AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035562.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035562.01.T01 MTR_3g072200 61.364 176 34 3 2 144 8 182 3.90e-60 195
MsG0680035562.01.T01 MTR_4g049670 71.756 131 24 2 1 118 1 131 8.33e-60 182
MsG0680035562.01.T01 MTR_1g058140 62.821 156 35 2 1 144 22 166 9.73e-59 183
MsG0680035562.01.T01 MTR_3g462670 61.842 152 50 1 1 144 1 152 1.27e-57 179
MsG0680035562.01.T01 MTR_2g067930 66.061 165 35 2 1 144 1 165 2.91e-57 177
MsG0680035562.01.T01 MTR_1g081740 63.190 163 40 4 2 144 7 169 4.37e-56 174
MsG0680035562.01.T01 MTR_2g026585 60.976 164 42 2 2 144 8 170 2.42e-53 173
MsG0680035562.01.T01 MTR_3g030230 66.667 123 38 1 22 144 44 163 3.07e-52 164
MsG0680035562.01.T01 MTR_3g015530 82.105 95 17 0 50 144 92 186 1.53e-51 165
MsG0680035562.01.T01 MTR_7g050385 56.790 162 20 1 11 122 68 229 1.30e-50 162
MsG0680035562.01.T01 MTR_1g059880 67.176 131 21 2 2 110 44 174 3.93e-50 160
MsG0680035562.01.T01 MTR_4g051638 55.233 172 15 2 2 123 80 239 6.08e-50 160
MsG0680035562.01.T01 MTR_4g057300 76.344 93 22 0 52 144 1 93 6.09e-49 161
MsG0680035562.01.T01 MTR_4g113170 64.336 143 34 1 2 144 8 133 8.25e-46 159
MsG0680035562.01.T01 MTR_6g089230 63.964 111 37 1 44 154 15 122 1.33e-43 144
MsG0680035562.01.T01 MTR_6g026910 74.257 101 15 2 2 97 8 102 4.56e-43 143
MsG0680035562.01.T01 MTR_8g038805 52.985 134 59 2 1 132 1 132 5.60e-42 137
MsG0680035562.01.T01 MTR_7g106560 57.143 147 43 4 11 144 13 152 1.28e-40 135
MsG0680035562.01.T01 MTR_6g075990 42.347 196 61 2 1 144 19 214 1.45e-39 134
MsG0680035562.01.T01 MTR_8g009330 38.498 213 77 3 1 163 24 232 1.39e-38 131
MsG0680035562.01.T01 MTR_5g031650 59.596 99 40 0 46 144 120 218 2.03e-37 128
MsG0680035562.01.T01 MTR_4g119600 41.327 196 63 3 1 144 1 196 4.44e-37 130
MsG0680035562.01.T01 MTR_8g107490 55.357 112 47 1 33 144 67 175 8.84e-37 128
MsG0680035562.01.T01 MTR_8g445570 73.611 72 19 0 73 144 4 75 1.49e-34 117
MsG0680035562.01.T01 MTR_3g067290 43.860 171 69 1 1 144 28 198 9.85e-33 116
MsG0680035562.01.T01 MTR_4g057975 39.474 190 69 5 1 144 1 190 4.30e-32 114
MsG0680035562.01.T01 MTR_7g050365 66.667 87 5 1 58 144 20 82 9.71e-31 110
MsG0680035562.01.T01 MTR_3g437000 50.000 126 19 2 15 96 5 130 3.13e-30 107
MsG0680035562.01.T01 MTR_5g060510 60.331 121 22 1 22 116 38 158 2.66e-29 106
MsG0680035562.01.T01 MTR_8g103800 39.157 166 79 3 1 144 1 166 2.69e-29 108
MsG0680035562.01.T01 MTR_6g090600 63.158 76 28 0 52 127 27 102 1.43e-28 102
MsG0680035562.01.T01 MTR_3g077875 47.525 101 51 1 46 144 116 216 2.26e-28 106
MsG0680035562.01.T01 MTR_7g060350 41.143 175 71 5 1 144 1 174 2.95e-27 100
MsG0680035562.01.T01 MTR_3g087440 45.833 120 62 2 46 162 116 235 3.01e-27 102
MsG0680035562.01.T01 MTR_5g060540 43.949 157 43 2 25 146 10 156 3.73e-27 105
MsG0680035562.01.T01 MTR_8g020433 39.474 152 79 2 1 144 1 147 3.41e-26 103
MsG0680035562.01.T01 MTR_6g464570 51.111 90 42 2 53 142 65 152 1.30e-24 93.6
MsG0680035562.01.T01 MTR_1g080040 46.602 103 53 1 53 153 1 103 3.11e-23 89.7
MsG0680035562.01.T01 MTR_5g006100 45.000 100 53 1 47 144 93 192 1.23e-22 94.0
MsG0680035562.01.T01 MTR_6g084740 44.755 143 53 5 2 144 8 124 2.91e-21 85.1
MsG0680035562.01.T01 MTR_1g098990 37.349 166 81 4 1 144 1 165 3.91e-21 87.4
MsG0680035562.01.T01 MTR_7g010970 46.667 90 45 2 57 144 6 94 8.89e-21 85.5
MsG0680035562.01.T01 MTR_0156s0040 47.368 95 48 1 52 144 1 95 1.27e-20 84.0
MsG0680035562.01.T01 MTR_2g451070 45.902 122 10 2 1 72 7 122 2.23e-20 81.6
MsG0680035562.01.T01 MTR_3g034840 42.708 96 53 1 51 144 111 206 6.97e-20 82.8
MsG0680035562.01.T01 MTR_0599s0020 42.708 96 53 1 51 144 111 206 6.97e-20 82.8
MsG0680035562.01.T01 MTR_5g046245 62.903 62 23 0 83 144 5 66 3.23e-19 81.3
MsG0680035562.01.T01 MTR_5g046245 49.351 77 39 0 86 162 152 228 1.37e-18 79.7
MsG0680035562.01.T01 MTR_6g029530 42.268 97 55 1 55 151 553 648 5.32e-19 83.2
MsG0680035562.01.T01 MTR_7g034570 32.000 150 87 3 2 144 18 159 7.81e-19 79.3
MsG0680035562.01.T01 MTR_5g070810 41.053 95 51 2 37 129 21 112 8.24e-19 77.4
MsG0680035562.01.T01 MTR_1g012760 41.176 102 56 2 43 144 71 168 1.10e-18 80.9
MsG0680035562.01.T01 MTR_1g012760 41.176 102 56 2 43 144 82 179 1.61e-18 80.5
MsG0680035562.01.T01 MTR_5g046160 36.607 112 53 2 33 144 68 161 1.82e-18 79.7
MsG0680035562.01.T01 MTR_1g088955 40.458 131 52 6 1 130 1 106 3.17e-17 74.3
MsG0680035562.01.T01 MTR_2g096070 41.071 112 33 2 33 144 106 184 4.07e-17 77.8
MsG0680035562.01.T01 MTR_7g065910 41.489 94 44 2 53 144 34 118 3.99e-16 74.7
MsG0680035562.01.T01 MTR_3g467720 36.111 144 58 3 1 144 7 116 4.78e-16 70.9
MsG0680035562.01.T01 MTR_0116s0050 51.613 62 30 0 83 144 82 143 1.44e-15 70.5
MsG0680035562.01.T01 MTR_1g052890 59.322 59 24 0 86 144 2 60 1.87e-15 72.4
MsG0680035562.01.T01 MTR_1g043340 52.459 61 29 0 99 159 3 63 9.96e-15 66.6
MsG0680035562.01.T01 MTR_7g024830 85.714 35 5 0 110 144 1 35 1.73e-14 65.1
MsG0680035562.01.T01 MTR_3g116710 32.632 95 63 1 50 144 124 217 1.56e-13 66.2
MsG0680035562.01.T01 MTR_2g034775 64.179 67 11 3 2 67 37 91 2.08e-13 65.9
MsG0680035562.01.T01 MTR_5g079560 42.529 87 45 2 46 127 112 198 2.13e-13 65.9
MsG0680035562.01.T01 MTR_5g071980 72.917 48 7 1 2 49 8 49 1.09e-12 64.3
MsG0680035562.01.T01 MTR_4g066090 50.000 66 33 0 88 153 70 135 1.28e-12 62.4
MsG0680035562.01.T01 MTR_7g064870 26.115 157 80 3 1 121 1 157 3.41e-12 61.6
MsG0680035562.01.T01 MTR_5g055912 56.522 46 20 0 89 134 16 61 3.51e-12 58.5
MsG0680035562.01.T01 MTR_8g446840 44.156 77 38 2 68 144 1 72 4.19e-12 59.3
MsG0680035562.01.T01 MTR_5g046145 30.303 99 68 1 56 154 162 259 2.54e-11 60.8
MsG0680035562.01.T01 MTR_6g081290 36.458 96 49 2 53 143 3 91 3.49e-11 57.0
MsG0680035562.01.T01 MTR_2g461140 40.217 92 26 3 53 144 91 153 3.68e-11 58.9
MsG0680035562.01.T01 MTR_2g090505 31.579 95 64 1 50 144 129 222 6.94e-11 59.7
MsG0680035562.01.T01 MTR_0528s0010 34.524 84 54 1 61 144 1 83 7.05e-11 57.4
MsG0680035562.01.T01 MTR_4g120790 40.984 61 36 0 84 144 110 170 9.36e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAATTGGCTATTCTTAA+TGG 0.154142 6:-108478009 MsG0680035562.01.T01:CDS
CCTTCTTGCCGGAAGATTAA+TGG 0.223899 6:-108477947 MsG0680035562.01.T01:CDS
CACGGAAGCTTCGACCTTAA+TGG 0.246908 6:+108478372 None:intergenic
GGAAGACAGTTCTTGGAAAT+TGG 0.278443 6:-108478022 MsG0680035562.01.T01:CDS
GTCGTCCTCCATTAATCTTC+CGG 0.328172 6:+108477939 None:intergenic
ACGGAAGCTTCGACCTTAAT+GGG 0.340420 6:+108478373 None:intergenic
ACATGACTTGACCAATTCTT+TGG 0.344961 6:-108477905 MsG0680035562.01.T01:CDS
GCTTGAAGCCTCAACTTTAA+CGG 0.401956 6:+108478336 None:intergenic
TAAGGTTGAAGCTCCCATTA+AGG 0.417279 6:-108478386 MsG0680035562.01.T01:CDS
TCGTCAGGGAAGACAGTTCT+TGG 0.424715 6:-108478029 MsG0680035562.01.T01:CDS
TGAGAATGCGGGTGATGATC+CGG 0.429344 6:-108478440 MsG0680035562.01.T01:CDS
AAGTGTTGTCGATTCAGTTA+AGG 0.435551 6:-108478404 MsG0680035562.01.T01:CDS
AGGTGCGTGGATATGTCGTC+AGG 0.438521 6:-108478044 MsG0680035562.01.T01:CDS
AACGGAAGCTTCAACCTTCA+CGG 0.456661 6:+108478354 None:intergenic
GTAGCAGGGAACCTTCTTGC+CGG 0.456891 6:-108477958 MsG0680035562.01.T01:CDS
TTAACTGAATCGACAACACT+TGG 0.482688 6:+108478406 None:intergenic
AGAAGGTTCCCTGCTACATA+TGG 0.486745 6:+108477964 None:intergenic
GAGATGGTGCCATATGTAGC+AGG 0.499746 6:-108477973 MsG0680035562.01.T01:CDS
ATAGATATGGTTGAGAATGC+GGG 0.504029 6:-108478451 None:intergenic
GACTTGCTTGATATTTGTCA+TGG 0.506902 6:+108477836 None:intergenic
TATAGATATGGTTGAGAATG+CGG 0.513416 6:-108478452 None:intergenic
CCATTAATCTTCCGGCAAGA+AGG 0.516371 6:+108477947 None:intergenic
CTACATATGGCACCATCTCA+TGG 0.520860 6:+108477977 None:intergenic
AAAATGTGGGTGTTTGTTCA+AGG 0.526388 6:-108478064 MsG0680035562.01.T01:CDS
AAAGTGAAGCATGAAGCAAC+GGG 0.536859 6:-108478093 MsG0680035562.01.T01:CDS
GAGAATGCGGGTGATGATCC+GGG 0.555681 6:-108478439 MsG0680035562.01.T01:CDS
TAGAAATGCTGTGTTGGAGT+TGG 0.560288 6:-108478154 MsG0680035562.01.T01:CDS
TGAAGCTTCCGTTAAAGTTG+AGG 0.561546 6:-108478344 MsG0680035562.01.T01:CDS
GCAACGGGATCAAGAAAATG+TGG 0.566912 6:-108478078 MsG0680035562.01.T01:CDS
TGTTGGAGTTGGTTTGTGAA+CGG 0.576271 6:-108478143 MsG0680035562.01.T01:CDS
ACACTTGGGTTTCGATCACC+CGG 0.577593 6:+108478421 None:intergenic
AAAAGTGAAGCATGAAGCAA+CGG 0.578845 6:-108478094 MsG0680035562.01.T01:CDS
TAACTGAATCGACAACACTT+GGG 0.597416 6:+108478407 None:intergenic
TGAAGGTAGAAATGCTGTGT+TGG 0.597946 6:-108478160 MsG0680035562.01.T01:intron
GGGTGTTTGTTCAAGGTGCG+TGG 0.617723 6:-108478057 MsG0680035562.01.T01:CDS
TGGCGTTCACAACCATGAGA+TGG 0.631982 6:-108477989 MsG0680035562.01.T01:CDS
TAAGGTCGAAGCTTCCGTGA+AGG 0.633123 6:-108478368 MsG0680035562.01.T01:CDS
CAACGGGATCAAGAAAATGT+GGG 0.655168 6:-108478077 MsG0680035562.01.T01:CDS
AGATGGTGCCATATGTAGCA+GGG 0.658748 6:-108477972 MsG0680035562.01.T01:CDS
GGTGCGTGGATATGTCGTCA+GGG 0.664523 6:-108478043 MsG0680035562.01.T01:CDS
TCTTGCCGGAAGATTAATGG+AGG 0.684933 6:-108477944 MsG0680035562.01.T01:CDS
GAGTTGGTTTGTGAACGGAG+TGG 0.704384 6:-108478138 MsG0680035562.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAATTTTTCAATATTTTT+TGG + Chr6:108478399-108478418 None:intergenic 10.0%
!! TGAAAAATTTGAAGAAGAAA+AGG - Chr6:108478407-108478426 MsG0680035562.01.T01:CDS 20.0%
! AAAGATAAAAACGAATTGCT+CGG - Chr6:108478033-108478052 MsG0680035562.01.T01:CDS 25.0%
!!! TTTTTTTTACCTGAGATACA+TGG - Chr6:108478010-108478029 MsG0680035562.01.T01:CDS 25.0%
ATCATTAGAAGATCTTGTGA+AGG - Chr6:108478096-108478115 MsG0680035562.01.T01:CDS 30.0%
TGGAAATTGGCTATTCTTAA+TGG - Chr6:108478264-108478283 MsG0680035562.01.T01:intron 30.0%
TTTATCTTTCCATGTATCTC+AGG + Chr6:108478022-108478041 None:intergenic 30.0%
!!! TTTTGGTTTCACCAAAGAAT+TGG + Chr6:108478382-108478401 None:intergenic 30.0%
AAAAGTGAAGCATGAAGCAA+CGG - Chr6:108478179-108478198 MsG0680035562.01.T01:intron 35.0%
AAAATGTGGGTGTTTGTTCA+AGG - Chr6:108478209-108478228 MsG0680035562.01.T01:intron 35.0%
ACATGACTTGACCAATTCTT+TGG - Chr6:108478368-108478387 MsG0680035562.01.T01:CDS 35.0%
AGCAAATAGGGCTGAATTTA+CGG - Chr6:108478071-108478090 MsG0680035562.01.T01:CDS 35.0%
ATAAAAACGAATTGCTCGGT+TGG - Chr6:108478037-108478056 MsG0680035562.01.T01:CDS 35.0%
GAAACTTCTTGACAATGAAG+TGG - Chr6:108477977-108477996 MsG0680035562.01.T01:CDS 35.0%
TAAAAACGAATTGCTCGGTT+GGG - Chr6:108478038-108478057 MsG0680035562.01.T01:CDS 35.0%
TAACTGAATCGACAACACTT+GGG + Chr6:108477869-108477888 None:intergenic 35.0%
TTAACTGAATCGACAACACT+TGG + Chr6:108477870-108477889 None:intergenic 35.0%
! GACTTGCTTGATATTTGTCA+TGG + Chr6:108478440-108478459 None:intergenic 35.0%
!! AAGTGTTGTCGATTCAGTTA+AGG - Chr6:108477869-108477888 MsG0680035562.01.T01:CDS 35.0%
!! TCATGCTTCACTTTTGTCTT+CGG + Chr6:108478174-108478193 None:intergenic 35.0%
AAAGTGAAGCATGAAGCAAC+GGG - Chr6:108478180-108478199 MsG0680035562.01.T01:intron 40.0%
CAACGGGATCAAGAAAATGT+GGG - Chr6:108478196-108478215 MsG0680035562.01.T01:intron 40.0%
GCTTGAAGCCTCAACTTTAA+CGG + Chr6:108477940-108477959 None:intergenic 40.0%
GGAAGACAGTTCTTGGAAAT+TGG - Chr6:108478251-108478270 MsG0680035562.01.T01:intron 40.0%
GTCAAGAAGTTTCCACACAT+CGG + Chr6:108477970-108477989 None:intergenic 40.0%
TAAGGTTGAAGCTCCCATTA+AGG - Chr6:108477887-108477906 MsG0680035562.01.T01:CDS 40.0%
TAGAAATGCTGTGTTGGAGT+TGG - Chr6:108478119-108478138 MsG0680035562.01.T01:CDS 40.0%
TGAAGCTTCCGTTAAAGTTG+AGG - Chr6:108477929-108477948 MsG0680035562.01.T01:CDS 40.0%
TGAAGGTAGAAATGCTGTGT+TGG - Chr6:108478113-108478132 MsG0680035562.01.T01:CDS 40.0%
!! TGTTGGAGTTGGTTTGTGAA+CGG - Chr6:108478130-108478149 MsG0680035562.01.T01:CDS 40.0%
AACGGAAGCTTCAACCTTCA+CGG + Chr6:108477922-108477941 None:intergenic 45.0%
ACGGAAGCTTCGACCTTAAT+GGG + Chr6:108477903-108477922 None:intergenic 45.0%
AGAAGGTTCCCTGCTACATA+TGG + Chr6:108478312-108478331 None:intergenic 45.0%
CCATTAATCTTCCGGCAAGA+AGG + Chr6:108478329-108478348 None:intergenic 45.0%
CCTTCTTGCCGGAAGATTAA+TGG - Chr6:108478326-108478345 MsG0680035562.01.T01:CDS 45.0%
CTACATATGGCACCATCTCA+TGG + Chr6:108478299-108478318 None:intergenic 45.0%
GCAACGGGATCAAGAAAATG+TGG - Chr6:108478195-108478214 MsG0680035562.01.T01:intron 45.0%
GGTTCGTCGACAAGCAAATA+GGG - Chr6:108478059-108478078 MsG0680035562.01.T01:CDS 45.0%
GTCGTCCTCCATTAATCTTC+CGG + Chr6:108478337-108478356 None:intergenic 45.0%
TCTTGCCGGAAGATTAATGG+AGG - Chr6:108478329-108478348 MsG0680035562.01.T01:CDS 45.0%
!! AGATGGTGCCATATGTAGCA+GGG - Chr6:108478301-108478320 MsG0680035562.01.T01:intron 45.0%
ACACTTGGGTTTCGATCACC+CGG + Chr6:108477855-108477874 None:intergenic 50.0%
CAAGCGTCAATTCCGATGTG+TGG - Chr6:108477955-108477974 MsG0680035562.01.T01:CDS 50.0%
CACGGAAGCTTCGACCTTAA+TGG + Chr6:108477904-108477923 None:intergenic 50.0%
GGGTTCGTCGACAAGCAAAT+AGG - Chr6:108478058-108478077 MsG0680035562.01.T01:CDS 50.0%
TAAGGTCGAAGCTTCCGTGA+AGG - Chr6:108477905-108477924 MsG0680035562.01.T01:CDS 50.0%
TCGTCAGGGAAGACAGTTCT+TGG - Chr6:108478244-108478263 MsG0680035562.01.T01:intron 50.0%
TGAGAATGCGGGTGATGATC+CGG - Chr6:108477833-108477852 MsG0680035562.01.T01:CDS 50.0%
TGGCGTTCACAACCATGAGA+TGG - Chr6:108478284-108478303 MsG0680035562.01.T01:intron 50.0%
!! GAGATGGTGCCATATGTAGC+AGG - Chr6:108478300-108478319 MsG0680035562.01.T01:intron 50.0%
!! GAGTTGGTTTGTGAACGGAG+TGG - Chr6:108478135-108478154 MsG0680035562.01.T01:CDS 50.0%
AGGTGCGTGGATATGTCGTC+AGG - Chr6:108478229-108478248 MsG0680035562.01.T01:intron 55.0%
GAGAATGCGGGTGATGATCC+GGG - Chr6:108477834-108477853 MsG0680035562.01.T01:CDS 55.0%
GGGTGTTTGTTCAAGGTGCG+TGG - Chr6:108478216-108478235 MsG0680035562.01.T01:intron 55.0%
GGTGCGTGGATATGTCGTCA+GGG - Chr6:108478230-108478249 MsG0680035562.01.T01:intron 55.0%
GTAGCAGGGAACCTTCTTGC+CGG - Chr6:108478315-108478334 MsG0680035562.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 108477828 108478467 108477828 ID=MsG0680035562.01;Name=MsG0680035562.01
Chr6 mRNA 108477828 108478467 108477828 ID=MsG0680035562.01.T01;Parent=MsG0680035562.01;Name=MsG0680035562.01.T01;_AED=0.58;_eAED=0.58;_QI=0|0|0|0.5|1|1|2|0|163
Chr6 exon 108478322 108478467 108478322 ID=MsG0680035562.01.T01:exon:17403;Parent=MsG0680035562.01.T01
Chr6 exon 108477828 108478173 108477828 ID=MsG0680035562.01.T01:exon:17402;Parent=MsG0680035562.01.T01
Chr6 CDS 108478322 108478467 108478322 ID=MsG0680035562.01.T01:cds;Parent=MsG0680035562.01.T01
Chr6 CDS 108477828 108478173 108477828 ID=MsG0680035562.01.T01:cds;Parent=MsG0680035562.01.T01
Gene Sequence

>MsG0680035562.01.T01

ATGGTTGAGAATGCGGGTGATGATCCGGGTGATCGAAACCCAAGTGTTGTCGATTCAGTTAAGGTTGAAGCTCCCATTAAGGTCGAAGCTTCCGTGAAGGTTGAAGCTTCCGTTAAAGTTGAGGCTTCAAGCGTCAATTCCGATGTAAATGCTGTGTTGGAGTTGGTTTGTGAACGGAGTGGCGAGCACAAAGTACCGAAGACAAAAGTGAAGCATGAAGCAACGGGATCAAGAAAATGTGGGTGTTTGTTCAAGGTGCGTGGATATGTCGTCAGGGAAGACAGTTCTTGGAAATTGGCTATTCTTAATGGCGTTCACAACCATGAGATGGTGCCATATGTAGCAGGGAACCTTCTTGCCGGAAGATTAATGGAGGACGACAAGAAGATTGTACATGACTTGACCAATTCTTTGGTGAAACCAAAAAATATTGAAAAATTTGAAGAAGAAAAGGAAAGAGTCCATGACAAATATCAAGCAAGTCTACAATGA

Protein sequence

>MsG0680035562.01.T01

MVENAGDDPGDRNPSVVDSVKVEAPIKVEASVKVEASVKVEASSVNSDVNAVLELVCERSGEHKVPKTKVKHEATGSRKCGCLFKVRGYVVREDSSWKLAILNGVHNHEMVPYVAGNLLAGRLMEDDKKIVHDLTNSLVKPKNIEKFEEEKERVHDKYQASLQ*