AlfalfaGEDB | MsG0580029966.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029966.01

KEGG: K08065
GO: GO:0005634 GO:0006355 GO:0043565 GO:0046982
PFAM: PF00808

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029966.01.T01	AFK49667.1	97.857	140	0	1	1	137	1	140	7.10E-94	279

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029966.01.T01	O23310	86.777	121	14	1	1	121	1	119	3.10E-74	221

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029966.01.T01	I3TAX5	97.857	140	0	1	1	137	1	140	3.39e-94	279

TFs/TRs:

Gene ID	Type	Classification
MsG0580029966.01.T01	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029966.01.T01	MTR_5g095740	97.857	140	0	1	1	137	1	140	1.42e-97	278
MsG0580029966.01.T01	MTR_3g058980	86.861	137	14	1	1	133	1	137	1.80e-82	240
MsG0580029966.01.T01	MTR_2g026710	82.353	119	15	1	1	119	1	113	1.33e-67	202
MsG0580029966.01.T01	MTR_8g091720	80.342	117	21	1	10	124	13	129	3.43e-65	197
MsG0580029966.01.T01	MTR_1g072790	68.182	132	36	1	1	126	1	132	1.18e-62	190
MsG0580029966.01.T01	MTR_7g061270	75.207	121	24	2	9	125	10	128	3.75e-62	188
MsG0580029966.01.T01	MTR_7g100650	71.654	127	29	2	6	125	2	128	5.42e-62	188
MsG0580029966.01.T01	MTR_8g093920	80.583	103	20	0	22	124	39	141	8.91e-60	184
MsG0580029966.01.T01	MTR_4g133938	74.167	120	27	2	1	120	1	116	4.99e-59	179
MsG0580029966.01.T01	MTR_4g133952	70.103	97	29	0	22	118	57	153	7.91e-51	162
MsG0580029966.01.T01	MTR_1g088860	71.875	96	27	0	22	117	35	130	2.00e-50	159
MsG0580029966.01.T01	MTR_0392s0020	62.385	109	39	1	9	117	6	112	1.79e-48	152
MsG0580029966.01.T01	MTR_4g119500	61.207	116	42	2	5	118	3	117	1.86e-48	152
MsG0580029966.01.T01	MTR_1g039040	65.347	101	35	0	22	122	5	105	7.04e-48	153
MsG0580029966.01.T01	MTR_1g083070	61.957	92	35	0	23	114	4	95	1.91e-40	132
MsG0580029966.01.T01	MTR_1g029070	51.000	100	48	1	18	116	46	145	4.90e-30	107
MsG0580029966.01.T01	MTR_5g095900	45.545	101	52	1	15	112	5	105	3.18e-23	90.1
MsG0580029966.01.T01	MTR_1g029100	43.421	76	43	0	28	103	7	82	6.28e-20	79.0
MsG0580029966.01.T01	MTR_1g028480	46.429	84	41	1	53	132	19	102	1.43e-19	78.6
MsG0580029966.01.T01	MTR_2g056000	37.179	78	49	0	24	101	42	119	6.45e-16	70.9
MsG0580029966.01.T01	MTR_4g112380	37.179	78	49	0	24	101	12	89	9.39e-16	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029966.01.T01	AT4G14540	86.777	121	14	1	1	121	1	119	3.16e-75	221
MsG0580029966.01.T01	AT5G47640	88.889	108	12	0	19	126	23	130	2.68e-68	204
MsG0580029966.01.T01	AT2G38880	70.732	123	36	0	4	126	2	124	2.63e-61	185
MsG0580029966.01.T01	AT2G38880	70.732	123	36	0	4	126	2	124	3.34e-61	185
MsG0580029966.01.T01	AT2G38880	70.732	123	36	0	4	126	2	124	3.34e-61	185
MsG0580029966.01.T01	AT2G38880	70.732	123	36	0	4	126	2	124	3.34e-61	185
MsG0580029966.01.T01	AT2G37060	66.667	135	37	2	6	134	15	147	1.37e-60	184
MsG0580029966.01.T01	AT2G37060	66.667	135	37	2	6	134	15	147	1.37e-60	184
MsG0580029966.01.T01	AT2G37060	66.667	135	37	2	6	134	15	147	1.37e-60	184
MsG0580029966.01.T01	AT2G38880	75.455	110	27	0	4	113	2	111	2.71e-58	177
MsG0580029966.01.T01	AT2G38880	69.919	123	35	1	4	126	2	122	3.25e-58	177
MsG0580029966.01.T01	AT3G53340	68.033	122	33	1	19	134	25	146	4.15e-58	178
MsG0580029966.01.T01	AT3G53340	68.033	122	33	1	19	134	25	146	4.15e-58	178
MsG0580029966.01.T01	AT2G38880	75.455	110	27	0	4	113	2	111	6.53e-58	177
MsG0580029966.01.T01	AT2G38880	75.455	110	27	0	4	113	2	111	6.53e-58	177
MsG0580029966.01.T01	AT2G38880	75.455	110	27	0	4	113	2	111	6.53e-58	177
MsG0580029966.01.T01	AT2G38880	75.455	110	27	0	4	113	2	111	6.67e-58	177
MsG0580029966.01.T01	AT2G38880	75.455	110	27	0	4	113	2	111	6.67e-58	177
MsG0580029966.01.T01	AT2G13570	78.218	101	22	0	22	122	35	135	2.84e-57	177
MsG0580029966.01.T01	AT3G53340	80.000	95	19	0	19	113	25	119	2.05e-56	172
MsG0580029966.01.T01	AT3G53340	80.208	96	19	0	19	114	25	120	4.93e-56	172
MsG0580029966.01.T01	AT3G53340	80.208	96	19	0	19	114	25	120	4.93e-56	172
MsG0580029966.01.T01	AT5G47670	55.882	136	54	1	6	141	12	141	2.13e-53	167
MsG0580029966.01.T01	AT5G47670	55.882	136	54	1	6	141	41	170	6.77e-53	167
MsG0580029966.01.T01	AT5G47670	55.882	136	54	1	6	141	41	170	6.77e-53	167
MsG0580029966.01.T01	AT2G47810	72.917	96	26	0	22	117	50	145	1.13e-50	159
MsG0580029966.01.T01	AT1G21970	63.366	101	37	0	22	122	58	158	1.94e-47	153
MsG0580029966.01.T01	AT1G09030	60.417	96	38	0	23	118	3	98	1.14e-42	138
MsG0580029966.01.T01	AT5G08190	39.241	79	48	0	23	101	11	89	5.79e-17	73.2
MsG0580029966.01.T01	AT5G08190	39.241	79	48	0	23	101	11	89	6.24e-17	73.2
MsG0580029966.01.T01	AT5G23090	37.179	78	49	0	24	101	12	89	2.17e-16	71.6
MsG0580029966.01.T01	AT5G23090	37.179	78	49	0	24	101	12	89	2.19e-16	71.6
MsG0580029966.01.T01	AT5G23090	37.179	78	49	0	24	101	12	89	2.19e-16	71.6
MsG0580029966.01.T01	AT5G23090	37.179	78	49	0	24	101	12	89	2.19e-16	71.6
MsG0580029966.01.T01	AT5G23090	37.097	62	39	0	40	101	15	76	6.36e-12	59.7

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTCTCTCTGACACTTAT+CGG	0.341007	5:-104572274	None:intergenic
AATAATGAAGAAGGCGTTAC+CGG	0.357624	5:+104572167	MsG0580029966.01.T01:CDS
ATTCTGCTCACGTTTGCTAT+TGG	0.361435	5:-104572147	None:intergenic
CGGTGATAACTCGCTGTTCC+CGG	0.363693	5:-104572103	None:intergenic
AACTCGCTGTTCCCGGCGTT+CGG	0.373131	5:-104572096	None:intergenic
TGGTGTTGCTTCTTCGTCTT+CGG	0.380106	5:+104572437	MsG0580029966.01.T01:CDS
GGCTGATTCCGACAATGAAT+CGG	0.393443	5:+104572065	MsG0580029966.01.T01:CDS
TTCGAGATCTTCGCGTTCGC+CGG	0.412847	5:-104572186	None:intergenic
TCAACGGCGACGATCTACTT+TGG	0.416392	5:+104572307	MsG0580029966.01.T01:CDS
CGGGAGGAGCACCGAACGCC+GGG	0.420650	5:+104572085	MsG0580029966.01.T01:CDS
CGTTGAAGATAAATCTTCAA+TGG	0.432633	5:-104572366	None:intergenic
AGACGCGAAGGAAACAGTTC+AGG	0.459480	5:+104572209	MsG0580029966.01.T01:CDS
CAACGGCGACGATCTACTTT+GGG	0.462526	5:+104572308	MsG0580029966.01.T01:CDS
CTTTGGGCGATGACTACGTT+AGG	0.467469	5:+104572324	MsG0580029966.01.T01:CDS
TGCTCCTCCCGATTCATTGT+CGG	0.482065	5:-104572073	None:intergenic
CGTTTGCTATTGGTAAGAAT+CGG	0.499147	5:-104572137	None:intergenic
GCTGATTCCGACAATGAATC+GGG	0.501404	5:+104572066	MsG0580029966.01.T01:CDS
ACACTTATCGGAAGCTTCGC+CGG	0.521889	5:-104572262	None:intergenic
ATCTTTATCACGTGCAGCAA+CGG	0.524172	5:-104572415	None:intergenic
TTATGGGCTCCTCCTCAAGT+TGG	0.557492	5:+104572467	MsG0580029966.01.T01:CDS
TCGGGAGGAGCACCGAACGC+CGG	0.558380	5:+104572084	MsG0580029966.01.T01:CDS
CGTGAGCAGAATAATGAAGA+AGG	0.569587	5:+104572158	MsG0580029966.01.T01:CDS
AACAGTTCAGGAATGTGTAT+CGG	0.575632	5:+104572221	MsG0580029966.01.T01:CDS
GAGAAACGAAAGACGATCAA+CGG	0.592030	5:+104572291	MsG0580029966.01.T01:CDS
GAGTTCATCAGCTTCATCAC+CGG	0.608726	5:+104572243	MsG0580029966.01.T01:CDS
TGCATGATCATTCCAACTTG+AGG	0.615795	5:-104572479	None:intergenic
GAAGATCTCGAAAGACGCGA+AGG	0.642019	5:+104572197	MsG0580029966.01.T01:CDS
AAGAATCGGTCTTGTTCTCG+CGG	0.644860	5:-104572123	None:intergenic
CAACGATTCAGAGAGATCGA+AGG	0.645752	5:+104572384	MsG0580029966.01.T01:CDS
ATGATCATTCCAACTTGAGG+AGG	0.661433	5:-104572476	None:intergenic
GTTGCTGCACGTGATAAAGA+TGG	0.679086	5:+104572417	MsG0580029966.01.T01:CDS
GATTCCGACAATGAATCGGG+AGG	0.690680	5:+104572069	MsG0580029966.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CGTTGAAGATAAATCTTCAA+TGG	-	Chr5:104572369-104572388	None:intergenic	30.0%
	AACAGTTCAGGAATGTGTAT+CGG	+	Chr5:104572221-104572240	MsG0580029966.01.T01:CDS	35.0%
	TTTCTCTCTCTGACACTTAT+CGG	-	Chr5:104572277-104572296	None:intergenic	35.0%
!!	AATAATGAAGAAGGCGTTAC+CGG	+	Chr5:104572167-104572186	MsG0580029966.01.T01:CDS	35.0%
!!	CGTTTGCTATTGGTAAGAAT+CGG	-	Chr5:104572140-104572159	None:intergenic	35.0%
	ATCTTTATCACGTGCAGCAA+CGG	-	Chr5:104572418-104572437	None:intergenic	40.0%
	CGTGAGCAGAATAATGAAGA+AGG	+	Chr5:104572158-104572177	MsG0580029966.01.T01:CDS	40.0%
	GAGAAACGAAAGACGATCAA+CGG	+	Chr5:104572291-104572310	MsG0580029966.01.T01:CDS	40.0%
!	CGTCTTCGGTTTTCGATTAT+GGG	+	Chr5:104572451-104572470	MsG0580029966.01.T01:CDS	40.0%
!	TCGTCTTCGGTTTTCGATTA+TGG	+	Chr5:104572450-104572469	MsG0580029966.01.T01:CDS	40.0%
!!	ATTCTGCTCACGTTTGCTAT+TGG	-	Chr5:104572150-104572169	None:intergenic	40.0%
	CAACGATTCAGAGAGATCGA+AGG	+	Chr5:104572384-104572403	MsG0580029966.01.T01:CDS	45.0%
	GAGTTCATCAGCTTCATCAC+CGG	+	Chr5:104572243-104572262	MsG0580029966.01.T01:CDS	45.0%
	GTTGCTGCACGTGATAAAGA+TGG	+	Chr5:104572417-104572436	MsG0580029966.01.T01:CDS	45.0%
!	GCTGATTCCGACAATGAATC+GGG	+	Chr5:104572066-104572085	MsG0580029966.01.T01:CDS	45.0%
!	GGCTGATTCCGACAATGAAT+CGG	+	Chr5:104572065-104572084	MsG0580029966.01.T01:CDS	45.0%
!!	AAGAATCGGTCTTGTTCTCG+CGG	-	Chr5:104572126-104572145	None:intergenic	45.0%
!!	TGGTGTTGCTTCTTCGTCTT+CGG	+	Chr5:104572437-104572456	MsG0580029966.01.T01:CDS	45.0%
	ACACTTATCGGAAGCTTCGC+CGG	-	Chr5:104572265-104572284	None:intergenic	50.0%
	AGACGCGAAGGAAACAGTTC+AGG	+	Chr5:104572209-104572228	MsG0580029966.01.T01:CDS	50.0%
	CAACGGCGACGATCTACTTT+GGG	+	Chr5:104572308-104572327	MsG0580029966.01.T01:CDS	50.0%
	CTTTGGGCGATGACTACGTT+AGG	+	Chr5:104572324-104572343	MsG0580029966.01.T01:CDS	50.0%
	GAAGATCTCGAAAGACGCGA+AGG	+	Chr5:104572197-104572216	MsG0580029966.01.T01:CDS	50.0%
	GATTCCGACAATGAATCGGG+AGG	+	Chr5:104572069-104572088	MsG0580029966.01.T01:CDS	50.0%
	TCAACGGCGACGATCTACTT+TGG	+	Chr5:104572307-104572326	MsG0580029966.01.T01:CDS	50.0%
	TGCTCCTCCCGATTCATTGT+CGG	-	Chr5:104572076-104572095	None:intergenic	50.0%
	TTATGGGCTCCTCCTCAAGT+TGG	+	Chr5:104572467-104572486	MsG0580029966.01.T01:CDS	50.0%
	CGGTGATAACTCGCTGTTCC+CGG	-	Chr5:104572106-104572125	None:intergenic	55.0%
	TTCGAGATCTTCGCGTTCGC+CGG	-	Chr5:104572189-104572208	None:intergenic	55.0%
	AACTCGCTGTTCCCGGCGTT+CGG	-	Chr5:104572099-104572118	None:intergenic	60.0%
!!	TCGGGAGGAGCACCGAACGC+CGG	+	Chr5:104572084-104572103	MsG0580029966.01.T01:CDS	70.0%
!!	CGGGAGGAGCACCGAACGCC+GGG	+	Chr5:104572085-104572104	MsG0580029966.01.T01:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	104572063	104572494	104572063	ID=MsG0580029966.01;Name=MsG0580029966.01
Chr5	mRNA	104572063	104572494	104572063	ID=MsG0580029966.01.T01;Parent=MsG0580029966.01;Name=MsG0580029966.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|143
Chr5	exon	104572063	104572494	104572063	ID=MsG0580029966.01.T01:exon:547;Parent=MsG0580029966.01.T01
Chr5	CDS	104572063	104572494	104572063	ID=MsG0580029966.01.T01:cds;Parent=MsG0580029966.01.T01

Gene Sequence

>MsG0580029966.01.T01

ATGGCTGATTCCGACAATGAATCGGGAGGAGCACCGAACGCCGGGAACAGCGAGTTATCACCGCGAGAACAAGACCGATTCTTACCAATAGCAAACGTGAGCAGAATAATGAAGAAGGCGTTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAGGAAACAGTTCAGGAATGTGTATCGGAGTTCATCAGCTTCATCACCGGCGAAGCTTCCGATAAGTGTCAGAGAGAGAAACGAAAGACGATCAACGGCGACGATCTACTTTGGGCGATGACTACGTTAGGATTTGAAGAGTACGTTGAACCATTGAAGATTTATCTTCAACGATTCAGAGAGATCGAAGGTGAGAAAACCGTTGCTGCACGTGATAAAGATGGTGTTGCTTCTTCGTCTTCGGTTTTCGATTATGGGCTCCTCCTCAAGTTGGAATGA

Protein sequence

>MsG0580029966.01.T01

MADSDNESGGAPNAGNSELSPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKIYLQRFREIEGEKTVAARDKDGVASSSSVFDYGLLLKLE*