AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029807.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029807.01.T02 MTR_5g093010 97.931 145 3 0 1 145 1 145 2.51e-106 299
MsG0580029807.01.T02 MTR_7g028905 82.906 117 20 0 1 117 1 117 1.75e-71 210
MsG0580029807.01.T02 MTR_5g083230 51.376 109 53 0 6 114 7 115 1.49e-38 129
MsG0580029807.01.T02 MTR_3g031660 45.833 120 62 1 6 125 8 124 3.72e-37 125
MsG0580029807.01.T02 MTR_2g068760 48.571 105 54 0 6 110 14 118 5.04e-37 124
MsG0580029807.01.T02 MTR_3g071590 42.143 140 73 2 6 145 9 140 7.29e-37 124
MsG0580029807.01.T02 MTR_4g083680 52.525 99 47 0 6 104 16 114 1.89e-35 121
MsG0580029807.01.T02 MTR_5g083960 45.669 127 66 1 6 129 40 166 8.51e-34 117
MsG0580029807.01.T02 MTR_3g094690 50.000 106 53 0 6 111 48 153 9.11e-33 115
MsG0580029807.01.T02 MTR_3g452660 49.074 108 55 0 6 113 8 115 1.54e-32 116
MsG0580029807.01.T02 MTR_3g073690 45.631 103 56 0 6 108 10 112 4.88e-32 113
MsG0580029807.01.T02 MTR_4g060950 42.857 112 63 1 1 111 20 131 7.95e-32 112
MsG0580029807.01.T02 MTR_7g033800 45.690 116 59 1 6 117 12 127 8.46e-32 111
MsG0580029807.01.T02 MTR_4g105170 47.664 107 56 0 6 112 12 118 8.88e-32 112
MsG0580029807.01.T02 MTR_2g093310 38.298 141 77 2 1 141 29 159 1.15e-31 112
MsG0580029807.01.T02 MTR_6g005070 48.000 100 52 0 6 105 10 109 8.31e-31 108
MsG0580029807.01.T02 MTR_3g094690 50.000 106 52 1 6 111 48 152 1.03e-30 110
MsG0580029807.01.T02 MTR_6g005080 40.876 137 74 2 6 142 11 140 1.21e-30 107
MsG0580029807.01.T02 MTR_5g017950 45.631 103 56 0 6 108 9 111 1.22e-30 112
MsG0580029807.01.T02 MTR_3g077240 49.000 100 51 0 6 105 11 110 1.73e-30 108
MsG0580029807.01.T02 MTR_5g075020 48.077 104 54 0 6 109 44 147 1.00e-28 105
MsG0580029807.01.T02 MTR_1g095850 43.564 101 57 0 6 106 73 173 1.09e-28 105
MsG0580029807.01.T02 MTR_5g075020 48.077 104 54 0 6 109 48 151 1.56e-28 105
MsG0580029807.01.T02 MTR_6g011250 37.879 132 79 2 6 134 10 141 1.98e-28 103
MsG0580029807.01.T02 MTR_8g040900 56.250 80 35 0 6 85 8 87 2.77e-28 104
MsG0580029807.01.T02 MTR_3g071420 44.681 94 52 0 6 99 15 108 3.06e-28 103
MsG0580029807.01.T02 MTR_6g027710 44.340 106 58 1 6 110 41 146 1.25e-27 102
MsG0580029807.01.T02 MTR_5g083010 41.584 101 59 0 6 106 12 112 1.32e-27 101
MsG0580029807.01.T02 MTR_7g096530 45.098 102 55 1 6 106 15 116 3.78e-27 101
MsG0580029807.01.T02 MTR_6g011230 41.237 97 57 0 6 102 12 108 8.05e-27 99.8
MsG0580029807.01.T02 MTR_1g070220 43.478 115 60 2 6 119 16 126 1.12e-26 99.8
MsG0580029807.01.T02 MTR_8g036085 41.667 108 62 1 4 110 22 129 1.49e-25 97.4
MsG0580029807.01.T02 MTR_7g074990 44.554 101 55 1 6 105 21 121 2.64e-25 96.7
MsG0580029807.01.T02 MTR_7g096610 41.732 127 67 3 6 125 9 135 9.87e-25 95.1
MsG0580029807.01.T02 MTR_8g079660 39.604 101 61 0 6 106 9 109 1.05e-24 95.9
MsG0580029807.01.T02 MTR_8g036105 43.269 104 58 1 4 106 17 120 2.02e-24 94.0
MsG0580029807.01.T02 MTR_1g070205 37.857 140 78 4 6 144 13 144 4.22e-24 93.6
MsG0580029807.01.T02 MTR_7g075230 37.063 143 84 4 4 140 11 153 1.09e-23 91.7
MsG0580029807.01.T02 MTR_8g079580 42.574 101 57 1 6 105 9 109 1.11e-23 92.4
MsG0580029807.01.T02 MTR_5g080470 41.176 102 59 1 6 106 8 109 2.20e-23 90.9
MsG0580029807.01.T02 MTR_6g027700 35.878 131 71 2 6 123 8 138 1.62e-22 89.0
MsG0580029807.01.T02 MTR_8g079620 36.634 101 64 0 6 106 9 109 2.49e-22 89.0
MsG0580029807.01.T02 MTR_6g011200 35.294 102 65 1 6 106 10 111 3.98e-22 88.6
MsG0580029807.01.T02 MTR_5g085390 33.333 144 87 4 6 144 10 149 1.21e-21 87.0
MsG0580029807.01.T02 MTR_4g107450 40.000 100 60 0 6 105 11 110 1.34e-20 85.5
MsG0580029807.01.T02 MTR_8g017090 36.538 104 66 0 6 109 13 116 3.67e-20 84.3
MsG0580029807.01.T02 MTR_4g099230 34.314 102 66 1 6 106 3 104 4.26e-20 81.3
MsG0580029807.01.T02 MTR_2g100020 33.663 101 67 0 6 106 20 120 5.88e-19 80.9
MsG0580029807.01.T02 MTR_4g088035 31.395 86 59 0 6 91 7 92 2.53e-13 65.5
MsG0580029807.01.T01 MTR_5g093010 95.205 146 6 1 1 146 1 145 1.43e-101 287
MsG0580029807.01.T01 MTR_7g028905 79.661 118 23 1 1 118 1 117 1.02e-66 198
MsG0580029807.01.T01 MTR_5g083230 49.091 110 55 1 6 115 7 115 1.09e-34 119
MsG0580029807.01.T01 MTR_3g031660 43.802 121 64 2 6 126 8 124 3.45e-33 115
MsG0580029807.01.T01 MTR_2g068760 46.226 106 56 1 6 111 14 118 3.93e-33 114
MsG0580029807.01.T01 MTR_3g071590 40.426 141 75 3 6 146 9 140 6.28e-33 114
MsG0580029807.01.T01 MTR_4g083680 50.000 100 49 1 6 105 16 114 1.43e-31 111
MsG0580029807.01.T01 MTR_4g105170 49.074 108 54 1 6 113 12 118 1.90e-31 111
MsG0580029807.01.T01 MTR_5g083960 45.312 128 66 2 6 130 40 166 2.36e-31 111
MsG0580029807.01.T01 MTR_7g033800 47.009 117 57 2 6 118 12 127 2.07e-30 108
MsG0580029807.01.T01 MTR_6g005070 49.505 101 50 1 6 106 10 109 2.90e-30 107
MsG0580029807.01.T01 MTR_6g005080 49.505 101 50 1 6 106 11 110 5.41e-30 106
MsG0580029807.01.T01 MTR_3g452660 48.624 109 55 1 6 114 8 115 5.97e-30 110
MsG0580029807.01.T01 MTR_3g077240 49.505 101 50 1 6 106 11 110 7.69e-30 107
MsG0580029807.01.T01 MTR_4g060950 41.593 113 64 2 1 112 20 131 1.02e-29 107
MsG0580029807.01.T01 MTR_2g093310 37.324 142 78 3 1 142 29 159 1.32e-29 107
MsG0580029807.01.T01 MTR_3g094690 47.664 107 55 1 6 112 48 153 2.22e-29 107
MsG0580029807.01.T01 MTR_3g073690 45.192 104 56 1 6 109 10 112 3.06e-29 106
MsG0580029807.01.T01 MTR_3g094690 47.664 107 54 1 6 112 48 152 6.82e-29 106
MsG0580029807.01.T01 MTR_6g011250 38.636 132 79 1 6 135 10 141 7.43e-29 104
MsG0580029807.01.T01 MTR_5g017950 45.192 104 56 1 6 109 9 111 6.07e-28 105
MsG0580029807.01.T01 MTR_8g040900 56.790 81 34 1 6 86 8 87 4.79e-27 101
MsG0580029807.01.T01 MTR_1g095850 42.157 102 58 1 6 107 73 173 1.27e-26 100
MsG0580029807.01.T01 MTR_5g075020 47.619 105 54 1 6 110 44 147 2.38e-26 99.4
MsG0580029807.01.T01 MTR_5g075020 47.619 105 54 1 6 110 48 151 3.69e-26 99.0
MsG0580029807.01.T01 MTR_3g071420 43.158 95 53 1 6 100 15 108 6.29e-26 97.1
MsG0580029807.01.T01 MTR_5g083010 40.196 102 60 1 6 107 12 112 5.07e-25 94.7
MsG0580029807.01.T01 MTR_7g096530 44.660 103 55 2 6 107 15 116 1.05e-24 94.7
MsG0580029807.01.T01 MTR_6g027710 42.991 107 59 2 6 111 41 146 2.75e-24 94.0
MsG0580029807.01.T01 MTR_6g011230 40.816 98 57 1 6 103 12 108 6.78e-24 92.4
MsG0580029807.01.T01 MTR_1g070220 41.379 116 62 3 6 120 16 126 2.42e-23 90.9
MsG0580029807.01.T01 MTR_7g074990 44.118 102 55 2 6 106 21 121 7.78e-23 90.5
MsG0580029807.01.T01 MTR_8g036085 41.284 109 62 2 4 111 22 129 1.80e-22 89.4
MsG0580029807.01.T01 MTR_5g085390 33.333 144 88 3 6 145 10 149 3.27e-22 88.6
MsG0580029807.01.T01 MTR_8g079660 38.235 102 62 1 6 107 9 109 6.32e-22 88.6
MsG0580029807.01.T01 MTR_7g096610 41.406 128 67 4 6 126 9 135 7.38e-22 87.4
MsG0580029807.01.T01 MTR_8g036105 42.857 105 58 2 4 107 17 120 1.27e-21 86.7
MsG0580029807.01.T01 MTR_8g079580 41.748 103 56 3 6 106 9 109 3.09e-21 85.9
MsG0580029807.01.T01 MTR_6g011200 34.314 102 67 0 6 107 10 111 3.38e-21 86.3
MsG0580029807.01.T01 MTR_1g070205 37.589 141 78 5 6 145 13 144 3.68e-21 85.9
MsG0580029807.01.T01 MTR_7g075230 36.806 144 84 5 4 141 11 153 6.02e-21 84.7
MsG0580029807.01.T01 MTR_5g080470 41.748 103 58 2 6 107 8 109 8.47e-21 84.0
MsG0580029807.01.T01 MTR_8g079620 37.255 102 63 1 6 107 9 109 8.74e-21 85.1
MsG0580029807.01.T01 MTR_4g099230 33.333 102 68 0 6 107 3 104 9.94e-20 80.5
MsG0580029807.01.T01 MTR_6g027700 34.848 132 72 3 6 124 8 138 2.75e-19 80.5
MsG0580029807.01.T01 MTR_4g107450 39.604 101 60 1 6 106 11 110 1.04e-18 80.5
MsG0580029807.01.T01 MTR_8g017090 36.275 102 64 1 6 107 13 113 3.22e-17 76.6
MsG0580029807.01.T01 MTR_2g100020 32.353 102 68 1 6 107 20 120 1.09e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029807.01.T02 AT3G26620 64.706 102 36 0 5 106 5 106 1.42e-45 145
MsG0580029807.01.T02 AT3G26660 64.706 102 36 0 5 106 5 106 1.45e-45 145
MsG0580029807.01.T02 AT2G30130 50.926 108 53 0 6 113 9 116 9.42e-38 127
MsG0580029807.01.T02 AT1G31320 60.465 86 34 0 6 91 14 99 5.42e-35 120
MsG0580029807.01.T02 AT5G63090 42.029 138 80 0 6 143 12 149 1.90e-32 114
MsG0580029807.01.T02 AT5G63090 42.029 138 80 0 6 143 12 149 1.90e-32 114
MsG0580029807.01.T02 AT5G63090 42.029 138 80 0 6 143 12 149 1.90e-32 114
MsG0580029807.01.T02 AT5G63090 42.029 138 80 0 6 143 12 149 1.90e-32 114
MsG0580029807.01.T02 AT3G27650 49.000 100 51 0 6 105 40 139 3.17e-32 112
MsG0580029807.01.T02 AT2G28500 42.342 111 64 0 1 111 51 161 1.23e-31 113
MsG0580029807.01.T02 AT1G07900 42.342 111 64 0 1 111 29 139 1.37e-31 111
MsG0580029807.01.T02 AT1G16530 49.505 101 50 1 6 105 15 115 1.63e-31 110
MsG0580029807.01.T02 AT2G30340 47.170 106 56 0 6 111 53 158 2.37e-31 113
MsG0580029807.01.T02 AT2G30340 47.170 106 56 0 6 111 54 159 2.47e-31 113
MsG0580029807.01.T02 AT2G23660 45.631 103 56 0 6 108 6 108 6.78e-31 113
MsG0580029807.01.T02 AT2G23660 45.631 103 56 0 6 108 6 108 6.78e-31 113
MsG0580029807.01.T02 AT2G23660 45.631 103 56 0 6 108 6 108 6.78e-31 113
MsG0580029807.01.T02 AT5G66870 44.660 103 57 0 6 108 8 110 1.02e-30 112
MsG0580029807.01.T02 AT2G40470 45.385 130 65 2 6 135 21 144 2.93e-30 108
MsG0580029807.01.T02 AT2G40470 45.736 129 64 2 6 134 46 168 4.58e-30 108
MsG0580029807.01.T02 AT3G11090 35.294 136 80 1 6 141 12 139 1.11e-28 103
MsG0580029807.01.T02 AT1G65620 45.545 101 55 0 6 106 10 110 5.51e-28 102
MsG0580029807.01.T02 AT1G65620 45.545 101 55 0 6 106 10 110 5.51e-28 102
MsG0580029807.01.T02 AT1G65620 45.545 101 55 0 6 106 10 110 5.51e-28 102
MsG0580029807.01.T02 AT1G65620 45.545 101 55 0 6 106 10 110 5.51e-28 102
MsG0580029807.01.T02 AT1G65620 45.545 101 55 0 6 106 10 110 5.51e-28 102
MsG0580029807.01.T02 AT4G00210 39.416 137 73 3 4 132 10 144 1.15e-27 102
MsG0580029807.01.T02 AT3G03760 45.545 101 54 1 6 105 52 152 4.63e-27 102
MsG0580029807.01.T02 AT2G42430 43.810 105 58 1 6 109 16 120 1.22e-26 100
MsG0580029807.01.T02 AT3G50510 41.905 105 61 0 2 106 9 113 3.89e-26 98.2
MsG0580029807.01.T02 AT3G50510 41.905 105 61 0 2 106 9 113 3.89e-26 98.2
MsG0580029807.01.T02 AT5G06080 41.322 121 67 2 6 125 8 125 2.06e-25 95.5
MsG0580029807.01.T02 AT2G45410 45.000 100 54 1 8 106 19 118 8.09e-25 94.4
MsG0580029807.01.T02 AT3G58190 43.137 102 57 1 6 106 12 113 5.64e-24 92.8
MsG0580029807.01.T02 AT4G00220 40.385 104 61 1 8 110 20 123 5.92e-24 93.2
MsG0580029807.01.T02 AT2G42440 43.000 100 56 1 6 104 8 107 7.47e-24 93.2
MsG0580029807.01.T02 AT3G47870 37.736 106 66 0 4 109 35 140 2.64e-23 93.2
MsG0580029807.01.T02 AT2G45420 42.308 104 59 1 8 110 40 143 3.62e-23 91.7
MsG0580029807.01.T02 AT3G13850 33.766 154 82 2 6 139 37 190 1.11e-22 90.5
MsG0580029807.01.T02 AT5G35900 40.777 103 54 1 6 101 6 108 1.29e-20 84.0
MsG0580029807.01.T02 AT2G31310 31.206 141 89 3 6 145 8 141 3.06e-20 82.4
MsG0580029807.01.T02 AT1G72980 33.077 130 83 2 6 134 14 140 8.86e-18 76.6
MsG0580029807.01.T02 AT1G06280 37.209 86 54 0 6 91 25 110 1.09e-17 76.3
MsG0580029807.01.T02 AT2G19820 34.021 97 58 2 6 101 13 104 5.86e-15 67.0
MsG0580029807.01.T02 AT4G22700 31.579 114 76 2 1 113 1 113 2.64e-13 64.3
MsG0580029807.01.T02 AT5G15060 43.243 74 38 2 6 76 18 90 8.32e-13 62.0
MsG0580029807.01.T02 AT1G36000 27.957 93 67 0 6 98 10 102 8.13e-12 58.9
MsG0580029807.01.T01 AT3G26660 62.136 103 38 1 5 107 5 106 6.65e-42 135
MsG0580029807.01.T01 AT3G26620 62.136 103 38 1 5 107 5 106 9.23e-42 135
MsG0580029807.01.T01 AT2G30130 48.624 109 55 1 6 114 9 116 9.30e-34 117
MsG0580029807.01.T01 AT5G63090 43.796 137 76 1 6 142 12 147 4.19e-32 113
MsG0580029807.01.T01 AT5G63090 43.796 137 76 1 6 142 12 147 4.19e-32 113
MsG0580029807.01.T01 AT5G63090 43.796 137 76 1 6 142 12 147 4.19e-32 113
MsG0580029807.01.T01 AT5G63090 43.796 137 76 1 6 142 12 147 4.19e-32 113
MsG0580029807.01.T01 AT1G31320 57.471 87 36 1 6 92 14 99 3.56e-31 110
MsG0580029807.01.T01 AT3G27650 49.505 101 50 1 6 106 40 139 1.70e-30 108
MsG0580029807.01.T01 AT2G28500 41.071 112 65 1 1 112 51 161 1.24e-29 107
MsG0580029807.01.T01 AT1G07900 41.071 112 65 1 1 112 29 139 1.76e-29 106
MsG0580029807.01.T01 AT2G23660 44.231 104 57 1 6 109 6 108 3.41e-28 105
MsG0580029807.01.T01 AT2G23660 44.231 104 57 1 6 109 6 108 3.41e-28 105
MsG0580029807.01.T01 AT2G23660 44.231 104 57 1 6 109 6 108 3.41e-28 105
MsG0580029807.01.T01 AT2G30340 44.860 107 58 1 6 112 54 159 3.66e-28 105
MsG0580029807.01.T01 AT2G30340 44.860 107 58 1 6 112 53 158 3.75e-28 105
MsG0580029807.01.T01 AT5G66870 44.231 104 57 1 6 109 8 110 6.71e-28 105
MsG0580029807.01.T01 AT1G16530 47.059 102 52 2 6 106 15 115 1.68e-27 100
MsG0580029807.01.T01 AT2G40470 43.511 131 67 3 6 136 21 144 1.33e-26 99.4
MsG0580029807.01.T01 AT2G40470 43.846 130 66 3 6 135 46 168 1.51e-26 99.8
MsG0580029807.01.T01 AT1G65620 45.098 102 55 1 6 107 10 110 8.73e-26 97.1
MsG0580029807.01.T01 AT1G65620 45.098 102 55 1 6 107 10 110 8.73e-26 97.1
MsG0580029807.01.T01 AT1G65620 45.098 102 55 1 6 107 10 110 8.73e-26 97.1
MsG0580029807.01.T01 AT1G65620 45.098 102 55 1 6 107 10 110 8.73e-26 97.1
MsG0580029807.01.T01 AT1G65620 45.098 102 55 1 6 107 10 110 8.73e-26 97.1
MsG0580029807.01.T01 AT3G11090 40.385 104 61 1 6 109 12 114 1.41e-25 95.9
MsG0580029807.01.T01 AT4G00210 39.130 138 73 4 4 133 10 144 9.61e-25 95.1
MsG0580029807.01.T01 AT3G50510 42.453 106 60 1 2 107 9 113 1.49e-24 94.0
MsG0580029807.01.T01 AT3G50510 42.453 106 60 1 2 107 9 113 1.49e-24 94.0
MsG0580029807.01.T01 AT2G42430 43.396 106 58 2 6 110 16 120 3.13e-24 94.4
MsG0580029807.01.T01 AT3G03760 45.098 102 54 2 6 106 52 152 4.80e-24 94.4
MsG0580029807.01.T01 AT5G06080 40.164 122 68 3 6 126 8 125 2.45e-22 87.8
MsG0580029807.01.T01 AT3G47870 37.383 107 66 1 4 110 35 140 1.69e-21 88.6
MsG0580029807.01.T01 AT2G45410 42.574 101 56 2 8 107 19 118 1.81e-21 85.9
MsG0580029807.01.T01 AT3G58190 42.718 103 57 2 6 107 12 113 5.73e-21 85.1
MsG0580029807.01.T01 AT2G42440 42.574 101 56 2 6 105 8 107 6.13e-21 85.5
MsG0580029807.01.T01 AT4G00220 40.000 105 61 2 8 111 20 123 7.24e-21 85.1
MsG0580029807.01.T01 AT2G45420 41.905 105 59 2 8 111 40 143 1.87e-20 84.7
MsG0580029807.01.T01 AT3G13850 32.903 155 83 3 6 140 37 190 2.77e-20 84.3
MsG0580029807.01.T01 AT5G35900 39.423 104 55 2 6 102 6 108 1.18e-18 79.0
MsG0580029807.01.T01 AT2G31310 30.986 142 89 4 6 146 8 141 1.28e-17 75.9
MsG0580029807.01.T01 AT2G19820 31.959 97 61 1 6 102 13 104 1.98e-15 68.6
MsG0580029807.01.T01 AT1G06280 35.632 87 55 1 6 92 25 110 5.85e-15 68.9
MsG0580029807.01.T01 AT1G72980 31.298 131 85 3 6 135 14 140 1.21e-14 68.6
MsG0580029807.01.T01 AT4G22700 31.304 115 76 3 1 114 1 113 1.94e-12 62.0

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTCATATTGTTGATTT+TGG 0.095309 5:+102191029 None:intergenic
AGGATTCAAAATCCTGTTTA+TGG 0.168254 5:-102191145 MsG0580029807.01.T02:CDS
AGCAAATCTTTGAGGATTAT+TGG 0.230583 5:+102191407 None:intergenic
AGCAATCTGAGTTTGGATTT+TGG 0.249357 5:+102191063 None:intergenic
GGATTATTGGCAGGGAAATA+TGG 0.290635 5:+102191420 None:intergenic
GGCCCAATCAAACACCTAAT+TGG 0.314557 5:-102190952 MsG0580029807.01.T02:CDS
GCTTCTGTCCACCGAATCTA+TGG 0.338967 5:-102191387 MsG0580029807.01.T02:CDS
TATGACAAGCAATCTGAGTT+TGG 0.340516 5:+102191056 None:intergenic
AAGTCTTAATTGAACCAATT+AGG 0.341976 5:+102190938 None:intergenic
GAACCAATTAGGTGTTTGAT+TGG 0.385252 5:+102190949 None:intergenic
CCAGCTCAGCTTCTGTATCA+TGG 0.391779 5:+102191086 None:intergenic
ATCCCAACACATCCATAAAC+AGG 0.441067 5:+102191133 None:intergenic
AATCTTTGAGGATTATTGGC+AGG 0.455276 5:+102191411 None:intergenic
TGCTCCATGCTGCTACTTTG+TGG 0.458851 5:+102190983 None:intergenic
AACCAATTAGGTGTTTGATT+GGG 0.459934 5:+102190950 None:intergenic
CCATGATACAGAAGCTGAGC+TGG 0.490473 5:-102191086 MsG0580029807.01.T02:CDS
TGTATTTAGAAGCACAATGT+AGG 0.494601 5:-102191165 MsG0580029807.01.T02:CDS
TGGACAGAAGCAAATCTTTG+AGG 0.503992 5:+102191399 None:intergenic
CAAAATCAACAATATGAAGC+TGG 0.506425 5:-102191028 MsG0580029807.01.T02:CDS
ATCTATGGTGGTGCCAATGT+TGG 0.519520 5:-102191372 MsG0580029807.01.T02:CDS
CATTGGCACCACCATAGATT+CGG 0.530883 5:+102191376 None:intergenic
TTGACATCATGATTTATGGT+AGG 0.535888 5:-102191497 None:intergenic
AATCCTGTTTATGGATGTGT+TGG 0.538555 5:-102191136 MsG0580029807.01.T02:CDS
GGAGAGAAAATGCAATCAGA+AGG 0.551169 5:+102191441 None:intergenic
CTTGCAAGAGTCAAAGAAGA+AGG 0.555730 5:-102191467 MsG0580029807.01.T02:CDS
ATCTTTGAGGATTATTGGCA+GGG 0.557843 5:+102191412 None:intergenic
GCCTCCACAAAGTAGCAGCA+TGG 0.580606 5:-102190987 MsG0580029807.01.T02:CDS
ATCCTGTTTATGGATGTGTT+GGG 0.594258 5:-102191135 MsG0580029807.01.T02:CDS
GCTTGTTCTCTTACATAGTG+AGG 0.601870 5:+102191199 None:intergenic
GAGAGAAAATGCAATCAGAA+GGG 0.619697 5:+102191442 None:intergenic
TGGCACCACCATAGATTCGG+TGG 0.632816 5:+102191379 None:intergenic
TCTGTCCACCGAATCTATGG+TGG 0.637607 5:-102191384 MsG0580029807.01.T02:CDS
TCCATGCTGCTACTTTGTGG+AGG 0.665689 5:+102190986 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAATATAATTATAACATA+TGG - Chr5:102191142-102191161 MsG0580029807.01.T02:CDS 10.0%
!!! TTCTTATCTAGTATTTTTAT+TGG - Chr5:102191199-102191218 MsG0580029807.01.T02:CDS 15.0%
AACCAATTAGGTGTTTGATT+GGG + Chr5:102191485-102191504 None:intergenic 30.0%
AGGATTCAAAATCCTGTTTA+TGG - Chr5:102191287-102191306 MsG0580029807.01.T02:intron 30.0%
CAAAATCAACAATATGAAGC+TGG - Chr5:102191404-102191423 MsG0580029807.01.T02:CDS 30.0%
! AGCAAATCTTTGAGGATTAT+TGG + Chr5:102191028-102191047 None:intergenic 30.0%
! TGTATTTAGAAGCACAATGT+AGG - Chr5:102191267-102191286 MsG0580029807.01.T02:intron 30.0%
!!! CAGCTTCATATTGTTGATTT+TGG + Chr5:102191406-102191425 None:intergenic 30.0%
AATCCTGTTTATGGATGTGT+TGG - Chr5:102191296-102191315 MsG0580029807.01.T02:intron 35.0%
ATCCTGTTTATGGATGTGTT+GGG - Chr5:102191297-102191316 MsG0580029807.01.T02:intron 35.0%
CAATGTTGGAAAAATGCTTC+AGG - Chr5:102191074-102191093 MsG0580029807.01.T02:CDS 35.0%
GAACCAATTAGGTGTTTGAT+TGG + Chr5:102191486-102191505 None:intergenic 35.0%
GAGAGAAAATGCAATCAGAA+GGG + Chr5:102190993-102191012 None:intergenic 35.0%
TATGACAAGCAATCTGAGTT+TGG + Chr5:102191379-102191398 None:intergenic 35.0%
! AATCTTTGAGGATTATTGGC+AGG + Chr5:102191024-102191043 None:intergenic 35.0%
! AGCAATCTGAGTTTGGATTT+TGG + Chr5:102191372-102191391 None:intergenic 35.0%
! ATCTTTGAGGATTATTGGCA+GGG + Chr5:102191023-102191042 None:intergenic 35.0%
!! CTGAAGCATTTTTCCAACAT+TGG + Chr5:102191076-102191095 None:intergenic 35.0%
ATCCCAACACATCCATAAAC+AGG + Chr5:102191302-102191321 None:intergenic 40.0%
CTTGCAAGAGTCAAAGAAGA+AGG - Chr5:102190965-102190984 MsG0580029807.01.T02:CDS 40.0%
GCTTGTTCTCTTACATAGTG+AGG + Chr5:102191236-102191255 None:intergenic 40.0%
GGAGAGAAAATGCAATCAGA+AGG + Chr5:102190994-102191013 None:intergenic 40.0%
GGATTATTGGCAGGGAAATA+TGG + Chr5:102191015-102191034 None:intergenic 40.0%
TGGACAGAAGCAAATCTTTG+AGG + Chr5:102191036-102191055 None:intergenic 40.0%
CATTGGCACCACCATAGATT+CGG + Chr5:102191059-102191078 None:intergenic 45.0%
GGCCCAATCAAACACCTAAT+TGG - Chr5:102191480-102191499 MsG0580029807.01.T02:CDS 45.0%
! ATCTATGGTGGTGCCAATGT+TGG - Chr5:102191060-102191079 MsG0580029807.01.T02:CDS 45.0%
!! GCAGCATGGAGCATTTTCAA+TGG - Chr5:102191459-102191478 MsG0580029807.01.T02:CDS 45.0%
CCAGCTCAGCTTCTGTATCA+TGG + Chr5:102191349-102191368 None:intergenic 50.0%
CCATGATACAGAAGCTGAGC+TGG - Chr5:102191346-102191365 MsG0580029807.01.T02:intron 50.0%
GCTTCTGTCCACCGAATCTA+TGG - Chr5:102191045-102191064 MsG0580029807.01.T02:CDS 50.0%
TCTGTCCACCGAATCTATGG+TGG - Chr5:102191048-102191067 MsG0580029807.01.T02:CDS 50.0%
!! TCCATGCTGCTACTTTGTGG+AGG + Chr5:102191449-102191468 None:intergenic 50.0%
!! TGCTCCATGCTGCTACTTTG+TGG + Chr5:102191452-102191471 None:intergenic 50.0%
GCCTCCACAAAGTAGCAGCA+TGG - Chr5:102191445-102191464 MsG0580029807.01.T02:CDS 55.0%
TGGCACCACCATAGATTCGG+TGG + Chr5:102191056-102191075 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 102190943 102191511 102190943 ID=MsG0580029807.01;Name=MsG0580029807.01
Chr5 mRNA 102190943 102191511 102190943 ID=MsG0580029807.01.T01;Parent=MsG0580029807.01;Name=MsG0580029807.01.T01;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|0|0|2|0|146
Chr5 exon 102190943 102191244 102190943 ID=MsG0580029807.01.T01:exon:28239;Parent=MsG0580029807.01.T01
Chr5 exon 102191373 102191511 102191373 ID=MsG0580029807.01.T01:exon:28238;Parent=MsG0580029807.01.T01
Chr5 CDS 102191373 102191511 102191373 ID=MsG0580029807.01.T01:cds;Parent=MsG0580029807.01.T01
Chr5 CDS 102190943 102191244 102190943 ID=MsG0580029807.01.T01:cds;Parent=MsG0580029807.01.T01
Chr5 mRNA 102190943 102191511 102190943 ID=MsG0580029807.01.T02;Parent=MsG0580029807.01;Name=MsG0580029807.01.T02;_AED=0.11;_eAED=0.12;_QI=0|0|0|1|0|0|2|0|145
Chr5 exon 102190943 102191227 102190943 ID=MsG0580029807.01.T02:exon:28241;Parent=MsG0580029807.01.T02
Chr5 exon 102191359 102191511 102191359 ID=MsG0580029807.01.T02:exon:28240;Parent=MsG0580029807.01.T02
Chr5 CDS 102191359 102191511 102191359 ID=MsG0580029807.01.T02:cds;Parent=MsG0580029807.01.T02
Chr5 CDS 102190943 102191227 102190943 ID=MsG0580029807.01.T02:cds;Parent=MsG0580029807.01.T02
Gene Sequence

>MsG0580029807.01.T02

ATGATTTATGGTAGGTGTGCTGCTTGCAAGAGTCAAAGAAGAAGGTGCCCTTCTGATTGCATTTTCTCTCCATATTTCCCTGCCAATAATCCTCAAAGATTTGCTTCTGTCCACCGAATCTATGGTGGTGCCAATGTTGGAAAAATGCTTCAGCAATTGCCTCACTATGTAAGAGAACAAGCTGCAAATACTTTGTATTTAGAAGCACAATGTAGGATTCAAAATCCTGTTTATGGATGTGTTGGGATCATCTCTAAATTGTATCAACAAATCCATGATACAGAAGCTGAGCTGGCCAAAATCCAAACTCAGATTGCTTGTCATAAGCTCCAAAATCAACAATATGAAGCTGGATCAAACTTCAATCTTTTGCCTCCACAAAGTAGCAGCATGGAGCATTTTCAATGGCCCAATCAAACACCTAATTGGTTCAATTAA

>MsG0580029807.01.T01

ATGATTTATGGTAGGTGTGCTGCTTGCAAGAGTCAAAGAAGAAGGTGCCCTTCTGATTGCATTTTCTCTCCATATTTCCCTGCCAATAATCCTCAAAGATTTGCTTCTGTCCACCGAATCTATGGTGGTGCCAATGTTGTATTTTTATTGGTGCAGCAATTGCCTCACTATGTAAGAGAACAAGCTGCAAATACTTTGTATTTAGAAGCACAATGTAGGATTCAAAATCCTGTTTATGGATGTGTTGGGATCATCTCTAAATTGTATCAACAAATCCATGATACAGAAGCTGAGCTGGCCAAAATCCAAACTCAGATTGCTTGTCATAAGCTCCAAAATCAACAATATGAAGCTGGATCAAACTTCAATCTTTTGCCTCCACAAAGTAGCAGCATGGAGCATTTTCAATGGCCCAATCAAACACCTAATTGGTTCAATTAA
Protein sequence

>MsG0580029807.01.T02

MIYGRCAACKSQRRRCPSDCIFSPYFPANNPQRFASVHRIYGGANVGKMLQQLPHYVREQAANTLYLEAQCRIQNPVYGCVGIISKLYQQIHDTEAELAKIQTQIACHKLQNQQYEAGSNFNLLPPQSSSMEHFQWPNQTPNWFN*

>MsG0580029807.01.T01

MIYGRCAACKSQRRRCPSDCIFSPYFPANNPQRFASVHRIYGGANVVFLLVQQLPHYVREQAANTLYLEAQCRIQNPVYGCVGIISKLYQQIHDTEAELAKIQTQIACHKLQNQQYEAGSNFNLLPPQSSSMEHFQWPNQTPNWFN*