AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033402.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033402.01.T01 MTR_6g045327 77.330 397 49 5 20 385 1 387 0.0 600
MsG0680033402.01.T01 MTR_4g021760 45.192 312 146 8 20 318 1 300 2.10e-69 221
MsG0680033402.01.T01 MTR_8g093040 47.638 254 115 4 20 273 1 236 3.30e-61 199
MsG0680033402.01.T01 MTR_4g021790 47.085 223 115 2 33 253 10 231 9.37e-60 195
MsG0680033402.01.T01 MTR_1g032570 41.455 275 122 5 16 258 1 268 4.12e-58 199
MsG0680033402.01.T01 MTR_4g021855 45.494 233 122 3 33 263 10 239 2.02e-57 189
MsG0680033402.01.T01 MTR_4g021845 44.595 222 116 3 20 239 1 217 1.55e-53 177
MsG0680033402.01.T01 MTR_4g121020 37.945 253 128 3 32 256 30 281 1.41e-48 174
MsG0680033402.01.T01 MTR_4g121020 37.945 253 128 3 32 256 30 281 1.42e-48 174
MsG0680033402.01.T01 MTR_2g034960 36.742 264 140 3 16 253 12 274 6.74e-48 172
MsG0680033402.01.T01 MTR_8g079940 38.017 242 123 3 33 247 25 266 5.88e-47 168
MsG0680033402.01.T01 MTR_3g086100 36.992 246 126 4 31 247 11 256 5.89e-46 166
MsG0680033402.01.T01 MTR_8g105600 40.928 237 117 3 33 247 9 244 4.53e-45 164
MsG0680033402.01.T01 MTR_3g106220 35.341 249 128 4 32 247 7 255 5.14e-39 147
MsG0680033402.01.T01 MTR_4g131600 32.510 243 140 2 28 246 15 257 5.33e-39 147
MsG0680033402.01.T01 MTR_1g013170 35.366 246 128 4 32 247 4 248 4.67e-38 143
MsG0680033402.01.T01 MTR_5g055260 41.667 216 100 5 33 248 10 199 6.39e-38 137
MsG0680033402.01.T01 MTR_4g131570 31.538 260 146 3 20 247 1 260 2.46e-37 142
MsG0680033402.01.T01 MTR_4g131580 32.510 243 138 2 31 247 15 257 2.88e-37 142
MsG0680033402.01.T01 MTR_3g102600 32.468 231 123 1 26 256 1 198 1.97e-36 132
MsG0680033402.01.T01 MTR_1g032570 40.102 197 86 4 94 258 3 199 3.29e-36 139
MsG0680033402.01.T01 MTR_0450s0040 33.333 222 140 1 37 250 8 229 4.81e-36 134
MsG0680033402.01.T01 MTR_7g117705 32.377 244 136 3 32 246 4 247 2.75e-34 132
MsG0680033402.01.T01 MTR_2g450070 30.303 264 146 3 23 248 8 271 3.04e-34 134
MsG0680033402.01.T01 MTR_2g450070 30.303 264 146 3 23 248 8 271 3.54e-34 134
MsG0680033402.01.T01 MTR_3g102600 32.243 214 112 1 26 239 1 181 3.20e-33 123
MsG0680033402.01.T01 MTR_1g013180 30.303 264 155 2 20 254 143 406 9.85e-33 130
MsG0680033402.01.T01 MTR_3g086100 36.216 185 89 4 92 247 1 185 1.86e-31 125
MsG0680033402.01.T01 MTR_7g026400 36.181 199 110 2 63 246 17 213 1.93e-31 125
MsG0680033402.01.T01 MTR_6g016850 42.073 164 90 4 28 189 4 164 6.06e-31 116
MsG0680033402.01.T01 MTR_3g102590 33.636 220 120 3 26 243 1 196 1.12e-29 115
MsG0680033402.01.T01 MTR_3g102590 33.028 218 120 3 26 241 1 194 1.77e-28 110
MsG0680033402.01.T01 MTR_3g102600 31.319 182 92 1 75 256 1 149 9.41e-28 108
MsG0680033402.01.T01 MTR_4g121020 35.465 172 84 1 112 256 6 177 1.13e-27 114
MsG0680033402.01.T01 MTR_4g023980 40.714 140 75 3 80 214 36 172 8.28e-27 105
MsG0680033402.01.T01 MTR_3g102600 30.909 165 81 1 75 239 1 132 6.58e-25 99.4
MsG0680033402.01.T01 MTR_4g064730 49.296 71 33 1 189 259 130 197 4.57e-14 72.8
MsG0680033402.01.T01 MTR_7g089010 54.237 59 27 0 189 247 90 148 8.22e-14 72.0
MsG0680033402.01.T01 MTR_5g014040 34.109 129 81 2 31 158 12 137 2.43e-13 71.6
MsG0680033402.01.T01 MTR_5g014040 45.455 55 30 0 189 243 310 364 7.28e-11 63.9
MsG0680033402.01.T01 MTR_5g014040 34.109 129 81 2 31 158 12 137 2.60e-13 71.6
MsG0680033402.01.T01 MTR_1g013160 25.503 149 82 2 95 214 1 149 4.82e-13 67.0
MsG0680033402.01.T01 MTR_5g041350 54.545 55 24 1 190 243 15 69 5.71e-12 67.0
MsG0680033402.01.T01 MTR_4g081710 50.909 55 26 1 190 243 17 71 4.39e-11 63.9
MsG0680033402.01.T01 MTR_4g098870 45.455 55 30 0 189 243 309 363 4.80e-11 64.7
MsG0680033402.01.T01 MTR_2g086450 38.043 92 49 3 154 244 16 100 4.82e-11 64.3
MsG0680033402.01.T01 MTR_4g098870 45.455 55 30 0 189 243 329 383 4.91e-11 64.7
MsG0680033402.01.T01 MTR_4g098870 45.455 55 30 0 189 243 309 363 5.08e-11 64.7
MsG0680033402.01.T01 MTR_4g098870 45.455 55 30 0 189 243 329 383 5.34e-11 64.7
MsG0680033402.01.T01 MTR_4g098870 45.455 55 30 0 189 243 348 402 5.55e-11 64.7
MsG0680033402.01.T01 MTR_6g032990 48.214 56 28 1 190 244 42 97 7.42e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033402.01.T01 AT2G01760 38.996 259 121 6 32 253 6 264 2.31e-50 174
MsG0680033402.01.T01 AT2G01760 35.192 287 121 7 32 253 6 292 1.70e-44 159
MsG0680033402.01.T01 AT1G67710 39.044 251 121 7 36 255 10 259 3.67e-44 160
MsG0680033402.01.T01 AT3G16857 34.909 275 130 6 27 253 27 300 3.88e-43 159
MsG0680033402.01.T01 AT3G16857 34.909 275 130 6 27 253 27 300 4.80e-43 159
MsG0680033402.01.T01 AT2G25180 32.558 258 148 3 20 252 1 257 3.20e-37 142
MsG0680033402.01.T01 AT4G31920 34.524 252 150 5 20 257 1 251 7.48e-37 140
MsG0680033402.01.T01 AT3G16857 33.913 230 105 4 71 253 1 230 4.11e-35 136
MsG0680033402.01.T01 AT5G58080 32.927 246 140 4 32 253 13 257 1.67e-33 132
MsG0680033402.01.T01 AT2G01760 38.265 196 84 6 95 253 4 199 2.68e-33 127
MsG0680033402.01.T01 AT1G67710 37.113 194 92 5 92 255 1 194 1.24e-30 122
MsG0680033402.01.T01 AT5G49240 30.364 247 142 6 22 239 27 272 1.24e-28 113
MsG0680033402.01.T01 AT3G62670 29.502 261 151 8 16 248 19 274 2.57e-28 115
MsG0680033402.01.T01 AT1G49190 26.217 267 156 6 23 253 20 281 1.08e-24 104
MsG0680033402.01.T01 AT1G67710 37.931 174 78 5 112 255 12 185 1.10e-24 105
MsG0680033402.01.T01 AT5G07210 26.394 269 141 6 37 250 16 282 1.37e-19 90.9
MsG0680033402.01.T01 AT5G07210 25.899 278 139 8 37 250 16 290 8.32e-18 85.5
MsG0680033402.01.T01 AT2G27070 25.468 267 143 6 37 247 16 282 5.72e-15 77.0
MsG0680033402.01.T01 AT3G46640 50.704 71 32 1 189 259 143 210 1.38e-14 74.3
MsG0680033402.01.T01 AT3G46640 50.704 71 32 1 189 259 143 210 1.38e-14 74.3
MsG0680033402.01.T01 AT5G59570 50.769 65 32 0 188 252 139 203 1.62e-14 73.9
MsG0680033402.01.T01 AT5G59570 50.769 65 32 0 188 252 139 203 1.62e-14 73.9
MsG0680033402.01.T01 AT3G46640 50.704 71 32 1 189 259 143 210 1.82e-14 74.3
MsG0680033402.01.T01 AT1G49190 28.148 135 97 0 23 157 20 154 4.37e-14 74.3
MsG0680033402.01.T01 AT2G40970 52.542 59 28 0 189 247 104 162 5.62e-14 71.6
MsG0680033402.01.T01 AT5G05090 54.237 59 27 0 189 247 80 138 5.90e-14 72.0
MsG0680033402.01.T01 AT3G10760 54.237 59 27 0 189 247 104 162 7.32e-14 72.4
MsG0680033402.01.T01 AT3G04030 53.571 56 25 1 190 244 45 100 7.41e-12 66.6
MsG0680033402.01.T01 AT3G04030 53.571 56 25 1 190 244 45 100 8.22e-12 66.6
MsG0680033402.01.T01 AT3G04030 50.909 55 27 0 190 244 45 99 9.55e-12 66.6
MsG0680033402.01.T01 AT5G18240 53.571 56 25 1 190 244 45 100 1.08e-11 66.2
MsG0680033402.01.T01 AT5G18240 53.571 56 25 1 190 244 45 100 1.08e-11 66.2
MsG0680033402.01.T01 AT5G18240 53.571 56 25 1 190 244 45 100 1.10e-11 66.2
MsG0680033402.01.T01 AT5G18240 53.571 56 25 1 190 244 45 100 1.16e-11 66.2
MsG0680033402.01.T01 AT5G18240 53.571 56 25 1 190 244 45 100 1.16e-11 66.2
MsG0680033402.01.T01 AT2G46790 32.836 134 84 3 37 164 37 170 5.33e-11 64.3
MsG0680033402.01.T01 AT2G46790 32.836 134 84 3 37 164 37 170 6.39e-11 63.9
MsG0680033402.01.T01 AT5G44190 40.000 75 40 1 189 263 146 215 7.06e-11 63.5
MsG0680033402.01.T01 AT5G44190 46.296 54 29 0 189 242 146 199 7.16e-11 63.5

Find 54 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAATTCTTAGAGGGTTTA+AGG 0.118099 6:+64094753 MsG0680033402.01.T01:CDS
CTAAGGGCTGTGAATCAATT+TGG 0.229550 6:+64095670 MsG0680033402.01.T01:CDS
ACAACAACATTCATCAAAAT+TGG 0.275016 6:+64097750 MsG0680033402.01.T01:CDS
CAATGCATCAAGAATTTCTA+AGG 0.275756 6:+64095653 MsG0680033402.01.T01:CDS
GCATATTGCTAGCCATTTAC+AGG 0.279083 6:+64095850 MsG0680033402.01.T01:CDS
TGCTTTAATCCCTTCTTCAA+TGG 0.341450 6:+64094700 MsG0680033402.01.T01:CDS
CCCGTTTGCAATAGCCTTCA+AGG 0.349006 6:-64095431 None:intergenic
ACTTCCCCAACAATTCTTAG+AGG 0.375785 6:+64094745 MsG0680033402.01.T01:CDS
TGACAGGAATATTGATTTGT+TGG 0.378211 6:-64095101 None:intergenic
GATCAAATTCATGTTGATAA+TGG 0.381612 6:+64095547 MsG0680033402.01.T01:CDS
TAAACCCTCTAAGAATTGTT+GGG 0.382270 6:-64094750 None:intergenic
ACAATTCTTAGAGGGTTTAA+GGG 0.385698 6:+64094754 MsG0680033402.01.T01:CDS
TGATTTGTTGGGTAATTTGT+TGG 0.402510 6:-64095089 None:intergenic
ATTCAAGTGATGAATGTTCC+TGG 0.409770 6:+64095815 MsG0680033402.01.T01:CDS
CCTTGAAGGCTATTGCAAAC+GGG 0.420675 6:+64095431 MsG0680033402.01.T01:CDS
TTAAACCCTCTAAGAATTGT+TGG 0.427205 6:-64094751 None:intergenic
GCCATCGTTGAAAAGTCAAT+TGG 0.430946 6:+64094812 MsG0680033402.01.T01:CDS
TCCTTGAAGGCTATTGCAAA+CGG 0.433944 6:+64095430 MsG0680033402.01.T01:CDS
GACAGGAATATTGATTTGTT+GGG 0.435755 6:-64095100 None:intergenic
TGTGCAGTGATGGGTGTTGA+TGG 0.436154 6:+64095394 MsG0680033402.01.T01:intron
AATTTGTTGGAGAAAATCAT+AGG 0.440238 6:-64095076 None:intergenic
AACGATGGCATTGTGGATAG+TGG 0.451069 6:-64094798 None:intergenic
AATTGAAACTTACTTTAAAG+AGG 0.455987 6:+64097569 MsG0680033402.01.T01:CDS
TTATTGTCACATTGTATGAC+TGG 0.461320 6:-64097715 None:intergenic
TTCAAAACATTAGACTGATC+AGG 0.466345 6:-64097784 None:intergenic
ATCAACAAGTGCATCCTTGA+AGG 0.472077 6:+64095417 MsG0680033402.01.T01:CDS
GTTGAAAGAGCCATTGAAGA+AGG 0.472839 6:-64094710 None:intergenic
GGCAAAGAATCTTACTATCA+AGG 0.473824 6:-64095693 None:intergenic
AGACTGATCAGTAAAATTGT+TGG 0.484727 6:-64097863 None:intergenic
ATGAATGTTGTTGTGGTGCA+TGG 0.489060 6:-64097741 None:intergenic
AATGGCTCTTTCAACTGAGA+TGG 0.494149 6:+64094718 MsG0680033402.01.T01:CDS
TCATGTTGATGTGGTTGAAA+AGG 0.515561 6:+64095582 MsG0680033402.01.T01:CDS
CGTTGAAAAGTCAATTGGAT+GGG 0.523926 6:+64094817 MsG0680033402.01.T01:CDS
TTAAAGAATGAAATACCAAA+AGG 0.536683 6:+64097610 MsG0680033402.01.T01:CDS
TATATGTTCTTACTAATGAC+AGG 0.542103 6:-64095117 None:intergenic
CTCATCACCTGGATTCATGT+TGG 0.553676 6:-64097974 None:intergenic
CTTCCCCAACAATTCTTAGA+GGG 0.553790 6:+64094746 MsG0680033402.01.T01:CDS
CAATTAAAGAACTCATCACC+TGG 0.565714 6:-64097985 None:intergenic
TTGAAAGAGCCATTGAAGAA+GGG 0.568093 6:-64094709 None:intergenic
TTGATTAGGTCATCACTCAA+AGG 0.569533 6:-64095475 None:intergenic
GATCAGTCCAACATGAATCC+AGG 0.574425 6:+64097967 MsG0680033402.01.T01:CDS
TCGTTGAAAAGTCAATTGGA+TGG 0.580260 6:+64094816 MsG0680033402.01.T01:CDS
ATGAAATACCAAAAGGTGAG+TGG 0.583760 6:+64097617 MsG0680033402.01.T01:CDS
TTCACTCTTCACCTCAACAA+CGG 0.596457 6:+64094675 MsG0680033402.01.T01:CDS
TCAATTGGATGGGATTACAA+AGG 0.596849 6:+64094827 MsG0680033402.01.T01:CDS
AAACCCTCTAAGAATTGTTG+GGG 0.603847 6:-64094749 None:intergenic
CTTGAAGGCTATTGCAAACG+GGG 0.604122 6:+64095432 MsG0680033402.01.T01:CDS
ACATGTGCAAACACGTTCGA+AGG 0.611418 6:+64095500 MsG0680033402.01.T01:CDS
AAAAGAAGCATTAGAGCATG+TGG 0.613637 6:-64094977 None:intergenic
AATGCATCAAGAATTTCTAA+GGG 0.619626 6:+64095654 MsG0680033402.01.T01:CDS
ATTAAAGCAAACCGTTGTTG+AGG 0.620227 6:-64094686 None:intergenic
AAAAGAATCTCATGTTGATG+TGG 0.621750 6:+64095573 MsG0680033402.01.T01:CDS
GTGAATGTCGGTGAATGTTG+TGG 0.625497 6:-64097931 None:intergenic
ATATGCTCTCTTGTCAAACC+AGG 0.644357 6:-64095833 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGCATTATTTAAAAAA+GGG - Chr6:64096844-64096863 None:intergenic 10.0%
!! TAAATTGCATTATTTAAAAA+AGG - Chr6:64096845-64096864 None:intergenic 10.0%
!! TCATTTAACTATAAATTTAA+GGG - Chr6:64097004-64097023 None:intergenic 10.0%
!!! AAAAAAAAAATATTTTACAG+GGG + Chr6:64096770-64096789 MsG0680033402.01.T01:intron 10.0%
!!! ATTCAAAAAAAATATTTTAC+AGG - Chr6:64096519-64096538 None:intergenic 10.0%
!!! CAAAAAAAAAATATTTTACA+GGG + Chr6:64096769-64096788 MsG0680033402.01.T01:intron 10.0%
!!! TTCAAAAAAAATATTTTACA+GGG - Chr6:64096518-64096537 None:intergenic 10.0%
!! AAAAGAAAGAAAAAAAAAAG+TGG - Chr6:64095717-64095736 None:intergenic 15.0%
!! ATAATAAAAAAAATAACACG+TGG + Chr6:64095993-64096012 MsG0680033402.01.T01:intron 15.0%
!! CTCATTTAACTATAAATTTA+AGG - Chr6:64097005-64097024 None:intergenic 15.0%
!! TTAATTGTGTACTAAAAAAA+AGG + Chr6:64096300-64096319 MsG0680033402.01.T01:intron 15.0%
!!! AAAAAAAAATATTTTACAGG+GGG + Chr6:64096771-64096790 MsG0680033402.01.T01:intron 15.0%
!!! AATGGTGTAATATATTTTTT+TGG + Chr6:64097366-64097385 MsG0680033402.01.T01:intron 15.0%
!!! ATCACTTGAATAATTTTTTT+TGG - Chr6:64095806-64095825 None:intergenic 15.0%
!!! ATGGTGTAATATATTTTTTT+GGG + Chr6:64097367-64097386 MsG0680033402.01.T01:intron 15.0%
!!! ATTGAAAATTTGATAGATTT+TGG + Chr6:64095961-64095980 MsG0680033402.01.T01:intron 15.0%
!!! CCAAAAAAAAAATATTTTAC+AGG + Chr6:64096768-64096787 MsG0680033402.01.T01:intron 15.0%
!!! CCTGTAAAATATTTTTTTTT+TGG - Chr6:64096771-64096790 None:intergenic 15.0%
!!! GTTTATGATGTTTTTTTAAA+GGG + Chr6:64096445-64096464 MsG0680033402.01.T01:intron 15.0%
!!! GTTTGTTTTTTAGATTAATT+TGG + Chr6:64095872-64095891 MsG0680033402.01.T01:intron 15.0%
!!! TCAAAAAAAATATTTTACAG+GGG - Chr6:64096517-64096536 None:intergenic 15.0%
!!! TTGAAAATTTGATAGATTTT+GGG + Chr6:64095962-64095981 MsG0680033402.01.T01:intron 15.0%
!! ATATTACACCATTGAAAATA+GGG - Chr6:64097359-64097378 None:intergenic 20.0%
!! ATCATAATAGAGAACAATAA+AGG - Chr6:64096877-64096896 None:intergenic 20.0%
!! CAAAAAATCTTCAAATTACA+AGG - Chr6:64096547-64096566 None:intergenic 20.0%
!! GATCGTTAATTTATAGATTT+AGG + Chr6:64097285-64097304 MsG0680033402.01.T01:intron 20.0%
!! TATATTACACCATTGAAAAT+AGG - Chr6:64097360-64097379 None:intergenic 20.0%
!! TTAAAGAATGAAATACCAAA+AGG + Chr6:64097610-64097629 MsG0680033402.01.T01:CDS 20.0%
!!! AAAAAAAATATTTTACAGGG+GGG - Chr6:64096791-64096772 None:intergenic 20.0%
!!! AAATTTGCTCAAATTAAAAG+GGG - Chr6:64096659-64096678 None:intergenic 20.0%
!!! AATTGAAACTTACTTTAAAG+AGG + Chr6:64097569-64097588 MsG0680033402.01.T01:CDS 20.0%
!!! AATTTGAAGATTTTTGGTTT+TGG - Chr6:64096735-64096754 None:intergenic 20.0%
!!! ATTTGAAGATTTTTTGGTTT+TGG + Chr6:64096551-64096570 MsG0680033402.01.T01:intron 20.0%
!!! CAAAAAAAATATTTTACAGG+GGG - Chr6:64096516-64096535 None:intergenic 20.0%
!!! CTTGTAATTTGAAGATTTTT+TGG + Chr6:64096545-64096564 MsG0680033402.01.T01:intron 20.0%
!!! CTTTTAATTTGAGCAAATTT+CGG + Chr6:64096659-64096678 MsG0680033402.01.T01:intron 20.0%
!!! GAAATTTGCTCAAATTAAAA+GGG - Chr6:64096660-64096679 None:intergenic 20.0%
!!! GGTTTATGATGTTTTTTTAA+AGG + Chr6:64096444-64096463 MsG0680033402.01.T01:intron 20.0%
!!! GTTTTGTAGTTTTTTTCTTA+GGG + Chr6:64096105-64096124 MsG0680033402.01.T01:intron 20.0%
!!! TGGTGTAATATATTTTTTTG+GGG + Chr6:64097368-64097387 MsG0680033402.01.T01:intron 20.0%
!!! TTTTGTAGTTTTTTTCTTAG+GGG + Chr6:64096106-64096125 MsG0680033402.01.T01:intron 20.0%
! AATGCATCAAGAATTTCTAA+GGG + Chr6:64095654-64095673 MsG0680033402.01.T01:CDS 25.0%
! AATTGATACACCAAAAGAAT+TGG + Chr6:64096398-64096417 MsG0680033402.01.T01:intron 25.0%
! ACAACAACATTCATCAAAAT+TGG + Chr6:64097750-64097769 MsG0680033402.01.T01:CDS 25.0%
! ACTGTACAAAATCGAAAATA+TGG - Chr6:64096696-64096715 None:intergenic 25.0%
! CAAAAAAGAACCGTTTAAAA+TGG + Chr6:64095623-64095642 MsG0680033402.01.T01:CDS 25.0%
! CCAAAAATCTTCAAATTACA+AGG + Chr6:64096738-64096757 MsG0680033402.01.T01:intron 25.0%
! CTAAAAAACAAACCTGTAAA+TGG - Chr6:64095865-64095884 None:intergenic 25.0%
! GTAAAAATATTGGTGAATGT+CGG - Chr6:64097946-64097965 None:intergenic 25.0%
! TATATGTTCTTACTAATGAC+AGG - Chr6:64095120-64095139 None:intergenic 25.0%
! TGAATGTATGTTATTAGCAA+AGG + Chr6:64096228-64096247 MsG0680033402.01.T01:intron 25.0%
! TGGAAATGACTTATATTACA+GGG - Chr6:64096487-64096506 None:intergenic 25.0%
!! AAATTTGCTCAAATTACAAG+GGG - Chr6:64096627-64096646 None:intergenic 25.0%
!! AATTTGCTCAAATTAAAAGG+GGG - Chr6:64096658-64096677 None:intergenic 25.0%
!! ATAGGCATATGCTTAATTTT+TGG - Chr6:64096181-64096200 None:intergenic 25.0%
!! CCTTGTAATTTGAAGATTTT+TGG - Chr6:64096563-64096544 None:intergenic 25.0%
!! CGAAATTTGCTCAAATTAAA+AGG - Chr6:64096661-64096680 None:intergenic 25.0%
!! CTAAATTTGCTCAAATTACA+GGG - Chr6:64096598-64096617 None:intergenic 25.0%
!! GAAATTTGCTCAAATTACAA+GGG - Chr6:64096628-64096647 None:intergenic 25.0%
!! GATCAAATTCATGTTGATAA+TGG + Chr6:64095547-64095566 MsG0680033402.01.T01:CDS 25.0%
!! TAAATTTGCTCAAATTACAG+GGG - Chr6:64096597-64096616 None:intergenic 25.0%
!!! AATGTCTGTTTTTGCTTTAT+TGG + Chr6:64095240-64095259 MsG0680033402.01.T01:intron 25.0%
!!! AATTTGTTGGAGAAAATCAT+AGG - Chr6:64095079-64095098 None:intergenic 25.0%
!!! AATTTTGATGAATGTTGTTG+TGG - Chr6:64097751-64097770 None:intergenic 25.0%
!!! GGTTTTGTAGTTTTTTTCTT+AGG + Chr6:64096104-64096123 MsG0680033402.01.T01:intron 25.0%
!!! GTTTTGCATCTGAAATATAA+GGG - Chr6:64095784-64095803 None:intergenic 25.0%
!!! TTAAACGGTTCTTTTTTGTT+GGG - Chr6:64095621-64095640 None:intergenic 25.0%
!!! TTAACATTTTGATCAAGTTC+TGG - Chr6:64097691-64097710 None:intergenic 25.0%
!!! TTTAAACGGTTCTTTTTTGT+TGG - Chr6:64095622-64095641 None:intergenic 25.0%
!!! TTTGTAGTTTTTTTCTTAGG+GGG + Chr6:64096107-64096126 MsG0680033402.01.T01:intron 25.0%
AAAAGAATCTCATGTTGATG+TGG + Chr6:64095573-64095592 MsG0680033402.01.T01:CDS 30.0%
AACAATTCTTAGAGGGTTTA+AGG + Chr6:64094753-64094772 MsG0680033402.01.T01:CDS 30.0%
AATAGAGAACAATAAAGGTC+AGG - Chr6:64096872-64096891 None:intergenic 30.0%
ACAAAATCGAAAATATGGCA+GGG - Chr6:64096691-64096710 None:intergenic 30.0%
ACAATTCTTAGAGGGTTTAA+GGG + Chr6:64094754-64094773 MsG0680033402.01.T01:CDS 30.0%
ACTCAGATATATTGAGTGTA+CGG - Chr6:64096965-64096984 None:intergenic 30.0%
AGAAATGACCTATATTACAG+GGG + Chr6:64096801-64096820 MsG0680033402.01.T01:intron 30.0%
AGACTGATCAGTAAAATTGT+TGG - Chr6:64097866-64097885 None:intergenic 30.0%
ATTTGCTCAAATTAAAAGGG+GGG - Chr6:64096657-64096676 None:intergenic 30.0%
CAATGCATCAAGAATTTCTA+AGG + Chr6:64095653-64095672 MsG0680033402.01.T01:CDS 30.0%
CAGAAATGACCTATATTACA+GGG + Chr6:64096800-64096819 MsG0680033402.01.T01:intron 30.0%
CCTGTAATTTGAGCAAATTT+AGG + Chr6:64096596-64096615 MsG0680033402.01.T01:intron 30.0%
CTGGAAATGACTTATATTAC+AGG - Chr6:64096488-64096507 None:intergenic 30.0%
GAAATGACTTATATTACAGG+GGG - Chr6:64096485-64096504 None:intergenic 30.0%
GCTCATATATTGATAAAGAG+AGG + Chr6:64096423-64096442 MsG0680033402.01.T01:intron 30.0%
GGAAATGACTTATATTACAG+GGG - Chr6:64096486-64096505 None:intergenic 30.0%
GGACTGATCAGTAAAAATAT+TGG - Chr6:64097956-64097975 None:intergenic 30.0%
TACAAAATCGAAAATATGGC+AGG - Chr6:64096692-64096711 None:intergenic 30.0%
TCAGTCTAGGAAATATTGAT+TGG + Chr6:64096372-64096391 MsG0680033402.01.T01:intron 30.0%
TGAGTGGAAAAAATTAAAGC+AGG + Chr6:64097633-64097652 MsG0680033402.01.T01:CDS 30.0%
TGTACAGTCAAAATTCATGA+AGG + Chr6:64096708-64096727 MsG0680033402.01.T01:intron 30.0%
TTATTGTCACATTGTATGAC+TGG - Chr6:64097718-64097737 None:intergenic 30.0%
TTCAAAACATTAGACTGATC+AGG - Chr6:64097787-64097806 None:intergenic 30.0%
! AAATTTGCTCAAATTACAGG+GGG - Chr6:64096596-64096615 None:intergenic 30.0%
! AATTTGCTCAAATTACAAGG+GGG - Chr6:64096626-64096645 None:intergenic 30.0%
! CCTAAATTTGCTCAAATTAC+AGG - Chr6:64096599-64096618 None:intergenic 30.0%
! CGAAATTTGCTCAAATTACA+AGG - Chr6:64096629-64096648 None:intergenic 30.0%
! TAAACCCTCTAAGAATTGTT+GGG - Chr6:64094753-64094772 None:intergenic 30.0%
! TTAAACCCTCTAAGAATTGT+TGG - Chr6:64094754-64094773 None:intergenic 30.0%
! TTGCTTGAGACCATTTTAAA+CGG - Chr6:64095636-64095655 None:intergenic 30.0%
!! GACAGGAATATTGATTTGTT+GGG - Chr6:64095103-64095122 None:intergenic 30.0%
!! GGTTTTGCATCTGAAATATA+AGG - Chr6:64095785-64095804 None:intergenic 30.0%
!! GTTTGCACATGTTTTTGATT+AGG - Chr6:64095492-64095511 None:intergenic 30.0%
!! TGACAGGAATATTGATTTGT+TGG - Chr6:64095104-64095123 None:intergenic 30.0%
!! TGATTTGTTGGGTAATTTGT+TGG - Chr6:64095092-64095111 None:intergenic 30.0%
!! TTTTATTTCGTTGACGCTTT+TGG - Chr6:64097092-64097111 None:intergenic 30.0%
!!! AATTTTTTCCACTCACCTTT+TGG - Chr6:64097628-64097647 None:intergenic 30.0%
!!! ACGACTTTATGTTATTTTCG+AGG + Chr6:64096930-64096949 MsG0680033402.01.T01:intron 30.0%
!!! TAAACGGTTCTTTTTTGTTG+GGG - Chr6:64095620-64095639 None:intergenic 30.0%
!!! TTTTGCATTGACTCTTTTGA+GGG + Chr6:64094996-64095015 MsG0680033402.01.T01:CDS 30.0%
AAAATCGAAAATATGGCAGG+GGG - Chr6:64096689-64096708 None:intergenic 35.0%
AAATGACCTATATTACAGGG+GGG + Chr6:64096803-64096822 MsG0680033402.01.T01:intron 35.0%
AATTTAAGGGTTTGATGTGC+TGG - Chr6:64096991-64097010 None:intergenic 35.0%
AGACTGATCAGGAAAAATGT+TGG - Chr6:64097776-64097795 None:intergenic 35.0%
ATGAAATACCAAAAGGTGAG+TGG + Chr6:64097617-64097636 MsG0680033402.01.T01:CDS 35.0%
ATTAAAGCAAACCGTTGTTG+AGG - Chr6:64094689-64094708 None:intergenic 35.0%
ATTCAAGTGATGAATGTTCC+TGG + Chr6:64095815-64095834 MsG0680033402.01.T01:CDS 35.0%
CAAAATCGAAAATATGGCAG+GGG - Chr6:64096690-64096709 None:intergenic 35.0%
CAATTAAAGAACTCATCACC+TGG - Chr6:64097988-64098007 None:intergenic 35.0%
CCAGAAATGACCTATATTAC+AGG + Chr6:64096799-64096818 MsG0680033402.01.T01:intron 35.0%
CCTGTAATATAGGTCATTTC+TGG - Chr6:64096802-64096821 None:intergenic 35.0%
CGTTGAAAAGTCAATTGGAT+GGG + Chr6:64094817-64094836 MsG0680033402.01.T01:CDS 35.0%
GAAATGACCTATATTACAGG+GGG + Chr6:64096802-64096821 MsG0680033402.01.T01:intron 35.0%
GCAAACATTAGTGCGTATAT+AGG - Chr6:64096199-64096218 None:intergenic 35.0%
GGCAAAGAATCTTACTATCA+AGG - Chr6:64095696-64095715 None:intergenic 35.0%
TCAATTGGATGGGATTACAA+AGG + Chr6:64094827-64094846 MsG0680033402.01.T01:CDS 35.0%
TCATGTTGATGTGGTTGAAA+AGG + Chr6:64095582-64095601 MsG0680033402.01.T01:CDS 35.0%
TCGTTGAAAAGTCAATTGGA+TGG + Chr6:64094816-64094835 MsG0680033402.01.T01:CDS 35.0%
TGATACACCAAAAGAATTGG+TGG + Chr6:64096401-64096420 MsG0680033402.01.T01:intron 35.0%
TGCTTTAATCCCTTCTTCAA+TGG + Chr6:64094700-64094719 MsG0680033402.01.T01:CDS 35.0%
TTGAAAGAGCCATTGAAGAA+GGG - Chr6:64094712-64094731 None:intergenic 35.0%
TTGATTAGGTCATCACTCAA+AGG - Chr6:64095478-64095497 None:intergenic 35.0%
! AAACCCTCTAAGAATTGTTG+GGG - Chr6:64094752-64094771 None:intergenic 35.0%
! ATATGAGCCACCAATTCTTT+TGG - Chr6:64096411-64096430 None:intergenic 35.0%
! GGGGGTGAAATTTTAAATGA+AGG - Chr6:64096578-64096597 None:intergenic 35.0%
! GTCAAAAGCGAAAGTGATTT+TGG - Chr6:64095212-64095231 None:intergenic 35.0%
! TAGAAGCTCCCTATTTTCAA+TGG + Chr6:64097348-64097367 MsG0680033402.01.T01:intron 35.0%
! TGCATCTGAAATATAAGGGA+GGG - Chr6:64095780-64095799 None:intergenic 35.0%
! TTGCATCTGAAATATAAGGG+AGG - Chr6:64095781-64095800 None:intergenic 35.0%
!! AAAAGAAGCATTAGAGCATG+TGG - Chr6:64094980-64094999 None:intergenic 35.0%
!! TCCAATTGACTTTTCAACGA+TGG - Chr6:64094816-64094835 None:intergenic 35.0%
!!! AAACGGTTCTTTTTTGTTGG+GGG - Chr6:64095619-64095638 None:intergenic 35.0%
!!! CTTTTGCATTGACTCTTTTG+AGG + Chr6:64094995-64095014 MsG0680033402.01.T01:CDS 35.0%
!!! TCTGTTTTTGCTTTATTGGC+TGG + Chr6:64095244-64095263 MsG0680033402.01.T01:intron 35.0%
AATGACCTATATTACAGGGG+GGG + Chr6:64096804-64096823 MsG0680033402.01.T01:intron 40.0%
AATGGCTCTTTCAACTGAGA+TGG + Chr6:64094718-64094737 MsG0680033402.01.T01:CDS 40.0%
ACTTCCCCAACAATTCTTAG+AGG + Chr6:64094745-64094764 MsG0680033402.01.T01:CDS 40.0%
ATATGCTCTCTTGTCAAACC+AGG - Chr6:64095836-64095855 None:intergenic 40.0%
ATATGTGTCATGTGCAGTGA+TGG + Chr6:64095384-64095403 MsG0680033402.01.T01:intron 40.0%
ATCAACAAGTGCATCCTTGA+AGG + Chr6:64095417-64095436 MsG0680033402.01.T01:CDS 40.0%
ATCGCAACTTCGTGAAAGTT+AGG + Chr6:64096135-64096154 MsG0680033402.01.T01:intron 40.0%
ATGAATGTTGTTGTGGTGCA+TGG - Chr6:64097744-64097763 None:intergenic 40.0%
CCTTAGGTGCAAAACTTAGA+AGG + Chr6:64096080-64096099 MsG0680033402.01.T01:intron 40.0%
CTAAGGGCTGTGAATCAATT+TGG + Chr6:64095670-64095689 MsG0680033402.01.T01:CDS 40.0%
CTTCCCCAACAATTCTTAGA+GGG + Chr6:64094746-64094765 MsG0680033402.01.T01:CDS 40.0%
GAATGCACATCAATCAGTCT+AGG + Chr6:64096359-64096378 MsG0680033402.01.T01:intron 40.0%
GCATATTGCTAGCCATTTAC+AGG + Chr6:64095850-64095869 MsG0680033402.01.T01:CDS 40.0%
GCCATCGTTGAAAAGTCAAT+TGG + Chr6:64094812-64094831 MsG0680033402.01.T01:CDS 40.0%
GTTGAAAGAGCCATTGAAGA+AGG - Chr6:64094713-64094732 None:intergenic 40.0%
TACGATACACTCGTAAGTTG+AGG - Chr6:64097330-64097349 None:intergenic 40.0%
TAGGTGCAAAACTTAGAAGG+CGG + Chr6:64096083-64096102 MsG0680033402.01.T01:intron 40.0%
TATGTGTCATGTGCAGTGAT+GGG + Chr6:64095385-64095404 MsG0680033402.01.T01:intron 40.0%
TCCTTGAAGGCTATTGCAAA+CGG + Chr6:64095430-64095449 MsG0680033402.01.T01:CDS 40.0%
TCGCAACTTCGTGAAAGTTA+GGG + Chr6:64096136-64096155 MsG0680033402.01.T01:intron 40.0%
TTCACTCTTCACCTCAACAA+CGG + Chr6:64094675-64094694 MsG0680033402.01.T01:CDS 40.0%
! ACTCGTAAGTTGAGGTGTAT+CGG - Chr6:64097322-64097341 None:intergenic 40.0%
! ACTTTTCAACGATGGCATTG+TGG - Chr6:64094808-64094827 None:intergenic 40.0%
! CCTTCTAAGTTTTGCACCTA+AGG - Chr6:64096083-64096102 None:intergenic 40.0%
! CTTTCACGAAGTTGCGATTT+TGG - Chr6:64096133-64096152 None:intergenic 40.0%
! GCATCTGAAATATAAGGGAG+GGG - Chr6:64095779-64095798 None:intergenic 40.0%
! TATTTTACAGGGGGGAAAAC+TGG - Chr6:64096507-64096526 None:intergenic 40.0%
! TTTTACCCCCCCTGTAATAT+AGG - Chr6:64096812-64096831 None:intergenic 40.0%
! TTTTGCACCTAAGGCAGTTT+TGG - Chr6:64096074-64096093 None:intergenic 40.0%
!! ACTCTTTTGAGGGAAGCAAA+GGG + Chr6:64095006-64095025 MsG0680033402.01.T01:CDS 40.0%
AACGATGGCATTGTGGATAG+TGG - Chr6:64094801-64094820 None:intergenic 45.0%
ACATGTGCAAACACGTTCGA+AGG + Chr6:64095500-64095519 MsG0680033402.01.T01:CDS 45.0%
ATGTCAAATCAGCGTAGACC+GGG + Chr6:64096029-64096048 MsG0680033402.01.T01:intron 45.0%
CAATCAGTCACTCAAGCACA+AGG - Chr6:64095320-64095339 None:intergenic 45.0%
CACACACACTTATGTGCAAC+AGG + Chr6:64095178-64095197 MsG0680033402.01.T01:intron 45.0%
CCTTGAAGGCTATTGCAAAC+GGG + Chr6:64095431-64095450 MsG0680033402.01.T01:CDS 45.0%
CGCAACTTCGTGAAAGTTAG+GGG + Chr6:64096137-64096156 MsG0680033402.01.T01:intron 45.0%
CTCATCACCTGGATTCATGT+TGG - Chr6:64097977-64097996 None:intergenic 45.0%
CTTGAAGGCTATTGCAAACG+GGG + Chr6:64095432-64095451 MsG0680033402.01.T01:CDS 45.0%
GATCAGTCCAACATGAATCC+AGG + Chr6:64097967-64097986 MsG0680033402.01.T01:CDS 45.0%
GATGTCAAATCAGCGTAGAC+CGG + Chr6:64096028-64096047 MsG0680033402.01.T01:intron 45.0%
GCAACTTCGTGAAAGTTAGG+GGG + Chr6:64096138-64096157 MsG0680033402.01.T01:intron 45.0%
GCATTAGAGCATGTGGTAAC+TGG - Chr6:64094973-64094992 None:intergenic 45.0%
GTGAATGTCGGTGAATGTTG+TGG - Chr6:64097934-64097953 None:intergenic 45.0%
! CTGATTTGACATCCTCAGTG+AGG - Chr6:64096021-64096040 None:intergenic 45.0%
!! GACTCTTTTGAGGGAAGCAA+AGG + Chr6:64095005-64095024 MsG0680033402.01.T01:CDS 45.0%
!! AATAATATAATTAATTATGT+AGG - Chr6:64095147-64095166 None:intergenic 5.0%
ACCGGGTCAAAAATGGCCTT+GGG + Chr6:64096046-64096065 MsG0680033402.01.T01:intron 50.0%
AGCGTAGACCGGGTCAAAAA+TGG + Chr6:64096039-64096058 MsG0680033402.01.T01:intron 50.0%
CCCGTTTGCAATAGCCTTCA+AGG - Chr6:64095434-64095453 None:intergenic 50.0%
!! TGTGCAGTGATGGGTGTTGA+TGG + Chr6:64095394-64095413 MsG0680033402.01.T01:intron 50.0%
CAAGCACAAGGCGTGCTGAA+GGG - Chr6:64095308-64095327 None:intergenic 55.0%
GACCGGGTCAAAAATGGCCT+TGG + Chr6:64096045-64096064 MsG0680033402.01.T01:intron 55.0%
TAACACGTGGCACCTCACTG+AGG + Chr6:64096006-64096025 MsG0680033402.01.T01:intron 55.0%
TCAAGCACAAGGCGTGCTGA+AGG - Chr6:64095309-64095328 None:intergenic 55.0%
TTGGGGGCCAAAACTGCCTT+AGG + Chr6:64096064-64096083 MsG0680033402.01.T01:intron 55.0%
! CCGGGTCAAAAATGGCCTTG+GGG + Chr6:64096047-64096066 MsG0680033402.01.T01:intron 55.0%
! CGGGTCAAAAATGGCCTTGG+GGG + Chr6:64096048-64096067 MsG0680033402.01.T01:intron 55.0%
!! TAAGGCAGTTTTGGCCCCCA+AGG - Chr6:64096065-64096084 None:intergenic 55.0%
!!! CCCCAAGGCCATTTTTGACC+CGG - Chr6:64096050-64096069 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 64094662 64098008 64094662 ID=MsG0680033402.01;Name=MsG0680033402.01
Chr6 mRNA 64094662 64098008 64094662 ID=MsG0680033402.01.T01;Parent=MsG0680033402.01;Name=MsG0680033402.01.T01;_AED=0.08;_eAED=0.09;_QI=0|0|0|1|1|1|5|0|385
Chr6 exon 64094662 64094848 64094662 ID=MsG0680033402.01.T01:exon:4044;Parent=MsG0680033402.01.T01
Chr6 exon 64094974 64095126 64094974 ID=MsG0680033402.01.T01:exon:4043;Parent=MsG0680033402.01.T01
Chr6 exon 64095401 64095700 64095401 ID=MsG0680033402.01.T01:exon:4042;Parent=MsG0680033402.01.T01
Chr6 exon 64095795 64095871 64095795 ID=MsG0680033402.01.T01:exon:4041;Parent=MsG0680033402.01.T01
Chr6 exon 64097568 64098008 64097568 ID=MsG0680033402.01.T01:exon:4040;Parent=MsG0680033402.01.T01
Chr6 CDS 64094662 64094848 64094662 ID=MsG0680033402.01.T01:cds;Parent=MsG0680033402.01.T01
Chr6 CDS 64094974 64095126 64094974 ID=MsG0680033402.01.T01:cds;Parent=MsG0680033402.01.T01
Chr6 CDS 64095401 64095700 64095401 ID=MsG0680033402.01.T01:cds;Parent=MsG0680033402.01.T01
Chr6 CDS 64095795 64095871 64095795 ID=MsG0680033402.01.T01:cds;Parent=MsG0680033402.01.T01
Chr6 CDS 64097568 64098008 64097568 ID=MsG0680033402.01.T01:cds;Parent=MsG0680033402.01.T01
Gene Sequence

>MsG0680033402.01.T01

ATGTTTCTTGAGTTTCACTCTTCACCTCAACAACGGTTTGCTTTAATCCCTTCTTCAATGGCTCTTTCAACTGAGATGGAAGCACTTCCCCAACAATTCTTAGAGGGTTTAAGGGTTCTTGTGATTGATCATGACACCACTATCCACAATGCCATCGTTGAAAAGTCAATTGGATGGGATTACAAAGTTACCACATGCTCTAATGCTTCTTTTGCATTGACTCTTTTGAGGGAAGCAAAGGGTTGTTTTGATGTGATACTAATTGAAGAACAAATCTCAGACATGAATTCCTATGATTTTCTCCAACAAATTACCCAACAAATCAATATTCCTGTCATTATGATGGGTGTTGATGGATCAACAAGTGCATCCTTGAAGGCTATTGCAAACGGGGCTTGTGAGTATTGTGTTAAGCCTTTGAGTGATGACCTAATCAAAAACATGTGCAAACACGTTCGAAGGAAAACTTTGAATGAAACTAAACATGATCAAATTCATGTTGATAATGGCACAAAAGAATCTCATGTTGATGTGGTTGAAAAGGATAATTATCAACCCCCAACAAAAAAGAACCGTTTAAAATGGTCTCAAGCAATGCATCAAGAATTTCTAAGGGCTGTGAATCAATTTGGCCTTGATAATGCAAAACCAAAAAAAATTATTCAAGTGATGAATGTTCCTGGTTTGACAAGAGAGCATATTGCTAGCCATTTACAGAAATTGAAACTTACTTTAAAGAGGTCAAATGAAGTGACACAGTTAAAGAATGAAATACCAAAAGGTGAGTGGAAAAAATTAAAGCAGGATCAATGTCATCTTGAAGCTGCTAAGTCAACGCCAGAACTTGATCAAAATGTTAAAAATCCAGTCATACAATGTGACAATAATTTCCATGCACCACAACAACATTCATCAAAATTGGCCAACATTTTTCCTGATCAGTCTAATGTTTTGAATTCGATCATACAATATGACAATAATTATCATGCACTACAACATTCACTGACATTTACCAACAATTTTACTGATCAGTCTAGTATTCTAAATTCAGTCATACAACGTGTCAATAATTCTCATGCACCACAACATTCACCGACATTCACCAATATTTTTACTGATCAGTCCAACATGAATCCAGGTGATGAGTTCTTTAATTGA
Protein sequence

>MsG0680033402.01.T01

MFLEFHSSPQQRFALIPSSMALSTEMEALPQQFLEGLRVLVIDHDTTIHNAIVEKSIGWDYKVTTCSNASFALTLLREAKGCFDVILIEEQISDMNSYDFLQQITQQINIPVIMMGVDGSTSASLKAIANGACEYCVKPLSDDLIKNMCKHVRRKTLNETKHDQIHVDNGTKESHVDVVEKDNYQPPTKKNRLKWSQAMHQEFLRAVNQFGLDNAKPKKIIQVMNVPGLTREHIASHLQKLKLTLKRSNEVTQLKNEIPKGEWKKLKQDQCHLEAAKSTPELDQNVKNPVIQCDNNFHAPQQHSSKLANIFPDQSNVLNSIIQYDNNYHALQHSLTFTNNFTDQSSILNSVIQRVNNSHAPQHSPTFTNIFTDQSNMNPGDEFFN*