AlfalfaGEDB | MsG0680032152.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032152.01

KEGG: -
GO: GO:0003700 GO:0006355
PFAM: PF00170

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032152.01.T01	XP_024634655.1	75.845	207	35	1	1	207	1	192	8.15E-101	301

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032152.01.T01	F4IN23	41.071	112	66	0	84	195	169	280	1.52E-18	86.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032152.01.T01	A0A2K3MUT1	54.922	193	84	3	7	198	1	191	7.24e-56	186

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TFs/TRs:

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Gene ID	Type	Classification
MsG0680032152.01.T01	TF	bZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032152.01.T01	MTR_3g465280	64.000	75	23	3	15	88	9	80	1.53e-23	96.7
MsG0680032152.01.T01	MTR_3g465280	42.342	111	54	5	2	110	91	193	9.12e-15	72.4
MsG0680032152.01.T01	MTR_4g079500	44.211	95	53	0	101	195	194	288	1.91e-18	83.2
MsG0680032152.01.T01	MTR_2g090900	43.158	95	54	0	101	195	191	285	1.77e-17	80.5
MsG0680032152.01.T01	MTR_2g090900	43.158	95	54	0	101	195	191	285	2.72e-17	79.7
MsG0680032152.01.T01	MTR_6g034945	35.088	114	74	0	100	213	150	263	1.52e-11	63.5
MsG0680032152.01.T01	MTR_5g075390	33.071	127	77	1	75	193	125	251	2.51e-11	63.2
MsG0680032152.01.T01	MTR_3g467120	38.298	94	58	0	100	193	176	269	3.05e-11	62.8
MsG0680032152.01.T01	MTR_4g072090	35.922	103	66	0	101	203	360	462	7.22e-11	62.0
MsG0680032152.01.T01	MTR_2g099050	36.458	96	61	0	100	195	220	315	8.00e-11	61.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032152.01.T01	AT2G42380	41.071	112	66	0	84	195	169	280	1.55e-19	86.3
MsG0680032152.01.T01	AT3G58120	40.708	113	66	1	83	195	185	296	9.01e-19	84.0
MsG0680032152.01.T01	AT2G42380	42.708	96	55	0	84	179	169	264	4.87e-17	78.6
MsG0680032152.01.T01	AT3G58120	42.268	97	55	1	83	179	185	280	2.87e-16	76.6
MsG0680032152.01.T01	AT2G42380	35.714	112	61	1	84	195	169	269	1.31e-13	69.3
MsG0680032152.01.T01	AT5G07160	40.860	93	55	0	101	193	48	140	4.31e-12	62.4
MsG0680032152.01.T01	AT2G40620	38.298	94	58	0	100	193	147	240	3.83e-11	62.4
MsG0680032152.01.T01	AT1G43700	39.362	94	57	0	100	193	193	286	5.40e-11	62.0
MsG0680032152.01.T01	AT2G31370	35.849	106	68	0	100	205	200	305	7.71e-11	61.6
MsG0680032152.01.T01	AT1G06070	37.500	96	60	0	100	195	209	304	9.56e-11	61.6

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Find 49 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGAGGCGAGTAGCAG+TGG	0.525757	6:+35063036	MsG0680032152.01.T01:CDS
AAACATGTTGCACACAGAAG+AGG	0.606246	6:+35062912	MsG0680032152.01.T01:CDS
AAAGAAAGGATACTTCAAGA+AGG	0.624481	6:+35064438	MsG0680032152.01.T01:CDS
AAATTGGAGAGGAATCGTCA+AGG	0.703846	6:+35065503	MsG0680032152.01.T01:CDS
AACATGTTGCACACAGAAGA+GGG	0.550817	6:+35062913	MsG0680032152.01.T01:CDS
AGACTTCAACTTGTGGGCGA+AGG	0.559195	6:-35064347	None:intergenic
AGCTGAGGTGAATAGGCTGA+GGG	0.614102	6:+35064568	MsG0680032152.01.T01:CDS
AGGTCAATGCTCTTCCAAGA+GGG	0.634364	6:-35063009	None:intergenic
AGTGTACCAAAATCAAATCT+TGG	0.459311	6:+35063099	MsG0680032152.01.T01:CDS
ATAAGAGAGCTGAGGTGAAT+AGG	0.348904	6:+35064561	MsG0680032152.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAAGGATACTTCAAGA+AGG	+	Chr6:35064438-35064457	MsG0680032152.01.T01:CDS	30.0%
AAATTCAGATCCAAAAAGCA+GGG	-	Chr6:35065053-35065072	None:intergenic	30.0%
AATTCTGTGATTTATTCGAG+CGG	+	Chr6:35065217-35065236	MsG0680032152.01.T01:intron	30.0%
AGATCAAACGGTTCAAATTA+AGG	-	Chr6:35065164-35065183	None:intergenic	30.0%
AGTGTACCAAAATCAAATCT+TGG	+	Chr6:35063099-35063118	MsG0680032152.01.T01:CDS	30.0%
AGTTTGATGGGAATAGTATT+TGG	+	Chr6:35063318-35063337	MsG0680032152.01.T01:intron	30.0%
CAAATCAACTATATTAGCCA+GGG	-	Chr6:35063558-35063577	None:intergenic	30.0%
GAATTTCCTATTGTTAGAAC+AGG	+	Chr6:35065067-35065086	MsG0680032152.01.T01:intron	30.0%
GATCAAACGGTTCAAATTAA+GGG	-	Chr6:35065163-35065182	None:intergenic	30.0%
TATAATTATATGTGTGCTCG+CGG	+	Chr6:35064912-35064931	MsG0680032152.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	35062901	35065585	35062901	ID=MsG0680032152.01;Name=MsG0680032152.01
Chr6	mRNA	35062901	35065585	35062901	ID=MsG0680032152.01.T01;Parent=MsG0680032152.01;Name=MsG0680032152.01.T01;_AED=0.12;_eAED=0.19;_QI=0\|0\|0\|0.8\|0.75\|0.8\|5\|0\|241
Chr6	exon	35062901	35063220	35062901	ID=MsG0680032152.01.T01:exon:19050;Parent=MsG0680032152.01.T01
Chr6	exon	35063706	35063796	35063706	ID=MsG0680032152.01.T01:exon:19051;Parent=MsG0680032152.01.T01
Chr6	exon	35064333	35064459	35064333	ID=MsG0680032152.01.T01:exon:19052;Parent=MsG0680032152.01.T01
Chr6	exon	35064549	35064650	35064549	ID=MsG0680032152.01.T01:exon:19053;Parent=MsG0680032152.01.T01
Chr6	exon	35065500	35065585	35065500	ID=MsG0680032152.01.T01:exon:19054;Parent=MsG0680032152.01.T01
Chr6	CDS	35062901	35063220	35062901	ID=MsG0680032152.01.T01:cds;Parent=MsG0680032152.01.T01
Chr6	CDS	35063706	35063796	35063706	ID=MsG0680032152.01.T01:cds;Parent=MsG0680032152.01.T01
Chr6	CDS	35064333	35064459	35064333	ID=MsG0680032152.01.T01:cds;Parent=MsG0680032152.01.T01
Chr6	CDS	35064549	35064650	35064549	ID=MsG0680032152.01.T01:cds;Parent=MsG0680032152.01.T01
Chr6	CDS	35065500	35065585	35065500	ID=MsG0680032152.01.T01:cds;Parent=MsG0680032152.01.T01

Gene Sequence

>MsG0680032152.01.T01

ATGACTGGTACAAACATGTTGCACACAGAAGAGGGACCTGGGCCTAAGAAATCAAGCATGGTTAGACCTTCCTCATATGGATCGTCATCAACATTTCGATCATGGCAACCCTCTTGGAAGAGCATTGACCTCGGTAAACAAGAGGCGAGTAGCAGTGGAGTCAAAGCAACTCCACAAGACAAAAAAACTAATAACATTAGTGTACCAAAATCAAATCTTGGTCATGCTGATAAGAACCACATCAGTGGCGGTAGTGCATCTGCATGTGTTGGTGTTGGTGATCAAGCAACACAAAAACTGGATCCAAAGAGGCTCAAACGGATCCTAGCAAATCGAATTTCTGCACAAAAATCGAGATTGAAAAAGCTCGATTATATTGCTGATTTGGAGCGGATGGAAAAATATTTGCAGGCTGAGTTATCTTTCCTTCGCCCACAAGTTGAAGTCTACAAAAATCAGCAGTGGCTGTTGACCATGGAACACCACCAATTAAGACTTGAAATAGCTGTACGAGAAAAAGAAAGGATACTTCAAGAAGTGGAGATTGAGAATAAGAGAGCTGAGGTGAATAGGCTGAGGGAGCTTCAGAAGAAACTTGTTGCTGAAGCACAAAACAAGAGGTATATTGAAATGCTTACTACTGAAATTGGAGAGGAATCGTCAAGGATGATGCAAACTGTTACTACTATGCCAATCAGACCAAATGAGCATAATATGGAGAAATAA

Protein sequence

>MsG0680032152.01.T01

MTGTNMLHTEEGPGPKKSSMVRPSSYGSSSTFRSWQPSWKSIDLGKQEASSSGVKATPQDKKTNNISVPKSNLGHADKNHISGGSASACVGVGDQATQKLDPKRLKRILANRISAQKSRLKKLDYIADLERMEKYLQAELSFLRPQVEVYKNQQWLLTMEHHQLRLEIAVREKERILQEVEIENKRAEVNRLRELQKKLVAEAQNKRYIEMLTTEIGEESSRMMQTVTTMPIRPNEHNMEK*