AlfalfaGEDB | MsG0680031534.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031534.01

KEGG: -
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031534.01.T01	ABR16522.1	70.213	94	27	1	94	187	63	155	1.13E-29	122

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031534.01.T01	Q9LVS0	74.648	71	18	0	116	186	95	165	1.79E-31	120

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031534.01.T01	A0A444XY94	80.769	78	13	1	116	193	77	152	8.38e-35	133

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TFs/TRs:

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Gene ID	Type	Classification
MsG0680031534.01.T01	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031534.01.T01	MTR_0036s0260	79.167	72	15	0	113	184	24	95	1.08e-33	119
MsG0680031534.01.T01	MTR_2g090305	76.389	72	17	0	113	184	24	95	2.67e-31	112
MsG0680031534.01.T01	MTR_2g100930	69.014	71	22	0	116	186	108	178	7.34e-31	115
MsG0680031534.01.T01	MTR_4g100630	65.823	79	27	0	106	184	84	162	9.24e-31	114
MsG0680031534.01.T01	MTR_5g037080	78.667	75	16	0	116	190	101	175	2.04e-30	113
MsG0680031534.01.T01	MTR_6g092540	71.212	66	19	0	116	181	96	161	1.03e-28	108
MsG0680031534.01.T01	MTR_4g107230	90.141	71	7	0	116	186	83	153	1.36e-28	108
MsG0680031534.01.T01	MTR_4g111975	67.647	68	22	0	116	183	137	204	7.04e-27	103
MsG0680031534.01.T01	MTR_8g101650	68.182	66	20	1	116	181	104	168	1.84e-26	102
MsG0680031534.01.T01	MTR_1g111830	66.176	68	23	0	116	183	123	190	7.48e-26	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031534.01.T01	AT5G47390	74.648	71	18	0	116	186	95	165	1.82e-32	120
MsG0680031534.01.T01	AT1G49010	63.636	77	28	0	116	192	133	209	9.68e-28	106
MsG0680031534.01.T01	AT1G70000	69.048	84	25	1	104	187	86	168	3.85e-27	103
MsG0680031534.01.T01	AT1G70000	69.048	84	25	1	104	187	86	168	3.85e-27	103
MsG0680031534.01.T01	AT5G08520	56.818	88	37	1	96	183	98	184	1.45e-26	103
MsG0680031534.01.T01	AT5G04760	59.494	79	32	0	105	183	87	165	2.84e-26	100
MsG0680031534.01.T01	AT2G38090	60.494	81	25	1	103	183	134	207	4.17e-26	102
MsG0680031534.01.T01	AT5G58900	52.273	88	42	0	96	183	120	207	7.94e-26	100
MsG0680031534.01.T01	AT1G74840	68.539	89	28	0	99	187	78	166	8.63e-26	99.8
MsG0680031534.01.T01	AT1G74840	68.539	89	28	0	99	187	78	166	8.96e-26	100

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Find 39 sgRNAs with CRISPR-Local

Find 344 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCATGGGAAAACATCAT+TGG	0.478648	6:+21816368	None:intergenic
AATTCTATGGCGGCCGAAAA+TGG	0.315265	6:-21813365	MsG0680031534.01.T01:intron
ACCCCATGCAGCAAGATTAC+TGG	0.347137	6:-21815937	MsG0680031534.01.T01:CDS
AGAAAGTTGGAAAAGGTGAT+AGG	0.453364	6:-21810758	MsG0680031534.01.T01:CDS
AGAGACTGGATCTACGGCGA+CGG	0.700084	6:+21810640	None:intergenic
AGTGGTGATATCATAGAGAC+TGG	0.518743	6:+21810626	None:intergenic
ATCAGGGATTCTGTGGACGG+AGG	0.677291	6:-21810811	MsG0680031534.01.T01:intron
CAATACAATGGTTAACCTTA+CGG	0.454620	6:-21816013	MsG0680031534.01.T01:CDS
CATGGTCCAAAGCATCCGTA+AGG	0.626535	6:+21815998	None:intergenic
CCCAAAACAAAATCGATTTG+AGG	0.503070	6:-21816309	MsG0680031534.01.T01:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGGAAATTTAATCCCT+AGG	+	Chr6:21812692-21812711	None:intergenic	30.0%
ACCATTAATTGTCTAGAGAA+AGG	+	Chr6:21815381-21815400	None:intergenic	30.0%
AGGAATCTTTCACAAAATTG+AGG	+	Chr6:21814202-21814221	None:intergenic	30.0%
ATAATTGAAGTTCGACCAAA+AGG	+	Chr6:21815352-21815371	None:intergenic	30.0%
ATGACTCATATTGTAACAGA+TGG	-	Chr6:21815769-21815788	MsG0680031534.01.T01:intron	30.0%
CAACAAAATTCAAAACAGAC+AGG	+	Chr6:21811188-21811207	None:intergenic	30.0%
CAAGAATTATTACTCGAAAG+GGG	+	Chr6:21814228-21814247	None:intergenic	30.0%
CAATACAATGGTTAACCTTA+CGG	-	Chr6:21810945-21810964	MsG0680031534.01.T01:intron	30.0%
CAGAAAAGGAAAAATACCTT+TGG	+	Chr6:21814057-21814076	None:intergenic	30.0%
CAGGGAATTTACTTGATAAA+GGG	-	Chr6:21810692-21810711	MsG0680031534.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	21810593	21816387	21810593	ID=MsG0680031534.01;Name=MsG0680031534.01
Chr6	mRNA	21810593	21816387	21810593	ID=MsG0680031534.01.T01;Parent=MsG0680031534.01;Name=MsG0680031534.01.T01;_AED=0.68;_eAED=0.68;_QI=0\|0\|0\|0.2\|0.75\|0.8\|5\|0\|193
Chr6	exon	21816310	21816387	21816310	ID=MsG0680031534.01.T01:exon:13764;Parent=MsG0680031534.01.T01
Chr6	exon	21815918	21816064	21815918	ID=MsG0680031534.01.T01:exon:13763;Parent=MsG0680031534.01.T01
Chr6	exon	21813744	21813803	21813744	ID=MsG0680031534.01.T01:exon:13762;Parent=MsG0680031534.01.T01
Chr6	exon	21813366	21813426	21813366	ID=MsG0680031534.01.T01:exon:13761;Parent=MsG0680031534.01.T01
Chr6	exon	21810593	21810828	21810593	ID=MsG0680031534.01.T01:exon:13760;Parent=MsG0680031534.01.T01
Chr6	CDS	21816310	21816387	21816310	ID=MsG0680031534.01.T01:cds;Parent=MsG0680031534.01.T01
Chr6	CDS	21815918	21816064	21815918	ID=MsG0680031534.01.T01:cds;Parent=MsG0680031534.01.T01
Chr6	CDS	21813744	21813803	21813744	ID=MsG0680031534.01.T01:cds;Parent=MsG0680031534.01.T01
Chr6	CDS	21813366	21813426	21813366	ID=MsG0680031534.01.T01:cds;Parent=MsG0680031534.01.T01
Chr6	CDS	21810593	21810828	21810593	ID=MsG0680031534.01.T01:cds;Parent=MsG0680031534.01.T01

Gene Sequence

>MsG0680031534.01.T01

ATGATGTTTTCCCATGATTTTGGAGACCAGATCAATCAATATGCAATTCTGGTAGACCCAAAACAAAATCGATTTGAGACCCAAGAAAACAATTTTGCTGGTTCATCCAATACAATGGTTAACCTTACGGATGCTTTGGACCATGTGACTAACAACGATGACAAGGATAAAGAAATGGATACTACCCCATGCAGCAAGATTACTGGGAAAAGGTCTGCTGAAAAAGTTGTGAAATGTGAAATTATCCATATGGAAGTCAAGTTTGAAGCTTCTATACTTTTTCAGCACATCATGGAAGAAGAACAAGAGCACAGTAGTAAAAAGAATTCTATGGCGGCCGAAAATGGGATTCTGTGGACGGAGGAAGAGCACAAGATGTTTTTGGTTGGATTGCAGAAAGTTGGAAAAGGTGATAGGAGAGGAATCTCAAGGAACTATGTGAAAACAAGAACGCCTACGCAGGTTGCAAGTCATGCTCAGAAGTACTCTCTGAACCAAAGTAATCTCAATCGCCGTCGCCGTAGATCCAGTCTCTATGATATCACCACTGACACGGTAATTTTTCCGGCAATCTCTTTGTAG

Protein sequence

>MsG0680031534.01.T01

MMFSHDFGDQINQYAILVDPKQNRFETQENNFAGSSNTMVNLTDALDHVTNNDDKDKEMDTTPCSKITGKRSAEKVVKCEIIHMEVKFEASILFQHIMEEEQEHSSKKNSMAAENGILWTEEEHKMFLVGLQKVGKGDRRGISRNYVKTRTPTQVASHAQKYSLNQSNLNRRRRRSSLYDITTDTVIFPAISL*