AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033323.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033323.01.T01 MTR_1g048120 67.429 175 33 1 38 188 172 346 1.60e-75 234
MsG0680033323.01.T01 MTR_2g461810 62.712 177 56 1 21 187 373 549 4.34e-67 221
MsG0680033323.01.T01 MTR_4g044357 59.459 111 44 1 78 188 2 111 8.65e-39 135
MsG0680033323.01.T01 MTR_0319s0030 42.308 182 75 4 37 189 196 376 2.81e-37 136
MsG0680033323.01.T01 MTR_4g046867 41.718 163 67 3 25 187 13 147 2.58e-34 122
MsG0680033323.01.T01 MTR_0046s0090 44.248 113 51 3 78 187 6 109 1.26e-22 95.9
MsG0680033323.01.T01 MTR_0013s0330 39.362 94 53 2 37 126 317 410 6.62e-13 68.6
MsG0680033323.01.T01 MTR_0013s0190 39.362 94 53 2 37 126 317 410 6.81e-13 68.6
MsG0680033323.01.T01 MTR_0401s0010 40.517 116 53 5 79 189 539 643 8.10e-13 68.6
MsG0680033323.01.T01 MTR_1g016990 47.222 72 32 1 62 127 17 88 1.01e-12 65.9
MsG0680033323.01.T01 MTR_4g051542 75.676 37 9 0 151 187 61 97 1.80e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033323.01.T01 AT2G32250 58.442 77 31 1 198 273 41 117 1.67e-20 91.7
MsG0680033323.01.T01 AT2G32250 58.442 77 31 1 198 273 41 117 1.67e-20 91.7
MsG0680033323.01.T01 AT2G32250 58.442 77 31 1 198 273 41 117 1.67e-20 91.7
MsG0680033323.01.T01 AT2G32250 58.442 77 31 1 198 273 41 117 1.69e-20 91.7
MsG0680033323.01.T01 AT2G32250 58.442 77 31 1 198 273 41 117 1.69e-20 91.7
MsG0680033323.01.T01 AT2G32250 58.442 77 31 1 198 273 41 117 1.72e-20 91.3
MsG0680033323.01.T01 AT2G32250 57.895 76 31 1 199 273 1 76 1.32e-19 89.0
MsG0680033323.01.T01 AT2G32250 57.895 76 31 1 199 273 1 76 1.38e-19 89.0
MsG0680033323.01.T01 AT1G76320 52.632 76 35 1 199 273 1 76 2.35e-18 85.1
MsG0680033323.01.T01 AT1G76320 52.632 76 35 1 199 273 1 76 2.42e-18 85.1
MsG0680033323.01.T01 AT1G76320 52.632 76 35 1 199 273 1 76 2.42e-18 85.1
MsG0680033323.01.T01 AT1G76320 52.632 76 35 1 199 273 1 76 2.93e-18 84.7
MsG0680033323.01.T01 AT3G22170 34.848 132 67 4 159 273 36 165 1.13e-17 83.2
MsG0680033323.01.T01 AT3G22170 34.848 132 67 4 159 273 36 165 1.13e-17 83.2
MsG0680033323.01.T01 AT4G19990 36.634 101 47 1 190 273 16 116 6.91e-13 68.9
MsG0680033323.01.T01 AT4G19990 36.634 101 47 1 190 273 16 116 6.91e-13 68.9
MsG0680033323.01.T01 AT4G19990 36.634 101 47 1 190 273 16 116 7.72e-13 68.9
MsG0680033323.01.T01 AT4G19990 36.634 101 47 1 190 273 30 130 8.06e-13 68.6
MsG0680033323.01.T01 AT4G19990 36.634 101 47 1 190 273 16 116 1.01e-12 68.6
MsG0680033323.01.T01 AT4G19990 36.634 101 47 1 190 273 16 116 1.01e-12 68.6
MsG0680033323.01.T01 AT4G15090 39.286 84 47 1 194 273 50 133 2.95e-12 67.0
MsG0680033323.01.T01 AT4G15090 39.286 84 47 1 194 273 50 133 2.95e-12 67.0
MsG0680033323.01.T01 AT4G15090 39.286 84 47 1 194 273 50 133 2.95e-12 67.0
MsG0680033323.01.T01 AT4G15090 39.286 84 47 1 194 273 50 133 2.95e-12 67.0
MsG0680033323.01.T01 AT4G15090 39.286 84 47 1 194 273 50 133 2.95e-12 67.0

Find 62 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAATGTCATATGTAATT+TGG 0.153214 6:-62256005 MsG0680033323.01.T01:CDS
ATCGTATTCAGCTGGTAAAA+TGG 0.345375 6:+62255979 None:intergenic
TTCCAATGTAAGGTTCTTCA+TGG 0.353929 6:+62254414 None:intergenic
CAATCTCATCCACTTTGGTT+TGG 0.364303 6:+62255792 None:intergenic
ACAAAATCCACGTCCTTGTT+TGG 0.367076 6:-62254225 MsG0680033323.01.T01:CDS
TTACATATGACATTGAAGTT+TGG 0.386313 6:+62256010 None:intergenic
TGGATGAACAAAGGTTTGAT+AGG 0.390543 6:+62255812 None:intergenic
CCTAACTCGTTATCCTCATT+AGG 0.401505 6:+62256055 None:intergenic
TCAAATTCCATTCCAATGTA+AGG 0.402468 6:+62254404 None:intergenic
ACCATTCTCTGATCACCCCT+TGG 0.406231 6:+62256136 None:intergenic
CCTAATGAGGATAACGAGTT+AGG 0.412030 6:-62256055 MsG0680033323.01.T01:CDS
AAATGCCTTTCTTTGACCTC+TGG 0.434830 6:+62256188 None:intergenic
TATGCTAAATGTGTCGGATT+TGG 0.446775 6:-62254347 MsG0680033323.01.T01:CDS
TGCCTCAATCCTTACTCAAA+AGG 0.450878 6:-62255724 MsG0680033323.01.T01:CDS
AGAGAATGGTAGAATCCAAT+AGG 0.465476 6:-62256123 MsG0680033323.01.T01:CDS
TTAGGATCCTCTTCGACTAC+AGG 0.472107 6:+62256073 None:intergenic
CAAACCAAAGTGGATGAGAT+TGG 0.475050 6:-62255791 MsG0680033323.01.T01:CDS
CCATTCTCTGATCACCCCTT+GGG 0.481425 6:+62256137 None:intergenic
AATACCAATCTCATCCACTT+TGG 0.489873 6:+62255787 None:intergenic
TCCACGTCCTTGTTTGGAGG+TGG 0.493252 6:-62254219 MsG0680033323.01.T01:CDS
CTTGTTTGGAGGTGGAGTGT+GGG 0.499768 6:-62254211 MsG0680033323.01.T01:CDS
CCTTGTTTGGAGGTGGAGTG+TGG 0.503398 6:-62254212 MsG0680033323.01.T01:CDS
TGCTTGTCGCAACAACTACA+AGG 0.505196 6:-62256249 MsG0680033323.01.T01:CDS
CAATCCTTACTCAAAAGGAT+TGG 0.513342 6:-62255719 MsG0680033323.01.T01:intron
TTGTTTGGAGGTGGAGTGTG+GGG 0.538731 6:-62254210 MsG0680033323.01.T01:CDS
AAGATGAAATTGTTGACCCA+AGG 0.542186 6:-62256153 MsG0680033323.01.T01:CDS
GTACATATCAAAAGAAATTG+AGG 0.547372 6:-62254182 None:intergenic
AGCAAACTTCTTCGAATCAA+AGG 0.547639 6:+62256268 None:intergenic
TGAAGAACCTTACATTGGAA+TGG 0.550850 6:-62254411 MsG0680033323.01.T01:CDS
CCACACTCCACCTCCAAACA+AGG 0.558039 6:+62254212 None:intergenic
CCCAAGGGGTGATCAGAGAA+TGG 0.558920 6:-62256137 MsG0680033323.01.T01:CDS
GCTCGATATGCTAAATGTGT+CGG 0.559196 6:-62254353 MsG0680033323.01.T01:CDS
GATATCATATCGTATTCAGC+TGG 0.560548 6:+62255971 None:intergenic
TTCTCTTCCTGTAGTCGAAG+AGG 0.561117 6:-62256080 MsG0680033323.01.T01:CDS
TTTGTTCATCCAAACCAAAG+TGG 0.567906 6:-62255801 MsG0680033323.01.T01:CDS
AACCATGAAGAACCTTACAT+TGG 0.573124 6:-62254416 MsG0680033323.01.T01:CDS
AAATCCACGTCCTTGTTTGG+AGG 0.575635 6:-62254222 MsG0680033323.01.T01:CDS
TTCCTCAGAATCATCAACTT+CGG 0.576617 6:+62255949 None:intergenic
GATTGGTATTAACAAAGTGT+TGG 0.591440 6:-62255774 MsG0680033323.01.T01:CDS
AGAGTTCAATGTCAAAGACA+TGG 0.594302 6:-62255927 MsG0680033323.01.T01:CDS
GAGTTCAATGTCAAAGACAT+GGG 0.595151 6:-62255926 MsG0680033323.01.T01:CDS
ACTTTGGTTTGGATGAACAA+AGG 0.596464 6:+62255803 None:intergenic
ATCCGAAGTTGATGATTCTG+AGG 0.597392 6:-62255951 MsG0680033323.01.T01:CDS
TTTGTTGTTATGCACAACAC+TGG 0.605458 6:+62256301 None:intergenic
AACAAAGTGTTGGTCGACGG+TGG 0.609089 6:-62255764 MsG0680033323.01.T01:CDS
TCCACCTCCAAACAAGGACG+TGG 0.618921 6:+62254218 None:intergenic
ACGTACCAGAGGTCAAAGAA+AGG 0.620238 6:-62256193 MsG0680033323.01.T01:CDS
TGAGGATAACGAGTTAGGAT+CGG 0.625282 6:-62256050 MsG0680033323.01.T01:CDS
ATTAACAAAGTGTTGGTCGA+CGG 0.629723 6:-62255767 MsG0680033323.01.T01:CDS
AGTCGAAGAGGATCCTAATG+AGG 0.631571 6:-62256068 MsG0680033323.01.T01:CDS
CTCAGTGGAGGACGTACCAG+AGG 0.637453 6:-62256204 MsG0680033323.01.T01:CDS
ATAGGAGACCAGCAAAAGAG+AGG 0.638075 6:-62256105 MsG0680033323.01.T01:CDS
AAATATGCTTGCACGAGACA+TGG 0.643012 6:-62254269 MsG0680033323.01.T01:CDS
AGTTCAATGTCAAAGACATG+GGG 0.651086 6:-62255925 MsG0680033323.01.T01:CDS
AGATGAAATTGTTGACCCAA+GGG 0.668558 6:-62256152 MsG0680033323.01.T01:CDS
GCTTGTCGCAACAACTACAA+GGG 0.678998 6:-62256248 MsG0680033323.01.T01:CDS
ACATGGGAAGAACAATGTGG+TGG 0.685952 6:-62256333 MsG0680033323.01.T01:CDS
ATCACATGACAAGAACTCAG+TGG 0.708392 6:-62256219 MsG0680033323.01.T01:CDS
ACATGACAAGAACTCAGTGG+AGG 0.709540 6:-62256216 MsG0680033323.01.T01:CDS
GATGAAATTGTTGACCCAAG+GGG 0.723057 6:-62256151 MsG0680033323.01.T01:CDS
GTTCAATGTCAAAGACATGG+GGG 0.733079 6:-62255924 MsG0680033323.01.T01:CDS
GCAACATGGGAAGAACAATG+TGG 0.820067 6:-62256336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTAAAAAAAAAAAAA+AGG - Chr6:62255703-62255722 MsG0680033323.01.T01:intron 0.0%
!!! TTTTTTTTTTTTTTAATAAA+TGG + Chr6:62255704-62255723 None:intergenic 0.0%
!! AATACATATATAAAATATTG+GGG + Chr6:62254841-62254860 None:intergenic 10.0%
!! AAAAAAAAAAAAGGAATCAT+TGG - Chr6:62255712-62255731 MsG0680033323.01.T01:intron 15.0%
!!! AAAACATATTAATCTAAAGT+CGG - Chr6:62254948-62254967 MsG0680033323.01.T01:intron 15.0%
!!! AATATGTTTTGATTAAAAGA+AGG + Chr6:62254938-62254957 None:intergenic 15.0%
!!! ACTTCTTTTATACATATATT+AGG - Chr6:62255987-62256006 MsG0680033323.01.T01:CDS 15.0%
!!! CATCGTATTTAATATTTTTT+TGG - Chr6:62255941-62255960 MsG0680033323.01.T01:CDS 15.0%
!! AATTAAATTGAGTTTAGTCT+TGG + Chr6:62255234-62255253 None:intergenic 20.0%
!! ATACATATATTAGGAACTAA+TGG - Chr6:62255996-62256015 MsG0680033323.01.T01:CDS 20.0%
!! ATTTCTTAATTATCAGAAAG+TGG - Chr6:62255746-62255765 MsG0680033323.01.T01:CDS 20.0%
!!! ACAAAAGACAATTTTTTTAC+AGG + Chr6:62255852-62255871 None:intergenic 20.0%
!!! AGGATTACTATTTTTGATTT+AGG + Chr6:62255301-62255320 None:intergenic 20.0%
!!! TCAATTTCTTTTGATATGTA+CGG + Chr6:62256334-62256353 None:intergenic 20.0%
!!! TTATTTGACTTAAATTTACG+TGG + Chr6:62255344-62255363 None:intergenic 20.0%
! AAAAGTCATATACTACACAT+AGG + Chr6:62255881-62255900 None:intergenic 25.0%
! AAATTAACAGCCAATAGAAA+TGG + Chr6:62255414-62255433 None:intergenic 25.0%
! AATCACTTTGTAAAGGAAAA+TGG + Chr6:62255678-62255697 None:intergenic 25.0%
! CTTCAATGTCATATGTAATT+TGG - Chr6:62254510-62254529 MsG0680033323.01.T01:intron 25.0%
! GGTATGTAATGATCAATTAA+CGG - Chr6:62255962-62255981 MsG0680033323.01.T01:CDS 25.0%
! TCATTCACAAAATAACACAT+GGG + Chr6:62254625-62254644 None:intergenic 25.0%
! TTACATATGACATTGAAGTT+TGG + Chr6:62254508-62254527 None:intergenic 25.0%
!!! AAATCAAGAAAGGCATTAAA+TGG + Chr6:62255518-62255537 None:intergenic 25.0%
!!! ATGTGTTATTTTGTGAATGA+CGG - Chr6:62254625-62254644 MsG0680033323.01.T01:intron 25.0%
AAACTCATGACTAAGAAGTA+AGG + Chr6:62255121-62255140 None:intergenic 30.0%
AACTCATGACTAAGAAGTAA+GGG + Chr6:62255120-62255139 None:intergenic 30.0%
ACATATCATTCACTATTGAC+AGG + Chr6:62256060-62256079 None:intergenic 30.0%
ACCTTCATCTAAATCAAGAA+AGG + Chr6:62255528-62255547 None:intergenic 30.0%
ACGTGGACATAGATTTAAAA+TGG + Chr6:62255327-62255346 None:intergenic 30.0%
ACTTTGTAAAGGAAAATGGT+GGG + Chr6:62255674-62255693 None:intergenic 30.0%
ATGGACAAATCACTTTGTAA+AGG + Chr6:62255685-62255704 None:intergenic 30.0%
CTCATGAAAACCATTTCTAT+TGG - Chr6:62255401-62255420 MsG0680033323.01.T01:intron 30.0%
CTTAACTACCTTAGAATTAC+TGG - Chr6:62255056-62255075 MsG0680033323.01.T01:intron 30.0%
CTTGGAAGATAATACATGAA+CGG + Chr6:62255216-62255235 None:intergenic 30.0%
GATTGGTATTAACAAAGTGT+TGG - Chr6:62254741-62254760 MsG0680033323.01.T01:intron 30.0%
GTCATTCACAAAATAACACA+TGG + Chr6:62254626-62254645 None:intergenic 30.0%
GTTATTGGAGAAAAATCAAG+AGG + Chr6:62255192-62255211 None:intergenic 30.0%
TAATACATGAACGGAGTTAT+TGG + Chr6:62255207-62255226 None:intergenic 30.0%
TCAAATTCCATTCCAATGTA+AGG + Chr6:62256114-62256133 None:intergenic 30.0%
! ACATAGATTTAAAATGGGGA+AGG + Chr6:62255321-62255340 None:intergenic 30.0%
! CGTGGACATAGATTTAAAAT+GGG + Chr6:62255326-62255345 None:intergenic 30.0%
! GATCAATTTGTTTCCTCTTA+TGG - Chr6:62255372-62255391 MsG0680033323.01.T01:intron 30.0%
! GTGGACATAGATTTAAAATG+GGG + Chr6:62255325-62255344 None:intergenic 30.0%
!! ATGATATGTTGCTGATTTAG+AGG - Chr6:62256071-62256090 MsG0680033323.01.T01:CDS 30.0%
AACCATGAAGAACCTTACAT+TGG - Chr6:62256099-62256118 MsG0680033323.01.T01:CDS 35.0%
AACTCCTACCAGTAATTCTA+AGG + Chr6:62255067-62255086 None:intergenic 35.0%
AAGACTACACACTTGTAATG+AGG + Chr6:62255462-62255481 None:intergenic 35.0%
AAGATGAAATTGTTGACCCA+AGG - Chr6:62254362-62254381 MsG0680033323.01.T01:CDS 35.0%
AATACCAATCTCATCCACTT+TGG + Chr6:62254731-62254750 None:intergenic 35.0%
ACTACCTTAGAATTACTGGT+AGG - Chr6:62255060-62255079 MsG0680033323.01.T01:intron 35.0%
AGACTACACACTTGTAATGA+GGG + Chr6:62255461-62255480 None:intergenic 35.0%
AGAGAATGGTAGAATCCAAT+AGG - Chr6:62254392-62254411 MsG0680033323.01.T01:CDS 35.0%
AGAGTTCAATGTCAAAGACA+TGG - Chr6:62254588-62254607 MsG0680033323.01.T01:intron 35.0%
AGATGAAATTGTTGACCCAA+GGG - Chr6:62254363-62254382 MsG0680033323.01.T01:CDS 35.0%
AGCAAACTTCTTCGAATCAA+AGG + Chr6:62254250-62254269 None:intergenic 35.0%
AGTCATATACTACACATAGG+AGG + Chr6:62255878-62255897 None:intergenic 35.0%
AGTTCAATGTCAAAGACATG+GGG - Chr6:62254590-62254609 MsG0680033323.01.T01:intron 35.0%
ATCGTATTCAGCTGGTAAAA+TGG + Chr6:62254539-62254558 None:intergenic 35.0%
CAATCCTTACTCAAAAGGAT+TGG - Chr6:62254796-62254815 MsG0680033323.01.T01:intron 35.0%
CACTTTGTAAAGGAAAATGG+TGG + Chr6:62255675-62255694 None:intergenic 35.0%
GAGTTCAATGTCAAAGACAT+GGG - Chr6:62254589-62254608 MsG0680033323.01.T01:intron 35.0%
GAGTTGTACATTAGTTCACA+TGG - Chr6:62255082-62255101 MsG0680033323.01.T01:intron 35.0%
GATATCATATCGTATTCAGC+TGG + Chr6:62254547-62254566 None:intergenic 35.0%
GCCTTTCTTGATTTAGATGA+AGG - Chr6:62255524-62255543 MsG0680033323.01.T01:intron 35.0%
GGCATTAAATGGCTTCTATT+TGG + Chr6:62255507-62255526 None:intergenic 35.0%
TACTTAGCGCCAATTTATCT+AGG + Chr6:62255622-62255641 None:intergenic 35.0%
TATGCTAAATGTGTCGGATT+TGG - Chr6:62256168-62256187 MsG0680033323.01.T01:CDS 35.0%
TGAAGAACCTTACATTGGAA+TGG - Chr6:62256104-62256123 MsG0680033323.01.T01:CDS 35.0%
TGATTTAGGCAAGTAACGTT+TGG + Chr6:62255287-62255306 None:intergenic 35.0%
TGGATGAACAAAGGTTTGAT+AGG + Chr6:62254706-62254725 None:intergenic 35.0%
TGGTAAAAAACGAGAATCGA+CGG - Chr6:62256266-62256285 MsG0680033323.01.T01:CDS 35.0%
TTCCAATGTAAGGTTCTTCA+TGG + Chr6:62256104-62256123 None:intergenic 35.0%
TTCCTCAGAATCATCAACTT+CGG + Chr6:62254569-62254588 None:intergenic 35.0%
TTTGTTCATCCAAACCAAAG+TGG - Chr6:62254714-62254733 MsG0680033323.01.T01:intron 35.0%
! ATATTGGGGTGTTACATTGT+CGG + Chr6:62254827-62254846 None:intergenic 35.0%
! CAAGAGGAGAGGTTTAAAAT+CGG + Chr6:62255176-62255195 None:intergenic 35.0%
! CACTCTTCATTTTCATCGAT+AGG + Chr6:62256024-62256043 None:intergenic 35.0%
! GAAATGGTTTTCATGAGACT+TGG + Chr6:62255398-62255417 None:intergenic 35.0%
! TAACGTTTGGTCTTCATACT+TGG + Chr6:62255274-62255293 None:intergenic 35.0%
!! ACTTTGGTTTGGATGAACAA+AGG + Chr6:62254715-62254734 None:intergenic 35.0%
!! AGAAAAGTGTTGTACGTCAT+TGG - Chr6:62255802-62255821 MsG0680033323.01.T01:CDS 35.0%
!! ATCCTTTTGAGTAAGGATTG+AGG + Chr6:62254796-62254815 None:intergenic 35.0%
!! ATTAACAAAGTGTTGGTCGA+CGG - Chr6:62254748-62254767 MsG0680033323.01.T01:intron 35.0%
!! CTTACCAATCCTTTTGAGTA+AGG + Chr6:62254803-62254822 None:intergenic 35.0%
!! TTTGTTGTTATGCACAACAC+TGG + Chr6:62254217-62254236 None:intergenic 35.0%
!!! CGTCATTGGTCATTTTTGTA+AGG - Chr6:62255816-62255835 MsG0680033323.01.T01:CDS 35.0%
AAATATGCTTGCACGAGACA+TGG - Chr6:62256246-62256265 MsG0680033323.01.T01:CDS 40.0%
AAATGCCTTTCTTTGACCTC+TGG + Chr6:62254330-62254349 None:intergenic 40.0%
ACAAAATCCACGTCCTTGTT+TGG - Chr6:62256290-62256309 MsG0680033323.01.T01:CDS 40.0%
ATCACATGACAAGAACTCAG+TGG - Chr6:62254296-62254315 MsG0680033323.01.T01:CDS 40.0%
ATCATCAGGCTGCTCAAATA+TGG + Chr6:62254671-62254690 None:intergenic 40.0%
ATGACGGCTCTTAAGACAAT+CGG - Chr6:62254641-62254660 MsG0680033323.01.T01:intron 40.0%
CAAACCAAAGTGGATGAGAT+TGG - Chr6:62254724-62254743 MsG0680033323.01.T01:intron 40.0%
CAATCTCATCCACTTTGGTT+TGG + Chr6:62254726-62254745 None:intergenic 40.0%
CCTAACTCGTTATCCTCATT+AGG + Chr6:62254463-62254482 None:intergenic 40.0%
CCTAATGAGGATAACGAGTT+AGG - Chr6:62254460-62254479 MsG0680033323.01.T01:intron 40.0%
GATGAAATTGTTGACCCAAG+GGG - Chr6:62254364-62254383 MsG0680033323.01.T01:CDS 40.0%
GCTCGATATGCTAAATGTGT+CGG - Chr6:62256162-62256181 MsG0680033323.01.T01:CDS 40.0%
GGATTCCTTATCGAATCATC+AGG + Chr6:62254685-62254704 None:intergenic 40.0%
GGTCTTCATACTTGGTCAAA+GGG + Chr6:62255266-62255285 None:intergenic 40.0%
GTTCAATGTCAAAGACATGG+GGG - Chr6:62254591-62254610 MsG0680033323.01.T01:intron 40.0%
TCATGAGTTTGATCGCAGTT+AGG - Chr6:62255131-62255150 MsG0680033323.01.T01:intron 40.0%
TGAGGATAACGAGTTAGGAT+CGG - Chr6:62254465-62254484 MsG0680033323.01.T01:intron 40.0%
TGCCTCAATCCTTACTCAAA+AGG - Chr6:62254791-62254810 MsG0680033323.01.T01:intron 40.0%
TGGAGAAAAATCAAGAGGAG+AGG + Chr6:62255187-62255206 None:intergenic 40.0%
TGGTCTTCATACTTGGTCAA+AGG + Chr6:62255267-62255286 None:intergenic 40.0%
TTCACTATTGACAGGCTCAT+TGG + Chr6:62256052-62256071 None:intergenic 40.0%
! AGAGAAAACCTCTCTTTTGC+TGG + Chr6:62254421-62254440 None:intergenic 40.0%
! GACTAAGAAGTAAGGGTACA+AGG + Chr6:62255113-62255132 None:intergenic 40.0%
! TATGGGAACCTCAAACGTTT+TGG - Chr6:62255594-62255613 MsG0680033323.01.T01:intron 40.0%
!! ATCCGAAGTTGATGATTCTG+AGG - Chr6:62254564-62254583 MsG0680033323.01.T01:intron 40.0%
!! GTTTTGGCGCCTAGATAAAT+TGG - Chr6:62255610-62255629 MsG0680033323.01.T01:intron 40.0%
ACATGACAAGAACTCAGTGG+AGG - Chr6:62254299-62254318 MsG0680033323.01.T01:CDS 45.0%
ACGTACCAGAGGTCAAAGAA+AGG - Chr6:62254322-62254341 MsG0680033323.01.T01:CDS 45.0%
AGCAGCCTGATGATTCGATA+AGG - Chr6:62254677-62254696 MsG0680033323.01.T01:intron 45.0%
AGTCGAAGAGGATCCTAATG+AGG - Chr6:62254447-62254466 MsG0680033323.01.T01:intron 45.0%
ATAGGAGACCAGCAAAAGAG+AGG - Chr6:62254410-62254429 MsG0680033323.01.T01:CDS 45.0%
ATGAGGGAGAGAGAGAGAAA+AGG + Chr6:62255445-62255464 None:intergenic 45.0%
GCTTGTCGCAACAACTACAA+GGG - Chr6:62254267-62254286 MsG0680033323.01.T01:CDS 45.0%
TCATGAGACTTGGCCATAAG+AGG + Chr6:62255388-62255407 None:intergenic 45.0%
TGCTTGTCGCAACAACTACA+AGG - Chr6:62254266-62254285 MsG0680033323.01.T01:CDS 45.0%
TTAGGATCCTCTTCGACTAC+AGG + Chr6:62254445-62254464 None:intergenic 45.0%
TTCTCTTCCTGTAGTCGAAG+AGG - Chr6:62254435-62254454 MsG0680033323.01.T01:intron 45.0%
! AAATCCACGTCCTTGTTTGG+AGG - Chr6:62256293-62256312 MsG0680033323.01.T01:CDS 45.0%
!! CTCTTTTGCTGGTCTCCTAT+TGG + Chr6:62254410-62254429 None:intergenic 45.0%
!! AAAATACATATATAAAATAT+TGG + Chr6:62254843-62254862 None:intergenic 5.0%
!! AAATACATATATAAAATATT+GGG + Chr6:62254842-62254861 None:intergenic 5.0%
!! AATTATTTAATTAAAATCTT+AGG + Chr6:62254993-62255012 None:intergenic 5.0%
!! ATAAATAAAAAACAATTAAA+TGG + Chr6:62255570-62255589 None:intergenic 5.0%
!!! GTTTTTTATTTATTTTTTAT+GGG - Chr6:62255577-62255596 MsG0680033323.01.T01:intron 5.0%
!!! TGTTTTTTATTTATTTTTTA+TGG - Chr6:62255576-62255595 MsG0680033323.01.T01:intron 5.0%
ACCATTCTCTGATCACCCCT+TGG + Chr6:62254382-62254401 None:intergenic 50.0%
AGGGAGAGAGAGAGAAAAGG+TGG + Chr6:62255442-62255461 None:intergenic 50.0%
CCATTCTCTGATCACCCCTT+GGG + Chr6:62254381-62254400 None:intergenic 50.0%
CTTGTTTGGAGGTGGAGTGT+GGG - Chr6:62256304-62256323 MsG0680033323.01.T01:CDS 50.0%
GGGAGAGAGAGAGAAAAGGT+GGG + Chr6:62255441-62255460 None:intergenic 50.0%
TCTAGGCGCCAAAACGTTTG+AGG + Chr6:62255605-62255624 None:intergenic 50.0%
TTGTTTGGAGGTGGAGTGTG+GGG - Chr6:62256305-62256324 MsG0680033323.01.T01:CDS 50.0%
!! AACAAAGTGTTGGTCGACGG+TGG - Chr6:62254751-62254770 MsG0680033323.01.T01:intron 50.0%
CCACACTCCACCTCCAAACA+AGG + Chr6:62256306-62256325 None:intergenic 55.0%
CCCAAGGGGTGATCAGAGAA+TGG - Chr6:62254378-62254397 MsG0680033323.01.T01:CDS 55.0%
TCCACCTCCAAACAAGGACG+TGG + Chr6:62256300-62256319 None:intergenic 55.0%
! CCTTGTTTGGAGGTGGAGTG+TGG - Chr6:62256303-62256322 MsG0680033323.01.T01:CDS 55.0%
! TCCACGTCCTTGTTTGGAGG+TGG - Chr6:62256296-62256315 MsG0680033323.01.T01:CDS 55.0%
CTCAGTGGAGGACGTACCAG+AGG - Chr6:62254311-62254330 MsG0680033323.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 62254184 62256353 62254184 ID=MsG0680033323.01;Name=MsG0680033323.01
Chr6 mRNA 62254184 62256353 62254184 ID=MsG0680033323.01.T01;Parent=MsG0680033323.01;Name=MsG0680033323.01.T01;_AED=0.62;_eAED=0.71;_QI=0|0|0|0.66|1|1|3|0|274
Chr6 exon 62255911 62256353 62255911 ID=MsG0680033323.01.T01:exon:7183;Parent=MsG0680033323.01.T01
Chr6 exon 62255720 62255838 62255720 ID=MsG0680033323.01.T01:exon:7184;Parent=MsG0680033323.01.T01
Chr6 exon 62254184 62254446 62254184 ID=MsG0680033323.01.T01:exon:7185;Parent=MsG0680033323.01.T01
Chr6 CDS 62255911 62256353 62255911 ID=MsG0680033323.01.T01:cds;Parent=MsG0680033323.01.T01
Chr6 CDS 62255720 62255838 62255720 ID=MsG0680033323.01.T01:cds;Parent=MsG0680033323.01.T01
Chr6 CDS 62254184 62254446 62254184 ID=MsG0680033323.01.T01:cds;Parent=MsG0680033323.01.T01
Gene Sequence

>MsG0680033323.01.T01

ATGGGAAGAACAATGTGGTGGAAAGAAACACCAGTGTTGTGCATAACAACAAAAATGCTACTGCCTTTGATTCGAAGAAGTTTGCTTGTCGCAACAACTACAAGGGAAAAAAATCACATGACAAGAACTCAGTGGAGGACGTACCAGAGGTCAAAGAAAGGCATTTCTACAAGCATTGAAGATGAAATTGTTGACCCAAGGGGTGATCAGAGAATGGTAGAATCCAATAGGAGACCAGCAAAAGAGAGGTTTTCTCTTCCTGTAGTCGAAGAGGATCCTAATGAGGATAACGAGTTAGGATCGGAATTTACAGATTCAGAACCAAACTTCAATGTCATATGTAATTTGGTTTCCATTTTACCAGCTGAATACGATATGATATCCGAAGTTGATGATTCTGAGGAAGAGTTCAATGTCAAAGACATGGGGGAATACAAGCCCATGAATCCTATCAAACCTTTGTTCATCCAAACCAAAGTGGATGAGATTGGTATTAACAAAGTGTTGGTCGACGGTGGCGCTGCTGTCAATCTAATGCCTCAATCCTTACTCAAAAGGATTGAGGTTAAGAACCATGAAGAACCTTACATTGGAATGGAATTTGAGTCACAAGAAGATGCTTATTCTTTCTATGCTCGATATGCTAAATGTGTCGGATTTGGTGTATGCACTAAAATTAGTCATCGTTCGAAAATTTCTAAACAATTTATTGATGTGAAATATGCTTGCACGAGACATGGTAAAAAACGAGAATCGACGGCACAAAATCCACGTCCTTGTTTGGAGGTGGAGTGTGGGGCTGCCGTACATATCAAAAGAAATTGA
Protein sequence

>MsG0680033323.01.T01

MGRTMWWKETPVLCITTKMLLPLIRRSLLVATTTREKNHMTRTQWRTYQRSKKGISTSIEDEIVDPRGDQRMVESNRRPAKERFSLPVVEEDPNEDNELGSEFTDSEPNFNVICNLVSILPAEYDMISEVDDSEEEFNVKDMGEYKPMNPIKPLFIQTKVDEIGINKVLVDGGAAVNLMPQSLLKRIEVKNHEEPYIGMEFESQEDAYSFYARYAKCVGFGVCTKISHRSKISKQFIDVKYACTRHGKKRESTAQNPRPCLEVECGAAVHIKRN*