AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030369.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030369.01.T01 MTR_6g005080 94.366 142 8 0 1 142 1 142 1.56e-100 284
MsG0680030369.01.T01 MTR_6g005070 89.286 140 15 0 2 141 1 140 5.27e-92 263
MsG0680030369.01.T01 MTR_7g033800 86.607 112 15 0 3 114 4 115 1.39e-69 207
MsG0680030369.01.T01 MTR_3g077240 79.130 115 24 0 1 115 1 115 8.98e-65 196
MsG0680030369.01.T01 MTR_4g105170 80.374 107 21 0 8 114 9 115 7.90e-63 191
MsG0680030369.01.T01 MTR_5g083230 52.055 146 59 1 8 142 4 149 3.50e-49 155
MsG0680030369.01.T01 MTR_4g083680 63.810 105 38 0 9 113 14 118 2.51e-48 153
MsG0680030369.01.T01 MTR_3g073690 64.602 113 40 0 2 114 1 113 3.15e-48 154
MsG0680030369.01.T01 MTR_8g040900 68.750 112 33 1 2 113 1 110 1.24e-47 153
MsG0680030369.01.T01 MTR_5g017950 65.455 110 38 0 4 113 2 111 1.53e-47 156
MsG0680030369.01.T01 MTR_2g068760 56.198 121 45 1 9 129 12 124 6.87e-47 149
MsG0680030369.01.T01 MTR_3g031660 53.600 125 56 1 8 130 5 129 1.71e-45 146
MsG0680030369.01.T01 MTR_3g452660 65.138 109 38 0 5 113 2 110 2.65e-45 149
MsG0680030369.01.T01 MTR_3g071590 60.714 112 42 1 7 116 5 116 1.11e-44 144
MsG0680030369.01.T01 MTR_5g083960 58.879 107 44 0 7 113 36 142 1.55e-42 139
MsG0680030369.01.T01 MTR_3g094690 60.000 105 42 0 9 113 46 150 1.94e-42 140
MsG0680030369.01.T01 MTR_2g093310 52.459 122 58 0 2 123 25 146 3.37e-42 139
MsG0680030369.01.T01 MTR_3g071420 67.010 97 32 0 7 103 11 107 3.66e-42 138
MsG0680030369.01.T01 MTR_5g083010 63.208 106 39 0 7 112 8 113 3.85e-42 138
MsG0680030369.01.T01 MTR_3g094690 59.048 105 42 1 9 113 46 149 6.70e-40 134
MsG0680030369.01.T01 MTR_4g060950 45.070 142 65 2 9 137 23 164 9.30e-40 132
MsG0680030369.01.T01 MTR_5g075020 57.143 112 48 0 9 120 42 153 5.93e-38 129
MsG0680030369.01.T01 MTR_5g075020 57.143 112 48 0 9 120 46 157 9.60e-38 128
MsG0680030369.01.T01 MTR_7g096530 58.333 108 44 1 4 110 8 115 2.82e-37 127
MsG0680030369.01.T01 MTR_1g095850 50.442 113 56 0 11 123 73 185 6.58e-37 126
MsG0680030369.01.T01 MTR_1g070220 55.085 118 50 2 7 121 12 129 3.75e-36 124
MsG0680030369.01.T01 MTR_7g074990 60.000 95 37 1 10 103 20 114 6.96e-34 119
MsG0680030369.01.T01 MTR_8g036105 56.731 104 44 1 8 110 16 119 1.89e-33 117
MsG0680030369.01.T01 MTR_8g036085 54.286 105 47 1 10 113 23 127 1.91e-33 117
MsG0680030369.01.T01 MTR_6g027710 48.276 116 57 2 9 121 39 154 1.34e-32 115
MsG0680030369.01.T01 MTR_5g080470 53.608 97 44 1 9 104 6 102 1.23e-31 111
MsG0680030369.01.T01 MTR_5g093010 49.000 100 51 0 11 110 6 105 1.72e-31 110
MsG0680030369.01.T01 MTR_7g028905 46.078 102 55 0 9 110 4 105 2.62e-31 108
MsG0680030369.01.T01 MTR_8g079660 47.115 104 55 0 10 113 8 111 9.57e-31 111
MsG0680030369.01.T01 MTR_7g096610 55.102 98 43 1 7 103 5 102 1.56e-30 109
MsG0680030369.01.T01 MTR_7g075230 53.774 106 46 2 8 110 10 115 8.36e-30 107
MsG0680030369.01.T01 MTR_1g070205 52.632 95 44 1 10 103 12 106 1.64e-29 107
MsG0680030369.01.T01 MTR_6g011200 45.918 98 52 1 8 104 7 104 7.97e-29 106
MsG0680030369.01.T01 MTR_8g079620 44.231 104 58 0 10 113 8 111 9.22e-29 105
MsG0680030369.01.T01 MTR_4g107450 47.000 100 53 0 11 110 11 110 3.19e-28 105
MsG0680030369.01.T01 MTR_6g011230 42.000 100 58 0 7 106 8 107 1.30e-26 99.4
MsG0680030369.01.T01 MTR_6g027700 47.525 101 52 1 10 109 7 107 1.67e-26 99.0
MsG0680030369.01.T01 MTR_6g011250 43.689 103 57 1 7 108 6 108 2.08e-26 98.2
MsG0680030369.01.T01 MTR_8g079580 46.667 105 55 1 10 113 8 112 6.12e-26 98.2
MsG0680030369.01.T01 MTR_8g017090 39.252 107 65 0 7 113 9 115 1.12e-23 93.6
MsG0680030369.01.T01 MTR_2g100020 42.708 96 55 0 11 106 20 115 2.43e-23 92.4
MsG0680030369.01.T01 MTR_5g085390 42.857 98 55 1 8 104 7 104 4.87e-22 88.2
MsG0680030369.01.T01 MTR_4g099230 40.000 95 56 1 11 104 3 97 1.34e-21 85.1
MsG0680030369.01.T01 MTR_4g088035 32.222 90 61 0 9 98 5 94 3.37e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030369.01.T01 AT3G27650 80.000 110 22 0 4 113 33 142 1.46e-65 197
MsG0680030369.01.T01 AT5G63090 74.400 125 30 1 2 124 1 125 3.13e-63 192
MsG0680030369.01.T01 AT5G63090 74.400 125 30 1 2 124 1 125 3.13e-63 192
MsG0680030369.01.T01 AT5G63090 74.400 125 30 1 2 124 1 125 3.13e-63 192
MsG0680030369.01.T01 AT5G63090 74.400 125 30 1 2 124 1 125 3.13e-63 192
MsG0680030369.01.T01 AT1G65620 66.379 116 39 0 2 117 1 116 1.22e-48 155
MsG0680030369.01.T01 AT1G65620 66.379 116 39 0 2 117 1 116 1.22e-48 155
MsG0680030369.01.T01 AT1G65620 66.379 116 39 0 2 117 1 116 1.22e-48 155
MsG0680030369.01.T01 AT1G65620 66.379 116 39 0 2 117 1 116 1.22e-48 155
MsG0680030369.01.T01 AT1G65620 66.379 116 39 0 2 117 1 116 1.22e-48 155
MsG0680030369.01.T01 AT2G30130 60.526 114 45 0 8 121 6 119 2.98e-48 154
MsG0680030369.01.T01 AT5G66870 63.717 113 39 1 2 114 1 111 1.47e-46 153
MsG0680030369.01.T01 AT2G23660 62.617 107 40 0 7 113 2 108 2.11e-46 153
MsG0680030369.01.T01 AT2G23660 62.617 107 40 0 7 113 2 108 2.11e-46 153
MsG0680030369.01.T01 AT2G23660 62.617 107 40 0 7 113 2 108 2.11e-46 153
MsG0680030369.01.T01 AT1G31320 69.318 88 27 0 9 96 12 99 1.16e-43 142
MsG0680030369.01.T01 AT2G30340 55.752 113 50 0 9 121 51 163 7.29e-43 142
MsG0680030369.01.T01 AT2G30340 55.752 113 50 0 9 121 52 164 7.98e-43 142
MsG0680030369.01.T01 AT1G16530 61.538 104 39 1 9 111 13 116 1.47e-41 136
MsG0680030369.01.T01 AT1G07900 48.148 135 64 1 9 137 32 166 2.97e-41 136
MsG0680030369.01.T01 AT3G11090 57.658 111 45 1 7 117 8 116 5.61e-41 134
MsG0680030369.01.T01 AT2G28500 45.860 157 62 3 9 142 54 210 8.89e-41 136
MsG0680030369.01.T01 AT2G40470 62.857 105 39 0 9 113 19 123 1.98e-40 134
MsG0680030369.01.T01 AT2G40470 62.857 105 39 0 9 113 44 148 3.65e-40 134
MsG0680030369.01.T01 AT3G26620 46.721 122 59 1 11 132 6 121 6.74e-39 128
MsG0680030369.01.T01 AT3G26660 45.902 122 60 1 11 132 6 121 3.83e-38 126
MsG0680030369.01.T01 AT3G03760 55.769 104 45 1 9 111 50 153 3.87e-35 123
MsG0680030369.01.T01 AT2G42430 47.287 129 67 1 7 134 12 140 2.28e-34 120
MsG0680030369.01.T01 AT5G06080 50.926 108 52 1 9 115 6 113 2.94e-33 115
MsG0680030369.01.T01 AT4G00210 56.863 102 43 1 10 110 11 112 8.82e-33 115
MsG0680030369.01.T01 AT4G00220 57.576 99 41 1 13 110 20 118 1.06e-32 115
MsG0680030369.01.T01 AT2G45420 57.576 99 41 1 13 110 40 138 5.58e-32 114
MsG0680030369.01.T01 AT2G45410 46.667 135 65 3 13 140 19 153 4.07e-31 110
MsG0680030369.01.T01 AT3G58190 53.125 96 44 1 10 104 11 106 1.26e-30 110
MsG0680030369.01.T01 AT2G31310 52.083 96 45 1 9 103 6 101 1.60e-30 108
MsG0680030369.01.T01 AT3G50510 45.283 106 58 0 8 113 10 115 7.09e-30 107
MsG0680030369.01.T01 AT3G50510 45.283 106 58 0 8 113 10 115 7.09e-30 107
MsG0680030369.01.T01 AT2G42440 49.495 99 49 1 7 104 4 102 1.35e-29 107
MsG0680030369.01.T01 AT3G47870 38.182 110 68 0 1 110 27 136 1.34e-25 99.4
MsG0680030369.01.T01 AT3G13850 40.777 103 61 0 11 113 37 139 2.06e-25 97.8
MsG0680030369.01.T01 AT5G35900 43.000 100 57 0 11 110 6 105 1.42e-24 94.0
MsG0680030369.01.T01 AT1G72980 40.187 107 64 0 4 110 7 113 1.20e-22 89.4
MsG0680030369.01.T01 AT1G06280 48.052 77 40 0 11 87 25 101 3.12e-20 82.8
MsG0680030369.01.T01 AT4G22700 34.921 126 80 2 11 135 6 130 5.36e-18 76.6
MsG0680030369.01.T01 AT2G19820 36.264 91 57 1 7 96 9 99 4.67e-16 70.1
MsG0680030369.01.T01 AT5G15060 36.986 73 44 1 11 81 18 90 2.05e-12 60.8
MsG0680030369.01.T01 AT3G27940 29.245 106 75 0 10 115 5 110 9.39e-12 59.3
MsG0680030369.01.T01 AT3G27940 29.245 106 75 0 10 115 5 110 9.39e-12 59.3
MsG0680030369.01.T01 AT3G27940 29.245 106 75 0 10 115 5 110 9.39e-12 59.3

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATGTTCACAAAGTATT+TGG 0.212072 6:+1171290 MsG0680030369.01.T01:CDS
GCAAACTTATGAGGCTCTTC+TGG 0.277156 6:-1171269 None:intergenic
AATCCTTGCTTCAGCTTCAT+AGG 0.299183 6:-1171384 None:intergenic
GGCTCTTCTGGTGGGAAATA+AGG 0.304156 6:-1171257 None:intergenic
GGTTCTTCTTCTCTTGGTCT+TGG 0.305065 6:+1171521 MsG0680030369.01.T01:CDS
CATGAAGGTTCTTCTTCTCT+TGG 0.312102 6:+1171515 MsG0680030369.01.T01:CDS
TTGTAGCATCAAGTTCCTTT+TGG 0.323860 6:-1171469 None:intergenic
CGCTTCAAGGACAACTCTTT+AGG 0.341714 6:+1171444 MsG0680030369.01.T01:CDS
TGAACATTTGCAAACTTATG+AGG 0.383834 6:-1171278 None:intergenic
ATGTCATTGCTGAAATTTGA+AGG 0.464467 6:-1171575 None:intergenic
GGAGCTATTTCAGCGCTTCA+AGG 0.465943 6:+1171431 MsG0680030369.01.T01:CDS
ATGAAGTCTTACCTCATCAA+AGG 0.530030 6:+1171339 MsG0680030369.01.T01:CDS
ACAACTCTTTAGGCTCCAAA+AGG 0.536437 6:+1171454 MsG0680030369.01.T01:CDS
AACTTATGAGGCTCTTCTGG+TGG 0.540828 6:-1171266 None:intergenic
TGAAGTCTTACCTCATCAAA+GGG 0.542775 6:+1171340 MsG0680030369.01.T01:CDS
AATCCAGTGTATGGTTGTGT+TGG 0.555823 6:+1171410 MsG0680030369.01.T01:CDS
TGTCATTGCTGAAATTTGAA+GGG 0.589927 6:-1171574 None:intergenic
AGAAACTTGCAAGCAGCACA+TGG 0.591215 6:-1171200 None:intergenic
ACTTATGAGGCTCTTCTGGT+GGG 0.610191 6:-1171265 None:intergenic
ACTGTATCCTCCCTTTGATG+AGG 0.610482 6:-1171350 None:intergenic
GCTCCAACACAACCATACAC+TGG 0.615806 6:-1171413 None:intergenic
TTGCCTATGAAGCTGAAGCA+AGG 0.624248 6:+1171381 MsG0680030369.01.T01:CDS
TGGAGAATTTGAGTAGCTTG+AGG 0.626001 6:-1171180 None:intergenic
AGTCTTACCTCATCAAAGGG+AGG 0.699522 6:+1171343 MsG0680030369.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGATTAAAAATCCAGTGTA+TGG + Chr6:1171401-1171420 MsG0680030369.01.T01:CDS 30.0%
ATGTCATTGCTGAAATTTGA+AGG - Chr6:1171578-1171597 None:intergenic 30.0%
TGAACATTTGCAAACTTATG+AGG - Chr6:1171281-1171300 None:intergenic 30.0%
TGTCATTGCTGAAATTTGAA+GGG - Chr6:1171577-1171596 None:intergenic 30.0%
! GCAAATGTTCACAAAGTATT+TGG + Chr6:1171290-1171309 MsG0680030369.01.T01:CDS 30.0%
!! TTGATTCGTTTTACTCATGA+AGG + Chr6:1171500-1171519 MsG0680030369.01.T01:CDS 30.0%
AAGGTGCAAAAATGCAATCT+TGG - Chr6:1171241-1171260 None:intergenic 35.0%
ATGAAGTCTTACCTCATCAA+AGG + Chr6:1171339-1171358 MsG0680030369.01.T01:CDS 35.0%
TGAAGTCTTACCTCATCAAA+GGG + Chr6:1171340-1171359 MsG0680030369.01.T01:CDS 35.0%
! TTGTAGCATCAAGTTCCTTT+TGG - Chr6:1171472-1171491 None:intergenic 35.0%
AATCCAGTGTATGGTTGTGT+TGG + Chr6:1171410-1171429 MsG0680030369.01.T01:CDS 40.0%
AATCCTTGCTTCAGCTTCAT+AGG - Chr6:1171387-1171406 None:intergenic 40.0%
ACAACTCTTTAGGCTCCAAA+AGG + Chr6:1171454-1171473 MsG0680030369.01.T01:CDS 40.0%
CATGAAGGTTCTTCTTCTCT+TGG + Chr6:1171515-1171534 MsG0680030369.01.T01:CDS 40.0%
TGGAGAATTTGAGTAGCTTG+AGG - Chr6:1171183-1171202 None:intergenic 40.0%
AACTTATGAGGCTCTTCTGG+TGG - Chr6:1171269-1171288 None:intergenic 45.0%
ACTGTATCCTCCCTTTGATG+AGG - Chr6:1171353-1171372 None:intergenic 45.0%
ACTTATGAGGCTCTTCTGGT+GGG - Chr6:1171268-1171287 None:intergenic 45.0%
AGAAACTTGCAAGCAGCACA+TGG - Chr6:1171203-1171222 None:intergenic 45.0%
AGTCTTACCTCATCAAAGGG+AGG + Chr6:1171343-1171362 MsG0680030369.01.T01:CDS 45.0%
CGCTTCAAGGACAACTCTTT+AGG + Chr6:1171444-1171463 MsG0680030369.01.T01:CDS 45.0%
GCAAACTTATGAGGCTCTTC+TGG - Chr6:1171272-1171291 None:intergenic 45.0%
GGTTCTTCTTCTCTTGGTCT+TGG + Chr6:1171521-1171540 MsG0680030369.01.T01:CDS 45.0%
TTGCCTATGAAGCTGAAGCA+AGG + Chr6:1171381-1171400 MsG0680030369.01.T01:CDS 45.0%
GCTCCAACACAACCATACAC+TGG - Chr6:1171416-1171435 None:intergenic 50.0%
GGCTCTTCTGGTGGGAAATA+AGG - Chr6:1171260-1171279 None:intergenic 50.0%
! GGAGCTATTTCAGCGCTTCA+AGG + Chr6:1171431-1171450 MsG0680030369.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 1171173 1171601 1171173 ID=MsG0680030369.01;Name=MsG0680030369.01
Chr6 mRNA 1171173 1171601 1171173 ID=MsG0680030369.01.T01;Parent=MsG0680030369.01;Name=MsG0680030369.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|142
Chr6 exon 1171173 1171601 1171173 ID=MsG0680030369.01.T01:exon:2448;Parent=MsG0680030369.01.T01
Chr6 CDS 1171173 1171601 1171173 ID=MsG0680030369.01.T01:cds;Parent=MsG0680030369.01.T01
Gene Sequence

>MsG0680030369.01.T01

ATGATGGCCTCAAGCTACTCAAATTCTCCATGTGCTGCTTGCAAGTTTCTAAGAAGAAAATGCAACCAAGATTGCATTTTTGCACCTTATTTCCCACCAGAAGAGCCTCATAAGTTTGCAAATGTTCACAAAGTATTTGGTGCAAGCAATGTTAGCAAAATTCTTAATGAAGTCTTACCTCATCAAAGGGAGGATACAGTGAATTCTCTTGCCTATGAAGCTGAAGCAAGGATTAAAAATCCAGTGTATGGTTGTGTTGGAGCTATTTCAGCGCTTCAAGGACAACTCTTTAGGCTCCAAAAGGAACTTGATGCTACAAATGCTGATTTGATTCGTTTTACTCATGAAGGTTCTTCTTCTCTTGGTCTTGGACAATTTCACTCTGATGACTTTTTAAATTACCCTTCAAATTTCAGCAATGACATGTGA
Protein sequence

>MsG0680030369.01.T01

MMASSYSNSPCAACKFLRRKCNQDCIFAPYFPPEEPHKFANVHKVFGASNVSKILNEVLPHQREDTVNSLAYEAEARIKNPVYGCVGAISALQGQLFRLQKELDATNADLIRFTHEGSSSLGLGQFHSDDFLNYPSNFSNDM*