Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035002.01.T01 | XP_024629942.1 | 86.425 | 221 | 30 | 0 | 1 | 221 | 1 | 221 | 2.92E-138 | 399 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035002.01.T01 | Q9SZL8 | 33.032 | 221 | 143 | 4 | 6 | 222 | 69 | 288 | 1.94E-32 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035002.01.T01 | A0A396H640 | 77.130 | 223 | 50 | 1 | 1 | 222 | 1 | 223 | 1.91e-125 | 366 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0680035002.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035002.01.T01 | MTR_7g074790 | 73.034 | 178 | 30 | 1 | 1 | 178 | 1 | 160 | 9.64e-89 | 261 |
| MsG0680035002.01.T01 | MTR_5g039760 | 61.165 | 206 | 55 | 2 | 1 | 205 | 1 | 182 | 1.25e-85 | 252 |
| MsG0680035002.01.T01 | MTR_7g061980 | 66.387 | 119 | 40 | 0 | 1 | 119 | 1 | 119 | 6.41e-56 | 174 |
| MsG0680035002.01.T01 | MTR_0402s0040 | 41.436 | 181 | 101 | 4 | 10 | 187 | 26 | 204 | 3.09e-33 | 125 |
| MsG0680035002.01.T01 | MTR_0558s0020 | 29.487 | 234 | 142 | 5 | 8 | 221 | 37 | 267 | 1.13e-29 | 112 |
| MsG0680035002.01.T01 | MTR_4g102720 | 34.911 | 169 | 76 | 2 | 7 | 175 | 4 | 138 | 6.13e-29 | 108 |
| MsG0680035002.01.T01 | MTR_1g083640 | 28.632 | 234 | 144 | 6 | 8 | 221 | 52 | 282 | 8.83e-28 | 107 |
| MsG0680035002.01.T01 | MTR_6g051425 | 29.333 | 225 | 145 | 6 | 9 | 222 | 13 | 234 | 5.72e-26 | 103 |
| MsG0680035002.01.T01 | MTR_8g446020 | 28.704 | 216 | 128 | 6 | 8 | 221 | 36 | 227 | 1.75e-22 | 92.4 |
| MsG0680035002.01.T01 | MTR_4g069880 | 58.824 | 68 | 28 | 0 | 1 | 68 | 1 | 68 | 1.13e-21 | 85.1 |
| MsG0680035002.01.T01 | MTR_0337s0040 | 28.230 | 209 | 127 | 4 | 11 | 217 | 37 | 224 | 6.86e-19 | 82.0 |
| MsG0680035002.01.T01 | MTR_1g019850 | 33.566 | 143 | 82 | 4 | 3 | 141 | 63 | 196 | 8.18e-19 | 82.0 |
| MsG0680035002.01.T01 | MTR_6g090505 | 29.016 | 193 | 121 | 4 | 45 | 221 | 10 | 202 | 1.44e-18 | 83.2 |
| MsG0680035002.01.T01 | MTR_5g015480 | 37.179 | 156 | 84 | 5 | 4 | 156 | 53 | 197 | 6.85e-18 | 80.1 |
| MsG0680035002.01.T01 | MTR_1g013060 | 28.365 | 208 | 142 | 3 | 7 | 208 | 21 | 227 | 1.75e-17 | 78.6 |
| MsG0680035002.01.T01 | MTR_2g099430 | 44.898 | 98 | 50 | 3 | 25 | 119 | 16 | 112 | 2.13e-17 | 75.5 |
| MsG0680035002.01.T01 | MTR_1g070355 | 25.118 | 211 | 151 | 3 | 15 | 221 | 79 | 286 | 2.28e-17 | 80.5 |
| MsG0680035002.01.T01 | MTR_2g014100 | 26.471 | 204 | 127 | 6 | 8 | 191 | 45 | 245 | 2.39e-16 | 75.9 |
| MsG0680035002.01.T01 | MTR_7g113010 | 40.945 | 127 | 59 | 5 | 10 | 134 | 457 | 569 | 3.28e-16 | 77.4 |
| MsG0680035002.01.T01 | MTR_5g076070 | 24.528 | 212 | 155 | 3 | 13 | 221 | 56 | 265 | 3.04e-14 | 70.5 |
| MsG0680035002.01.T01 | MTR_2g070590 | 41.346 | 104 | 57 | 3 | 10 | 110 | 17 | 119 | 6.47e-14 | 66.2 |
| MsG0680035002.01.T01 | MTR_6g017200 | 45.783 | 83 | 43 | 2 | 10 | 91 | 58 | 139 | 6.62e-14 | 67.0 |
| MsG0680035002.01.T01 | MTR_0853s0010 | 27.586 | 145 | 89 | 4 | 5 | 141 | 22 | 158 | 4.42e-13 | 65.1 |
| MsG0680035002.01.T01 | MTR_7g446520 | 25.000 | 208 | 141 | 3 | 14 | 221 | 92 | 284 | 4.18e-12 | 64.7 |
| MsG0680035002.01.T01 | MTR_1g055240 | 32.456 | 114 | 75 | 1 | 77 | 188 | 32 | 145 | 1.48e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035002.01.T01 | AT4G38180 | 33.032 | 221 | 143 | 4 | 6 | 222 | 69 | 288 | 1.98e-33 | 127 |
| MsG0680035002.01.T01 | AT4G15090 | 31.390 | 223 | 148 | 5 | 3 | 222 | 44 | 264 | 3.11e-27 | 109 |
| MsG0680035002.01.T01 | AT4G15090 | 31.390 | 223 | 148 | 5 | 3 | 222 | 44 | 264 | 3.11e-27 | 109 |
| MsG0680035002.01.T01 | AT4G15090 | 31.390 | 223 | 148 | 5 | 3 | 222 | 44 | 264 | 3.11e-27 | 109 |
| MsG0680035002.01.T01 | AT4G15090 | 31.390 | 223 | 148 | 5 | 3 | 222 | 44 | 264 | 3.11e-27 | 109 |
| MsG0680035002.01.T01 | AT4G15090 | 31.390 | 223 | 148 | 5 | 3 | 222 | 44 | 264 | 3.11e-27 | 109 |
| MsG0680035002.01.T01 | AT1G76320 | 29.717 | 212 | 135 | 6 | 14 | 222 | 1 | 201 | 3.06e-17 | 80.5 |
| MsG0680035002.01.T01 | AT1G76320 | 29.717 | 212 | 135 | 6 | 14 | 222 | 1 | 201 | 3.41e-17 | 80.1 |
| MsG0680035002.01.T01 | AT1G76320 | 29.717 | 212 | 135 | 6 | 14 | 222 | 1 | 201 | 3.58e-17 | 80.1 |
| MsG0680035002.01.T01 | AT1G76320 | 29.717 | 212 | 135 | 6 | 14 | 222 | 1 | 201 | 3.58e-17 | 80.1 |
| MsG0680035002.01.T01 | AT2G27110 | 28.037 | 214 | 128 | 5 | 9 | 222 | 48 | 235 | 6.11e-15 | 73.6 |
| MsG0680035002.01.T01 | AT2G27110 | 28.037 | 214 | 128 | 5 | 9 | 222 | 48 | 235 | 6.11e-15 | 73.6 |
| MsG0680035002.01.T01 | AT3G06250 | 26.852 | 216 | 116 | 5 | 9 | 222 | 189 | 364 | 1.22e-14 | 72.8 |
| MsG0680035002.01.T01 | AT3G06250 | 38.462 | 104 | 48 | 3 | 9 | 110 | 27 | 116 | 3.28e-12 | 65.9 |
| MsG0680035002.01.T01 | AT3G06250 | 26.852 | 216 | 116 | 5 | 9 | 222 | 189 | 364 | 1.22e-14 | 72.8 |
| MsG0680035002.01.T01 | AT3G06250 | 38.462 | 104 | 48 | 3 | 9 | 110 | 27 | 116 | 3.28e-12 | 65.9 |
| MsG0680035002.01.T01 | AT3G06250 | 26.852 | 216 | 116 | 5 | 9 | 222 | 189 | 364 | 1.22e-14 | 72.8 |
| MsG0680035002.01.T01 | AT3G06250 | 38.462 | 104 | 48 | 3 | 9 | 110 | 27 | 116 | 3.28e-12 | 65.9 |
| MsG0680035002.01.T01 | AT3G22170 | 24.675 | 231 | 137 | 5 | 6 | 222 | 66 | 273 | 3.48e-13 | 68.6 |
| MsG0680035002.01.T01 | AT3G22170 | 24.675 | 231 | 137 | 5 | 6 | 222 | 66 | 273 | 3.48e-13 | 68.6 |
| MsG0680035002.01.T01 | AT5G18960 | 39.423 | 104 | 47 | 3 | 9 | 110 | 42 | 131 | 1.08e-12 | 67.0 |
| MsG0680035002.01.T01 | AT5G18960 | 39.423 | 104 | 47 | 3 | 9 | 110 | 42 | 131 | 1.08e-12 | 67.0 |
| MsG0680035002.01.T01 | AT5G28530 | 25.444 | 169 | 121 | 2 | 8 | 171 | 53 | 221 | 1.45e-12 | 66.6 |
| MsG0680035002.01.T01 | AT5G28530 | 25.444 | 169 | 121 | 2 | 8 | 171 | 53 | 221 | 1.48e-12 | 66.6 |
| MsG0680035002.01.T01 | AT5G28530 | 25.444 | 169 | 121 | 2 | 8 | 171 | 53 | 221 | 1.48e-12 | 66.6 |
| MsG0680035002.01.T01 | AT5G28530 | 25.444 | 169 | 121 | 2 | 8 | 171 | 53 | 221 | 1.70e-12 | 66.6 |
| MsG0680035002.01.T01 | AT5G28530 | 25.444 | 169 | 121 | 2 | 8 | 171 | 53 | 221 | 1.78e-12 | 66.2 |
Find 35 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACACTTTCACGTGAAAA+TGG | 0.225368 | 6:-97604738 | MsG0680035002.01.T01:CDS |
| TGGAAGAGGCTGAACAATTT+TGG | 0.228865 | 6:-97604944 | MsG0680035002.01.T01:CDS |
| TGGAATGAAATTTGATACTA+TGG | 0.288067 | 6:-97604964 | MsG0680035002.01.T01:CDS |
| AACAAATTACAAACGTGTTC+TGG | 0.334571 | 6:-97604368 | MsG0680035002.01.T01:CDS |
| ATGGCTGATGACTCTGGATT+AGG | 0.348989 | 6:-97604597 | MsG0680035002.01.T01:CDS |
| TGACATCTTCAAATGTTGAT+TGG | 0.374678 | 6:-97604998 | MsG0680035002.01.T01:CDS |
| TGGCTGATGACTCTGGATTA+GGG | 0.390441 | 6:-97604596 | MsG0680035002.01.T01:CDS |
| TCAAATTTCATTCCAAGTCT+TGG | 0.395389 | 6:+97604972 | None:intergenic |
| GTATTTCCAACAGCAATTGT+TGG | 0.433256 | 6:-97604433 | MsG0680035002.01.T01:CDS |
| ATGTAGGTTTGTTTGTTGTA+AGG | 0.436082 | 6:-97604844 | MsG0680035002.01.T01:CDS |
| ACAAGCAAATGAGATTGATA+TGG | 0.437582 | 6:-97604616 | MsG0680035002.01.T01:CDS |
| CCGTTCTAGACTCGGCTCTC+CGG | 0.443682 | 6:+97604775 | None:intergenic |
| ATTGATATGGCTGATGACTC+TGG | 0.443968 | 6:-97604603 | MsG0680035002.01.T01:CDS |
| AGGTTTGTTTGTTGTAAGGA+AGG | 0.486878 | 6:-97604840 | MsG0680035002.01.T01:CDS |
| GTTGATTGGAAGCCAAGACT+TGG | 0.497681 | 6:-97604984 | MsG0680035002.01.T01:CDS |
| CTTCTTTCATGCATATCAGA+TGG | 0.507819 | 6:-97604400 | MsG0680035002.01.T01:CDS |
| AGACAAAGAAGCATGGTGTA+TGG | 0.510301 | 6:-97604477 | MsG0680035002.01.T01:CDS |
| GATTCTCCAACAATTGCTGT+TGG | 0.523496 | 6:+97604427 | None:intergenic |
| TTCACGTGAAAATGGAAAGC+TGG | 0.524682 | 6:-97604730 | MsG0680035002.01.T01:CDS |
| GAAATTTGATACTATGGAAG+AGG | 0.525500 | 6:-97604958 | MsG0680035002.01.T01:CDS |
| AGCATGGTGTATGGCGACGC+TGG | 0.534307 | 6:-97604468 | MsG0680035002.01.T01:CDS |
| CAACTTATGAGCACACATGC+AGG | 0.537532 | 6:-97604558 | MsG0680035002.01.T01:CDS |
| TAAGCAATCCGTTCTAGACT+CGG | 0.542410 | 6:+97604767 | None:intergenic |
| CATCTGATATGCATGAAAGA+AGG | 0.546601 | 6:+97604401 | None:intergenic |
| CCGGAGAGCCGAGTCTAGAA+CGG | 0.566334 | 6:-97604775 | MsG0680035002.01.T01:CDS |
| TTCTATGTGATCGAAGCATG+TGG | 0.582014 | 6:+97604652 | None:intergenic |
| GCAGGGCATAGAGCTAATGT+TGG | 0.592621 | 6:-97604540 | MsG0680035002.01.T01:CDS |
| AACTTATGAGCACACATGCA+GGG | 0.596381 | 6:-97604557 | MsG0680035002.01.T01:CDS |
| ACGGCGATGTATCGTCATGT+AGG | 0.604688 | 6:-97604860 | MsG0680035002.01.T01:CDS |
| TGACTCTGGATTAGGGCAGA+AGG | 0.608129 | 6:-97604589 | MsG0680035002.01.T01:CDS |
| TTTGTTGTAAGGAAGGACTA+CGG | 0.614427 | 6:-97604833 | MsG0680035002.01.T01:CDS |
| TGATATGCATGAAAGAAGGA+CGG | 0.649185 | 6:+97604405 | None:intergenic |
| TCTATGTGATCGAAGCATGT+GGG | 0.671774 | 6:+97604653 | None:intergenic |
| TAATGTTGGATATACAGAAG+TGG | 0.683641 | 6:-97604526 | MsG0680035002.01.T01:CDS |
| TTTACAAACAAGAACAAACA+CGG | 0.696353 | 6:-97604879 | MsG0680035002.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTTTTTCTTTAAACTCA+TGG | + | Chr6:97604652-97604671 | None:intergenic | 20.0% |
| ! | GAGAAAAAATATGCATATGA+AGG | - | Chr6:97604548-97604567 | MsG0680035002.01.T01:CDS | 25.0% |
| ! | TGGAATGAAATTTGATACTA+TGG | - | Chr6:97604388-97604407 | MsG0680035002.01.T01:CDS | 25.0% |
| ! | TTTACAAACAAGAACAAACA+CGG | - | Chr6:97604473-97604492 | MsG0680035002.01.T01:CDS | 25.0% |
| AACAAATTACAAACGTGTTC+TGG | - | Chr6:97604984-97605003 | MsG0680035002.01.T01:CDS | 30.0% | |
| ACAAGCAAATGAGATTGATA+TGG | - | Chr6:97604736-97604755 | MsG0680035002.01.T01:CDS | 30.0% | |
| ATGCATATGAAGGAAAAATC+CGG | - | Chr6:97604558-97604577 | MsG0680035002.01.T01:CDS | 30.0% | |
| ATTACACTTTCACGTGAAAA+TGG | - | Chr6:97604614-97604633 | MsG0680035002.01.T01:CDS | 30.0% | |
| GAAATTTGATACTATGGAAG+AGG | - | Chr6:97604394-97604413 | MsG0680035002.01.T01:CDS | 30.0% | |
| TAATGTTGGATATACAGAAG+TGG | - | Chr6:97604826-97604845 | MsG0680035002.01.T01:CDS | 30.0% | |
| TACAAAAAGACAAAGAAGCA+TGG | - | Chr6:97604868-97604887 | MsG0680035002.01.T01:CDS | 30.0% | |
| TCAAATTTCATTCCAAGTCT+TGG | + | Chr6:97604383-97604402 | None:intergenic | 30.0% | |
| TGACATCTTCAAATGTTGAT+TGG | - | Chr6:97604354-97604373 | MsG0680035002.01.T01:CDS | 30.0% | |
| !! | ATGTAGGTTTGTTTGTTGTA+AGG | - | Chr6:97604508-97604527 | MsG0680035002.01.T01:CDS | 30.0% |
| CATCTGATATGCATGAAAGA+AGG | + | Chr6:97604954-97604973 | None:intergenic | 35.0% | |
| CTTCTTTCATGCATATCAGA+TGG | - | Chr6:97604952-97604971 | MsG0680035002.01.T01:CDS | 35.0% | |
| GTATTTCCAACAGCAATTGT+TGG | - | Chr6:97604919-97604938 | MsG0680035002.01.T01:CDS | 35.0% | |
| TGATATGCATGAAAGAAGGA+CGG | + | Chr6:97604950-97604969 | None:intergenic | 35.0% | |
| ! | TTTGTTGTAAGGAAGGACTA+CGG | - | Chr6:97604519-97604538 | MsG0680035002.01.T01:CDS | 35.0% |
| !! | AGGTTTGTTTGTTGTAAGGA+AGG | - | Chr6:97604512-97604531 | MsG0680035002.01.T01:CDS | 35.0% |
| !! | TCTCTCGTTTTAGTAGATCA+TGG | + | Chr6:97604676-97604695 | None:intergenic | 35.0% |
| !!! | GAACAATTTTGGCTTGCTTA+CGG | - | Chr6:97604419-97604438 | MsG0680035002.01.T01:CDS | 35.0% |
| AACTTATGAGCACACATGCA+GGG | - | Chr6:97604795-97604814 | MsG0680035002.01.T01:CDS | 40.0% | |
| ATTGATATGGCTGATGACTC+TGG | - | Chr6:97604749-97604768 | MsG0680035002.01.T01:CDS | 40.0% | |
| GATTCTCCAACAATTGCTGT+TGG | + | Chr6:97604928-97604947 | None:intergenic | 40.0% | |
| TCTATGTGATCGAAGCATGT+GGG | + | Chr6:97604702-97604721 | None:intergenic | 40.0% | |
| TTCACGTGAAAATGGAAAGC+TGG | - | Chr6:97604622-97604641 | MsG0680035002.01.T01:CDS | 40.0% | |
| TTCTATGTGATCGAAGCATG+TGG | + | Chr6:97604703-97604722 | None:intergenic | 40.0% | |
| ! | AGACAAAGAAGCATGGTGTA+TGG | - | Chr6:97604875-97604894 | MsG0680035002.01.T01:CDS | 40.0% |
| ! | TAAGCAATCCGTTCTAGACT+CGG | + | Chr6:97604588-97604607 | None:intergenic | 40.0% |
| ! | TGGAAGAGGCTGAACAATTT+TGG | - | Chr6:97604408-97604427 | MsG0680035002.01.T01:CDS | 40.0% |
| ATGGCTGATGACTCTGGATT+AGG | - | Chr6:97604755-97604774 | MsG0680035002.01.T01:CDS | 45.0% | |
| CAACTTATGAGCACACATGC+AGG | - | Chr6:97604794-97604813 | MsG0680035002.01.T01:CDS | 45.0% | |
| GTTGATTGGAAGCCAAGACT+TGG | - | Chr6:97604368-97604387 | MsG0680035002.01.T01:CDS | 45.0% | |
| TGGCTGATGACTCTGGATTA+GGG | - | Chr6:97604756-97604775 | MsG0680035002.01.T01:CDS | 45.0% | |
| ! | CTTGCTTACGGTTTTCGTGA+GGG | - | Chr6:97604431-97604450 | MsG0680035002.01.T01:CDS | 45.0% |
| ACGGCGATGTATCGTCATGT+AGG | - | Chr6:97604492-97604511 | MsG0680035002.01.T01:CDS | 50.0% | |
| GCAGGGCATAGAGCTAATGT+TGG | - | Chr6:97604812-97604831 | MsG0680035002.01.T01:CDS | 50.0% | |
| ! | GCTTGCTTACGGTTTTCGTG+AGG | - | Chr6:97604430-97604449 | MsG0680035002.01.T01:CDS | 50.0% |
| ! | TACGGTTTTCGTGAGGGCTT+CGG | - | Chr6:97604437-97604456 | MsG0680035002.01.T01:CDS | 50.0% |
| ! | TGACTCTGGATTAGGGCAGA+AGG | - | Chr6:97604763-97604782 | MsG0680035002.01.T01:CDS | 50.0% |
| AGCATGGTGTATGGCGACGC+TGG | - | Chr6:97604884-97604903 | MsG0680035002.01.T01:CDS | 60.0% | |
| CCGGAGAGCCGAGTCTAGAA+CGG | - | Chr6:97604577-97604596 | MsG0680035002.01.T01:CDS | 60.0% | |
| ! | CCGTTCTAGACTCGGCTCTC+CGG | + | Chr6:97604580-97604599 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 97604353 | 97605021 | 97604353 | ID=MsG0680035002.01;Name=MsG0680035002.01 |
| Chr6 | mRNA | 97604353 | 97605021 | 97604353 | ID=MsG0680035002.01.T01;Parent=MsG0680035002.01;Name=MsG0680035002.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|222 |
| Chr6 | exon | 97604353 | 97605021 | 97604353 | ID=MsG0680035002.01.T01:exon:13855;Parent=MsG0680035002.01.T01 |
| Chr6 | CDS | 97604353 | 97605021 | 97604353 | ID=MsG0680035002.01.T01:cds;Parent=MsG0680035002.01.T01 |
| Gene Sequence |
| Protein sequence |
