AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030765.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030765.01.T01 MTR_6g012220 92.941 85 2 1 1 85 1 81 4.17e-52 157
MsG0680030765.01.T01 MTR_3g039810 69.512 82 22 2 1 80 1 81 1.18e-35 116
MsG0680030765.01.T01 MTR_1g077020 59.091 66 25 1 15 80 3 66 2.03e-22 85.9
MsG0680030765.01.T01 MTR_3g100650 60.000 55 22 0 18 72 77 131 3.98e-20 81.6
MsG0680030765.01.T01 MTR_2g076780 60.714 56 22 0 21 76 37 92 5.70e-20 80.5
MsG0680030765.01.T01 MTR_8g069080 56.061 66 27 1 20 83 68 133 8.92e-20 80.5
MsG0680030765.01.T01 MTR_4g134580 63.158 57 21 0 20 76 112 168 4.51e-19 79.7
MsG0680030765.01.T01 MTR_8g467340 65.455 55 17 1 18 70 61 115 1.18e-18 78.6
MsG0680030765.01.T01 MTR_7g010300 57.627 59 23 1 16 72 83 141 1.73e-18 77.8
MsG0680030765.01.T01 MTR_5g065440 56.667 60 26 0 19 78 56 115 3.31e-18 76.3
MsG0680030765.01.T01 MTR_5g020510 59.615 52 21 0 21 72 99 150 5.17e-18 76.3
MsG0680030765.01.T01 MTR_5g032600 52.459 61 29 0 20 80 77 137 5.22e-18 75.9
MsG0680030765.01.T01 MTR_6g012280 59.259 54 20 1 21 72 91 144 4.12e-16 71.2
MsG0680030765.01.T01 MTR_6g073013 50.000 60 29 1 21 79 19 78 1.14e-14 65.5
MsG0680030765.01.T01 MTR_8g442330 50.000 38 19 0 30 67 14 51 1.89e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030765.01.T01 AT3G28917 59.596 99 25 3 1 84 1 99 6.72e-31 104
MsG0680030765.01.T01 AT1G18835 60.000 90 26 3 1 83 1 87 2.72e-29 100
MsG0680030765.01.T01 AT1G74660 50.495 101 31 2 1 82 2 102 4.19e-27 95.1
MsG0680030765.01.T01 AT1G74660 50.562 89 27 1 1 72 2 90 1.79e-25 90.9
MsG0680030765.01.T01 AT4G24660 66.071 56 19 0 19 74 47 102 9.45e-23 87.4
MsG0680030765.01.T01 AT4G24660 66.071 56 19 0 19 74 47 102 1.07e-22 87.4
MsG0680030765.01.T01 AT2G02540 55.224 67 30 0 6 72 72 138 4.97e-22 87.0
MsG0680030765.01.T01 AT2G02540 55.224 67 30 0 6 72 72 138 4.97e-22 87.0
MsG0680030765.01.T01 AT1G14440 62.069 58 22 0 15 72 84 141 2.65e-21 85.1
MsG0680030765.01.T01 AT1G14440 62.069 58 22 0 15 72 84 141 2.65e-21 85.1
MsG0680030765.01.T01 AT5G15210 59.375 64 24 1 17 78 52 115 4.93e-20 81.3
MsG0680030765.01.T01 AT3G50890 54.412 68 28 1 7 71 43 110 6.65e-20 80.5
MsG0680030765.01.T01 AT1G75240 59.677 62 25 0 16 77 71 132 9.59e-20 80.9
MsG0680030765.01.T01 AT5G65410 60.000 55 22 0 17 71 71 125 4.80e-19 78.6
MsG0680030765.01.T01 AT2G18350 61.111 54 21 0 19 72 80 133 1.06e-18 77.4
MsG0680030765.01.T01 AT1G69600 63.462 52 17 1 21 70 31 82 1.32e-17 74.3
MsG0680030765.01.T01 AT3G28920 57.812 64 25 1 19 80 50 113 6.42e-16 70.5
MsG0680030765.01.T01 AT5G60480 57.407 54 21 1 18 69 34 87 2.64e-15 67.8
MsG0680030765.01.T01 AT5G39760 48.000 75 37 1 6 78 41 115 7.14e-15 67.8
MsG0680030765.01.T01 AT5G42780 58.824 51 19 1 21 71 64 112 1.27e-14 66.2
MsG0680030765.01.T01 AT1G14687 50.769 65 23 4 21 85 7 62 2.34e-14 64.3

Find 11 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGAAGTGTTGTGAAATA+TGG 0.276912 6:+8778242 MsG0680030765.01.T01:CDS
GTTGGAGGTTATGCTGTTGA+TGG 0.403038 6:+8778293 MsG0680030765.01.T01:CDS
TTTGTACCTTGTTCACTACT+TGG 0.415225 6:-8778332 None:intergenic
TATTTCACAACACTTCTTTG+TGG 0.421230 6:-8778239 None:intergenic
AGTTCTTCACCTCGCTCAAA+TGG 0.468472 6:+8778428 MsG0680030765.01.T01:CDS
AAGAATCATGCAGCTAATGT+TGG 0.502606 6:+8778275 MsG0680030765.01.T01:CDS
AGTAGTGAACAAGGTACAAA+TGG 0.504202 6:+8778335 MsG0680030765.01.T01:CDS
TGTCTCACTTGTGCTGCTTG+TGG 0.516521 6:+8778359 MsG0680030765.01.T01:CDS
AATCATGCAGCTAATGTTGG+AGG 0.567933 6:+8778278 MsG0680030765.01.T01:CDS
TTCATGCCAAGTAGTGAACA+AGG 0.658987 6:+8778326 MsG0680030765.01.T01:CDS
TTTCAAGTACCATTTGAGCG+AGG 0.671813 6:-8778437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TATTTCACAACACTTCTTTG+TGG - Chr6:8778242-8778261 None:intergenic 30.0%
!! CAAAGAAGTGTTGTGAAATA+TGG + Chr6:8778242-8778261 MsG0680030765.01.T01:CDS 30.0%
AAGAATCATGCAGCTAATGT+TGG + Chr6:8778275-8778294 MsG0680030765.01.T01:CDS 35.0%
AGTAGTGAACAAGGTACAAA+TGG + Chr6:8778335-8778354 MsG0680030765.01.T01:CDS 35.0%
TTTGTACCTTGTTCACTACT+TGG - Chr6:8778335-8778354 None:intergenic 35.0%
AATCATGCAGCTAATGTTGG+AGG + Chr6:8778278-8778297 MsG0680030765.01.T01:CDS 40.0%
TTCATGCCAAGTAGTGAACA+AGG + Chr6:8778326-8778345 MsG0680030765.01.T01:CDS 40.0%
AGTTCTTCACCTCGCTCAAA+TGG + Chr6:8778428-8778447 MsG0680030765.01.T01:CDS 45.0%
GTTGGAGGTTATGCTGTTGA+TGG + Chr6:8778293-8778312 MsG0680030765.01.T01:CDS 45.0%
!! TGTCTCACTTGTGCTGCTTG+TGG + Chr6:8778359-8778378 MsG0680030765.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 8778200 8778457 8778200 ID=MsG0680030765.01;Name=MsG0680030765.01
Chr6 mRNA 8778200 8778457 8778200 ID=MsG0680030765.01.T01;Parent=MsG0680030765.01;Name=MsG0680030765.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|85
Chr6 exon 8778200 8778457 8778200 ID=MsG0680030765.01.T01:exon:7256;Parent=MsG0680030765.01.T01
Chr6 CDS 8778200 8778457 8778200 ID=MsG0680030765.01.T01:cds;Parent=MsG0680030765.01.T01
Gene Sequence

>MsG0680030765.01.T01

ATGAGGAAGAGACAAGTAGTTGTGAGAAGATCAGAAGATCCACAAAGAAGTGTTGTGAAATATGGAGAGTGTCAAAAGAATCATGCAGCTAATGTTGGAGGTTATGCTGTTGATGGTTGTAGAGAGTTCATGCCAAGTAGTGAACAAGGTACAAATGGTTGTCTCACTTGTGCTGCTTGTGGTTGTCACAGAAACTTTCACAAAAGAGAAGTTGAAGTTGTTTCTGAGAGTTCTTCACCTCGCTCAAATGGTACTTGA
Protein sequence

>MsG0680030765.01.T01

MRKRQVVVRRSEDPQRSVVKYGECQKNHAANVGGYAVDGCREFMPSSEQGTNGCLTCAACGCHRNFHKREVEVVSESSSPRSNGT*