AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031614.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031614.01.T01 MTR_6g027460 91.645 383 17 3 1 383 1 368 0.0 680
MsG0680031614.01.T01 MTR_6g027450 73.569 367 59 6 1 367 1 329 0.0 522
MsG0680031614.01.T01 MTR_7g086780 50.000 380 137 10 15 383 10 347 2.90e-113 337
MsG0680031614.01.T01 MTR_7g010950 46.392 194 75 6 56 246 94 261 2.22e-43 157
MsG0680031614.01.T01 MTR_6g012450 32.951 349 165 10 55 383 96 395 7.22e-43 156
MsG0680031614.01.T01 MTR_4g082060 33.951 324 145 12 9 289 4 301 4.92e-40 148
MsG0680031614.01.T01 MTR_3g435480 35.616 292 127 11 23 281 77 340 8.54e-40 147
MsG0680031614.01.T01 MTR_8g044220 31.250 320 137 10 10 289 10 286 1.74e-36 138
MsG0680031614.01.T01 MTR_5g041420 53.509 114 44 2 55 162 99 209 3.12e-34 129
MsG0680031614.01.T01 MTR_5g041530 52.294 109 49 1 54 162 100 205 1.92e-30 120
MsG0680031614.01.T01 MTR_2g016030 77.419 62 14 0 89 150 39 100 3.16e-30 114
MsG0680031614.01.T01 MTR_5g041380 62.791 86 27 2 65 150 100 180 2.13e-29 117
MsG0680031614.01.T01 MTR_5g041400 57.778 90 32 2 61 150 89 172 9.00e-29 115
MsG0680031614.01.T01 MTR_8g068210 47.872 94 36 2 59 152 7 87 4.20e-24 102
MsG0680031614.01.T01 MTR_8g027295 60.317 63 25 0 92 154 19 81 1.25e-23 99.4
MsG0680031614.01.T01 MTR_4g022370 60.656 61 24 0 90 150 75 135 1.79e-23 100
MsG0680031614.01.T01 MTR_4g088580 44.762 105 48 2 56 152 8 110 2.18e-23 100
MsG0680031614.01.T01 MTR_4g461080 55.844 77 33 1 74 149 5 81 2.34e-23 98.6
MsG0680031614.01.T01 MTR_2g093220 55.263 76 33 1 76 150 18 93 4.91e-23 98.2
MsG0680031614.01.T01 MTR_3g091820 54.930 71 32 0 81 151 27 97 5.51e-23 98.2
MsG0680031614.01.T01 MTR_8g479350 52.703 74 35 0 97 170 68 141 8.60e-23 98.6
MsG0680031614.01.T01 MTR_2g096740 56.716 67 29 0 84 150 64 130 1.25e-22 97.4
MsG0680031614.01.T01 MTR_2g096740 56.716 67 29 0 84 150 49 115 1.38e-22 96.7
MsG0680031614.01.T01 MTR_4g063780 61.667 60 23 0 90 149 68 127 1.75e-22 97.4
MsG0680031614.01.T01 MTR_8g079060 42.623 122 63 3 97 218 29 143 2.38e-22 94.7
MsG0680031614.01.T01 MTR_4g089095 48.485 99 35 2 69 152 7 104 6.98e-22 95.1
MsG0680031614.01.T01 MTR_2g013370 67.241 58 18 1 95 152 44 100 1.43e-21 93.6
MsG0680031614.01.T01 MTR_2g059540 69.388 49 15 0 97 145 31 79 1.77e-21 91.7
MsG0680031614.01.T01 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.05e-21 93.2
MsG0680031614.01.T01 MTR_2g014060 54.688 64 29 0 85 148 13 76 2.17e-21 94.4
MsG0680031614.01.T01 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.33e-21 93.6
MsG0680031614.01.T01 MTR_4g089095 69.643 56 16 1 97 152 16 70 2.44e-21 92.8
MsG0680031614.01.T01 MTR_7g059400 53.521 71 31 1 82 150 59 129 2.75e-21 94.4
MsG0680031614.01.T01 MTR_8g015840 65.385 52 18 0 97 148 20 71 3.27e-21 91.3
MsG0680031614.01.T01 MTR_4g109980 64.151 53 19 0 95 147 38 90 5.27e-21 92.8
MsG0680031614.01.T01 MTR_7g024670 67.308 52 17 0 98 149 78 129 6.91e-21 93.6
MsG0680031614.01.T01 MTR_3g090430 54.167 72 31 1 81 150 33 104 2.80e-20 91.3
MsG0680031614.01.T01 MTR_5g031440 60.000 55 22 0 93 147 39 93 2.97e-20 91.3
MsG0680031614.01.T01 MTR_2g014170 67.347 49 16 0 97 145 45 93 6.71e-20 89.7
MsG0680031614.01.T01 MTR_3g077750 56.452 62 26 1 87 147 50 111 1.19e-19 89.4
MsG0680031614.01.T01 MTR_1g056810 65.385 52 18 0 99 150 14 65 1.53e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031614.01.T01 AT3G47500 56.667 120 46 3 53 172 71 184 2.27e-39 146
MsG0680031614.01.T01 AT5G39660 44.828 174 61 6 63 221 92 245 9.95e-36 136
MsG0680031614.01.T01 AT5G39660 44.828 174 61 6 63 221 92 245 9.95e-36 136
MsG0680031614.01.T01 AT5G39660 44.828 174 61 6 63 221 92 245 9.95e-36 136
MsG0680031614.01.T01 AT5G62430 52.137 117 49 2 64 178 26 137 6.11e-35 130
MsG0680031614.01.T01 AT2G34140 58.065 93 26 1 71 150 19 111 8.86e-34 124
MsG0680031614.01.T01 AT1G26790 55.963 109 44 2 55 159 67 175 9.43e-34 129
MsG0680031614.01.T01 AT1G29160 78.333 60 13 0 91 150 56 115 3.04e-31 117
MsG0680031614.01.T01 AT1G69570 45.385 130 43 2 85 213 120 222 3.48e-31 122
MsG0680031614.01.T01 AT3G55370 57.143 70 30 0 93 162 72 141 1.72e-24 102
MsG0680031614.01.T01 AT3G55370 57.143 70 30 0 93 162 72 141 2.00e-24 103
MsG0680031614.01.T01 AT3G55370 57.143 70 30 0 93 162 117 186 4.38e-24 102
MsG0680031614.01.T01 AT2G28810 40.000 100 57 1 52 148 29 128 3.95e-23 99.0
MsG0680031614.01.T01 AT2G28810 40.000 100 57 1 52 148 46 145 4.67e-23 99.4
MsG0680031614.01.T01 AT5G60200 61.667 60 23 0 91 150 49 108 6.75e-23 97.1
MsG0680031614.01.T01 AT3G21270 69.811 53 16 0 97 149 29 81 1.01e-22 95.1
MsG0680031614.01.T01 AT2G37590 62.069 58 22 0 93 150 85 142 1.45e-22 97.8
MsG0680031614.01.T01 AT3G45610 45.055 91 42 2 93 175 36 126 2.18e-22 95.5
MsG0680031614.01.T01 AT1G51700 52.857 70 31 1 79 148 17 84 3.74e-22 93.6
MsG0680031614.01.T01 AT1G28310 64.286 56 20 0 97 152 27 82 1.13e-21 94.7
MsG0680031614.01.T01 AT4G24060 56.923 65 28 0 86 150 42 106 1.33e-21 95.1
MsG0680031614.01.T01 AT3G61850 61.017 59 23 0 92 150 57 115 1.35e-21 94.0
MsG0680031614.01.T01 AT3G61850 61.017 59 23 0 92 150 57 115 1.35e-21 94.0
MsG0680031614.01.T01 AT1G28310 64.286 56 20 0 97 152 51 106 1.36e-21 95.1
MsG0680031614.01.T01 AT3G61850 61.017 59 23 0 92 150 69 127 1.57e-21 94.0
MsG0680031614.01.T01 AT5G60850 66.667 54 18 0 97 150 51 104 1.92e-21 94.0
MsG0680031614.01.T01 AT3G61850 61.017 59 23 0 92 150 57 115 2.21e-21 94.0
MsG0680031614.01.T01 AT1G07640 66.038 53 18 0 97 149 21 73 2.26e-21 93.2
MsG0680031614.01.T01 AT2G28510 66.071 56 19 0 99 154 50 105 2.38e-21 93.6
MsG0680031614.01.T01 AT3G61850 61.017 59 23 0 92 150 69 127 2.40e-21 94.0
MsG0680031614.01.T01 AT3G50410 66.038 53 18 0 97 149 30 82 2.40e-21 92.8
MsG0680031614.01.T01 AT5G02460 59.016 61 25 0 90 150 88 148 3.65e-21 94.7
MsG0680031614.01.T01 AT1G07640 66.038 53 18 0 97 149 77 129 3.74e-21 93.6
MsG0680031614.01.T01 AT5G66940 53.425 73 27 1 84 156 26 91 3.89e-21 91.3
MsG0680031614.01.T01 AT1G07640 66.038 53 18 0 97 149 85 137 4.19e-21 93.6
MsG0680031614.01.T01 AT5G65590 64.815 54 19 0 97 150 41 94 5.64e-21 92.8
MsG0680031614.01.T01 AT4G00940 52.778 72 30 1 85 152 52 123 1.67e-20 91.3
MsG0680031614.01.T01 AT4G00940 52.778 72 30 1 85 152 52 123 1.67e-20 91.3
MsG0680031614.01.T01 AT4G00940 52.778 72 30 1 85 152 52 123 1.67e-20 91.3
MsG0680031614.01.T01 AT1G21340 42.553 94 53 1 99 191 40 133 4.99e-20 89.0
MsG0680031614.01.T01 AT5G62940 39.450 109 50 2 55 147 15 123 6.30e-20 90.9
MsG0680031614.01.T01 AT4G21050 47.436 78 39 1 77 152 2 79 7.16e-20 87.4
MsG0680031614.01.T01 AT4G38000 49.367 79 38 2 96 172 40 118 9.61e-20 88.2
MsG0680031614.01.T01 AT3G52440 63.462 52 19 0 99 150 27 78 2.07e-19 87.0
MsG0680031614.01.T01 AT1G64620 53.333 60 28 0 86 145 38 97 2.32e-19 89.0
MsG0680031614.01.T01 AT1G47655 65.306 49 17 0 97 145 29 77 2.87e-19 85.9
MsG0680031614.01.T01 AT3G52440 63.462 52 19 0 99 150 46 97 2.99e-19 87.0
MsG0680031614.01.T01 AT2G46590 58.491 53 22 0 92 144 63 115 1.15e-18 87.0
MsG0680031614.01.T01 AT2G46590 58.491 53 22 0 92 144 75 127 1.39e-18 86.7
MsG0680031614.01.T01 AT4G21040 48.611 72 37 0 99 170 27 98 8.46e-16 76.6
MsG0680031614.01.T01 AT4G21080 50.877 57 28 0 99 155 27 83 1.12e-14 73.6

Find 78 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGTATTGTCCTAAAATT+TGG 0.197738 6:-23225805 MsG0680031614.01.T01:CDS
CAATCTTGTCTGGTTTCTTT+AGG 0.212759 6:+23226127 None:intergenic
TAGAAACCAACGACTCTTCT+AGG 0.239536 6:-23225882 MsG0680031614.01.T01:CDS
TTGGAACAATGTTACTTCTA+TGG 0.251370 6:-23225539 MsG0680031614.01.T01:CDS
GGTAACAATTGTAGAGAAAA+TGG 0.277928 6:-23225703 MsG0680031614.01.T01:CDS
GGTGAGTTCTTCTCAGATTC+AGG 0.283209 6:-23227269 MsG0680031614.01.T01:CDS
AAGATTAAGCATTGAACCTT+TGG 0.298547 6:+23225756 None:intergenic
TCTTCTAGTTTCATGCTCTT+TGG 0.299226 6:-23227304 MsG0680031614.01.T01:CDS
TTTAGGTGTTCAAATGATAA+TGG 0.318424 6:-23225823 MsG0680031614.01.T01:CDS
GTCGGAGTTGTCACAGATAT+TGG 0.320960 6:-23226023 MsG0680031614.01.T01:CDS
TTGTTCCAACTTTGATTCAT+TGG 0.340788 6:+23225553 None:intergenic
ATTTACCATGGTTGTAGAAT+TGG 0.341653 6:+23227246 None:intergenic
CCTTTGTATGGTAGTGGAAA+TGG 0.370217 6:-23225370 MsG0680031614.01.T01:CDS
ATGCAGATGTGTAACCCTTA+TGG 0.380909 6:-23225505 MsG0680031614.01.T01:CDS
AGAGTCGTTGGTTTCTAAAC+TGG 0.381811 6:+23225888 None:intergenic
GAGCACTGTGGAAGATAATA+TGG 0.382590 6:-23226178 MsG0680031614.01.T01:CDS
GGACATTGAACAATCTTGTC+TGG 0.411323 6:+23226117 None:intergenic
ACAAATTGCAAGCCAATGTT+TGG 0.418330 6:+23225418 None:intergenic
GCTCTTTGGTAAGAAGATTC+CGG 0.421687 6:-23227290 MsG0680031614.01.T01:CDS
TGCAGTAACTCAAAGCATAA+AGG 0.422749 6:-23226279 MsG0680031614.01.T01:CDS
AGGTTTAGACCTATTCTGTT+CGG 0.445629 6:+23225609 None:intergenic
AATGCAACCATTTGAACCTA+TGG 0.446070 6:+23225339 None:intergenic
GTGTCACGCGCCGAGGGTTG+CGG 0.447216 6:-23225458 MsG0680031614.01.T01:CDS
ACTAGTAATAGTTGTATCTC+AGG 0.449615 6:-23225298 MsG0680031614.01.T01:CDS
AATTTGTTCCTGCATCAAAC+AGG 0.461385 6:-23225402 MsG0680031614.01.T01:CDS
AGAAATGTTGCTGTCGGTGC+AGG 0.467099 6:-23225982 MsG0680031614.01.T01:CDS
GTTCCATTCCTGTTTGATGC+AGG 0.467432 6:+23225394 None:intergenic
AGAGCTACCCTGCATCTTCA+TGG 0.473280 6:-23225582 MsG0680031614.01.T01:CDS
TATTGTCCTAAAATTTGGTC+GGG 0.484036 6:-23225800 MsG0680031614.01.T01:CDS
TATTCGAATCCGAACAGAAT+AGG 0.487381 6:-23225618 MsG0680031614.01.T01:CDS
GGAATTATCCATGAAGATGC+AGG 0.489315 6:+23225574 None:intergenic
GAAGTAGGATCAATGCCATA+AGG 0.489322 6:+23225490 None:intergenic
GGTAATGGCACACCAATCCT+TGG 0.490161 6:-23225277 MsG0680031614.01.T01:CDS
AATAGTTGTATCTCAGGTAA+TGG 0.494093 6:-23225292 MsG0680031614.01.T01:CDS
GGAACACCTTTGTATGGTAG+TGG 0.497692 6:-23225376 MsG0680031614.01.T01:CDS
TTGCTGTCGGTGCAGGGAGA+CGG 0.499466 6:-23225975 MsG0680031614.01.T01:CDS
TAATTCCAATGAATCAAAGT+TGG 0.500347 6:-23225558 MsG0680031614.01.T01:CDS
TGCAGTGGTGTCACGCGCCG+AGG 0.505833 6:-23225465 MsG0680031614.01.T01:CDS
ATTTCTCATTGTTCCACCGT+TGG 0.510921 6:+23225999 None:intergenic
GCAGTGGTGTCACGCGCCGA+GGG 0.511659 6:-23225464 MsG0680031614.01.T01:CDS
AGGAATGGAACACCTTTGTA+TGG 0.533609 6:-23225382 MsG0680031614.01.T01:CDS
TTGGTACCTAGAAGAGTCGT+TGG 0.535519 6:+23225876 None:intergenic
GTATTGTCCTAAAATTTGGT+CGG 0.547769 6:-23225801 MsG0680031614.01.T01:CDS
ATTCAACATTTGAGCCATGT+TGG 0.548791 6:+23225857 None:intergenic
AATGTGTCCATAGGTTCAAA+TGG 0.549266 6:-23225346 MsG0680031614.01.T01:CDS
GTTCCTGCATCAAACAGGAA+TGG 0.554691 6:-23225397 MsG0680031614.01.T01:CDS
TGTGTATCGTCTGTGACAAA+CGG 0.557160 6:-23225661 MsG0680031614.01.T01:CDS
ACCTTTGGATTCATCGAGAG+AGG 0.559535 6:+23225771 None:intergenic
AAGTAGGATCAATGCCATAA+GGG 0.561604 6:+23225491 None:intergenic
AAATGGAGAGGAAGAATCAT+CGG 0.568618 6:-23225686 MsG0680031614.01.T01:CDS
GGAAATGGAAATGTGTCCAT+AGG 0.578755 6:-23225355 MsG0680031614.01.T01:CDS
TTGGTTTCTAAACTGGCGGT+CGG 0.586129 6:+23225895 None:intergenic
TGACACCACTGCATTGAAGT+AGG 0.587068 6:+23225475 None:intergenic
ATCAACCTCGGCATTTCTGT+CGG 0.588083 6:-23226041 MsG0680031614.01.T01:CDS
ATGTTTGTAACCGCAACCCT+CGG 0.590311 6:+23225448 None:intergenic
TCAGGCCAATTCTACAACCA+TGG 0.595350 6:-23227251 MsG0680031614.01.T01:intron
CACATGATTGTAGTGTCCGA+TGG 0.602575 6:-23225922 MsG0680031614.01.T01:CDS
GTCGTTGGTTTCTAAACTGG+CGG 0.606694 6:+23225891 None:intergenic
GATGCAGGGTAGCTCTGCAT+AGG 0.608266 6:+23225589 None:intergenic
CCATTTCCACTACCATACAA+AGG 0.611279 6:+23225370 None:intergenic
CTCAAATGTTGAATCTGCTG+CGG 0.611992 6:-23225848 MsG0680031614.01.T01:CDS
GCCTCTCTCGATGAATCCAA+AGG 0.612065 6:-23225772 MsG0680031614.01.T01:CDS
TTACTAGTAGTAGATGAAGA+AGG 0.612171 6:+23225313 None:intergenic
GAATTATCCATGAAGATGCA+GGG 0.627674 6:+23225575 None:intergenic
CACAGATATTGGACCAACGG+TGG 0.629754 6:-23226012 MsG0680031614.01.T01:CDS
TGAATCTGAGAAGAACTCAC+CGG 0.630634 6:+23227271 None:intergenic
TGTCACAGATATTGGACCAA+CGG 0.638912 6:-23226015 MsG0680031614.01.T01:CDS
ACTGGCGGTCGGAATTCCAT+CGG 0.649455 6:+23225906 None:intergenic
TTGATCCTACTTCAATGCAG+TGG 0.649462 6:-23225480 MsG0680031614.01.T01:CDS
GTGACAAACGGTCATACTCG+AGG 0.662893 6:-23225649 MsG0680031614.01.T01:CDS
GAAATGTTGCTGTCGGTGCA+GGG 0.663844 6:-23225981 MsG0680031614.01.T01:CDS
CAACTCCGACAGAAATGCCG+AGG 0.680790 6:+23226036 None:intergenic
ACTACAACGTCAATCAACCT+CGG 0.682406 6:-23226053 MsG0680031614.01.T01:CDS
GACTCTTCTAGGTACCAACA+TGG 0.687547 6:-23225871 MsG0680031614.01.T01:CDS
ACAATGAGAAATGTTGCTGT+CGG 0.691886 6:-23225988 MsG0680031614.01.T01:CDS
CATCGGACACTACAATCATG+TGG 0.695881 6:+23225923 None:intergenic
CAATTGTAGAGAAAATGGAG+AGG 0.705347 6:-23225698 MsG0680031614.01.T01:CDS
AAAAGTGAACACGAGCACTG+TGG 0.732007 6:-23226190 MsG0680031614.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTTTTTTTTTGTTT+TGG - Chr6:23225646-23225665 MsG0680031614.01.T01:CDS 10.0%
!!! ACATTAAATTTGTTTAATTT+TGG - Chr6:23225860-23225879 MsG0680031614.01.T01:CDS 10.0%
!!! ATTTTTTATTTTTGTTGTAT+TGG - Chr6:23225460-23225479 MsG0680031614.01.T01:CDS 10.0%
!!! TTGTTTTTTTATAAGAAAAT+TGG - Chr6:23225769-23225788 MsG0680031614.01.T01:CDS 10.0%
!!! TTTTTTATTTTTGTTGTATT+GGG - Chr6:23225461-23225480 MsG0680031614.01.T01:CDS 10.0%
!! AAAAAAAAAACTCAAGAATA+TGG - Chr6:23225571-23225590 MsG0680031614.01.T01:CDS 15.0%
!! AATTTAAATGTTTCAAAGAT+TGG + Chr6:23225502-23225521 None:intergenic 15.0%
!! TAAAAAAACAACATAAACAA+TGG + Chr6:23225416-23225435 None:intergenic 15.0%
!!! TAAATTTGTTTAATTTTGGT+TGG - Chr6:23225864-23225883 MsG0680031614.01.T01:CDS 15.0%
!! ATAAAAAACCATAAAATTCG+AGG + Chr6:23226189-23226208 None:intergenic 20.0%
!! ATAAATATAACATGATTAGC+TGG - Chr6:23225948-23225967 MsG0680031614.01.T01:CDS 20.0%
!! ATGTCAATACATACATATAT+TGG - Chr6:23225696-23225715 MsG0680031614.01.T01:CDS 20.0%
!!! TTAAGAGTATTTGATCTATA+TGG - Chr6:23225523-23225542 MsG0680031614.01.T01:CDS 20.0%
! AAGTCATGAAGAATAAAGTT+TGG + Chr6:23226266-23226285 None:intergenic 25.0%
! AATGGTATTGTCCTAAAATT+TGG - Chr6:23226796-23226815 MsG0680031614.01.T01:intron 25.0%
! ACAACATAAACAATGGTTAT+TGG + Chr6:23225409-23225428 None:intergenic 25.0%
! CAACATAAACAATGGTTATT+GGG + Chr6:23225408-23225427 None:intergenic 25.0%
! GAATTTAAGAATTGAGTCTA+AGG - Chr6:23225720-23225739 MsG0680031614.01.T01:CDS 25.0%
! GATAAAATGAGTATTTACCA+TGG + Chr6:23225370-23225389 None:intergenic 25.0%
! TAATTCCAATGAATCAAAGT+TGG - Chr6:23227043-23227062 MsG0680031614.01.T01:intron 25.0%
! TGTGAAATAAACTGAAATTG+TGG - Chr6:23225971-23225990 MsG0680031614.01.T01:CDS 25.0%
! TTTAGGTGTTCAAATGATAA+TGG - Chr6:23226778-23226797 MsG0680031614.01.T01:intron 25.0%
!!! CAGATTAGTTTTAGTAACAT+AGG - Chr6:23225994-23226013 MsG0680031614.01.T01:CDS 25.0%
!!! TTATTTTTGTTGTATTGGGT+TGG - Chr6:23225465-23225484 MsG0680031614.01.T01:CDS 25.0%
AAAAACCATAAAATTCGAGG+TGG + Chr6:23226186-23226205 None:intergenic 30.0%
AAGAAAAATGTTGATGCAAG+TGG - Chr6:23226877-23226896 MsG0680031614.01.T01:intron 30.0%
AATAGTTGTATCTCAGGTAA+TGG - Chr6:23227309-23227328 MsG0680031614.01.T01:CDS 30.0%
ACTAGTAATAGTTGTATCTC+AGG - Chr6:23227303-23227322 MsG0680031614.01.T01:CDS 30.0%
ATTTACCATGGTTGTAGAAT+TGG + Chr6:23225358-23225377 None:intergenic 30.0%
GATCTGATTCATGTAAACAA+TGG - Chr6:23225593-23225612 MsG0680031614.01.T01:CDS 30.0%
GGTAACAATTGTAGAGAAAA+TGG - Chr6:23226898-23226917 MsG0680031614.01.T01:intron 30.0%
GTATTGTCCTAAAATTTGGT+CGG - Chr6:23226800-23226819 MsG0680031614.01.T01:intron 30.0%
TATTGTCCTAAAATTTGGTC+GGG - Chr6:23226801-23226820 MsG0680031614.01.T01:intron 30.0%
TTACTAGTAGTAGATGAAGA+AGG + Chr6:23227291-23227310 None:intergenic 30.0%
TTGTTCCAACTTTGATTCAT+TGG + Chr6:23227051-23227070 None:intergenic 30.0%
! AACATTTTCACACCAAACAT+TGG - Chr6:23227171-23227190 MsG0680031614.01.T01:intron 30.0%
! TTGCATCAACATTTTTCTTG+TGG + Chr6:23226876-23226895 None:intergenic 30.0%
! TTGGAACAATGTTACTTCTA+TGG - Chr6:23227062-23227081 MsG0680031614.01.T01:intron 30.0%
!! AAGATTAAGCATTGAACCTT+TGG + Chr6:23226848-23226867 None:intergenic 30.0%
!! TTCTCAATGTTTTGAGTATG+AGG + Chr6:23226358-23226377 None:intergenic 30.0%
AAATGGAGAGGAAGAATCAT+CGG - Chr6:23226915-23226934 MsG0680031614.01.T01:intron 35.0%
AAGTAGGATCAATGCCATAA+GGG + Chr6:23227113-23227132 None:intergenic 35.0%
AATGCAACCATTTGAACCTA+TGG + Chr6:23227265-23227284 None:intergenic 35.0%
AATGTGTCCATAGGTTCAAA+TGG - Chr6:23227255-23227274 MsG0680031614.01.T01:CDS 35.0%
ACAAATTGCAAGCCAATGTT+TGG + Chr6:23227186-23227205 None:intergenic 35.0%
ACAATGAGAAATGTTGCTGT+CGG - Chr6:23226613-23226632 MsG0680031614.01.T01:intron 35.0%
AGGTTTAGACCTATTCTGTT+CGG + Chr6:23226995-23227014 None:intergenic 35.0%
ATTCAACATTTGAGCCATGT+TGG + Chr6:23226747-23226766 None:intergenic 35.0%
CAATTGTAGAGAAAATGGAG+AGG - Chr6:23226903-23226922 MsG0680031614.01.T01:intron 35.0%
GAATTATCCATGAAGATGCA+GGG + Chr6:23227029-23227048 None:intergenic 35.0%
TATTCGAATCCGAACAGAAT+AGG - Chr6:23226983-23227002 MsG0680031614.01.T01:intron 35.0%
TGCAGTAACTCAAAGCATAA+AGG - Chr6:23226322-23226341 MsG0680031614.01.T01:intron 35.0%
! AATTTGTTCCTGCATCAAAC+AGG - Chr6:23227199-23227218 MsG0680031614.01.T01:intron 35.0%
! ATCTACCACCTCGAATTTTA+TGG - Chr6:23226178-23226197 MsG0680031614.01.T01:CDS 35.0%
! CAATCTTGTCTGGTTTCTTT+AGG + Chr6:23226477-23226496 None:intergenic 35.0%
! CATTTTCCCGACCAAATTTT+AGG + Chr6:23226810-23226829 None:intergenic 35.0%
!! TCTTCTAGTTTCATGCTCTT+TGG - Chr6:23225297-23225316 MsG0680031614.01.T01:CDS 35.0%
ACTACAACGTCAATCAACCT+CGG - Chr6:23226548-23226567 MsG0680031614.01.T01:intron 40.0%
AGGAATGGAACACCTTTGTA+TGG - Chr6:23227219-23227238 MsG0680031614.01.T01:intron 40.0%
ATGCAGATGTGTAACCCTTA+TGG - Chr6:23227096-23227115 MsG0680031614.01.T01:intron 40.0%
ATTTCTCATTGTTCCACCGT+TGG + Chr6:23226605-23226624 None:intergenic 40.0%
CCATTTCCACTACCATACAA+AGG + Chr6:23227234-23227253 None:intergenic 40.0%
CGCTGAGTTTGACAAAATCA+CGG - Chr6:23226046-23226065 MsG0680031614.01.T01:CDS 40.0%
CTCAAATGTTGAATCTGCTG+CGG - Chr6:23226753-23226772 MsG0680031614.01.T01:intron 40.0%
CTGGTAATTCCATAAACCAG+GGG + Chr6:23226026-23226045 None:intergenic 40.0%
GAAGTAGGATCAATGCCATA+AGG + Chr6:23227114-23227133 None:intergenic 40.0%
GCTCTTTGGTAAGAAGATTC+CGG - Chr6:23225311-23225330 MsG0680031614.01.T01:CDS 40.0%
GCTGGTAATTCCATAAACCA+GGG + Chr6:23226027-23226046 None:intergenic 40.0%
GGAAATGGAAATGTGTCCAT+AGG - Chr6:23227246-23227265 MsG0680031614.01.T01:intron 40.0%
GGAATTATCCATGAAGATGC+AGG + Chr6:23227030-23227049 None:intergenic 40.0%
GGACATTGAACAATCTTGTC+TGG + Chr6:23226487-23226506 None:intergenic 40.0%
TAGAAACCAACGACTCTTCT+AGG - Chr6:23226719-23226738 MsG0680031614.01.T01:intron 40.0%
TGAATCTGAGAAGAACTCAC+CGG + Chr6:23225333-23225352 None:intergenic 40.0%
TGTCACAGATATTGGACCAA+CGG - Chr6:23226586-23226605 MsG0680031614.01.T01:intron 40.0%
TGTGTATCGTCTGTGACAAA+CGG - Chr6:23226940-23226959 MsG0680031614.01.T01:intron 40.0%
TTGATCCTACTTCAATGCAG+TGG - Chr6:23227121-23227140 MsG0680031614.01.T01:intron 40.0%
! CATGTGTGTTTTCCAAGGAT+TGG + Chr6:23227339-23227358 None:intergenic 40.0%
! GAGCACTGTGGAAGATAATA+TGG - Chr6:23226423-23226442 MsG0680031614.01.T01:intron 40.0%
! GTGTCGAAACTTTTGCATCT+CGG + Chr6:23226508-23226527 None:intergenic 40.0%
! TTGAATCTGCTGCGGTTTTT+AGG - Chr6:23226761-23226780 MsG0680031614.01.T01:intron 40.0%
!! AGAGTCGTTGGTTTCTAAAC+TGG + Chr6:23226716-23226735 None:intergenic 40.0%
!! CCTTTGTATGGTAGTGGAAA+TGG - Chr6:23227231-23227250 MsG0680031614.01.T01:intron 40.0%
ACCTTTGGATTCATCGAGAG+AGG + Chr6:23226833-23226852 None:intergenic 45.0%
ATCAACCTCGGCATTTCTGT+CGG - Chr6:23226560-23226579 MsG0680031614.01.T01:intron 45.0%
ATGTTTGTAACCGCAACCCT+CGG + Chr6:23227156-23227175 None:intergenic 45.0%
CACATGATTGTAGTGTCCGA+TGG - Chr6:23226679-23226698 MsG0680031614.01.T01:intron 45.0%
CATCGGACACTACAATCATG+TGG + Chr6:23226681-23226700 None:intergenic 45.0%
CGCTGGTAATTCCATAAACC+AGG + Chr6:23226028-23226047 None:intergenic 45.0%
GACTCTTCTAGGTACCAACA+TGG - Chr6:23226730-23226749 MsG0680031614.01.T01:intron 45.0%
GGAACACCTTTGTATGGTAG+TGG - Chr6:23227225-23227244 MsG0680031614.01.T01:intron 45.0%
GGTGAGTTCTTCTCAGATTC+AGG - Chr6:23225332-23225351 MsG0680031614.01.T01:CDS 45.0%
GTCGGAGTTGTCACAGATAT+TGG - Chr6:23226578-23226597 MsG0680031614.01.T01:intron 45.0%
GTTAAGCTGTCTTGTGCAAG+TGG - Chr6:23225746-23225765 MsG0680031614.01.T01:CDS 45.0%
GTTCCATTCCTGTTTGATGC+AGG + Chr6:23227210-23227229 None:intergenic 45.0%
GTTCCTGCATCAAACAGGAA+TGG - Chr6:23227204-23227223 MsG0680031614.01.T01:intron 45.0%
TCAGGCCAATTCTACAACCA+TGG - Chr6:23225350-23225369 MsG0680031614.01.T01:CDS 45.0%
TGACACCACTGCATTGAAGT+AGG + Chr6:23227129-23227148 None:intergenic 45.0%
TTGGTACCTAGAAGAGTCGT+TGG + Chr6:23226728-23226747 None:intergenic 45.0%
TTGGTTTCTAAACTGGCGGT+CGG + Chr6:23226709-23226728 None:intergenic 45.0%
! AAAAGTGAACACGAGCACTG+TGG - Chr6:23226411-23226430 MsG0680031614.01.T01:intron 45.0%
! GATTTTGTCAAACTCAGCGC+TGG + Chr6:23226045-23226064 None:intergenic 45.0%
!! GTCGTTGGTTTCTAAACTGG+CGG + Chr6:23226713-23226732 None:intergenic 45.0%
AGAAATGTTGCTGTCGGTGC+AGG - Chr6:23226619-23226638 MsG0680031614.01.T01:intron 50.0%
AGAGCTACCCTGCATCTTCA+TGG - Chr6:23227019-23227038 MsG0680031614.01.T01:intron 50.0%
AGGAGATAGCCCCTGGTTTA+TGG - Chr6:23226014-23226033 MsG0680031614.01.T01:CDS 50.0%
CACAGATATTGGACCAACGG+TGG - Chr6:23226589-23226608 MsG0680031614.01.T01:intron 50.0%
GAAATGTTGCTGTCGGTGCA+GGG - Chr6:23226620-23226639 MsG0680031614.01.T01:intron 50.0%
GCCTCTCTCGATGAATCCAA+AGG - Chr6:23226829-23226848 MsG0680031614.01.T01:intron 50.0%
GTAACATAGGAGATAGCCCC+TGG - Chr6:23226007-23226026 MsG0680031614.01.T01:CDS 50.0%
GTGACAAACGGTCATACTCG+AGG - Chr6:23226952-23226971 MsG0680031614.01.T01:intron 50.0%
! GGTAATGGCACACCAATCCT+TGG - Chr6:23227324-23227343 MsG0680031614.01.T01:CDS 50.0%
ACTGGCGGTCGGAATTCCAT+CGG + Chr6:23226698-23226717 None:intergenic 55.0%
CAACTCCGACAGAAATGCCG+AGG + Chr6:23226568-23226587 None:intergenic 55.0%
! GATGCAGGGTAGCTCTGCAT+AGG + Chr6:23227015-23227034 None:intergenic 55.0%
TTGCTGTCGGTGCAGGGAGA+CGG - Chr6:23226626-23226645 MsG0680031614.01.T01:intron 60.0%
GTGTCACGCGCCGAGGGTTG+CGG - Chr6:23227143-23227162 MsG0680031614.01.T01:intron 70.0%
!! GCAGTGGTGTCACGCGCCGA+GGG - Chr6:23227137-23227156 MsG0680031614.01.T01:intron 70.0%
!! TGCAGTGGTGTCACGCGCCG+AGG - Chr6:23227136-23227155 MsG0680031614.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 23225264 23227359 23225264 ID=MsG0680031614.01;Name=MsG0680031614.01
Chr6 mRNA 23225264 23227359 23225264 ID=MsG0680031614.01.T01;Parent=MsG0680031614.01;Name=MsG0680031614.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|383
Chr6 exon 23227252 23227359 23227252 ID=MsG0680031614.01.T01:exon:14487;Parent=MsG0680031614.01.T01
Chr6 exon 23225264 23226307 23225264 ID=MsG0680031614.01.T01:exon:14486;Parent=MsG0680031614.01.T01
Chr6 CDS 23227252 23227359 23227252 ID=MsG0680031614.01.T01:cds;Parent=MsG0680031614.01.T01
Chr6 CDS 23225264 23226307 23225264 ID=MsG0680031614.01.T01:cds;Parent=MsG0680031614.01.T01
Gene Sequence

>MsG0680031614.01.T01

ATGAATGGTGAAGGTGACATGTTTGTTAAAGATTCTTCTAGTTTCATGCTCTTTGGTAAGAAGATTCCGGTGAGTTCTTCTCAGATTCAGGCCAATTCTACAACCATGAATTCTTGCAGTAACTCAAAGCATAAAGGAAATGATATGCCTCATACTCAAAACATTGAGAAATCAAAGAATTCTTCTAATGAAGAAAATGAATCAAAAGTGAACACGAGCACTGTGGAAGATAATATGGAAACTAGCAGCACAGATCAAGACAAAGTCCTAAAGAAACCAGACAAGATTGTTCAATGTCCGAGATGCAAAAGTTTCGACACAAAATTTTGTTATTTCAACAACTACAACGTCAATCAACCTCGGCATTTCTGTCGGAGTTGTCACAGATATTGGACCAACGGTGGAACAATGAGAAATGTTGCTGTCGGTGCAGGGAGACGGAAGAACAAACACATAGCTTCTCAATATCGCCACATGATTGTAGTGTCCGATGGAATTCCGACCGCCAGTTTAGAAACCAACGACTCTTCTAGGTACCAACATGGCTCAAATGTTGAATCTGCTGCGGTTTTTAGGTGTTCAAATGATAATGGTATTGTCCTAAAATTTGGTCGGGAAAATGCCTCTCTCGATGAATCCAAAGGTTCAATGCTTAATCTTATGAACCACAAGAAAAATGTTGATGCAAGTGGTAACAATTGTAGAGAAAATGGAGAGGAAGAATCATCGGTATGTGTATCGTCTGTGACAAACGGTCATACTCGAGGAAACGAGTTATTCGAATCCGAACAGAATAGGTCTAAACCTATGCAGAGCTACCCTGCATCTTCATGGATAATTCCAATGAATCAAAGTTGGAACAATGTTACTTCTATGGTTCAATCATCAATGCAGATGTGTAACCCTTATGGCATTGATCCTACTTCAATGCAGTGGTGTCACGCGCCGAGGGTTGCGGTTACAAACATTTTCACACCAAACATTGGCTTGCAATTTGTTCCTGCATCAAACAGGAATGGAACACCTTTGTATGGTAGTGGAAATGGAAATGTGTCCATAGGTTCAAATGGTTGCATTTCACCTTCTTCATCTACTACTAGTAATAGTTGTATCTCAGGTAATGGCACACCAATCCTTGGAAAACACACATGA
Protein sequence

>MsG0680031614.01.T01

MNGEGDMFVKDSSSFMLFGKKIPVSSSQIQANSTTMNSCSNSKHKGNDMPHTQNIEKSKNSSNEENESKVNTSTVEDNMETSSTDQDKVLKKPDKIVQCPRCKSFDTKFCYFNNYNVNQPRHFCRSCHRYWTNGGTMRNVAVGAGRRKNKHIASQYRHMIVVSDGIPTASLETNDSSRYQHGSNVESAAVFRCSNDNGIVLKFGRENASLDESKGSMLNLMNHKKNVDASGNNCRENGEEESSVCVSSVTNGHTRGNELFESEQNRSKPMQSYPASSWIIPMNQSWNNVTSMVQSSMQMCNPYGIDPTSMQWCHAPRVAVTNIFTPNIGLQFVPASNRNGTPLYGSGNGNVSIGSNGCISPSSSTTSNSCISGNGTPILGKHT*