGGAGCCTGGTGGAGTATCAAAGATACTACTTGCTTCAATCACAGCTTGTGCCGAAGGAATTAGTTTGACAGCTGCATCTCGTGTGATCATGTTGGATTCCGAGTGGAACCCTTCGAAAACGAAGCAAGCTATTGCACGTGCGTTTCGTCCTGGTCAGCAGAAAATGGTTTATGTTTATCAGCTCTTGGTGACAGGTTCGTTGGAGGAAGATAAGTACCGAAGAACCACTTGGAAAGAGTGGGTGTCGAGCATGATTTTCAGCGAGGCGTTTGTCGAGGATCCTTCTCGTTGGCAAGCTGAGAAGATTGAAGATGATATTCTAAGGGAAATGGTTGAGGAGGATAAGTCTAAATCATTCCATATGATCATGAAGAATGAAAAGGCTTCAACAAAAACATAA |
| Protein sequence |
>MsG0580029253.01.T01 MIDKQFMCADEALTVSGNFCAKSAAEVAHIQHWQSQTMMNRKRRLNESKNSFNSDPFEAVIFGSWHPVEFIKVKSGSMSIHFTDNHHMVMDKGALSDIRIRSRKATVSDCSCFLRPGIDICVLSPPQRAGDSVGLNLEPVWADARISSIQRKPHGSECSCQFFVNFYIEQGSLGLEMRTLCKDVKVFGLNQISILQKIEHSPCENQPHRWSSSEDCSSLPNTKLLLGKFLIDLSWLVVTSVVKNVSFCARSVENKMVYQILGGDSSNSSSSNTESHIDVIGFKTDDGMLVPIVSQVAITNTKRADHARESRADEASSSYNVDGLRRSKRRHVQPERYVGCEVKELDVGTFRNMPPVRIETSKAVVDEMSLPLSFLFRLPQSSPEKGAIKCQKASKPNACRELLVYNRRAKTQKGKKTCGGVDQKEHKNPLAIIPLPDQDADPIAVEHYDPNGNVARSHEHQSRDITSQYSHLVNNPKPRKNINLLDDPAKADDAEKNDHVSSRCQFFGSTKLQRKSTGSLDDMDLGNRWEGIKRKSKTGFHEGTYRSTHLRNNGEGRSHNYKDRTLNAAAYKSLIHSYLQNINTIPVIEEPPITDQWKKCNTTNGVGQNVETEISHGEDDVEKAEMDMLWKELEVSLASSYFDDSEVSNAIVLAEPEENLEEVCEHDNRLDEEIGIYCCRCGFVTTHIRDVNPIFVENSVWRQEEKQCDGEDKEEPTKDDEDDDFHFFPTDTSRDEPISEENQSVWSLIPELREKLHVHQKKAFEFLWRNVAGSTNPGLIEAESKKRGGCVISHTPGAGKTFLIIAFLVSYLKLFPGKRPLVLAPKTTLYTWYKEFIKWEIPIPVYLIHGRRTYRVFKQNTVATLPGVPKPTDDVKHVLDCLEKIQKWHSHPSVLIMGYTSFLTLMREDSKFAHRKFMAQVLRESPGLLVLDEGHNPRSTKSRLRKCLMKVQTELRILLSGTLFQNNFCEYFNTLCLARPKFPHEVLKALDPKYKRKRKGVGQKGAEKAQYLIESRARKFFLDTIARKIDSNVGEERIQGLNMLRNVTNGFIDVYEAGSSDGLPGLQIYTLLMNTTDIQHEILQKLHSDMFKCSGYPLELELLITLGSIHPWLVKTAVCSQKFLTKEQLSDLDKYKFDLKIGSKVRFVLSLIYRVVKNEKVLIFCHNIAPVRLFQEYFEKYFGWQKGREVLVLTGELELFERGKIMDKFEEPGGVSKILLASITACAEGISLTAASRVIMLDSEWNPSKTKQAIARAFRPGQQKMVYVYQLLVTGSLEEDKYRRTTWKEWVSSMIFSEAFVEDPSRWQAEKIEDDILREMVEEDKSKSFHMIMKNEKASTKT* |
