Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036528.01.T01 | XP_024626129.1 | 82.784 | 273 | 47 | 0 | 1 | 273 | 1 | 273 | 6.14E-153 | 437 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036528.01.T01 | Q9LFQ3 | 38.608 | 158 | 93 | 3 | 93 | 250 | 46 | 199 | 1.23E-20 | 95.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036528.01.T01 | A0A396GZV2 | 82.784 | 273 | 47 | 0 | 1 | 273 | 1 | 273 | 2.93e-153 | 437 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780036528.01.T01 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036528.01.T01 | MTR_7g013380 | 82.824 | 262 | 45 | 0 | 1 | 262 | 1 | 262 | 3.67e-150 | 422 |
| MsG0780036528.01.T01 | MTR_4g133300 | 34.188 | 234 | 145 | 4 | 26 | 259 | 20 | 244 | 7.58e-34 | 123 |
| MsG0780036528.01.T01 | MTR_4g133300 | 35.673 | 171 | 101 | 5 | 5 | 173 | 81 | 244 | 9.24e-20 | 86.3 |
| MsG0780036528.01.T01 | MTR_4g133470 | 33.761 | 234 | 146 | 4 | 26 | 259 | 20 | 244 | 2.14e-32 | 119 |
| MsG0780036528.01.T01 | MTR_4g133470 | 36.257 | 171 | 100 | 5 | 5 | 173 | 81 | 244 | 2.36e-19 | 85.1 |
| MsG0780036528.01.T01 | MTR_3g006125 | 39.521 | 167 | 93 | 3 | 99 | 259 | 89 | 253 | 3.13e-26 | 103 |
| MsG0780036528.01.T01 | MTR_3g006125 | 38.415 | 164 | 93 | 3 | 11 | 168 | 87 | 248 | 2.30e-23 | 96.3 |
| MsG0780036528.01.T01 | MTR_1g105955 | 43.796 | 137 | 70 | 2 | 118 | 254 | 33 | 162 | 9.48e-26 | 100 |
| MsG0780036528.01.T01 | MTR_1g105955 | 37.821 | 156 | 86 | 4 | 14 | 168 | 17 | 162 | 7.55e-20 | 84.7 |
| MsG0780036528.01.T01 | MTR_3g006185 | 37.952 | 166 | 96 | 3 | 99 | 259 | 8 | 171 | 5.92e-24 | 95.5 |
| MsG0780036528.01.T01 | MTR_3g006185 | 36.471 | 170 | 101 | 5 | 9 | 173 | 4 | 171 | 3.99e-20 | 85.5 |
| MsG0780036528.01.T01 | MTR_3g006105 | 38.554 | 166 | 93 | 4 | 99 | 258 | 8 | 170 | 1.15e-23 | 95.1 |
| MsG0780036528.01.T01 | MTR_3g006105 | 35.366 | 164 | 98 | 3 | 11 | 168 | 6 | 167 | 2.41e-19 | 83.6 |
| MsG0780036528.01.T01 | MTR_3g006105 | 45.000 | 80 | 43 | 1 | 186 | 265 | 9 | 87 | 3.85e-13 | 66.6 |
| MsG0780036528.01.T01 | MTR_8g062700 | 41.304 | 138 | 77 | 3 | 115 | 250 | 56 | 191 | 2.12e-23 | 100 |
| MsG0780036528.01.T01 | MTR_8g062700 | 37.681 | 138 | 82 | 3 | 29 | 164 | 56 | 191 | 3.38e-18 | 84.7 |
| MsG0780036528.01.T01 | MTR_8g062700 | 40.860 | 93 | 54 | 1 | 171 | 262 | 25 | 117 | 3.59e-14 | 72.8 |
| MsG0780036528.01.T01 | MTR_8g062700 | 41.304 | 138 | 77 | 3 | 115 | 250 | 56 | 191 | 2.22e-23 | 100 |
| MsG0780036528.01.T01 | MTR_8g062700 | 37.681 | 138 | 82 | 3 | 29 | 164 | 56 | 191 | 3.58e-18 | 84.7 |
| MsG0780036528.01.T01 | MTR_8g062700 | 40.860 | 93 | 54 | 1 | 171 | 262 | 25 | 117 | 3.79e-14 | 72.8 |
| MsG0780036528.01.T01 | MTR_3g006065 | 38.411 | 151 | 89 | 3 | 105 | 252 | 7 | 156 | 4.97e-23 | 93.6 |
| MsG0780036528.01.T01 | MTR_3g006065 | 38.849 | 139 | 77 | 3 | 32 | 166 | 22 | 156 | 3.10e-19 | 83.6 |
| MsG0780036528.01.T01 | MTR_3g006150 | 37.419 | 155 | 93 | 3 | 101 | 252 | 3 | 156 | 6.75e-23 | 92.4 |
| MsG0780036528.01.T01 | MTR_3g006150 | 39.568 | 139 | 76 | 3 | 32 | 166 | 22 | 156 | 4.20e-21 | 87.8 |
| MsG0780036528.01.T01 | MTR_1g104960 | 34.356 | 163 | 101 | 3 | 99 | 259 | 13 | 171 | 1.58e-22 | 92.0 |
| MsG0780036528.01.T01 | MTR_1g104960 | 30.864 | 162 | 106 | 3 | 11 | 170 | 11 | 168 | 4.57e-17 | 77.4 |
| MsG0780036528.01.T01 | MTR_1g104980 | 33.742 | 163 | 87 | 4 | 102 | 262 | 2 | 145 | 1.09e-20 | 87.0 |
| MsG0780036528.01.T01 | MTR_1g104980 | 33.951 | 162 | 90 | 3 | 16 | 177 | 2 | 146 | 1.29e-19 | 84.0 |
| MsG0780036528.01.T01 | MTR_5g036670 | 36.620 | 142 | 80 | 3 | 115 | 251 | 57 | 193 | 1.83e-19 | 88.6 |
| MsG0780036528.01.T01 | MTR_5g036670 | 34.752 | 141 | 84 | 3 | 29 | 165 | 57 | 193 | 1.01e-15 | 77.4 |
| MsG0780036528.01.T01 | MTR_5g036670 | 46.970 | 66 | 35 | 0 | 201 | 266 | 57 | 122 | 6.00e-12 | 66.2 |
| MsG0780036528.01.T01 | MTR_7g013410 | 32.653 | 147 | 92 | 3 | 103 | 249 | 2 | 141 | 6.58e-19 | 81.3 |
| MsG0780036528.01.T01 | MTR_7g013410 | 31.973 | 147 | 93 | 3 | 17 | 163 | 2 | 141 | 1.94e-18 | 80.1 |
| MsG0780036528.01.T01 | MTR_6g008970 | 34.177 | 158 | 98 | 3 | 95 | 250 | 59 | 212 | 1.94e-18 | 85.5 |
| MsG0780036528.01.T01 | MTR_6g008970 | 31.373 | 153 | 99 | 3 | 14 | 164 | 64 | 212 | 8.73e-14 | 71.6 |
| MsG0780036528.01.T01 | MTR_6g008995 | 33.553 | 152 | 97 | 2 | 99 | 250 | 37 | 184 | 4.82e-18 | 84.0 |
| MsG0780036528.01.T01 | MTR_6g008995 | 30.464 | 151 | 101 | 2 | 14 | 164 | 38 | 184 | 4.46e-14 | 72.0 |
| MsG0780036528.01.T01 | MTR_6g008995 | 33.594 | 128 | 83 | 1 | 123 | 250 | 2 | 127 | 8.47e-15 | 73.9 |
| MsG0780036528.01.T01 | MTR_6g008995 | 30.469 | 128 | 87 | 1 | 37 | 164 | 2 | 127 | 6.04e-12 | 65.5 |
| MsG0780036528.01.T01 | MTR_1g104940 | 43.750 | 80 | 43 | 1 | 183 | 262 | 12 | 89 | 4.32e-13 | 65.1 |
| MsG0780036528.01.T01 | MTR_1g104940 | 41.237 | 97 | 51 | 2 | 100 | 192 | 15 | 109 | 6.95e-13 | 64.3 |
| MsG0780036528.01.T01 | MTR_1g104937 | 45.455 | 77 | 40 | 1 | 186 | 262 | 13 | 87 | 7.50e-13 | 63.9 |
| MsG0780036528.01.T01 | MTR_1g104937 | 44.156 | 77 | 41 | 1 | 100 | 176 | 13 | 87 | 8.91e-13 | 63.9 |
| MsG0780036528.01.T01 | MTR_1g104937 | 44.444 | 81 | 42 | 2 | 11 | 90 | 10 | 88 | 3.18e-11 | 59.7 |
| MsG0780036528.01.T01 | MTR_1g105960 | 33.945 | 109 | 46 | 1 | 115 | 223 | 15 | 97 | 3.19e-12 | 62.4 |
| MsG0780036528.01.T01 | MTR_1g105960 | 43.548 | 62 | 35 | 0 | 201 | 262 | 15 | 76 | 6.18e-11 | 58.5 |
| MsG0780036528.01.T01 | MTR_1g105010 | 38.202 | 89 | 51 | 2 | 95 | 183 | 12 | 96 | 5.20e-11 | 59.3 |
| MsG0780036528.01.T01 | MTR_1g105010 | 42.857 | 77 | 42 | 1 | 186 | 262 | 17 | 91 | 6.30e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036528.01.T01 | AT5G15020 | 38.608 | 158 | 93 | 3 | 93 | 250 | 46 | 199 | 1.25e-21 | 95.1 |
| MsG0780036528.01.T01 | AT5G15020 | 35.294 | 153 | 95 | 3 | 14 | 166 | 53 | 201 | 2.48e-16 | 79.3 |
| MsG0780036528.01.T01 | AT5G15020 | 38.608 | 158 | 93 | 3 | 93 | 250 | 46 | 199 | 1.39e-21 | 94.7 |
| MsG0780036528.01.T01 | AT5G15020 | 35.294 | 153 | 95 | 3 | 14 | 166 | 53 | 201 | 2.57e-16 | 79.3 |
| MsG0780036528.01.T01 | AT1G24190 | 37.654 | 162 | 95 | 5 | 91 | 250 | 6 | 163 | 2.12e-21 | 94.4 |
| MsG0780036528.01.T01 | AT1G24190 | 34.104 | 173 | 107 | 6 | 3 | 172 | 4 | 172 | 2.17e-17 | 82.4 |
| MsG0780036528.01.T01 | AT1G24190 | 41.975 | 81 | 45 | 1 | 186 | 266 | 15 | 93 | 7.01e-11 | 63.2 |
| MsG0780036528.01.T01 | AT1G24190 | 37.654 | 162 | 95 | 5 | 91 | 250 | 6 | 163 | 2.12e-21 | 94.4 |
| MsG0780036528.01.T01 | AT1G24190 | 34.104 | 173 | 107 | 6 | 3 | 172 | 4 | 172 | 1.96e-17 | 82.4 |
| MsG0780036528.01.T01 | AT1G24190 | 41.975 | 81 | 45 | 1 | 186 | 266 | 15 | 93 | 6.71e-11 | 63.2 |
| MsG0780036528.01.T01 | AT1G24190 | 37.654 | 162 | 95 | 5 | 91 | 250 | 6 | 163 | 2.14e-21 | 94.4 |
| MsG0780036528.01.T01 | AT1G24190 | 34.104 | 173 | 107 | 6 | 3 | 172 | 4 | 172 | 1.99e-17 | 82.4 |
| MsG0780036528.01.T01 | AT1G24190 | 45.455 | 66 | 36 | 0 | 201 | 266 | 28 | 93 | 7.14e-11 | 63.2 |
| MsG0780036528.01.T01 | AT1G70030 | 35.032 | 157 | 92 | 4 | 96 | 249 | 8 | 157 | 1.49e-20 | 86.3 |
| MsG0780036528.01.T01 | AT1G70030 | 35.099 | 151 | 92 | 3 | 14 | 163 | 12 | 157 | 1.60e-18 | 80.9 |
| MsG0780036528.01.T01 | AT1G70060 | 34.756 | 164 | 100 | 4 | 90 | 250 | 5 | 164 | 1.21e-19 | 89.0 |
| MsG0780036528.01.T01 | AT1G70060 | 32.727 | 165 | 104 | 4 | 3 | 164 | 4 | 164 | 1.32e-16 | 80.1 |
| MsG0780036528.01.T01 | AT1G70060 | 34.756 | 164 | 100 | 4 | 90 | 250 | 5 | 164 | 1.21e-19 | 89.0 |
| MsG0780036528.01.T01 | AT1G70060 | 32.727 | 165 | 104 | 4 | 3 | 164 | 4 | 164 | 1.32e-16 | 80.1 |
| MsG0780036528.01.T01 | AT3G01320 | 30.918 | 207 | 135 | 4 | 47 | 249 | 2 | 204 | 1.33e-18 | 85.9 |
| MsG0780036528.01.T01 | AT3G01320 | 29.464 | 224 | 145 | 6 | 14 | 232 | 58 | 273 | 5.30e-17 | 81.3 |
| MsG0780036528.01.T01 | AT3G01320 | 46.970 | 66 | 35 | 0 | 201 | 266 | 71 | 136 | 2.43e-11 | 64.3 |
| MsG0780036528.01.T01 | AT3G01320 | 30.918 | 207 | 135 | 4 | 47 | 249 | 2 | 204 | 1.39e-18 | 85.9 |
| MsG0780036528.01.T01 | AT3G01320 | 29.464 | 224 | 145 | 6 | 14 | 232 | 58 | 273 | 5.34e-17 | 81.3 |
| MsG0780036528.01.T01 | AT3G01320 | 46.970 | 66 | 35 | 0 | 201 | 266 | 71 | 136 | 2.51e-11 | 64.3 |
| MsG0780036528.01.T01 | AT1G59890 | 33.824 | 136 | 88 | 1 | 115 | 250 | 59 | 192 | 5.73e-18 | 84.0 |
| MsG0780036528.01.T01 | AT1G59890 | 32.836 | 134 | 88 | 1 | 31 | 164 | 61 | 192 | 3.48e-15 | 75.9 |
| MsG0780036528.01.T01 | AT1G59890 | 33.824 | 136 | 88 | 1 | 115 | 250 | 59 | 192 | 6.30e-18 | 84.0 |
| MsG0780036528.01.T01 | AT1G59890 | 32.836 | 134 | 88 | 1 | 31 | 164 | 61 | 192 | 3.97e-15 | 75.9 |
| MsG0780036528.01.T01 | AT1G59890 | 33.824 | 136 | 88 | 1 | 115 | 250 | 59 | 192 | 6.49e-18 | 84.0 |
| MsG0780036528.01.T01 | AT1G59890 | 32.836 | 134 | 88 | 1 | 31 | 164 | 61 | 192 | 4.01e-15 | 75.9 |
| MsG0780036528.01.T01 | AT1G59890 | 33.824 | 136 | 88 | 1 | 115 | 250 | 59 | 192 | 6.61e-18 | 84.0 |
| MsG0780036528.01.T01 | AT1G59890 | 32.836 | 134 | 88 | 1 | 31 | 164 | 61 | 192 | 4.01e-15 | 75.9 |
| MsG0780036528.01.T01 | AT1G10450 | 33.813 | 139 | 90 | 1 | 115 | 253 | 98 | 234 | 4.74e-17 | 81.3 |
| MsG0780036528.01.T01 | AT1G10450 | 31.410 | 156 | 104 | 2 | 31 | 186 | 100 | 252 | 3.87e-15 | 75.9 |
| MsG0780036528.01.T01 | AT1G10450 | 33.813 | 139 | 90 | 1 | 115 | 253 | 47 | 183 | 6.40e-17 | 80.9 |
| MsG0780036528.01.T01 | AT1G10450 | 31.410 | 156 | 104 | 2 | 31 | 186 | 49 | 201 | 4.75e-15 | 75.5 |
| MsG0780036528.01.T01 | AT1G10450 | 33.813 | 139 | 90 | 1 | 115 | 253 | 47 | 183 | 6.40e-17 | 80.9 |
| MsG0780036528.01.T01 | AT1G10450 | 31.410 | 156 | 104 | 2 | 31 | 186 | 49 | 201 | 4.75e-15 | 75.5 |
| MsG0780036528.01.T01 | AT1G24230 | 24.590 | 244 | 155 | 6 | 3 | 241 | 4 | 223 | 7.86e-17 | 78.2 |
| MsG0780036528.01.T01 | AT1G24230 | 27.545 | 167 | 105 | 4 | 95 | 261 | 10 | 160 | 5.22e-13 | 67.4 |
| MsG0780036528.01.T01 | AT1G24200 | 31.169 | 154 | 101 | 4 | 96 | 249 | 11 | 159 | 2.76e-14 | 70.1 |
| MsG0780036528.01.T01 | AT1G70030 | 28.662 | 157 | 70 | 4 | 96 | 249 | 19 | 136 | 1.65e-11 | 61.2 |
| MsG0780036528.01.T01 | AT1G70030 | 35.577 | 104 | 60 | 3 | 95 | 195 | 18 | 117 | 4.90e-11 | 59.3 |
| MsG0780036528.01.T01 | AT5G35610 | 42.647 | 68 | 39 | 0 | 201 | 268 | 24 | 91 | 9.76e-11 | 59.3 |
Find 47 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACTTTCTGAATGTCTTTA+AGG | 0.070134 | 7:-9359788 | MsG0780036528.01.T01:CDS |
| GGAACAATTTCACGTTAAAT+TGG | 0.277062 | 7:-9359142 | MsG0780036528.01.T01:CDS |
| ATAGATTTATTCAAGTTAAC+AGG | 0.306329 | 7:-9360189 | MsG0780036528.01.T01:CDS |
| AACATCATTGACAAATTCTT+TGG | 0.313079 | 7:+9359496 | None:intergenic |
| TAGAATTGATGATGCAAATC+TGG | 0.365588 | 7:-9359265 | MsG0780036528.01.T01:CDS |
| AGAACATTGTGACGTCAAAA+AGG | 0.380889 | 7:-9360239 | MsG0780036528.01.T01:CDS |
| ATCTGAGGAGGAACAATCTC+TGG | 0.416325 | 7:-9359896 | MsG0780036528.01.T01:CDS |
| AGTAGTTCAATCTTGATATA+AGG | 0.418112 | 7:+9359594 | None:intergenic |
| TAACTTTCTGAATGTGTTGA+AGG | 0.435901 | 7:-9359432 | MsG0780036528.01.T01:CDS |
| AAGGGATTATTTGAAGGTCA+TGG | 0.456386 | 7:-9359976 | MsG0780036528.01.T01:CDS |
| GAATCTTGGTGAATTATTGA+AGG | 0.480427 | 7:-9359639 | MsG0780036528.01.T01:CDS |
| AGGATTGACGCTGCGAATCT+TGG | 0.486810 | 7:-9359653 | MsG0780036528.01.T01:CDS |
| AGCTTGATTTCAAATCCATC+AGG | 0.489510 | 7:+9359922 | None:intergenic |
| AAATCTCAGTAAATTCTTGA+AGG | 0.490603 | 7:-9359995 | MsG0780036528.01.T01:CDS |
| TTCTTGAAGGGATTATTTGA+AGG | 0.491097 | 7:-9359982 | MsG0780036528.01.T01:CDS |
| AGCTTGATTTCATATCCATC+AGG | 0.493606 | 7:+9359177 | None:intergenic |
| CAGCTTGATTTCAAATCCAT+CGG | 0.495338 | 7:+9359565 | None:intergenic |
| AACTTCATTGACGAACTCCT+TGG | 0.507296 | 7:+9360216 | None:intergenic |
| AGCTTGATTTCAAATCCATC+GGG | 0.513708 | 7:+9359566 | None:intergenic |
| TTATTGAAGGGATTATTTCA+AGG | 0.513778 | 7:-9359626 | MsG0780036528.01.T01:CDS |
| ATAATGAAGAAAATATTTGA+AGG | 0.513952 | 7:-9359237 | MsG0780036528.01.T01:CDS |
| AATCTTGGTGAATTATTGAA+GGG | 0.514858 | 7:-9359638 | MsG0780036528.01.T01:CDS |
| TTGAACTACTTCATGCCTGA+TGG | 0.515459 | 7:-9359937 | MsG0780036528.01.T01:CDS |
| AATCTCAGTAAATTCTTGAA+GGG | 0.520509 | 7:-9359994 | MsG0780036528.01.T01:CDS |
| AATCAAGCTGCCATCGGAGG+AGG | 0.529195 | 7:-9359552 | MsG0780036528.01.T01:CDS |
| TGAAATCAAGCTTCCATTGG+AGG | 0.531076 | 7:-9359166 | MsG0780036528.01.T01:CDS |
| TTAAACTACTTCCTACCTGA+TGG | 0.558050 | 7:-9359192 | MsG0780036528.01.T01:CDS |
| AGAGATTCTTCCTCCTCCGA+TGG | 0.570247 | 7:+9359542 | None:intergenic |
| TGAAATTGTTCCTCCTCCAA+TGG | 0.571267 | 7:+9359153 | None:intergenic |
| ATATGAAATCAAGCTTCCAT+TGG | 0.577817 | 7:-9359169 | MsG0780036528.01.T01:CDS |
| TTGAACTACTTCATGCCCGA+TGG | 0.579628 | 7:-9359581 | MsG0780036528.01.T01:CDS |
| ATTTATTCAAGTTAATCAGA+GGG | 0.582516 | 7:-9359465 | MsG0780036528.01.T01:CDS |
| TGATGCATTACAAGAAACAA+AGG | 0.583155 | 7:-9360135 | MsG0780036528.01.T01:intron |
| GATTTATTCAAGTTAATCAG+AGG | 0.587296 | 7:-9359466 | MsG0780036528.01.T01:CDS |
| GATTTATTCAAGTTAATCAG+AGG | 0.587296 | 7:-9359822 | MsG0780036528.01.T01:CDS |
| TGAAATCAAGCTGCCATCGG+AGG | 0.588675 | 7:-9359555 | MsG0780036528.01.T01:CDS |
| TGAAATCAAGCTTCTATCTG+AGG | 0.589635 | 7:-9359911 | MsG0780036528.01.T01:CDS |
| ATTTATTCAAGTTAATCAGA+GGG | 0.591574 | 7:-9359821 | MsG0780036528.01.T01:CDS |
| GATTTATTCAAGTTAACAGG+AGG | 0.594070 | 7:-9360186 | MsG0780036528.01.T01:CDS |
| TGAAGGATTACAAGAGACAA+AGG | 0.594478 | 7:-9359415 | MsG0780036528.01.T01:intron |
| AATCAAGCTTCCATTGGAGG+AGG | 0.611644 | 7:-9359163 | MsG0780036528.01.T01:CDS |
| GGGCAACCCGTGATAATGGA+AGG | 0.616033 | 7:-9360271 | None:intergenic |
| ATTTATTCAAGTTAACAGGA+GGG | 0.617938 | 7:-9360185 | MsG0780036528.01.T01:CDS |
| ATTTGAAATCAAGCTGCCAT+CGG | 0.624832 | 7:-9359558 | MsG0780036528.01.T01:CDS |
| TGATTTCATATCCATCAGGT+AGG | 0.626891 | 7:+9359181 | None:intergenic |
| AATCAAGCTTCTATCTGAGG+AGG | 0.631110 | 7:-9359908 | MsG0780036528.01.T01:CDS |
| TTAAGGATTACAAAACACAA+AGG | 0.659829 | 7:-9359771 | MsG0780036528.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATATAATAAAAAAATAG+TGG | + | Chr7:9359648-9359667 | None:intergenic | 10.0% |
| !!! | TTTTTTATTATATTGTGAAA+TGG | - | Chr7:9359652-9359671 | MsG0780036528.01.T01:CDS | 10.0% |
| !! | ATAATGAAGAAAATATTTGA+AGG | - | Chr7:9360141-9360160 | MsG0780036528.01.T01:CDS | 15.0% |
| !! | CATTACAATAAAAAAAATAG+TGG | + | Chr7:9359284-9359303 | None:intergenic | 15.0% |
| !! | GCAATATAATAAAAAAATAG+TGG | + | Chr7:9360004-9360023 | None:intergenic | 15.0% |
| !!! | TTTTTTATTATATTGCGAAA+TGG | - | Chr7:9360008-9360027 | MsG0780036528.01.T01:CDS | 15.0% |
| !! | ATTCTACAAAATATACCAAA+TGG | + | Chr7:9360099-9360118 | None:intergenic | 20.0% |
| !!! | AACTAATTTATCTATCCTTT+TGG | - | Chr7:9359336-9359355 | MsG0780036528.01.T01:intron | 20.0% |
| !!! | ATAGATTTATTCAAGTTAAC+AGG | - | Chr7:9359189-9359208 | MsG0780036528.01.T01:CDS | 20.0% |
| !!! | ATTTATTCAAGTTAATCAGA+GGG | - | Chr7:9359557-9359576 | MsG0780036528.01.T01:CDS | 20.0% |
| !!! | TATTAATTTGTCTATCCATT+TGG | - | Chr7:9360081-9360100 | MsG0780036528.01.T01:intron | 20.0% |
| !!! | TGGATTCTATTAATTTTTGT+TGG | - | Chr7:9360054-9360073 | MsG0780036528.01.T01:intron | 20.0% |
| ! | AAATCTCAGTAAATTCTTGA+AGG | - | Chr7:9359383-9359402 | MsG0780036528.01.T01:intron | 25.0% |
| ! | AACATCATTGACAAATTCTT+TGG | + | Chr7:9359529-9359548 | None:intergenic | 25.0% |
| ! | AATCTCAGTAAATTCTTGAA+GGG | - | Chr7:9359384-9359403 | MsG0780036528.01.T01:intron | 25.0% |
| ! | AATCTTGGTGAATTATTGAA+GGG | - | Chr7:9359740-9359759 | MsG0780036528.01.T01:intron | 25.0% |
| ! | AGTAGTTCAATCTTGATATA+AGG | + | Chr7:9359431-9359450 | None:intergenic | 25.0% |
| ! | ATCCTACAAAATATACCAAA+AGG | + | Chr7:9359354-9359373 | None:intergenic | 25.0% |
| ! | TAACTTTCTGAATGTCTTTA+AGG | - | Chr7:9359590-9359609 | MsG0780036528.01.T01:CDS | 25.0% |
| ! | TTAAGGATTACAAAACACAA+AGG | - | Chr7:9359607-9359626 | MsG0780036528.01.T01:CDS | 25.0% |
| ! | TTATTGAAGGGATTATTTCA+AGG | - | Chr7:9359752-9359771 | MsG0780036528.01.T01:intron | 25.0% |
| !! | ATCCTTTTGGTATATTTTGT+AGG | - | Chr7:9359349-9359368 | MsG0780036528.01.T01:intron | 25.0% |
| !! | ATTTATTCAAGTTAACAGGA+GGG | - | Chr7:9359193-9359212 | MsG0780036528.01.T01:CDS | 25.0% |
| !! | GATTTATTCAAGTTAATCAG+AGG | - | Chr7:9359556-9359575 | MsG0780036528.01.T01:CDS | 25.0% |
| ATATGAAATCAAGCTTCCAT+TGG | - | Chr7:9360209-9360228 | MsG0780036528.01.T01:CDS | 30.0% | |
| GAATCTTGGTGAATTATTGA+AGG | - | Chr7:9359739-9359758 | MsG0780036528.01.T01:intron | 30.0% | |
| GGAACAATTTCACGTTAAAT+TGG | - | Chr7:9360236-9360255 | MsG0780036528.01.T01:CDS | 30.0% | |
| TAACTTTCTGAATGTGTTGA+AGG | - | Chr7:9359946-9359965 | MsG0780036528.01.T01:CDS | 30.0% | |
| TGATGCATTACAAGAAACAA+AGG | - | Chr7:9359243-9359262 | MsG0780036528.01.T01:CDS | 30.0% | |
| TTCTTGAAGGGATTATTTGA+AGG | - | Chr7:9359396-9359415 | MsG0780036528.01.T01:intron | 30.0% | |
| ! | GACAAATTCTTTCGCATTTT+TGG | + | Chr7:9359520-9359539 | None:intergenic | 30.0% |
| ! | GATTTATTCAAGTTAACAGG+AGG | - | Chr7:9359192-9359211 | MsG0780036528.01.T01:CDS | 30.0% |
| !! | TAGAATTGATGATGCAAATC+TGG | - | Chr7:9360113-9360132 | MsG0780036528.01.T01:intron | 30.0% |
| AAGGGATTATTTGAAGGTCA+TGG | - | Chr7:9359402-9359421 | MsG0780036528.01.T01:intron | 35.0% | |
| AGAACATTGTGACGTCAAAA+AGG | - | Chr7:9359139-9359158 | MsG0780036528.01.T01:CDS | 35.0% | |
| AGCTTGATTTCAAATCCATC+AGG | + | Chr7:9359459-9359478 | None:intergenic | 35.0% | |
| AGCTTGATTTCAAATCCATC+GGG | + | Chr7:9359459-9359478 | None:intergenic | 35.0% | |
| AGCTTGATTTCATATCCATC+AGG | + | Chr7:9360204-9360223 | None:intergenic | 35.0% | |
| CAGCTTGATTTCAAATCCAT+CGG | + | Chr7:9359816-9359835 | None:intergenic | 35.0% | |
| TGAAATCAAGCTTCTATCTG+AGG | - | Chr7:9359467-9359486 | MsG0780036528.01.T01:CDS | 35.0% | |
| TGAAGGATTACAAGAGACAA+AGG | - | Chr7:9359963-9359982 | MsG0780036528.01.T01:CDS | 35.0% | |
| TGATTTCATATCCATCAGGT+AGG | + | Chr7:9360200-9360219 | None:intergenic | 35.0% | |
| TTAAACTACTTCCTACCTGA+TGG | - | Chr7:9360186-9360205 | MsG0780036528.01.T01:CDS | 35.0% | |
| ! | ATTTGAAATCAAGCTGCCAT+CGG | - | Chr7:9359820-9359839 | MsG0780036528.01.T01:CDS | 35.0% |
| AACTTCATTGACGAACTCCT+TGG | + | Chr7:9359165-9359184 | None:intergenic | 40.0% | |
| AATCAAGCTTCTATCTGAGG+AGG | - | Chr7:9359470-9359489 | MsG0780036528.01.T01:CDS | 40.0% | |
| TGAAATCAAGCTTCCATTGG+AGG | - | Chr7:9360212-9360231 | MsG0780036528.01.T01:CDS | 40.0% | |
| TGAAATTGTTCCTCCTCCAA+TGG | + | Chr7:9360228-9360247 | None:intergenic | 40.0% | |
| TTGAACTACTTCATGCCTGA+TGG | - | Chr7:9359441-9359460 | MsG0780036528.01.T01:CDS | 40.0% | |
| AATCAAGCTTCCATTGGAGG+AGG | - | Chr7:9360215-9360234 | MsG0780036528.01.T01:CDS | 45.0% | |
| ATCTGAGGAGGAACAATCTC+TGG | - | Chr7:9359482-9359501 | MsG0780036528.01.T01:CDS | 45.0% | |
| TTGAACTACTTCATGCCCGA+TGG | - | Chr7:9359797-9359816 | MsG0780036528.01.T01:CDS | 45.0% | |
| TTGTGACGTCAAAAAGGCCA+AGG | - | Chr7:9359145-9359164 | MsG0780036528.01.T01:CDS | 45.0% | |
| !!! | ATTTTTTTATTATATTTTGT+AGG | - | Chr7:9359705-9359724 | MsG0780036528.01.T01:intron | 5.0% |
| !!! | TTTATTTTTAAATAATGAAA+TGG | - | Chr7:9360034-9360053 | MsG0780036528.01.T01:intron | 5.0% |
| AGAGATTCTTCCTCCTCCGA+TGG | + | Chr7:9359839-9359858 | None:intergenic | 50.0% | |
| AGGATTGACGCTGCGAATCT+TGG | - | Chr7:9359725-9359744 | MsG0780036528.01.T01:intron | 50.0% | |
| TGAAATCAAGCTGCCATCGG+AGG | - | Chr7:9359823-9359842 | MsG0780036528.01.T01:CDS | 50.0% | |
| AATCAAGCTGCCATCGGAGG+AGG | - | Chr7:9359826-9359845 | MsG0780036528.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 9359122 | 9360278 | 9359122 | ID=MsG0780036528.01;Name=MsG0780036528.01 |
| Chr7 | mRNA | 9359122 | 9360278 | 9359122 | ID=MsG0780036528.01.T01;Parent=MsG0780036528.01;Name=MsG0780036528.01.T01;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|0.75|4|0|273 |
| Chr7 | exon | 9360136 | 9360278 | 9360136 | ID=MsG0780036528.01.T01:exon:10290;Parent=MsG0780036528.01.T01 |
| Chr7 | exon | 9359772 | 9360029 | 9359772 | ID=MsG0780036528.01.T01:exon:10289;Parent=MsG0780036528.01.T01 |
| Chr7 | exon | 9359416 | 9359673 | 9359416 | ID=MsG0780036528.01.T01:exon:10288;Parent=MsG0780036528.01.T01 |
| Chr7 | exon | 9359122 | 9359284 | 9359122 | ID=MsG0780036528.01.T01:exon:10287;Parent=MsG0780036528.01.T01 |
| Chr7 | CDS | 9360136 | 9360278 | 9360136 | ID=MsG0780036528.01.T01:cds;Parent=MsG0780036528.01.T01 |
| Chr7 | CDS | 9359772 | 9360029 | 9359772 | ID=MsG0780036528.01.T01:cds;Parent=MsG0780036528.01.T01 |
| Chr7 | CDS | 9359416 | 9359673 | 9359416 | ID=MsG0780036528.01.T01:cds;Parent=MsG0780036528.01.T01 |
| Chr7 | CDS | 9359122 | 9359284 | 9359122 | ID=MsG0780036528.01.T01:cds;Parent=MsG0780036528.01.T01 |
| Gene Sequence |
| Protein sequence |
