AlfalfaGEDB | MsG0680030368.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030368.01

KEGG: -
GO: -
PFAM: PF03195

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030368.01.T01	XP_003618147.1	99.31	145	1	0	1	145	1	145	1.50E-102	300

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030368.01.T01	Q8L8Q3	76.423	123	25	1	3	121	33	155	2.42E-66	201

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030368.01.T01	G7KQ00	99.310	145	1	0	1	145	1	145	7.17e-103	300

TFs/TRs:

Gene ID	Type	Classification
MsG0680030368.01.T01	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030368.01.T01	MTR_6g005070	99.310	145	1	0	1	145	1	145	1.82e-106	300
MsG0680030368.01.T01	MTR_6g005080	92.143	140	11	0	1	140	2	141	2.21e-94	269
MsG0680030368.01.T01	MTR_7g033800	68.675	166	32	4	2	149	4	167	9.81e-72	213
MsG0680030368.01.T01	MTR_3g077240	64.151	159	30	2	1	132	1	159	1.14e-65	198
MsG0680030368.01.T01	MTR_4g105170	83.810	105	17	0	7	111	9	113	4.42e-64	194
MsG0680030368.01.T01	MTR_4g083680	56.522	138	56	3	8	141	14	151	1.46e-49	157
MsG0680030368.01.T01	MTR_5g083230	57.377	122	52	0	7	128	4	125	1.18e-48	154
MsG0680030368.01.T01	MTR_5g017950	67.290	107	35	0	3	109	2	108	1.59e-48	159
MsG0680030368.01.T01	MTR_3g073690	66.972	109	36	0	1	109	1	109	4.72e-48	154
MsG0680030368.01.T01	MTR_2g068760	60.714	112	43	1	8	118	12	123	5.17e-48	152
MsG0680030368.01.T01	MTR_8g040900	69.643	112	32	1	1	112	1	110	8.07e-48	154
MsG0680030368.01.T01	MTR_3g071590	51.613	155	65	4	6	151	5	158	1.41e-46	149
MsG0680030368.01.T01	MTR_3g452660	65.455	110	38	0	4	113	2	111	1.72e-46	153
MsG0680030368.01.T01	MTR_3g031660	56.637	113	49	0	7	119	5	117	9.40e-46	147
MsG0680030368.01.T01	MTR_5g083010	64.151	106	38	0	6	111	8	113	2.37e-44	144
MsG0680030368.01.T01	MTR_3g071420	68.041	97	31	0	6	102	11	107	7.64e-44	143
MsG0680030368.01.T01	MTR_2g093310	53.279	122	57	0	1	122	25	146	8.87e-44	143
MsG0680030368.01.T01	MTR_5g083960	60.577	104	41	0	6	109	36	139	1.27e-42	140
MsG0680030368.01.T01	MTR_3g094690	59.615	104	42	0	8	111	46	149	3.67e-42	140
MsG0680030368.01.T01	MTR_4g060950	47.015	134	70	1	8	140	23	156	4.97e-40	133
MsG0680030368.01.T01	MTR_3g094690	58.654	104	42	1	8	111	46	148	1.22e-39	134
MsG0680030368.01.T01	MTR_1g095850	51.327	113	55	0	10	122	73	185	1.58e-38	130
MsG0680030368.01.T01	MTR_5g075020	56.637	113	49	0	8	120	42	154	2.95e-38	130
MsG0680030368.01.T01	MTR_5g075020	56.637	113	49	0	8	120	46	158	3.97e-38	130
MsG0680030368.01.T01	MTR_7g096530	56.881	109	46	1	2	109	7	115	1.57e-37	127
MsG0680030368.01.T01	MTR_1g070220	49.296	142	64	4	6	139	12	153	1.58e-36	125
MsG0680030368.01.T01	MTR_8g036085	55.882	102	44	1	9	109	23	124	2.46e-34	120
MsG0680030368.01.T01	MTR_7g074990	58.947	95	38	1	9	102	20	114	3.97e-34	119
MsG0680030368.01.T01	MTR_8g036105	54.717	106	47	1	9	113	18	123	5.39e-34	119
MsG0680030368.01.T01	MTR_6g027710	48.276	116	57	2	8	120	39	154	1.76e-33	117
MsG0680030368.01.T01	MTR_7g028905	48.039	102	53	0	8	109	4	105	4.94e-32	110
MsG0680030368.01.T01	MTR_5g080470	52.577	97	45	1	8	103	6	102	5.40e-32	113
MsG0680030368.01.T01	MTR_8g079660	49.074	108	54	1	9	115	8	115	1.02e-31	114
MsG0680030368.01.T01	MTR_7g075230	53.704	108	47	2	9	113	12	119	2.02e-31	111
MsG0680030368.01.T01	MTR_7g096610	54.082	98	44	1	6	102	5	102	3.02e-31	112
MsG0680030368.01.T01	MTR_5g093010	48.113	106	48	1	10	115	6	104	9.47e-31	108
MsG0680030368.01.T01	MTR_1g070205	51.579	95	45	1	9	102	12	106	2.65e-30	109
MsG0680030368.01.T01	MTR_6g011200	45.918	98	52	1	7	103	7	104	9.68e-28	103
MsG0680030368.01.T01	MTR_4g107450	47.000	100	53	0	10	109	11	110	2.69e-27	103
MsG0680030368.01.T01	MTR_8g079620	43.689	103	58	0	9	111	8	110	4.96e-27	101
MsG0680030368.01.T01	MTR_6g027700	46.535	101	53	1	9	108	7	107	1.16e-26	99.8
MsG0680030368.01.T01	MTR_8g079580	47.115	104	54	1	9	111	8	111	3.13e-26	99.4
MsG0680030368.01.T01	MTR_6g011230	41.000	100	59	0	6	105	8	107	2.23e-25	96.3
MsG0680030368.01.T01	MTR_6g011250	42.857	105	59	1	6	109	6	110	1.82e-24	93.6
MsG0680030368.01.T01	MTR_2g100020	43.750	96	54	0	10	105	20	115	8.72e-24	94.0
MsG0680030368.01.T01	MTR_8g017090	40.385	104	62	0	6	109	9	112	1.60e-23	93.6
MsG0680030368.01.T01	MTR_4g099230	40.000	95	56	1	10	103	3	97	5.75e-21	83.6
MsG0680030368.01.T01	MTR_5g085390	41.837	98	56	1	7	103	7	104	2.10e-20	84.0
MsG0680030368.01.T01	MTR_4g088035	31.111	90	62	0	8	97	5	94	3.36e-15	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030368.01.T01	AT3G27650	76.423	123	25	1	3	121	33	155	2.47e-67	201
MsG0680030368.01.T01	AT5G63090	75.591	127	29	1	1	125	1	127	9.80e-65	196
MsG0680030368.01.T01	AT5G63090	75.591	127	29	1	1	125	1	127	9.80e-65	196
MsG0680030368.01.T01	AT5G63090	75.591	127	29	1	1	125	1	127	9.80e-65	196
MsG0680030368.01.T01	AT5G63090	75.591	127	29	1	1	125	1	127	9.80e-65	196
MsG0680030368.01.T01	AT2G30130	60.169	118	47	0	7	124	6	123	1.77e-49	157
MsG0680030368.01.T01	AT1G65620	66.379	116	39	0	1	116	1	116	2.14e-48	155
MsG0680030368.01.T01	AT1G65620	66.379	116	39	0	1	116	1	116	2.14e-48	155
MsG0680030368.01.T01	AT1G65620	66.379	116	39	0	1	116	1	116	2.14e-48	155
MsG0680030368.01.T01	AT1G65620	66.379	116	39	0	1	116	1	116	2.14e-48	155
MsG0680030368.01.T01	AT1G65620	66.379	116	39	0	1	116	1	116	2.14e-48	155
MsG0680030368.01.T01	AT5G66870	66.055	109	35	1	1	109	1	107	5.19e-48	157
MsG0680030368.01.T01	AT2G23660	64.762	105	37	0	6	110	2	106	7.69e-48	157
MsG0680030368.01.T01	AT2G23660	64.762	105	37	0	6	110	2	106	7.69e-48	157
MsG0680030368.01.T01	AT2G23660	64.762	105	37	0	6	110	2	106	7.69e-48	157
MsG0680030368.01.T01	AT1G31320	69.318	88	27	0	8	95	12	99	7.73e-44	142
MsG0680030368.01.T01	AT1G07900	54.783	115	52	0	8	122	32	146	1.36e-43	142
MsG0680030368.01.T01	AT2G30340	55.752	113	50	0	8	120	51	163	1.97e-43	144
MsG0680030368.01.T01	AT2G30340	55.752	113	50	0	8	120	52	164	1.98e-43	144
MsG0680030368.01.T01	AT1G16530	62.617	107	39	1	8	113	13	119	3.01e-43	140
MsG0680030368.01.T01	AT2G28500	55.652	115	51	0	8	122	54	168	1.11e-42	141
MsG0680030368.01.T01	AT3G11090	58.559	111	44	1	6	116	8	116	1.31e-41	136
MsG0680030368.01.T01	AT2G40470	60.185	108	43	0	8	115	19	126	1.26e-40	135
MsG0680030368.01.T01	AT2G40470	60.185	108	43	0	8	115	44	151	2.54e-40	135
MsG0680030368.01.T01	AT3G26660	55.000	100	45	0	10	109	6	105	2.03e-38	127
MsG0680030368.01.T01	AT3G26620	55.000	100	45	0	10	109	6	105	2.24e-38	127
MsG0680030368.01.T01	AT2G42430	52.500	120	54	2	6	124	12	129	1.08e-35	124
MsG0680030368.01.T01	AT3G03760	54.808	104	46	1	8	110	50	153	2.04e-35	124
MsG0680030368.01.T01	AT4G00220	56.311	103	44	1	12	113	20	122	4.96e-34	119
MsG0680030368.01.T01	AT5G06080	54.639	97	43	1	8	103	6	102	1.89e-33	116
MsG0680030368.01.T01	AT4G00210	54.717	106	47	1	9	113	11	116	2.07e-33	117
MsG0680030368.01.T01	AT2G45420	55.340	103	45	1	12	113	40	142	5.51e-33	117
MsG0680030368.01.T01	AT3G58190	52.083	96	45	1	9	103	11	106	2.56e-31	112
MsG0680030368.01.T01	AT2G31310	52.083	96	45	1	8	102	6	101	2.72e-31	111
MsG0680030368.01.T01	AT2G45410	56.566	99	42	1	12	109	19	117	3.65e-31	110
MsG0680030368.01.T01	AT2G42440	49.495	99	49	1	6	103	4	102	9.32e-31	111
MsG0680030368.01.T01	AT3G50510	46.602	103	55	0	7	109	10	112	9.99e-30	107
MsG0680030368.01.T01	AT3G50510	46.602	103	55	0	7	109	10	112	9.99e-30	107
MsG0680030368.01.T01	AT3G13850	43.617	94	53	0	10	103	37	130	3.07e-25	97.4
MsG0680030368.01.T01	AT5G35900	45.000	100	55	0	10	109	6	105	3.69e-25	95.9
MsG0680030368.01.T01	AT3G47870	39.423	104	63	0	6	109	33	136	1.00e-24	97.4
MsG0680030368.01.T01	AT1G72980	41.346	104	61	0	6	109	10	113	2.10e-22	89.0
MsG0680030368.01.T01	AT1G06280	45.161	93	50	1	10	102	25	116	5.12e-21	85.1
MsG0680030368.01.T01	AT4G22700	34.921	126	71	4	10	134	6	121	6.84e-17	73.9
MsG0680030368.01.T01	AT2G19820	38.542	96	57	2	6	100	9	103	2.36e-16	70.9
MsG0680030368.01.T01	AT5G15060	36.986	73	44	1	10	80	18	90	1.22e-12	61.6

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAATGTTCACAAAATATT+TGG	0.192290	6:+1151152	MsG0680030368.01.T01:CDS
GCAAACTTATGTGGCTCTTC+TGG	0.275959	6:-1151131	None:intergenic
GGCTCTTCTGGTGGGAAATA+AGG	0.297857	6:-1151119	None:intergenic
GGTTCTTCTTCTCTTGGTCT+TGG	0.305065	6:+1151383	MsG0680030368.01.T01:CDS
CATCAAGGTTCTTCTTCTCT+TGG	0.331910	6:+1151377	MsG0680030368.01.T01:CDS
TTATATATCACCTTTGTTGT+AGG	0.350339	6:-1151471	None:intergenic
GAATCATTGCTCAAATTAGA+TGG	0.418926	6:-1151437	None:intergenic
CTTGGACATGTTCATTCTGA+TGG	0.439607	6:+1151401	MsG0680030368.01.T01:CDS
ATGATTTAAGCGAATCAAAT+CGG	0.450299	6:-1151357	None:intergenic
ATTTGAGCAATGATTCTTGT+GGG	0.463152	6:+1151444	MsG0680030368.01.T01:CDS
TTGATTCGCTTAAATCATCA+AGG	0.486236	6:+1151362	MsG0680030368.01.T01:CDS
ACAACTCATTAAGCTTCAAA+AGG	0.501141	6:+1151316	MsG0680030368.01.T01:CDS
TGTGGGGATTCCTACAACAA+AGG	0.509877	6:+1151461	MsG0680030368.01.T01:CDS
TGAACATTTGCAAACTTATG+TGG	0.513216	6:-1151140	None:intergenic
AACTTATGTGGCTCTTCTGG+TGG	0.517642	6:-1151128	None:intergenic
AGGATTAAAGATCCAGTGTA+TGG	0.525146	6:+1151263	MsG0680030368.01.T01:CDS
AATTTGAGCAATGATTCTTG+TGG	0.553273	6:+1151443	MsG0680030368.01.T01:CDS
ACTGTATCTTCTCTTTGATG+AGG	0.567592	6:-1151212	None:intergenic
GATCCAGTGTATGGTTGTGT+TGG	0.578362	6:+1151272	MsG0680030368.01.T01:CDS
AGAAACTTGCAAGCAGCACA+TGG	0.591215	6:-1151062	None:intergenic
ACTTATGTGGCTCTTCTGGT+GGG	0.607526	6:-1151127	None:intergenic
TTTGAGCAATGATTCTTGTG+GGG	0.613138	6:+1151445	MsG0680030368.01.T01:CDS
TAGCTTATGAAGCTGAAGCA+AGG	0.625096	6:+1151243	MsG0680030368.01.T01:CDS
TGGAGAATTTGAGTAGCTTG+AGG	0.626001	6:-1151042	None:intergenic
GCTCCAACACAACCATACAC+TGG	0.629286	6:-1151275	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGATTTAAGCGAATCAAAT+CGG	-	Chr6:1151360-1151379	None:intergenic	25.0%
!	GCAAATGTTCACAAAATATT+TGG	+	Chr6:1151152-1151171	MsG0680030368.01.T01:CDS	25.0%
!!	TTATATATCACCTTTGTTGT+AGG	-	Chr6:1151474-1151493	None:intergenic	25.0%
	AATTTGAGCAATGATTCTTG+TGG	+	Chr6:1151443-1151462	MsG0680030368.01.T01:CDS	30.0%
	ACAACTCATTAAGCTTCAAA+AGG	+	Chr6:1151316-1151335	MsG0680030368.01.T01:CDS	30.0%
	ATTTGAGCAATGATTCTTGT+GGG	+	Chr6:1151444-1151463	MsG0680030368.01.T01:CDS	30.0%
	GAATCATTGCTCAAATTAGA+TGG	-	Chr6:1151440-1151459	None:intergenic	30.0%
	TGAACATTTGCAAACTTATG+TGG	-	Chr6:1151143-1151162	None:intergenic	30.0%
!	TTGATTCGCTTAAATCATCA+AGG	+	Chr6:1151362-1151381	MsG0680030368.01.T01:CDS	30.0%
	AAGGTGCAAAAATGCAATCT+TGG	-	Chr6:1151103-1151122	None:intergenic	35.0%
	ACTGTATCTTCTCTTTGATG+AGG	-	Chr6:1151215-1151234	None:intergenic	35.0%
	AGGATTAAAGATCCAGTGTA+TGG	+	Chr6:1151263-1151282	MsG0680030368.01.T01:CDS	35.0%
	TTTGAGCAATGATTCTTGTG+GGG	+	Chr6:1151445-1151464	MsG0680030368.01.T01:CDS	35.0%
	CATCAAGGTTCTTCTTCTCT+TGG	+	Chr6:1151377-1151396	MsG0680030368.01.T01:CDS	40.0%
	CTTGGACATGTTCATTCTGA+TGG	+	Chr6:1151401-1151420	MsG0680030368.01.T01:CDS	40.0%
	TAGCTTATGAAGCTGAAGCA+AGG	+	Chr6:1151243-1151262	MsG0680030368.01.T01:CDS	40.0%
	TGGAGAATTTGAGTAGCTTG+AGG	-	Chr6:1151045-1151064	None:intergenic	40.0%
	AACTTATGTGGCTCTTCTGG+TGG	-	Chr6:1151131-1151150	None:intergenic	45.0%
	ACTTATGTGGCTCTTCTGGT+GGG	-	Chr6:1151130-1151149	None:intergenic	45.0%
	AGAAACTTGCAAGCAGCACA+TGG	-	Chr6:1151065-1151084	None:intergenic	45.0%
	GATCCAGTGTATGGTTGTGT+TGG	+	Chr6:1151272-1151291	MsG0680030368.01.T01:CDS	45.0%
	GCAAACTTATGTGGCTCTTC+TGG	-	Chr6:1151134-1151153	None:intergenic	45.0%
	GGTTCTTCTTCTCTTGGTCT+TGG	+	Chr6:1151383-1151402	MsG0680030368.01.T01:CDS	45.0%
	TGTGGGGATTCCTACAACAA+AGG	+	Chr6:1151461-1151480	MsG0680030368.01.T01:CDS	45.0%
	GCTCCAACACAACCATACAC+TGG	-	Chr6:1151278-1151297	None:intergenic	50.0%
	GGCTCTTCTGGTGGGAAATA+AGG	-	Chr6:1151122-1151141	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	1151038	1151493	1151038	ID=MsG0680030368.01;Name=MsG0680030368.01
Chr6	mRNA	1151038	1151493	1151038	ID=MsG0680030368.01.T01;Parent=MsG0680030368.01;Name=MsG0680030368.01.T01;_AED=0.39;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|151
Chr6	exon	1151038	1151493	1151038	ID=MsG0680030368.01.T01:exon:2447;Parent=MsG0680030368.01.T01
Chr6	CDS	1151038	1151493	1151038	ID=MsG0680030368.01.T01:cds;Parent=MsG0680030368.01.T01

Gene Sequence

>MsG0680030368.01.T01

ATGGCCTCAAGCTACTCAAATTCTCCATGTGCTGCTTGCAAGTTTCTAAGAAGAAAATGCAACCAAGATTGCATTTTTGCACCTTATTTCCCACCAGAAGAGCCACATAAGTTTGCAAATGTTCACAAAATATTTGGTGCAAGCAATGTTAGCAAAATTCTTAATGAAGTTTTACCTCATCAAAGAGAAGATACAGTGAATTCTCTAGCTTATGAAGCTGAAGCAAGGATTAAAGATCCAGTGTATGGTTGTGTTGGAGCTATTTCAGTGCTTCAAAGACAACTCATTAAGCTTCAAAAGGAACTTGATGCTACAAATGCCGATTTGATTCGCTTAAATCATCAAGGTTCTTCTTCTCTTGGTCTTGGACATGTTCATTCTGATGGTTTTTTTAATTGTCCATCTAATTTGAGCAATGATTCTTGTGGGGATTCCTACAACAAAGGTGATATATAA

Protein sequence

>MsG0680030368.01.T01

MASSYSNSPCAACKFLRRKCNQDCIFAPYFPPEEPHKFANVHKIFGASNVSKILNEVLPHQREDTVNSLAYEAEARIKDPVYGCVGAISVLQRQLIKLQKELDATNADLIRLNHQGSSSLGLGHVHSDGFFNCPSNLSNDSCGDSYNKGDI*