AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034200.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034200.01.T01 MTR_6g060600 79.060 702 55 5 1 653 1 659 0.0 1053
MsG0680034200.01.T01 MTR_6g460640 76.454 705 70 6 1 652 1 662 0.0 1034
MsG0680034200.01.T01 MTR_3g010790 45.694 569 214 14 83 644 1 481 3.70e-146 434
MsG0680034200.01.T01 MTR_3g078770 53.448 290 120 5 34 315 51 333 2.10e-108 341
MsG0680034200.01.T01 MTR_5g087790 52.189 297 134 3 29 318 43 338 2.07e-107 338
MsG0680034200.01.T01 MTR_2g098470 51.852 270 119 3 21 282 24 290 4.59e-93 300
MsG0680034200.01.T01 MTR_3g087530 48.837 258 110 6 35 279 32 280 1.34e-82 267
MsG0680034200.01.T01 MTR_8g091370 47.126 261 131 4 25 279 12 271 3.73e-76 251
MsG0680034200.01.T01 MTR_1g024420 75.238 105 18 1 29 125 4 108 1.01e-48 166
MsG0680034200.01.T01 MTR_6g060630 62.745 153 38 3 490 623 55 207 1.03e-48 169
MsG0680034200.01.T01 MTR_7g095580 45.000 60 33 0 77 136 6 65 3.48e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034200.01.T01 AT2G27050 55.805 267 107 3 29 286 43 307 2.53e-105 332
MsG0680034200.01.T01 AT3G20770 53.401 294 127 5 29 315 43 333 9.38e-105 331
MsG0680034200.01.T01 AT1G73730 49.451 273 125 3 18 282 21 288 5.48e-89 288
MsG0680034200.01.T01 AT1G73730 49.451 273 125 3 18 282 9 276 6.45e-89 288
MsG0680034200.01.T01 AT5G10120 49.615 260 123 3 28 279 16 275 6.64e-88 282
MsG0680034200.01.T01 AT5G65100 46.182 275 118 6 30 278 33 303 1.99e-78 260
MsG0680034200.01.T01 AT5G21120 38.147 367 199 10 40 387 60 417 1.05e-72 244
MsG0680034200.01.T01 AT5G21120 38.147 367 199 10 40 387 53 410 1.34e-72 243
MsG0680034200.01.T01 AT5G21120 38.147 367 199 10 40 387 53 410 1.34e-72 243

Find 122 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTTGATGCTGCATATTT+TGG 0.183879 6:+83319113 None:intergenic
CTTGATTGCCATGAAAATTT+TGG 0.189175 6:+83319089 None:intergenic
TAAATGATATGTTAGCTTAT+AGG 0.193817 6:-83320136 MsG0680034200.01.T01:CDS
AGCTTCGCCGCCGCTGATTA+TGG 0.223933 6:-83319484 MsG0680034200.01.T01:CDS
GCTAAATATGAAGCGGAAAA+TGG 0.224421 6:-83321073 MsG0680034200.01.T01:CDS
TGGCATCATCAAGTTTATAT+TGG 0.254374 6:-83318940 MsG0680034200.01.T01:CDS
TGCTTGTTTCAAACTTAAAA+AGG 0.257923 6:-83320103 MsG0680034200.01.T01:intron
ACAAGATAAACTCAGTATTA+AGG 0.259065 6:-83320699 MsG0680034200.01.T01:CDS
CGGAGATTTCCGTTGGAAAA+AGG 0.260283 6:-83320923 MsG0680034200.01.T01:CDS
AATGCTGCTAAGAAAGTTAA+AGG 0.267746 6:-83321925 MsG0680034200.01.T01:CDS
GTATTAAGGAAACATCTATT+TGG 0.288262 6:-83320685 MsG0680034200.01.T01:CDS
AGTAATTACCTGCATTGTTA+TGG 0.296901 6:+83319458 None:intergenic
AACAATGCAAAACAAGAAAA+AGG 0.304941 6:-83322002 MsG0680034200.01.T01:CDS
AGATTATGAGGCTTTCTATA+AGG 0.304992 6:+83320821 None:intergenic
TTCAGCGAGTGAAGTGTAAT+TGG 0.310216 6:+83321013 None:intergenic
CTTTGATTAACACCCTGATT+TGG 0.319131 6:+83319259 None:intergenic
GAACTACCCGACACCATATT+AGG 0.324492 6:-83320989 MsG0680034200.01.T01:CDS
GAGTACTATGATTCCAAATC+AGG 0.335331 6:-83319272 MsG0680034200.01.T01:CDS
TTGATGGACATATATAATTC+AGG 0.341441 6:-83319409 MsG0680034200.01.T01:CDS
AGAAGTGGTTTCTGATTTGA+TGG 0.347106 6:-83319425 MsG0680034200.01.T01:CDS
GGTTTGGAAGGTTTATGTTT+TGG 0.353709 6:-83320792 MsG0680034200.01.T01:CDS
GATGCTCGTGGCTTCATCTA+TGG 0.357885 6:-83321199 MsG0680034200.01.T01:CDS
TTCCTAATCTTATGAACATT+AGG 0.362413 6:+83320746 None:intergenic
GATTATGAGGCTTTCTATAA+GGG 0.363151 6:+83320822 None:intergenic
CAACTTCAACCATATCGAAT+TGG 0.368651 6:+83320285 None:intergenic
CAAACCTTCTTCAGATTATG+AGG 0.369249 6:+83320809 None:intergenic
GTGCACAAGAACACATATTA+AGG 0.370390 6:-83321839 MsG0680034200.01.T01:intron
CTCCATGATTCTTTCCCTGT+TGG 0.376931 6:+83320881 None:intergenic
CCTCCACCATGGTGGCCAAC+AGG 0.377036 6:-83320896 MsG0680034200.01.T01:CDS
CTCATAATCTGAAGAAGGTT+TGG 0.391254 6:-83320808 MsG0680034200.01.T01:CDS
ATCATCGTCATCGTCGTATG+AGG 0.393212 6:-83320477 MsG0680034200.01.T01:CDS
CCAAAATATGCAGCATCAAA+TGG 0.411899 6:-83319113 MsG0680034200.01.T01:CDS
GATAAGATCATTCATCATAT+AGG 0.419806 6:-83320194 MsG0680034200.01.T01:CDS
AGTTATGATTCTCTTGTTGA+AGG 0.423394 6:-83320602 MsG0680034200.01.T01:CDS
CACTTGCATGGTAGATTTGA+TGG 0.428886 6:+83319317 None:intergenic
TGAGAGAAGTGAACCTAATA+TGG 0.432462 6:+83320976 None:intergenic
AACATCCGATTGAGAATTGA+AGG 0.434143 6:+83318982 None:intergenic
CAGCAACAACCAATTCGATA+TGG 0.435824 6:-83320294 MsG0680034200.01.T01:CDS
CACTCATTGGTTTGCCCTCG+TGG 0.436143 6:+83321167 None:intergenic
CTACCATGCAAGTGTTCAAC+AGG 0.437195 6:-83319308 MsG0680034200.01.T01:CDS
GTTGCAACCATGTTCATAAC+TGG 0.438691 6:+83319148 None:intergenic
GTTGAGGCATTTGCTTGCAT+TGG 0.441840 6:+83319010 None:intergenic
TTGAAGTTTCCCCTCTTGAA+TGG 0.446304 6:+83322024 None:intergenic
ACATTAGCTACTTCAGCATT+AGG 0.464999 6:+83319034 None:intergenic
TACCGCAGCTTGCACACGTT+GGG 0.467304 6:+83320226 None:intergenic
AACAACCTTCAATTCTCAAT+CGG 0.467982 6:-83318987 MsG0680034200.01.T01:CDS
TTGTCTGATGAACCACTCAT+TGG 0.468990 6:+83321154 None:intergenic
CCTGTTGGCCACCATGGTGG+AGG 0.469951 6:+83320896 None:intergenic
TGAATGATCTTATCTGCAAC+AGG 0.473671 6:+83320203 None:intergenic
TTTCTTGGTGGAATTCATGT+TGG 0.474978 6:+83320363 None:intergenic
CTCCTAATGTTCATAAGATT+AGG 0.475251 6:-83320748 MsG0680034200.01.T01:CDS
CAGGTACATGTTAATGATGA+TGG 0.486249 6:-83321230 MsG0680034200.01.T01:CDS
AACGGAAATCTCCGTTGTGG+TGG 0.487895 6:+83320932 None:intergenic
ACAAATTATGAAAAGTTGAA+TGG 0.493728 6:-83321040 MsG0680034200.01.T01:CDS
CATCAGACAATTTACGAGGC+TGG 0.493913 6:-83321141 MsG0680034200.01.T01:CDS
GATAGCACCATTATGAACTC+TGG 0.501076 6:+83322052 None:intergenic
ATTCATCATATAGGTAATCA+TGG 0.501308 6:-83320185 MsG0680034200.01.T01:CDS
ACCATGTTCATAACTGGCAT+TGG 0.505692 6:+83319154 None:intergenic
AAGAGACAATATTATATCAC+TGG 0.506994 6:-83321889 MsG0680034200.01.T01:CDS
TGATGCTTACAACAACTTTG+TGG 0.507166 6:-83318960 MsG0680034200.01.T01:CDS
TCATATTTAGCAATTGTCGC+TGG 0.510330 6:+83321085 None:intergenic
GGCCAACAGGGAAAGAATCA+TGG 0.513222 6:-83320883 MsG0680034200.01.T01:CDS
AGTGAACCTAATATGGTGTC+GGG 0.518068 6:+83320983 None:intergenic
TCTTTCCCTGTTGGCCACCA+TGG 0.518755 6:+83320890 None:intergenic
ACTCATTGGTTTGCCCTCGT+GGG 0.520053 6:+83321168 None:intergenic
ATGTATCCCAGAGTTCATAA+TGG 0.523895 6:-83322059 MsG0680034200.01.T01:CDS
GCTTGCACACGTTGGGCAAG+AGG 0.525430 6:+83320233 None:intergenic
TTATGATTAACACCTGGAGT+TGG 0.531466 6:+83319346 None:intergenic
ATATTATATCACTGGAACAA+AGG 0.540439 6:-83321881 MsG0680034200.01.T01:CDS
TAATCTGAAGAAGGTTTGGA+AGG 0.542382 6:-83320804 MsG0680034200.01.T01:CDS
TCTCCTGTTGAACACTTGCA+TGG 0.543277 6:+83319305 None:intergenic
ATGAGATTTAGTGAAGATCC+TGG 0.545819 6:-83320851 MsG0680034200.01.T01:CDS
AACAAGAGAAAACATAAACT+TGG 0.546031 6:-83320449 MsG0680034200.01.T01:CDS
CATATCATTTACTAGATATG+TGG 0.546434 6:+83320148 None:intergenic
TATGGATTCCATAACAATGC+AGG 0.549065 6:-83319466 MsG0680034200.01.T01:CDS
ACCACAACGGAGATTTCCGT+TGG 0.549776 6:-83320930 MsG0680034200.01.T01:CDS
TTCCCTGTTGGCCACCATGG+TGG 0.549900 6:+83320893 None:intergenic
AAGTGAACCTAATATGGTGT+CGG 0.558954 6:+83320982 None:intergenic
AAAGCCTCATAATCTGAAGA+AGG 0.560995 6:-83320813 MsG0680034200.01.T01:CDS
GGAACATAGTCAGACAATCA+AGG 0.561355 6:-83320727 MsG0680034200.01.T01:CDS
CTCATTGGTTTGCCCTCGTG+GGG 0.563133 6:+83321169 None:intergenic
ACAAAGGAAAAGAAAGACAT+TGG 0.563170 6:-83321865 MsG0680034200.01.T01:CDS
CTCCACCATGGTGGCCAACA+GGG 0.563459 6:-83320895 MsG0680034200.01.T01:CDS
CATCATTGAAGTATTCATCA+TGG 0.563846 6:+83319183 None:intergenic
TGCCCAACGTGTGCAAGCTG+CGG 0.574797 6:-83320228 MsG0680034200.01.T01:CDS
AACAATGCAGGTAATTACTC+AGG 0.576216 6:-83319454 MsG0680034200.01.T01:CDS
GACAATTGCTAAATATGAAG+CGG 0.577001 6:-83321080 MsG0680034200.01.T01:CDS
AGAATTGAAGGTTGTTGTTG+AGG 0.577679 6:+83318994 None:intergenic
ATAGCACCATTATGAACTCT+GGG 0.587051 6:+83322053 None:intergenic
GCAGGTAATTACTCAGGAGG+AGG 0.589511 6:-83319448 MsG0680034200.01.T01:CDS
ATTATGAGGCTTTCTATAAG+GGG 0.590034 6:+83320823 None:intergenic
AGTACTATGATTCCAAATCA+GGG 0.594860 6:-83319271 MsG0680034200.01.T01:CDS
TCATCGTCATCGTCGTATGA+GGG 0.596584 6:-83320476 MsG0680034200.01.T01:CDS
AGTACGATGATTCCAACTCC+AGG 0.601419 6:-83319358 MsG0680034200.01.T01:CDS
GTTAACTATTGAGGAACTAG+AGG 0.610445 6:-83321967 MsG0680034200.01.T01:CDS
ACGGAAATCTCCGTTGTGGT+GGG 0.614439 6:+83320933 None:intergenic
TTACTCAGGAGGAGGAGAAG+TGG 0.624741 6:-83319440 MsG0680034200.01.T01:CDS
CAGACAATTTACGAGGCTGG+TGG 0.628174 6:-83321138 MsG0680034200.01.T01:CDS
TGGAATTCATGTTGGTCAAG+AGG 0.631768 6:+83320371 None:intergenic
TCCAACGGAAATCTCCGTTG+TGG 0.632183 6:+83320929 None:intergenic
CGTTGTGGTGGGTCACAATG+CGG 0.634330 6:+83320944 None:intergenic
CGCATTGTGACCCACCACAA+CGG 0.638363 6:-83320943 MsG0680034200.01.T01:CDS
GTACCGCAGCTTGCACACGT+TGG 0.638406 6:+83320225 None:intergenic
GCTATCGCTAACCATTCAAG+AGG 0.639976 6:-83322035 MsG0680034200.01.T01:CDS
TTATGAGGCTTTCTATAAGG+GGG 0.640626 6:+83320824 None:intergenic
TCCAATGCCAGTTATGAACA+TGG 0.642108 6:-83319155 MsG0680034200.01.T01:CDS
TGGAATCATCGTACTCATGA+CGG 0.645856 6:+83319366 None:intergenic
CTATCGCTAACCATTCAAGA+GGG 0.648395 6:-83322034 MsG0680034200.01.T01:CDS
GACGATGACGATGATGATGA+TGG 0.648657 6:+83320485 None:intergenic
ATGGAAGTTTGTGATGCTCG+TGG 0.649462 6:-83321211 MsG0680034200.01.T01:CDS
AAGTAAGTATGTGCTTGTTG+AGG 0.650722 6:-83320579 MsG0680034200.01.T01:CDS
AATGCAGGTAATTACTCAGG+AGG 0.654047 6:-83319451 MsG0680034200.01.T01:CDS
CACGAGGGCAAACCAATGAG+TGG 0.675933 6:-83321166 MsG0680034200.01.T01:CDS
ATGGAATCCATAATCAGCGG+CGG 0.694876 6:+83319477 None:intergenic
CTATGGAGTAATACCCCACG+AGG 0.701029 6:-83321182 MsG0680034200.01.T01:CDS
GTTATGGAATCCATAATCAG+CGG 0.702738 6:+83319474 None:intergenic
TGCATGTTATGATTAACACC+TGG 0.705952 6:+83319340 None:intergenic
AAGGCATTCCTCCACCATGG+TGG 0.706316 6:-83320904 MsG0680034200.01.T01:CDS
TATCGCTAACCATTCAAGAG+GGG 0.726590 6:-83322033 MsG0680034200.01.T01:CDS
GGTTCATCAGACAATTTACG+AGG 0.727611 6:-83321145 MsG0680034200.01.T01:CDS
CAACAGGGAAAGAATCATGG+AGG 0.743204 6:-83320880 MsG0680034200.01.T01:CDS
TATGGAGTAATACCCCACGA+GGG 0.783328 6:-83321181 MsG0680034200.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTTCAACCTAATTATT+TGG + Chr6:83319556-83319575 None:intergenic 15.0%
!! CTATTTCATTATATATTACT+TGG - Chr6:83321190-83321209 MsG0680034200.01.T01:CDS 15.0%
!! TATAATTCACAATTTGATTA+TGG - Chr6:83321076-83321095 MsG0680034200.01.T01:CDS 15.0%
!! TCCTAGTTAAAATAAAAATA+AGG + Chr6:83319183-83319202 None:intergenic 15.0%
!!! ACCTTATTTTTATTTTAACT+AGG - Chr6:83319179-83319198 MsG0680034200.01.T01:CDS 15.0%
!! AATTCAAATCTCATTTAAAG+CGG + Chr6:83319285-83319304 None:intergenic 20.0%
!! ACAAATTATGAAAAGTTGAA+TGG - Chr6:83319950-83319969 MsG0680034200.01.T01:intron 20.0%
!! ATAGTTAAATTCGACAAAAA+CGG - Chr6:83319881-83319900 MsG0680034200.01.T01:intron 20.0%
!! ATGTAAGTATATTTAATACG+AGG - Chr6:83319601-83319620 MsG0680034200.01.T01:intron 20.0%
!! CTTAAAAAGGTTATTTAACT+AGG - Chr6:83320900-83320919 MsG0680034200.01.T01:CDS 20.0%
!! TTAAACCAACAAAATTGTTT+GGG + Chr6:83321262-83321281 None:intergenic 20.0%
!! TTTAAACCAACAAAATTGTT+TGG + Chr6:83321263-83321282 None:intergenic 20.0%
!!! AACTTGAATTTTAGTTTACT+TGG - Chr6:83319406-83319425 MsG0680034200.01.T01:CDS 20.0%
!!! AATTTTGTTGGTTTAAATAG+AGG - Chr6:83321266-83321285 MsG0680034200.01.T01:intron 20.0%
!!! GTATTTTTTGCCAAATAATT+AGG - Chr6:83319543-83319562 MsG0680034200.01.T01:intron 20.0%
!!! TAAATGATATGTTAGCTTAT+AGG - Chr6:83320854-83320873 MsG0680034200.01.T01:CDS 20.0%
!!! TTATTCATTTTCGATTTACT+TGG - Chr6:83319379-83319398 MsG0680034200.01.T01:CDS 20.0%
! AACAAGAGAAAACATAAACT+TGG - Chr6:83320541-83320560 MsG0680034200.01.T01:CDS 25.0%
! AACAATGCAAAACAAGAAAA+AGG - Chr6:83318988-83319007 MsG0680034200.01.T01:CDS 25.0%
! AAGAGACAATATTATATCAC+TGG - Chr6:83319101-83319120 MsG0680034200.01.T01:CDS 25.0%
! ACAAGATAAACTCAGTATTA+AGG - Chr6:83320291-83320310 MsG0680034200.01.T01:CDS 25.0%
! ATATTATATCACTGGAACAA+AGG - Chr6:83319109-83319128 MsG0680034200.01.T01:CDS 25.0%
! ATTCATCATATAGGTAATCA+TGG - Chr6:83320805-83320824 MsG0680034200.01.T01:CDS 25.0%
! CATATCATTTACTAGATATG+TGG + Chr6:83320845-83320864 None:intergenic 25.0%
! GATAAGATCATTCATCATAT+AGG - Chr6:83320796-83320815 MsG0680034200.01.T01:CDS 25.0%
! GTATTAAGGAAACATCTATT+TGG - Chr6:83320305-83320324 MsG0680034200.01.T01:CDS 25.0%
! TAAAATCACAAGTTAACTCA+TGG - Chr6:83319326-83319345 MsG0680034200.01.T01:CDS 25.0%
! TAAACCAACAAAATTGTTTG+GGG + Chr6:83321261-83321280 None:intergenic 25.0%
! TCATAACTTGAGAAAAATTC+AGG + Chr6:83320376-83320395 None:intergenic 25.0%
! TCATACGCTTTCATAAATTT+AGG - Chr6:83319351-83319370 MsG0680034200.01.T01:CDS 25.0%
! TGCTTGTTTCAAACTTAAAA+AGG - Chr6:83320887-83320906 MsG0680034200.01.T01:CDS 25.0%
! TTCCTAATCTTATGAACATT+AGG + Chr6:83320247-83320266 None:intergenic 25.0%
! TTTGTCTATAAAGAATCAAC+TGG + Chr6:83319658-83319677 None:intergenic 25.0%
!! AAAATTTTCATGGCAATCAA+GGG - Chr6:83321903-83321922 MsG0680034200.01.T01:CDS 25.0%
!! TAGCTTATAGGTATCAATTT+TGG - Chr6:83320930-83320949 MsG0680034200.01.T01:CDS 25.0%
!! TTGATGGACATATATAATTC+AGG - Chr6:83321581-83321600 MsG0680034200.01.T01:intron 25.0%
!!! AAGCTAACATTATTTTTACG+AGG + Chr6:83321491-83321510 None:intergenic 25.0%
!!! AGCTAACATTATTTTTACGA+GGG + Chr6:83321490-83321509 None:intergenic 25.0%
!!! CCAGATTTATTTTTCTTTTG+GGG + Chr6:83321144-83321163 None:intergenic 25.0%
!!! GCCAGATTTATTTTTCTTTT+GGG + Chr6:83321145-83321164 None:intergenic 25.0%
!!! TGCATATTTTGGTTAAATCT+GGG + Chr6:83321869-83321888 None:intergenic 25.0%
!!! TGCCAGATTTATTTTTCTTT+TGG + Chr6:83321146-83321165 None:intergenic 25.0%
AACAACCTTCAATTCTCAAT+CGG - Chr6:83322003-83322022 MsG0680034200.01.T01:CDS 30.0%
AATGCTGCTAAGAAAGTTAA+AGG - Chr6:83319065-83319084 MsG0680034200.01.T01:CDS 30.0%
ACAAAGGAAAAGAAAGACAT+TGG - Chr6:83319125-83319144 MsG0680034200.01.T01:CDS 30.0%
AGATTATGAGGCTTTCTATA+AGG + Chr6:83320172-83320191 None:intergenic 30.0%
AGTAATTACCTGCATTGTTA+TGG + Chr6:83321535-83321554 None:intergenic 30.0%
AGTACTATGATTCCAAATCA+GGG - Chr6:83321719-83321738 MsG0680034200.01.T01:intron 30.0%
AGTTATGATTCTCTTGTTGA+AGG - Chr6:83320388-83320407 MsG0680034200.01.T01:CDS 30.0%
ATTATGAGGCTTTCTATAAG+GGG + Chr6:83320170-83320189 None:intergenic 30.0%
CCCCAAAAGAAAAATAAATC+TGG - Chr6:83321141-83321160 MsG0680034200.01.T01:CDS 30.0%
CTCCTAATGTTCATAAGATT+AGG - Chr6:83320242-83320261 MsG0680034200.01.T01:CDS 30.0%
GACAATTGCTAAATATGAAG+CGG - Chr6:83319910-83319929 MsG0680034200.01.T01:intron 30.0%
GATTATGAGGCTTTCTATAA+GGG + Chr6:83320171-83320190 None:intergenic 30.0%
GATTTGAATTCTATCTCTTG+AGG - Chr6:83319295-83319314 MsG0680034200.01.T01:CDS 30.0%
GGAAAAAGAGTTAACTATTG+AGG - Chr6:83319014-83319033 MsG0680034200.01.T01:CDS 30.0%
TCACAATTTGATTATGGTCA+GGG - Chr6:83321082-83321101 MsG0680034200.01.T01:CDS 30.0%
TGCAAAACAAGAAAAAGGAA+AGG - Chr6:83318993-83319012 MsG0680034200.01.T01:CDS 30.0%
TGGCATCATCAAGTTTATAT+TGG - Chr6:83322050-83322069 MsG0680034200.01.T01:CDS 30.0%
TTCACAATTTGATTATGGTC+AGG - Chr6:83321081-83321100 MsG0680034200.01.T01:CDS 30.0%
! AAATTTTCATGGCAATCAAG+GGG - Chr6:83321904-83321923 MsG0680034200.01.T01:CDS 30.0%
! CAAAATTTTCATGGCAATCA+AGG - Chr6:83321902-83321921 MsG0680034200.01.T01:CDS 30.0%
! CAAATGGACCAAAATTTTCA+TGG - Chr6:83321893-83321912 MsG0680034200.01.T01:CDS 30.0%
! CATCATTGAAGTATTCATCA+TGG + Chr6:83321810-83321829 None:intergenic 30.0%
! CTAGGAAAATGTTAGCTTAT+AGG - Chr6:83320918-83320937 MsG0680034200.01.T01:CDS 30.0%
! CTTGATTGCCATGAAAATTT+TGG + Chr6:83321904-83321923 None:intergenic 30.0%
!! AATGATTTTGATGTTGTGGA+AGG - Chr6:83320436-83320455 MsG0680034200.01.T01:CDS 30.0%
!! CTTGTTGTTAATCATAGCAT+TGG - Chr6:83321232-83321251 MsG0680034200.01.T01:intron 30.0%
!! TGACTCATGCTTTAGAAAAT+AGG - Chr6:83321050-83321069 MsG0680034200.01.T01:CDS 30.0%
!!! CTAACATTATTTTTACGAGG+GGG + Chr6:83321488-83321507 None:intergenic 30.0%
!!! CTGCATATTTTGGTTAAATC+TGG + Chr6:83321870-83321889 None:intergenic 30.0%
!!! GCTAACATTATTTTTACGAG+GGG + Chr6:83321489-83321508 None:intergenic 30.0%
!!! TGTTCCTCACATTTTTTTCT+TGG + Chr6:83320645-83320664 None:intergenic 30.0%
AAAGCCTCATAATCTGAAGA+AGG - Chr6:83320177-83320196 MsG0680034200.01.T01:CDS 35.0%
AACAATGCAGGTAATTACTC+AGG - Chr6:83321536-83321555 MsG0680034200.01.T01:intron 35.0%
AACATCCGATTGAGAATTGA+AGG + Chr6:83322011-83322030 None:intergenic 35.0%
AAGGCAAAGTTCTCAAAATC+AGG + Chr6:83319698-83319717 None:intergenic 35.0%
AAGTGAACCTAATATGGTGT+CGG + Chr6:83320011-83320030 None:intergenic 35.0%
ACATTAGCTACTTCAGCATT+AGG + Chr6:83321959-83321978 None:intergenic 35.0%
ATAGCACCATTATGAACTCT+GGG + Chr6:83318940-83318959 None:intergenic 35.0%
ATGAGATTTAGTGAAGATCC+TGG - Chr6:83320139-83320158 MsG0680034200.01.T01:CDS 35.0%
ATGTATCCCAGAGTTCATAA+TGG - Chr6:83318931-83318950 MsG0680034200.01.T01:CDS 35.0%
CAAACCTTCTTCAGATTATG+AGG + Chr6:83320184-83320203 None:intergenic 35.0%
CAACTTCAACCATATCGAAT+TGG + Chr6:83320708-83320727 None:intergenic 35.0%
CACAATTTGATTATGGTCAG+GGG - Chr6:83321083-83321102 MsG0680034200.01.T01:CDS 35.0%
CAGGTACATGTTAATGATGA+TGG - Chr6:83319760-83319779 MsG0680034200.01.T01:intron 35.0%
CCAAAATATGCAGCATCAAA+TGG - Chr6:83321877-83321896 MsG0680034200.01.T01:CDS 35.0%
CTCATAATCTGAAGAAGGTT+TGG - Chr6:83320182-83320201 MsG0680034200.01.T01:CDS 35.0%
CTTTGATTAACACCCTGATT+TGG + Chr6:83321734-83321753 None:intergenic 35.0%
GAGTACTATGATTCCAAATC+AGG - Chr6:83321718-83321737 MsG0680034200.01.T01:intron 35.0%
GCTAAATATGAAGCGGAAAA+TGG - Chr6:83319917-83319936 MsG0680034200.01.T01:intron 35.0%
GTGCACAAGAACACATATTA+AGG - Chr6:83319151-83319170 MsG0680034200.01.T01:CDS 35.0%
GTTAACTATTGAGGAACTAG+AGG - Chr6:83319023-83319042 MsG0680034200.01.T01:CDS 35.0%
GTTATGGAATCCATAATCAG+CGG + Chr6:83321519-83321538 None:intergenic 35.0%
TAATCTGAAGAAGGTTTGGA+AGG - Chr6:83320186-83320205 MsG0680034200.01.T01:CDS 35.0%
TATGGATTCCATAACAATGC+AGG - Chr6:83321524-83321543 MsG0680034200.01.T01:intron 35.0%
TCATATTTAGCAATTGTCGC+TGG + Chr6:83319908-83319927 None:intergenic 35.0%
TCCACCAAGAAAAAAATGTG+AGG - Chr6:83320638-83320657 MsG0680034200.01.T01:CDS 35.0%
TCTGAGCTTATAGTGAACTA+AGG - Chr6:83319449-83319468 MsG0680034200.01.T01:CDS 35.0%
TGAATGATCTTATCTGCAAC+AGG + Chr6:83320790-83320809 None:intergenic 35.0%
TGAGAGAAGTGAACCTAATA+TGG + Chr6:83320017-83320036 None:intergenic 35.0%
TGATGCTTACAACAACTTTG+TGG - Chr6:83322030-83322049 MsG0680034200.01.T01:CDS 35.0%
TGCATGTTATGATTAACACC+TGG + Chr6:83321653-83321672 None:intergenic 35.0%
TTATGAGGCTTTCTATAAGG+GGG + Chr6:83320169-83320188 None:intergenic 35.0%
TTATGATTAACACCTGGAGT+TGG + Chr6:83321647-83321666 None:intergenic 35.0%
TTTCTTGGTGGAATTCATGT+TGG + Chr6:83320630-83320649 None:intergenic 35.0%
! AAGTAAGTATGTGCTTGTTG+AGG - Chr6:83320411-83320430 MsG0680034200.01.T01:CDS 35.0%
! AATTGACTTTGTTCACCTCA+AGG + Chr6:83319717-83319736 None:intergenic 35.0%
! AATTTTCATGGCAATCAAGG+GGG - Chr6:83321905-83321924 MsG0680034200.01.T01:CDS 35.0%
! AGAAGTGGTTTCTGATTTGA+TGG - Chr6:83321565-83321584 MsG0680034200.01.T01:intron 35.0%
! AGAATTGAAGGTTGTTGTTG+AGG + Chr6:83321999-83322018 None:intergenic 35.0%
! CCATTTGATGCTGCATATTT+TGG + Chr6:83321880-83321899 None:intergenic 35.0%
! GGTTTGGAAGGTTTATGTTT+TGG - Chr6:83320198-83320217 MsG0680034200.01.T01:CDS 35.0%
!! ATTTTGAGAACTTTGCCTTG+AGG - Chr6:83319699-83319718 MsG0680034200.01.T01:intron 35.0%
!! CATTTTACTAGCGCTGAAAT+AGG - Chr6:83320670-83320689 MsG0680034200.01.T01:CDS 35.0%
!! GGCAAATGATTTTGATGTTG+TGG - Chr6:83320432-83320451 MsG0680034200.01.T01:CDS 35.0%
!!! TCCTCACATTTTTTTCTTGG+TGG + Chr6:83320642-83320661 None:intergenic 35.0%
AACATGTACCTGTTCACACT+AGG + Chr6:83319752-83319771 None:intergenic 40.0%
AATGCAGGTAATTACTCAGG+AGG - Chr6:83321539-83321558 MsG0680034200.01.T01:intron 40.0%
ACCATGTTCATAACTGGCAT+TGG + Chr6:83321839-83321858 None:intergenic 40.0%
AGTGAACCTAATATGGTGTC+GGG + Chr6:83320010-83320029 None:intergenic 40.0%
ATTCTATCTCTTGAGGCAAG+TGG - Chr6:83319302-83319321 MsG0680034200.01.T01:CDS 40.0%
CACTTGCATGGTAGATTTGA+TGG + Chr6:83321676-83321695 None:intergenic 40.0%
CAGCAACAACCAATTCGATA+TGG - Chr6:83320696-83320715 MsG0680034200.01.T01:CDS 40.0%
CTATCGCTAACCATTCAAGA+GGG - Chr6:83318956-83318975 MsG0680034200.01.T01:CDS 40.0%
GATAGCACCATTATGAACTC+TGG + Chr6:83318941-83318960 None:intergenic 40.0%
GGAACATAGTCAGACAATCA+AGG - Chr6:83320263-83320282 MsG0680034200.01.T01:CDS 40.0%
GGTTCATCAGACAATTTACG+AGG - Chr6:83319845-83319864 MsG0680034200.01.T01:intron 40.0%
GTTGCAACCATGTTCATAAC+TGG + Chr6:83321845-83321864 None:intergenic 40.0%
TATCGCTAACCATTCAAGAG+GGG - Chr6:83318957-83318976 MsG0680034200.01.T01:CDS 40.0%
TCCAATGCCAGTTATGAACA+TGG - Chr6:83321835-83321854 MsG0680034200.01.T01:intron 40.0%
TGGAATCATCGTACTCATGA+CGG + Chr6:83321627-83321646 None:intergenic 40.0%
TGGAATTCATGTTGGTCAAG+AGG + Chr6:83320622-83320641 None:intergenic 40.0%
TTCAGCGAGTGAAGTGTAAT+TGG + Chr6:83319980-83319999 None:intergenic 40.0%
TTGAAGTTTCCCCTCTTGAA+TGG + Chr6:83318969-83318988 None:intergenic 40.0%
TTGTCTGATGAACCACTCAT+TGG + Chr6:83319839-83319858 None:intergenic 40.0%
! GCTACCCCAAACAATTTTGT+TGG - Chr6:83321254-83321273 MsG0680034200.01.T01:intron 40.0%
!! GATTTTGATGTTGTGGAAGG+TGG - Chr6:83320439-83320458 MsG0680034200.01.T01:CDS 40.0%
!!! GAAGGTTTATGTTTTGGCAG+CGG - Chr6:83320204-83320223 MsG0680034200.01.T01:CDS 40.0%
AGTACGATGATTCCAACTCC+AGG - Chr6:83321632-83321651 MsG0680034200.01.T01:intron 45.0%
ATCATCGTCATCGTCGTATG+AGG - Chr6:83320513-83320532 MsG0680034200.01.T01:CDS 45.0%
ATGCTACCCCATTGATGTGA+AGG + Chr6:83321115-83321134 None:intergenic 45.0%
ATGGAAGTTTGTGATGCTCG+TGG - Chr6:83319779-83319798 MsG0680034200.01.T01:intron 45.0%
ATGGAATCCATAATCAGCGG+CGG + Chr6:83321516-83321535 None:intergenic 45.0%
CAACAGGGAAAGAATCATGG+AGG - Chr6:83320110-83320129 MsG0680034200.01.T01:CDS 45.0%
CATCAGACAATTTACGAGGC+TGG - Chr6:83319849-83319868 MsG0680034200.01.T01:intron 45.0%
CGAATCCCTTCACATCAATG+GGG - Chr6:83321106-83321125 MsG0680034200.01.T01:CDS 45.0%
CGGAGATTTCCGTTGGAAAA+AGG - Chr6:83320067-83320086 MsG0680034200.01.T01:intron 45.0%
CTACCATGCAAGTGTTCAAC+AGG - Chr6:83321682-83321701 MsG0680034200.01.T01:intron 45.0%
CTCCATGATTCTTTCCCTGT+TGG + Chr6:83320112-83320131 None:intergenic 45.0%
GAACTACCCGACACCATATT+AGG - Chr6:83320001-83320020 MsG0680034200.01.T01:intron 45.0%
GCGAATCCCTTCACATCAAT+GGG - Chr6:83321105-83321124 MsG0680034200.01.T01:CDS 45.0%
GCTATCGCTAACCATTCAAG+AGG - Chr6:83318955-83318974 MsG0680034200.01.T01:CDS 45.0%
TATGGAGTAATACCCCACGA+GGG - Chr6:83319809-83319828 MsG0680034200.01.T01:intron 45.0%
TCACATCAATGGGGTAGCAT+TGG - Chr6:83321115-83321134 MsG0680034200.01.T01:CDS 45.0%
TCATCGTCATCGTCGTATGA+GGG - Chr6:83320514-83320533 MsG0680034200.01.T01:CDS 45.0%
TCTCCTGTTGAACACTTGCA+TGG + Chr6:83321688-83321707 None:intergenic 45.0%
TGCTACCCCATTGATGTGAA+GGG + Chr6:83321114-83321133 None:intergenic 45.0%
! GACGATGACGATGATGATGA+TGG + Chr6:83320508-83320527 None:intergenic 45.0%
! GGAGGAATGCCTTTTTCCAA+CGG + Chr6:83320079-83320098 None:intergenic 45.0%
! GTTGAGGCATTTGCTTGCAT+TGG + Chr6:83321983-83322002 None:intergenic 45.0%
!! AAAAAGGCATTCCTCCACCA+TGG - Chr6:83320083-83320102 MsG0680034200.01.T01:intron 45.0%
AACGGAAATCTCCGTTGTGG+TGG + Chr6:83320061-83320080 None:intergenic 50.0%
ACCACAACGGAGATTTCCGT+TGG - Chr6:83320060-83320079 MsG0680034200.01.T01:intron 50.0%
ACGGAAATCTCCGTTGTGGT+GGG + Chr6:83320060-83320079 None:intergenic 50.0%
AGCTGACACCTAGTGTGAAC+AGG - Chr6:83319741-83319760 MsG0680034200.01.T01:intron 50.0%
CAGACAATTTACGAGGCTGG+TGG - Chr6:83319852-83319871 MsG0680034200.01.T01:intron 50.0%
CTATGGAGTAATACCCCACG+AGG - Chr6:83319808-83319827 MsG0680034200.01.T01:intron 50.0%
GAGGCTTTCTATAAGGGGGT+AGG + Chr6:83320165-83320184 None:intergenic 50.0%
GATGCTCGTGGCTTCATCTA+TGG - Chr6:83319791-83319810 MsG0680034200.01.T01:intron 50.0%
GCAGGTAATTACTCAGGAGG+AGG - Chr6:83321542-83321561 MsG0680034200.01.T01:intron 50.0%
GGCCAACAGGGAAAGAATCA+TGG - Chr6:83320107-83320126 MsG0680034200.01.T01:CDS 50.0%
GGCGAATCCCTTCACATCAA+TGG - Chr6:83321104-83321123 MsG0680034200.01.T01:CDS 50.0%
TCCAACGGAAATCTCCGTTG+TGG + Chr6:83320064-83320083 None:intergenic 50.0%
TTACTCAGGAGGAGGAGAAG+TGG - Chr6:83321550-83321569 MsG0680034200.01.T01:intron 50.0%
TTTCTATAAGGGGGTAGGCC+AGG + Chr6:83320160-83320179 None:intergenic 50.0%
!! ACTCATTGGTTTGCCCTCGT+GGG + Chr6:83319825-83319844 None:intergenic 50.0%
CACGAGGGCAAACCAATGAG+TGG - Chr6:83319824-83319843 MsG0680034200.01.T01:intron 55.0%
CGCATTGTGACCCACCACAA+CGG - Chr6:83320047-83320066 MsG0680034200.01.T01:intron 55.0%
TACCGCAGCTTGCACACGTT+GGG + Chr6:83320767-83320786 None:intergenic 55.0%
TCTTTCCCTGTTGGCCACCA+TGG + Chr6:83320103-83320122 None:intergenic 55.0%
! AAGGCATTCCTCCACCATGG+TGG - Chr6:83320086-83320105 MsG0680034200.01.T01:intron 55.0%
!! CACTCATTGGTTTGCCCTCG+TGG + Chr6:83319826-83319845 None:intergenic 55.0%
!! CGTTGTGGTGGGTCACAATG+CGG + Chr6:83320049-83320068 None:intergenic 55.0%
!! CTCATTGGTTTGCCCTCGTG+GGG + Chr6:83319824-83319843 None:intergenic 55.0%
CTCCACCATGGTGGCCAACA+GGG - Chr6:83320095-83320114 MsG0680034200.01.T01:intron 60.0%
GCTTGCACACGTTGGGCAAG+AGG + Chr6:83320760-83320779 None:intergenic 60.0%
GTACCGCAGCTTGCACACGT+TGG + Chr6:83320768-83320787 None:intergenic 60.0%
TGCCCAACGTGTGCAAGCTG+CGG - Chr6:83320762-83320781 MsG0680034200.01.T01:CDS 60.0%
TTCCCTGTTGGCCACCATGG+TGG + Chr6:83320100-83320119 None:intergenic 60.0%
! AGCTTCGCCGCCGCTGATTA+TGG - Chr6:83321506-83321525 MsG0680034200.01.T01:intron 60.0%
CCTCCACCATGGTGGCCAAC+AGG - Chr6:83320094-83320113 MsG0680034200.01.T01:intron 65.0%
CCTGTTGGCCACCATGGTGG+AGG + Chr6:83320097-83320116 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 83318931 83322081 83318931 ID=MsG0680034200.01;Name=MsG0680034200.01
Chr6 mRNA 83318931 83322081 83318931 ID=MsG0680034200.01.T01;Parent=MsG0680034200.01;Name=MsG0680034200.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|0.5|0.66|3|0|653
Chr6 exon 83321840 83322081 83321840 ID=MsG0680034200.01.T01:exon:11728;Parent=MsG0680034200.01.T01
Chr6 exon 83320104 83321249 83320104 ID=MsG0680034200.01.T01:exon:11729;Parent=MsG0680034200.01.T01
Chr6 exon 83318931 83319504 83318931 ID=MsG0680034200.01.T01:exon:11730;Parent=MsG0680034200.01.T01
Chr6 CDS 83321840 83322081 83321840 ID=MsG0680034200.01.T01:cds;Parent=MsG0680034200.01.T01
Chr6 CDS 83320104 83321249 83320104 ID=MsG0680034200.01.T01:cds;Parent=MsG0680034200.01.T01
Chr6 CDS 83318931 83319504 83318931 ID=MsG0680034200.01.T01:cds;Parent=MsG0680034200.01.T01
Gene Sequence

>MsG0680034200.01.T01

ATGTATCCCAGAGTTCATAATGGTGCTATCGCTAACCATTCAAGAGGGGAAACTTCAAACAATGCAAAACAAGAAAAAGGAAAGGAAAAAGAGTTAACTATTGAGGAACTAGAGGCAAAGATATATAAAGATGAAATGCTGCTAAGAAAGTTAAAGGAAGAAAGAAGTAAAAGAGACAATATTATATCACTGGAACAAAGGAAAAGAAAGACATTGGCTCGTGCACAAGAACACATATTAAGGTACATGTTAATGATGATGGAAGTTTGTGATGCTCGTGGCTTCATCTATGGAGTAATACCCCACGAGGGCAAACCAATGAGTGGTTCATCAGACAATTTACGAGGCTGGTGGAAAGATATAGTTAAATTCGACAAAAACGGTCCAGCGACAATTGCTAAATATGAAGCGGAAAATGGAATTACAACAACAAATTATGAAAAGTTGAATGGTGAACCAATTACACTTCACTCGCTGAATGAACTACCCGACACCATATTAGGTTCACTTCTCTCATCATTAGTGCCGCATTGTGACCCACCACAACGGAGATTTCCGTTGGAAAAAGGCATTCCTCCACCATGGTGGCCAACAGGGAAAGAATCATGGAGGAATGAGATGAGATTTAGTGAAGATCCTGGCCTACCCCCTTATAGAAAGCCTCATAATCTGAAGAAGGTTTGGAAGGTTTATGTTTTGGCAGCGGTTATTAAACATATGTCTCCTAATGTTCATAAGATTAGGAACATAGTCAGACAATCAAGGAGTTTACAAGATAAACTCAGTATTAAGGAAACATCTATTTGGTTTGCTATTATTAATCATGAAGAAACAATTGCAAGAAAAATACATCCTGAATTTTTCTCAAGTTATGATTCTCTTGTTGAAGGAAGTAAGTATGTGCTTGTTGAGGCAAATGATTTTGATGTTGTGGAAGGTGGTGAGCACAATCTAGCAAAACGAAAACTATCATCATCATCTTCACCATCATCATCATCGTCATCGTCGTATGAGGGTACTAACAAGAGAAAACATAAACTTGGCAAGAGAATTAGTACTCATCATAACTCTTTTCTTAACACTCATCAACATGCTACACCTCTTGACCAACATGAATTCCACCAAGAAAAAAATGTGAGGAACAATCATCATTTTACTAGCGCTGAAATAGGAAGCAGCAACAACCAATTCGATATGGTTGAAGTTGAAGTGTCAACAACTCATCAAAATGTTGCACCTCTTGCCCAACGTGTGCAAGCTGCGGTACCTGTTGCAGATAAGATCATTCATCATATAGGTAATCATGGTTATCATTATTCAGCCACATATCTAGTAAATGATATGTTAGCTTATAGGTTTGCGAAGTTGCTTGTTTCAAACTTAAAAAGCTTCGCCGCCGCTGATTATGGATTCCATAACAATGCAGGTAATTACTCAGGAGGAGGAGAAGTGGTTTCTGATTTGATGGACATATATAATTCAGGCATTCAGCTGAATAAAAACACCGTCATGAGTACGATGATTCCAACTCCAGGTGTTAATCATAACATGCACCATCAAATCTACCATGCAAGTGTTCAACAGGAGAAGAACAACATGAGTACTATGATTCCAAATCAGGGTGTTAATCAAAGCATGCAACGTCAAATCTACACTTCAAGTGTTCATCAACAACAGAAGAGCAACACCATGATGAATACTTCAATGATGAGTACTCCAATGCCAGTTATGAACATGGTTGCAACTCCCAGATTTAACCAAAATATGCAGCATCAAATGGACCAAAATTTTCATGGCAATCAAGGGGGTGCAAATAGCTACTATCACTGCAAAGTGCCTAATGCTGAAGTAGCTAATGTTCCAATGCAAGCAAATGCCTCAACAACAACCTTCAATTCTCAATCGGATGTTGATGCTTACAACAACTTTGTGGCATCATCAAGTTTATATTGGAATCAATAG
Protein sequence

>MsG0680034200.01.T01

MYPRVHNGAIANHSRGETSNNAKQEKGKEKELTIEELEAKIYKDEMLLRKLKEERSKRDNIISLEQRKRKTLARAQEHILRYMLMMMEVCDARGFIYGVIPHEGKPMSGSSDNLRGWWKDIVKFDKNGPATIAKYEAENGITTTNYEKLNGEPITLHSLNELPDTILGSLLSSLVPHCDPPQRRFPLEKGIPPPWWPTGKESWRNEMRFSEDPGLPPYRKPHNLKKVWKVYVLAAVIKHMSPNVHKIRNIVRQSRSLQDKLSIKETSIWFAIINHEETIARKIHPEFFSSYDSLVEGSKYVLVEANDFDVVEGGEHNLAKRKLSSSSSPSSSSSSSYEGTNKRKHKLGKRISTHHNSFLNTHQHATPLDQHEFHQEKNVRNNHHFTSAEIGSSNNQFDMVEVEVSTTHQNVAPLAQRVQAAVPVADKIIHHIGNHGYHYSATYLVNDMLAYRFAKLLVSNLKSFAAADYGFHNNAGNYSGGGEVVSDLMDIYNSGIQLNKNTVMSTMIPTPGVNHNMHHQIYHASVQQEKNNMSTMIPNQGVNQSMQRQIYTSSVHQQQKSNTMMNTSMMSTPMPVMNMVATPRFNQNMQHQMDQNFHGNQGGANSYYHCKVPNAEVANVPMQANASTTTFNSQSDVDAYNNFVASSSLYWNQ*