AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031601.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031601.01.T01 MTR_6g027370 97.059 170 5 0 1 170 1 170 1.14e-117 340
MsG0680031601.01.T01 MTR_6g027370 94.186 86 5 0 171 256 262 347 5.46e-48 162
MsG0680031601.01.T01 MTR_6g027360 57.647 170 51 3 1 170 1 149 7.45e-52 171
MsG0680031601.01.T01 MTR_6g027340 43.421 228 71 6 64 256 1 205 9.91e-45 149
MsG0680031601.01.T01 MTR_5g041570 56.364 110 45 1 61 170 99 205 3.50e-35 130
MsG0680031601.01.T01 MTR_4g082040 69.620 79 24 0 92 170 98 176 1.07e-34 129
MsG0680031601.01.T01 MTR_7g086960 56.667 120 46 1 57 170 41 160 1.93e-34 127
MsG0680031601.01.T01 MTR_6g074860 72.222 72 20 0 99 170 4 75 2.21e-33 121
MsG0680031601.01.T01 MTR_3g089045 67.949 78 25 0 93 170 10 87 3.69e-30 114
MsG0680031601.01.T01 MTR_4g019370 56.164 73 32 0 98 170 37 109 1.46e-24 100
MsG0680031601.01.T01 MTR_4g094982 56.164 73 32 0 98 170 4 76 7.67e-24 97.4
MsG0680031601.01.T01 MTR_5g016510 53.425 73 34 0 98 170 4 76 2.03e-23 97.1
MsG0680031601.01.T01 MTR_6g015455 52.055 73 35 0 98 170 4 76 1.45e-20 91.3
MsG0680031601.01.T01 MTR_5g082910 49.315 73 37 0 98 170 18 90 1.02e-19 86.3
MsG0680031601.01.T01 MTR_3g101290 49.315 73 37 0 98 170 36 108 7.62e-19 82.8
MsG0680031601.01.T01 MTR_3g110028 45.122 82 44 1 89 170 76 156 5.76e-18 83.6
MsG0680031601.01.T01 MTR_3g463400 41.053 95 53 1 98 192 132 223 4.03e-17 80.5
MsG0680031601.01.T01 MTR_1g026870 42.683 82 46 1 89 170 76 156 1.91e-16 79.0
MsG0680031601.01.T01 MTR_5g070020 41.053 95 50 1 72 166 101 189 8.96e-16 76.3
MsG0680031601.01.T01 MTR_7g061330 43.902 82 45 1 89 170 104 184 1.51e-15 76.3
MsG0680031601.01.T01 MTR_7g461410 43.902 82 45 1 89 170 104 184 1.51e-15 76.3
MsG0680031601.01.T01 MTR_7g061330 43.902 82 45 1 89 170 68 148 2.16e-15 75.9
MsG0680031601.01.T01 MTR_1g057980 33.333 129 82 2 42 170 27 151 2.17e-15 74.7
MsG0680031601.01.T01 MTR_7g451170 46.575 73 39 0 98 170 190 262 2.78e-15 75.1
MsG0680031601.01.T01 MTR_5g049190 44.928 69 38 0 98 166 18 86 6.02e-15 72.4
MsG0680031601.01.T01 MTR_7g037260 45.205 73 40 0 98 170 144 216 6.15e-15 73.9
MsG0680031601.01.T01 MTR_7g037130 46.575 73 39 0 98 170 144 216 1.14e-14 73.2
MsG0680031601.01.T01 MTR_4g088015 43.836 73 41 0 98 170 220 292 2.89e-14 72.4
MsG0680031601.01.T01 MTR_7g035350 44.595 74 41 0 97 170 223 296 6.23e-14 71.2
MsG0680031601.01.T01 MTR_2g067420 46.575 73 38 1 99 170 16 88 1.28e-13 69.7
MsG0680031601.01.T01 MTR_5g010650 41.463 82 47 1 89 170 101 181 3.80e-13 68.9
MsG0680031601.01.T01 MTR_3g077650 47.826 69 36 0 98 166 180 248 3.93e-13 68.9
MsG0680031601.01.T01 MTR_4g065017 45.205 73 39 1 99 170 19 91 4.39e-13 68.2
MsG0680031601.01.T01 MTR_7g010210 35.577 104 63 2 99 201 14 114 5.20e-13 68.2
MsG0680031601.01.T01 MTR_2g097910 45.205 73 39 1 99 170 22 94 5.74e-13 67.8
MsG0680031601.01.T01 MTR_4g105660 40.000 70 42 0 98 167 179 248 5.87e-13 68.2
MsG0680031601.01.T01 MTR_5g007300 42.466 73 41 1 99 170 66 138 7.06e-13 64.7
MsG0680031601.01.T01 MTR_3g074520 44.595 74 38 2 99 170 14 86 8.67e-13 67.4
MsG0680031601.01.T01 MTR_2g089450 47.143 70 34 2 99 166 14 82 8.81e-13 66.6
MsG0680031601.01.T01 MTR_7g010210 35.577 104 63 2 99 201 14 114 9.95e-13 66.6
MsG0680031601.01.T01 MTR_3g039990 34.821 112 68 3 99 208 15 123 1.27e-12 66.6
MsG0680031601.01.T01 MTR_3g028740 41.860 86 48 2 86 170 12 96 1.39e-12 67.0
MsG0680031601.01.T01 MTR_6g012690 36.538 104 62 2 99 201 14 114 1.56e-12 67.0
MsG0680031601.01.T01 MTR_0140s0030 34.483 116 71 3 95 208 10 122 1.84e-12 66.6
MsG0680031601.01.T01 MTR_0489s0020 34.483 116 71 3 95 208 10 122 1.84e-12 66.6
MsG0680031601.01.T01 MTR_7g450950 39.241 79 48 0 88 166 146 224 2.06e-12 66.2
MsG0680031601.01.T01 MTR_5g042030 42.466 73 41 1 99 170 9 81 2.25e-12 65.9
MsG0680031601.01.T01 MTR_2g089620 43.836 73 40 1 99 170 14 86 2.58e-12 65.9
MsG0680031601.01.T01 MTR_4g082230 43.836 73 40 1 99 170 14 86 3.52e-12 65.9
MsG0680031601.01.T01 MTR_6g012180 38.824 85 48 2 99 182 14 95 6.33e-12 64.7
MsG0680031601.01.T01 MTR_4g105130 29.861 144 87 5 99 232 14 153 6.58e-12 65.1
MsG0680031601.01.T01 MTR_7g111290 43.243 74 39 2 99 170 14 86 8.59e-12 64.7
MsG0680031601.01.T01 MTR_7g110830 41.096 73 42 1 99 170 14 86 9.58e-12 64.7
MsG0680031601.01.T01 MTR_1g085640 41.096 73 42 1 99 170 14 86 9.60e-12 64.7
MsG0680031601.01.T01 MTR_5g088080 41.096 73 42 1 99 170 14 86 9.76e-12 63.9
MsG0680031601.01.T01 MTR_5g088150 41.096 73 42 1 99 170 14 86 9.76e-12 63.9
MsG0680031601.01.T01 MTR_5g488210 41.096 73 42 1 99 170 14 86 9.76e-12 63.9
MsG0680031601.01.T01 MTR_5g088640 41.096 73 42 1 99 170 14 86 1.01e-11 63.9
MsG0680031601.01.T01 MTR_4g082290 43.836 73 40 1 99 170 14 86 1.10e-11 64.7
MsG0680031601.01.T01 MTR_5g488170 41.096 73 42 1 99 170 14 86 1.17e-11 63.2
MsG0680031601.01.T01 MTR_5g062790 42.466 73 41 1 99 170 11 83 1.24e-11 63.9
MsG0680031601.01.T01 MTR_8g060940 43.836 73 40 1 99 170 9 81 1.63e-11 62.8
MsG0680031601.01.T01 MTR_5g010020 38.824 85 48 2 99 182 14 95 2.20e-11 63.5
MsG0680031601.01.T01 MTR_5g029840 39.726 73 43 1 99 170 14 86 3.30e-11 62.8
MsG0680031601.01.T01 MTR_0008s0390 43.478 69 38 1 99 166 15 83 3.50e-11 62.4
MsG0680031601.01.T01 MTR_8g017440 41.892 74 40 2 99 170 14 86 3.83e-11 62.8
MsG0680031601.01.T01 MTR_4g100720 42.169 83 46 2 90 170 5 87 4.24e-11 60.5
MsG0680031601.01.T01 MTR_0251s0050 43.478 69 38 1 99 166 15 83 4.32e-11 62.0
MsG0680031601.01.T01 MTR_2g011660 42.466 73 41 1 99 170 15 87 4.35e-11 62.4
MsG0680031601.01.T01 MTR_3g461490 41.096 73 42 1 99 170 14 86 4.80e-11 62.4
MsG0680031601.01.T01 MTR_4g091490 43.836 73 40 1 99 170 15 87 4.90e-11 62.4
MsG0680031601.01.T01 MTR_3g052450 42.466 73 41 1 99 170 42 114 5.08e-11 62.8
MsG0680031601.01.T01 MTR_7g087130 33.654 104 65 2 99 201 14 114 5.26e-11 62.4
MsG0680031601.01.T01 MTR_1g083630 40.580 69 40 1 99 166 14 82 5.54e-11 62.8
MsG0680031601.01.T01 MTR_7g017260 41.333 75 41 1 99 170 27 101 5.67e-11 61.6
MsG0680031601.01.T01 MTR_5g079220 41.096 73 42 1 99 170 23 95 6.86e-11 61.6
MsG0680031601.01.T01 MTR_5g014990 43.243 74 40 2 99 170 14 87 7.47e-11 61.6
MsG0680031601.01.T01 MTR_1g085040 40.541 74 41 2 99 170 14 86 7.93e-11 62.0
MsG0680031601.01.T01 MTR_5g078930 39.241 79 47 1 93 170 5 83 8.41e-11 60.5
MsG0680031601.01.T01 MTR_7g115650 42.466 73 41 1 99 170 14 86 8.43e-11 60.5
MsG0680031601.01.T01 MTR_1g100667 31.304 115 73 3 99 210 15 126 9.75e-11 60.8
MsG0680031601.01.T01 MTR_3g077110 40.541 74 41 2 99 170 14 86 9.98e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031601.01.T01 AT1G17950 72.222 72 20 0 99 170 5 76 5.14e-34 123
MsG0680031601.01.T01 AT1G69560 73.611 72 19 0 99 170 107 178 2.22e-33 124
MsG0680031601.01.T01 AT1G69560 73.611 72 19 0 99 170 123 194 2.54e-33 124
MsG0680031601.01.T01 AT1G26780 73.611 72 19 0 99 170 98 169 7.02e-33 121
MsG0680031601.01.T01 AT5G17800 69.231 78 24 0 93 170 87 164 2.07e-32 121
MsG0680031601.01.T01 AT1G26780 73.611 72 19 0 99 170 98 169 2.20e-32 122
MsG0680031601.01.T01 AT3G29020 63.636 77 28 0 94 170 60 136 8.19e-30 112
MsG0680031601.01.T01 AT1G73410 73.611 72 19 0 99 170 6 77 8.46e-30 112
MsG0680031601.01.T01 AT1G73410 73.611 72 19 0 99 170 6 77 9.77e-30 111
MsG0680031601.01.T01 AT4G33450 61.728 81 30 1 91 170 10 90 1.56e-29 112
MsG0680031601.01.T01 AT3G29020 63.636 77 28 0 94 170 62 138 4.02e-29 112
MsG0680031601.01.T01 AT5G39700 60.563 71 28 0 100 170 57 127 1.25e-23 94.7
MsG0680031601.01.T01 AT3G55730 57.534 73 31 0 98 170 55 127 1.59e-23 98.6
MsG0680031601.01.T01 AT5G67300 53.425 73 34 0 98 170 5 77 1.08e-22 94.7
MsG0680031601.01.T01 AT4G37260 54.795 73 33 0 98 170 12 84 1.50e-22 94.7
MsG0680031601.01.T01 AT2G39880 51.899 79 38 0 92 170 43 121 3.03e-21 91.7
MsG0680031601.01.T01 AT3G50060 47.945 73 38 0 98 170 5 77 5.39e-21 90.1
MsG0680031601.01.T01 AT2G23290 50.685 73 36 0 98 170 12 84 5.12e-20 87.8
MsG0680031601.01.T01 AT3G09230 50.685 73 36 0 98 170 54 126 1.01e-19 87.8
MsG0680031601.01.T01 AT3G09370 47.945 73 38 0 98 170 129 201 1.00e-16 79.7
MsG0680031601.01.T01 AT3G09370 47.945 73 38 0 98 170 134 206 1.02e-16 79.7
MsG0680031601.01.T01 AT3G09370 47.945 73 38 0 98 170 142 214 1.03e-16 79.7
MsG0680031601.01.T01 AT5G11510 41.463 82 47 1 89 170 72 152 8.32e-16 77.4
MsG0680031601.01.T01 AT5G11510 41.463 82 47 1 89 170 72 152 8.32e-16 77.4
MsG0680031601.01.T01 AT5G11510 41.463 82 47 1 89 170 72 152 8.32e-16 77.4
MsG0680031601.01.T01 AT5G11510 41.463 82 47 1 89 170 72 152 1.08e-15 77.0
MsG0680031601.01.T01 AT5G11510 42.466 73 42 0 98 170 80 152 1.21e-15 76.6
MsG0680031601.01.T01 AT5G11050 49.315 73 37 0 98 170 104 176 1.34e-15 76.3
MsG0680031601.01.T01 AT4G32730 41.463 82 47 1 89 170 78 158 1.71e-15 76.3
MsG0680031601.01.T01 AT4G32730 41.463 82 47 1 89 170 78 158 1.71e-15 76.3
MsG0680031601.01.T01 AT4G32730 41.463 82 47 1 89 170 78 158 1.71e-15 76.3
MsG0680031601.01.T01 AT4G32730 41.463 82 47 1 89 170 78 158 1.71e-15 76.3
MsG0680031601.01.T01 AT4G32730 41.463 82 47 1 89 170 78 158 1.80e-15 76.3
MsG0680031601.01.T01 AT5G02320 42.466 73 42 0 98 170 126 198 1.26e-14 73.6
MsG0680031601.01.T01 AT5G02320 42.466 73 42 0 98 170 126 198 1.26e-14 73.6
MsG0680031601.01.T01 AT5G02320 42.466 73 42 0 98 170 66 138 1.48e-14 73.2
MsG0680031601.01.T01 AT5G62470 36.036 111 67 2 99 208 14 121 1.50e-14 72.8
MsG0680031601.01.T01 AT3G18100 44.444 72 40 0 99 170 546 617 2.64e-14 72.8
MsG0680031601.01.T01 AT3G18100 44.444 72 40 0 99 170 498 569 2.77e-14 72.8
MsG0680031601.01.T01 AT5G58850 41.379 87 51 0 80 166 86 172 2.93e-14 72.4
MsG0680031601.01.T01 AT3G18100 44.444 72 40 0 99 170 385 456 4.11e-14 72.0
MsG0680031601.01.T01 AT3G18100 44.444 72 40 0 99 170 333 404 4.43e-14 72.0
MsG0680031601.01.T01 AT3G27785 28.415 183 104 6 50 220 136 303 4.54e-14 71.6
MsG0680031601.01.T01 AT3G01140 30.822 146 94 3 99 240 14 156 4.68e-14 71.2
MsG0680031601.01.T01 AT5G40360 43.056 72 41 0 99 170 158 229 6.21e-14 70.9
MsG0680031601.01.T01 AT3G01140 30.822 146 94 3 99 240 57 199 6.40e-14 71.2
MsG0680031601.01.T01 AT3G47600 34.074 135 84 3 99 231 14 145 9.69e-14 70.1
MsG0680031601.01.T01 AT2G25230 45.070 71 37 2 101 170 28 97 9.86e-14 68.9
MsG0680031601.01.T01 AT1G18570 43.836 73 40 1 99 170 15 87 1.52e-13 69.7
MsG0680031601.01.T01 AT1G63910 33.333 135 83 4 99 232 14 142 3.06e-13 68.9
MsG0680031601.01.T01 AT5G62470 35.714 112 67 3 99 208 14 122 4.80e-13 68.6
MsG0680031601.01.T01 AT1G79180 45.205 73 39 1 99 170 16 88 4.93e-13 67.8
MsG0680031601.01.T01 AT3G28910 35.965 114 68 3 99 210 14 124 1.18e-12 67.0
MsG0680031601.01.T01 AT5G54230 43.836 73 40 1 99 170 14 86 1.28e-12 67.0
MsG0680031601.01.T01 AT4G17785 32.857 140 81 5 99 229 15 150 1.29e-12 67.0
MsG0680031601.01.T01 AT4G17785 32.857 140 81 5 99 229 15 150 1.37e-12 67.0
MsG0680031601.01.T01 AT3G12720 38.144 97 56 3 76 170 2 96 1.54e-12 66.6
MsG0680031601.01.T01 AT4G01680 41.096 73 42 1 99 170 14 86 2.22e-12 65.5
MsG0680031601.01.T01 AT4G05100 43.836 73 40 1 99 170 15 87 2.57e-12 66.2
MsG0680031601.01.T01 AT4G01680 41.096 73 42 1 99 170 14 86 2.61e-12 66.2
MsG0680031601.01.T01 AT4G28110 44.595 74 38 2 99 170 14 86 2.78e-12 65.5
MsG0680031601.01.T01 AT1G34670 43.836 73 40 1 99 170 14 86 3.29e-12 65.9
MsG0680031601.01.T01 AT4G18770 42.029 69 40 0 98 166 216 284 3.83e-12 65.9
MsG0680031601.01.T01 AT5G15310 43.243 74 39 2 99 170 14 86 4.35e-12 65.5
MsG0680031601.01.T01 AT4G26930 39.726 73 43 1 99 170 21 93 4.38e-12 65.9
MsG0680031601.01.T01 AT5G60890 42.466 73 41 1 99 170 14 86 4.52e-12 65.1
MsG0680031601.01.T01 AT5G15310 43.243 74 39 2 99 170 14 86 4.59e-12 65.1
MsG0680031601.01.T01 AT5G52600 38.462 91 50 2 81 170 1 86 5.97e-12 63.5
MsG0680031601.01.T01 AT1G74080 41.096 73 42 1 99 170 14 86 7.37e-12 64.7
MsG0680031601.01.T01 AT1G74650 34.615 104 64 2 99 201 14 114 7.47e-12 64.7
MsG0680031601.01.T01 AT3G48920 39.000 100 50 3 72 170 3 92 9.48e-12 63.9
MsG0680031601.01.T01 AT5G40330 42.466 73 41 1 99 170 14 86 1.07e-11 63.2
MsG0680031601.01.T01 AT3G08500 42.466 73 41 1 99 170 32 104 1.19e-11 64.3
MsG0680031601.01.T01 AT3G23250 33.784 148 87 5 99 238 14 158 1.22e-11 63.9
MsG0680031601.01.T01 AT5G15310 43.243 74 39 2 99 170 14 86 1.31e-11 63.2
MsG0680031601.01.T01 AT5G10280 43.243 74 39 2 99 170 14 86 1.33e-11 63.9
MsG0680031601.01.T01 AT3G53200 43.243 74 39 2 99 170 11 83 1.38e-11 63.2
MsG0680031601.01.T01 AT5G57620 45.714 70 36 2 99 166 14 83 1.42e-11 63.9
MsG0680031601.01.T01 AT5G14750 42.466 73 41 1 99 170 18 90 1.46e-11 62.4
MsG0680031601.01.T01 AT1G08810 31.250 144 93 4 99 239 14 154 1.46e-11 63.5
MsG0680031601.01.T01 AT2G26960 26.115 157 109 3 98 250 21 174 1.55e-11 64.3
MsG0680031601.01.T01 AT5G12870 41.096 73 42 1 99 170 20 92 1.69e-11 63.2
MsG0680031601.01.T01 AT1G56160 41.892 74 40 2 99 170 16 88 2.03e-11 63.2
MsG0680031601.01.T01 AT4G01680 37.647 85 40 2 99 170 14 98 2.11e-11 63.5
MsG0680031601.01.T01 AT3G11440 41.096 73 42 1 99 170 43 115 2.18e-11 63.9
MsG0680031601.01.T01 AT3G11440 41.096 73 42 1 99 170 43 115 2.18e-11 63.9
MsG0680031601.01.T01 AT3G11440 41.096 73 42 1 99 170 43 115 2.18e-11 63.9
MsG0680031601.01.T01 AT5G65230 44.595 74 38 2 99 170 14 86 2.57e-11 63.2
MsG0680031601.01.T01 AT3G11440 41.096 73 42 1 99 170 43 115 2.71e-11 63.5
MsG0680031601.01.T01 AT1G18710 39.726 73 43 1 99 170 14 86 3.13e-11 62.4
MsG0680031601.01.T01 AT3G02940 42.466 73 41 1 99 170 14 86 3.32e-11 62.8
MsG0680031601.01.T01 AT5G07690 42.466 73 41 1 99 170 14 86 3.35e-11 62.8
MsG0680031601.01.T01 AT3G02940 42.466 73 41 1 99 170 14 86 4.49e-11 62.4
MsG0680031601.01.T01 AT3G02940 42.466 73 41 1 99 170 14 86 4.49e-11 62.4
MsG0680031601.01.T01 AT4G21440 42.466 73 41 1 99 170 14 86 4.55e-11 62.4
MsG0680031601.01.T01 AT5G07700 42.466 73 41 1 99 170 14 86 4.70e-11 62.4
MsG0680031601.01.T01 AT5G07700 42.466 73 41 1 99 170 14 86 4.70e-11 62.4
MsG0680031601.01.T01 AT3G13890 37.805 82 41 2 99 170 14 95 4.80e-11 62.4
MsG0680031601.01.T01 AT5G40430 37.500 72 44 1 99 170 54 124 5.01e-11 61.6
MsG0680031601.01.T01 AT1G74430 41.096 73 42 1 99 170 14 86 5.18e-11 62.0
MsG0680031601.01.T01 AT5G61420 41.096 73 42 1 99 170 14 86 5.56e-11 62.4
MsG0680031601.01.T01 AT3G27920 36.735 98 52 3 84 177 7 98 5.68e-11 61.2
MsG0680031601.01.T01 AT5G16770 42.466 73 41 1 99 170 14 86 6.93e-11 62.0
MsG0680031601.01.T01 AT5G16770 42.466 73 41 1 99 170 14 86 6.93e-11 62.0

Find 56 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAACAATGAATCAATTT+TGG 0.098317 6:+23003122 MsG0680031601.01.T01:CDS
TTCATCTTCTACTCCTCTTT+TGG 0.139727 6:+23001243 MsG0680031601.01.T01:CDS
AAGGAAGAAAATGAGAAATT+TGG 0.160898 6:+23001412 MsG0680031601.01.T01:CDS
AAGGGAAAAGTTGCAGATTA+AGG 0.276686 6:+23002026 MsG0680031601.01.T01:intron
GTGTCATTTATTGACTTTCT+TGG 0.290615 6:+23003177 MsG0680031601.01.T01:CDS
TCTCATTGCACTGCAAATTC+TGG 0.302223 6:-23002998 None:intergenic
TTGCATGCTCATACACTTCA+TGG 0.308993 6:+23002115 MsG0680031601.01.T01:CDS
TGATGGTTATGGTTTCTCTT+TGG 0.310144 6:+23001183 MsG0680031601.01.T01:CDS
AAAGCTGCTCTATTGATTCT+AGG 0.313747 6:-23002067 None:intergenic
AACACCAAGCTTTCCATTAG+AGG 0.318928 6:+23001436 MsG0680031601.01.T01:CDS
TGGTCTCCAATGACCTCTAA+TGG 0.346646 6:-23001449 None:intergenic
ATGAATTTGGTCCTCACAAT+TGG 0.357978 6:+23001503 MsG0680031601.01.T01:CDS
AGGAAGAAAATGAGAAATTT+GGG 0.359459 6:+23001413 MsG0680031601.01.T01:CDS
GGAAAGTTCAAAATACCCTT+TGG 0.375377 6:+23001289 MsG0680031601.01.T01:CDS
GAAAGTTCAAAATACCCTTT+GGG 0.383315 6:+23001290 MsG0680031601.01.T01:CDS
AGCTTTCCATTAGAGGTCAT+TGG 0.384561 6:+23001443 MsG0680031601.01.T01:CDS
TGAGTTTGAATATGAATGAT+TGG 0.387864 6:-23003067 None:intergenic
ATAGAAAGAAAGGTAGAAGT+AGG 0.391467 6:+23002961 MsG0680031601.01.T01:CDS
CATATTGGTAACAATGAGTC+TGG 0.398380 6:+23001268 MsG0680031601.01.T01:CDS
AAATGTGAGAGATAAGATTA+AGG 0.412885 6:+23003155 MsG0680031601.01.T01:CDS
AACTTGAACAATGATGGTTA+TGG 0.426752 6:+23001172 MsG0680031601.01.T01:CDS
AGGCTTACTTATAGAAAGAA+AGG 0.428586 6:+23002951 MsG0680031601.01.T01:intron
AAGGTAGAAGTAGGGGACTA+TGG 0.428911 6:+23002970 MsG0680031601.01.T01:CDS
GATGAAGAAGGAAGTGGAGA+TGG 0.450833 6:-23001226 None:intergenic
TCACTAAACTTGAACAATGA+TGG 0.458857 6:+23001166 MsG0680031601.01.T01:CDS
GTAGAAGATGAAGAAGGAAG+TGG 0.470505 6:-23001232 None:intergenic
AGAAGGAAGTGGAGATGGAA+GGG 0.471954 6:-23001221 None:intergenic
TAGAAAGAAAGGTAGAAGTA+GGG 0.480588 6:+23002962 MsG0680031601.01.T01:CDS
TGACCATGCTCTCATTAGGA+TGG 0.490702 6:-23001333 None:intergenic
GTTCAAGTTTAGTGAACTCA+TGG 0.494602 6:-23001158 None:intergenic
TCTTGGAGTAGGTGATACAT+AGG 0.495278 6:+23003194 MsG0680031601.01.T01:CDS
GAACAAATACATGGAAGATC+GGG 0.507355 6:+23001538 MsG0680031601.01.T01:CDS
TTTATTGACTTTCTTGGAGT+AGG 0.507502 6:+23003183 MsG0680031601.01.T01:CDS
AAGAAGGAAGTGGAGATGGA+AGG 0.512851 6:-23001222 None:intergenic
TGAACAAATACATGGAAGAT+CGG 0.519885 6:+23001537 MsG0680031601.01.T01:CDS
TTGAATATGAATGATTGGAT+TGG 0.524412 6:-23003062 None:intergenic
ATGACCTCTAATGGAAAGCT+TGG 0.534815 6:-23001440 None:intergenic
AGAAAGAAAGGTAGAAGTAG+GGG 0.536632 6:+23002963 MsG0680031601.01.T01:CDS
CTTGGAGTAGGTGATACATA+GGG 0.552083 6:+23003195 MsG0680031601.01.T01:CDS
TTAAGCTTAGCATCTTCAGA+TGG 0.553691 6:-23001469 None:intergenic
AGAGGAGTAGAAGATGAAGA+AGG 0.558498 6:-23001238 None:intergenic
AGGTTGTTCTCAAGGTCACA+TGG 0.563554 6:+23003020 MsG0680031601.01.T01:CDS
TCATTAGGATGGATCACTGA+AGG 0.567180 6:-23001322 None:intergenic
AACATTGCTGAACAAATACA+TGG 0.572771 6:+23001529 MsG0680031601.01.T01:CDS
GCAATGAGAGGTTGTTCTCA+AGG 0.574721 6:+23003012 MsG0680031601.01.T01:CDS
CTTGTCAATGAAAGTTGACA+AGG 0.581911 6:+23001393 MsG0680031601.01.T01:CDS
TTACCAATATGACCCAAAAG+AGG 0.582661 6:-23001256 None:intergenic
GCAATGTTGTTCCAATTGTG+AGG 0.589775 6:-23001514 None:intergenic
CATTAGGATGGATCACTGAA+GGG 0.599580 6:-23001321 None:intergenic
GAAGGGTTAGAAATCCCAAA+GGG 0.605125 6:-23001304 None:intergenic
GATCCATCCTAATGAGAGCA+TGG 0.615775 6:+23001330 MsG0680031601.01.T01:CDS
CATGGGAAAGCTTAAGAGTG+TGG 0.638298 6:+23003038 MsG0680031601.01.T01:CDS
GGAAAAGTTGCAGATTAAGG+TGG 0.645857 6:+23002029 MsG0680031601.01.T01:CDS
AGAATTTGCAGTGCAATGAG+AGG 0.669530 6:+23003000 MsG0680031601.01.T01:CDS
TGAAGGGTTAGAAATCCCAA+AGG 0.675804 6:-23001305 None:intergenic
GGTTGTTCTCAAGGTCACAT+GGG 0.692971 6:+23003021 MsG0680031601.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTGAATATTTTCAAA+AGG + Chr6:23001709-23001728 MsG0680031601.01.T01:intron 10.0%
!!! TTTTTGTGTTTTATTTTTTT+AGG + Chr6:23002931-23002950 MsG0680031601.01.T01:intron 10.0%
!!! AAAAATAATAAAGGGAATTT+TGG + Chr6:23001355-23001374 MsG0680031601.01.T01:CDS 15.0%
!!! AAAATAATAAAGGGAATTTT+GGG + Chr6:23001356-23001375 MsG0680031601.01.T01:CDS 15.0%
!!! TCATTTGTAATATTTTTTGA+AGG + Chr6:23001954-23001973 MsG0680031601.01.T01:intron 15.0%
!! AATTTATTTAATTAGTGCTC+CGG + Chr6:23002768-23002787 MsG0680031601.01.T01:intron 20.0%
!! CAAATATAGTAGTATTACAT+TGG + Chr6:23002498-23002517 MsG0680031601.01.T01:intron 20.0%
!! CAATGTAATACTACTATATT+TGG - Chr6:23002500-23002519 None:intergenic 20.0%
!! TTAATTACCATTATCACAAA+AGG - Chr6:23002007-23002026 None:intergenic 20.0%
!!! AAAAAACTTGTTGATGAATT+TGG + Chr6:23001490-23001509 MsG0680031601.01.T01:CDS 20.0%
!!! AAATAATAAAGGGAATTTTG+GGG + Chr6:23001357-23001376 MsG0680031601.01.T01:CDS 20.0%
!!! AACCTTTAAATTGCTTTTTT+AGG + Chr6:23002843-23002862 MsG0680031601.01.T01:intron 20.0%
!!! AATTTGTTATGTTCTAGTTT+AGG - Chr6:23001570-23001589 None:intergenic 20.0%
!!! ATGAACAATGAATCAATTTT+GGG + Chr6:23003123-23003142 MsG0680031601.01.T01:CDS 20.0%
!!! CAAATTTTGAAGCAATTTAA+AGG - Chr6:23002706-23002725 None:intergenic 20.0%
!!! TTGTGATAATGGTAATTAAA+GGG + Chr6:23002008-23002027 MsG0680031601.01.T01:intron 20.0%
!!! TTTGTGATAATGGTAATTAA+AGG + Chr6:23002007-23002026 MsG0680031601.01.T01:intron 20.0%
! AAACACAAAAATCAACAACA+TGG - Chr6:23002922-23002941 None:intergenic 25.0%
! AAATGTGAGAGATAAGATTA+AGG + Chr6:23003155-23003174 MsG0680031601.01.T01:CDS 25.0%
! AAGGAAGAAAATGAGAAATT+TGG + Chr6:23001412-23001431 MsG0680031601.01.T01:CDS 25.0%
! AGCATGGTCAAAAATAATAA+AGG + Chr6:23001346-23001365 MsG0680031601.01.T01:CDS 25.0%
! AGCCTAAAAAAGCAATTTAA+AGG - Chr6:23002848-23002867 None:intergenic 25.0%
! AGGAAGAAAATGAGAAATTT+GGG + Chr6:23001413-23001432 MsG0680031601.01.T01:CDS 25.0%
! ATTATAATCGCTGTTAAATG+CGG - Chr6:23002677-23002696 None:intergenic 25.0%
! ATTTATTTAATTAGTGCTCC+GGG + Chr6:23002769-23002788 MsG0680031601.01.T01:intron 25.0%
! GCATGGTCAAAAATAATAAA+GGG + Chr6:23001347-23001366 MsG0680031601.01.T01:CDS 25.0%
! TGAGTTTGAATATGAATGAT+TGG - Chr6:23003070-23003089 None:intergenic 25.0%
! TTGAATATGAATGATTGGAT+TGG - Chr6:23003065-23003084 None:intergenic 25.0%
!! CATGAACAATGAATCAATTT+TGG + Chr6:23003122-23003141 MsG0680031601.01.T01:CDS 25.0%
AAAGCTAACATTTCAAATCC+AGG + Chr6:23002261-23002280 MsG0680031601.01.T01:intron 30.0%
AACATTGCTGAACAAATACA+TGG + Chr6:23001529-23001548 MsG0680031601.01.T01:CDS 30.0%
AACTTGAACAATGATGGTTA+TGG + Chr6:23001172-23001191 MsG0680031601.01.T01:CDS 30.0%
AGGCTTACTTATAGAAAGAA+AGG + Chr6:23002951-23002970 MsG0680031601.01.T01:intron 30.0%
ATACACTTCATGGTAACAAA+TGG + Chr6:23002125-23002144 MsG0680031601.01.T01:intron 30.0%
ATAGAAAGAAAGGTAGAAGT+AGG + Chr6:23002961-23002980 MsG0680031601.01.T01:CDS 30.0%
CATACTCATTTGTGAATCAT+AGG - Chr6:23002394-23002413 None:intergenic 30.0%
CATTTGATAAGCTAAAGTGA+AGG - Chr6:23002425-23002444 None:intergenic 30.0%
GAAAGTTCAAAATACCCTTT+GGG + Chr6:23001290-23001309 MsG0680031601.01.T01:CDS 30.0%
GTATTACATTGGAAATTGTG+CGG + Chr6:23002509-23002528 MsG0680031601.01.T01:intron 30.0%
GTTGAGTTAGATTCATCAAA+CGG - Chr6:23002324-23002343 None:intergenic 30.0%
TAGAAAGAAAGGTAGAAGTA+GGG + Chr6:23002962-23002981 MsG0680031601.01.T01:CDS 30.0%
TAGCTTTCTCTTTCTTGAAA+TGG - Chr6:23002248-23002267 None:intergenic 30.0%
TATATTTATAACCATGTCCC+CGG - Chr6:23002790-23002809 None:intergenic 30.0%
TCACTAAACTTGAACAATGA+TGG + Chr6:23001166-23001185 MsG0680031601.01.T01:CDS 30.0%
TGAACAAATACATGGAAGAT+CGG + Chr6:23001537-23001556 MsG0680031601.01.T01:CDS 30.0%
TTTATTTAATTAGTGCTCCG+GGG + Chr6:23002770-23002789 MsG0680031601.01.T01:intron 30.0%
! GAATCATTTTCATGTGATCA+TGG + Chr6:23002195-23002214 MsG0680031601.01.T01:intron 30.0%
! TGAGTTTCCTTTTGTGATAA+TGG + Chr6:23001997-23002016 MsG0680031601.01.T01:intron 30.0%
! TTACTCTTTTTGCACTTTTG+TGG - Chr6:23002541-23002560 None:intergenic 30.0%
!! GTGTCATTTATTGACTTTCT+TGG + Chr6:23003177-23003196 MsG0680031601.01.T01:CDS 30.0%
!! TATATCGATTCTAACGCAAT+TGG + Chr6:23001808-23001827 MsG0680031601.01.T01:intron 30.0%
!! TTTATTGACTTTCTTGGAGT+AGG + Chr6:23003183-23003202 MsG0680031601.01.T01:CDS 30.0%
AAATGACAGCACAACTTCAA+TGG - Chr6:23002815-23002834 None:intergenic 35.0%
AAGGGAAAAGTTGCAGATTA+AGG + Chr6:23002026-23002045 MsG0680031601.01.T01:intron 35.0%
AGAAAGAAAGGTAGAAGTAG+GGG + Chr6:23002963-23002982 MsG0680031601.01.T01:CDS 35.0%
ATGAATTTGGTCCTCACAAT+TGG + Chr6:23001503-23001522 MsG0680031601.01.T01:CDS 35.0%
ATGACTCAAATATAGGTCTG+CGG + Chr6:23002623-23002642 MsG0680031601.01.T01:intron 35.0%
CACATTTGCAAATTCTTTCC+TGG + Chr6:23002151-23002170 MsG0680031601.01.T01:intron 35.0%
CTAGCTACATTATTCTTTCC+TGG - Chr6:23002282-23002301 None:intergenic 35.0%
CTTGTCAATGAAAGTTGACA+AGG + Chr6:23001393-23001412 MsG0680031601.01.T01:CDS 35.0%
GAACAAATACATGGAAGATC+GGG + Chr6:23001538-23001557 MsG0680031601.01.T01:CDS 35.0%
GCAACTTCAATTGAATATCG+TGG - Chr6:23002649-23002668 None:intergenic 35.0%
GGAAAGTTCAAAATACCCTT+TGG + Chr6:23001289-23001308 MsG0680031601.01.T01:CDS 35.0%
GGTTAGCATGACTCAAATAT+AGG + Chr6:23002616-23002635 MsG0680031601.01.T01:intron 35.0%
TCATTTGTGAATCATAGGCT+TGG - Chr6:23002389-23002408 None:intergenic 35.0%
TGATGGTTATGGTTTCTCTT+TGG + Chr6:23001183-23001202 MsG0680031601.01.T01:CDS 35.0%
TTAAGCTTAGCATCTTCAGA+TGG - Chr6:23001472-23001491 None:intergenic 35.0%
TTACCAATATGACCCAAAAG+AGG - Chr6:23001259-23001278 None:intergenic 35.0%
TTTGCAAATTCTTTCCTGGA+AGG + Chr6:23002155-23002174 MsG0680031601.01.T01:intron 35.0%
! ATTTTCATGTGATCATGGCT+AGG + Chr6:23002200-23002219 MsG0680031601.01.T01:intron 35.0%
! CATATTGGTAACAATGAGTC+TGG + Chr6:23001268-23001287 MsG0680031601.01.T01:CDS 35.0%
! TAGGCTTGGTAATTAAGCAA+AGG - Chr6:23002375-23002394 None:intergenic 35.0%
! TCATCTTCTACTCCTCTTTT+GGG + Chr6:23001244-23001263 MsG0680031601.01.T01:CDS 35.0%
! TTCATCAAACGGATTTGTGA+TGG - Chr6:23002313-23002332 None:intergenic 35.0%
! TTCATCTTCTACTCCTCTTT+TGG + Chr6:23001243-23001262 MsG0680031601.01.T01:CDS 35.0%
! TTTTTGACCATGCTCTCATT+AGG - Chr6:23001340-23001359 None:intergenic 35.0%
!! AAAGCTGCTCTATTGATTCT+AGG - Chr6:23002070-23002089 None:intergenic 35.0%
AACACCAAGCTTTCCATTAG+AGG + Chr6:23001436-23001455 MsG0680031601.01.T01:CDS 40.0%
AGAATTTGCAGTGCAATGAG+AGG + Chr6:23003000-23003019 MsG0680031601.01.T01:CDS 40.0%
AGAGGAGTAGAAGATGAAGA+AGG - Chr6:23001241-23001260 None:intergenic 40.0%
AGCTTTCCATTAGAGGTCAT+TGG + Chr6:23001443-23001462 MsG0680031601.01.T01:CDS 40.0%
ATGACCTCTAATGGAAAGCT+TGG - Chr6:23001443-23001462 None:intergenic 40.0%
CATTAGGATGGATCACTGAA+GGG - Chr6:23001324-23001343 None:intergenic 40.0%
GAAATGCTAACTAGTGTCAC+AGG + Chr6:23002458-23002477 MsG0680031601.01.T01:intron 40.0%
GAAGGGTTAGAAATCCCAAA+GGG - Chr6:23001307-23001326 None:intergenic 40.0%
GCAATGTTGTTCCAATTGTG+AGG - Chr6:23001517-23001536 None:intergenic 40.0%
GGAAAAGTTGCAGATTAAGG+TGG + Chr6:23002029-23002048 MsG0680031601.01.T01:CDS 40.0%
GTAGAAGATGAAGAAGGAAG+TGG - Chr6:23001235-23001254 None:intergenic 40.0%
TCATTAGGATGGATCACTGA+AGG - Chr6:23001325-23001344 None:intergenic 40.0%
TCGATTCTAACGCAATTGGT+TGG + Chr6:23001812-23001831 MsG0680031601.01.T01:intron 40.0%
TCTCATTGCACTGCAAATTC+TGG - Chr6:23003001-23003020 None:intergenic 40.0%
TGAAGGGTTAGAAATCCCAA+AGG - Chr6:23001308-23001327 None:intergenic 40.0%
TTGCATGCTCATACACTTCA+TGG + Chr6:23002115-23002134 MsG0680031601.01.T01:CDS 40.0%
! AGCTTTTAGTGAGGAAGAAG+AGG + Chr6:23002084-23002103 MsG0680031601.01.T01:CDS 40.0%
! CAATAGAGCAGCTTTTAGTG+AGG + Chr6:23002075-23002094 MsG0680031601.01.T01:CDS 40.0%
!! ACTCCTCTTTTGGGTCATAT+TGG + Chr6:23001253-23001272 MsG0680031601.01.T01:CDS 40.0%
AAGAAGGAAGTGGAGATGGA+AGG - Chr6:23001225-23001244 None:intergenic 45.0%
AAGGTAGAAGTAGGGGACTA+TGG + Chr6:23002970-23002989 MsG0680031601.01.T01:CDS 45.0%
AGAAGGAAGTGGAGATGGAA+GGG - Chr6:23001224-23001243 None:intergenic 45.0%
AGGTTGTTCTCAAGGTCACA+TGG + Chr6:23003020-23003039 MsG0680031601.01.T01:CDS 45.0%
CATGGGAAAGCTTAAGAGTG+TGG + Chr6:23003038-23003057 MsG0680031601.01.T01:CDS 45.0%
CCAATCTCAACCTCACTACT+AGG + Chr6:23002349-23002368 MsG0680031601.01.T01:intron 45.0%
CCTAGTAGTGAGGTTGAGAT+TGG - Chr6:23002352-23002371 None:intergenic 45.0%
GATCATGGCTAGGAAGCAAA+GGG + Chr6:23002210-23002229 MsG0680031601.01.T01:intron 45.0%
GATCCATCCTAATGAGAGCA+TGG + Chr6:23001330-23001349 MsG0680031601.01.T01:CDS 45.0%
GATGAAGAAGGAAGTGGAGA+TGG - Chr6:23001229-23001248 None:intergenic 45.0%
GCAATGAGAGGTTGTTCTCA+AGG + Chr6:23003012-23003031 MsG0680031601.01.T01:CDS 45.0%
GGTTGTTCTCAAGGTCACAT+GGG + Chr6:23003021-23003040 MsG0680031601.01.T01:CDS 45.0%
TAAGCAAAGGCCTAGTAGTG+AGG - Chr6:23002362-23002381 None:intergenic 45.0%
TGACCATGCTCTCATTAGGA+TGG - Chr6:23001336-23001355 None:intergenic 45.0%
TGATCATGGCTAGGAAGCAA+AGG + Chr6:23002209-23002228 MsG0680031601.01.T01:intron 45.0%
TGGTCTCCAATGACCTCTAA+TGG - Chr6:23001452-23001471 None:intergenic 45.0%
ACTTCTTCAACCAGTGCTCC+GGG + Chr6:23002587-23002606 MsG0680031601.01.T01:intron 50.0%
AGTGCATTGTCTGTCCTTCC+AGG - Chr6:23002172-23002191 None:intergenic 50.0%
GACTTCTTCAACCAGTGCTC+CGG + Chr6:23002586-23002605 MsG0680031601.01.T01:intron 50.0%
TAATTAGTGCTCCGGGGACA+TGG + Chr6:23002776-23002795 MsG0680031601.01.T01:intron 50.0%
CTTCTTCAACCAGTGCTCCG+GGG + Chr6:23002588-23002607 MsG0680031601.01.T01:intron 55.0%
GTCATGCTAACCAGTGTCCC+CGG - Chr6:23002608-23002627 None:intergenic 55.0%
AACCAGTGCTCCGGGGACAC+TGG + Chr6:23002595-23002614 MsG0680031601.01.T01:intron 65.0%
AACCAGTGTCCCCGGAGCAC+TGG - Chr6:23002600-23002619 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 23001160 23003215 23001160 ID=MsG0680031601.01;Name=MsG0680031601.01
Chr6 mRNA 23001160 23003215 23001160 ID=MsG0680031601.01.T01;Parent=MsG0680031601.01;Name=MsG0680031601.01.T01;_AED=0.21;_eAED=0.21;_QI=0|0|0|1|1|1|3|0|256
Chr6 exon 23001160 23001559 23001160 ID=MsG0680031601.01.T01:exon:14429;Parent=MsG0680031601.01.T01
Chr6 exon 23002029 23002136 23002029 ID=MsG0680031601.01.T01:exon:14430;Parent=MsG0680031601.01.T01
Chr6 exon 23002953 23003215 23002953 ID=MsG0680031601.01.T01:exon:14431;Parent=MsG0680031601.01.T01
Chr6 CDS 23001160 23001559 23001160 ID=MsG0680031601.01.T01:cds;Parent=MsG0680031601.01.T01
Chr6 CDS 23002029 23002136 23002029 ID=MsG0680031601.01.T01:cds;Parent=MsG0680031601.01.T01
Chr6 CDS 23002953 23003215 23002953 ID=MsG0680031601.01.T01:cds;Parent=MsG0680031601.01.T01
Gene Sequence

>MsG0680031601.01.T01

ATGAGTTCACTAAACTTGAACAATGATGGTTATGGTTTCTCTTTGGACTTGAATATTTCTTCCCTTCCATCTCCACTTCCTTCTTCATCTTCTACTCCTCTTTTGGGTCATATTGGTAACAATGAGTCTGGAAAGTTCAAAATACCCTTTGGGATTTCTAACCCTTCAGTGATCCATCCTAATGAGAGCATGGTCAAAAATAATAAAGGGAATTTTGGGGATGAGAGAAAGAACTTGTCAATGAAAGTTGACAAGGAAGAAAATGAGAAATTTGGGAACACCAAGCTTTCCATTAGAGGTCATTGGAGACCATCTGAAGATGCTAAGCTTAAAAAACTTGTTGATGAATTTGGTCCTCACAATTGGAACAACATTGCTGAACAAATACATGGAAGATCGGGGAAAAGTTGCAGATTAAGGTGGTGTAATCAGCTAGATCCTAGAATCAATAGAGCAGCTTTTAGTGAGGAAGAAGAGGAGAAGCTTTTGCATGCTCATACACTTCATGGCTTACTTATAGAAAGAAAGGTAGAAGTAGGGGACTATGGTTTTGCCAGAATTTGCAGTGCAATGAGAGGTTGTTCTCAAGGTCACATGGGAAAGCTTAAGAGTGTGGACCAATCCAATCATTCATATTCAAACTCAGAAGTTTCAGTATCTGAGTCAGTTGCAACAAACATGAACAATGAATCAATTTTGGGAGAAAGTGAAAATGTGAGAGATAAGATTAAGGTGTCATTTATTGACTTTCTTGGAGTAGGTGATACATAG
Protein sequence

>MsG0680031601.01.T01

MSSLNLNNDGYGFSLDLNISSLPSPLPSSSSTPLLGHIGNNESGKFKIPFGISNPSVIHPNESMVKNNKGNFGDERKNLSMKVDKEENEKFGNTKLSIRGHWRPSEDAKLKKLVDEFGPHNWNNIAEQIHGRSGKSCRLRWCNQLDPRINRAAFSEEEEEKLLHAHTLHGLLIERKVEVGDYGFARICSAMRGCSQGHMGKLKSVDQSNHSYSNSEVSVSESVATNMNNESILGESENVRDKIKVSFIDFLGVGDT*