Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036958.01.T01 | XP_024639353.1 | 58.779 | 131 | 52 | 1 | 8 | 136 | 1 | 131 | 3.01E-47 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036958.01.T01 | G7JX45 | 58.824 | 85 | 33 | 1 | 54 | 136 | 25 | 109 | 1.99e-25 | 102 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780036958.01.T01 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036958.01.T01 | MTR_5g053510 | 58.824 | 85 | 33 | 1 | 54 | 136 | 25 | 109 | 5.05e-29 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAATTCATCAGTGAATT+TGG | 0.118087 | 7:-17150273 | None:intergenic |
| ACAATGTGTCTTTAGGAATT+TGG | 0.142567 | 7:+17150240 | None:intergenic |
| CCGATTTCAATGATTTCTTA+TGG | 0.230421 | 7:+17150725 | MsG0780036958.01.T01:CDS |
| ATCAATTCATCAGTGAATTT+GGG | 0.249190 | 7:-17150272 | None:intergenic |
| CCAACCCTTCGTTAGCTTGC+TGG | 0.293858 | 7:-17150836 | None:intergenic |
| CCATAAGAAATCATTGAAAT+CGG | 0.346978 | 7:-17150725 | None:intergenic |
| GCACATCTCCAGCCTGATAT+TGG | 0.349705 | 7:-17150879 | None:intergenic |
| CACATTGACCCCTTTGCAAA+TGG | 0.355395 | 7:+17150566 | MsG0780036958.01.T01:CDS |
| CTGCACAACCAGCCAATATC+AGG | 0.358399 | 7:+17150867 | MsG0780036958.01.T01:CDS |
| CTGCGTTTCAAGTTCTTTGA+TGG | 0.370866 | 7:+17150901 | MsG0780036958.01.T01:CDS |
| GACTATTTGACCATTGTCAT+TGG | 0.378913 | 7:-17150776 | None:intergenic |
| CTGATTCTGTTTAGCTATGT+TGG | 0.402336 | 7:+17150431 | MsG0780036958.01.T01:exon |
| AGACGAACACACAACTTGAT+TGG | 0.422524 | 7:-17150976 | None:intergenic |
| TTCAATGATTTCTTATGGAA+TGG | 0.456862 | 7:+17150730 | MsG0780036958.01.T01:CDS |
| AATGGATGCGAATTCTGAAT+TGG | 0.461274 | 7:+17150584 | MsG0780036958.01.T01:CDS |
| TCGTGGATTACATCACTGAT+TGG | 0.474442 | 7:-17151062 | None:intergenic |
| GAAGTCTTGTGCTTCTGCTA+TGG | 0.476473 | 7:-17150515 | None:intergenic |
| ATCTCCAGCCTGATATTGGC+TGG | 0.482605 | 7:-17150875 | None:intergenic |
| AATTCGCATCCATTTGCAAA+GGG | 0.485601 | 7:-17150575 | None:intergenic |
| CTGCCACGGTTGACTAATGC+TGG | 0.487112 | 7:+17150369 | MsG0780036958.01.T01:five_prime_UTR |
| TTGATGACTTGGGTCCAGTC+TGG | 0.491750 | 7:+17150290 | MsG0780036958.01.T01:five_prime_UTR |
| CATAAGAAATCATTGAAATC+GGG | 0.494072 | 7:-17150724 | None:intergenic |
| TCAGCTTCATACCTTCAATC+TGG | 0.497612 | 7:-17150412 | None:intergenic |
| CAGCAAGCTAACGAAGGGTT+GGG | 0.497952 | 7:+17150837 | MsG0780036958.01.T01:CDS |
| AAAGTAGATTTGTTATCACT+TGG | 0.513327 | 7:+17150456 | MsG0780036958.01.T01:CDS |
| ATCACTTGGGAATACAATTG+AGG | 0.516429 | 7:+17150470 | MsG0780036958.01.T01:CDS |
| CCAGCAAGCTAACGAAGGGT+TGG | 0.517788 | 7:+17150836 | MsG0780036958.01.T01:CDS |
| TGCGTTTCAAGTTCTTTGAT+GGG | 0.523138 | 7:+17150902 | MsG0780036958.01.T01:CDS |
| CATAAAGACATTTGAAACAA+AGG | 0.528351 | 7:-17151027 | None:intergenic |
| ACTGCTATTCATTACCAAGT+TGG | 0.542667 | 7:+17150802 | MsG0780036958.01.T01:CDS |
| AAGTAGATTTGTTATCACTT+GGG | 0.552539 | 7:+17150457 | MsG0780036958.01.T01:CDS |
| TGACTAATGCTGGACAGACT+TGG | 0.558348 | 7:+17150379 | MsG0780036958.01.T01:five_prime_UTR |
| AATCATTGAAATCGGGTGGA+AGG | 0.560043 | 7:-17150717 | None:intergenic |
| TGGAATTTAATTGACGCTGA+AGG | 0.570875 | 7:+17150310 | MsG0780036958.01.T01:five_prime_UTR |
| GAATTCGCATCCATTTGCAA+AGG | 0.571229 | 7:-17150576 | None:intergenic |
| TCACTGATGAATTGATGACT+TGG | 0.573597 | 7:+17150279 | MsG0780036958.01.T01:five_prime_UTR |
| TCAATTCATCAGTGAATTTG+GGG | 0.578440 | 7:-17150271 | None:intergenic |
| GCGTCAATTAAATTCCAGAC+TGG | 0.579334 | 7:-17150304 | None:intergenic |
| TTGTTCCTCTCCAATGACAA+TGG | 0.585814 | 7:+17150766 | MsG0780036958.01.T01:CDS |
| GAACACACAACTTGATTGGA+AGG | 0.585955 | 7:-17150972 | None:intergenic |
| AAGAAATCATTGAAATCGGG+TGG | 0.589665 | 7:-17150721 | None:intergenic |
| CACTGATGAATTGATGACTT+GGG | 0.599913 | 7:+17150280 | MsG0780036958.01.T01:five_prime_UTR |
| TTTGACCATTGTCATTGGAG+AGG | 0.609768 | 7:-17150771 | None:intergenic |
| ACAATTCAGCTACTCTGCCA+CGG | 0.617034 | 7:+17150355 | MsG0780036958.01.T01:five_prime_UTR |
| ACTCACCAGCAAGCTAACGA+AGG | 0.624585 | 7:+17150831 | MsG0780036958.01.T01:CDS |
| CGCTGAAGGAAATTCTCATG+AGG | 0.625269 | 7:+17150324 | MsG0780036958.01.T01:five_prime_UTR |
| GTGCTTCTGCTATGGTACGT+AGG | 0.636405 | 7:-17150507 | None:intergenic |
| CAAGCTAACGAAGGGTTGGG+AGG | 0.643100 | 7:+17150840 | MsG0780036958.01.T01:CDS |
| ACAACCAGCCAATATCAGGC+TGG | 0.644275 | 7:+17150871 | MsG0780036958.01.T01:CDS |
| CTCACCAGCAAGCTAACGAA+GGG | 0.650798 | 7:+17150832 | MsG0780036958.01.T01:CDS |
| ATTCGCATCCATTTGCAAAG+GGG | 0.653436 | 7:-17150574 | None:intergenic |
| TGGTGAGTGAGTCACCAACT+TGG | 0.680717 | 7:-17150816 | None:intergenic |
| AACAAAATATAAGTTCATCG+TGG | 0.683011 | 7:-17151079 | None:intergenic |
| TGTCCAGCATTAGTCAACCG+TGG | 0.725012 | 7:-17150372 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACACAATGAAAATTAAGATT+AGG | - | Chr7:17150685-17150704 | None:intergenic | 20.0% |
| !! | TGTTTATTAAAATCTGCAAA+TGG | + | Chr7:17150635-17150654 | MsG0780036958.01.T01:intron | 20.0% |
| ! | AACAAAATATAAGTTCATCG+TGG | - | Chr7:17151082-17151101 | None:intergenic | 25.0% |
| ! | ATCAATTCATCAGTGAATTT+GGG | - | Chr7:17150275-17150294 | None:intergenic | 25.0% |
| ! | CATAAGAAATCATTGAAATC+GGG | - | Chr7:17150727-17150746 | None:intergenic | 25.0% |
| ! | CCATAAGAAATCATTGAAAT+CGG | - | Chr7:17150728-17150747 | None:intergenic | 25.0% |
| ! | TTCAATGATTTCTTATGGAA+TGG | + | Chr7:17150730-17150749 | MsG0780036958.01.T01:CDS | 25.0% |
| !! | CATAAAGACATTTGAAACAA+AGG | - | Chr7:17151030-17151049 | None:intergenic | 25.0% |
| !!! | AAAGTAGATTTGTTATCACT+TGG | + | Chr7:17150456-17150475 | MsG0780036958.01.T01:CDS | 25.0% |
| !!! | AAGTAGATTTGTTATCACTT+GGG | + | Chr7:17150457-17150476 | MsG0780036958.01.T01:CDS | 25.0% |
| !!! | TGCAGATTTTAATAAACACA+TGG | - | Chr7:17150633-17150652 | None:intergenic | 25.0% |
| AGATTAGGTGTAAAAAGAGT+TGG | - | Chr7:17150670-17150689 | None:intergenic | 30.0% | |
| CATCAATTCATCAGTGAATT+TGG | - | Chr7:17150276-17150295 | None:intergenic | 30.0% | |
| CCGATTTCAATGATTTCTTA+TGG | + | Chr7:17150725-17150744 | MsG0780036958.01.T01:CDS | 30.0% | |
| TCAATTCATCAGTGAATTTG+GGG | - | Chr7:17150274-17150293 | None:intergenic | 30.0% | |
| ! | TTTTCATTGTGTCTAAATGC+AGG | + | Chr7:17150693-17150712 | MsG0780036958.01.T01:intron | 30.0% |
| !!! | CAACATGTTCCCTTTTTTTT+AGG | + | Chr7:17151107-17151126 | MsG0780036958.01.T01:three_prime_UTR | 30.0% |
| AAGAAATCATTGAAATCGGG+TGG | - | Chr7:17150724-17150743 | None:intergenic | 35.0% | |
| AATGGATGCGAATTCTGAAT+TGG | + | Chr7:17150584-17150603 | MsG0780036958.01.T01:CDS | 35.0% | |
| AATTCGCATCCATTTGCAAA+GGG | - | Chr7:17150578-17150597 | None:intergenic | 35.0% | |
| ACTGCTATTCATTACCAAGT+TGG | + | Chr7:17150802-17150821 | MsG0780036958.01.T01:CDS | 35.0% | |
| ATCACTTGGGAATACAATTG+AGG | + | Chr7:17150470-17150489 | MsG0780036958.01.T01:CDS | 35.0% | |
| CTGATTCTGTTTAGCTATGT+TGG | + | Chr7:17150431-17150450 | MsG0780036958.01.T01:exon | 35.0% | |
| GACTATTTGACCATTGTCAT+TGG | - | Chr7:17150779-17150798 | None:intergenic | 35.0% | |
| TGGAATTTAATTGACGCTGA+AGG | + | Chr7:17150310-17150329 | MsG0780036958.01.T01:five_prime_UTR | 35.0% | |
| ! | CACTGATGAATTGATGACTT+GGG | + | Chr7:17150280-17150299 | MsG0780036958.01.T01:five_prime_UTR | 35.0% |
| ! | GCACAATTTTACCAGATTGA+AGG | + | Chr7:17150401-17150420 | MsG0780036958.01.T01:five_prime_UTR | 35.0% |
| ! | TCACTGATGAATTGATGACT+TGG | + | Chr7:17150279-17150298 | MsG0780036958.01.T01:five_prime_UTR | 35.0% |
| !! | TGCGTTTCAAGTTCTTTGAT+GGG | + | Chr7:17150902-17150921 | MsG0780036958.01.T01:CDS | 35.0% |
| AATCATTGAAATCGGGTGGA+AGG | - | Chr7:17150720-17150739 | None:intergenic | 40.0% | |
| AGACGAACACACAACTTGAT+TGG | - | Chr7:17150979-17150998 | None:intergenic | 40.0% | |
| ATTCGCATCCATTTGCAAAG+GGG | - | Chr7:17150577-17150596 | None:intergenic | 40.0% | |
| GAACACACAACTTGATTGGA+AGG | - | Chr7:17150975-17150994 | None:intergenic | 40.0% | |
| GAATTCGCATCCATTTGCAA+AGG | - | Chr7:17150579-17150598 | None:intergenic | 40.0% | |
| GCGTCAATTAAATTCCAGAC+TGG | - | Chr7:17150307-17150326 | None:intergenic | 40.0% | |
| TCAGCTTCATACCTTCAATC+TGG | - | Chr7:17150415-17150434 | None:intergenic | 40.0% | |
| TTGTTCCTCTCCAATGACAA+TGG | + | Chr7:17150766-17150785 | MsG0780036958.01.T01:CDS | 40.0% | |
| TTTGACCATTGTCATTGGAG+AGG | - | Chr7:17150774-17150793 | None:intergenic | 40.0% | |
| ! | TCGTGGATTACATCACTGAT+TGG | - | Chr7:17151065-17151084 | None:intergenic | 40.0% |
| !! | CTGCGTTTCAAGTTCTTTGA+TGG | + | Chr7:17150901-17150920 | MsG0780036958.01.T01:CDS | 40.0% |
| ACAATTCAGCTACTCTGCCA+CGG | + | Chr7:17150355-17150374 | MsG0780036958.01.T01:five_prime_UTR | 45.0% | |
| CACATTGACCCCTTTGCAAA+TGG | + | Chr7:17150566-17150585 | MsG0780036958.01.T01:CDS | 45.0% | |
| CGCTGAAGGAAATTCTCATG+AGG | + | Chr7:17150324-17150343 | MsG0780036958.01.T01:five_prime_UTR | 45.0% | |
| TGACTAATGCTGGACAGACT+TGG | + | Chr7:17150379-17150398 | MsG0780036958.01.T01:five_prime_UTR | 45.0% | |
| ! | GAAGTCTTGTGCTTCTGCTA+TGG | - | Chr7:17150518-17150537 | None:intergenic | 45.0% |
| ACAACCAGCCAATATCAGGC+TGG | + | Chr7:17150871-17150890 | MsG0780036958.01.T01:CDS | 50.0% | |
| ACTCACCAGCAAGCTAACGA+AGG | + | Chr7:17150831-17150850 | MsG0780036958.01.T01:CDS | 50.0% | |
| ATCTCCAGCCTGATATTGGC+TGG | - | Chr7:17150878-17150897 | None:intergenic | 50.0% | |
| CAGCAAGCTAACGAAGGGTT+GGG | + | Chr7:17150837-17150856 | MsG0780036958.01.T01:CDS | 50.0% | |
| CTCACCAGCAAGCTAACGAA+GGG | + | Chr7:17150832-17150851 | MsG0780036958.01.T01:CDS | 50.0% | |
| CTGCACAACCAGCCAATATC+AGG | + | Chr7:17150867-17150886 | MsG0780036958.01.T01:CDS | 50.0% | |
| GCACATCTCCAGCCTGATAT+TGG | - | Chr7:17150882-17150901 | None:intergenic | 50.0% | |
| GTGCTTCTGCTATGGTACGT+AGG | - | Chr7:17150510-17150529 | None:intergenic | 50.0% | |
| TGGTGAGTGAGTCACCAACT+TGG | - | Chr7:17150819-17150838 | None:intergenic | 50.0% | |
| TGTCCAGCATTAGTCAACCG+TGG | - | Chr7:17150375-17150394 | None:intergenic | 50.0% | |
| ! | TTGATGACTTGGGTCCAGTC+TGG | + | Chr7:17150290-17150309 | MsG0780036958.01.T01:five_prime_UTR | 50.0% |
| CAAGCTAACGAAGGGTTGGG+AGG | + | Chr7:17150840-17150859 | MsG0780036958.01.T01:CDS | 55.0% | |
| CCAGCAAGCTAACGAAGGGT+TGG | + | Chr7:17150836-17150855 | MsG0780036958.01.T01:CDS | 55.0% | |
| CTGCCACGGTTGACTAATGC+TGG | + | Chr7:17150369-17150388 | MsG0780036958.01.T01:five_prime_UTR | 55.0% | |
| !! | CCAACCCTTCGTTAGCTTGC+TGG | - | Chr7:17150839-17150858 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17150253 | 17151126 | 17150253 | ID=MsG0780036958.01;Name=MsG0780036958.01 |
| Chr7 | mRNA | 17150253 | 17151126 | 17150253 | ID=MsG0780036958.01.T01;Parent=MsG0780036958.01;Name=MsG0780036958.01.T01;_AED=0.00;_eAED=0.00;_QI=194|1|1|1|1|1|2|160|136 |
| Chr7 | exon | 17150253 | 17150605 | 17150253 | ID=MsG0780036958.01.T01:exon:3176;Parent=MsG0780036958.01.T01 |
| Chr7 | exon | 17150715 | 17151126 | 17150715 | ID=MsG0780036958.01.T01:exon:3177;Parent=MsG0780036958.01.T01 |
| Chr7 | five_prime_UTR | 17150253 | 17150446 | 17150253 | ID=MsG0780036958.01.T01:five_prime_utr;Parent=MsG0780036958.01.T01 |
| Chr7 | CDS | 17150447 | 17150605 | 17150447 | ID=MsG0780036958.01.T01:cds;Parent=MsG0780036958.01.T01 |
| Chr7 | CDS | 17150715 | 17150966 | 17150715 | ID=MsG0780036958.01.T01:cds;Parent=MsG0780036958.01.T01 |
| Chr7 | three_prime_UTR | 17150967 | 17151126 | 17150967 | ID=MsG0780036958.01.T01:three_prime_utr;Parent=MsG0780036958.01.T01 |
| Gene Sequence |
| Protein sequence |
