Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030507.01.T01 | XP_024636868.1 | 85.87 | 92 | 13 | 0 | 3 | 94 | 233 | 324 | 2.59E-50 | 172 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030507.01.T01 | A0A396IHE6 | 85.870 | 92 | 13 | 0 | 3 | 94 | 233 | 324 | 1.24e-50 | 172 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0680030507.01.T01 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030507.01.T01 | MTR_4g119990 | 85.714 | 91 | 13 | 0 | 3 | 93 | 233 | 323 | 1.13e-52 | 169 |
| MsG0680030507.01.T01 | MTR_6g043890 | 64.286 | 70 | 25 | 0 | 1 | 70 | 225 | 294 | 2.80e-27 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCACAATAATTTAAAATT+TGG | 0.142889 | 6:-4290742 | MsG0680030507.01.T01:CDS |
| CATCCTGTGCAGATGTTTCT+TGG | 0.182263 | 6:+4290876 | None:intergenic |
| AAATAAATTACCTTCAAAAT+AGG | 0.207151 | 6:+4290630 | None:intergenic |
| AATAAATTACCTTCAAAATA+GGG | 0.393629 | 6:+4290631 | None:intergenic |
| TGTTTCTTGGCAAATTCAAT+TGG | 0.419212 | 6:+4290889 | None:intergenic |
| AATAATCAATTTACACTAAG+TGG | 0.456060 | 6:-4290784 | MsG0680030507.01.T01:CDS |
| CGAGAGAGAAAATAGCAACT+TGG | 0.460403 | 6:+4290671 | None:intergenic |
| AATTTACACTAAGTGGTGGT+TGG | 0.508389 | 6:-4290777 | MsG0680030507.01.T01:CDS |
| AATTTATCTAAAGTAAAAGA+TGG | 0.511672 | 6:+4290585 | None:intergenic |
| GCACAGGATGGAAGGAAGAA+AGG | 0.547170 | 6:-4290863 | MsG0680030507.01.T01:CDS |
| TTTATATTAAACAAATGCAA+TGG | 0.550607 | 6:-4290706 | MsG0680030507.01.T01:CDS |
| AAACATCTGCACAGGATGGA+AGG | 0.555297 | 6:-4290871 | MsG0680030507.01.T01:CDS |
| TTGCCAAGAAACATCTGCAC+AGG | 0.566543 | 6:-4290879 | MsG0680030507.01.T01:CDS |
| TTGTTGATAAAGACATAACA+TGG | 0.582622 | 6:-4290831 | MsG0680030507.01.T01:CDS |
| AATCAATTTACACTAAGTGG+TGG | 0.617140 | 6:-4290781 | MsG0680030507.01.T01:CDS |
| CAAGAAACATCTGCACAGGA+TGG | 0.624347 | 6:-4290875 | MsG0680030507.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATTACCTTCAAAAT+AGG | + | Chr6:4291118-4291137 | None:intergenic | 15.0% |
| !! | AATAAATTACCTTCAAAATA+GGG | + | Chr6:4291117-4291136 | None:intergenic | 15.0% |
| !! | AATATATTAAAATGGAGTTA+TGG | + | Chr6:4290631-4290650 | None:intergenic | 15.0% |
| !! | TTTATATTAAACAAATGCAA+TGG | - | Chr6:4291039-4291058 | MsG0680030507.01.T01:intron | 15.0% |
| !! | AACTCAGAATAAATACAAAA+AGG | + | Chr6:4290821-4290840 | None:intergenic | 20.0% |
| !! | AATAATCAATTTACACTAAG+TGG | - | Chr6:4290961-4290980 | MsG0680030507.01.T01:intron | 20.0% |
| !!! | ATTTTAAATTATTGTGAGCT+CGG | + | Chr6:4291002-4291021 | None:intergenic | 20.0% |
| !!! | GCTCACAATAATTTAAAATT+TGG | - | Chr6:4291003-4291022 | MsG0680030507.01.T01:intron | 20.0% |
| ! | ATAAATACAAAAAGGCCAAA+TGG | + | Chr6:4290813-4290832 | None:intergenic | 25.0% |
| ! | TTTATTCTGAGTTGTATTAC+AGG | - | Chr6:4290828-4290847 | MsG0680030507.01.T01:CDS | 25.0% |
| !! | TTGTTGATAAAGACATAACA+TGG | - | Chr6:4290914-4290933 | MsG0680030507.01.T01:intron | 25.0% |
| AATCAATTTACACTAAGTGG+TGG | - | Chr6:4290964-4290983 | MsG0680030507.01.T01:intron | 30.0% | |
| AGATGGCAATTGAAAAATAG+CGG | + | Chr6:4291146-4291165 | None:intergenic | 30.0% | |
| GTTAGAAAATTGCATCCATT+TGG | - | Chr6:4290795-4290814 | MsG0680030507.01.T01:CDS | 30.0% | |
| TCTACATGATGTATTGTGAT+TGG | - | Chr6:4290676-4290695 | MsG0680030507.01.T01:CDS | 30.0% | |
| TGTTTCTTGGCAAATTCAAT+TGG | + | Chr6:4290859-4290878 | None:intergenic | 30.0% | |
| TTCACACACACATAAACATA+TGG | + | Chr6:4290769-4290788 | None:intergenic | 30.0% | |
| AATTTACACTAAGTGGTGGT+TGG | - | Chr6:4290968-4290987 | MsG0680030507.01.T01:intron | 35.0% | |
| !!! | TGCTATTTTCTCTCTCGAAA+AGG | - | Chr6:4291080-4291099 | MsG0680030507.01.T01:intron | 35.0% |
| CGAGAGAGAAAATAGCAACT+TGG | + | Chr6:4291077-4291096 | None:intergenic | 40.0% | |
| !! | ATGAGCACTCCCTATTTTGA+AGG | - | Chr6:4291105-4291124 | MsG0680030507.01.T01:intron | 40.0% |
| AAACATCTGCACAGGATGGA+AGG | - | Chr6:4290874-4290893 | MsG0680030507.01.T01:CDS | 45.0% | |
| CAAGAAACATCTGCACAGGA+TGG | - | Chr6:4290870-4290889 | MsG0680030507.01.T01:CDS | 45.0% | |
| CATCCTGTGCAGATGTTTCT+TGG | + | Chr6:4290872-4290891 | None:intergenic | 45.0% | |
| TTGCCAAGAAACATCTGCAC+AGG | - | Chr6:4290866-4290885 | MsG0680030507.01.T01:CDS | 45.0% | |
| !! | TAAGAAATAATATATTAAAA+TGG | + | Chr6:4290639-4290658 | None:intergenic | 5.0% |
| GCACAGGATGGAAGGAAGAA+AGG | - | Chr6:4290882-4290901 | MsG0680030507.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 4290588 | 4291179 | 4290588 | ID=MsG0680030507.01;Name=MsG0680030507.01 |
| Chr6 | mRNA | 4290588 | 4291179 | 4290588 | ID=MsG0680030507.01.T01;Parent=MsG0680030507.01;Name=MsG0680030507.01.T01;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.5|0|0.5|2|0|110 |
| Chr6 | exon | 4291177 | 4291179 | 4291177 | ID=MsG0680030507.01.T01:exon:8319;Parent=MsG0680030507.01.T01 |
| Chr6 | exon | 4290588 | 4290917 | 4290588 | ID=MsG0680030507.01.T01:exon:8318;Parent=MsG0680030507.01.T01 |
| Chr6 | CDS | 4291177 | 4291179 | 4291177 | ID=MsG0680030507.01.T01:cds;Parent=MsG0680030507.01.T01 |
| Chr6 | CDS | 4290588 | 4290917 | 4290588 | ID=MsG0680030507.01.T01:cds;Parent=MsG0680030507.01.T01 |
| Gene Sequence |
| Protein sequence |
