AlfalfaGEDB | MsG0680034895.01

M. sativa cultivar ZhongmuNo.1 / MsG0680034895.01

KEGG: -
GO: GO:0003677 GO:0006355
PFAM: PF02365

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034895.01.T01	QSD99826.1	82.283	254	29	3	113	350	1	254	8.75E-140	407

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034895.01.T01	Q9FY93	71.257	167	46	1	113	277	1	167	3.60E-85	261

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034895.01.T01	A0A896WEL3	82.283	254	29	3	113	350	1	254	4.18e-140	407

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TFs/TRs:

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Gene ID	Type	Classification
MsG0680034895.01.T01	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034895.01.T01	MTR_7g005280	82.031	256	25	7	113	350	1	253	3.36e-134	383
MsG0680034895.01.T01	MTR_8g099750	61.988	171	56	3	113	274	1	171	3.73e-69	216
MsG0680034895.01.T01	MTR_3g098810	57.627	177	63	3	113	277	1	177	5.80e-64	203
MsG0680034895.01.T01	MTR_3g098810	56.287	167	66	3	113	272	1	167	1.18e-60	192
MsG0680034895.01.T01	MTR_4g089135	53.297	182	56	3	126	278	15	196	3.62e-55	184
MsG0680034895.01.T01	MTR_7g070140	57.222	180	17	3	171	350	12	131	3.04e-54	175
MsG0680034895.01.T01	MTR_2g080010	52.660	188	64	5	126	288	15	202	4.82e-54	181
MsG0680034895.01.T01	MTR_7g085220	45.854	205	84	5	123	309	5	200	1.42e-53	180
MsG0680034895.01.T01	MTR_4g081870	56.604	159	57	4	126	274	9	165	2.03e-52	174
MsG0680034895.01.T01	MTR_5g041940	55.696	158	58	3	124	271	1	156	2.33e-52	174

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034895.01.T01	AT5G13180	71.257	167	46	1	113	277	1	167	3.67e-86	261
MsG0680034895.01.T01	AT2G33480	70.909	165	44	3	113	275	1	163	2.70e-80	246
MsG0680034895.01.T01	AT2G33480	70.060	167	44	3	113	275	1	165	4.69e-80	246
MsG0680034895.01.T01	AT1G61110	55.056	178	59	5	113	270	1	177	1.09e-53	179
MsG0680034895.01.T01	AT1G65910	43.913	230	113	5	124	345	4	225	7.28e-53	184
MsG0680034895.01.T01	AT3G04070	55.090	167	59	4	120	270	4	170	8.17e-53	178
MsG0680034895.01.T01	AT1G69490	55.152	165	62	3	126	280	9	171	8.50e-53	176
MsG0680034895.01.T01	AT1G77450	46.226	212	100	4	121	322	5	212	3.75e-52	173
MsG0680034895.01.T01	AT3G04070	55.556	162	58	3	120	275	4	157	1.50e-51	175
MsG0680034895.01.T01	AT3G15510	56.329	158	52	3	130	270	21	178	2.06e-50	172

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Find 80 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGCCAAGTATCCAAA+TGG	0.440359	6:-96375284	MsG0680034895.01.T01:CDS
AAAGGAGTGAAGCCAAGCAG+TGG	0.603258	6:-96374990	MsG0680034895.01.T01:CDS
AAATGTCTGTAAAGCACTGG+TGG	0.580192	6:+96379052	None:intergenic
AAGAAGCCAAGTATCCAAAT+GGG	0.544681	6:-96375283	MsG0680034895.01.T01:CDS
AAGATCAACTTCAGGAATGA+TGG	0.456137	6:+96375536	None:intergenic
AATCGAGAGGAGACAGTGAA+AGG	0.569341	6:-96375008	MsG0680034895.01.T01:CDS
AATGATGGACATGCGGTTCA+GGG	0.492877	6:-96378908	MsG0680034895.01.T01:CDS
AATGGGAACCGATCTGACAG+AGG	0.720450	6:-96375266	MsG0680034895.01.T01:CDS
ACAACAAGTTCTTCATCTGT+TGG	0.464543	6:+96375603	None:intergenic
ACTGAAAACAATTGTGTCAA+CGG	0.565450	6:+96378994	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGACAGTCTTAAATTAAG+AGG	+	Chr6:96375208-96375227	None:intergenic	30.0%
AAGGCGTAATATTAGGAATA+TGG	-	Chr6:96376263-96376282	MsG0680034895.01.T01:intron	30.0%
ACCTATGATAAAGCTCTATT+TGG	+	Chr6:96377426-96377445	None:intergenic	30.0%
ACTGAAAACAATTGTGTCAA+CGG	+	Chr6:96374663-96374682	None:intergenic	30.0%
AGCTTTGCTTGTCATTATTT+AGG	-	Chr6:96377515-96377534	MsG0680034895.01.T01:intron	30.0%
AGGACAGTCTTAAATTAAGA+GGG	+	Chr6:96375207-96375226	None:intergenic	30.0%
AGGCGTAATATTAGGAATAT+GGG	-	Chr6:96376264-96376283	MsG0680034895.01.T01:intron	30.0%
ATCTCAAATGTTCCAAAATG+AGG	-	Chr6:96378961-96378980	MsG0680034895.01.T01:CDS	30.0%
ATTTGTACCAATTGTCTAAG+GGG	-	Chr6:96375452-96375471	MsG0680034895.01.T01:intron	30.0%
CAATTCATTTGTGATTGAAG+AGG	+	Chr6:96378791-96378810	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	96374581	96379095	96374581	ID=MsG0680034895.01;Name=MsG0680034895.01
Chr6	mRNA	96374581	96379095	96374581	ID=MsG0680034895.01.T01;Parent=MsG0680034895.01;Name=MsG0680034895.01.T01;_AED=0.28;_eAED=0.28;_QI=0\|0.66\|0.5\|1\|0.33\|0.25\|4\|201\|351
Chr6	exon	96374581	96374781	96374581	ID=MsG0680034895.01.T01:exon:6859;Parent=MsG0680034895.01.T01
Chr6	exon	96374803	96375341	96374803	ID=MsG0680034895.01.T01:exon:6858;Parent=MsG0680034895.01.T01
Chr6	exon	96375508	96375748	96375508	ID=MsG0680034895.01.T01:exon:6857;Parent=MsG0680034895.01.T01
Chr6	exon	96378820	96379095	96378820	ID=MsG0680034895.01.T01:exon:6856;Parent=MsG0680034895.01.T01
Chr6	CDS	96378820	96379095	96378820	ID=MsG0680034895.01.T01:cds;Parent=MsG0680034895.01.T01
Chr6	CDS	96375508	96375748	96375508	ID=MsG0680034895.01.T01:cds;Parent=MsG0680034895.01.T01
Chr6	CDS	96374803	96375341	96374803	ID=MsG0680034895.01.T01:cds;Parent=MsG0680034895.01.T01
Chr6	three_prime_UTR	96374581	96374781	96374581	ID=MsG0680034895.01.T01:three_prime_utr;Parent=MsG0680034895.01.T01

Gene Sequence

>MsG0680034895.01.T01

ATGTCTGGTTGGAAAGCGACTCCACCAGTGCTTTACAGACATTTAAGAATGTGGTTATGGTTCCAATTCGTATTCGTAACCGTTGACACAATTGTTTTCAGTCAGGTATCCGTTGTTTCATCTTATATTTTGCGTGAAGGAAATGATTGTGCTCATAAGCTAGCGAATGATGGACATGCGGTTCAGGGTGCTTGGTGGTCAAATTCTTTACCGGAGTTTATTTATGACAAATGTGAAGTCTTAGATATGAATAGAGGTTTACTTGCAGAGTCTCGTAAGGCACACTTCATTTTACTCTTCTCCATGCCTTTTATTTGTAAAGATAAGGAAGATAAGATGGAGAAGATGAATTTTGTGAAGAATGGTGAAGTTAGATTGCCACCAGGTTTCCGTTTCCATCCAACAGATGAAGAACTTGTTGTTCAATACTTGAAGCGTAAGGTATTCTCTTTTCCTTTACCTGTTTCCATCATTCCTGAAGTTGATCTTTGCAAGTCTGATCCTTGGGATTTACCTGGTGATATGGAACAAGAGAGGTACTTTTTCAGTACAAAAGAAGCCAAGTATCCAAATGGGAACCGATCTGACAGAGGTACAAACTCTGGTTACTGGAAAGCAACTGGTTTGGACAAACAAATCATGAATTCAAAGACTCATGAAGTTGTTGGCATGAAGAAAACTCTTGTTTTCTACAGAGGCAAACCACCTCATGGTTCAAGAACTGATTGGATCATGCATGAATATCGCCTCACTTCCTCTCACTCCAACCCCTTGAATGAAAATTGGGTTCTGTATCGCATATTTCTGAAGAGGAGAGGTGGTGCTATAAATCGAGAGGAGACAGTGAAAGGAGTGAAGCCAAGCAGTGGCAGCAGTTTTGGTGAGGTGAAGAAGAACTCAGATTCAAGTTCAAAAGTGGTTGTTTTCTACGATTTCTTCGCAGAGAAGAAGAACAATTCTGATGCATCCTCCTCTTCAATCACAAATGAATTGGCTGAACATGAAAATGAAGACAGCAGTAACTCATTCACAGTGCTCCCATCCTTTTATTATTAG

Protein sequence

>MsG0680034895.01.T01

MSGWKATPPVLYRHLRMWLWFQFVFVTVDTIVFSQVSVVSSYILREGNDCAHKLANDGHAVQGAWWSNSLPEFIYDKCEVLDMNRGLLAESRKAHFILLFSMPFICKDKEDKMEKMNFVKNGEVRLPPGFRFHPTDEELVVQYLKRKVFSFPLPVSIIPEVDLCKSDPWDLPGDMEQERYFFSTKEAKYPNGNRSDRGTNSGYWKATGLDKQIMNSKTHEVVGMKKTLVFYRGKPPHGSRTDWIMHEYRLTSSHSNPLNENWVLYRIFLKRRGGAINREETVKGVKPSSGSSFGEVKKNSDSSSKVVVFYDFFAEKKNNSDASSSSITNELAEHENEDSSNSFTVLPSFYY*